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... Fruit and juice characteristics of seven pummelo cultivars, namely 'Khao Hom', 'Khao Nam Pleung', 'Khao Paen' 'Khao Phaung', 'Khao Taeng-kwa', 'Takoy' and 'Tongdee' grown under Thai conditions were comparatively studied. Fruit weight, fruit size, specific gravity, number of seeds per fruit, carpels and oil glands, peel thickness, color of albedo and juice sacs and firmness of juice sacs were deter ...
random amplified polymorphic DNA technique; Ficus benjamina; chemical constituents of plants; DNA; Citrus maxima; extraction; polysaccharides; Liliaceae
... The effects of variety, rootstock, and geographical location were studied as to their effects on secondary metabolite production in grapefruit and pummelo. The concentration of the flavanones narirutin, naringin, and neohesperidin and of the sesquiterpene nootkatone, which is principally responsible for the grapefruit's aroma, varies during fruit development. The highest flavanone levels are detec ...
pummelos; Citrus maxima; essential oils; food composition; gas chromatography; physicochemical properties; chemical constituents of plants; high performance liquid chromatography; Italy
Abstract:
... The qualitative and quantitative composition of volatile and nonvolatile fraction of pummelo (Citrus grandis (L.) Osbeck) essential oil is reported. The oil was analyzed by GC, HPLC and GC/MS. Limonene (93.83%) was the major constituent. ...
interspecific variation; geranial; neral; beta-pinene; Citrus aurantiifolia; essential oils; beta-caryophyllene; citronellal; limonene; Citrus maxima; chemical constituents of plants; leaves; Malaysia
Abstract:
... The chemical composition of the peel and leaf oils of four Malaysian Citrus species (C. hystrix D.C., C. aurantifolia Swingle, C. maxima Merr. and C. microcarpa Bunge) was examined by co-chromatography, two columns of different polarity, GC/MS and linear retention indices. beta-Pinene and limonene were the major constituents of the peel oils of C. hystrix (39.3% and 14.2%) and C. aurantifolia (28. ...
random amplified polymorphic DNA technique; hybrids; DNA methylation; loci; restriction mapping; interspecific hybridization; linkage groups; restriction endonucleases; cytosine; Citrus maxima; Poncirus trifoliata; genetic polymorphism; genetic markers
Abstract:
... A new method was developed to detect DNA methylation in the Citrus genome using random amplification coupled with restriction enzyme digestion. Genomic DNA from Citrus grandis (L.) Osb., Poncirus trifoliata (L.) Raf., and their F1 hybrid was amplified using 7 individual 10-mer random primers. Prior to amplification the DNA templates were digested with 2 pairs of restriction endonucleases (HpaII-Ms ...
Citrus maxima; acidity; genetic markers; pH; citric acid; recessive genes; nucleotide sequences; fruit juices; restriction fragment length polymorphism; random amplified polymorphic DNA technique; chemical constituents of plants; linkage (genetics)
Abstract:
... Fruit juice pH, titratable acidity, or citric acid content was measured in 6 populations derived from an acidless pummelo (pummelo 2240) (Citrus maxima (Burm.) Merrill). The acidless trait in pummelo 2240 is controlled by a single recessive gene called acitric. Using bulked segregant analysis, three RAPD markers were identified as linked to acitric. RAPD marker OpZ20(410), which mapped 1.2 cM from ...
... A possible way to valorize citrus peels and seeds, which are byproducts of the juice extraction industry, is to use them as natural antioxidants. The antioxidant activity of several citrus peel and seed extracts obtained either by methanol extraction (free phenolic compounds) or by alkaline hydrolysis (bound phenolic compounds) was tested in a model system based on accelerated citronellal oxidatio ...
... Many of the world’s most important citrus cultivars (”Washington Navel”, satsumas, clementines) have arisen through somatic mutation. This phenomenon occurs fairly often in the various species and varieties of the genus.The presence of copia-like retrotransposons has been investigated in fruit trees, especially citrus, by using a PCR assay designed to detect copia-like reverse transcriptase (RT) s ...
... The effect of waxes on the mobilities of organic solutes in isolated leaf cuticular membranes (CM) of six species was investigated using compounds with molar volumes ranging from 99 cm³ mol⁻¹ (salicylic acid) to 349 cm³ mol⁻¹ (cholesterol). When log(solute mobility) was plotted versus molar volumes straight lines were obtained for all plant species showing that the mobilities decreased exponential ...
... The effects of salinization with 40 mM NaCl on Poncirus trifoliata (L.) Raf., Citrus grandis (L.) Osb., their F(1), and a BC(1) progeny population (C. grandis x (F(1))) were investigated by means of Na(+) and Cl(-) analyses and QTL (quantitative trait loci) mapping. A total of 38 traits related to different tissue or whole-plant Na(+) and (or) Cl(-) accumulation was analyzed in salinized and nonsa ...
... Several Citrus species are cultivated in Táchira, an Andean State of Venezuela. Fresh fruits are sold as such, or processed to produce juice, marmalade, sauce and as additive in children’s food. The purpose of this work was to determine and compare the composition of the essential oils from the pericarp of Citrus paradisi (red and white varieties), C. grandis, C. limon and C. reticulata. Fruits we ...
... An enzyme that catalyses the transfer of a glucose unit from UDPG to limonoids was isolated from the albedo tissue of pummelo fruit (Citrus grandis Osbeck) in electrophoretically homogeneous form. The enzyme was purified to 180-fold by a combination of (NH4)2SO4 fractionation, ion exchange chromatography on DEAE-cellulose and DEAE-Toyopearl. SDS-PAGE showed a molecular weight of 55 kDa for the enz ...
in vitro regeneration; 4-CPA; in vitro culture; indole butyric acid; pummelos; culture media; Citrus maxima; leaves; micropropagation; plant morphology; naphthaleneacetic acid; 2,4,5-T; 2,4-D; fruit trees; callus
Abstract:
... Calli were induced from leaf explants of seedling in Citrus grandis (L.) Osbeck (pummelo) on MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthaleneacetic acid (NAA), 2,4,5-trichlorophenoxyacetic acid, 2-methyl-4-chlorophenoxyacetic, 4-chlorophenoxyacetic acid, 4-methoxy-3,6-dichlorobenzoic acid or 4-amino-3,5,6-trichloropicolinic acid. 2,4-D was most effective. Only gree ...
Citrus maxima; DNA; Poncirus trifoliata; analysis of variance; analytical methods; chromosome mapping; genetic markers; probability; progeny; quantitative trait loci; random amplified polymorphic DNA technique; t-test; temperature
Abstract:
... Mapping quantitative trait loci (QTL) associated with freeze tolerance was accomplished using a Citrus grandis (L.) Osb. × Poncirus trifoliata (L.) Raf. F(1) pseudo-testcross population. A progeny population of 442 plants was acclimated and exposed to temperatures of -9 °C and -15 °C in two separate freeze tests. A subpopulation of 99 progeny was genotyped for random amplified polymorphic DNA (RAP ...
... The use of morpholine in fruit coatings in the U.S. is common, however ammonia can be used instead. Resin coatings were made from aqueous ammonia solutions of shellac and wood rosin. The affect of rosin coatings on internal gas concentrations was found to be highly variable, depending on the amount of plasticizer added---thus opening the possibility of developing a rosin coating of citrus fruit th ...
... From February 1998 to May 2000, fruits of different citrus varieties and hybrids were collected in order to assess the fruit fly infestation levels and tephritoid/parasitoid complex in the state of Sao Paulo. A total of 12,239 fruits (1,416.93 kg) was collected in 25 municipalities. From all the samples 5,252 puparia and 3,039 adults of Tephritoidea were recovered. About 78.1% of all adults collec ...
... Infection with Likubin bacterium (LB) followed by Phytophthora parasitica increased the mortality of sour orange and pummelo seedlings, and enhanced the P. parasitica-induced root rot in all the four types of citrus tested. The LB-induced enhancement of root infection by P. parasitica was apparent within 1 h of exposure to zoospore suspension. The enhancement of P. parasitica-induced root rot was ...
... Several types of compounds with immunoreactivity similar to isoflavonoids were detected in water: ethanol extracts of leaves of Fortunella obovata Hort. ex Tanaka, Murraya paniculata Jack. and four Citrus species, namely C. aurantium L., C. grandis Osbeck, C. limonia Osbeck., and C. sinensis Osbeck (Rutaceae). The chromatographic mobilities of the immunoreactive substances were compared with those ...
biosynthesis; phytochemicals; plant extracts; biochemical pathways; secondary metabolites; chemical structure; Citrus maxima; reversed-phase high performance liquid chromatography; spectral analysis; limonin; limonoids; pummelos; United States
Abstract:
... A method combining solid-phase extraction and reversed-phase high-performance liquid chromatography is described for the isolation of two key metabolites in the limonoid biosynthetic pathway critical to citrus quality. Potassium salts of limonoate A-ring lactone and nomilinoate A-ring lactone were isolated from young Chandler pummelo seedlings and characterized on the basis of proton and carbon NM ...
... CMS (cytoplasmic male sterility) can be controlled by the mitochondrion genome in higher plants, including Satsuma mandarin. Somatic fusion experiments in citrus combining embryogenic callus protoplasts of one parent with leaf protoplasts of a second parent often produce cybrid plants of the leaf parent, a phenomenon occurring most often with interspecific fusion combinations. In an attempt to pra ...
acetone; citrus peels; plant biochemistry; quantitative analysis; psoralen; chemical analysis; plant extracts; high performance liquid chromatography; Citrus maxima; solvents; chemical structure; supercritical fluid extraction
Abstract:
... The efficiency of carbon dioxide supercritical fluid extraction (SFE) of the biologically active compounds imperatorin, meranzin and meranzin hydrate from the fruit peel of Citrus maxima Merr. has been compared with that of solvent extraction with acetone. Under the best SFE conditions tested for the three coumarins, which involved extraction at 50 degrees C and 27.6 MPa, the extractive efficienci ...
... BACKGROUND AND AIMS: The purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of intermediate oily seeds using Citrus as a model. METHODS: The relationships between equilibrium relative humidity (RH), seed water content, presence of freezable water as determined by DSC analysis, and germination percentage after immersion in liquid nitrogen (LN) we ...
... This study determined existing quantitative stand structure and its implication on silvicultural management of homestead forestry. The results showed that fruit and timber species have importance values of 57% and 43%, respectively, in the study area, which is in contrast to the commonly held view of absolute domination of fruit species. The fruit species were only moderately dominant over timber ...
essential oils; Citrus maxima; pummelos; Citrus paradisi; grapefruits; citrus peels; chemical composition; gas chromatography; mass spectrometry; Kenya
Abstract:
... The volatile constituents of cold-pressed peel essential oils of redblush grapefruit (Citrus paradisi Macfadyen forma Redblush) and pummelo (Citrus grandis Osbeck) from the same locality in Kenya were determined by GC and GC-MS. A total of 67 and 52 compounds, amounting to 97.9 and 98.8% of the two oils, respectively, were identified. Monoterpene hydrocarbons constituted 93.3 and 97.5% in the oils ...
... Twenty-four simple sequence repeat (SSR) markers were used to detect molecular polymorphisms among 370 mostly sexually derived Citrus accessions from the collection of citrus germplasm maintained at the University of California, Riverside. A total of 275 alleles were detected with an average of 11.5 alleles per locus and an average polymorphism information content of 0.625. Genetic diversity stati ...
... With growing use of synthetic adjuvants in modern agriculture, their impacts on the environment are being questioned. In a search for an environmentally safe phyto-adjuvant, we have investigated natural glycosidic saponin for delivery of agromaterials through plant cuticle membranes. Four saponin preparations from Quillaja saponaria bark (QE), obtained from Sigma-Aldrich, and Balanites aegyptiaca ...
... Plant cuticular materials play an important role in sorption of polar and nonpolar pollutants in soils. The objective of this study was to examine the effect of decomposition and transformation of plant cuticles on their sorption behavior with triazine herbicides and polycyclic aromatic hydrocarbons (PAHs). Sorption-desorption behavior was studied during 12 mo of incubation of cuticles isolated fr ...
... The antioxidative phytochemicals in various fruits and vegetables are widely recognized for their role in scavenging free radicals, which are involved in the etiology of many chronic diseases. Colored fruits are especially considered a quality trait that correlates with their nutritional values and health benefits. The specific aim of this study was to investigate the antioxidants in the juice and ...
... The nutritive values of leaves of Citrus grandis, Citrus aurantium, Citrus oranges, Citrus limon, and Citrus deliciosa were evaluated by chemical composition and in vitro gas production techniques. There were significant (p<0.001) differences among citrus species in terms of chemical composition. Crude protein (CP) contents ranged from 123.0 to 148.3 g/kg DM. Neutral detergent fibre (NDF) and acid ...
... Reduction of migration of fecal coliforms (FC) and streptococci (FS) by limiting the leaching in effluent-irrigated soil was tested in lysimeters packed with quartz sand without or with added biosolids compost or with one of two clayey soils. The 200-L, 70-cm-deep lysimeters were either planted with a Eucalyptus camaldulensis or an Oroblanco citrus tree (in the sand only), or not planted. The Euca ...
... Seedlings of Citrus grandis (L.) Osbeck cv. Tuyou were irrigated daily for 5 months with nutrient solution containing 0 (control), 0.2, 0.6 or 1.6 mM aluminum (Al) from AlCl3·6H2O. Shoot growth was more sensitive to Al toxicity than root growth, gas exchange, chlorophyll (Chl) concentration, polyphasic Chl a fluorescence (OJIP) induction and related parameters. Leaves of Al-treated plants showed d ...
... A series of crosses designed for introgression of mandarin (Citrus reticulata Blanco) and pummelo (C. maxima Merr.) germplasm, to develop an alternative rootstock to sour orange (C. aurantium L.), were carried out. It is necessary to identify those hybrids that yield nucellar seedlings for rootstock propagation. Rootstocks can be developed through traditional plant breeding methods; however, the a ...
... Huanglongbing (HLB, yellow shoot disease, ex. citrus greening disease), caused by Candidatus Liberibacter spp., is highly destructive to citrus production in Asia, Africa, and South America. Although primarily affecting sweet orange and mandarin, HLB has long been observed in pummelo in Guangdong Province, People's Republic of China; however, the disease in pummelo has received little research att ...
... Grapefruit growers in the tropics require information about existing and new citrus cultivars with high productivity potential. The objective of this study was to determine the growth, yield, and fruit quality performance of seven pigmented and four white grapefruit cultivars under the dry tropic conditions of Colima, Mexico. The trees were budded on sour orange (Citrus aurantium L.) rootstock and ...
ovules; seedless varieties; total soluble solids; cell walls; juice quality; cross pollination; fruit set; cell division; crop yield; titratable acidity; Citrus maxima; seed abortion; endosperm; pollen; irradiation; fruit juices; pummelos; X-radiation
Abstract:
... Cross-pollination was performed with soft-X-irradiated hyuga-natsu pollens (1000Gy) for 'Tosa-Buntan' pummelo (Citrus grandis (L.) Osbeck). This resulted in the transformation of large and complete seeds into small and empty ones (practically seedless). Although fruit set, fruit retention, total soluble solids content (TSS) and titratable acidity of the juice were not affected, decrement in the fr ...
... A farmers' participatory survey was carried out in the mid-hill and plain (Terai) regions of Nepal to assess the diversity, consumers' preference for fruit quality and potential for selection of elite pomelo clones. A wide variation of morphological characters of tree, fruit, leaf and seed was identified from information gathered and from laboratory analysis. Multivariate analysis of the data prod ...
Citrus maxima; Poncirus trifoliata; alleles; amplified fragment length polymorphism; apomixis; embryo sac; major genes; polyembryony; progeny; seedlings; seeds; segregation distortion
Abstract:
... Some citrus varieties express a form of apomixis termed nucellar embryony in which the adventive embryos develop from nucellus tissue surrounding the embryo sac. This trait results in many seeds containing multiple embryos (polyembryony). Inheritance of the frequency of polyembryony was studied in 88 progeny from a cross of Citrus maxima (monoembryonic) × Poncirus trifoliata (polyembryonic). The f ...
Citrus maxima; aluminum; boron; carbon dioxide; chlorophyll; electron transport chain; fluorescence; growth retardation; leaves; nutrient solutions; photochemistry; photosynthetic electron transport; pummelos; ribulose-bisphosphate carboxylase; root growth; toxicity
Abstract:
... BACKGROUND: Little information is available on the amelioration of boron (B) on aluminum (Al)-induced photosynthesis inhibition. Sour pummelo (Citrus grandis) seedlings were irrigated for 18 weeks with nutrient solution containing 4 B levels (2.5, 10, 25 and 50 μM H₃BO₃) × 2 Al levels (0 and 1.2 mM AlCl₃·6H₂O). The objectives of this study were to determine how B alleviates Al-induced growth inhib ...
glycosidases; fermentation; citrus fruits; naringinase; glycemic effect; plant extracts; enzymatic treatment; Citrus maxima; antioxidant activity; glycoflavonoids; flavanones; South Korea
Abstract:
... Dangyuja is a citrus fruit of Cheju Island in Korea, which is known to have a high content of flavanone glycosides, such as naringin and neohesperidin. Flavanone glycosides of Dangyuja (Citrus grandis Osbeck) extract were converted into their aglycones by naringinase and hesperidinase, and into their hydroxylated forms by Aspergillus saitoi. Dangyuja extract treated with A. saitoi had significantl ...
... Seedlings of Citrus grandis were fertilized every other days for 15 weeks with nutrient solution containing 0 (deficiency), 10μM (control) or 500μM (excess) H₃BO₃. CO₂ assimilation and chlorophyll (Chl) content decreased to a greater degree in B-deficient than in B-excess leaves, but photosynthetic enzyme activities were similarly decreased. Starch accumulated in B-deficient leaves, but not in B-e ...
... Seedlings of sour pummelo (Citrus grandis) were irrigated daily for 18 weeks with nutrient solution containing four phosphorus (P) levels (50, 100, 250 and 500 μM KH2PO4) and two aluminum (Al) levels [0 (−Al) and 1.2 mM AlCl3·6H2O (+Al)]. Both malate and citrate concentrations in +Al leaves decreased with increasing P supply, but their concentrations in −Al leaves did not change in response to P s ...
Citrus deliciosa; Citrus reshni; Citrus unshiu; mandarins; Citrus maxima; pummelos; phytoplasmal diseases; Candidatus Liberibacter asiaticus; pathotypes; genetic variation; geographical variation; pathogen identification; ribosomal DNA; DNA fingerprinting; internal transcribed spacers; genetic relationships; South East Asia
Abstract:
... The aim of this study was to investigate the genetic diversity and relationships among 'Candidatus Liberibacter asiaticus' isolates from different hosts and distinct geographical areas in Southeast Asia. Genetic diversity among 'Ca. Liberibacter asiaticus' was estimated by sequencing four well-characterized DNA fragments: the 16S ribosomal DNA (rDNA) and 16S/23S intergenic spacer regions; the oute ...
apoptosis; human cell lines; leukemia; hexane; fruit extracts; Citrus maxima; citrus fruits; cell proliferation; cytotoxicity; in vitro studies; enzyme activity
Abstract:
... The antiproliferative effect of an immature Citrus grandis Osbeck fruit extract was investigated using U937 human leukaemia cells. Maximum cytotoxicity was observed using the hexane fraction (HF) of the extract. Cell death was dose-dependent (IC ₅₀ =ca. 60μg/ml) and was characterised by chromatin condensation, apoptotic body formation, and DNA fragmentation. The induction of apoptosis was confirme ...
... BACKGROUND: New pomelo and mandarin scion varieties growing under subtropical arid conditions were investigated for total antioxidants in the juice. Four different rootstocks--sour orange (Citrus aurantium (L.); SO), Volkamer lemon (C. volkameriana (Ten & Pasq.)), SB812 (C. sunki (Hort. ex Tan.) x Poncirus trifoliate (L.)) and C. macrophylla (Wester)--were tested.RESULTS: Fruit juice was tested fo ...
flow resistance; mathematical models; shear strength; freeze drying; mass flow; concentrating; viscosity; activation energy; Citrus maxima; shear stress; total solids; temperature; fruit juices; pummelos; juice concentrates
Abstract:
... The rheological behaviour of freeze-dried-concentrated pummelo juice was modelled to investigate the effects of temperature and concentration on its fluid type and viscosity using a rotational viscometer at shear rates ranging from 1 to 400s⁻¹. The effect of concentration measured by its total soluble solids content resulted in the juice concentrates behaving towards shear thinning or pseudoplasti ...
... Limited data are available on the effects of phosphorus (P) and aluminum (Al) interactions on Citrus spp. growth and photosynthesis. Sour pummelo (Citrus grandis) seedlings were irrigated for 18 weeks with nutrient solution containing 50, 100, 250 and 500 μM KH₂PO₄x 0 and 1.2 mM AlCl₃· 6H₂O. Thereafter, P and Al in roots, stems and leaves, and leaf chlorophyll (Chl), CO₂ assimilation, ribulose-1,5 ...
... Citrus huanglongbing (HLB) or yellow shoot disease (i.e., greening disease) is highly destructive to citrus production worldwide. Understanding the etiology of HLB is critical for managing the disease. HLB is currently associated with infection by 'Candidatus Liberibacter spp.' around the world, including China. However, Koch's postulates have not been fulfilled. In addition, other plant pathogens ...
... The objective of this study was to identify the major volatiles and their relative concentrations in intact grapefruit, sweet orange, sour orange, mandarin, lemon, lime and pummelo blossoms. Volatiles from freshly picked blossoms were collected and concentrated using static headspace solid-phase microextraction and then separated and identified using GC-MS. Seventy volatiles were detected, 66 iden ...
Citrus maxima; crystallization; cultivars; high performance liquid chromatography; humans; medicinal properties; methanol; methylene chloride; naringin; nuclear magnetic resonance spectroscopy; pummelos
Abstract:
... An efficient and simple method was developed to isolate high-purity naringin, a bitter flavoured (to humans) flavanone glycoside with various pharmacological activities, from pomelo (Citrus grandis Osbeck) peel in high yield. The process, which involves a simple methanol extraction followed by crystallisation in water with the addition of 14-15% (v/v) dichloromethane, gave four times higher yield ...
citrus peels; acetic acid; fermented foods; alcoholic fermentation; essential oils; plant extracts; fruit pulp; Citrus maxima; fruit composition; flavonoids; antioxidant activity; fruit juices; ethanol; pummelos; phenolic compounds
Abstract:
... The antioxidant potentials of buntan pumelo (Citrus grandis Osbeck) and its fermented products were determined. The essential oil from peel had higher total phenolic (214mM) and flavonoid (134mg QE/g of dried material) contents than those of different solvent extracts from fruit pulp. In addition, DPPH free radical-scavenging activity and ferric-reducing antioxidant power values determined for the ...
... The study deals with antifungal, antiaflatoxigenic and antioxidant activity of Citrus maxima and Citrus sinensis essential oils (EOs) and their phytochemical composition. The EOs were obtained by hydrodistillation and their chemical profile was determined through GC and GC-MS analysis. Both the EOs and their 1:1 combination showed broad fungitoxic spectrum against different food contaminating moul ...
... The oils and extracts of the fruit peels of pomelo (Citrus maxima Merr. cultivar ‘khao–yai’) were obtained by cold-pressing (CP), vacuum steam distillation (VSD) and supercritical carbon dioxide extraction (SC-CO2) and the extract of the flowers was obtained by SC-CO2. The composition of the oils and extracts of the peel and flower were determined by GC and GC/MS. Fifty, 53 and 60 components of th ...
... The peel essential oils from four selected Tunisian Citrus species: sweet orange (Citrus sinensis Osbeck), mandarin (Citrus reticulata Blanco); sour orange (Citrus aurantium L.) and pummelo (Citrus grandis Osbeck), cultivated under the same pedoclimatic and cultural conditions have been analysed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The essential oils content ...
... Grapefruit is the fourth economically most important citrus fruit in the world. In this research Inter-Simple Sequence Repeat (ISSR) markers were used to distinguish twenty-nine grapefruit (Citrus paradisi Macf.), five pummelo (Citrus maxima (Burm.) Merr.) and one Citrus hassaku Hort. Ex Tanaka accessions. Twelve ISSR primers produced a total of 100 fragments and 62 of them were polymorphic. The n ...
... Background: Very little is known about the effects of manganese (Mn)-excess on citrus photosynthesis and antioxidant systems. Seedlings of sour pummelo (Citrus grandis) were irrigated for 17 weeks with nutrient solution containing 2 muM (control) or 500 muM (excess) MnSO4. The objective of this study were to understand the mechanisms by which Mn-excess leads to a decrease in CO2 assimilation and t ...
... Plant s-phase kinase-associated protein 1 (SKP1) genes have diverse functions in plant developmental and physiological activities. Herein, we described a novel SKP1 gene, designated as CgSKP1, from ‘Shatian' pummelo (Citrus grandis Osbeck). The cDNA sequence of CgSKP1 was 603 bp and contained an open reading frame of 477 bp. Genomic sequence of the CgSKP1 gene contained two exons and one intron. T ...
water uptake; magnetic resonance imaging; hybrids; image analysis; nuclear magnetic resonance spectroscopy; transpiration; fruit peels; Citrus maxima; horticulture; postharvest injuries; temperature; hot water treatment; Citrus paradisi; pummelos; imazalil
Abstract:
... As a non destructive analysis method, Nuclear Magnetic Resonance Imaging (MRI) may provide helpful information on water movement and physiological effects of transpiration in senescing tissues. In the past decade, the use of MRI has provided a better understanding of some physiological disorders in horticultural product (e.g., internal breakdown) and on quality changes during storage. Here we repo ...
... In order to predict the performance of growth, development and resistance of a citrus diploid cybrid plant between Citrus unshiu Marc. cv. Guoqing No. 1 (G1) and Hirado Buntan pummelo (Citrus grandis (L.) Osbeck) (HBP), the proteomes of leaves from this cybrid and its parents were investigated. First, the diploid cybrid plant (G1 + HBP) created with its nuclear and chloroplast genomes from HBP and ...
... The relative content of (2S)- and (2R)-naringin in the albedo of pummelo during maturation in the entire season was determined by normal-phase HPLC using Chiralpak IB, a polysaccharide-derived chiral stationary phase, and n-hexane/ethanol doped with 0.5% TFA as mobile phase. A sigmoid curve was obtained showing variation from 95.3% of (2S)-naringin in very immature fruits to 53% in mature fruit sa ...
Hevea brasiliensis; Mangifera indica; soil water balance; calibration; equations; sap flow; transpiration; tree physiology; Citrus maxima; data analysis; temperature; measurement; fruit trees; thermal analysis
Abstract:
... The transient thermal dissipation (TTD) method developed by Do and Rocheteau (2002b) is a close evolution of the original constant thermal dissipation (CTD) method of Granier (1985). The TTD method has the advantage of limiting the influence of passive natural temperature gradients and of yielding more stable zero-flux references at night. By analogy with the CTD method, the transient method was f ...
... In order to explore the potential use of Citrus grandis Osbeck in biomedical applications for improving human’s skin health, cotton knit fabric was dyed with the citrus extract under varied conditions including temperature, duration, pH, and dye concentration and evaluated for fastness and antimicrobial activity. The fabric was optimized for maximum dye uptake at a pH of 3, dyeing temperature of 6 ...
... This study aimed to investigate the skin irritancy with unripe Citrus grandis Osbeck extract and to determine the effect of cotton underclothes dyed with the citrus extract on the severity of atopic dermatitis by in-vivo methodology. The skin irritancy from the extract was assessed by Kawai’s method and the severity of atopic dermatitis was evaluated by measuring the quantitative index of SCORAD f ...
... Sour pummelo (Citrus grandis) seedlings were irrigated with nutrient solution containing four boron concentrations (i.e., 2.5, 10, 25 and 50 μM H3BO3) and two aluminum concentrations [i.e., 0 (-Al) and 1.2 mM AlCl3 · 6 H2O (+Al)]. It was found that B did not affect, but Al increased, the Al content in the roots. The Al and citrate contents in the -Al leaves either did not change or slightly increa ...
... ‘Zigui shatian’ pummelo (Citrus grandis Osbeck) is one nature mutant from ‘Shatian’ pummelo, which showed self-compatibility, because self-pollen tubes were not arrested in the style, moreover abnormal post-zygotic development in ovary caused seed abortion in the cultivar. Herein we constructed a cDNA library from flowers of ‘Zigui shatian’ pummelo and identified one RNase gene fragment. The full ...
... A study on the effect of starch-based edible coatings on shelf life and quality of minimally processed pummelo was conducted during storage at 4°C for 21 days. Starch-based coatings were developed from two types of native starches (rice and cassava) and mixed with two concentrations of pummelo juice (5 and 10% by volume) as anti-browning agents. It was found that the property of edible coating fro ...
Artocarpus heterophyllus; Citrus maxima; Citrus reticulata; Ipomoea batatas; Musa; case studies; cluster analysis; common names; foods; fuelwood; home gardens; households; medicine; plant anatomy; questionnaires; socioeconomic status; species diversity; subsistence farming; surveys; Vietnam
Abstract:
... Ethnobotanical survey was conducted in 101 randomly selected home gardens of Phong My commune, central Vietnam, situated in the buffer-zone of Natural Reserve. Data were collected through semi-structured questionnaires and direct observation. Sixty-seven species belonging to 35 families were identified to be used for various purposes. For each species the botanical and vernacular names, plant part ...
... ‘ Candidatus Liberibacter asiaticus’ was detected in Citrus decumana displaying symptoms of greening disease by direct polymerase chain reaction using universal primers specific to ribosomal protein gene of liberibacter. 100% Sequence identities were found with isolates of ‘Ca. L. asiaticus'—isolated from mandarins from Karnataka, India and more than 96% with other isolates of ‘Ca. L. asiaticus’ r ...
Citrus maxima; drug delivery systems; indomethacin; ions; pH; patients; pectins; pummelos; temperature
Abstract:
... The aim of this study was to extend the application of pectin extracted from pomelo peel to pharmaceutical suspensions. Particularly, the influence of pectin on the stability of indomethacin suspension was investigated. The use of cation, type and concentration of pectin, pH and temperature influenced the flocculating activity of pectin in suspension. The extracted pectin has comparable activity t ...
... Genetic diversity was evaluated by sequence related amplified polymorphism (SRAP) markers among 27 grapefruit (Citrus paradisi Macf.), 5 pummelo (Citrus maxima (Burm.) Merr.) accessions and 4 pummelo hybrids. Twenty-one SRAP primer combinations produced a total of 90 polymorphic fragments with an average of 4.3 per primer combinations. The mean polymorphism information content (PIC) was 0.34. The ...
Citrus maxima; acidity; clones; color; fruits; genetic improvement; genetic variation; genotype; heritability; juices; pummelos; raw fruit; ripening; seeds; sugars; surveys; total soluble solids; trees; India
Abstract:
... Survey for selection of elite clones of pummelo was initiated in 1999. Since then more than 700 trees were evaluated and 15 elite clones were identified as superior clones. Wide variability in yield (84-315 fruits per tree), fruit weight (570-2010 g), fruit shape, peel color, number of segments per fruit (12-16), number of seeds per fruit (67-182), total soluble solids (9-11.4°Brix), total sugar ( ...
... Malaysian pink and white pomelo (Citrusgrandis (L.) Osbeck) peels were extracted with dichloromethane (DCM). Using GC-FID/MS and gas chromatography-olfactometry (GC-O), 50 and 47 aroma-active compounds were identified in pink and white pomelo peel extracts, respectively. The potency of each odorant in both pomelo peel extracts was determined by aroma extraction dilution analysis (AEDA). On the bas ...
... Pummelo, a citrus fruit, is an underutilized fruit crop with a potential for commercialization in warm humid climates. Improvement of crops through bio-technological approaches mainly involves availability of efficient in vitro regeneration protocols. A study was conducted to develop a regeneration protocol for a local cultivar of pummelo under cultivation in Devanahalli, Bangalore, India, using c ...
... Ten Citrus (Linnaeus, 1753) species of North-East India have been karyo-morphologically analysed. All studied species had 2n=18 chromosomes without any evidence of numerical variation. All the chromosomes were found to be of metacentric and sub-metacentric in all the species; the morphology of the chromosomes showing size difference only. Symmetrical karyotype which does not have much difference i ...
... ‘Sour pummelo’ (Citrus grandis) and ‘Xuegan’ (C. sinensis) seedlings were irrigated for 18 weeks with nutrient solution containing 0 (−Al) and 1.2mM AlCl₃·6H₂O (+Al)×0, 50 and 200μM KH₂PO₄. C. sinensis was more tolerant to aluminum (Al) than C. grandis. Phosphorus (P) alleviated the toxic effects of Al on seedlings. Under Al stress, P increased root Al, but decreased shoot Al; C. sinensis accumula ...
... The aim of this work was to provide a set of mitochondrial markers to reveal polymorphism and to study the maternal phylogeny in citrus. We first used 44 universal markers previously described in the literature: nine of these markers produced amplification products but only one revealed polymorphism in citrus. We then designed six conserved pairs of primers using the complete mitochondrial DNA seq ...
... The Asian form of huanglongbing (HLB) is caused by ‘Candidatus Liberibacter asiaticus (Las),’ a phloem-limited bacterium transmitted by the Asian citrus psyllid, Diaphorina citri Kuwayama. Nutrient management, together with other cultural practices such as pruning and irrigation, for mitigation of the disease has been practiced in China for many years. Our literature review, field survey, and inte ...
... ‘Shatian' pummelo (Citrus grandis Osbeck), one of the main citrus cultivars in China, is self-incompatible, and its pollen tubes are believed to be arrested in style after self-pollination.We have characterized one ‘Shatian' pummelo mutant, named ‘Zigui shatian' pummelo. The mutant pummelo had identical DNA ploidy level, morphology (leaf shape, stoma size and density, pollen shape and size) and de ...
Citrus maxima; Prays; control components; dispensers; gas chromatography; insecticides; mass spectrometry; mating disruption; monitoring; orchards; pests; pummelos; sex pheromones; traps; Vietnam
Abstract:
... The citrus pock caterpillar, Prays endocarpa (Yponomeutidae; Praydinae), is a pest of pomelo (Citrus grandis L.) in Vietnam. Gas chromatography-mass spectrometry analyses of pheromone gland extracts from female moths identified three monoenyl compounds, (Z)-7-tetradecenal (Z7-14:Ald), (Z)-7-tetradecenyl acetate (tentatively identified, Z7-14:OAc), and (Z)-7-tetradecen-1-ol (Z7-14:OH), in a ratio o ...
Citrus maxima; Cydonia oblonga; DNA; Erwinia amylovora; apples; biological control; climate; disease control; genetic markers; leaves; pathogen characterization; pathogens; pummelos; quinces; random amplified polymorphic DNA technique; trees; Romania
Abstract:
... Fire blight (Erwinia amylovora) was first observed on apple trees in 1992 in Pitesti (Maracineni) and Braila counties of southeast Romania. Due to several factors, including climate, underestimated loss and lack of efficient control measures, the disease spread to 42 counties in Romania by the year 2000. From 2004 to 2006, 34,785.3 ha (46% of cultivated areas) were inspected for fire blight occurr ...