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- Yu, Sungryul; Park, Jeong Soo; Paredes, Verenice; Song, Myoung-Chong; Baek, Nam-In; Lee, Sang-Ik; Lim, Jong-Soon; Cho, Nam-Young; Yoon, Jaeseung; Baek, Kwanghee
- Journal of biotechnology 2010 v.145 no.3 pp. 304-309
- Gleditsia sinensis, etc ; Chinese hamsters; agonists; animal ovaries; antagonists; biotechnology; dopamine; dose response; fractionation; humans; medicinal plants; plant extracts; screening; Show all 13 Subjects
- ... To develop a cell-based assay to screen for human dopamine D₁ receptor agonists or antagonists from medicinal plant extracts, a stable Chinese hamster ovary (CHO) cell line (CHO-D1R) expressing the human dopamine D₁ receptor was established using an expression vector containing a scaffold attachment region (SAR) element. CHO-D1R cells showed specific binding to [³H]-SCH23390 with high affinity (K ...