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... Adding alkaline into an anaerobic waste activated sludge (WAS) fermentation with thermophilic bacteria pretreatment could efficiently improve short-chain fatty acids (SCFAs) accumulation to 3550 ± 120 mg COD/L. The acidification rate in combined test was 21.2%, while that was 15.6% and 10.7% in sole thermophilic bacteria pretreatment and control tests respectively. Four distinct groups of microbes ...
... Effect of rumen microorganisms on hydrolysis of food waste in leach bed reactor (LBR) was investigated. LBRs were inoculated (20%, w/w) with cow manure and anaerobically digested sludge at different ratios, 0:1 (LBR-A), 1:3 (LBR-B), 1:1 (LBR-C), 3:1 (LBR-D) and 1:0 (LBR-E). High volatile solids (VS) conversion efficiency of 68% was achieved in LBR-E. Compared with LBR-A, chemical oxygen demand, to ...
... Previous studies have shown that biochar enhances microbial reduction of iron (Fe) oxyhydroxide under anaerobic incubation. However, there is a lack of data on its influence on arsenic (As) release from As-contaminated paddy soils. In this study, paddy soil slurries (120 mg As kg⁻¹) were incubated under anaerobic conditions for 60 days with and without the addition of biochar (3%, w/w) prepared fr ...
... Lignocellulosic residues used for biofuel production is an alternative source to increase the energy supply. However, the cellulose found in this biomass must be made available in fermentable sugars, requiring a complex enzymatic mechanism only found in specific microorganisms. Some thermophilic and anaerobic bacteria of the Clostridium species are able to produce cellulolytic enzymes and metaboli ...
... A novel moderately thermophilic, anaerobic, ethanol-producing bacterial strain, 45BT, was isolated from a mixed sediment water sample collected from a hot spring at Potosi, Bolivia. The cells were straight to slightly curved rods approximately 2.5 µm long and 0.5 µm wide. The strain was Gram-stain-variable, spore-forming and monotrichously flagellated. Growth of the strain was observed at 45–65 °C ...
... The Pathology of digestive tract has long been known to be correlated with chronic kidney disease (CKD). The member of the major Firmicutes phylum especially Clostridium subcluster XIVa altered quantitatively and qualitatively in the gut microbiota of patients with End Stage Renal Disease (ESRD) and CKD. Therefore, in this study, the abundance of the species of Clostridium genus of Firmicutes phyl ...
Tatiana A. Vishnivetskaya; Scott D. Hamilton-Brehm; Mircea Podar; Jennifer J. Mosher; Anthony V. Palumbo; Tommy J. Phelps; Martin Keller; James G. Elkins
... The conversion of lignocellulosic biomass into biofuels can potentially be improved by employing robust microorganisms and enzymes that efficiently deconstruct plant polysaccharides at elevated temperatures. Many of the geothermal features of Yellowstone National Park (YNP) are surrounded by vegetation providing a source of allochthonic material to support heterotrophic microbial communities adapt ...
Aminobacterium; Caloramator; Clostridium; Lactobacillus; Methanobacteria; Methanoculleus; Methanosarcina; Ruminococcus; Syntrophomonas; community structure; fermentation; functional diversity; genes; methanogens; microbial communities; taxonomy; China
Abstract:
... PURPOSE: The microbial community in the pit mud correlated closely with the quality of the final product of Chinese strong-flavored Baijiu (CSFB). However, environmental conditions and brewing processes can vary by region and distilleries. This may lead to differences in microbial composition and function in pit mud. Therefore, revealing the features of the pit mud microbial community structure an ...
... The pit mud microbes are tightly correlated with the quality of Chinese strong aroma-type Baijiu. However, it is still unclear the explicit contributions of pit mud microbes to flavour formation. Through down-scaled fermentation with or without pit mud, we found that 85 genera from pit mud, dominating by Caproiciporducens, Caloramator, Sedimentibacter and Caldicoprobacter, migrated into fermented ...
... A novel pretreatment method combining ultrasonic with thermophilic bacteria (Geobacillus sp. G1) was employed to pretreat waste-activated sludge (WAS) for enhancing the WAS hydrolysis and subsequent volatile fatty acids (VFAs) production. The soluble protein and carbohydrate were mostly released from intracellular ultrasonic-assisted Geobacillus sp. G1 pretreatment, and accumulated to 917 ± 70 and ...
... To explore diversity of thermophilic exoelectrogens, a thermophilic microbial fuel cell was constructed. Population analysis of the anodic microorganisms suggested possible involvement of Caloramator-related bacteria in electricity generation. Pure culture of Caloramator australicus showed electricity-generating ability, indicating that the bacterium is a new thermophilic exoelectrogen. ...
... The recently isolated anaerobic bacterium Caloramator boliviensis with an optimum growth temperature of 60°C can efficiently convert hexoses and pentoses into ethanol. When fermentations of pure sugars and a pentose-rich sugarcane bagasse hydrolysate were carried out in a packed bed reactor with immobilized cells of C. boliviensis, more than 98% of substrates were converted. Ethanol yields of 0.40 ...
... This study investigated a previously developed thermophilic microbial community with the ability to effectively degrade azo dyes. To identify the key microbes of this microbial community, a dilution-to-extinction approach was combined with polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and Illumina high-throughput sequencing technology (HTST). Strains belonging to Tep ...
Acinetobacter; Arcobacter; Bacteroides; Caloramator; Clostridium; Flavobacterium; Opitutus; Pseudomonas; Streptomyces; metagenomics; microbiome; multivariate analysis; pH; pollution; public health; riparian areas; risk; river water; rivers; salinity; sewage; temperature; variance; India
Abstract:
... Microbial communities are considered as vital members to reflect the health of a riverine system. Among them, pathogenic and fecal indicators imply health risks involved with potability of river water. The present study explores the diverse microbial communities, distribution pattern of potential pathogens, and fecal indicators between the geographically distinct Himalayan and Peninsular river sys ...
... A novel pretreatment method combining freezing/thawing with Geobacillus sp. G1 was employed to pretreat waste activated sludge (WAS) for enhancing the WAS hydrolysis and subsequent short-chain fatty acids (SCFAs) production. Results showed that freezing/thawing combined with Geobacillus sp. G1 pretreatment achieved the maximal concentrations of soluble protein from 40±6mg COD/L (non-pretreated) to ...
... This study evaluated the influence of a high organic loading rate (OLR) on thermophilic hydrogen production at an up-flow anaerobic packed-bed reactor (APBR) treating a residual liquid stream of a Brazilian biorefinery. The APBR, filled with low-density polyethylene, was operated at an OLR of 84.2kg-CODm−3d−1. This value was determined in a previous study. The maximum values of hydrogen production ...
... The thermoanaerobe, Caloramator boliviensis was used to ferment starch hydrolysate from inedible wild cassava to ethanol at 60°C. A raw starch degrading α-amylase was used to hydrolyse the cassava starch. During fermentation, the organism released CO2 and H2 gases, and Gas Endeavour System was successfully used for monitoring and recording formation of these gaseous products. The bioethanol produc ...
... A thermophilic anaerobic bacterium was isolated from a sublacustrine hydrothermal vent site in Lake Tanganyika (East Africa) with recorded fluid temperatures of 66–103°C and pH values of 7.7–8.9. The bacterium (strain TR10) was rod-shaped, about 1 by 5μm in size, and readily formed distal endospores. Based on the 16S rDNA sequence the novel strain was homologous to Thermobrachium celere and Calora ...
... Fe(III) oxides have been investigated to accelerate anaerobic methanogenic degradation of complex organic compounds. However, the critical role linked to the characteristics of different types of Fe(III) oxides is still unclear. Study presented here performed a side-by-side comparison of four types of Fe(III) oxides including Fe(III)-citrate, ferrihydrite, hematite and magnetite to evaluate their ...
... Application of plant growth promoting rhizo-bacteria (PGPR) is a promising method for sustainably increasing the plant growth; however, survival of PGPR is the most important factor limiting its efficiency. We studied the effects of heterotrophic protists on the survival of introduced PGPR, Azospirillum sp. B510, on indigenous bacterial community structure, and on the growth of the rice (Oryza sat ...