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1. A promiscuous T cell epitope-based HIV vaccine providing redundant population coverage of the HLA class II elicits broad, polyfunctional T cell responses in nonhuman primates

Author:
Susan Pereira Ribeiro; Vania Gomes De Moura Mattaraia; Rafael Ribeiro Almeida; Elizabeth Juliana Ghiuro Valentine; Natiely Silva Sales; Luís Carlos S. Ferreira; Luiz Carlos Sa-Rocha; Lucas Cauê Jacintho; Vinicius Canato Santana; John Sidney; Alessandro Sette; Daniela Santoro Rosa; Jorge Kalil; Edecio Cunha-Neto
Source:
Vaccine 2022 v.40 no.2 pp. 239-246
ISSN:
0264-410X
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; COVID-19 infection; HIV infections; cell-mediated immunity; electroporation; epitopes; genetically modified organisms; human population; humans; immunization; peptides; recombinant vaccines
Abstract:
... Over the last few decades, several emerging or reemerging viral diseases with no readily available vaccines have ravaged the world. A platform to fastly generate vaccines inducing potent and durable neutralizing antibody and T cell responses is sorely needed. Bioinformatically identified epitope-based vaccines can focus on immunodominant T cell epitopes and induce more potent immune responses than ...
DOI:
10.1016/j.vaccine.2021.11.076

2. Bioactive fish collagen peptides weaken intestinal inflammation by orienting colonic macrophages phenotype through mannose receptor activation

Author:
Mouna Rahabi; Marie Salon; Christelle Bruno-Bonnet; Mélissa Prat; Godefroy Jacquemin; Khaddouj Benmoussa; Mohamad Alaeddine; Mélissa Parny; José Bernad; Bénédicte Bertrand; Yannick Auffret; Pascale Robert-Jolimaître; Laurent Alric; Hélène Authier; Agnès Coste
Source:
European journal of nutrition 2022 v.61 no.4 pp. 2051-2066
ISSN:
1436-6207
Subject:
CD8-positive T-lymphocytes; antioxidants; chemiluminescence; colitis; collagen; cytokines; dextran; fish; flow cytometry; functional foods; humans; immunomodulation; inflammation; intestinal microorganisms; intestines; macrophages; mannose; monocytes; myeloperoxidase; phenotype; sodium sulfate
Abstract:
... PURPOSE: Particular interest is now given to the potential of dietary supplements as alternative non-pharmacological approaches in intestinal inflammation handling. In this aim, this study evaluates the efficiency of fish collagen peptides, Naticol®Gut, on colonic inflammation. METHODS: Wild type and Mannose receptor-deficient in the myeloid lineage C57BL/6 mice were administered with Dextran Sodi ...
DOI:
10.1007/s00394-021-02787-7

3. Characterization of humoral and cell-mediated immunity induced by mRNA vaccines expressing influenza hemagglutinin stem and nucleoprotein in mice and nonhuman primates

Author:
Jessica A. Flynn; Teresa Weber; Pedro J. Cejas; Kara S. Cox; Sinoeun Touch; Lauren A. Austin; Yangsi Ou; Michael P. Citron; Bin Luo; Marian E. Gindy; Kapil Bahl; Giuseppe Ciaramella; Amy S. Espeseth; Lan Zhang
Source:
Vaccine 2022 pp. -
ISSN:
0264-410X
Subject:
CD8-positive T-lymphocytes; Orthomyxoviridae; blood serum; cell-mediated immunity; cytotoxicity; hemagglutinins; immune response; immunogenicity; influenza; mice; morbidity; nucleoproteins; seroprevalence; vaccination; vaccines
Abstract:
... In response to immune pressure, influenza viruses evolve, producing drifted variants capable of escaping immune recognition. One strategy for inducing a broad-spectrum immune response capable of recognizing multiple antigenically diverse strains is to target conserved proteins or protein domains. To that end, we assessed the efficacy and immunogenicity of mRNA vaccines encoding either the conserve ...
DOI:
10.1016/j.vaccine.2022.03.063

4. Characterization of the cell-mediated immune response to Takeda’s live-attenuated tetravalent dengue vaccine in adolescents participating in a phase 2 randomized controlled trial conducted in a dengue-endemic setting

Author:
Vianney Tricou; Raphael Gottardo; Michael A. Egan; Frédéric Clement; Geert Leroux-Roels; Xavier Sáez-Llorens; Astrid Borkowski; Derek Wallace; Hansi J. Dean
Source:
Vaccine 2022 v.40 no.8 pp. 1143-1151
ISSN:
0264-410X
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; cell-mediated immunity; cross reaction; dengue; flow cytometry; interleukin-2; live vaccines; serotypes; Panama
Abstract:
... As robust dengue-specific CD4+ and CD8+ T cell responses are essential for protective immunity, we assessed cell-mediated immune (CMI) responses to a DENV-2-based dengue tetravalent vaccine candidate (TAK-003) in adolescents living in Panama, a dengue-endemic country. Peripheral blood mononuclear cells were collected from a subset of 67 participants ≥ 10 years old included in a phase 2 clinical tr ...
DOI:
10.1016/j.vaccine.2022.01.016

5. Current Understanding of the Role of T Cells in Chikungunya, Dengue and Zika Infections

Author:
Maheshi Mapalagamage; Daniela Weiskopf; Alessandro Sette; Aruna Dharshan De Silva
Source:
Viruses 2022 v.14 no.2 pp. -
ISSN:
1999-4915
Subject:
B-lymphocytes; CD8-positive T-lymphocytes; burden of disease; cross reaction; cytokines; dengue; epitopes; humans; lymph; protective effect; vaccines
Abstract:
... Arboviral infections such as Chikungunya (CHIKV), Dengue (DENV) and Zika (ZIKV) are a major disease burden in tropical and sub-tropical countries, and there are no effective vaccinations or therapeutic drugs available at this time. Understanding the role of the T cell response is very important when designing effective vaccines. Currently, comprehensive identification of T cell epitopes during a D ...
DOI:
10.3390/v14020242

6. Healthy myeloid-derived suppressor cells express the surface ectoenzyme Vanin-2 (VNN2)

Author:
David C. Soler; Amber Kerstetter-Fogle; Andrew B. Young; Pat Rayman; James H. Finke; Sarah M. Debanne; Kevin D. Cooper; Jill Barnholtz-Sloan; Andrew E. Sloan; Thomas S. McCormick
Source:
Molecular immunology 2022 v.142 pp. 1-10
ISSN:
0161-5890
Subject:
CD8-positive T-lymphocytes; biomarkers; cell proliferation; glioma; humans; immunology; microarray technology; monocytes; phenotype
Abstract:
... Study of human monocytic Myeloid-Derived Suppressor cells Mo-MDSC (CD14⁺ HLA-DRⁿᵉᵍ/ˡᵒʷ) has been hampered by the lack of positive cell-surface markers. In order to identify positive markers for Mo-MDSC, we performed microarray analysis comparing Mo-MDSC cells from healthy subjects versus CD14⁺ HLA-DRʰⁱᵍʰ monocytes. We have identified the surface ectoenzyme Vanin-2(VNN2) protein as a novel biomarke ...
DOI:
10.1016/j.molimm.2021.12.011

7. Humoral and cellular immune memory response 12 years following single dose vaccination against hepatitis A in Argentinian children

Author:
A. Urueña; M.N. Badano; P. Baré; J. González; R. Vicentín; R. Calli; M.C. Cañero-Velasco; S. Fink; C. Vizzotti
Source:
Vaccine 2022 v.40 no.1 pp. 114-121
ISSN:
0264-410X
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; Hepatovirus A; antibodies; antigens; flow cytometry; hepatitis A; immunologic memory; memory; vaccination; vaccines; Argentina
Abstract:
... Infants’ universal hepatitis A virus (HAV) single-dose vaccination has been highly effective for controlling HAV infection in Argentina, and in other Latin-American countries that adopted that strategy. Although antibodies wane over time, this has not been associated with HAV outbreaks or breakthrough infections, suggesting a relevant role for memory immunity. This study assessed long term humoral ...
DOI:
10.1016/j.vaccine.2021.11.037

8. Icaritin inhibited cigarette smoke extract-induced CD8+ T cell chemotaxis enhancement by targeting the CXCL10/CXCR3 axis and TGF-β/Smad2 signaling

Author:
Qiuping Li; Jing Sun; Yuxue Cao; Baojun Liu; Zhengxiao Zhao; Lingli Hu; Hu Zhang; Qing Kong; Jinfeng Wu; Jingcheng Dong
Source:
Phytomedicine 2022 v.96 pp. 153907
ISSN:
0944-7113
Subject:
CD8-positive T-lymphocytes; RNA; apoptosis; chemotaxis; cigarettes; drugs; lung function; lungs; macrophages; models; respiratory tract diseases; secretion; sequence analysis; smoke; therapeutics
Abstract:
... Chronic obstructive pulmonary disease (COPD) is a disabling/fatal disease characterized by progressive pulmonary function decline, and there are currently few drugs that can effectively reverse the decline in lung function; therefore, it is necessary to find novel drug targets. CD8⁺ T cells might be a new therapeutic target for alleviating lung tissue destruction and improving pulmonary function i ...
DOI:
10.1016/j.phymed.2021.153907

9. Magnesium sensing via LFA-1 regulates CD8+ T cell effector function

Author:
Jonas Lötscher; Adrià-Arnau Martí i Líndez; Nicole Kirchhammer; Elisabetta Cribioli; Greta Maria Paola Giordano Attianese; Marcel P. Trefny; Markus Lenz; Sacha I. Rothschild; Paolo Strati; Marco Künzli; Claudia Lotter; Susanne H. Schenk; Philippe Dehio; Jordan Löliger; Ludivine Litzler; David Schreiner; Victoria Koch; Nicolas Page; Dahye Lee; Jasmin Grählert; Dmitry Kuzmin; Anne-Valérie Burgener; Doron Merkler; Miklos Pless; Maria L. Balmer; Walter Reith; Jörg Huwyler; Melita Irving; Carolyn G. King; Alfred Zippelius; Christoph Hess
Source:
Cell 2022 v.185 no.4 pp. 585-602.e29
ISSN:
0092-8674
Subject:
CD8-positive T-lymphocytes; blood serum; calcium; cell-mediated immunity; cytotoxicity; disease progression; magnesium; signal transduction; synapse
Abstract:
... The relevance of extracellular magnesium in cellular immunity remains largely unknown. Here, we show that the co-stimulatory cell-surface molecule LFA-1 requires magnesium to adopt its active conformation on CD8⁺ T cells, thereby augmenting calcium flux, signal transduction, metabolic reprogramming, immune synapse formation, and, as a consequence, specific cytotoxicity. Accordingly, magnesium-suff ...
DOI:
10.1016/j.cell.2021.12.039

10. Sialic acid blockade in dendritic cells enhances CD8+ T cell responses by facilitating high-avidity interactions

Author:
N. Balneger; L. A. M. Cornelissen; M. Wassink; S. J. Moons; T. J. Boltje; Y. E. Bar-Ephraim; K. K. Das; J. N. Søndergaard; C. Büll; G. J. Adema
Source:
Cellular and molecular life sciences 2022 v.79 no.2 pp. 98
ISSN:
1420-682X
Subject:
CD8-positive T-lymphocytes; Toll-like receptors; cell adhesion; cell proliferation; gene expression; gene expression regulation; glycolipids; glycoproteins; mice; polysaccharides; sialic acid; viability
Abstract:
... Sialic acids are negatively charged carbohydrates that cap the glycans of glycoproteins and glycolipids. Sialic acids are involved in various biological processes including cell–cell adhesion and immune recognition. In dendritic cells (DCs), the major antigen-presenting cells of the immune system, sialic acids emerge as important regulators of maturation and interaction with other lymphocytes incl ...
DOI:
10.1007/s00018-021-04027-x

11. Spotlight on TAP and its vital role in antigen presentation and cross-presentation

Author:
Ian Mantel; Barzan A. Sadiq; J. Magarian Blander
Source:
Molecular immunology 2022 v.142 pp. 105-119
ISSN:
0161-5890
Subject:
CD8-positive T-lymphocytes; antigen presentation; antigens; cytotoxicity; endoplasmic reticulum; endosomes; major histocompatibility complex; peptides; phagosomes; therapeutics
Abstract:
... In the late 1980s and early 1990s, the hunt for a transporter molecule ostensibly responsible for the translocation of peptides across the endoplasmic reticulum (ER) membrane yielded the successful discovery of transporter associated with antigen processing (TAP) protein. TAP is a heterodimer complex comprised of TAP1 and TAP2, which utilizes ATP to transport cytosolic peptides into the ER across ...
DOI:
10.1016/j.molimm.2021.12.013

12. TIL expansion with high dose IL-2 or low dose IL-2 with anti-CD3/anti-CD28 stimulation provides different quality of TIL-expanded T cell clones

Author:
Thidarat Kongkaew; Rattapoom Thaiwong; Suparat Tudsamran; Thitiya Sae-jung; Panjana Sengprasert; Apichai Vasuratna; Koramit Suppipat; Rangsima Reantragoon
Source:
Journal of immunological methods 2022 v.503 pp. 113229
ISSN:
0022-1759
Subject:
CD8-positive T-lymphocytes; immunotherapy; interleukin-2; ovarian neoplasms
Abstract:
... Tumor infiltrating lymphocytes (TILs) are cells that are present inside the tumor environment, of which include T cells, B cells and natural killer (NK) cells. At present, TILs are used for immunotherapy in various cancers. Knowledge on adoptive transfer of TILs in ovarian cancer is still limited, especially regarding TIL expansion methods. Therefore, the aim of our study was to compare the qualit ...
DOI:
10.1016/j.jim.2022.113229

13. The enhanced cell cycle related to the response to adjuvant therapy in pancreatic ductal adenocarcinoma

Author:
Yize Mao; Weisheng Cheng; Qiuxia Yang; Liqin Li; Wanming Hu; Zeyu Shuang; Dejun Fan; Xinlei Jiang; Feng Gao; Shengping Li; Wei Wang
Source:
Genomics 2022 v.114 no.1 pp. 95-106
ISSN:
0888-7543
Subject:
CD8-positive T-lymphocytes; adenocarcinoma; adjuvants; biomarkers; cell cycle; drug therapy; gene expression regulation; genomics; hypoxia; macrophages; patients; statistical analysis; suppressor genes
Abstract:
... A major clinical challenge for treating patients with pancreatic ductal adenocarcinoma (PDAC) is identifying those that may benefit from adjuvant chemotherapy versus those that will not. Thus, there is a need for a robust and convenient biomarker for predicting chemotherapy response in PDAC patients. In this study, network inference was conducted by integrating the differentially expressed cell cy ...
DOI:
10.1016/j.ygeno.2021.11.036

14. A novel epithelial-mesenchymal transition-related gene signature for prognosis prediction in patients with lung adenocarcinoma

Author:
Shengyu Feng; Ce Huang; Liuling Guo; Hao Wang; Hailiang Liu
Source:
Heliyon 2022 v.8 no.1 pp. e08713
ISSN:
2405-8440
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; Singapore; adenocarcinoma; cell movement; data collection; genes; immunity; immunotherapy; lungs; microarray technology; models; neoplasm cells; prediction; prognosis; regression analysis; risk; sequence analysis
Abstract:
... Traditional pathological diagnoses and clinical methods are insufficient to accurately predict the prognosis of lung adenocarcinoma (LUAD). Epithelial-mesenchymal transition (EMT) process is closely related to tumor cell migration. However, the prognostic value of EMT-related genes in LUAD is still unclear. In this study, we collected bulk RNA-sequencing (RNA-seq) and microarray data of LUAD patie ...
DOI:
10.1016/j.heliyon.2022.e08713

15. A novel self-assembled epitope peptide nanoemulsion vaccine targeting nasal mucosal epithelial cell for reinvigorating CD8+ T cell immune activity and inhibiting tumor progression

Author:
Yun Yang; Shuang Ge; Zhen Song; Anni Zhao; Liqun Zhao; Zhiming Hu; Dingyi Cai; Zelong Zhang; Liusheng Peng; Dongshui Lu; Ping Luo; Weijun Zhang; Hongwu Sun; Quanming Zou; Hao Zeng
Source:
International journal of biological macromolecules 2021 v.183 pp. 1891-1902
ISSN:
0141-8130
Subject:
CD8-positive T-lymphocytes; antibodies; cancer vaccines; encapsulation; epithelial cells; epitopes; immune response; immunogenicity; integrins; intranasal administration; laminin; lungs; mucins; nanoemulsions; nasal mucosa; neoplasm progression; particle size; peptides; vaccine development; zeta potential
Abstract:
... Epitope peptides are not suitable for nasal administration immunity due to their poor immunogenicity and low delivery efficiency. Here, we reported an intranasal self-assembled nanovaccine (I-OVA NE), which was loaded with the peptides IKVAV-OVA₂₅₇–₂₆₄ (I-OVA), a laminin peptide (Ile-Lys-Val-ala-Val, IKVAV) and OVA₂₅₇–₂₆₄ epitope conjugated peptide. This nanovaccine with I-OVA at a concentration o ...
DOI:
10.1016/j.ijbiomac.2021.05.158

16. Adaptive Immune Responses, Immune Escape and Immune-Mediated Pathogenesis during HDV Infection

Author:
Valerie Oberhardt; Maike Hofmann; Robert Thimme; Christoph Neumann-Haefelin
Source:
Viruses 2022 v.14 no.2 pp. -
ISSN:
1999-4915
Subject:
CD8-positive T-lymphocytes; HLA antigens; Hepatitis B virus; Hepatitis C virus; Hepatitis delta virus; Human immunodeficiency virus; adaptive immunity; humans; interferons; liver cirrhosis; mixed infection; pathogenesis; satellite viruses; vertebrate viruses
Abstract:
... The hepatitis delta virus (HDV) is the smallest known human virus, yet it causes great harm to patients co-infected with hepatitis B virus (HBV). As a satellite virus of HBV, HDV requires the surface antigen of HBV (HBsAg) for sufficient viral packaging and spread. The special circumstance of co-infection, albeit only one partner depends on the other, raises many virological, immunological, and pa ...
DOI:
10.3390/v14020198

17. Antigen dominance hierarchies shape TCF1+ progenitor CD8 T cell phenotypes in tumors

Author:
Megan L. Burger; Amanda M. Cruz; Grace E. Crossland; Giorgio Gaglia; Cecily C. Ritch; Sarah E. Blatt; Arjun Bhutkar; David Canner; Tamina Kienka; Sara Z. Tavana; Alexia L. Barandiaran; Andrea Garmilla; Jason M. Schenkel; Michelle Hillman; Izumi de los Rios Kobara; Amy Li; Alex M. Jaeger; William L. Hwang; Peter M.K. Westcott; Michael P. Manos; Marta M. Holovatska; F. Stephen Hodi; Aviv Regev; Sandro Santagata; Tyler Jacks
Source:
Cell 2021 v.184 no.19 pp. 4996-5014.e26
ISSN:
0092-8674
Subject:
CD8-positive T-lymphocytes; adenocarcinoma; antigens; data collection; humans; lungs; mice; phenotype; vaccination
Abstract:
... CD8 T cell responses against different tumor neoantigens occur simultaneously, yet little is known about the interplay between responses and its impact on T cell function and tumor control. In mouse lung adenocarcinoma, we found that immunodominance is established in tumors, wherein CD8 T cell expansion is predominantly driven by the antigen that most stably binds MHC. T cells responding to subdom ...
DOI:
10.1016/j.cell.2021.08.020

18. Antitumor Efficacy of MUC1-derived Variable Epitope Library Treatments in a Mouse Model of Breast Cancer

Author:
Josue Odales; Rodolfo Servin-Blanco; Fernando Martinez-Cortes; Jesus Guzman Valle; Allan Noe Dominguez-Romero; Goar Gevorkian; Karen Manoutcharian
Source:
Vaccine 2022 pp. -
ISSN:
0264-410X
Subject:
CD8-positive T-lymphocytes; breast neoplasms; epitopes; humans; immune response; lungs; metastasis; mice; mutants; peptides; prognosis; signal peptide; tandem repeat sequences; vaccination; vaccine development; vaccines
Abstract:
... The identification of novel targets for cancer immunotherapy and the development of new vaccine immunogens are subjects of permanent interest. MUC1 is an overexpressed antigen found in most tumors, and its overexpression correlates with poor prognosis. Many attempts to direct the immune response against MUC1 in tumor cells have failed, including several clinical trials. We have previously develope ...
DOI:
10.1016/j.vaccine.2022.06.062

19. B-Cell Control of Regulatory T Cells in Friend Virus Infection

Author:
Tyler C. Moore; Kim J. Hasenkrug
Source:
Journal of molecular biology 2021 v.433 no.1 pp. 166583
ISSN:
0022-2836
Subject:
B-lymphocytes; CD8-positive T-lymphocytes; antibodies; antibody formation; antigen-presenting cells; models
Abstract:
... B lymphocytes have well-established effector roles during viral infections, including production of antibodies and functioning as antigen-presenting cells for CD4+ and CD8+ T cells. B cells have also been shown to regulate immune responses and induce regulatory T cells (Tregs). In the Friend virus (FV) model, Tregs are known to inhibit effector CD8+ T-cell responses and contribute to virus persist ...
DOI:
10.1016/j.jmb.2020.06.022

20. Bag it, tag it: ubiquitin ligases and host resistance to Mycobacterium tuberculosis

Author:
Priscila C. Campos; Danielle T. Cunha; Luiz P. Souza-Costa; Michael U. Shiloh; Luis H. Franco
Source:
Trends in microbiology 2022 pp. -
ISSN:
0966-842X
Subject:
CD8-positive T-lymphocytes; Mycobacterium tuberculosis; anti-infective agents; apoptosis; autophagy; cytokines; enzyme activity; etiological agents; inflammation; lipids; microbiology; phagocytosis; phagosomes; tuberculosis; ubiquitin; ubiquitin-protein ligase; ubiquitination
Abstract:
... Infection with Mycobacterium tuberculosis (Mtb), the etiological agent of tuberculosis (TB), remains a significant global epidemic. Host resistance to Mtb depends on both adaptive and innate immunity mechanisms, including development of antigen-specific CD4 and CD8 T cells, production of inflammatory cytokines, bacterial phagocytosis and destruction within phagolysosomes, host cell apoptosis, and ...
DOI:
10.1016/j.tim.2022.03.010

21. CD4+ and CD8+ T-cell responses in bone marrow to fatty acids in high-fat diets

Author:
Mayte Medrano; Ana Lemus-Conejo; Sergio Lopez; Maria C. Millan-Linares; Maria A. Rosillo; Manuel Muñiz; Rocio Calderon; Sara Jaramillo; Jose A. Perez-Simon; Francisco J.G. Muriana; Rocio Abia
Source:
Journal of nutritional biochemistry 2022 v.107 pp. 109057
ISSN:
0955-2863
Subject:
CD8-positive T-lymphocytes; adaptive immunity; bone marrow; docosahexaenoic acid; eicosapentaenoic acid; obesity; oleic acid; spleen
Abstract:
... Obesity is associated with disruptions in the adaptive immune system; however, dietary fatty acids in high-fat diets (HFDs) that induce obesity have consequences that are currently unclear regarding T-cell maintenance in bone marrow (BM). C57BL/6J mice were randomly assigned to isocaloric HFDs formulated with dietary fats rich in saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), o ...
DOI:
10.1016/j.jnutbio.2022.109057

22. Complete genome sequencing and evolutionary analysis of hepatitis C virus subtype 6a, including strains from Guangdong Province, China

Author:
Ru Xu; Hao Wang; Jieting Huang; Min Wang; Qiao Liao; Zhengang Shan; Huishan Zhong; Xia Rong; Yongshui Fu
Source:
Archives of virology 2022 v.167 no.2 pp. 591-596
ISSN:
0304-8608
Subject:
CD8-positive T-lymphocytes; Hepatitis C virus; epitopes; genome; nucleotide sequences; virology; viruses; China; Vietnam
Abstract:
... We performed an evolutionary analysis using whole genome sequence isolates of hepatitis C virus (HCV) 6a from Guangdong Province and reference sequences from various countries. Less than 5% of the HCV genome was found to be under positive selection. The E1 and E2 proteins had the highest proportion of positively selected sites both within and outside of CD8 T cell epitopes in all of the strains. R ...
DOI:
10.1007/s00705-021-05358-7

23. Development of a long-term, IL7 dependent cell death rescue assay in CD4+ T-cells

Author:
Daniel Perez-Witzke; Rekha Parmar; Ileana Blancq; Marie-Alix Turpin; Frederique Ponchel
Source:
Journal of immunological methods 2021 v.493 pp. 113020
ISSN:
0022-1759
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; agonists; antagonism; antibodies; cell cycle; cell death; cell proliferation; cell viability; drugs; dyes; interleukin-7; long term effects; mitogens; phytohemagglutinin; therapeutics
Abstract:
... Interfering with signalling pathways by targeting cell surface proteins has become an important strategy in the development of novel therapeutic agents. Notably, interfering with cytokine signalling revolutionised the treatment of chronic diseases. Cytokines can induce a range of effects that are not always accounted for in assays detecting cytokine binding to cell surface receptors and/or proxima ...
DOI:
10.1016/j.jim.2021.113020

24. Development of a novel ZIKV vaccine comprised of immunodominant CD4+ and CD8+ T cell epitopes identified through comprehensive epitope mapping in Zika virus infected mice

Author:
Jin Sun; Zhihang Zheng; Min Li; Zhihua Liu; Xiao Su; Xia Jin
Source:
Vaccine 2021 v.39 no.36 pp. 5173-5186
ISSN:
0264-410X
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; Zika virus; antibodies; cytokines; cytotoxicity; humans; immunization; immunodominant epitopes; polyproteins; vaccines; viruses
Abstract:
... Zika virus (ZIKV) caused over two million human infections in more than 80 countries around 2015–2016. Current vaccines under development are mostly focused on inducing antibodies that despite capable of inhibiting the virus, may have the potential to trigger antibody dependent enhancement (ADE). T cell vaccines that do not induce antibodies targeting viral surface will unlikely cause ADE, but be ...
DOI:
10.1016/j.vaccine.2021.07.036

25. Distinct Plasma Concentrations of Acyl-CoA-Binding Protein (ACBP) in HIV Progressors and Elite Controllers

Author:
Stéphane Isnard; Léna Royston; John Lin; Brandon Fombuena; Simeng Bu; Sanket Kant; Tsoarello Mabanga; Carolina Berini; Mohamed El-Far; Madeleine Durand; Cécile L. Tremblay; Nicole F. Bernard; Guido Kroemer; Jean-Pierre Routy
Source:
Viruses 2022 v.14 no.3 pp. -
ISSN:
1999-4915
Subject:
CD8-positive T-lymphocytes; acyl coenzyme A; antiretroviral agents; autophagy; interleukin-6; interleukin-8; people; therapeutics; virus replication
Abstract:
... HIV elite controllers (ECs) are characterized by the spontaneous control of viral replication, and by metabolic and autophagic profiles which favor anti-HIV CD4 and CD8 T-cell responses. Extracellular acyl coenzyme A binding protein (ACBP) acts as a feedback inhibitor of autophagy. Herein, we assessed the circulating ACBP levels in ECs, compared to people living with HIV (PLWH) receiving antiretro ...
DOI:
10.3390/v14030453

26. Dual role of immunomodulation by crude polysaccharide from okra against carcinogenic liver injury in mice

Author:
Suhailah Hayaza; Sri Puji Astuti Wahyuningsih; Raden Joko Kuncoroningrat Susilo; Saikhu Akhmad Husen; Dwi Winarni; Ruey-an Doong; Win Darmanto
Source:
Heliyon 2021 v.7 no.2 pp. e06183
ISSN:
2405-8440
Subject:
Abelmoschus esculentus; CD8-positive T-lymphocytes; body weight; carcinogenicity; diethylnitrosamine; doxorubicin; immunomodulation; interleukin-2; liver; macrophages; males; okra; polysaccharides; Indonesia
Abstract:
... Polysaccharides have long been recognized as the anticancer agent with low toxicity and slight side effects. Okra (Abelmoschus esculentus L.), a flowering plant from the Malvaceae family that is found in tropical, subtropical, and warm temperate regions around the world. Hence, no in vivo studies have addressed the anticancer and immunomodulatory potential of polysaccharides from okra pods grown i ...
DOI:
10.1016/j.heliyon.2021.e06183

27. ELISA based on peptide antigens reproducing cross-reactive viral epitopes to detect antibodies in latent autoimmune diabetes in adults vs. type 1 diabetes

Author:
Feliciana Real-Fernández; Alessandra Gallo; Francesca Nuti; Lorenzo Altamore; Gloria Giovanna Del Vescovo; Pietro Traldi; Eugenio Ragazzi; Paolo Rovero; Annunziata Lapolla; Anna Maria Papini
Source:
MethodsX 2021 v.8 pp. 101452
ISSN:
2215-0161
Subject:
CD8-positive T-lymphocytes; autoantibodies; autoimmunity; biomarkers; diagnostic techniques; disease diagnosis; epitopes; insulin-dependent diabetes mellitus; patients; synthetic peptides
Abstract:
... Diagnosis of Latent Autoimmune Diabetes in Adults (LADA) is based on the adult-age, anti-islet autoantibodies, and temporary insulin-independence. As in Type-1-Diabetes (T1DM), autoimmunity may trigger LADA and enteroviruses-infections can play a role. Anti-human Glutamic-Acid-Decarboxylase (hGAD) autoantibodies are accepted clinical biomarkers, but do not discriminate LADA vs. T1DM. The hypothesi ...
DOI:
10.1016/j.mex.2021.101452

28. Eomes and T‐bet, a dynamic duo regulating NK cell differentiation

Author:
Jiang Zhang; Noémi Rousseaux; Thierry Walzer
Source:
BioEssays 2022 v.44 no.3 pp. e2100281
ISSN:
0265-9247
Subject:
CD8-positive T-lymphocytes; DNA; cell differentiation; chromatin; cytotoxicity; epigenetics; gene expression; transcription (genetics)
Abstract:
... T‐bet and Eomes are two related transcription factors (TFs) that regulate the differentiation of cytotoxic lymphocytes such as Natural Killer (NK) cells and CD8 T cells. Recent genome‐wide analyses suggest they have complementary roles in instructing the transcriptional program of NK cells, although their DNA binding sites appear to be very similar. In this essay, we discuss the mechanisms that co ...
DOI:
10.1002/bies.202100281

29. Expression of Id3 represses exhaustion of anti-tumor CD8 T cells in liver cancer

Author:
Yun Jin; Pingping Hu; Haihang Sun; Chao Yang; Jianxin Zhai; Yi Wang; Xinyun Chu; Zhiwei Sun; Jia Wang; Jie Sun; Junfeng Wang
Source:
Molecular immunology 2022 v.144 pp. 117-126
ISSN:
0161-5890
Subject:
CD8-positive T-lymphocytes; DNA; homeostasis; immunology; liver neoplasms; memory
Abstract:
... Id3, an inhibitor of DNA binding protein, plays important roles in the function and homeostasis of effector and memory T cells. Recent evidence has shown that Id3 is also implicated in CD8 T cell exhaustion. However, whether and how Id3 might regulate effector function or exhaustion of CD8 T cells, especially in the tumor setting, is still unknown. Here, we first showed that Id3 expression was imp ...
DOI:
10.1016/j.molimm.2022.02.005

30. Germinal center responses to SARS-CoV-2 mRNA vaccines in healthy and immunocompromised individuals

Author:
Katlyn Lederer; Emily Bettini; Kalpana Parvathaneni; Mark M. Painter; Divyansh Agarwal; Kendall A. Lundgreen; Madison Weirick; Kavitha Muralidharan; Diana Castaño; Rishi R. Goel; Xiaoming Xu; Elizabeth M. Drapeau; Sigrid Gouma; Jordan T. Ort; Moses Awofolaju; Allison R. Greenplate; Carole Le Coz; Neil Romberg; Jennifer Trofe-Clark; Gregory Malat; Lisa Jones; Mark Rosen; Daniela Weiskopf; Alessandro Sette; Behdad Besharatian; Mary Kaminiski; Scott E. Hensley; Paul Bates; E. John Wherry; Ali Naji; Vijay Bhoj; Michela Locci
Source:
Cell 2022 v.185 no.6 pp. 1008-1024.e15
ISSN:
0092-8674
Subject:
B-lymphocytes; CD8-positive T-lymphocytes; Severe acute respiratory syndrome coronavirus 2; humans; kidney transplant; lymph; lymph nodes; memory; vaccination
Abstract:
... Vaccine-mediated immunity often relies on the generation of protective antibodies and memory B cells, which commonly stem from germinal center (GC) reactions. An in-depth comparison of the GC responses elicited by SARS-CoV-2 mRNA vaccines in healthy and immunocompromised individuals has not yet been performed due to the challenge of directly probing human lymph nodes. Herein, through a fine-needle ...
DOI:
10.1016/j.cell.2022.01.027

31. Hierarchy of multiple viral CD8+ T-cell epitope mutations in sequential selection in simian immunodeficiency infection

Author:
Nana Afia Asante Ntim; Hiroshi Ishii; Moe Jomori; Hiroyuki Yamamoto; Tetsuro Matano; Takushi Nomura
Source:
Biochemical and biophysical research communications 2022 v.607 pp. 124-130
ISSN:
0006-291X
Subject:
CD8-positive T-lymphocytes; HIV infections; Simian immunodeficiency virus; disease progression; early selection; epitopes; haplotypes; immunosuppression; major histocompatibility complex; models; mutation; research; virus replication
Abstract:
... CD8⁺ T-cell responses exert strong suppressive pressure on viral replication and select for viral escape mutations in HIV infection. Multiple viral epitopes restricted by major histocompatibility complex class I (MHC-I) are targeted by CD8⁺ T cells. Sequential selection of viral escape mutations in individual epitope-coding regions could result in failure in CD8⁺ T cell-based viral control leading ...
DOI:
10.1016/j.bbrc.2022.03.108

32. High CD169 Monocyte/Lymphocyte Ratio Reflects Immunophenotype Disruption and Oxygen Need in COVID-19 Patients

Author:
Antonella Minutolo; Vita Petrone; Marialaura Fanelli; Marco Iannetta; Martina Giudice; Ines Ait Belkacem; Marta Zordan; Pietro Vitale; Guido Rasi; Paola Sinibaldi-Vallebona; Loredana Sarmati; Massimo Andreoni; Fabrice Malergue; Emanuela Balestrieri; Sandro Grelli; Claudia Matteucci
Source:
Pathogens 2021 v.10 no.12 pp. -
ISSN:
2076-0817
Subject:
B-lymphocytes; CD8-positive T-lymphocytes; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; biomarkers; disease progression; dose response; flow cytometry; fluorescence; immunophenotype; immunophenotyping; interleukin-10; interleukin-6; lectins; monocytes; oxygen; pneumonia; transcription (genetics)
Abstract:
... Background: Sialoadhesin (CD169) has been found to be overexpressed in the blood of COVID-19 patients and identified as a biomarker in early disease. We analyzed CD169 in the blood cells of COVID-19 patients to assess its role as a predictive marker of disease progression and clinical outcomes. Methods: The ratio of the median fluorescence intensity of CD169 between monocytes and lymphocytes (CD16 ...
DOI:
10.3390/pathogens10121639

33. Identification of Novel Candidate CD8+ T Cell Epitopes of the SARS-CoV2 with Homology to Other Seasonal Coronaviruses

Author:
Pradeep Darshana Pushpakumara; Deshan Madhusanka; Saubhagya Dhanasekara; Chandima Jeewandara; Graham S. Ogg; Gathsaurie Neelika Malavige
Source:
Viruses 2021 v.13 no.6 pp. -
ISSN:
1999-4915
Subject:
CD8-positive T-lymphocytes; Orthocoronavirinae; databases; epitopes; immunity; immunopathology; nucleocapsid; pathogenesis; phenotype; viruses
Abstract:
... Cross-reactive T cell immunity to seasonal coronaviruses (HCoVs) may lead to immunopathology or protection during SARS-CoV2 infection. To understand the influence of cross-reactive T cell responses, we used IEDB (Immune epitope database) and NetMHCpan (ver. 4.1) to identify candidate CD8⁺ T cell epitopes, restricted through HLA-A and B alleles. Conservation analysis was carried out for these epito ...
DOI:
10.3390/v13060972

34. Identification of promising CD8 and CD4 T cell epitopes for peptide vaccine formulation against SARS-CoV-2

Author:
Supriyo Chakraborty; Bornali Deb; Durbba Nath; Deboja Monoswita
Source:
Archives of microbiology 2022 v.204 no.5 pp. 242
ISSN:
0302-8933
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; adjuvants; amino acids; cost effectiveness; epitopes; glycoproteins; glycosylation; humans; hydrophilicity; immunogenicity; immunoinformatics; membrane glycoproteins; pandemic; peptides; viruses
Abstract:
... The novel virus “severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)” has been responsible for the worldwide pandemic causing huge devastation and deaths since December 2019. The disease caused by this virus is known as COVID-19. The present study is based on immuno-informatics approach to develop a multi-epitope-loaded peptide vaccine to combat the COVID-19 menace. Here, we have reported ...
DOI:
10.1007/s00203-022-02845-6

35. Immunogenic epitope prediction to create a universal influenza vaccine

Author:
R.R. Mintaev; D.V. Glazkova; E.V. Bogoslovskaya; G.A. Shipulin
Source:
Heliyon 2022 v.8 no.5 pp. e09364
ISSN:
2405-8440
Subject:
B-lymphocytes; CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; Orthomyxoviridae; epitopes; humans; immunity; influenza; influenza vaccines; morbidity; mortality; prediction; viruses
Abstract:
... Influenza virus is one of the most rapidly evolving human pathogens and causes significant morbidity and mortality worldwide. This feature enables the virus to avoid natural or vaccine-induced immunity. For this reason, there is an intensive search for new approaches to create a universal influenza vaccine. Here, we propose pipelines based on modern prediction algorithms that allowed us to select ...
DOI:
10.1016/j.heliyon.2022.e09364

36. Immunoinformatics approach for multi-epitope vaccine design against P. falciparum malaria

Author:
Leah Maharaj; Victoria T. Adeleke; Abiodun J. Fatoba; Adebayo A. Adeniyi; Selaelo I. Tshilwane; Matthew A. Adeleke; Rajendra Maharaj; Moses Okpeku
Source:
Infection, genetics, and evolution 2021 v.92 pp. 104875
ISSN:
1567-1348
Subject:
B-lymphocytes; CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; HLA antigens; Plasmodium falciparum; adjuvants; computer simulation; epitopes; etiological agents; evolution; falciparum malaria; genetics; immune response; immunogenicity; immunoinformatics; infection; infectious diseases; molecular dynamics; prediction; vaccine development; vaccines
Abstract:
... Plasmodium falciparum (P. falciparum) is a leading causative agent of malaria, an infectious disease that can be fatal. Unfortunately, control measures are becoming less effective over time. A vaccine is needed to effectively control malaria and lead towards the total elimination of the disease. There have been multiple attempts to develop a vaccine, but to date, none have been certified as approp ...
DOI:
10.1016/j.meegid.2021.104875

37. Immunoinformatics prediction of overlapping CD8+ T-cell, IFN-γ and IL-4 inducer CD4+ T-cell and linear B-cell epitopes based vaccines against COVID-19 (SARS-CoV-2)

Author:
Abiodun J. Fatoba; Leah Maharaj; Victoria T. Adeleke; Moses Okpeku; Adebayo A Adeniyi; Matthew A. Adeleke
Source:
Vaccine 2021 v.39 no.7 pp. 1111-1121
ISSN:
0264-410X
Subject:
B-lymphocytes; CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; COVID-19 infection; HLA antigens; Severe acute respiratory syndrome coronavirus 2; Toll-like receptor 3; databases; epitopes; experimental design; glycoproteins; immunogenicity; immunoinformatics; interleukin-4; live vaccines; major histocompatibility complex; pandemic; prediction; structural proteins; vaccine development; viruses
Abstract:
... At the beginning of the year 2020, the world was struck with a global pandemic virus referred to as SARS-CoV-2 (COVID-19) which has left hundreds of thousands of people dead. To control this virus, vaccine design becomes imperative. In this study, potential epitopes-based vaccine candidates were explored. Six hundred (600) genomes of SARS-CoV-2 were retrieved from the viPR database to generate CD8 ...
DOI:
10.1016/j.vaccine.2021.01.003

38. Immunoinformatics-based characterization of immunogenic CD8 T-cell epitopes for a broad-spectrum cell-mediated immunity against high-risk human papillomavirus infection

Author:
Alexandre Santos Matos; Fernanda Oliveira Prado; Paloma Aparecida Santos Rocha; Marcus Vinicius Aragão Batista
Source:
Microbial pathogenesis 2022 v.165 pp. 105462
ISSN:
0882-4010
Subject:
CD8-positive T-lymphocytes; Papillomaviridae; cell-mediated immunity; energy; epitopes; humans; hydrogen; immunogenicity; pathogenesis; pathogens; toxicity; vaccination; vaccines
Abstract:
... Human papillomavirus (HPV) represents the most prevalent sexually transmitted infectious agent worldwide. Vaccination has been an approach successfully used as a prophylactic measure against this infectious agent in patients without previous contact with the genotypes present in the vaccine. In this work, we use a computational approach to predict CD8 T cell epitopes from HPV proteins to promote c ...
DOI:
10.1016/j.micpath.2022.105462

39. Impairment of CD4+ T and Memory B Cell Responses but Normal Memory CD8+T-Cell Activation on Crohn’s Disease after COVID-19 Vaccination: A Twin Case

Author:
Fabiana Gil Melgaço; Tamiris Azamor; Livia Melo Villar; Ana Paula Dinis Ano Bom; Juliana Gil Melgaço
Source:
Viruses 2021 v.13 no.11 pp. -
ISSN:
1999-4915
Subject:
B-lymphocytes; CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; antibody detection; antibody formation; autoimmune diseases; memory; patients; vaccination
Abstract:
... Vaccines to prevent the impact of SARS-CoV-2 are now available, including for patients with autoimmune diseases. However, there is no information about how inflammatory bowel disease (IBD) treatment could impact the cellular and humoral immune responses. This study evaluated SARS-CoV-2-specific humoral and cellular responses after vaccination with a two-dose schedule in a Crohn’s disease patient t ...
DOI:
10.3390/v13112143

40. In silico analysis of mutant epitopes in new SARS-CoV-2 lineages suggest global enhanced CD8+ T cell reactivity and also signs of immune response escape

Author:
Marco Antônio M. Pretti; Rômulo G. Galvani; Nicole M. Scherer; Alessandro S. Farias; Mariana Boroni
Source:
Infection, genetics, and evolution 2022 v.99 pp. 105236
ISSN:
1567-1348
Subject:
CD8-positive T-lymphocytes; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; antibodies; computer simulation; epitopes; evolution; immune response; infection; mutants; nucleocapsid; peptides; viral load; Brazil; South Africa
Abstract:
... SARS-CoV-2 variants of concern have emerged since the COVID-19 outburst, notably the lineages detected in the UK, South Africa, and Brazil. Their increased transmissibility and higher viral load put them in the spotlight. Much has been investigated on the ability of those new variants to evade antibody recognition. However, little attention has been given to pre-existing and induced SARS-CoV-2-spe ...
DOI:
10.1016/j.meegid.2022.105236

41. Increased angiopoietin-like 4 expression ameliorates inflammatory bowel diseases via suppressing CD8+ T cell activities

Author:
Xiaoyuan Zhang; Jing Tu; Shizhen Ding; Mei Wang; Yanbing Ding; Zhijie Lin; Guotao Lu; Weiming Xiao; Weijuan Gong
Source:
Biochemical and biophysical research communications 2022 v.612 pp. 37-43
ISSN:
0006-291X
Subject:
CD8-positive T-lymphocytes; Crohn disease; atherosclerosis; dextran sulfate; macrophages; noninsulin-dependent diabetes mellitus; pathogenesis; protective effect; research; respiratory tract diseases; rheumatoid arthritis; secretion; ulcerative colitis
Abstract:
... Angiopoietin-like 4 (ANGPTL4) is involved in inflammation-associated diseases, such as rheumatoid arthritis, type 2 diabetes, atherosclerosis, and chronic obstructive pulmonary disease. The role of ANGPTL4 in the pathogenesis of inflammatory bowel disease (IBD) remains unknown. Here, the plasma ANGPTL4 levels peaked on days 3 and 5, and expression of ANGPTL4 of inflamed colons peaked on days 5 and ...
DOI:
10.1016/j.bbrc.2022.03.153

42. Manipulating immune system using nanoparticles for an effective cancer treatment: Combination of targeted therapy and checkpoint blockage miRNA

Author:
Hanh Thuy Nguyen; Cao Dai Phung; Tuan Hiep Tran; Tung Thanh Pham; Le Minh Pham; Tiep Tien Nguyen; Jee-Heon Jeong; Han-Gon Choi; Sae Kwang Ku; Chul Soon Yong; Jong Oh Kim
Source:
Journal of controlled release 2021 v.329 pp. 524-537
ISSN:
0168-3659
Subject:
CD8-positive T-lymphocytes; antagonists; binding capacity; blood circulation; cell death; cytotoxicity; drugs; immune response; immunosuppression; lymph nodes; microRNA; nanoparticles; neoplasm cells; neoplasms; pH; patients; peptides; spleen; therapeutics
Abstract:
... Accumulating clinical data shows that less than half of patients are beneficial from PD-1/PD-L1 blockage therapy owing to the limited infiltration of effector immune cells into the tumor and abundant of the immunosuppressive factors in the tumor microenvironment. In this study, PD-L1 inhibition therapy and BRAF-targeted therapy, which showed clinical benefit, were combined in a CXCR4-targeted nano ...
DOI:
10.1016/j.jconrel.2020.09.034

43. Melanoma-associated fibroblasts impair CD8+ T cell function and modify expression of immune checkpoint regulators via increased arginase activity

Author:
Barbara Érsek; Pálma Silló; Ugur Cakir; Viktor Molnár; András Bencsik; Balázs Mayer; Eva Mezey; Sarolta Kárpáti; Zoltán Pós; Krisztián Németh
Source:
Cellular and molecular life sciences 2021 v.78 no.2 pp. 661-673
ISSN:
1420-682X
Subject:
CD8-positive T-lymphocytes; arginase; arginine; cytotoxicity; fibroblasts; flow cytometry; genetically modified organisms; ligands
Abstract:
... This study shows that melanoma-associated fibroblasts (MAFs) suppress cytotoxic T lymphocyte (CTL) activity and reveals a pivotal role played by arginase in this phenomenon. MAFs and normal dermal fibroblasts (DFs) were isolated from surgically resected melanomas and identified as Melan-A-/gp100-/FAP+ cells. CTLs of healthy blood donors were activated in the presence of MAF- and DF-conditioned med ...
DOI:
10.1007/s00018-020-03517-8

44. Memory CD8 T Cells Protect against Cytomegalovirus Disease by Formation of Nodular Inflammatory Foci Preventing Intra-Tissue Virus Spread

Author:
Rafaela Holtappels; Jürgen Podlech; Kirsten Freitag; Niels A. Lemmermann; Matthias J. Reddehase
Source:
Viruses 2022 v.14 no.6 pp. -
ISSN:
1999-4915
Subject:
CD8-positive T-lymphocytes; Cytomegalovirus; cell transplantation; histopathology; immunotherapy; mice; risk; viruses
Abstract:
... Cytomegaloviruses (CMVs) are controlled by innate and adaptive immune responses in an immunocompetent host while causing multiple organ diseases in an immunocompromised host. A risk group of high clinical relevance comprises transiently immunocompromised recipients of hematopoietic cell transplantation (HCT) in the “window of risk” between eradicative therapy of hematopoietic malignancies and comp ...
DOI:
10.3390/v14061145

45. Multi-Epitope Vaccine Design Using an Immunoinformatic Approach for SARS-CoV-2

Author:
Ye Feng; Haiping Jiang; Min Qiu; Liang Liu; Shengmei Zou; Yun Li; Qianpeng Guo; Ning Han; Yingqiang Sun; Kui Wang; Lantian Lu; Xinlei Zhuang; Shanshan Zhang; Shuqing Chen; Fan Mo
Source:
Pathogens 2021 v.10 no.6 pp. -
ISSN:
2076-0817
Subject:
B-lymphocytes; CD8-positive T-lymphocytes; COVID-19 infection; HLA antigens; Severe acute respiratory syndrome coronavirus 2; animal models; blood serum; computer simulation; epitopes; hydrogen; immunoinformatics; lymph; mutation rate; peptides; vaccine development; viral genome
Abstract:
... Through 4 June 2021, COVID-19 has caused over 172.84 million cases of infection and 3.71 million deaths worldwide. Due to its rapid dissemination and high mutation rate, it is essential to develop a vaccine harboring multiple epitopes and efficacious against multiple variants to prevent the immune escape of SARS-CoV-2. An in silico approach based on the viral genome was applied to identify 19 high ...
DOI:
10.3390/pathogens10060737

46. Neoantigen-driven B cell and CD4 T follicular helper cell collaboration promotes anti-tumor CD8 T cell responses

Author:
Can Cui; Jiawei Wang; Eric Fagerberg; Ping-Min Chen; Kelli A. Connolly; Martina Damo; Julie F. Cheung; Tianyang Mao; Adnan S. Askari; Shuting Chen; Brittany Fitzgerald; Gena G. Foster; Stephanie C. Eisenbarth; Hongyu Zhao; Joseph Craft; Nikhil S. Joshi
Source:
Cell 2021 v.184 no.25 pp. 6101-6118.e13
ISSN:
0092-8674
Subject:
B-lymphocytes; CD8-positive T-lymphocytes; adenocarcinoma; immunity; interleukins; lungs; lymph nodes; mice; models; transcription (genetics)
Abstract:
... CD4 T follicular helper (TFH) cells support B cells, which are critical for germinal center (GC) formation, but the importance of TFH-B cell interactions in cancer is unclear. We found enrichment of TFH cell transcriptional signature correlates with GC B cell signature and with prolonged survival in individuals with lung adenocarcinoma (LUAD). We further developed a murine LUAD model in which tumo ...
DOI:
10.1016/j.cell.2021.11.007

47. New Latency Reversing Agents for HIV-1 Cure: Insights from Nonhuman Primate Models

Author:
Katherine M. Bricker; Ann Chahroudi; Maud Mavigner
Source:
Viruses 2021 v.13 no.8 pp. -
ISSN:
1999-4915
Subject:
CD8-positive T-lymphocytes; Human immunodeficiency virus 1; Toll-like receptors; antiretroviral agents; caspases; disease progression; mitochondria; therapeutics
Abstract:
... Antiretroviral therapy (ART) controls human immunodeficiency virus 1 (HIV-1) replication and prevents disease progression but does not eradicate HIV-1. The persistence of a reservoir of latently infected cells represents the main barrier to a cure. “Shock and kill” is a promising strategy involving latency reversing agents (LRAs) to reactivate HIV-1 from latently infected cells, thus exposing the ...
DOI:
10.3390/v13081560

48. Normal weight obesity is associated with hypercholesterolemia and low muscle mass in persons living with HIV on antiretroviral treatment

Author:
Ivan A. Osuna-Padilla; Nadia C. Rodríguez-Moguel; Adriana Aguilar-Vargas; Edna Rodríguez-Aguirre; Olivia Briceño; Santiago Ávila-Ríos
Source:
Nutrition 2022 v.94 pp. 111515
ISSN:
0899-9007
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; adults; antiretroviral agents; blood glucose; blood pressure; body fat; body mass index; bone density; cholesterol; clinical examination; dual-energy X-ray absorptiometry; high density lipoprotein; hypercholesterolemia; inflammation; interleukin-6; low density lipoprotein; morbidity; muscle tissues; necrosis; neoplasms; nutrition; obesity; triacylglycerols
Abstract:
... Objectives: Normal weight obesity (NWO) is defined as a condition of normal body weight, but with high body fat percentage. Clinical and immunologic implications of NWO in persons living with HIV (PLHIV) remain unknown. The aim of this study was to examine NWO prevalence and its associations with metabolic and immunologic measurements in a cohort of PLHIV on antiretroviral treatment (ART).Methods: ...
DOI:
10.1016/j.nut.2021.111515

49. Novel Chinese Angelica Polysaccharide Biomimetic Nanomedicine to Curcumin Delivery for Hepatocellular Carcinoma Treatment and Immunomodulatory Effect

Author:
Chunjing Guo; Xiaoya Hou; Yanhui Liu; Yanchun Zhang; Haiyu Xu; Feng Zhao; Daquan Chen
Source:
Phytomedicine 2021 v.80 pp. 153356
ISSN:
0944-7113
Subject:
Angelica; CD8-positive T-lymphocytes; Oriental traditional medicine; biocompatibility; biomimetics; cancer therapy; curcumin; diagnostic techniques; dialysis; encapsulation; erythrocyte membrane; extrusion; hepatoma; hyaluronic acid; immunomodulators; interleukin-12; micelles; nanocarriers; nanomedicine
Abstract:
... Using natural polysaccharides from Traditional Chinese Medicine as nanodrug delivery systems have considerable potential for tumor diagnostics and therapeutics.On the basis of targeted therapy and combining the advantages of natural polysaccharides (angelica polysaccharide, APS) and natural Chinese medicine (curcumin, Cur) to design functionalized nanoparticles to improve the therapeutic through c ...
DOI:
10.1016/j.phymed.2020.153356

50. Prevention of CD8 T Cell Deletion during Chronic Viral Infection

Author:
David G. Brooks; Antoinette Tishon; Michael B. A. Oldstone; Dorian B. McGavern
Source:
Viruses 2021 v.13 no.7 pp. -
ISSN:
1999-4915
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; therapeutics; viral antigens; virus replication; viruses
Abstract:
... During chronic viral infections, CD8 T cells rapidly lose antiviral and immune-stimulatory functions in a sustained program termed exhaustion. In addition to this loss of function, CD8 T cells with the highest affinity for viral antigen can be physically deleted. Consequently, treatments designed to restore function to exhausted cells and control chronic viral replication are limited from the onse ...
DOI:
10.3390/v13071189

51. Pronounce expression of Tim-3 and CD39 but not PD1 defines CD8 T cells in critical Covid-19 patients

Author:
Mehdi Shahbazi; Zahra Moulana; Mahdi Sepidarkish; Mojgan Bagherzadeh; Maryam Rezanejad; Mohammad Mirzakhani; Mohammad Jafari; Mousa Mohammadnia-Afrouzi
Source:
Microbial pathogenesis 2021 v.153 pp. 104779
ISSN:
0882-4010
Subject:
CD8-positive T-lymphocytes; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; immune response; immunoglobulins; mucins; pathogenesis; phenotype; programmed cell death
Abstract:
... During viral infection, inhibitory receptors play a key role in regulating CD8 T-cell activity. The objective of this research was to investigate programmed cell death protein 1 (PD-1), T-cell immunoglobulin and mucin domain-containing protein-3 (TIM-3), and CD39 exhaustion markers in CD8 T cells of new coronavirus disease-2019 (COVID-19) patients. A total of 44 patients with COVID-19 (17 subjects ...
DOI:
10.1016/j.micpath.2021.104779

52. Pyroptosis-inducing active caspase-1 as a genetic adjuvant in anti-cancer DNA vaccination

Author:
Tsolere Arakelian; Koen Oosterhuis; Elena Tondini; Mandy Los; Jana Vree; Mariska van Geldorp; Marcel Camps; Bram Teunisse; Iris Zoutendijk; Ramon Arens; Gerben Zondag; Ferry Ossendorp; Jeroen van Bergen
Source:
Vaccine 2022 v.40 no.13 pp. 2087-2098
ISSN:
0264-410X
Subject:
CD8-positive T-lymphocytes; adjuvants; caspase-1; epitopes; interleukin-1beta; melanoma; necroptosis; ovalbumin; plasmids; pyroptosis; recombinant vaccines; transfection; vaccination
Abstract:
... Pyroptosis is a recently discovered form of inflammatory programmed necrosis characterized by caspase-1-mediated and gasdermin D-dependent cell death leading to the release of pro-inflammatory cytokines such as Interleukin-1 beta (IL-1β). Here, we evaluated whether pyroptosis could be exploited in DNA vaccination by incorporating a constitutively active variant of caspase-1 to the antigen-expressi ...
DOI:
10.1016/j.vaccine.2022.02.028

53. SARS-CoV-2 Accessory Protein ORF8 Decreases Antibody-Dependent Cellular Cytotoxicity

Author:
Guillaume Beaudoin-Bussières; Ariana Arduini; Catherine Bourassa; Halima Medjahed; Gabrielle Gendron-Lepage; Jonathan Richard; Qinghua Pan; Zhen Wang; Chen Liang; Andrés Finzi
Source:
Viruses 2022 v.14 no.6 pp. -
ISSN:
1999-4915
Subject:
CD8-positive T-lymphocytes; Severe acute respiratory syndrome coronavirus 2; cytotoxicity; immune evasion; monocytes
Abstract:
... Viruses use many different strategies to evade host immune responses. In the case of SARS-CoV-2, its Spike mutates rapidly to escape from neutralizing antibodies. In addition to this strategy, ORF8, a small accessory protein encoded by SARS-CoV-2, helps immune evasion by reducing the susceptibility of SARS-CoV-2-infected cells to the cytotoxic CD8+ T cell response. Interestingly, among all accesso ...
DOI:
10.3390/v14061237

54. Serum Leptin Levels, Nutritional Status, and the Risk of Healthcare-Associated Infections in Hospitalized Older Adults

Author:
Elena Paillaud; Johanne Poisson; Clemence Granier; Antonin Ginguay; Anne Plonquet; Catherine Conti; Amaury Broussier; Agathe Raynaud-Simon; Sylvie Bastuji-Garin
Source:
Nutrients 2022 v.14 no.1 pp. -
ISSN:
2072-6643
Subject:
C-reactive protein; CD8-positive T-lymphocytes; albumins; blood serum; confidence interval; leptin; malnutrition; nutrition assessment; nutritional status; odds ratio; regression analysis; risk
Abstract:
... We aimed to determine whether serum leptin levels are predictive of the occurrence of healthcare-associated infections (HAIs) in hospitalized older patients. In a prospective cohort, 232 patients had available data for leptin and were monitored for HAIs for 3 months. Admission data included comorbidities, invasive procedures, the Mini Nutritional Assessment (MNA), BMI, leptin, albumin and C-reacti ...
DOI:
10.3390/nu14010226

55. The genetic variability, phylogeny and functional significance of E6, E7 and LCR in human papillomavirus type 52 isolates in Sichuan, China

Author:
Zhilin Song; Yanru Cui; Qiufu Li; Junhang Deng; Xianping Ding; Jiaoyu He; Yiran Liu; Zhuang Ju; Liyuan Fang
Source:
Virology journal 2021 v.18 no.1 pp. 94
ISSN:
1743-422X
Subject:
B-lymphocytes; CD8-positive T-lymphocytes; Papillomaviridae; amino acids; carcinogenesis; databases; epitopes; genetic variation; humans; peptides; phylogeny; prediction; probability; selection pressure; therapeutics; transcription factors; uterine cervical neoplasms; vaccine development; virology; viruses; China
Abstract:
... BACKGROUND: Variations in human papillomavirus (HPV) E6 and E7 have been shown to be closely related to the persistence of the virus and the occurrence and development of cervical cancer. Long control region (LCR) of HPV has been shown multiple functions on regulating viral transcription. In recent years, there have been reports on E6/E7/LCR of HPV-16 and HPV-58, but there are few studies on HPV-5 ...
DOI:
10.1186/s12985-021-01565-5

56. Tissue-resident FOLR2+ macrophages associate with CD8+ T cell infiltration in human breast cancer

Author:
Rodrigo Nalio Ramos; Yoann Missolo-Koussou; Yohan Gerber-Ferder; Christian P. Bromley; Mattia Bugatti; Nicolas Gonzalo Núñez; Jimena Tosello Boari; Wilfrid Richer; Laurie Menger; Jordan Denizeau; Christine Sedlik; Pamela Caudana; Fiorella Kotsias; Leticia L. Niborski; Sophie Viel; Mylène Bohec; Sonia Lameiras; Sylvain Baulande; Laëtitia Lesage; André Nicolas; Didier Meseure; Anne Vincent-Salomon; Fabien Reyal; Charles-Antoine Dutertre; Florent Ginhoux; Lene Vimeux; Emmanuel Donnadieu; Bénédicte Buttard; Jérôme Galon; Santiago Zelenay; William Vermi; Pierre Guermonprez; Eliane Piaggio; Julie Helft
Source:
Cell 2022 v.185 no.7 pp. 1189-1207.e25
ISSN:
0092-8674
Subject:
CD8-positive T-lymphocytes; breast neoplasms; humans; immunity; macrophages; mammary glands; neoplasm progression; patients; therapeutics
Abstract:
... Macrophage infiltration is a hallmark of solid cancers, and overall macrophage infiltration correlates with lower patient survival and resistance to therapy. Tumor-associated macrophages, however, are phenotypically and functionally heterogeneous. Specific subsets of tumor-associated macrophage might be endowed with distinct roles on cancer progression and antitumor immunity. Here, we identify a d ...
DOI:
10.1016/j.cell.2022.02.021

57. Tripartite-Motif 21 (TRIM21) Deficiency Results in a Modest Loss of Herpes Simplex Virus (HSV)-1 Surveillance in the Trigeminal Ganglia Following Cornea Infection

Author:
Amanda Berube; Grzegorz B. Gmyrek; Derek J. Royer; Daniel J. J. Carr
Source:
Viruses 2022 v.14 no.3 pp. -
ISSN:
1999-4915
Subject:
CD8-positive T-lymphocytes; angiogenesis; cornea; gene expression; herpes simplex; immunoglobulins; interferons; monitoring; opacity; viruses; vision
Abstract:
... Tripartite-motif 21 (TRIM21) is thought to regulate the type I interferon (IFN) response to virus pathogens and serve as a cytosolic Fc receptor for immunoglobulin. Since herpes simplex virus (HSV)-1 is sensitive to type I IFN and neutralizing antibody, we investigated the role of TRIM21 in response to ocular HSV-1 infection in mice. In comparison to wild type (WT) mice, TRIM21 deficient (TRIM21 K ...
DOI:
10.3390/v14030589

58. VSIG4/CRIg directly regulates early CD8+ T cell activation through its counter-receptor in a narrow window

Author:
Amrita Widyagarini; Naoto Nishii; Yohei Kawano; Chenyang Zhang; Miyuki Azuma
Source:
Biochemical and biophysical research communications 2022 v.614 pp. 100-106
ISSN:
0006-291X
Subject:
CD8-positive T-lymphocytes; homeostasis; ligands; phenotype; research
Abstract:
... T-cell responses are fine-tuned by positive and negative co-signal molecules expressed on immune cells and adjacent tissues. VSIG4 is a newly identified member of the B7 family of ligands, which negatively regulates innate inflammatory and CD4⁺ T cell-mediated responses. However, little is known about the direct effects of VSIG4, which are exerted through an unidentified counter-receptor on CD8⁺ T ...
DOI:
10.1016/j.bbrc.2022.04.120

59. Vaccine nanodiscs plus polyICLC elicit robust CD8+ T cell responses in mice and non-human primates

Author:
Alireza Hassani Najafabadi; Zeynab Izadi Najaf Abadi; Marisa E. Aikins; Kathryn E. Foulds; Mitzi M. Donaldson; Wenmin Yuan; Emeka B. Okeke; Jutaek Nam; Yao Xu; Priyan Weerappuli; Taryn Hetrick; David Adams; Patrick A. Lester; Andres M. Salazar; Dan H. Barouch; Anna Schwendeman; Robert A. Seder; James J. Moon
Source:
Journal of controlled release 2021 v.337 pp. 168-178
ISSN:
0168-3659
Subject:
CD8-positive T-lymphocytes; Simian immunodeficiency virus; Toll-like receptor 9; agonists; antigens; comparative study; double-stranded RNA; lymph nodes; oligodeoxyribonucleotides; peptides; therapeutics
Abstract:
... Conventional cancer vaccines based on soluble vaccines and traditional adjuvants have produced suboptimal therapeutic efficacy in clinical trials. Thus, there is an urgent need for vaccine technologies that can generate potent T cell responses with strong anti-tumor efficacy. We have previously reported the development of synthetic high-density protein (sHDL) nanodiscs for efficient lymph node (LN ...
DOI:
10.1016/j.jconrel.2021.07.026

60. Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

Author:
Abishek Chandrashekar; Jingyou Yu; Katherine McMahan; Catherine Jacob-Dolan; Jinyan Liu; Xuan He; David Hope; Tochi Anioke; Julia Barrett; Benjamin Chung; Nicole P. Hachmann; Michelle Lifton; Jessica Miller; Olivia Powers; Michaela Sciacca; Daniel Sellers; Mazuba Siamatu; Nehalee Surve; Haley VanWyk; Huahua Wan; Cindy Wu; Laurent Pessaint; Daniel Valentin; Alex Van Ry; Jeanne Muench; Mona Boursiquot; Anthony Cook; Jason Velasco; Elyse Teow; Adrianus C.M. Boon; Mehul S. Suthar; Neharika Jain; Amanda J. Martinot; Mark G. Lewis; Hanne Andersen; Dan H. Barouch
Source:
Cell 2022 v.185 no.9 pp. 1549-1555.e11
ISSN:
0092-8674
Subject:
CD8-positive T-lymphocytes; Severe acute respiratory syndrome coronavirus 2; antibodies; nose; vaccines; viruses
Abstract:
... The rapid spread of the SARS-CoV-2 Omicron (B.1.1.529) variant, including in highly vaccinated populations, has raised important questions about the efficacy of current vaccines. In this study, we show that the mRNA-based BNT162b2 vaccine and the adenovirus-vector-based Ad26.COV2.S vaccine provide robust protection against high-dose challenge with the SARS-CoV-2 Omicron variant in cynomolgus macaq ...
DOI:
10.1016/j.cell.2022.03.024

61. A bioluminescence reporter mouse strain for in vivo imaging of CD8+ T cell localization and function

Author:
Yang Guo; Bingyin Wang; Yanjuan Chen; Min Liang; Haijie Wang; Chengji Wang; Hanwen Liang; Yu Zhou; Jun Xi; Lei Ci; Ruilin Sun; Jian Fei; Ruling Shen
Source:
Biochemical and biophysical research communications 2021 v.581 pp. 12-19
ISSN:
0006-291X
Subject:
CD8-positive T-lymphocytes; adaptive immunity; bioluminescence; genome; luciferase; mice; research
Abstract:
... CD8⁺ T cells play a critical role during adaptive immune response, which often change locations and expand or contract in numbers under different states. In the past, many attempts to develop CD8⁺T cells that express luciferase in vivo have involved the use of viral transduction, which has drawbacks of hardly tracked via detection of luciferase signal in untouched natural states. Here, we generate ...
DOI:
10.1016/j.bbrc.2021.10.022

62. A chimeric HLA-A2:β2M:Ig fusion protein for the study of virus-specific CD8+ T-cells

Author:
Ágata Lopes Ribeiro; Franklin Pereira Araújo; Julia Pereira Martins; Alice Aparecida Lourenço; Jing Huang; Felipe Valença Pereira; Luis Adan Flores Andrade; Adriana Alves Oliveira Paim; Flávio Guimarães da Fonseca; Edel Figueiredo Barbosa-Stancioli; Olindo Assis Martins-Filho; Vanessa Peruhype-Magalhães; Moriya Tsuji; Jordana G. Coelho-dos-Reis
Source:
Journal of immunological methods 2021 v.492 pp. 112997
ISSN:
0022-1759
Subject:
Adenoviridae; CD8-positive T-lymphocytes; animal models; antigens; humans; immunity; influenza; mice; peptides; vaccination
Abstract:
... The response mediated by CD8⁺ T-cells in the context of infection and vaccination has been thoroughly investigated and represents one of the most important branches that allow for the development of immunity against intracellular pathogens and, thus, the establishment of robust antiviral responses. However, there is a lack of methods to assess antigen-specific CD8⁺ T-cells.Search for the ideal ass ...
DOI:
10.1016/j.jim.2021.112997

63. A comparison of alternative mRNA splicing in the CD4 and CD8 T cell lineages

Author:
Xin Liu; Matthew V. Andrews; Jarrod P. Skinner; Timothy M. Johanson; Mark M.W. Chong
Source:
Molecular immunology 2021 v.133 pp. 53-62
ISSN:
0161-5890
Subject:
CD8-positive T-lymphocytes; alternative splicing; amino acid sequences; exons; immunology; introns; transcription (genetics); transcriptomics
Abstract:
... T cells can be subdivided into a number of different subsets that are defined by their distinct functions. While the specialization of different T cell subsets is partly achieved by the expression of specific genes, the overall transcriptional profiles of all T cells appear very similar. Alternative mRNA splicing is a mechanism that facilitates greater transcript/protein diversity from a limited n ...
DOI:
10.1016/j.molimm.2021.02.009

64. A tumor-specific neoepitope expressed in homologous/self or heterologous/viral antigens induced comparable effector CD8+ T-cell responses by DNA vaccination

Author:
Katja Stifter; Iryna Dekhtiarenko; Jana Krieger; Alain Charles Tissot; Thomas Seufferlein; Martin Wagner; Reinhold Schirmbeck
Source:
Vaccine 2020 v.38 no.21 pp. 3711-3719
ISSN:
0264-410X
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; DNA; amino acids; antibody formation; cancer vaccines; genetic vectors; immunoglobulin G; mice; neoplasms; phenotype; somatic mutation; vaccination; viral antigens
Abstract:
... Somatic mutations in tumors often generate neoproteins that contain MHC-I-binding neoepitopes. Little is known if and how efficient tumor-specific neoantigens activate CD8⁺ T cells. Here, we asked whether a de novo generated neoepitope, encoded either within an otherwise conserved and ubiquitously expressed self-antigen or in a chimeric HBV core antigen expression platform, providing heterologous ...
DOI:
10.1016/j.vaccine.2020.04.003

65. A unified atlas of CD8 T cell dysfunctional states in cancer and infection

Author:
Yuri Pritykin; Joris van der Veeken; Allison R. Pine; Yi Zhong; Merve Sahin; Linas Mazutis; Dana Pe’er; Alexander Y. Rudensky; Christina S. Leslie
Source:
Molecular cell 2021 v.81 no.11 pp. 2477-2493.e10
ISSN:
1097-2765
Subject:
CD8-positive T-lymphocytes; RNA; chromatin; dissection; immunity; neoplasms; sequence analysis; transcription (genetics)
Abstract:
... CD8 T cells play an essential role in defense against viral and bacterial infections and in tumor immunity. Deciphering T cell loss of functionality is complicated by the conspicuous heterogeneity of CD8 T cell states described across experimental and clinical settings. By carrying out a unified analysis of over 300 assay for transposase-accessible chromatin sequencing (ATAC-seq) and RNA sequencin ...
DOI:
10.1016/j.molcel.2021.03.045

66. Alteration of immunological parameters in infectious bronchitis vaccinated–specific pathogen-free broilers after the use of different infectious bursal disease vaccines

Author:
Caterina Lupini; Giulia Quaglia; Giulia Mescolini; Elisa Russo; Roberta Salaroli; Monica Forni; Sara Boldini; Elena Catelli
Source:
Poultry science 2020 v.99 no.9 pp. 4351-4359
ISSN:
0032-5791
Subject:
Avian orthoavulavirus 1; CD8-positive T-lymphocytes; Herpesvirales; antibodies; antigen-antibody complex; blood sampling; broiler chickens; bursa of Fabricius; enzyme-linked immunosorbent assay; flow cytometry; genetic engineering; immune response; inactivated vaccines; infectious bronchitis; infectious bursal disease; live vaccines; serology; specific pathogen-free animals; spleen; surface proteins; turkeys; vaccination; viruses
Abstract:
... The vaccines currently available to control infectious bursal disease (IBD) include live-attenuated and inactivated vaccines, immune-complex vaccines, and vaccines consisting of viral constructs of herpesvirus of turkeys genetically engineered to express VP2 surface protein. To evaluate the impact of vaccines on the chicken immune system, 2 animal trials were performed in specific pathogen-free br ...
DOI:
10.1016/j.psj.2020.05.054

67. Antibody-mediated delivery of T-cell epitopes to antigen-presenting cells induce strong CD4 and CD8 T-cell responses

Author:
Lene S. Høydahl; Terje Frigstad; Ingunn B. Rasmussen; Inger Øynebråten; Karoline W. Schjetne; Jan Terje Andersen; Terje E. Michaelsen; Elin Lunde; Bjarne Bogen; Inger Sandlie
Source:
Vaccine 2021 v.39 no.11 pp. 1583-1592
ISSN:
0264-410X
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; antibodies; antigen presentation; cytokines; epitopes; immunoglobulins; intravenous injection; secretion; vaccination; vaccines
Abstract:
... Targeted delivery of antigen to antigen-presenting cells (APCs) enhances antigen presentation and thus, is a potent strategy for making more efficacious vaccines. This can be achieved by use of antibodies with specificity for endocytic surface molecules expressed on the APC. We aimed to compare two different antibody-antigen fusion modes in their ability to induce T-cell responses; first, exchange ...
DOI:
10.1016/j.vaccine.2021.02.012

68. Assessment of the population coverage of an HIV-1 vaccine targeting sequences surrounding the viral protease cleavage sites in Gag, Pol, or all 12 protease cleavage sites

Author:
Mathew Daniel; Binhua Liang; Ma Luo
Source:
Vaccine 2021 v.39 no.19 pp. 2676-2683
ISSN:
0264-410X
Subject:
CD8-positive T-lymphocytes; databases; epitopes; proteinases; vaccines; world wide web
Abstract:
... Development of an effective HIV-1 vaccine has been a great challenge faced by the research community. Recently a novel strategy targeting the viral protease cleavage sites (PCSs) has been tested and shown promising results. This T cell-based vaccine strategy depends on individuals expressing certain HLA class I molecules and since each population has unique distributions of HLA class I alleles, po ...
DOI:
10.1016/j.vaccine.2021.03.068

69. Broad-Based Influenza-Specific CD8+ T Cell Response without the Typical Immunodominance Hierarchy and Its Potential Implication

Author:
Miaojuan Huang; Rong Xu; Cristina Triffon; Nicole Mifsud; Weisan Chen
Source:
Viruses 2021 v.13 no.6 pp. -
ISSN:
1999-4915
Subject:
CD8-positive T-lymphocytes; Influenza A virus; amino acids; epitopes; mice; models; prediction; Australia
Abstract:
... Syngeneic murine systems have pre-fixed MHC, making them an imperfect model for investigating the impact of MHC polymorphism on immunodominance in influenza A virus (IAV) infections. To date, there are few studies focusing on MHC allelic differences and its impact on immunodominance even though it is well documented that an individual’s HLA plays a significant role in determining immunodominance h ...
DOI:
10.3390/v13061080

70. CD4 T Cell-Mediated Immune Control of Cytomegalovirus Infection in Murine Salivary Glands

Author:
Nathan Zangger; Josua Oderbolz; Annette Oxenius
Source:
Pathogens 2021 v.10 no.12 pp. -
ISSN:
2076-0817
Subject:
B-lymphocytes; CD8-positive T-lymphocytes; Cytomegalovirus infections; Muromegalovirus; interferon-gamma; mechanism of action; mice; tumor necrosis factor-alpha
Abstract:
... CD4 T cells are well known for their supportive role in CD8 T cell and B cell responses during viral infection. However, during murine cytomegalovirus (MCMV) infection in the salivary glands (SGs), CD4 T cells exhibit direct antiviral effector functions to control the infection. In this mucosal organ, opposed to other infected tissues, MCMV establishes a sustained lytic replication that lasts for ...
DOI:
10.3390/pathogens10121531

71. CD4+ and CD8+ Circulating Memory T Cells Are Crucial in the Protection Induced by Vaccination with Salmonella Typhi Porins

Author:
Luis Ontiveros-Padilla; Alberto García-Lozano; Araceli Tepale-Segura; Tania Rivera-Hernández; Rodolfo Pastelin-Palacios; Armando Isibasi; Lourdes A. Arriaga-Pizano; Laura C. Bonifaz; Constantino López-Macías
Source:
Microorganisms 2021 v.9 no.4 pp. -
ISSN:
2076-2607
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; Salmonella Typhi; immune response; lymph nodes; memory; microbial load; phenotype; porins; vaccination
Abstract:
... Salmonella enterica serovar Typhi (S. Typhi) porins, OmpC and OmpF, are potent inducers of the immune response against S. Typhi in mice and humans. Vaccination with porins induces the protection against 500 LD₅₀ of S. Typhi, life-lasting bactericidal antibodies and effector T cell responses in mice; however, the nature of the memory T cell compartment and its contribution to protection remains unk ...
DOI:
10.3390/microorganisms9040770

72. CD8+ T cell/adipocyte inflammatory cross talk and ensuing M1 macrophage polarization are reduced by fish-oil-derived n-3 polyunsaturated fatty acids, in part by a TNF-α-dependent mechanism

Author:
Danyelle M. Liddle; Jennifer M. Monk; Amber L. Hutchinson; David W.L. Ma; Lindsay E. Robinson
Source:
Journal of nutritional biochemistry 2020 v.76 pp. 108243
ISSN:
0955-2863
Subject:
CD8-positive T-lymphocytes; Carthamus tinctorius; adipocytes; adipose tissue; anti-inflammatory activity; caspase-1; chemokine CCL2; chemokine CCL3; chemokine CCL7; chemotaxis; coculture; diet; endotoxins; fish; fish oils; gene expression; inflammasomes; inflammation; interferon-gamma; interleukin-1beta; interleukin-6; lipopolysaccharides; macrophages; messenger RNA; mice; models; neutralizing antibodies; omega-3 fatty acids; polyunsaturated fatty acids; protein secretion; safflower oil; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:
... Obese visceral adipose tissue (AT) inflammation is driven by adipokine-mediated cross talk between CD8⁺ T cells and adipocytes, a process mitigated by long-chain (LC) n-3 polyunsaturated fatty acids (PUFA) but underlying mechanisms and ensuing effects on macrophage polarization status are unknown. Using an in vitro co-culture model that recapitulates the degree of CD8⁺ T cell infiltration reported ...
DOI:
10.1016/j.jnutbio.2019.108243

73. Cellular Immune Response Induced by DNA Immunization of Mice with Drug Resistant Integrases of HIV-1 Clade A Offers Partial Protection against Growth and Metastatic Activity of Integrase-Expressing Adenocarcinoma Cells

Author:
Maria Isaguliants; Olga Krotova; Stefan Petkov; Juris Jansons; Ekaterina Bayurova; Dzeina Mezale; Ilze Fridrihsone; Athina Kilpelainen; Philip Podschwadt; Yulia Agapkina; Olga Smirnova; Linda Kostic; Mina Saleem; Oleg Latyshev; Olesja Eliseeva; Anastasia Malkova; Tatiana Gorodnicheva; Britta Wahren; Ilya Gordeychuk; Elizaveta Starodubova; Anastasia Latanova
Source:
Microorganisms 2021 v.9 no.6 pp. -
ISSN:
2076-2607
Subject:
CD8-positive T-lymphocytes; DNA; adenocarcinoma; antigens; antiretroviral agents; carcinogenicity; cell-mediated immunity; drug resistance; immunization; immunogenicity; immunotoxicity; integrases; metastasis; oxidative stress; proteolysis
Abstract:
... Therapeutic DNA-vaccination against drug-resistant HIV-1 may hinder emergence and spread of drug-resistant HIV-1, allowing for longer successful antiretroviral treatment (ART) up-to relief of ART. We designed DNA-vaccines against drug-resistant HIV-1 based on consensus clade A integrase (IN) resistant to raltegravir: IN_in_r1 (L74M/E92Q/V151I/N155H/G163R) or IN_in_r2 (E138K/G140S/Q148K) carrying D ...
DOI:
10.3390/microorganisms9061219

74. Characterization of Immune Cell Subsets of Tumor Infiltrating Lymphocytes in Brain Metastases

Author:
Priyakshi Kalita-de Croft; Haarika Chittoory; Tam H. Nguyen; Jodi M. Saunus; Woo Gyeong Kim; Amy E. McCart Reed; Malcolm Lim; Xavier M. De Luca; Kaltin Ferguson; Colleen Niland; Roberta Mazzieri; Riccardo Dolcetti; Peter T. Simpson; Sunil R. Lakhani
Source:
Biology 2021 v.10 no.5 pp. -
ISSN:
2079-7737
Subject:
CD8-positive T-lymphocytes; RNA; brain; fluorescent antibody technique; immunosuppression; metastasis; monitoring; neoplasms; neuroglia; phenotype
Abstract:
... The heterogeneity of tumor infiltrating lymphocytes (TILs) is not well characterized in brain metastasis. To address this, we performed a targeted analysis of immune-cell subsets in brain metastasis tissues to test immunosuppressive routes involved in brain metastasis. We performed multiplex immunofluorescence (mIF), using commercially available validated antibodies on formalin-fixed paraffin embe ...
DOI:
10.3390/biology10050425

75. Comparative analysis of key immune protection factors in H9N2 avian influenza viruses infected and immunized specific pathogen–free chicken

Author:
Manman Dai; Shibing Li; Keyi Shi; Hui Sun; Li Zhao; Deshui Yu; Jiayu Liao; Chenggang Xu; Ming Liao
Source:
Poultry science 2021 v.100 no.1 pp. 39-46
ISSN:
0032-5791
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; Influenza A virus; antibodies; chickens; cytokines; flocks; gene expression; genes; immune response; immunization; inactivated vaccines; lungs; China
Abstract:
... H9N2 avian influenza viruses (AIV) continue to circulate in vaccinated chicken flocks in China, which prompted us to investigate the differential immune protection factors induced by H9N2 AIV infection and immunization for analyzing the reason of protection deficiency of H9N2 AIV inactivated vaccine. In this study, we firstly explored virus-induced optimal immune responses in chicken after H9N2 AI ...
DOI:
10.1016/j.psj.2020.09.080

76. Control of memory CD8+ T cell longevity and effector functions by IL-15

Author:
Jeffrey C. Nolz; Martin J. Richer
Source:
Molecular immunology 2020 v.117 pp. 180-188
ISSN:
0161-5890
Subject:
CD8-positive T-lymphocytes; cell cycle; cell-mediated immunity; cytotoxicity; interleukin-15; longevity; natural killer cells; tissues
Abstract:
... IL-15 is a member of the common gamma chain family of cytokines and plays important roles in regulating several aspects of innate and adaptive immunity. Besides its established role in controlling homeostatic proliferation and survival of memory CD8+ T cells and natural killer cells, recent findings demonstrate that inflammatory IL-15 can also stimulate a variety of effector functions, such as enh ...
DOI:
10.1016/j.molimm.2019.11.011
PubMed:
31816491
PubMed Central:
PMC6928418

77. Coreceptor blockade targeting CD4 and CD8 allows acceptance of allogeneic human pluripotent stem cell grafts in humanized mice

Author:
Jiatao Li; Xisheng Li; Cai Liang; Lijun Ling; Zhiwei Chen; Chun Kwok Wong; Herman Waldmann; Kathy O. Lui
Source:
Biomaterials 2020 v.248 pp. 120013
ISSN:
0142-9612
Subject:
CD8-positive T-lymphocytes; allografting; biocompatible materials; embryonic stem cells; humans
Abstract:
... We have previously demonstrated that short-term coreceptor blockade with non-lytic monoclonal antibodies enables the long-term survival of fully allogeneic embryonic stem cell (ESC) transplants in mice. Here, we describe the use of Hu-PBL humanized mice to determine whether short-term coreceptor blockade with humanized anti-human CD4 and CD8 antibodies can achieve the same outcome towards human ES ...
DOI:
10.1016/j.biomaterials.2020.120013

78. Creatine in T Cell Antitumor Immunity and Cancer Immunotherapy

Author:
Bo Li; Lili Yang
Source:
Nutrients 2021 v.13 no.5 pp. -
ISSN:
2072-6643
Subject:
CD8-positive T-lymphocytes; bioenergy; creatine; dietary supplements; immunity; immunotherapy; mice; muscles; neoplasms
Abstract:
... Creatine is a broadly used dietary supplement that has been extensively studied for its benefit on the musculoskeletal system. Yet, there is limited knowledge regarding the metabolic regulation of creatine in cells beyond the muscle. New insights concerning various regulatory functions for creatine in other physiological systems are developing. Here, we highlight the latest advances in understandi ...
DOI:
10.3390/nu13051633

79. Development and immunogenic potentials of chitosan-saponin encapsulated DNA vaccine against avian infectious bronchitis coronavirus

Author:
Faruku Bande; Siti Suri Arshad; Mohd Hair Bejo; Abdul Rahman Omar; Hassan Moeini; Saeid Khadkodaei; Tan Sheau Wei; Yeap Swee Keong; Yusuf Abba; Ibrahim Abubakar Anka
Source:
Microbial pathogenesis 2020 v.149 pp. 104560
ISSN:
0882-4010
Subject:
CD8-positive T-lymphocytes; Infectious bronchitis virus; bioinformatics; chickens; chitosan; cloaca; cross immunity; egg production; encapsulation; flow cytometry; glycoproteins; histopathology; immunization; immunogenicity; infectious bronchitis; kidneys; mortality; pathogenesis; poultry industry; quantitative polymerase chain reaction; recombinant vaccines; saponins; serotypes; transcription (genetics); viruses; weight gain
Abstract:
... Infectious Bronchitis (IB) is an economically important avian disease that considerably threatens the global poultry industry. This is partly, as a result of its negative consequences on egg production, weight gain as well as mortality rate.The disease is caused by a constantly evolving avian infectious bronchitis virus whose isolates are classified into several serotypes and genotypes that demons ...
DOI:
10.1016/j.micpath.2020.104560

80. Development of thermosensitive resiquimod-loaded liposomes for enhanced cancer immunotherapy

Author:
Hua Zhang; Wei-Lun Tang; Azadeh Kheirolomoom; Brett Z. Fite; Bo Wu; Kenneth Lau; Mo Baikoghli; Marina Nura Raie; Spencer K. Tumbale; Josquin Foiret; Elizabeth S. Ingham; Lisa M. Mahakian; Sarah M. Tam; R. Holland Cheng; Alexander D. Borowsky; Katherine W. Ferrara
Source:
Journal of controlled release 2021 v.330 pp. 1080-1094
ISSN:
0168-3659
Subject:
CD8-positive T-lymphocytes; Toll-like receptor 7; agonists; animal models; breast neoplasms; ferrous sulfate; fever; hypertrophy; immunohistochemistry; immunostimulants; immunotherapy; intravenous injection; lipids; mammary neoplasms (animal); mice; neoplasm cells; remission; solubility; thermosensitivity; weight loss
Abstract:
... Resiquimod (R848) is a toll-like receptor 7 and 8 (TLR7/8) agonist with potent antitumor and immunostimulatory activity. However, systemic delivery of R848 is poorly tolerated because of its poor solubility in water and systemic immune activation. In order to address these limitations, we developed an intravenously-injectable formulation with R848 using thermosensitive liposomes (TSLs) as a delive ...
DOI:
10.1016/j.jconrel.2020.11.013

81. Efficient Control of Zika Virus Infection Induced by a Non-Replicating Adenovector Encoding Zika Virus NS1/NS2 Antigens Fused to the MHC Class II-Associated Invariant Chain

Author:
Loulieta Nazerai; Søren Buus; Anette Stryhn; Allan Randrup Thomsen; Jan Pravsgaard Christensen
Source:
Viruses 2021 v.13 no.11 pp. -
ISSN:
1999-4915
Subject:
Adenoviridae; CD8-positive T-lymphocytes; Zika virus; immunogenicity; vaccination; vaccines
Abstract:
... It is generally believed that a successful Zika virus (ZIKV) vaccine should induce neutralizing antibodies against the ZIKV envelope (E) protein to efficiently halt viral infection. However, E-specific neutralizing antibodies have been implicated in a phenomenon called antibody-dependent enhancement, which represents an ongoing concern in the flavivirus-vaccinology field. In this report, we invest ...
DOI:
10.3390/v13112215

82. Employing hypoxia characterization to predict tumour immune microenvironment, treatment sensitivity and prognosis in hepatocellular carcinoma

Author:
Fanhong Zeng; Yue Zhang; Xu Han; Min Zeng; Yi Gao; Jun Weng
Source:
Computational and Structural Biotechnology Journal 2021 v.19 pp. 2775-2789
ISSN:
2001-0370
Subject:
CD8-positive T-lymphocytes; biotechnology; carcinogenesis; cytokines; hepatoma; hypoxia; immunosuppression; immunotherapy; landscapes; metabolism; models; neoplasm progression; patients; phenotype; prognosis; resection; risk; transcriptome
Abstract:
... The hypoxic microenvironment was recognized as a major driving force of the malignant phenotype in hepatocellular carcinoma (HCC), which contributes to tumour immune microenvironment (TIM) remodeling and tumor progression. Dysregulated hypoxia-related genes (HRGs) result in treatment resistance and poor prognosis by reshaping tumor cellular activities and metabolism. Approaches to identify the rel ...
DOI:
10.1016/j.csbj.2021.03.033

83. Evaluation of protection induced by in vitro maturated BMDCs presenting CD8+ T cell stimulating peptides after a heterologous vaccination regimen in BALB/c model against Leishmania major

Author:
Atefeh Sadeghi Shermeh; Farnaz Zahedifard; Sima Habibzadeh; Tahereh Taheri; Sima Rafati; Negar Seyed
Source:
Experimental parasitology 2021 v.223 pp. 108082
ISSN:
0014-4894
Subject:
CD8-positive T-lymphocytes; DNA; Leishmania major; cutaneous leishmaniasis; dendritic cells; epitopes; mice; models; oligodeoxyribonucleotides; parasites; parasitology; peptides; proteome; recombinant vaccines; vaccination; vector-borne diseases
Abstract:
... Leishmaniasis is a complex vector-borne disease mediated by Leishmania parasite and a strong and long-lasting CD4⁺ Th1 and CD8⁺-T cell immunity is required to control the infection. Thus far multivalent subunit vaccines have met this requirement more promisingly. However several full protein sequences cannot be easily arranged in one construct. Instead, new emerging immune-informatics based epitop ...
DOI:
10.1016/j.exppara.2021.108082

84. Evaluation of the Immunomodulatory and Anti-Inflammatory Activities of Honey Bee Larva Powder

Author:
Kejuan Li; Shuang Sun; Masakatsu Kageyama; Long Xiao; Guoqing Xing; Ran Gao; Fengming You; Xi Fu; Zhenya Zhang
Source:
Journal of medicinal food 2020 v.23 no.7 pp. 772-782
ISSN:
1557-7600
Subject:
CD8-positive T-lymphocytes; anti-inflammatory activity; apoptosis; cell proliferation; color; doxorubicin; freeze drying; health promotion; immunomodulators; in vitro studies; insect larvae; interleukin-6; lipopolysaccharides; macrophages; mice; neoplasms; nitric oxide; oral administration; spleen; toxicity
Abstract:
... Honey bee larva powder (HLP) has traditionally been used as a daily supplement and tonic for health promotion with an uncertain scientific basis. In this study, B16-F10 tumor-bearing mice were established to evaluate the immunomodulatory activity of HLP. The proliferation and apoptosis assays were performed to evaluate the anti-inflammatory activity of honey bee larva extract (HLE) in RAW 264.7 ma ...
DOI:
10.1089/jmf.2019.4554

85. Experimental study of Forsythoside A on prevention and treatment of avian infectious bronchitis

Author:
Xuebing Wang; Xiao Li; Xuefang Wang; Ling Chen; Erjuan Ning; Yi Fan; Hao Wang; Tong Chen; Wei Wang
Source:
Research in veterinary science 2021 v.135 pp. 523-531
ISSN:
0034-5288
Subject:
CD8-positive T-lymphocytes; Forsythia suspensa; Infectious bronchitis virus; Oriental traditional medicine; active ingredients; histopathology; immunomodulation; infectious bronchitis; interleukin-2; research; therapeutics; veterinary medicine
Abstract:
... Forsythoside A is the main active ingredient in the Chinese medicine Forsythia suspensa, which has antiviral, anti-inflammatory, antioxidation, and immunoregulatory effects. It is reported that Forsythoside A can significantly inhibit the replication of the avian infectious bronchitis virus(IBV) in cells, but there is no report in chickens. The present study aimed to investigate the effect of Fors ...
DOI:
10.1016/j.rvsc.2020.11.009

86. First detection of Theileria parva in cattle from Cameroon in the absence of the main tick vector Rhipicephalus appendiculatus

Author:
Barberine A. Silatsa; Gustave Simo; Naftaly Githaka; Rolin Kamga; Farikou Oumarou; Christian Keambou Tiambo; Eunice Machuka; Jean‐Baka Domelevo; David Odongo; Richard Bishop; Jules‐Roger Kuiate; Flobert Njiokou; Appolinaire Djikeng; Roger Pelle
Source:
Transboundary and emerging diseases 2020 v.67 Suppl S1 pp. 68-78
ISSN:
1865-1674
Subject:
CD8-positive T-lymphocytes; DNA; Rhipicephalus appendiculatus; Theileria mutans; Theileria parva; agroecological zones; antibodies; antigens; blood sampling; cattle; cross-sectional studies; disease transmission; disease vectors; emerging diseases; enzyme-linked immunosorbent assay; genes; grazing; livestock diseases; molecular cloning; monitoring; oligonucleotide probes; pathogens; polymerase chain reaction; risk factors; screening; sequence analysis; signs and symptoms (animals and humans); surveys; theileriosis; ticks; trade; Cameroon
Abstract:
... A major risk factor for the spread of livestock diseases and their vectors is the uncontrolled transboundary movement of live animals for trade and grazing. Such movements constrain effective control of tick‐transmitted pathogens, including Theileria parva. Only limited studies have been undertaken to identify ticks and tick‐borne diseases (TTBDs) affecting cattle in central African countries, inc ...
DOI:
10.1111/tbed.13425
PubMed:
32174039
PubMed Central:
PMC7216920

87. Flt3-L enhances trans-epithelial migration and antigen presentation of dendritic cells adoptively transferred to genital mucosa

Author:
Jaehyung Park; Hannah Frizzell; Hangyu Zhang; Shijie Cao; Sean M. Hughes; Florian Hladik; David M. Koelle; Kim A. Woodrow
Source:
Journal of controlled release 2021 v.329 pp. 782-793
ISSN:
0168-3659
Subject:
CD8-positive T-lymphocytes; antigen presentation; chitosan; dendritic cells; enzymes; female genitalia; female reproductive system; intravenous injection; ligands; lymph nodes; mice; mucosa; tyrosine; vaccination
Abstract:
... Dendritic cells (DCs) play a critical role in shaping adaptive immunity. Systemic transfer of DCs by intravenous injection has been widely investigated to inform the development of immunogenic DCs for use as cellular therapies. Adoptive transfer of DCs to mucosal sites has been limited but serves as a valuable tool to understand the role of the microenvironment on mucosal DC activation, maturation ...
DOI:
10.1016/j.jconrel.2020.10.012

88. Germinated black soybean fermented with Lactobacillus pentosus SC65 alleviates DNFB-induced delayed-type hypersensitivity in C57BL/6N mice

Author:
Hye-Ji Lee; Ha-Eun Cho; Hye-Jin Park
Source:
Journal of ethnopharmacology 2021 v.265 pp. 113236
ISSN:
0378-8741
Subject:
2,2-diphenyl-1-picrylhydrazyl; CD8-positive T-lymphocytes; Lactobacillus pentosus; Oriental traditional medicine; Pediococcus pentosaceus; Rhynchosia; anti-inflammatory activity; antioxidant activity; chemokine CCL5; daidzein; delayed hypersensitivity; dexamethasone; diabetes; ears; ethanol; foods; genistein; glycitein; hyperplasia; hypertension; immunity; immunohistochemistry; inflammation; interleukin-10; interleukin-1beta; interleukin-6; lactic acid bacteria; lipopolysaccharides; macrophages; messenger RNA; mice; natural killer cells; osteoporosis; permeability; polyphenols; reverse transcriptase polymerase chain reaction; soybeans; tumor necrosis factors; East Asia
Abstract:
... Rhynchosia nulubilis (black soybean) has many applications in oriental medicine. It is traditionally used to treat disease related with high blood pressure, diabetes, inflammation, and osteoporosis. Furthermore, fermented soybean foods have traditionally been used for immunity enhancement in East Asia. However, the anti-inflammatory effects of germinated R. nulubilis (GR) against delayed-type hype ...
DOI:
10.1016/j.jep.2020.113236

89. Goose Nephritic Astrovirus Infection of Goslings Induces Lymphocyte Apoptosis, Reticular Fiber Destruction, and CD8 T-Cell Depletion in Spleen Tissue

Author:
Rui Ding; Han Huang; Hongyu Wang; Zewen Yi; Siyu Qiu; Yingjun Lv; Endong Bao
Source:
Viruses 2021 v.13 no.6 pp. -
ISSN:
1999-4915
Subject:
CD8-positive T-lymphocytes; apoptosis; chromatin; gout; industry; macrophages; mitochondria; mortality; necrosis; plasma membrane; protein synthesis; pulp; spleen; viral load
Abstract:
... The emergence of a novel goose nephritic astrovirus (GNAstV) has caused economic losses to the Chinese goose industry. High viral load is found in the spleen of goslings infected with GNAstV, but pathological injuries to the spleen due to GNAstV are largely unknown. In this study, 50 two-day-old goslings were infected orally with GNAstV, and 50 goslings were treated with PBS as control. Spleens we ...
DOI:
10.3390/v13061108

90. Humoral and Cell-Mediated Immune Response Validation in Calves after a Live Attenuated Vaccine of Babesia bigemina

Author:
Umber Rauf; Muhammad Suleman; Asadullah Abid; Hamna Jamil; Harish Menghwar; Aneela Zameer Durrani; Muhammad Imran Rashid; Haroon Akbar
Source:
Pathogens 2020 v.9 no.11 pp. -
ISSN:
2076-0817
Subject:
Babesia bigemina; CD8-positive T-lymphocytes; antibodies; babesiosis; calves; cattle industry; cell-mediated immunity; erythrocytes; farmers; financial economics; humoral immunity; infection; intravenous injection; live vaccines; pathogens; vaccination; virulence
Abstract:
... The current vaccines to control bovine Babesia bigemina (B. bigemina) infection are not fully protective and vaccination failures incur heavy losses to the cattle industry around the world. Using modified micro-aerophilous stationary phase, we developed a culture-derived attenuated live vaccine against B. bigemina and tested a single subcutaneous inoculation of 2 × 10⁸ infected erythrocytes in cal ...
DOI:
10.3390/pathogens9110936

91. Identification of a superagonist variant of the immunodominant Yellow fever virus epitope NS4b 214-222 by combinatorial peptide library screening

Author:
Amandine Bovay; Vincent Zoete; Pierre J. Rizkallah; Konrad Beck; Philippe Delbreil; Daniel E. Speiser; David K. Cole; Silvia A. Fuertes Marraco
Source:
Molecular immunology 2020 v.125 pp. 43-50
ISSN:
0161-5890
Subject:
CD8-positive T-lymphocytes; Yellow fever virus; computer simulation; entropy; epitopes; humans; isoleucine; ligands; major histocompatibility complex; methionine; mutation; peptide libraries; viral nonstructural proteins
Abstract:
... The CD8 T cell response to the HLA-A2-restricted epitope LLWNGPMAV (LLW) of the non-structural protein 4b of Yellow Fever Virus (YFV) is remarkably immunodominant, highly prevalent and powerful in YFV-vaccinated humans. Here we used a combinatorial peptide library screening in the context of an A2/LLW-specific CD8 T cell clone to identify a superagonist that features a methionine to isoleucine sub ...
DOI:
10.1016/j.molimm.2020.06.025

92. Immunological exhaustion and functional profile of CD8+ T lymphocytes as cellular biomarkers of therapeutic efficacy in chronic Chagas disease patients

Author:
Elena Pérez-Antón; Adriana Egui; Mª Carmen Thomas; Marina Simón; Manuel Segovia; Manuel Carlos López
Source:
Acta tropica 2020 v.202 pp. 105242
ISSN:
0001-706X
Subject:
CD8-positive T-lymphocytes; Chagas disease; Trypanosoma cruzi; benznidazole; biomarkers; cytokines; cytotoxicity; interferon-gamma; monitoring; patients; phenotype; receptors; therapeutics
Abstract:
... The lack of useful tools for detection the impact of treatment during the follow-up of chronic Chagas disease treated patients difficult the adequate care to the affected population. The objective of this study was to evaluate the functional response of CD8⁺ T lymphocyte population, critical for the control of Trypanosoma cruzi infection, as a possible cellular biomarker of treated Chagas disease ...
DOI:
10.1016/j.actatropica.2019.105242

93. Immunophenotypic characterization and clinical outcome in cats with lymphocytosis

Author:
Emily D. Rout; Julia D. Labadie; Kaitlin M. Curran; Janna A. Yoshimoto; Anne C. Avery; Paul R. Avery
Source:
Journal of veterinary internal medicine 2020 v.34 no.1 pp. 105-116
ISSN:
0891-6640
Subject:
B-lymphocytes; CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; antigens; cats; disease course; females; flow cytometry; gene rearrangement; intestines; lymphatic diseases; medical records; phenotype; retrospective studies
Abstract:
... BACKGROUND: Lymphocytosis is relatively common in cats, but few studies describe lymphocyte populations or the clinical course associated with different immunophenotypic expansions. HYPOTHESIS/OBJECTIVES: We hypothesized that cats frequently develop non‐neoplastic lymphocytosis and that different neoplastic immunophenotypes have variable prognoses. We aimed to characterize the lymphocyte expansion ...
DOI:
10.1111/jvim.15650
PubMed:
31693230
PubMed Central:
PMC6979106

94. Immunostimulatory and antioxidant activities of a lignin isolated from Conocarpus erectus leaves

Author:
Dayane Kelly Dias do Nascimento Santos; Bárbara Rafaela da Silva Barros; Lethícia Maria de Souza Aguiar; Iranildo José da Cruz Filho; Virgínia Maria Barros de Lorena; Cristiane Moutinho Lagos de Melo; Thiago Henrique Napoleão
Source:
International journal of biological macromolecules 2020 v.150 pp. 169-177
ISSN:
0141-8130
Subject:
2,2-diphenyl-1-picrylhydrazyl; CD8-positive T-lymphocytes; Conocarpus erectus; Fourier transform infrared spectroscopy; antioxidant activity; antioxidants; calcium; carbon; cytokines; gel chromatography; humans; immune response; immunostimulants; inhibitory concentration 50; leaves; lignin; lipid peroxidation; mitochondria; molecular weight; monocytes; nitric oxide; nuclear magnetic resonance spectroscopy; spectral analysis; stable isotopes
Abstract:
... In this work, we investigated the antioxidant and immunomodulatory activities of a lignin isolated from Conocarpus erectus leaves. The lignin was characterized by FTIR, ¹H NMR, ¹³C NMR and gel permeation chromatography analysis as well as ultraviolet/visible absorption spectra. The lignin was evaluated for total antioxidant activity (TAA), DPPH and ABTS⁺ scavenging abilities, and by a lipid peroxi ...
DOI:
10.1016/j.ijbiomac.2020.02.052

95. Implementation of in silico methods to predict common epitopes for vaccine development against Chikungunya and Mayaro viruses

Author:
Hammadul Hoque; Rahatul Islam; Srijon Ghosh; Md. Mashiur Rahaman; Nurnabi Azad Jewel; Md. Abunasar Miah
Source:
Heliyon 2021 v.7 no.3 pp. e06396
ISSN:
2405-8440
Subject:
B-lymphocytes; CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; Chikungunya virus; Mayaro virus; Neotropics; computer simulation; epitopes; immunoinformatics; peptides; polyproteins; vaccine development; vaccines
Abstract:
... Being a Positive sense RNA virus the recent reemergence of Chikungunya and Mayaro virus has taken the concern of the leading scientific communities of the world. Though the outbreak of Mayaro virus is limited to Neotropical region only, Chikungunya is already identified in over 60 countries around the world. Besides, the lack of a strong protective treatment, misdiagnosis issue and co-circulation ...
DOI:
10.1016/j.heliyon.2021.e06396

96. In vitro platform establishes antigen-specific CD8+ T cell cytotoxicity to encapsulated cells via indirect antigen recognition

Author:
Ying Li; Anthony W. Frei; Ethan Y. Yang; Irayme Labrada-Miravet; Chuqiao Sun; Yanan Rong; Magdalena M. Samojlik; Allison L. Bayer; Cherie L. Stabler
Source:
Biomaterials 2020 v.256 pp. 120182
ISSN:
0142-9612
Subject:
CD8-positive T-lymphocytes; adaptive immunity; alginates; animals; antigens; biocompatible materials; cytotoxicity; hydrogels; microencapsulation; ovalbumin; therapeutics
Abstract:
... The curative potential of non-autologous cellular therapy is hindered by the requirement of anti-rejection therapy. Cellular encapsulation within nondegradable biomaterials has the potential to inhibit immune rejection, but the efficacy of this approach in robust preclinical and clinical models remains poor. While the responses of innate immune cells to the encapsulating material have been charact ...
DOI:
10.1016/j.biomaterials.2020.120182

97. Injectable shear-thinning polylysine hydrogels for localized immunotherapy of gastric cancer through repolarization of tumor-associated macrophages

Author:
Yan Yang; Yang Yang; Meili Chen; Jianquan Chen; Jinyan Wang; Yajun Ma; Hanqing Qian
Source:
Biomaterials science 2021 v.9 no.19 pp. 6597-6608
ISSN:
2047-4849
Subject:
CD8-positive T-lymphocytes; biocompatible materials; hydrogels; immunosuppression; immunotherapy; interleukin-6; macrophages; models; phenotype; polyethylene glycol; schiff bases; stomach neoplasms; xenotransplantation
Abstract:
... Immunotherapy has emerged as one of the most promising treatments for cancer in recent years. However, it works only for a small proportion of patients, which can in part be attributed to the immunosuppressive tumor microenvironment (TME). Tumor associated macrophages (TAMs) are the critical components of tumors and play an important role in the development of the immunosuppressive TME. The transi ...
DOI:
10.1039/d1bm01053k

98. Interferon-α2b Treatment for COVID-19 Is Associated with Improvements in Lung Abnormalities

Author:
Qiong Zhou; Michael R. MacArthur; Xinliang He; Xiaoshan Wei; Payam Zarin; Bola S. Hanna; Zi-Hao Wang; Xuan Xiang; Eleanor N. Fish
Source:
Viruses 2020 v.13 no.1 pp. -
ISSN:
1999-4915
Subject:
C-reactive protein; CD8-positive T-lymphocytes; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; albumins; biomarkers; death; interleukin-6; lungs; respiratory tract diseases; therapeutics
Abstract:
... Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection causes coronavirus disease 2019 (COVID-19), a lung disease that may progress to systemic organ involvement and in some cases, death. The identification of the earliest predictors of progressive lung disease would allow for therapeutic intervention in those cases. In an earlier clinical study, individuals with moderate COVID-19 ...
DOI:
10.3390/v13010044

99. Intraepithelial T cells diverge by intestinal location as pigs age

Author:
Wiarda Jayne E.; Julian M. Trachsel; Zahra F. Bond; Kristen A. Bryne; Nicholas K. Gabler; Crystal L. Loving
Source:
Frontiers in immunology 2020 v.11 no. pp. 1139
ISSN:
1664-3224
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes; animal age; epithelial cells; ileum; intestinal mucosa; large intestine; lymphocyte proliferation; swine
Abstract:
... T cells resident within the intestinal epithelium play a central role in barrier integrity and provide a first line of immune defense. Intraepithelial T cells (IETs) are among the earliest immune cells to populate and protect intestinal tissues, thereby giving them an important role in shaping gut health early in life. In pigs, IETs are poorly defined, and their maturation in young pigs has not be ...
Handle:
10113/7019860
DOI:
10.3389/fimmu.2020.01139

100. Intrauterine Fetal Demise After Uncomplicated COVID-19: What Can We Learn from the Case?

Author:
Pavel Babal; Lucia Krivosikova; Lucia Sarvaicova; Ivan Deckov; Tomas Szemes; Tatiana Sedlackova; Michal Palkovic; Anna Kalinakova; Pavol Janega
Source:
Viruses 2021 v.13 no.12 pp. -
ISSN:
1999-4915
Subject:
B-lymphocytes; CD8-positive T-lymphocytes; COVID-19 infection; RNA; Severe acute respiratory syndrome coronavirus 2; case studies; death; disease course; fetus; hybridization; immunohistochemistry; ischemia; lungs; maternal immunity; necropsy; neutrophils; nucleocapsid; patients; pregnancy; transplacental transmission; trophoblast; viruses
Abstract:
... Background: SARS-CoV-2 infection in pregnant women can lead to placental damage and transplacental infection transfer, and intrauterine fetal demise is an unpredictable event. Case study: A 32-year-old patient in her 38th week of pregnancy reported loss of fetal movements. She overcame mild COVID-19 with positive PCR test 22 days before. A histology of the placenta showed deposition of intervillou ...
DOI:
10.3390/v13122545