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DNA; Southern blotting; DNA probes; genetic variation; plant diseases and disorders; Prunus; Phytoplasma; Germany; California; Southern European region
Aquilegia; population genetics; genetic variation; DNA; genetic markers; hybrids; interspecific hybridization; altitude; introgression; plant morphology; natural selection; random amplified polymorphic DNA technique; population; California
Abstract:
... Habitat preference and pollination syndrome have been suggested as major factors in reproductive isolation among plant species. The columbine genus Aquilegia contains species that have been used as classic examples of reproductive isolation due to ecological and floral factors. In this analysis Aquilegia formosa, Aquilegia pubescens, and natural hybrid populations between these two species were as ...
... I used DNA fingerprinting to provide the first analysis of the genetic composition of western pond turtle (Clemmys marmorata(populations in Washington, Oregon, and California. Populations of the western pond turtle in Washington and northern Oregon are rapidly approaching extinction. Genetic similarity within the largest northern populations, which are located inland, is high. An analysis of popul ...
... The diversity of mechanisms causing insensitivity to phenylamide fungicides in Phytophthora infestans was addressed by comparative genetic analyses of isolates from North America, Europe, and Mexico. Both semi-dominant major loci (MEX loci) and genes of minor effect were previously shown to determine insensitivity based on studies of isolates from Europe and Mexico. In this investigation, genetic ...
... Visible and nonvisible quiescent infections of immature and mature fruit are an integral component of the disease cycle of brown rot of sweet cherry in California. Detection of these infections is critical for developing efficient and efficacious fungicide management programs. The previously published DNA amplification primers mfs3 and NS5 for the identification of Monilinia fructicola were very s ...
soil fungi; agricultural soils; suppressive soils; ribosomal RNA; Zygomycota; ribosomal DNA; Ascomycota; polymerase chain reaction; Persea americana; DNA primers; genes; nucleotide sequences; species diversity; Basidiomycota; Mastigomycotina; leaves; California
Abstract:
... Two PCR primer pairs were designed to amplify rRNA genes (rDNA) from all four major phyla of fungi: Ascomycota, Basidiomycota, Chytridomycota, and Zygomycota. PCRs performed with these primers showed that both pairs amplify DNA from organisms representing the major taxonomic groups of fungi but not from nonfungal sources. To test the ability of the primers to amplify fungal rDNA from environment s ...
swarming; microsatellite repeats; loci; Vespidae; DNA primers; DNA; polymerase chain reaction; phylogeny; heterozygosity; Queensland; Venezuela; Kenya; California
Abstract:
... We developed 52 microsatellite loci for the wasp, Polybioides tabidus, for the purpose of studying the evolution and inclusive fitness consequences of swarm-founding. The large number of loci is important for three reasons that may apply to many other systems. Heterozygosity was low in our target species, yet we found enough polymorphic loci for accurate kinship studies in this species. Many monom ...
DNA; active sites; agarose; cats; databases; dogs; exons; gel electrophoresis; humans; medical education; mutation; neoplasms; polymerase chain reaction; proto-oncogenes; therapeutics; California
Abstract:
... Objective-To evaluate splenic mast cell tumors (MCT) of cats for activating mutations in the protooncogene c-kit. Sample Population-10 formalin-fixed, paraffinembedded splenic MCT from cats in the pathology database of the Veterinary Medical Teaching Hospital at the University of California, Davis. Procedure-Genomic DNA was isolated from tumor specimens, and the polymerase chain reaction (PCR) pro ...
... Highly self-fruitful almonds have been developed which combine self-compatibility with autogamy (self-pollination). Pollen-pistil self-compatibility is known to be determined by a single genetic locus (S-locus). Specific SSC-alleles conferring self-compatibility, along with the major self-incompatibility alleles controlling cross-incompatibility in California cultivars, have been identified throug ...
DNA; databases; dogs; excision; exons; medical education; metastasis; mutation; neoplasms; polymerase chain reaction; prognosis; regression analysis; California
Abstract:
... Objective-To determine the prevalence of activating internal tandem duplications (ITDs) in exons 11 and 12 of c-kit in mast cell tumors (MCTs) of dogs and to correlate these mutations with prognosis. Sample Population-157 formalin-fixed, paraffinembedded MCTs from dogs in the pathology database of the Veterinary Medical Teaching Hospital at the University of California, Davis. Procedure-Genomic DN ...
Phoenix dactylifera; DNA; genetic variation; nucleotide sequences; varieties; mutants; polymerase chain reaction; genomics; California; United Arab Emirates
Abstract:
... Representational difference analysis was applied to subtract the genomes of the two date palm varieties, Barhee and Medjool, for identification and characterization of unique genome differences suitable for discriminating between individual plants and the two varieties. Three different DNA difference products were isolated from Barhee representing families of dispersed, repeated variable sequences ...
DNA; Verticillium dahliae; Verticillium wilt; coasts; crops; disease incidence; disease outbreaks; greenhouses; hot peppers; inoculum density; intensive cropping; mycelium; paprika; pathogenicity; pepper; plant pathology; random amplified polymorphic DNA technique; sclerotia; seedlings; soil; sweet peppers; temperature; tomatoes; California
Abstract:
... Epidemics of Verticillium wilt in pepper fields of the central coast of California and isolates of Verticillium dahliae associated with these epidemics were characterized. The mean incidence of wilted plants per field ranged from 6.3 to 97.8% in fields with Anaheim, jalapeno, paprika, or bell peppers. In general, incidence of wilt in jalapeno and bell pepper crops was lower than in crops of other ...
DNA; Helminthosporium solani; alanine; benomyl; cross resistance; fungicide resistance; genes; glutamic acid; plant pathology; point mutation; polymerase chain reaction; population; potatoes; restriction fragment length polymorphism; thiophanate-methyl; tubulin; California
Abstract:
... Helminthosporium solani populations on potato from the Tulelake area in California frequently have been exposed to thiophanate-methyl but not benomyl. Assessment of three H. solani populations found that 182 of 238 isolates were resistant to both thiophanate-methyl and benomyl and 56 isolates were sensitive to both fungicides. None of the isolates exhibited a differential reaction to the two fungi ...
... In the past decade, a new Phytophthora species inducing shoot canker on Rhododendron and dieback of Viburnum has been observed in Europe, mainly in Germany and the Netherlands, and California. This new pathogen has been named Phytophthora ramorum (3). In May 2002, a diseased Viburnum plant (Viburnum bodnantense) from the Plant Protection Service (Ministry of Agriculture, Belgium) was submitted to ...
Cyperus esculentus; Cyperus rotundus; DNA; ecotypes; genetic techniques and protocols; genetic variation; hybrids; interspecific variation; isozymes; morphometry; reproduction; California
Abstract:
... The identities of two novel perennial nutsedge biotypes collected near Bakersfield, CA, were assessed using isozyme and random-amplified polymorphic deoxyribonucleic acid markers in conjunction with morphological analysis. The two biotypes, designated as CK (Cyperus rotundus cv. ‘‘Kempeni’’) and CR (Cyperus esculentus cv. ‘‘Robusta’’), morphologically resemble purple nutsedge and yellow nutsedge, ...
... The resistance levels of different human head louse populations from the USA to 1% permethrin were evaluated using permethrin-impregnated, filter paper disk-contact bioassay. Populations from southern California, south Florida and south central Texas showed 1.5-, 3.1-, and 1.5- to 5.1-fold resistance compared to insecticide-susceptible head louse populations from Panama or Ecuador. Permethrin-resi ...
... Outside of Asia exotic tilapiine fishes (Trewavas 1983) were not imported directly as native genetic resources from Africa but arrived as transits from third or fourth party sources. Founder populations of exotic tilapia species may be morphologically and meristically distinct in Africa but are still reproductively compatible due to their relatively recent divergence. As a result, feral tilapias h ...
... Failure to control Alternaria late blight in a few California pistachio orchards was observed after only 3–4 years of consecutive applications of azoxystrobin-based fungicide programs. A total of 72 isolates of Alternaria alternata, Alternaria tenuissima, and Alternaria arborescens, the causal organisms of Alternaria late blight, were collected from pistachio orchards with (58 isolates) and withou ...
DNA; DNA libraries; Limnanthes; breeding; chromosome mapping; clones; genetic markers; genomics; haplotypes; loci; microsatellite repeats; oilseeds; British Columbia; California; Oregon
Abstract:
... The Limnanthaceae (Order Brassicales) is a family of 18 taxa of Limnanthes (meadowfoam) native to California, Oregon, and British Columbia. Cultivated meadowfoam (L. alba Benth.), a recently domesticated plant, has been the focus of research and development as an industrial oilseed for three decades. The goal of the present research was to develop several hundred simple sequence repeat (SSR) marke ...
... Giant reed is one of the most widespread invasive species in riparian habitats in California and other coastal states of the United States. This species is thought to spread primarily asexually by flood dispersal of stem and rhizome pieces; viable seeds have not been found in the United States. Research was conducted to quantify genetic variation in giant reed along the Santa Ana River in Californ ...
dairy cattle; pulsed-field gel electrophoresis; strains; cattle diseases; bacterial contamination; Salmonella enterica subsp. enterica; DNA; multiple drug resistance; bulk milk; feces; water pollution; human diseases; animal pathogenic bacteria; calves; antibiotics; food contamination; milk tanks; longitudinal studies; salmonellosis; food pathogens; California
Abstract:
... Recently, multi-drug-resistant (MDR) Salmonella enterica subspecies enterica serovar Newport reemerged as a public and animal health problem. The antibiotic resistance of 198 isolates and the pulsed-field gel electrophoresis patterns (PFGE) of 139 isolates were determined. Serovar Newport isolates collected between 1988 and 2001 were included in the study. One hundred seventy-eight isolates were c ...
Cynodon dactylon; DNA; DNA fingerprinting; cultivars; forage; genetic background; genetic relationships; genetic variation; germplasm; lawns and turf; new variety; tropics; Arizona; California; Colorado River; Yukon Territory
Abstract:
... Bermudagrasses (Cynodon spp.) are important for turf and forage in temperate and tropical climates, with cultivars historically propagated clonally. Over the past two decades the number of seed-propagated commercial cultivars has dramatically increased, but information is lacking on the extent of the genetic diversity among these new cultivars. Accordingly, this research was undertaken to assess t ...
... Understanding patterns of connectivity among marine fish populations with demersal adults and pelagic larvae is critical for effective conservation of west coast rockfishes. The brown rockfish (Sebastes auriculatus) occurs in nearshore habitat and is common from northern Baja California, Mexico to northern California, rare off the outer coast of Oregon and Washington and again common in the inland ...
... Many cotton (Gossypium hirsutum L.) pollination studies have been performed in the southern USA, but no data exist for California. In this study, we measured pollen-mediated gene flow (PGF) in four directions over 2 yr from herbicide-resistant source plots in upland cotton in the California cotton growing region and in a region with high pollinator activity. In addition, samples were taken from fi ...
... Pulsed-field gel electrophoresis and serotyping were performed for 544 isolates of Listeria monocytogenes, including 502 isolates recovered from contaminated samples from 31,705 retail ready-to-eat (RTE) food products and 42 isolates recovered from human cases of listeriosis. The isolates were from Maryland (294 isolates) and California (250 isolates) and were collected in 2000 and 2001. The isola ...
... Noninvasive faecal DNA sampling has the potential to provide a wealth of information necessary for monitoring and managing endangered species while eliminating the need to capture, handle or observe rare individuals. However, scoring problems, and subsequent genotyping errors, associated with this monitoring method remain a great concern as they can lead to misidentification of individuals and bia ...
loci; epidemiology; DNA primers; DNA; polymerase chain reaction; Didymella; Ascochyta rabiei; crossing; Ascochyta lentis; population structure; pathogens; fungi; California
Abstract:
... The mating type (MAT) locus of the lentil pathogen, Ascochyta lentis, was cloned and characterized using thermal asymmetric interlaced and inverse PCR with primers designed to the HMG-box of Ascochyta rabiei. A multiplex PCR assay for mating type was developed based on MAT idiomorph and flanking sequences. Primers were designed to specifically amplify MAT from several Ascochyta spp. including A. p ...
... Transgenic crops producing toxins from the bacterium Bacillus thuringiensis (Bt) kill insect pests and can reduce reliance on insecticide sprays. Although Bt cotton (Gossypium hirsutum L.) and Bt corn (Zea mays L.) covered 26 million ha worldwide in 2005, their success could be cut short by evolution of pest resistance. Monitoring the early phases of pest resistance to Bt crops is crucial, but it ...
DNA; arsenates; clones; denaturing gradient gel electrophoresis; genes; lakes; polymerase chain reaction; radionuclides; ribosomal RNA; salinity; sediments; sulfates; California
Abstract:
... A radioisotope method was devised to study bacterial respiratory reduction of arsenate in sediments. The following two arsenic-rich soda lakes in California were chosen for comparison on the basis of their different salinities: Mono Lake (~90 g/liter) and Searles Lake (~340 g/liter). Profiles of arsenate reduction and sulfate reduction were constructed for both lakes. Reduction of [73As]arsenate o ...
human diseases; humans; antibiotic resistance; ribotypes; Salmonella Typhimurium; bacteriophages; dairy cattle; plasmids; salmonellosis; pulsed-field gel electrophoresis; DNA fingerprinting; strains; microbial genetics; transposons; infection; California
Abstract:
... Fifty-six human and 24 adult dairy cattle isolates of Salmonella enterica serovar Typhimurium from a single county in California were compared using ribotyping, insertion sequence typing (IS200), pulsed-field gel electrophoresis, plasmid typing, phage typing, and antimicrobial resistance testing. The majority of the isolates fell into one of two groups which were phage types DT104 and DT193. Combi ...
... Various Salsola spp. have been introduced to the USA, and some of these are considered noxious or invasive in certain regions. The genus is taxonomically challenging, and recent morphological, cytological, and molecular work has shown that an unknown taxon, previously identified as S. tragus, but recently temporarily designated as Type B, with unknown origins, exists in California and Arizona. Typ ...
DNA; Zalophus; animals; emerging diseases; genotype; geographical variation; islands; major histocompatibility complex; California; Gulf of California; Pacific Ocean
Abstract:
... Major histocompatibility complex (MHC) class II DRB genotypes were examined in two geographically isolated populations of California sea lions (Zalophus californianus) (Gulf of California and California coastal Pacific Ocean). Genomic DNA from 227 California sea lions was examined using eight sequence-specific primer (SSP) pairs flanking the putative peptide-binding site. A total of 40 different Z ...
... I present the results of a culture-independent survey of soil bacterial communities from serpentine soils and adjacent nonserpentine comparator soils using a variety of newly developed phylogenetically based statistical tools. The study design included site-based replication of the serpentine-to-nonserpentine community comparison over a regional scale (~100 km) in Northern California and Southern ...
... Resolving conflicts between predators and livestock producers depends on obtaining reliable information about the predators that kill livestock. We used salivary DNA obtained from attack wounds on domestic sheep carcasses to identify the species of predator responsible for the kill, as well as the sex and individual identity of coyotes (Canis latrans) that killed sheep. Coyotes killed 36 of 37 dep ...
... We characterized the arsenate-reducing, sulfide-oxidizing population of Mono Lake, California, by analyzing the distribution and diversity of rrnA, cbbL, and dissimilatory arsenate reductase (arrA) genes in environmental DNA, arsenate-plus sulfide-amended lake water, mixed cultures, and isolates. The arsenate-reducing community was diverse. An organism represented by an rrnA sequence previously re ...
... The choice of detection method for phytopathogens can be critically important in determining the success or failure of pest regulation systems. We present an assay for Phytophthora ramorum that uses 5' fluorogenic exonuclease (TaqMan) chemistry to detect and quantify the pathogen from diseased tissue, and include a universal primer and probe set for an internal positive control. This method is sen ...
... A new method based on quantitative real-time polymerase chain reaction (qPCR) was developed and applied to quantify the red tide dinoflagellate Lingulodinium polyedrum in natural seawater samples and in laboratory cultures. The method uses a Molecular Beacon[trade mark sign] approach to target a species-specific region of the small subunit ribosomal RNA gene. The accuracy of the method was verifie ...
... Vibrio cholerae is an autochthonous member of diverse aquatic ecosystems around the globe. Collectively, the genomes of environmental V. cholerae strains comprise a large repository of encoded functions which can be acquired by individual V. cholerae lineages through uptake and recombination. To characterize the genomic diversity of environmental V. cholerae, we used comparative genome hybridizati ...
... Sixty-two questing adult Rhipicephalus sanguineus (Latreille) ticks were collected by direct removal from blades of turfgrass and adjacent concrete walkways at a suburban home in Riverside County, CA, and tested for the presence of Rickettsia, Bartonella, and Ehrlichia DNA. Polymerase chain reaction (PCR) was used to amplify fragments of the 17-kDa antigen gene and the rOmpA gene of the spotted fe ...
SCHWARTZ MICHAEL K.; AUBRY KEITH B.; MCKELVEY KEVIN S.; PILGRIM KRISTINE L.; COPELAND JEFFREY P.; SQUIRES JOHN R.; INMAN ROBERT M.; WISELY SAMANTHA M.; RUGGIERO LEONARD F.
history; species reintroduction; haplotypes; mitochondrial DNA; spatial distribution; phylogeny; wildlife habitats; population genetics; forest habitats; population dynamics; Gulo gulo; California
Abstract:
... Delineating a species' geographic range using the spatial distribution of museum specimens or even contemporary detection––non-detection data can be difficult. This is particularly true at the periphery of a species range where species' distributions are often disjunct. Wolverines (Gulo gulo) are wide-ranging mammals with discontinuous and potentially isolated populations at the periphery of their ...
Termopsidae; insect colonies; population structure; insect genetics; microsatellite repeats; loci; worker insects; soldier insects; population genetics; California
Abstract:
... Understanding the origin and maintenance of eusociality in termites has proved problematic, in part, due to a lack of knowledge concerning the variability and evolutionary changes in termite breeding structure. One way to address this is to compare the population genetics of a broad range of termite species. However, few studies have investigated the population genetics of basal termite taxa. We u ...
... The genetics of adaptation is a key problem in evolutionary biology. Pocket gophers of the species Thomomys bottae provide one of the most striking examples of coat color variation in mammals. Dorsal pelage color is strongly correlated with soil color across the range of the species, presumably reflecting the selective pressure exerted by predation. To investigate the genetic basis of coat color v ...
... Two strains of pink-colored aerobic bacteriochlorophyll a-containing bacteria were isolated from aerobic (strain ROS 10) and anaerobic (strain ROS 35) zones of the water column of Mono Lake (California, United States). Cells of the bacteria were nonmotile oval gram-negative rods multiplying by binary fission by means of a constriction. No intracellular membranes were detected. Polyphosphates and p ...
... We developed a real-time LC PCR assay to detect a 152 bp sequence in an uncharacterized region of the Blastocystis genome. The described assay detected 11 of 11 ATCC strains of Blastocystis from subtypes 1, 3, and 4. Three of three stool samples from Oregon and California military personnel that were negative for Blastocystis by an ova and parasite test as well as a conventional PCR assay were pos ...
DNA; cladodes; cultivar identification; cultivars; databases; expressed sequence tags; gene frequency; genetic variation; genotyping; germplasm; loci; microsatellite repeats; new variety; polymerase chain reaction; principal component analysis; California
Abstract:
... Simple sequence repeat (SSR) markers generated from expressed sequence tag (EST) sequences represent useful tools for genotyping and their development is relatively easy because of the public availability of EST databases. We report design and application of EST-SSRs to assess the level of genetic diversity among thirty-five asparagus cultivars and to fingerprint DePaoli, a new variety released by ...
... BACKGROUND AND AIMS: Seagrasses are important facilitator species in shallow, soft-bottom marine environments worldwide and, in many places, are threatened by coastal development and eutrophication. One narrow-leaved species (Zostera marina) and one wide-leaved species, variously designated as Z. marina, Z. pacifica or Z. asiatica, are found off the California Channel Islands and adjacent Californ ...
... Granulocytic anaplasmosis (GA), caused by Anaplasma phagocytophilum, is a potentially fatal, emerging rickettsial disease of humans, domestic animals, and wildlife. The purpose of this study was to determine whether sciurids from multiple areas of northern California were infested with ticks or exposed to or infected with A. phagocytophilum using polymerase chain reaction (PCR) and indirect-fluore ...
... Hydrothermal vents, known as 'fumaroles', are ubiquitous features of geothermal areas. Although their geology has been extensively characterized, little is known about the subsurface microbial ecology of fumaroles largely because of the difficulty in collecting sufficient numbers of cells from boiling steam water for DNA extraction and culture isolation. Here we describe the first collection, mole ...
... A growing number of ecologists are using molecular gut content assays to qualitatively measure predation. The two most popular gut content assays are immunoassays employing pest-specific monoclonal antibodies (mAb) and polymerase chain reaction (PCR) assays employing pest-specific DNA. Here, we present results from the first study to simultaneously use both methods to identify predators of the gla ...
... The copper rockfish is a benthic, nonmigratory, temperate rocky reef marine species with pelagic larvae and juveniles. A previous range-wide study of the population-genetic structure of copper rockfish revealed a pattern consistent with isolation-by-distance. This could arise from an intrinsically limited dispersal capability in the species or from regularly-spaced extrinsic barriers that restrict ...
... Landscape-scale population genetic structure in vagile vertebrates was commonly considered to be a contradiction in terms whereas recent studies have demonstrated behaviour and habitat associated structure in several such species. We investigate whether landscape features influence morphological and genetic differentiation in a widespread, mobile raptor. To accurately describe genetic differentiat ...
... Argasid ticks are vectors of viral and bacterial agents of humans and animals. Recent reports indicate that some ornithophilic argasids harbored rickettsial agents. A Nearctic tick, Argas monolakensis Schwan, Corwin, Brown is ornithophilic and has not previously been examined for rickettsial agents. Thirty adult A. monolakensis were tested by PCR for DNA from Rickettsia or Coxiella. Amplicons from ...
male genitalia; sequence analysis; insect morphology; interspecific variation; insect taxonomy; geographical distribution; haplotypes; Hesperiidae; mitochondrial DNA; nuclear genome; molecular systematics; butterflies; phylogeography; genetic variation; Sierra Nevada (California); California
Abstract:
... We examined patterns of genetic variation between the transcontinental species Pyrgus communis (Grote) and Pyrgus albescens Plötz (Hesperiidae) to examine whether patterns of molecular variation are congruent with the taxonomy. Sequence data from mitochondrial DNA and nuclear DNA failed to distinguish the two taxa. Although substantial genetic variation is explained by the two nominal taxa, more ...
DNA; Laridae; aquatic communities; bacteria; bacterial communities; digestive system; feces; genes; mammals; molecular cloning; phylogeny; polymerase chain reaction; public health; ribosomal RNA; water pollution; waterfowl; California; Canada; Georgia; Lake Erie; Lake Michigan; Lake Ontario; Ohio; Wisconsin
Abstract:
... In spite of increasing public health concerns about the potential risks associated with swimming in waters contaminated with waterfowl feces, little is known about the composition of the gut microbial community of aquatic birds. To address this, a gull 16S rRNA gene clone library was developed and analyzed to determine the identities of fecal bacteria. Analysis of 282 16S rRNA gene clones demonstr ...
... We tested for genetic differentiation among six populations of California sea mussels (Mytilus californianus) sampled across 4000 km of its geographical range by comparing patterns of variation at four independent types of genetic markers: allozymes, single-copy nuclear DNA markers, and DNA sequences from the male and female mitochondrial genomes. Despite our extensive sampling and genotyping effo ...
... In two recent studies, clinical isolates in the Fusarium solani species complex (FSSC) were sequenced; one of the most common lineages was FSSC Group 1 (FSSC 1), a phylogenetic species that is synonymous with F. solani f. sp. cucurbitae race 2, a pathogen of cucurbit fruits. FSSC 1 was also identified in sink and shower drains in two hospitals. The environmental sources of FSSC 1 are important for ...
... We have collected evidence that the nominal species, Polydora cornuta B osc 1802, contains at least three separate species in North America. Specimens of P. cornuta were collected in California, Florida, and Maine, raised in the laboratory, and assessed for reproductive compatibility, genetic similarity, gamete characteristics, and developmental rates. Reproductive crosses between each combination ...
... This study sought to evaluate the distribution of the enterococcal surface protein (esp) gene in Enterococcus faecium in the Pacific coast environment as well as the distribution and diversity of the gene in Northern California animal hosts. Over 150 environmental samples from the Pacific coast environment (sand, surf zone, fresh/estuarine, groundwater, and storm drain) were screened for the esp g ...
... The Cow Head Tui Chub, Siphateles thalassinus vaccaceps, is restricted to the small Cow Head basin in far northeastern California and northwestern Nevada. Using microsatellite DNA loci, we found extensive genetic variation among tui chub populations in the Cow Head and surrounding basins of the northwestern Great Basin. Our data support the systematic conclusions of earlier authors, while showing ...
... A species of aster leafhopper (Macrosteles sp.) became established in 2001 on Oahu, Hawaii, and through the transmission of the aster yellows phytoplasma, caused devastating losses to the island's watercress industry. DNA sequence data were analysed from two mitochondrial genes [cytochrome oxidase subunit 1(CO1) and nicotinamide adenine dinucleotide 1 (NADH1)] and one nuclear gene (wingless, Wg) ( ...
Preston, Christina M.; Marin, Roman III.; Jensen, Scott D.; Feldman, Jason; Birch, James M.; Massion, Eugene I.; DeLong, Edward F.; Suzuki, Marcelino; Wheeler, Kevin; Scholin, Christopher A.
Cyanobacteria; DNA; DNA probes; alpha-Proteobacteria; bacterioplankton; buffers; detection limit; equipment; homogenization; molecular cloning; ribosomal RNA; time series analysis; California
Abstract:
... A sandwich hybridization assay (SHA) was developed to detect 16S rRNAs indicative of phylogenetically distinct groups of marine bacterioplankton in a 96-well plate format as well as low-density arrays printed on a membrane support. The arrays were used in a field-deployable instrument, the Environmental Sample Processor (ESP). The SHA employs a chaotropic buffer for both cell homogenization and hy ...
Crustacea; DNA; DNA barcoding; adults; endangered species; genotyping; habitats; indigenous species; life history; reproduction; shrimp; taxonomic keys; vernal pools; California
Abstract:
... The San Diego fairy shrimp Branchinecta sandiegonensis is a federally endangered species endemic to vernal pools in southern California, USA. Filling events in these habitats are highly variable, with some pools failing to hold water long enough for reproduction over many successive years. Studies of this species are thus hindered by the relatively rare appearance of aquatically active life histor ...
DNA; Fusarium oxysporum f. sp. ciceri; avirulent strains; chickpeas; genome; heterokaryon; monophyly; races; random amplified polymorphic DNA technique; roots; virulence; Algeria; California; India; Italy; Morocco; Pakistan; Spain; Tunisia
Abstract:
... Forty-seven isolates of Fusarium oxysporum f.sp.ciceris, representative of the different pathogenic races and originating from California, India, Tunisia and Spain, and twenty-five non-pathogenic F. oxysporum isolates obtained from chickpea roots in Algeria, Italy, Morocco, Pakistan and Spain, were analyzed for vegetative compatibility. Only one vegetative compatibility group (VCG) was found among ...
Angiospermae; DNA; Polyphylla; endangered species; ferns and fern allies; frass; host plants; insects; microhabitats; palate; vegetation; California
Abstract:
... Precise information about endangered species, in particular identifying their resources requirements, is needed to identify areas that might support populations. Little is known about the endangered Mount Hermon June Beetle (Polyphylla barbata) found only within Zayante soils region of Santa Cruz County, California. We investigated the beetle's host plant selection, habitat association and mating ...
... Detection of Phytophthora ramorum in US commercial nurseries has led to a number of quarantine regulations. Methods such as real-time PCR (RT-PCR) provide rapid and reliable detection that can supplement attempts to culture P. ramorum from symptomatic tissue. We adapted and optimized a previously described mitochondrial gene-based RT-PCR assay for use with a Cepheid SmartCycler v.1 and ready-to-us ...
... Red-pigmented biofilms grow on rock and cobble surfaces present in anoxic hot springs located on Paoha Island in Mono Lake. The bacterial community was dominated (~ 85% of 16S rRNA gene clones) by sequences from the photosynthetic Ectothiorhodospira genus. Scraped biofilm materials incubated under anoxic conditions rapidly oxidized As(III) to As(V) in the light via anoxygenic photosynthesis but co ...
... Marine hydrocarbon seeps supply oil and gas to microorganisms in sediments and overlying water. We used stable isotope probing (SIP) to identify aerobic bacteria oxidizing gaseous hydrocarbons in surface sediment from the Coal Oil Point seep field located offshore of Santa Barbara, California. After incubating sediment with ¹³C-labeled methane, ethane, or propane, we confirmed the incorporation of ...
mitochondrial DNA; phylogeny; Frankliniella occidentalis; genes; population dynamics; genetic markers; plant pests; insect genetics; insect ecology; sequence analysis; geographical distribution; insect pests; barcoding; cytochrome P-450; loci; geographical variation; population genetics; nucleotide sequences; genetic variation; pest monitoring; California
Abstract:
... Over the past three decades, Western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), has become a major worldwide pest of many agricultural and horticultural crops. In response, much time, money, and effort have been put into pure and applied research focusing on the biology and control of this pest, Western flower thrips is native to Western North America and wides ...
... Cats are the main reservoirs of zoonotic Bartonella henselae, B. clarridgeiae and B. koehlerae, transmitted among cats by cat fleas. No study has investigated the presence of Bartonella in the saliva of bacteremic and non-bacteremic cats to correlate it to the level of bacteremia and the presence or absence of oral lesions. Shelter cats from northern California (n =130) and Michigan (n =50) were t ...
life history; phylogeny; genes; phylogeography; Tilopteridales; gametophytes; haplotypes; open reading frames; DNA; males; genetic distance; sex ratio; females; genetic relationships; parthenogenesis; germ cells; genetic variation; Korean Peninsula; Mexico; California; Japan
Abstract:
... Cutleria cylindrica Okamura was described from Japan in 1902 and has been reported only from northwestern Asia until its relatively recent discovery in California, USA, and Baja California, Mexico. To clarify the genetic relationships within and among the disjunct populations, we carried out a molecular phylogenetic study, as well as the examination of sex ratio and the life-history patterns, of p ...
... Background: Salmon poisoning disease (SPD) is a trematode-borne disease of dogs caused by Neorickettsia helminthoeca. Objectives: To determine risk factors and spatial epidemiology of SPD in dogs from northern California; to describe the clinicopathologic, microbiologic, and imaging findings of SPD in these dogs; and to evaluate treatments and outcomes for SPD. Animals: Twenty-nine dogs with SPD b ...
... Aims: Vibrio cholerae is an important bacterial pathogen that causes global cholera epidemic. Although they are commonly found in coastal waters around the world, most environmental isolates do not contain cholera toxin genes. This study investigates vibriophages in southern California coastal waters and their ability to transfer cholera toxin genes. Methods and Results: Lytic phages infecting V. ...
... A molecular assay for diagnosis of light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae), in North America is reported. The assay multiplexes two TaqMan real-time polymerase chain reaction (RT-PCR) probe systems that are designed to target DNA segments of the internal transcribed spacer region 2 (ITS2) and 18S rRNA gene. The RT-PCR probe designed for the 18S target recog ...
Amanita; DNA; fruiting; new species; phenotype; sequence analysis; California
Abstract:
... We describe Amanita vernicoccora, a vernal fruiting species known as the "spring coccora" in California. Sequence analyses of four DNA regions and phenotypic traits demonstrate that A. vernicoccora, long considered a pale-colored form of the autumnal fruiting A. calyptroderma (the 'fall coccora'), is a unique species. Morphological and genetic data support both species in section Caesareae. We als ...
... In California, perennial pepperweed (Lepidium latifolium) is an introduced Brassicaceae plant that is invasive, highly competitive, and listed as a noxious weed that grows in areas such as marshes, meadows, roadsides, and irrigation ditches. From 2008 through 2010, perennial pepperweed growing near farms in Monterey and Santa Clara counties was infected with white rust. Symptoms were light green-t ...
... The soft tick, Ornithodoros coriaceus (Koch) (Acari: Argasidae), is a common mammalian parasite of livestock in many arid regions of the western U.S.A. The tick is a known vector of the undescribed bacterial pathogen that causes epizootic bovine abortion (EBA), which results in late-term abortions in beef cattle and subsequent economic loss, which can be considerable, to producers. A second report ...
... Fire blight of pear and apple is frequently an inoculum-limited disease but weather-based forecasting models commonly assume that the pathogen is omnipresent. To improve fire blight risk assessment during flowering, we developed a rapid pathogen detection protocol that uses loop-mediated isothermal amplification (LAMP) to detect DNA of epiphytic Erwinia amylovora on samples of pear and apple flowe ...
... Infections by Puccinia psidii Winter were detected on Melaleuca quinquenervia (Cav.) Blake (paperbark, tea tree) during a regular inspection of a San Diego, California nursery in November 2010 by San Diego County inspectors. Urediniospore morphology, production of bright-yellow uredinia on both upper and lower leaf surfaces, DNA sequencing, and inoculation tests confirm the identity of the pathoge ...
Symbiodinium; clones; coral reefs; environmental factors; founder effect; gene frequency; genetic variation; genotype; microsatellite repeats; space and time; symbionts; symbiosis; Gulf of California
Abstract:
... Genetic data are rapidly advancing our understanding of various biological systems including the ecology and evolution of coral–algal symbioses. The fine‐scale interactions between individual genotypes of host and symbiont remain largely unstudied and constitute a major gap in knowledge. By applying microsatellite markers developed for both host and symbiont, we investigated the intracolony divers ...
... The screening of environmental DNA metagenome libraries for functional activities can provide an important source of new molecules and enzymes. In this study, we identified 17 potential protease-producing clones from two metagenomic libraries derived from samples of surface sand from the Gobi and Death Valley deserts. Two of the proteases, DV1 and M30, were purified and biochemically examined. The ...
Bartonella; DNA; Rattus norvegicus; Xenopsylla cheopis; rats; taxonomy; California
Abstract:
... Of 200 individual Xenopsylla cheopis fleas removed from Rattus norvegicus rats trapped in downtown Los Angeles, CA, 190 (95%) were positive for the presence of Bartonella DNA. Ninety-one amplicons were sequenced: Bartonella rochalimae-like DNA was detected in 66 examined fleas, and Bartonella tribocorum-like DNA was identified in 25 fleas. The data obtained from this study demonstrate an extremely ...
DNA; Mastocarpus; algae; chloroplasts; cryptic species; cytochrome-c oxidase; genome; mitochondria; new species; phylogeny; California
Abstract:
... Lindstrom S.C., Hughey J.R. and Martone P.T. 2011. New, resurrected and redefined species of Mastocarpus (Phyllophoraceae, Rhodophyta). Phycologia 50: 661–683. DOI: 10.2216/10-38.1 Recent molecular phylogenetic investigations of the red algal genus Mastocarpus from the northeast Pacific resolved numerous cryptic species. Although species were clearly defined through genetic analyses, the correct n ...
hybrids; amplified fragment length polymorphism; DNA; phenotypic variation; genetic polymorphism; germplasm; lettuce; breeding; Lactuca; Arizona; Iowa; Wisconsin; Utah; California; Nevada; South Dakota; Idaho; Wyoming; Minnesota; Ontario; Quebec; Oregon; New York; Montana; North Carolina; Colorado
Abstract:
... In the years 2002-2008, missions were undertaken in the USA and Canada to search for wild and weedy Lactuca species. Altogether, 16 states in the USA (Arizona, California, Colorado, Idaho, Iowa, Minnesota, Montana, Nevada, New York, North Carolina, Oregon, South Dakota, Utah, Washington, Wisconsin and Wyoming) and two provinces in Canada (Ontario and Quebec) were visited. In total, seven wild and ...
germplasm; plateaus; ecoregions; rangelands; seed industry; DNA; mountains; Dalea ornata; land restoration; genetic variation; new species; forbs; Oregon; Idaho; Nevada; Snake River; California
Abstract:
... Two natural-track selected germplasms of western prairie clover (Dalea ornata (Douglas ex Hook.) Eaton & J. Wright [Fabaceae]) have been released for use in revegetation of semiarid rangelands in the western US. Western prairie clover is a perennial leguminous forb that occurs naturally in Idaho, Washington, Oregon, California, and Nevada. Majestic Germplasm western prairie clover originates from ...
... The hemoplasmas are the trivial name for a group of erythrocyte-parasitizing, non-cultivable in vitro bacteria of the genus Mycoplasma that have been described in several mammalian hosts worldwide. This study is the first report of hemoplasmas in marine mammals. EDTA anticoagulated whole blood samples from 137 California sea lions (Zalophus californianus) and 20 northern elephant seals (Mirounga a ...