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... It is difficult to quantify the mechanism(s) responsible for competition-induced yield loss using traditional experimental techniques. A technique using yield and ¹³C discrimination (ΔΔ) for wheat, a C₃ plant, has been developed to separate total yield loss (TYL) into yield loss due to N (YLNS) and water (YLWS) stresses. The objective of this research was to determine whether the ΔΔ approach could ...
... Natural variations of the 13C/12C ratio have been frequently used over the last three decades to trace C sources and fluxes between plants, microorganisms, and soil. Many of these studies have used the natural-13C-labelling approach, i.e. natural δ13C variation after C3–C4 vegetation changes. In this review, we focus on 13C fractionation in main processes at the interface between roots, microorgan ...
C3 plants; C4 plants; Cyperus esculentus; Digitaria sanguinalis; Echinochloa crus-galli; Leptochloa fusca subsp. fascicularis; Panicum; carbon; equations; grasses; greenhouses; isotopes; leaves; paddies; red rice; roots; shoots; soil; weeds; United States
Abstract:
... Assessing belowground plant interference in rice has been difficult in the past because intertwined weed and crop roots cannot be readily separated. A 13C discrimination method has been developed to assess distribution of intermixed roots of barnyardgrass and rice in field soils, but the suitability of this approach for other rice weeds is not known. 13C depletion levels in roots and leaves of ric ...
... On the basis of multi-proxy analysis on TOC, TN, C/N, organic δ¹³C and grain size, sediment record from Qinghai Lake provides evidences of stepwise-patterned climatic change since 16 ka BP. Results show that Qinghai Lake underwent six environmental stages. From 16.2 to 14.3 ka BP and from 4.0 to 2.1 ka BP, the organic δ¹³C value was controlled by the concentration of atmospheric CO₂. Relative high ...
Marco M. Lehmann; Gregory R. Goldsmith; Cathleen Mirande‐Ney; Rosemarie B. Weigt; Leonie Schönbeck; Ansgar Kahmen; Arthur Gessler; Rolf T.W. Siegwolf; Matthias Saurer
... The ¹⁸O signature of atmospheric water vapour (δ¹⁸OV) is known to be transferred via leaf water to assimilates. It remains, however, unclear how the ¹⁸O‐signal transfer differs among plant species and growth forms. We performed a 9‐hr greenhouse fog experiment (relative humidity ≥ 98%) with ¹⁸O‐depleted water vapour (−106.7‰) on 140 plant species of eight different growth forms during daytime. We ...
C4 plants; color; grasses; organic matter; paleoclimatology; soil; stable isotopes; summer; temperature; Greenland; South Africa
Abstract:
... High-resolution stable isotope variations and growth structure analyses of the last three millennia of a 6600-year stalagmite record at Cold Air Cave, Makapansgat Valley, South Africa, are presented. Growth layers, which are measurable over the last 250 years, are shown to be annual. The correlation between the width of growth layers and precipitation is strongly positive. Changes inδ¹⁸O andδ¹³C a ...
... The study of functional trait plasticity and optimal allocation strategies in a water competition context may help to explain the mechanisms relating plant competition to ecological semi-arid patterns. We measured four functional plant traits –root to shoot ratio, superficial to deep roots ratio, root diameter, and root estimated surface area to shoot ratio– in wild populations of two C4 grasses i ...
... We evaluated the influences of CO2[Control, approximately 370 micromol mol-1; 200 micromol mol-1 above ambient applied by free-air CO2 enrichment (FACE)] and soil water (Wet, Dry) on above- and below-ground responses of C3 (cotton, Gossypium hirsutum) and C4 (sorghum, Sorghum bicolor) plants in monocultures and two density mixtures. In monocultures, CO2 enrichment increased height, leaf area, abov ...
... Plant species in grasslands are often separated into groups (C₄ and C₃ grasses, and forbs) with presumed links to ecosystem functioning. Each of these in turn can be separated into native and introduced (i.e., exotic) species. Although numerous studies have compared plant traits between the traditional groups of grasses and forbs, fewer have compared native versus introduced species. Introduced gr ...
... AIM: To reconstruct ecological changes from the fossil record of a unique wetland on the tropical oceanic island of Tahiti, between 44.5 and 38 cal. kyr bp. LOCATION: Vaifanaura'amo'ora, Tamanu Plateau, Punaru'u Valley, Tahiti, Society Islands, French Polynesia (17°38′S, 149°32′50″E). METHODS: Fossil pollen, spores, seeds, diatoms and invertebrates were examined from a 3.7 m core consisting of Ple ...
... Ecosystems with alternative attractors are susceptible to abrupt regime shifts that are often difficult to predict and reverse. In this study, we quantify multiple system dynamics to determine whether the transition of mesic grassland to shrubland, a widespread phenomenon, represents a linear reversible process, a nonlinear but reversible threshold process, or a transition between alternative attr ...
... Invasive plant species often dominate native species in competition, augmenting other potential advantages such as release from natural enemies. Resource pre-emption may be a particularly important mechanism for establishing dominance over competitors of the same functional type. We hypothesized that competitive success of an exotic grass against native grasses is mediated by establishing an early ...
... C4 plants have a biochemical carbon-concentrating mechanism that increases CO2 concentration around Rubisco in the bundle sheath. Under low light, the activity of the carbon-concentrating mechanism generally decreases, associated with an increase in leakiness (ϕ), the ratio of CO2 retrodiffusing from the bundle sheath relative to C4 carboxylation. This increase in ϕ had been theoretically associat ...
... C4 plants have a biochemical carbon concentrating mechanism (CCM) that increases CO₂ concentration around ribulose bisphosphate carboxylase oxygenase (Rubisco) in the bundle sheath (BS). Under limiting light, the activity of the CCM generally decreases, causing an increase in leakiness, (Φ), the ratio of CO₂ retrodiffusing from the BS relative to C4 carboxylation processes. Maize plants were grown ...
... The accident at Fukushima 1 Nuclear Power Plant in 2011 caused contamination by radioactive cesium (¹³⁴Cs and ¹³⁷Cs) in surrounding areas. After this accident, concerns about Cs contamination, including food safety, have limited industrial activities and reconstruction in Fukushima. Sorghum (Sorghum bicolor (L.) Moench) is an annual C₄ crop that can be used as biofuel feedstock due to its high bio ...
... By concentrating CO₂, C₄ photosynthesis can suppress photorespiration and achieve high photosynthetic efficiency, especially under conditions of high light, high temperature, and drought. To concentrate CO₂, extra ATP is required, which would also require a change in photosynthetic electron transport in C₄ photosynthesis from that in C₃ photosynthesis. Several analyses have shown that the accumula ...
C4 plants; Miscanthus giganteus; Panicum virgatum; biofuels; biomass production; crop management; ecotypes; energy crops; environmental factors; feedstocks; fertilizer rates; growth performance; highlands; lowlands; models; nitrogen fertilizers; perennials; provenance; soil; soil fertility; Great Plains region; United States
Abstract:
... As high productivities of perennial grasses are evaluated for feedstock, a major consideration is biomass stability. In this study, two experiments were conducted to examine some components of this for two biofuel species: switchgrass (Panicum vigratum L.) and Miscanthus x giganteus (Mxg). The potential yields of these two species were evaluated under various environmental conditions across Southe ...
... The effect of salinity on C₄ photosynthesis was examined in leaves of maize, a NADP-malic enzyme (NADP-ME) type C₄ species. Potted plants with the fourth leaf blade fully developed were treated with 3% NaCl solution for 5d. Under salt treatment, the activities of pyruvate orthophosphate dikinase (PPDK), phosphoenolpyruvate carboxylase (PEPCase), NADP-dependent malate dehydrogenase (NADP-MDH) and N ...
C3 plants; C4 plants; Helianthus annuus; Zea mays; agricultural productivity; corn; drought; drought tolerance; food security; gas exchange; heat; heat stress; leaf area; photosynthesis; plant available water; plant growth; ribulose-bisphosphate carboxylase; root systems; semiarid zones; stomatal conductance; temperature; water stress; water use efficiency
Abstract:
... Heat and drought stress frequently occur together, however, their impact on plant growth and photosynthesis (PN) is unclear. The frequency, duration and severity of heat and drought stress events are predicted to increase in the future, having severe implications for agricultural productivity and food security. To assess the impact on plant gas exchange, physiology and morphology we grew drought t ...
... • Whole‐plant morphology, leaf structure and composition were studied together with the effects of light orientation on the dorso‐ventral regulation of photosynthesis and stomatal conductance in Paspalum dilatatum cv. Raki plants grown for 6 wk at either 350 or 700 µl l⁻¹ CO₂. • Plant biomass was doubled as a result of growth at high CO₂ and the shoot:root ratio was decreased. Stomatal density was ...
... The mid-Late Pleistocene and Holocene aeolianites of the Tamala Limestone on the northern Swan Coastal Plain in southwestern Western Australia consist of six members that show cyclic deposition of coastal aeolianite, overlain by calcrete/microbialite, karstified surface and palaeosol. Field work, combined with mineralogical, chemical, stable isotope analysis and uranium-thorium (U/Th) and opticall ...
C4 plants; paleoecology; plant cuticle; carbon; water temperature; waxes; alkanes; community ecology; plant ecology; stable isotopes; vegetation; paleoclimatology; Central Africa
Abstract:
... The dominant forcing factors for past large-scale changes in vegetation are widely debated. Changes in the distribution of C4 plants--adapted to warm, dry conditions and low atmospheric CO2 concentrations--have been attributed to marked changes in environmental conditions, but the relative impacts of changes in aridity, temperature and CO2 concentration are not well understood. Here, we present a ...
... Decomposition of soil organic matter (SOM) protected within aggregates can be accelerated via priming effect (PE) by the addition of fresh substrates. However, the knowledge of the sources of mineralization and PE in aggregate size classes is absent. We applied the three-source-partitioning isotopic (14C + δ13C) approach to determine how aggregate size classes affect the contribution of three C so ...
... C₄ plants have two types of photosynthetic cells, mesophyll (M) and bundle sheath (BS) cells with different subcellular structures. In C₄ plants, maize and finger millet, the chloroplasts of M cells are normally distributed toward the periphery but redistribute toward the BS cell side in response to environmental stresses. This directional movement of M chloroplasts is called aggregative movement, ...
... Two primary approaches to perennial biofuel crop production studied so far are fertilized grass monocultures and low-input high-diversity grasslands. While high-yielding perennial grass varieties are being developed in fertilized monocultures, breeding for yield in low-input high-diversity systems would be difficult. Before initiating breeding for low-input systems, it is therefore important to kn ...
Dongsheng Zhang; Guijuan Du; Zhanxiang Sun; Wei Bai; Qi Wang; Liangshan Feng; Jiaming Zheng; Zhe Zhang; Yang Liu; Shu Yang; Ning Yang; Chen Feng; Qian Cai; Jochem B. Evers; Wopke van der Werf; Lizhen Zhang
... Agroforestry systems, which combine annual crops with trees, are used widely in semi-arid regions to reduce wind erosion and improve resource (e.g. water) use efficiency. Limited knowledge is available on optimizing such systems by the choice of crop species with specific physiological traits (i.e. C3 vs C4, N-fixing vs non-N-fixing). In this study we quantified the light interception and utilizat ...
C3 plants; C4 plants; alcohol drinking; beers; carbon; disinfectants; ethanol; mass spectrometry; stable isotopes; China
Abstract:
... The origin of ethanol detected in bio-samples whether it be from the consumption of alcoholic beverages or contamination with disinfectants has been questioned in court cases in China recently. The stable carbon isotope naturally occurs in carbon-containing compounds and can help determine the origin of the compound in question. In total, 42 types of beers and 11 types of disinfectants were analyz ...
... This study developed a set of algorithms for satellite mapping of green leaf area index (LAI) in C3 and C4 crops. In situ hyperspectral reflectance and green LAI data, collected across eight years (2001–2008) at three AmeriFlux sites in Nebraska USA over irrigated and rain-fed maize and soybean, were used for algorithm development. The hyperspectral reflectance was resampled to simulate the spectr ...
C4 plants; Dinophyceae; alkanes; biomarkers; carbon; climate; climate change; forests; grasses; isotopes; lakes; leaves; lipids; macrophytes; monsoon season; rain; sediments; summer; trees; water stress; water use efficiency; watersheds; China
Abstract:
... Annually laminated sediments in Lake Xiaolongwan, northeast China, contain a suite of n-alkanes (C₁₇–C₃₃) with a strong odd over even carbon number predominance. Biomarker n-alkane proxies (average chain length, Paq ratio, grass/tree ratio, carbon preference index and compound-specific δ¹³C values) were used to reconstruct climate changes that occurred over the last millennium. Compound-specific δ ...
... Background: African C4 grasses threatens newly restored areas in Neotropical savannas. Allelopathy exhibited by some native species may contribute to ecological restoration. Aims: To establish the potential use of Lepidaploa aurea (Asteraceae) in ecological restoration of invaded savannas, we evaluated its allelopathic effects on two invasive grasses, a native grass, and two native trees (one sava ...
... Introducing enzymes involved in photosynthesis of C₄ plants into rice (Oryza sativa L.) is supposed to enhance the photosynthesis and crop productivity. However, only a few researches showed that the photosynthesis and crop productivity have been improved by introducing phosphoenolpyruvate carboxylase (PEPC) gene into rice. In the present study, the photosynthetic rates (Pₙ) in 42 rice lines overe ...
Youping Zhou; Hilary Stuart-Williams; Kliti Grice; Zachary E. Kayler; Saša Zavadlav; Angela Vogts; Florian Rommerskirchen; Graham D. Farquhar; Arthur Gessler
... It has long been theorized that carbon allocation, in addition to the carbon source and to kinetic isotopic effects associated with a particular lipid biosynthetic pathway, plays an important role in shaping the carbon isotopic composition (13C/12C) of lipids (Park and Epstein, 1961). If the latter two factors are properly constrained, valuable information about carbon allocation during lipid bios ...
... Because allochthonous organic matter (OM) loading supplements autochthonous OM in supporting lake and reservoir food webs, C and N elemental and isotopic ratios of sedimenting particulate OM were measured during an annual cycle in a polymictic, eutrophic reservoir. Particulate organic C and N deposition rates were greatest during winter and lowest during spring. C:N ratios decreased through our st ...
C4 plants; Cenchrus purpureus; aboveground biomass; allometry; altitude; bioenergy; carbon; carbon sinks; data collection; decision making; energy cane; environmental sustainability; equations; feedstocks; grasses; greenhouse gases; land management; leaves; mineral resources; model validation; models; perennials; prediction; rain; sugarcane; tropics; Maui
Abstract:
... Biomass is a promising renewable energy option that provides a more environmentally sustainable alternative to fossil resources by reducing the net flux of greenhouse gasses to the atmosphere. Yet, allometric models that allow the prediction of aboveground biomass (AGB), biomass carbon (C) stock non-destructively have not yet been developed for tropical perennial C4 grasses currently under conside ...
... Several thousand plant genes are known to produce multiple transcripts, but the precise function of most of the alternatively encoded proteins is not known. Alternative splicing has been reported for the H-protein subunit of glycine decarboxylase in the genus Flaveria. H-protein has no catalytic activity itself but is a substrate of the three enzymatically active subunits, P-, T- and L-protein. In ...
... Centipedegrass (Eremochloa ophiuroides [Munro] Hack.) is a C4 perennial grass and well adapted to acid soils. Centipedegrass accession E041 (Lingchuan, Guangxi Province, China) was found to be more tolerant to Aluminum (Al) than E006 (Nanjing, Jiangsu Province, China). The objective of this study was to identify possible mechanisms associated with centipedegrass. After exposure to Al, citrate was ...
... Peatlands store large amounts of soil organic carbon (SOC). Drainage, required for agriculture, aerates these organic soils and triggers rapid peat decomposition. In turn, cultivation of organic soils is also accompanied by input of young organic carbon (YOC) from plant residues. The extent to which YOC inputs compensate for oxidative peat loss is unknown. Furthermore, the lability of YOC in organ ...
... Tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), is the major insect pest of cotton, Gossypium hirsutum L., in the mid-South. This pest moves into cotton fields from non-cotton hosts during late spring and early summer. Stable carbon isotope (SCI) analysis was used to characterize plant hosts of tarnished plant bugs, as well as adult tarnished plant bugs that had developed as immatures ...
Agropyron; Bouteloua curtipendula; C4 plants; experimental design; grasses; grasslands; night temperature; nitrogen; nutrient use efficiency; soil temperature
Abstract:
... The replacement series experimental design and mathematical procedures of de Wit were used to study the influence of soil nitrogen supply and temperature on the growth, in monoculture and in mixture, of two North American grassland species, Agropyran smithii (a C₃ pathway species) and Bouteloua curtipendula (C₄). At 20/12°C day/night temperatures, direct competition for soil nitrogen resulted in l ...
... Bundle sheath chloroplasts of NADP-malic enzyme (NADP-ME) type C₄ species have a high demand for ATP, while being deficient in linear electron flow and oxidation of water by photosystem II (PSII). To evaluate electron donors to photosystem I (PSI) and possible pathways of cyclic electron flow (CEF1) in isolated bundle sheath strands of maize (Zea mays L.), an NADP-ME species, light-induced redox k ...
... More efficient photosynthesis has allowed C₄ plants to adapt to more diverse ecosystems (such as hot and arid conditions) than C₃ plants. To better understand C₄ photosynthesis, we investigated the expression patterns of C₄ genes (C4PPDK and PCK1) and their non-C₄ homologous genes (CyPPDK1, CyPPDK2, and PCK2) in the different organs of maize (Zea mays). Both C₄ genes and non-C₄ genes showed organ- ...
... Chloroplast-localized small heat-shock proteins (Cp-sHSP) protect Photosystem II and thylakoid membranes during heat and other stresses, and Cp-sHSP production levels are related to plant thermotolerance. However, to date, a paucity of Cp-sHSP sequences from C4 or CAM species, or from other extremely heat-tolerant species, has precluded an examination to determine if Cp-sHSP genes or proteins migh ...
... In order to investigate the effects, without competition, of CO₂ on germination, growth, physiological response, and reproduction, we focussed on co—occurring species that are prominent members of an annual community in Illinois. Five species of old field annual plants—Abutilon theophrasti (C₃), Amaranthus retroflexus (C₄), Ambrosia artemisiifolia (C₃), Chenopodium album (C₃), and Setaria faberii ...
... In C₄ photosynthesis CO₂ assimilation and reduction are typically coordinated across mesophyll (M) and bundle sheath (BS) cells, respectively. This system consequently requires sufficient light to reach BS to generate enough ATP to allow ribulose‐1,5‐bisphosphate (RuBP) regeneration in BS. Leaf anatomy influences BS light penetration and therefore constrains C₄ cycle functionality. Using an absorp ...
... Setaria viridis is being promoted as a model C₄ photosynthetic plant because it has a small genome (~515 Mb), a short life cycle (~60 d) and it can be transformed. Unlike other C₄ grasses such as maize, however, there is very little information about how C₄ leaf anatomy (Kranz anatomy) develops in S. viridis. As a foundation for future developmental genetic studies, we provide an anatomical and ul ...
... Gross primary productivity (GPP) is a vital ecosystem variable that is used as a proxy to study the functional behaviour of a terrestrial ecosystem and its ability to regulate atmospheric CO₂ by working as a carbon pool. India, having the potential terrestrial ecosystem dynamics to absorb the atmospheric carbon dioxide to some extent, is one of the least-explored regions in terms of carbon monitor ...
... Notiomastodon platensis was an endemic South American proboscidean, which could reach about six tons and had a mixed feeder diet with predominance of C₄ grasses in Brazilian Intertropical Region (BIR). In the last decades, its feeding behavior has been rebuilt mainly through dental calculus, enamel microwear and stable isotope analyses. However, all these techniques allow us to know only punctual ...
... QUESTIONS: Disturbance regimes shaped the evolution of grasslands but grazing exclusion and fire suppression have caused unprecedented increases in grassland biomass and biodiversity declines. Mowing reduces biomass but is not widely practiced in conservation reserves because of concerns about facilitating exotic plant invasion and altering ecosystem function. We asked whether regular mowing affec ...
... On sites where C4-plants have replaced C3-plants, changes in soil δ¹³C allow the turnover of C3- and C4-derived C to be separated. Studies of decadal scale turnover of soil C following conversion to C4-plants generally lack δ¹³C values for previous C4-residue inputs and assume that estimates of C4-derived soil C to be based on a fixed δ¹³C value. Further assumptions are that changes in the initial ...