U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject term Cryphonectria parasitica Remove constraint Subject term: Cryphonectria parasitica
Start Over

Search

395  Search Results

« Previous | 1 - 100 of 395 |
Number of results to display per page

Search Results

1. A 971-bp insertion in the rns gene is associated with mitochondrial hypovirulence in a strain of Cryphonectria parasitica isolated from nature

Author:
Dipnath Baidyaroy; Georg Hausner; Mohamed Hafez; Francois Michel; Dennis W. Fulbright; Helmut Bertrand
Source:
Fungal genetics and biology 2011 v.48 no.8 pp. 775-783
ISSN:
1087-1845
Subject:
sequence analysis; protein synthesis; ribosomal RNA; phenotype; open reading frames; mitochondrial DNA; reverse transcriptase polymerase chain reaction; ribosomes; cyanide-resistant respiration; viruses; exons; double-stranded RNA; Cryphonectria parasitica; virulence; introns; virulent strains; fungi
Abstract:
... In the chestnut-blight fungus Cryphonectria parasitica, cytoplasmically transmissible hypovirulence phenotypes frequently are elicited by double-stranded RNA (dsRNA) virus infections. However, some strains manifest cytoplasmically transmissible hypovirulence traits without containing any mycovirus. In this study, we describe an altered form of mtDNA that is associated with hypovirulence and senesc ...
DOI:
10.1016/j.fgb.2011.05.006
PubMed:
21601643

2. A Mutant of the Bck1 Homolog from Cryphonectria parasitica Resulted in Sectorization with an Impaired Pathogenicity

Author:
Kim Jung-Mi; Lee Joong-Gi; Yun Suk-Hyun; So Kum-Kang; Ko Yo-Han; Kim Young Ho; Park Seung-Moon; Kim Dae-Hyuk
Source:
Molecular plant-microbe interactions 2016 v.29 no.4 pp. 268-276
ISSN:
0894-0282
Subject:
Cryphonectria parasitica; Saccharomyces cerevisiae; cell walls; conidiation; fungi; growth retardation; hypersensitivity; hyphae; inheritance (genetics); mitogen-activated protein kinase kinase kinase; mutants; mutation; mycelium; phenotype; pigmentation; progeny; signal transduction; virulence
Abstract:
... CpBck1, an ortholog of the cell-wall integrity mitogen-activated protein kinase kinase kinase of Saccharomyces cerevisiae, was cloned and characterized from the chestnut blight fungus Cryphonectria parasitica. The CpBck1-null mutant displayed cell wall integrity–related phenotypic changes such as abnormal cell morphology and wall formation and hypersensitivity to cell wall-disrupting agents. In ad ...
DOI:
10.1094/MPMI-08-15-0185-R

3. A Tannic Acid-Inducible and Hypoviral-Regulated Laccase3 Contributes to the Virulence of the Chestnut Blight Fungus Cryphonectria parasitica

Author:
Chung, Hea-Jong; Kwon, Bo-Ra; Kim, Jung-Mi; Park, Seung-Moon; Park, Jong-Kun; Cha, Byeong-Jin; Yang, Moon-Sik; Kim, Dae-Hyuk
Source:
Molecular plant-microbe interactions 2008 v.21 no.12 pp. 1582-1590
ISSN:
0894-0282
Subject:
Cryphonectria parasitica; plant pathogenic fungi; fungal diseases of plants; Hypovirus; tannins; laccase; gene expression regulation; virulence; bark; tree diseases; enzyme activation; ferulic acid; catechol; pH; transcription (genetics); gene expression; enzyme activity; fungal anatomy; sporulation; pigmentation; lesions (plant)
Abstract:
... A new laccase gene (lac3) from the chestnut blight fungus Cryphonectria parasitica was induced by the presence of tannic acid, which is abundant in the bark of chestnut trees and is assumed to be one of the major barriers against pathogen infection. However, other commonly known laccase inducers, including ferulic acid, 2,5-xylidine, catechol, and pH, did not induce lac3 transcription. Moreover, t ...
DOI:
10.1094/MPMI-21-12-1582
PubMed:
18986254

4. A circular mitochondrial plasmid incites hypovirulence in some strains of Cryphonectria parasitica

Author:
Monteiro-Vitorello, C.B.; Baidyaroy, D.; Bell, J.A.; Hausner, G.; Fulbright, D.W.; Betrand, H.
Source:
Current genetics 2000 v.37 no.4 pp. 242-256
ISSN:
0172-8083
Subject:
strains; messenger RNA; restriction mapping; genetic code; mitochondrial DNA; open reading frames; geographical variation; hyphae; plasmids; gene expression; transcription (genetics); Malus domestica; DNA-directed DNA polymerase; cytoplasmic inheritance; Cryphonectria parasitica; virulence; United States; Ontario
DOI:
10.1007/s002940050526
PubMed:
10803886

5. A comparison of times series approaches for dendroecological reconstructions of past canopy disturbance events

Author:
Daniel L. Druckenbrod; Neil Pederson; James Rentch; Edward R. Cook
Source:
Forest ecology and management 2013 v.302 pp. 23-33
ISSN:
0378-1127
Subject:
Cryphonectria parasitica; Quercus montana; Quercus rubra; Tsuga canadensis; biomass; canopy; carbon; climate; forest management; forest thinning; forests; growth rings; logging; time series analysis; tree growth; New York; Pennsylvania; West Virginia
Abstract:
... Time series analysis can identify outliers in tree-ring widths that may not only indicate past disturbances, but may also estimate the subsequent effects of these disturbances on tree growth. Finding a way to isolate these disturbance signals from tree-ring time series could have broad applications in forest ecology and management. Time series outliers may be expressed as pulse, step, or trend int ...
DOI:
10.1016/j.foreco.2013.03.040

6. A gene encoding phosphatidyl inositol-specific phospholipase C from Cryphonectria parasitica modulates the lac1 expression

Author:
Chung, H.J.; Kim, M.J.; Lim, J.Y.; Park, S.M.; Cha, B.J.; Kim, Y.H.; Yang, M.S.; Kim, D.H.
Source:
Fungal genetics and biology 2006 v.43 no.5 pp. 326-336
ISSN:
1087-1845
Subject:
Cryphonectria parasitica; plant pathogenic fungi; genes; phospholipase C; gene expression regulation; laccase; nucleotide sequences; amino acid sequences; mutants; virulence; blight; Castanea dentata
DOI:
10.1016/j.fgb.2005.12.009
PubMed:
16540355

7. A host dicer is required for defective viral RNA production and recombinant virus vector RNA instability for a positive sense RNA virus

Author:
Zhang, Xuemin; Nuss, Donald L.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2008 v.105 no.43 pp. 16749-16754
ISSN:
0027-8424
Subject:
genes; mutants; small interfering RNA; Cryphonectria parasitica; genetic vectors; Cryphonectria hypovirus 1; genetic recombination
Abstract:
... Defective interfering (DI) RNAs, helper virus-dependent deletion mutant RNAs derived from the parental viral genomic RNA during replication, have been described for most RNA virus taxonomic groups. We now report that DI RNA production in the chestnut blight fungus, Cryphonectria parasitica, persistently infected by virulence-attenuating positive sense RNA hypoviruses, depends on one of two host di ...
DOI:
10.1073/pnas.0807225105
PubMed:
18922782
PubMed Central:
PMC2567904

8. A hypovirulent isolate of Cryphonectria parasitica with multiple, genetically unique dsRNA segments

Author:
Enebak, S.A.; Hillman, B.I.; MacDonald, W.L.
Source:
Molecular plant-microbe interactions 1994 v.7 no.5 pp. 590-595
ISSN:
0894-0282
Subject:
blight; apples; sporulation; Reoviridae; fungal diseases of plants; viruses; double-stranded RNA; Castanea sativa; Cryphonectria parasitica; virulence
DOI:
10.1094/MPMI-7-0590

9. A long open reading frame in the mitochondrial LSU rRNA group-I intron of Cryphonectria parasitica encodes a putative S5 ribosomal protein fused to a maturase

Author:
Hausner, G.; Monteiro-Vitorello, C.B.; Searles, D.B.; Maland, M.; Fulbright, D.W.; Bertrand, H.
Source:
Current genetics 1999 v.35 no.2 pp. 109-117
ISSN:
0172-8083
Subject:
ribosomal RNA; open reading frames; mitochondrial DNA; ribosomal DNA; ribosomes; binding proteins; amino acid sequences; nucleases; Cryphonectria parasitica; introns
DOI:
10.1007/s002940050439
PubMed:
10079329

10. A metabolomic study of vegetative incompatibility in Cryphonectria parasitica

Author:
Thomas E. Witte; Sam Shields; Graham W. Heberlig; Mike G. Darnowski; Anatoly Belov; Amanda Sproule; Christopher N. Boddy; David P. Overy; Myron L. Smith
Source:
Fungal genetics and biology 2021 v.157 pp. 103633
ISSN:
1087-1845
Subject:
Castanea; Cryphonectria parasitica; Hypoviridae; biological control; biosynthesis; conspecificity; culture media; heterokaryon; hyphae; lysophosphatidylcholine; mass spectrometry; metabolome; metabolomics; microbial genetics; mycelium; nuclear magnetic resonance spectroscopy; secondary metabolites; transcriptomics; virulent strains
Abstract:
... Vegetative incompatibility (VI) is a form of non-self allorecognition in filamentous fungi that restricts conspecific hyphal fusion and the formation of heterokaryons. In the chestnut pathogenic fungus, Cryphonectria parasitica, VI is controlled by six vic loci and has been of particular interest because it impedes the spread of hypoviruses and thus biocontrol strategies. We use nuclear magnetic r ...
DOI:
10.1016/j.fgb.2021.103633

11. A multiplexed genotyping assay to determine vegetative incompatibility and mating type in Cryphonectria parasitica

Author:
C. Cornejo; B. Šever; Q. Kupper; S. Prospero; D. Rigling
Source:
European journal of plant pathology 2019 v.155 no.1 pp. 81-91
ISSN:
0929-1873
Subject:
Cryphonectria naterciae; Cryphonectria parasitica; DNA; biological control; detection limit; fluorescence; genotyping; loci; mycoviruses; polymerase chain reaction; virulence; virulent strains
Abstract:
... The highly destructive chestnut blight disease can be successfully controlled by infecting the virulent strain of C. parasitica with the hyperparasitic mycovirus CHV-1. The artificial application of the virus-induced hypovirulence, however, requires that the vegetative compatibility (vc) diversity in the target C. parasitica population be determined beforehand. Conventional vc type determination b ...
DOI:
10.1007/s10658-019-01751-w

12. A new extracellular laccase of Cryphonectria parasitica is revealed by deletion of Lac1

Author:
Kim, D.H.; Rigling, D.; Zhang, L.; Van Alfen, N.K.
Source:
Molecular plant-microbe interactions 1995 v.8 no.2 pp. 259-266
ISSN:
0894-0282
Subject:
Cryphonectria parasitica; structural genes; laccase; virulence; pigmentation; sporulation; conidia; spore germination; sexual reproduction; enzyme activity; blight; Castanea dentata; phenotype
Abstract:
... The biological function of the extracellular laccase (Laccase 1) of Cryphonectria parasitica was investigated by deletion of Lac1 using recombinant DNA techniques. Lac1 is one of the genes of this fungus which is down-regulated by the hypovirulence-causing dsRNA virus, CHV1. This virus causes a variety of symptoms of the fungus, including hypovirulence, reduced sporulation, and reduced pigmentatio ...
DOI:
10.1094/MPMI-8-0259

13. A novel betapartitivirus RnPV6 from Rosellinia necatrix tolerates host RNA silencing but is interfered by its defective RNAs

Author:
Sotaro Chiba; Yu-Hsin Lin; Hideki Kondo; Satoko Kanematsu; Nobuhiro Suzuki
Source:
Virus research 2016 v.219 pp. 62-72
ISSN:
0168-1702
Subject:
coat proteins; RNA interference; phenotype; pigmentation; Rosellinia necatrix; virion; Partitiviridae; genome; models; root rot; transfection; pathogenicity; plants (botany); plant pathogenic fungi; RNA-directed RNA polymerase; viruses; double-stranded RNA; protists; Cryphonectria parasitica
Abstract:
... The family Partitiviridae comprises of five genera with bi-segmented dsRNA genomes that accommodate members infecting plants, fungi or protists. All partitiviruses with only a few exceptions cause asymptomatic infections. We report the characterization of a novel betapartitivirus termed Rosellinia necatrix partitivirus 6 (RnPV6) from a field isolate of a plant pathogenic fungus, white root rot fun ...
DOI:
10.1016/j.virusres.2015.10.017

14. A novel mycovirus isolated from the plant-pathogenic fungus Botryosphaeria dothidea

Author:
Hong Liu; Hui Wang; Qian Zhou
Source:
Archives of virology 2021 v.166 no.4 pp. 1267-1272
ISSN:
0304-8608
Subject:
Botryosphaeria dothidea; Cryphonectria parasitica; Partitiviridae; RNA-directed RNA polymerase; genome; genomics; mycoviruses; phylogeny; plant pathogenic fungi; virology
Abstract:
... A novel virus, Botryosphaeria dothidea bipartite mycovirus 1 (BdBMV1), was isolated from the plant-pathogenic fungus Botryosphaeria dothidea strain HNDT1, and the complete nucleotide sequence of its genome was determined. BdBMV1 consists of two genomic segments. The first segment is 1,976 bp in length and contains a single open reading frame (ORF) encoding the RNA-dependent RNA polymerase (RdRp) ( ...
DOI:
10.1007/s00705-021-04983-6

15. A novel victorivirus from the phytopathogenic fungus Neofusicoccum parvum

Author:
Haris Ahmed Khan; Yukiyo Sato; Hideki Kondo; Atif Jamal; Muhammad Faraz Bhatti; Nobuhiro Suzuki
Source:
Archives of virology 2022 v.167 no.3 pp. 923-929
ISSN:
0304-8608
Subject:
Cryphonectria parasitica; Neofusicoccum parvum; RNA-directed RNA polymerase; Victorivirus; agarose; amino acid sequences; coat proteins; gels; mycoviruses; plant pathogenic fungi; sequence analysis; virology
Abstract:
... Neofusicoccum parvum is an important plant-pathogenic ascomycetous fungus that causes trunk diseases in a variety of plants. A limited number of reports on mycoviruses from this fungus are available. Here, we report the characterization of a novel victorivirus, Neofusicoccum parvum victorivirus 3 (NpVV3). An agarose gel dsRNA profile of a Pakistani strain of N. parvum, NFN, showed a band of ~5 kbp ...
DOI:
10.1007/s00705-021-05304-7

16. A real‐time PCR assay for improved rapid, specific detection of Cryphonectria parasitica

Author:
S. Rubio; A. Barnes; K. Webb; J. Hodgetts
Source:
Annals of applied biology 2017 v.171 no.1 pp. 52-61
ISSN:
0003-4746
Subject:
Castanea; Cryphonectria parasitica; DNA; analytical specificity; detection limit; diagnostic sensitivity; fungi; hosts; pathogens; plant protection; quantitative polymerase chain reaction; trees; virulence; Europe
Abstract:
... Cryphonectria parasitica, an ascomycete fungus, is the causal agent of chestnut blight. This highly destructive disease of chestnut trees causes significant losses, and is therefore a regulated pathogen in Europe. Existing methods for the detection of C. parasitica include morphological identification following culturing, or PCR; however, these are time‐consuming resulting in delays to diagnosis. ...
DOI:
10.1111/aab.12354

17. A single Argonaute gene is required for induction of RNA silencing antiviral defense and promotes viral RNA recombination

Author:
Sun, Qihong; Choi, Gil H.; Nuss, Donald L.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2009 v.106 no.42 pp. 17927-17932
ISSN:
0027-8424
Subject:
gene silencing; gene expression; genes; RNA; Cryphonectria parasitica; microbial genetics; Cryphonectria hypovirus 1; genetic recombination
Abstract:
... Dicer gene dcl2, required for the RNA silencing antiviral defense response in the chestnut blight fungus Cryphonectria parasitica, is inducible upon mycovirus infection and promotes viral RNA recombination. We now report that the antiviral defense response requires only one of the four C. parasitica Argonaute-like protein genes, agl2. The agl2 gene is required for the virus-induced increase in dcl ...
DOI:
10.1073/pnas.0907552106
PubMed:
19822766
PubMed Central:
PMC2760488

18. A small mitochondrial double-stranded (ds) RNA element associated with a hypovirulent strain of the chestnut blight fungus and ancestrally related to yeast cytoplasmic T and W dsRNAs

Author:
Polashock, J.J.; Hillman, B.I.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1994 v.91 no.18 pp. 8680-8684
ISSN:
0027-8424
Subject:
apples; sporulation; complementary DNA; restriction mapping; Saccharomyces cerevisiae; mitochondria; nucleotide sequences; double-stranded RNA; Cryphonectria parasitica; virulence
Abstract:
... A small double-stranded (ds) RNA element was isolated from a moderately hypovirulent strain of the chestnut blight fungus Cryphonectria parasitica (Murr.) Barr. from eastern New Jersey. Virulence was somewhat lower in the dsRNA-containing strain than in a virulent dsRNA-free control strain, but colony morphology and sporulation levels were comparable. A library of cDNA clones was constructed, and ...
DOI:
10.1073/pnas.91.18.8680
PubMed:
7521532
PubMed Central:
PMC44670

19. A split luciferase complementation assay for studying in vivo protein–protein interactions in filamentous ascomycetes

Author:
Hee-Kyoung Kim; Eun Ji Cho; Seong mi Jo; Bo Reum Sung; Seunghoon Lee; Sung-Hwan Yun
Source:
Current genetics 2012 v.58 no.3 pp. 179-189
ISSN:
0172-8083
Subject:
Fusarium graminearum; hygromycin B; transcription factors; luminescence; luciferase; yeasts; Bipolaris maydis; F-box proteins; genes; fungal proteins; Cryphonectria parasitica
Abstract:
... Protein–protein interactions play important roles in controlling many cellular events. To date, several techniques have been developed for detection of protein–protein interactions in living cells, among which split luciferase complementation has been applied in animal and plant cells. Here, we examined whether the split luciferase assay could be used in filamentous ascomycetes, such as Gibberella ...
DOI:
10.1007/s00294-012-0375-5
PubMed:
22531843

20. A structural homologue of the plant receptor D14 mediates responses to strigolactones in the fungal phytopathogen Cryphonectria parasitica

Author:
Valentina Fiorilli; Marco Forgia; Alexandre de Saint Germain; Giulia D’Arrigo; David Cornu; Philippe Le Bris; Salim Al‐Babili; Francesca Cardinale; Cristina Prandi; Francesca Spyrakis; François‐Didier Boyer; Massimo Turina; Luisa Lanfranco
Source:
The new phytologist 2022 v.234 no.3 pp. 1003-1017
ISSN:
0028-646X
Subject:
Arabidopsis thaliana; Cryphonectria parasitica; enzyme activity; gene deletion; gene silencing; homologous recombination; loss-of-function mutation; models; mutants; phenotype; plant pathogens; prediction; strigolactones; vesicular arbuscular mycorrhizae
Abstract:
... Strigolactones (SLs) are plant hormones and important signalling molecules required to promote arbuscular mycorrhizal (AM) symbiosis. While in plants an α/β‐hydrolase, DWARF14 (D14), was shown to act as a receptor that binds and cleaves SLs, the fungal receptor for SLs is unknown. Since AM fungi are currently not genetically tractable, in this study, we used the fungal pathogen Cryphonectria paras ...
DOI:
10.1111/nph.18013

21. A transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica)

Author:
T. L. Kubisiak; C. D. Nelson; M. E. Staton; T. Zhebentyayeva; C. Smith; B. A. Olukolu; G.-C. Fang; F. V. Hebard; S. Anagnostakis; N. Wheeler; P. H. Sisco; A. G. Abbott; R. R. Sederoff
Source:
Tree genetics & genomes 2013 v.9 no.2 pp. 557-571
ISSN:
1614-2942
Subject:
genome assembly; Prunus persica; Cryphonectria parasitica; genetic markers; quantitative trait loci; blight; linkage groups; parents; powdery mildew; disease resistance; pathogens; introgression; Castanea mollissima; models; progeny; single nucleotide polymorphism; Castanea dentata; loci; peaches; expressed sequence tags; fungi
Abstract:
... The Chinese chestnut (Castanea mollissima) carries resistance to Cryphonectria parasitica, the fungal pathogen inciting chestnut blight. The pathogen, introduced from Asia, devastated the American chestnut (Castanea dentata) throughout its native range early in the twentieth century. A highly informative genetic map of Chinese chestnut was constructed to extend genomic studies in the Fagaceae and ...
DOI:
10.1007/s11295-012-0579-3

22. Aboveground carbon biomass of plantation-grown American chestnut (Castanea dentata) in absence of blight

Author:
Jacobs, Douglass F.; Selig, Marcus F.; Severeid, Larry R.
Source:
Forest ecology and management 2009 v.258 no.3 pp. 288-294
ISSN:
0378-1127
Subject:
Castanea dentata; hybrids; carbon sequestration; forest plantations; Juglans nigra; Quercus rubra; dry matter accumulation; tree growth; trees; disease resistance; Cryphonectria parasitica; blight; Wisconsin
Abstract:
... Forest management activities may help reduce global net CO₂ concentrations by capturing and storing atmospheric CO₂. Research related to carbon sequestration potential of plantations in North America has focused predominantly on conifers, with relatively little emphasis thus far on temperate deciduous forest tree species. American chestnut (Castanea dentata (Marsh.) Borkh.), a former dominant tree ...
DOI:
10.1016/j.foreco.2009.04.014

23. Agro-Industrial Wastes: A Substrate for Multi-Enzymes Production by Cryphonectria parasitica

Author:
Salvatore Savino; Daniela Bulgari; Eugenio Monti; Emanuela Gobbi
Source:
Fermentation 2021 v.7 no.4 pp. -
ISSN:
2311-5637
Subject:
Cryphonectria parasitica; carboxylesterase; enzyme activity; feeds; humans; laccase; polyacrylamide gel electrophoresis; proteinases; solid state fermentation; wheat bran
Abstract:
... This study aims to produce a mix of enzymes through Solid State Fermentation (SSF) of raw materials. Four different, easily available, agro-industrial wastes were evaluated as SSF substrates for enzymes production by Cryphonectria parasitica (Murr.) Barr. environmental strains named CpA, CpB2, CpC4, and CpC7. Among the tested wastes, organic wheat bran for human use and wheat bran for animal feed ...
DOI:
10.3390/fermentation7040279

24. Agrobacterium-mediated transformation of European chestnut somatic embryos with a Castanea sativa (Mill.) endochitinase gene

Author:
E. Corredoira; M. C. San José; A. M. Vieitez; I. Allona; C. Aragoncillo; A. Ballester
Source:
New forests 2016 v.47 no.5 pp. 669-684
ISSN:
0169-4286
Subject:
Castanea sativa; Cryphonectria parasitica; Southern blotting; cell wall components; chitinase; cryopreservation; cytotoxicity; disease resistance; embryogenesis; explants; fluorescence; fungi; gene overexpression; germination; green fluorescent protein; leaves; phenotype; polymerase chain reaction; shoots; silver thiosulfate; somatic embryos; transgenes; transgenic plants; vitrification; Europe; North America
Abstract:
... Chestnut blight, caused by Cryphonectria parasitica, is a severe disease that has devastated chestnut stands in North America and Europe. Genes encoding hydrolytic enzymes such as chitinases, which can degrade fungal cell wall components, are attractive candidates for improving disease resistance. This report describes a reliable and efficient protocol for the Agrobacterium-mediated transformation ...
DOI:
10.1007/s11056-016-9537-5

25. American Chestnut Growth and Survival Five Years after Planting in Two Silvicultural Treatments in the Southern Appalachians, USA

Author:
Stacy Clark; Henry McNab; David Loftis; Stanley Zarnoch
Source:
Forests 2012 v.3 no.4 pp. 1017-1033
ISSN:
1999-4907
Subject:
Castanea dentata; Cryphonectria parasitica; blight; forests; growing season; harvesting; planting; seedlings; shelterwood systems; trees; Appalachian region; United States
Abstract:
... The ability to restore American chestnut (Castanea dentata) through the planting of blight-resistant (Cryphonectria parasitica) trees is currently being tested. Forest-based research on the species’ silvicultural requirements and chestnut blight development are lacking. Pure American chestnut seedlings were planted in a two-age shelterwood forest with low residual basal area and in a midstory-remo ...
DOI:
10.3390/f3041017

26. American chestnut (Castanea dentata) to northern red oak (Quercus rubra): forest dynamics of an old-growth forest in the Blue Ridge Mountains, USA

Author:
Saskia L. van de Gevel; Justin L. Hart; Mark D. Spond; Philip B. White; Megan N. Sutton; Henri D. Grissino-Mayer
Source:
Botany 2012 v.90 no.12 pp. 1263-1276
ISSN:
1916-2804
Subject:
Acer saccharum subsp. saccharum; Castanea dentata; Cryphonectria parasitica; Quercus rubra; age structure; canopy; climate; fire scars; fires; forest inventory; forest management; grazing; hardwood; ice; livestock; logging; old-growth forests; species diversity; storms; temperature; trees; wind; Blue Ridge Mountain region; North Carolina
Abstract:
... We analyzed tree species composition and age structure in a rare, old-growth Quercus rubra L. (northern red oak) forest at Bluff Mountain Preserve, North Carolina, to assess potential changes associated with Cryphonectria parasitica (Murrill) M.E. Barr (chestnut blight), selective logging, livestock grazing, ice storms, wind events, and fire history. We established forest inventory plots to determ ...
DOI:
10.1139/b2012-100

27. American chestnut restoration

Author:
K. Collins; S. Fitzsimmons; T. Saielli; J. Westbrook
Source:
Acta horticulturae 2017 no.1174 pp. 215-218
ISSN:
0567-7572
Subject:
Castanea crenata; Castanea dentata; Castanea mollissima; Cryphonectria parasitica; backcrossing; blight; breeding programs; disease resistance; genetic variation; hybrids; interspecific hybridization; pathogens; tree breeding
Abstract:
... Breeding American chestnuts for resistance to chestnut blight has been on-going in some capacity since the early twentieth century. Early efforts focused on hybridizing American chestnut with Chinese chestnut (Castanea mollissima) and Japanese chestnut (Castanea crenata), in order to capture the pathogen resistance of these Asian species. The breeding program of The American Chestnut Foundation wa ...
DOI:
10.17660/ActaHortic.2017.1174.44

28. American chestnut soil carbon and nitrogen dynamics: Implications for ecosystem response following restoration

Author:
Geoffrey W. Schwaner; Charlene N. Kelly
Source:
Pedobiologia 2019 v.75 pp. 24-33
ISSN:
0031-4056
Subject:
Castanea dentata; Cryphonectria parasitica; Prunus serotina; Quercus rubra; biogeochemistry; cherries; deciduous forests; dissolved organic carbon; ecosystems; enzyme activity; extracellular enzymes; fungi; isotope labeling; leachates; leaching; nitrogen; soil; soil carbon; soil respiration; stable isotopes; trees; North America
Abstract:
... American chestnut (Castenea dentata), once dominant throughout the eastern deciduous forest of North America, was extirpated from its native range by chestnut blight fungus. Through development of blight-resistant trees, the reintroduction of chestnut is likely, though little is known about the biogeochemistry of forests influenced by chestnut. We performed a one-year laboratory incubation experim ...
DOI:
10.1016/j.pedobi.2019.05.003
CHORUS:
10.1016/j.pedobi.2019.05.003

29. American chestnut sprout dynamics

Author:
Stevens, D.L.; Soltau, K.; Davelos Baines, A.; Jarosz, A.M.
Source:
Acta horticulturae 2014 v. no.1019 pp. 223-227
ISSN:
0567-7572
Subject:
Castanea dentata; Cryphonectria parasitica; chestnuts; death; fungi; root crown; stem elongation; stems; tree and stand measurements; trees; Michigan; Wisconsin
Abstract:
... American chestnut (Castanea dentata) populations have been decimated over the last 100 years by the chestnut blight fungus Cryphonectria parasitica. The species can produce prolific numbers of root collar sprouts and often, after the death of the main stem, a number of root sprouts result. The effects of three treatments were compared to assess sprout growth and sprout number among seven American ...

30. American chestnut survival in understory mesic sites following the chestnut blight pandemic

Author:
Griffin, G.J.
Source:
Canadian journal of botany 1992 v.70 no.10 pp. 1950-1956
ISSN:
0008-4026
Subject:
Castanea dentata; blight; forest trees; Acer rubrum; soil water regimes; stand basal area; Cryphonectria parasitica; mortality; understory; Virginia; Tennessee
DOI:
10.1139/b92-243

31. Amino acid sequence of endothiapepsin. Complete primary structure of the aspartic protease from Endothia parasitica

Author:
Barkholt, V.
Source:
European journal of biochemistry 1987 v.167 no.2 pp. 327-338
ISSN:
0014-2956
Subject:
amino acids; Cryphonectria parasitica; pepsin
DOI:
10.1111/j.1432-1033.1987.tb13340.x
PubMed:
3305016

32. Analysis of Environment-Marker Associations in American Chestnut

Author:
Markus Müller; C. Dana Nelson; Oliver Gailing
Source:
Forests 2018 v.9 no.11 pp. -
ISSN:
1999-4907
Subject:
Castanea dentata; Cryphonectria parasitica; breeding; environmental factors; expressed sequence tags; fungi; genetic engineering; genetic variation; genomics; genotyping; geographical variation; loci; microsatellite repeats; nursery stock; provenance; trees; Appalachian region; North America
Abstract:
... American chestnut (Castanea dentata Borkh.) was a dominant tree species in its native range in eastern North America until the accidentally introduced fungus Cryphonectria parasitica (Murr.) Barr, that causes chestnut blight, led to a collapse of the species. Different approaches (e.g., genetic engineering or conventional breeding) are being used to fight against chestnut blight and to reintroduce ...
DOI:
10.3390/f9110695

33. Analysis of altered G-protein subunit accumulation in Cryphonectria parasitica reveals a third Gα homologue

Author:
Todd B. Parsley; Gert C. Segers; Donald L. Nuss; Angus L. Dawe
Source:
Current genetics 2003 v.43 no.1 pp. 24-33
ISSN:
0172-8083
Subject:
Cryphonectria parasitica; G-proteins; antiserum; buffers; eukaryotic cells; fungi; membrane proteins; mutants; pathogenicity; phylogeny; protein metabolism
Abstract:
... Heterotrimeric G-proteins mediate many responses of eukaryotic cells to external stimuli and have been shown to be important for fungal pathogenicity. In this study, we explored the accumulation of G-protein subunits of the chestnut blight fungus, Cryphonectria parasitica, in mutant strains deleted for one or more putative partner subunits. Using a series of extraction buffers and immunoblot end-p ...
DOI:
10.1007/s00294-003-0367-6

34. Analysis of population structure of the chestnut blight fungus based on vegetative incompatibility genotypes

Author:
Milgroom, M.G.; Cortesi, P.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1999 v.96 no.18 pp. 10518-10523
ISSN:
0027-8424
Subject:
Cryphonectria parasitica; gene frequency; loci; asexual reproduction; genetic variation; alleles; sexual reproduction; fungal anatomy; genotype; genetic recombination; population; Maryland; West Virginia; New York; Italy; Switzerland
Abstract:
... Vegetative incompatibility is a self/nonself recognition system in fungi that has often been used for describing phenotypic diversity in fungal populations. A common hypothesis is that vegetative incompatibility polymorphisms are maintained by balancing selection. However, understanding the evolutionary significance of vegetative incompatibility and the factors that maintain these polymorphisms ha ...
DOI:
10.1073/pnas.96.18.10518
PubMed:
10468641
PubMed Central:
PMC17921

35. Antifungal activity of different Xenorhabdus and Photorhabdus species against various fungal phytopathogens and identification of the antifungal compounds from X. szentirmaii

Author:
Harun Cimen; Mustapha Touray; Sebnem Hazal Gulsen; Omer Erincik; Sebastian L. Wenski; Helge B. Bode; David Shapiro-Ilan; Selcuk Hazir
Source:
Applied microbiology and biotechnology 2021 v.105 no.13 pp. 5517-5528
ISSN:
0175-7598
Subject:
Cryphonectria parasitica; Fusarium oxysporum; Heterorhabditis; Photorhabdus; Sclerotinia sclerotiorum; Steinernema; Thanatephorus cucumeris; Xenorhabdus; antifungal agents; antifungal properties; biological control; biotechnology; chestnut blight; commercialization; fungicides; heat stability; hemocoel; insects; nonribosomal peptides; phytotoxicity; plant pathogenic fungi; polyketide synthases; symbionts
Abstract:
... Xenorhabdus and Photorhabdus spp. are enteric bacterial symbionts of Steinernema and Heterorhabditis nematodes, respectively. These bacteria produce an extensive set of natural products (NPs) with antibacterial, antifungal, antiprotozoal, insecticidal, or other bioactivities when vectored into insect hemocoel by nematodes. We assessed the in vitro activity of different Xenorhabdus and Photorhabdus ...
DOI:
10.1007/s00253-021-11435-3

36. Antimicrobial Activity of Chestnut Extracts for Potential Use in Managing Soilborne Plant Pathogens

Author:
Hao Jianjun J.; Liu Huawei; Donis-Gonzalez Irwin Ronaldo; Lu Xiao Hong; Jones A. Daniel; Fulbright Dennis W.
Source:
Plant disease 2012 v.96 no.3 pp. 354-360
ISSN:
0191-2917
Subject:
Castanea crenata; Castanea sativa; Cryphonectria parasitica; Phytophthora cambivora; Pseudomonas fluorescens; Streptomyces scabiei; agar; anti-infective agents; anti-infective properties; culture media; flavonols; glycosides; greenhouses; growth retardation; leaves; microorganisms; molecular weight; plant pathogens; radishes; temperature; terpenoids; trees
Abstract:
... Chestnut extracts were studied for antimicrobial activity against selected microorganisms, including plant pathogens. Chestnut extract on paper discs was applied to an agar medium to evaluate the inhibition to multiple microorganisms or the extract was added at various concentrations to a culture medium to evaluate the growth of target microorganisms. Chestnut type, tissue of plants (shell, pellic ...
DOI:
10.1094/PDIS-03-11-0169

37. Application of Biotechnology in the Conservation of the Genus Castanea

Author:
Elena Corredoira; Mª Teresa Martínez; Mª José Cernadas; Mª Carmen San José
Source:
Forests 2017 v.8 no.10 pp. -
ISSN:
1999-4907
Subject:
Castanea; Cryphonectria parasitica; Phytophthora; biotechnology; chestnuts; cold storage; cryopreservation; fungi; hardwood forests; in vitro culture; nut crops; pollution; social factors; temperate zones; vegetative propagation; wood
Abstract:
... Castanea is a hardwood forest genus of considerable agro-economic importance for both timber and nut production. Chestnuts are one of the most significant nut crops in the temperate zone. However, this species is threatened by pollution, social factors, economical changes, and two major fungal diseases: ink disease (Phytophthora spp.), and chestnut blight canker (Cryphonectria parasitica). Similar ...
DOI:
10.3390/f8100394

38. Application of airlift bioreactors to accelerate genetic transformation in American chestnut

Author:
Lisheng Kong; Christine T. Holtz; Campbell J. Nairn; Haley Houke; William A. Powell; Kathleen Baier; Scott A. Merkle
Source:
Plant cell, tissue, and organ culture 2014 v.117 no.1 pp. 39-50
ISSN:
0167-6857
Subject:
2,4-D; Agrobacterium; Castanea dentata; Castanea mollissima; Cryphonectria parasitica; air flow; backcrossing; bioreactors; cell proliferation; genetic transformation; genotype; hybrids; labor; reporter genes; sucrose; woody plants
Abstract:
... Airlift bioreactors (ALBs) were constructed and tested for their potential to enhance chestnut embryogenic tissue proliferation for genetic transformation and mass propagation. Multiple genotypes of American chestnut (Castanea dentata) and backcross hybrids of American chestnut and Chinese chestnut (C. dentata × Castanea mollissima) were cultured in ALBs. Medium for tissue proliferation in ALBs wa ...
DOI:
10.1007/s11240-013-0418-8

39. Application of biological control of chestnut blight on a nationwide scale in Greece: results and prospects

Author:
S. Diamandis; C. Perlerou; Z. Nakopoulou; V. Christopoulos; E. Topalidou; G. Tziros
Source:
Acta horticulturae 2014 no.1043 pp. 23-34
ISSN:
0567-7572
Subject:
Castanea; Cryphonectria parasitica; biological control; coppicing; forests; fungi; horticulture; human resources; inoculum; orchards; silvicultural practices; tree age; viruses; Greece
Abstract:
... Since 1963 when chestnut blight was recorded for the first time in Greece, the disease has spread into all 29 chestnut growing prefectures. As a result the national, annual chestnut production was reduced from 18,000 tons in the 1960s to 11,000 tons in 2005. A project for the application of biological control on a nationwide scale was funded and implemented during the period 2007-2009. Hypovirulen ...

40. Assessing the Phytosanitary Risk Posed by an Intraspecific Invasion of Cryphonectria parasitica in Europe

Author:
Francesca Dennert; Joana Beatrice Meyer; Daniel Rigling; Simone Prospero
Source:
Phytopathology 2019 v.109 no.12 pp. 2055-2063
ISSN:
0031-949X
Subject:
Castanea sativa; Cryphonectria parasitica; agar; genotype; greenhouses; invasive species; mortality; plant pathogens; risk; seedlings; spores; sporulation; temperature; virulence; South Korea; Switzerland; United States
Abstract:
... Intraspecific cryptic invasions may occur when new strains of an invasive species are introduced into an area where this species had already been introduced previously. In plant pathogens, such invasions are not well studied, even if, potentially, they can have severe consequences. Here, we investigated the effects of a potential intraspecific invasion in Europe of Cryphonectria parasitica, the ca ...
DOI:
10.1094/PHYTO-06-19-0197-R

41. Assessment of hypovirulent isolates of Cryphonectria parasitica for potential in biological control of chestnut blight

Author:
Melzer, M.S.; Dunn, M.; Zhou, T.; Boland, G.J.
Source:
Canadian journal of plant pathology 1997 v.19 no.1 pp. 69-77
ISSN:
0706-0661
Subject:
phenotype; double-stranded RNA; assays; virulence; plant pathogenic fungi; inheritance (genetics); strains; Cryphonectria parasitica; mycelium; Ontario
Abstract:
... Hypovirulent isolates of Cryphonectria parasitica were evaluated for virulence, conversion capacity, percent transmission of hypovirulent phenotype to conidia, and hybridization relationships among double-stranded RNA (dsRNA) associated with hypovirulent isolates. Significant differences in lesion diameters were detected among hypovirulent isolates in comparison to known virulent and hypovirulent ...
DOI:
10.1080/07060669709500576

42. Assessment of the core cryparin promoter from Cryphonectria parasitica for heterologous expression in filamentous fungi

Author:
Bo-Ra Kwon; Myoung-Ju Kim; Jin-A Park; Hea-Jong Chung; Jung-Mi Kim; Seung-Moon Park; Sung-Hwan Yun; Moon-Sik Yang; Dae-Hyuk Kim
Source:
Applied microbiology and biotechnology 2009 v.83 no.2 pp. 339-348
ISSN:
0175-7598
Subject:
Aspergillus nidulans; Aspergillus niger; Cryphonectria parasitica; Fusarium graminearum; chimerism; fungi; glucose oxidase; glyceraldehyde-3-phosphate dehydrogenase; heterologous gene expression; hydrophobins; hygromycin B; laccase; promoter regions; quantitative polymerase chain reaction; reporter genes; start codon; transcription (genetics)
Abstract:
... Cryparin is an abundant cell-wall-associated hydrophobin of Cryphonectria parasitica. Although cryparin is encoded as a single copy gene, it is the most abundant protein produced by this fungus when grown in liquid culture. Studies to characterize the transcriptional regulatory element(s) found that the fragment between nt −188 and the start codon was the minimal but sufficient promoter element fo ...
DOI:
10.1007/s00253-009-1906-y
PubMed:
19238380

43. Attenuation of fungal virulence by synthetic infectious hypovirus transcripts

Author:
Chen, B.; Choi, G.H.; Nuss, D.L.
Source:
Science 1994 v.264 no.5166 pp. 1762-1764
ISSN:
0036-8075
Subject:
Cryphonectria parasitica; plant viruses; virulence; pathogenesis; mycoviruses
Abstract:
... Noninfectious, cytoplasmically transmissible viral double-stranded RNAs of the genus Hypovirus cause reduced virulence (hypovirulence) in the chestnut blight fungus Cryphonectria parasitica, providing the basis for virus-mediated biological control of a fungal disease. Synthetic transcripts corresponding to a full-length hypovirus RNA coding strand are infectious when introduced into fungal sphero ...
DOI:
10.1126/science.8209256
PubMed:
8209256

44. Bacteria found on American chestnut bark and their potential in biocontrol of chestnut blight

Author:
Groome, P.C.; Tattar, T.A.; Mount, M.S.
Source:
Arboricultural journal 2001 v.25 no.3 pp. 221-234
Subject:
Castanea dentata; Cryphonectria parasitica; Bacillus megaterium; bark; biological control; biological control agents; virulence; biological resistance
DOI:
10.1080/03071375.2001.9747307

45. Biocontrol potential of an endophytic Serratia sp. G3 and its mode of action

Author:
Liu, X.; Jia, J.; Atkinson, S.; Cámara, M.; Gao, K.; Li, H.; Cao, J.
Source:
World journal of microbiology & biotechnology 2010 v.26 no.8 pp. 1465-1471
ISSN:
0959-3993
Subject:
Botrytis cinerea; Cryphonectria parasitica; Rhizoctonia cerealis; Serratia; Triticum aestivum; Valsa sordida; antibiotics; antifungal properties; biological control; biological control agents; cell communication; chitinase; endophytes; homoserine; host plants; indole acetic acid; lactones; liquid chromatography; mechanism of action; plant diseases and disorders; plant pathogens; proteinases; quorum sensing; ribosomal DNA; siderophores; stems; synergism; tandem mass spectrometry
Abstract:
... Biological control is being considered as an alternative or a supplemental way of reducing the use of chemicals in agriculture. An endophytic strain G3 with potential as a biocontrol agent was isolated from the stems of Triticum aestivum L. It was classified by 16S rDNA sequencing as a member of Serratia. Strain G3 displayed a broad spectrum of antifungal activity in vitro against a number of phyt ...
DOI:
10.1007/s11274-010-0321-y

46. Biological control of chestnut blight in Croatia: an interaction between host sweet chestnut, its pathogen Cryphonectria parasitica and the biocontrol agent Cryphonectria hypovirus 1

Author:
Ljiljana Krstin; Zorana Katanić; Marin Ježić; Igor Poljak; Lucija Nuskern; Ivana Matković; Marilena Idžojtić; Mirna Ćurković‐Perica
Source:
Pest management science 2017 v.73 no.3 pp. 582-589
ISSN:
1526-498X
Subject:
Castanea; Cryphonectria hypovirus 1; Cryphonectria parasitica; biological control; biological control agents; forests; fungi; genotype; orchards; pathogens; stems; transfection; trees; viruses; Croatia
Abstract:
... BACKGROUND: Chestnut blight, caused by the fungus Cryphonectria parasitica, is a severe chestnut disease that can be controlled with naturally occurring hypoviruses in many areas of Europe. The aim of this research was to measure the effect of different Cryphonectria hypovirus 1 (CHV1) strains on the growth of the fungal host and select strains that could potentially be used for human‐mediated bio ...
DOI:
10.1002/ps.4335

47. Biological control of chestnut blight in Portugal

Author:
L. M. Martins; J. P. Castro; M. E. Gouveia
Source:
Acta horticulturae 2014 no.1043 pp. 51-56
ISSN:
0567-7572
Subject:
Castanea sativa; Cryphonectria parasitica; biological control; disease control; founder effect; fungi; health status; pathogens; plant health; population structure; quarantine; selfing; soil management; soil types; tree age; trees; virulence; Portugal
Abstract:
... Hypovirulence is a specific biological control method of chestnut blight, a lethal disease of American and European chestnut. The causal pathogen of chestnut blight is Cryphonectria parasitica, a fungus of Asian origin and an A2 quarantine organism in Europe. The disease has been reported since 1990 in Portugal, one of the last European countries where the pathogen was introduced. The chestnut bli ...

48. Breeding blight resistant American chestnut for Canada

Author:
Dale, A.; Galic, D.
Source:
Acta horticulturae 2014 no.1019 pp. 49-53
ISSN:
0567-7572
Subject:
Castanea dentata; Cryphonectria parasitica; blight; breeding; open pollination; planting; trees; Connecticut; Ontario
Abstract:
... Southern Ontario is the northern end of the range of American chestnut (Castanea dentate). Since 2000, we have been breeding American chestnut with resistance to chestnut blight (Cryphonectria parasitica). Initially, two populations of trees were produced: 1) with extant Canadian C. dentate trees hybridized with partially resistant trees from Connecticut; and 2) with extant Canadian C. dentate tre ...

49. Building a foundation: Land-use history and dendrochronology reveal temporal dynamics of a Tsuga canadensis (Pinaceae) forest

Author:
Ellison Aaron M.; Lavine Michael; Kerson Peter B.; Barker Plotkin Audrey A.; Orwig David A.
Source:
Rhodora 2014 v.116 no.968 pp. 377-427
ISSN:
0035-4902
Subject:
Acer rubrum; Betula lenta; Castanea dentata; Cryphonectria parasitica; Lymantria dispar; Pinus strobus; Quercus rubra; Tsuga canadensis; age structure; dendrochronology; drought; forest ecology; forests; hurricanes; insects; land use; logging; models; pathogens; stand structure; temporal variation; tree growth; trees; Massachusetts; New England region
Abstract:
... We used historical documents, stand mapping, and new methods of dendrochronological analysis to reconstruct 250 y of land-use history of the Simes Tract in Petersham, MA. These data were then used to interpret the origin of the current forest's stand structure within the experimental plots of the Harvard Forest Hemlock Removal Experiment within the Simes Tract, an experiment that examines the effe ...
DOI:
10.3119/14-04

50. CHV3-type dsRNAs and the GH2 genotype in a population of Cryphonectria parasitica in Ontario

Author:
Melzer, M.S.; Boland, G.J.
Source:
Canadian journal of plant pathology 1999 v.21 no.3 pp. 248-255
ISSN:
0706-0661
Subject:
Castanea dentata; phenotype; double-stranded RNA; biological control agents; strain differences; clones; genotype; DNA fingerprinting; population; Cryphonectria parasitica; virulence; biological control
Abstract:
... CCP19, a hypovirulent isolate of Cryphonectria parasitica collected from a site near Bothwell, Ontario, in 1987 has shown potential for use in biological control of chestnut blight. CCP19 contains dsRNA that hybridizes with hypovirus CHV3 from isolate GH2 (CHV3-GH2), a hypovirulent isolate of C. parasitica from Michigan. In 1995, 26 isolates of C. parasitica were collected at the Bothwell site, an ...
DOI:
10.1080/07060669909501187

51. CYP1, a hypovirus-regulated cyclophilin, is required for virulence in the chestnut blight fungus

Author:
CHEN, MIN-MEI; JIANG, MINGGUO; SHANG, JINJIE; LAN, XIUWAN; YANG, FENG; HUANG, JINGKUAN; NUSS, DONALD L.; CHEN, BAOSHAN
Source:
Molecular plant pathology 2011 v.12 no.3 pp. 239-246
ISSN:
1464-6722
Subject:
Castanea; Cryphonectria parasitica; G-proteins; Hypovirus; cyclophilins; cyclosporine; genes; mutants; plant pathogenic fungi; stems; virulence
Abstract:
... Cyclophilins are peptidyl-prolyl cis-trans isomerases that are highly conserved throughout eukaryotes and are the cellular target of the immunosuppressive drug cyclosporin A (CsA). We cloned cyp1, a cyclophilin A-encoding gene in the phytopathogenic fungus Cryphonectria parasitica, and showed that this gene was downregulated following infection by a virulence-attenuating hypovirus. The function of ...
DOI:
10.1111/j.1364-3703.2010.00665.x
PubMed:
21355996
PubMed Central:
PMC3313458

52. Canker Stain: A Lethal Disease Destroying Iconic Plane Trees

Author:
Tsopelas Panaghiotis; Santini Alberto; Wingfield Michael J.; Wilhelm de Beer Z.
Source:
Plant disease 2017 v.101 no.5 pp. 645-658
ISSN:
0191-2917
Subject:
Ceratocystis; Cryphonectria parasitica; Dutch elm disease; dieback; ecosystems; fungi; mortality; ornamental trees; pathogens; risk; tree diseases; Asia; France; Greece; Italy; North America; Spain; Switzerland
Abstract:
... In Europe, both Oriental plane and London plane trees are seriously threatened by the invasive fungal pathogen Ceratocystis platani (Walter) Engelbr. & T.C. Harr., the causal agent of canker stain disease (CSD) of plane trees. The fungus is considered to be indigenous to North America and was accidently introduced into Europe during World War II, where it continues to spread clonally. The impact o ...
DOI:
10.1094/PDIS-09-16-1235-FE

53. Castanea sativa: genotype-dependent recovery from chestnut blight

Author:
Marin Ježić; Ljiljana Krstin; Igor Poljak; Zlatko Liber; Marilena Idžojtić; Marija Jelić; Jasenka Meštrović; Marko Zebec; Mirna Ćurković-Perica
Source:
Tree genetics & genomes 2014 v.10 no.1 pp. 101-110
ISSN:
1614-2942
Subject:
Castanea sativa; Cryphonectria hypovirus 1; Cryphonectria parasitica; callus; forests; fungi; genotype; genotyping; necrosis; trees; virulence; Croatia
Abstract:
... In Lovran (coastal Croatia), a unique forest/orchard of evenly mixed grafted marrons and naturally growing nongrafted sweet chestnut trees exists. This old chestnut population has been devastated by chestnut blight, caused by an aggressive introduced pathogenic fungus, Cryphonectria parasitica. However, initial observations indicated recovery of naturally growing chestnut trees in that area, media ...
DOI:
10.1007/s11295-013-0667-z

54. Cause of withering of Staghorn Sumach (Rhus typhina L.) in selected localities in Slovakia

Author:
Andrea Čerevková; Katarína Adamčíková; Marek Kobza; Gabriela Juhásová
Source:
Acta Societatis Botanicorum Poloniae 2005 v.74 no.1 pp. 29-33
ISSN:
0001-6977
Subject:
Castanea sativa; Cryphonectria parasitica; Fusarium oxysporum; Neofusicoccum ribis; Quercus; Rhus typhina; agar; fungi; hyphae; pests; pycnidia; quarantine; Slovakia
Abstract:
... Health state of Rhus typhina was evaluated in 59 localities and 74 sites over the years 2001-2003 with scope (1) to identify the parasitic mycoflora of R. typhina in Slovakia and (2) to identify the occurrence of Cryphonectria parasitica, declared as an quarantine pest by the EPPO, besides the chestnut (Castanea sativa Mill.) and oak (Quercus sp.) also on R. typhina. The fungus Botryosphaeria ribi ...
DOI:
10.5586/asbp.2005.006

55. Changes in Cryphonectria parasitica Populations Affect Natural Biological Control of Chestnut Blight

Author:
Marin Ježić; Jelena Mlinarec; Rosemary Vuković; Zorana Katanić; Ljiljana Krstin; Lucija Nuskern; Igor Poljak; Marilena Idžojtić; Mirta Tkalec; Mirna Ćurković-Perica
Source:
Phytopathology 2018 v.108 no.7 pp. 870-877
ISSN:
0031-949X
Subject:
Castanea sativa; Cryphonectria hypovirus 1; Cryphonectria parasitica; biological control; disease control; disease course; fungi; indigenous species; invasive species; landscapes; monitoring; mycelium; plant pathogens; plants (botany); virulence; viruses
Abstract:
... Invasive species, especially plant pathogens, have a potential to completely eradicate native plant species and remodel landscapes. Tripartite interactions among sweet chestnut (Castanea sativa), chestnut blight-causing invasive fungus Cryphonectria parasitica, and hyperparasitic virus Cryphonectria hypovirus 1 (CHV1) were studied in two populations. The number of different vegetative compatibilit ...
DOI:
10.1094/PHYTO-07-17-0252-R

56. Characterisation and pathogenicity of Cryphonectria parasitica on sweet chestnut and sessile oak trees in Serbia

Author:
Dragan Karadžić; Zlatan Radulović; Katarzyna Sikora; Zoran Stanivuković; Vesna Golubović Ćurguz; Tomasz Oszako; Ivan Milenković
Source:
Plant Protection Science v.55 no.3 pp. 191-201
ISSN:
1212-2580
Subject:
Castanea; Cryphonectria parasitica; Hypovirus; Quercus petraea; bark; fungi; pathogenicity; plant protection; Serbia
Abstract:
... The presence of Cryphonectria parasitica (Murrill) M.E. Barr was studied in six natural and planted stands of sweet chestnut in Serbia. The fungus was detected on the sweet chestnut in five localities and on the sessile oak in one locality. In total, 77 isolates from the sweet chestnut and five isolates from the sessile oak were obtained. Based on the culture morphology, all the obtained isolates ...
DOI:
10.17221/38/2018-pps

57. Characteristics of the Cryphonectria parasitica isolated from Quercus in Slovakia

Author:
Adamčíková, K.; Kobza, M.; Juhásová, G.
Source:
Forest pathology 2010 v.40 no.5 pp. 443-449
ISSN:
1437-4781
Subject:
Castanea sativa; Cryphonectria parasitica; Quercus robur; chestnuts; disease incidence; field experimentation; mixed forests; stems; trees; virulence; Slovakia
Abstract:
... The occurrence of chestnut blight (Cryphonectria parasitica) on oaks in mixed chestnut-oak forests was studied in 2003-2008 in Slovakia. Infections on living Quercus trees were found at four of seven localities. The disease incidence on oaks ranged from 1.3% to 15.8%. The symptoms on infected oaks were similar to those on chestnut, but less conspicuous. Cankers of C. parasitica were found only on ...
DOI:
10.1111/j.1439-0329.2009.00618.x

58. Characterization of CpSte11, a MAPKKK gene of Cryphonectria parasitica, and initial evidence of its involvement in the pheromone response pathway

Author:
JIN‐AH PARK; JUNG‐MI KIM; SEUNG‐MOON PARK; DAE‐HYUK KIM
Source:
Molecular plant pathology 2012 v.13 no.3 pp. 240-250
ISSN:
1464-6722
Subject:
transcription factors; phenotype; pigmentation; yeasts; glycerol; pheromones; female fertility; gene targeting; genes; mutants; Castanea; mitogen-activated protein kinase kinase kinase; Cryphonectria parasitica; virulence; mitogen-activated protein kinase
Abstract:
... The gene CpSte11 of Cryphonectria parasitica, which encodes a yeast Ste11 homologue, was cloned and characterized. Gene replacement analysis revealed a high frequency of CpSte11 null mutants. When compared with the wild‐type parent strain, CpSte11 null mutants showed no difference in terms of growth rate or pigmentation. However, CpSte11 null mutants showed a marked decrease in both the number and ...
DOI:
10.1111/j.1364-3703.2011.00742.x
PubMed:
21895942

59. Characterization of Cryphonectria parasitica isolates collected from Aydin Province in Turkey

Author:
Erincik, O.; Doken, M.T.; Acikgoz, S.; Ertan, E.
Source:
Phytoparasitica 2008 v.36 no.3 pp. 249-259
ISSN:
0334-2123
Subject:
double-stranded RNA; microbial genetics; conidia; fungal diseases of plants; strain differences; cankers (plants); chestnuts; Castanea sativa; Cryphonectria parasitica; virulence; disease surveillance; disease incidence; Turkey (country)
DOI:
10.1007/BF02980771

60. Characterization of South African Cryphonectria cubensis isolates infected with a C. parasitica hypovirus

Author:
Van Heerden, S.W.; Geletka, L.M.; Preisig, O.; Nuss, D.L.; Wingfield, B.D.; Wingfield, M.J.
Source:
Phytopathology 2001 v.91 no.7 pp. 628-632
ISSN:
0031-949X
Subject:
plant viruses; plant diseases and disorders; Eucalyptus; disease control; plant pathogenic fungi; Cryphonectria parasitica; virulence; biological control; South Africa
Abstract:
... Cryphonectria cubensis is the causal agent of a serious canker disease of Eucalyptus spp. in tropical and subtropical parts of the world. In this study, a South African C. cubensis isolate was transfected by electroporation with a synthetic RNA transcript corresponding to the full-length coding strand of the C. parasitica hypovirus (CHV1-EP713). Hypovirus infection resulted in pronounced morpholog ...
DOI:
10.1094/PHYTO.2001.91.7.628
PubMed:
18942991

61. Characterization of a Hypovirus-Regulated Septin Cdc11 Ortholog, CpSep1, from the Chestnut Blight Fungus Cryphonectria parasitica

Author:
Myeongjin Jo; Kum-Kang So; Yo-Han Ko; Jeesun Chun; Jung-Mi Kim; Dae-Hyuk Kim
Source:
Molecular plant-microbe interactions 2019 v.32 no.3 pp. 286-295
ISSN:
0894-0282
Subject:
Aspergillus nidulans; Castanea; Cryphonectria hypovirus 1; Cryphonectria parasitica; Saccharomyces cerevisiae; apples; bark; biotin; conidia; culture media; fungi; gene expression regulation; genes; germination; growth retardation; hyphae; mutants; mycelium; pigmentation; sorbitol; stems; tannins; virulence
Abstract:
... We identified a protein spot showing downregulation in the presence of Cryphonectria hypovirus 1 and tannic acid supplementation as a septin subunit with the highest homology to the Aspergillus nidulans aspA gene, an ortholog of the Saccharomyces cerevisiae Cdc11 gene. To analyze the functional role of this septin component (CpSep1), we constructed its null mutant and obtained a total of eight CpS ...
DOI:
10.1094/MPMI-07-18-0194-R

62. Characterization of an endophytic whorl-forming Streptomyces from Catharanthus roseus stems producing polyene macrolide antibiotic

Author:
Erick Francisco Rakotoniriana; Gabrielle Chataigné; Guy Raoelison; Christian Rabemanantsoa; Françoise Munaut; Mondher El Jaziri; Suzanne Urveg-Ratsimamanga; Jacqueline Marchand-Brynaert; Anne-Marie Corbisier; Stéphane Declerck; Joëlle Quetin-Leclercq
Source:
Canadian journal of microbiology 2012 v.58 no.5 pp. 617-627
ISSN:
1480-3275
Subject:
Catharanthus roseus; Cladosporium cucumerinum; Cryphonectria parasitica; Epidermophyton floccosum; Fusarium oxysporum; Glomerella cingulata; Pyrenophora tritici-repentis; Sclerotinia sclerotiorum; Streptomyces cinnamoneus; Thanatephorus cucumeris; Trichophyton rubrum; antibiotics; antifungal agents; antifungal properties; endophytes; fungi; macrolides; new subspecies; nuclear magnetic resonance spectroscopy; nucleotide sequences; pathogens; phylogeny; ribosomal DNA; sequence homology; stems; thin layer chromatography
Abstract:
... An endophytic whorl-forming Streptomyces sp. designated as TS3RO having antifungal activity against a large number of fungal pathogens, including Sclerotinia sclerotiorum, Rhizoctonia solani, Colletotrichum gloeosporioides, Cryphonectria parasitica, Fusarium oxysporum, Pyrenophora tritici-repentis, Epidermophyton floccosum, and Trichophyton rubrum, was isolated from surface-sterilized Catharanthus ...
DOI:
10.1139/w2012-034

63. Characterization of hypovirulent isolates of the chestnut blight fungus, Cryphonectria parasitica from the Marmara and Black Sea regions of Turkey

Author:
Seçil Akıllı; Çiğdem Ulubaş Serçe; Yakup Zekai Katırcıoğlu; Salih Maden; Daniel Rigling
Source:
European journal of plant pathology 2013 v.135 no.2 pp. 323-334
ISSN:
0929-1873
Subject:
Castanea sativa; Cryphonectria hypovirus 1; Cryphonectria parasitica; Marmara; agar; biological control; double-stranded RNA; fungi; genome; monophenol monooxygenase; reverse transcriptase polymerase chain reaction; tannins; viruses; Black Sea
Abstract:
... Chestnut blight caused by the introduced fungus Cryphonectria parasitica has been responsible for the decline of Castanea sativa in Turkey since the 1960s. In this study, 72 C. parasitica isolates were recovered from the Marmara and Black Sea regions of Turkey showing white or cream-coloured culture morphology and were subjected to various tests to determine if they were infected by Cryphonectria ...
DOI:
10.1007/s10658-012-0089-z

64. Characterization of mutants of the chestnut blight fungus (Cryphonectria parasitica) with unusual hypovirus symptoms

Author:
Faruk, Md. Iqbal; Izumimoto, Masatoshi; Suzuki, Nobuhiro
Source:
Journal of general plant pathology 2008 v.74 no.6 pp. 425-433
ISSN:
1345-2630
Subject:
Cryphonectria hypovirus 1; Cryphonectria parasitica; apples; complementary DNA; fungi; genetic techniques and protocols; glucose; hosts; mutagenicity; mutants; phenotype; pigmentation; screening; sporulation; vegetative growth; virulence; viruses
Abstract:
... The type virus of the family Hypoviridae, Cryphonectria hypovirus 1 strain EP713 (CHV1-EP713), infects Cryphonectria parasitica, the filamentous causal fungus of chestnut blight, and reduces its virulence. This pathosystem serves as a model to study fungus-mycovirus interactions. We previously developed a genetic screening protocol for host factors associated with symptom induction by CHV1-EP713 a ...
DOI:
10.1007/s10327-008-0121-5

65. Characterization of the Papain-Like Protease p29 of the Hypovirus CHV1-CN280 in Its Natural Host Fungus Cryphonectria parasitica and Nonhost Fungus Magnaporthe oryzae

Author:
Qin Xiong; Linqiao Zhang; Justin Waletich; Linlin Zhang; Chen Zhang; Xinyue Zheng; Yulin Qian; Zhengguang Zhang; Yuanchao Wang; Qiang Cheng
Source:
Phytopathology 2019 v.109 no.5 pp. 736-747
ISSN:
0031-949X
Subject:
Cryphonectria hypovirus 1; Cryphonectria parasitica; Magnaporthe oryzae; amino acids; biological control; blast disease; enzyme activity; heterologous gene expression; laccase; mycelium; nucleotide sequences; pathogenicity; peroxidase; phenotype; pigmentation; plant pathogenic fungi; proteinases; sporulation; China
Abstract:
... Cryphonectria hypovirus 1 strain CN280 (CHV1-CN280) was isolated from North China and exhibited typical hypovirulence-associated traits. We previously reported that CHV1-CN280 was more aggressive and had a higher horizontal transmission ability between Cryphonectria parasitica isolates belonging to different vegetative compatibility groups than two other CHV1 hypoviruses (namely, CHV1-EP713 and CH ...
DOI:
10.1094/PHYTO-08-18-0318-R

66. Characterization of the non-coagulating enzyme fraction of different milk-clotting preparations

Author:
Odile Rolet-Répécaud; Françoise Berthier; Eric Beuvier; Stéphane Gavoye; Eric Notz; Sébastien Roustel; Valérie Gagnaire; Christine Achilleos
Source:
Lebensmittel-Wissenschaft + Technologie 2013 v.50 no.2 pp. 459-468
ISSN:
0023-6438
Subject:
Cryphonectria parasitica; Rhizomucor miehei; ammonia; bovine serum albumin; calves; chymosin; fungi; manufacturing; milk clotting; pH; polypeptides; protein composition; proteolysis; rennet; sodium chloride; traditional technology
Abstract:
... Proteolytic milk-clotting enzymes are extracted from various sources (animals, plants, fungi) and processed according to various methods that are specific to each manufacturer or cheese-maker. Chemical composition and polypeptide patterns of 24 milk-clotting preparations from animal and fungal sources: 10 commercial rennets, 9 artisanal calf rennets, 2 recombinant chymosin preparations and 3 micro ...
DOI:
10.1016/j.lwt.2012.08.021

67. Chestnut Breeding in the United States for Disease and Insect Resistance

Author:
Anagnostakis Sandra L.
Source:
Plant disease 2012 v.96 no.10 pp. 1392-1403
ISSN:
0191-2917
Subject:
Castanea dentata; Castanea sativa; Cryphonectria parasitica; Phytophthora cinnamomi; Vitis; birds; breeding; chestnuts; cultivars; death; insects; mountains; pathogens; rural families; temperate zones; trade; trees; wildlife; wood; Connecticut; Italy; Portugal; Southeastern United States; Spain; Turkey (country)
Abstract:
... The genus Castanea (family Fagaceae) is found in north temperate climates around the world, and is highly prized in many different cultures for its nutritious nuts and valuable timber. Selection for larger, better-tasting nuts has been ongoing in Asia and Europe for centuries. Early trade routes moved European chestnut trees (C. sativa) west of their native range (in the Caucasus mountains), and t ...
DOI:
10.1094/PDIS-04-12-0350-FE

68. Chestnut Leaf Inoculation Assay as a Rapid Predictor of Blight Susceptibility

Author:
Newhouse Andrew E.; Spitzer Jesse E.; Maynard Charles A.; Powell William A.
Source:
Plant disease 2014 v.98 no.1 pp. 4-9
ISSN:
0191-2917
Subject:
Castanea dentata; Cryphonectria parasitica; blight; chestnuts; fungi; leaves; screening; seedlings; stems; trees; virulence; North America
Abstract:
... American chestnuts (Castanea dentata), effectively eliminated from eastern North America by chestnut blight in the twentieth century, are the subject of multiple restoration efforts. Screening individual trees (or tree types) for blight resistance is a critical step in all of these programs. Traditional screening involves inoculating stems of >3-year-old trees with the blight fungus (Cryphonectria ...
DOI:
10.1094/PDIS-01-13-0047-RE

69. Chestnut bark tannin assays and growth of chestnut blight fungus on extracted tannin

Author:
Anagnostakis, S.L.
Source:
Journal of chemical ecology 1992 v.18 no.8 pp. 1365-1373
ISSN:
0098-0331
Subject:
Cryphonectria parasitica; Castanea; bark; plant extracts; tannins; bioassays; disease resistance
Abstract:
... Tannins extracted from the green bark of each of two Chinese, Japanese, and American chestnut trees were assayed in a protein-binding test. Four levels of tannins were added to a buffered, minimal growth medium, and a standard, virulent strain of the chestnut blight fungus was grown. There were only slight differences in protein binding between the extracts from different species. Fungal growth wa ...
DOI:
10.1007/BF00994362
PubMed:
24254212

70. Chestnut blight fungus in Croatia: diversity of vegetative compatibility types, mating types and genetic variability of associated Cryphonectria hypovirus 1

Author:
Krstin, L.; Novak-Agbaba, S.; Rigling, D.; Krajačić, M.; Ćurković Perica, M.
Source:
Plant pathology 2008 v.57 no.6 pp. 1086-1096
ISSN:
0032-0862
Subject:
Castanea; Cryphonectria hypovirus 1; Cryphonectria parasitica; evolution; fungi; genetic variation; mating types; perithecia; restriction fragment length polymorphism; sexual reproduction; virulence; Croatia
Abstract:
... In order to improve understanding of its diversity, 338 isolates of Cryphonectria parasitica, the causal agent of chestnut blight, were sampled from 10 chestnut populations throughout chestnut-growing coastal and continental areas of Croatia. Eighteen vegetative compatibility (VC) types were identified. The VC type EU-1 was the most widespread, comprising 42·9% of the isolates, followed by EU-2 (2 ...
DOI:
10.1111/j.1365-3059.2008.01905.x

71. Chestnuts bred for blight resistance depart nursery with distinct fungal rhizobiomes

Author:
Christopher Reazin; Richard Baird; Stacy Clark; Ari Jumpponen
Source:
Mycorrhiza 2019 v.29 no.4 pp. 313-324
ISSN:
0940-6360
Subject:
Castanea dentata; Castanea mollissima; Cryphonectria parasitica; backcrossing; blight; chestnuts; disease resistance; ectomycorrhizae; endophytes; fungal communities; fungi; hybrids; pathogens; plant establishment; roots; saprophytes; seedlings; soil; soil ecology
Abstract:
... Restoration of the American chestnut (Castanea dentata) is underway using backcross breeding that confers chestnut blight disease resistance from Asian chestnuts (most often Castanea mollissima) to the susceptible host. Successful restoration will depend on blight resistance and performance of hybrid seedlings, which can be impacted by below-ground fungal communities. We compared fungal communitie ...
DOI:
10.1007/s00572-019-00897-z

72. Clonal population structure of the chestnut blight fungus in expanding ranges in southeastern Europe

Author:
MILGROOM, MICHAEL G.; SOTIROVSKI, KIRIL; SPICA, DOMENICO; DAVIS, JOANNE E.; BREWER, MARIN T.; MILEV, MILKO; CORTESI, PAOLO
Source:
Molecular ecology 2008 v.17 no.20 pp. 4446-4458
ISSN:
0962-1083
Subject:
founder effect; haplotypes; loci; linkage disequilibrium; clones; random mating; population structure; Cryphonectria parasitica; fungi; Romania; Italy; Greece; Bulgaria
Abstract:
... Expanding populations are often less genetically diverse at their margins than at the centre of a species' range. Established, older populations of the chestnut blight fungus, Cryphonectria parasitica, are more variable for vegetative compatibility (vc) types than in expanding populations in southeastern Europe where C. parasitica has colonized relatively recently. To test whether vc types represe ...
DOI:
10.1111/j.1365-294X.2008.03927.x
PubMed:
18803594

73. Cloning and Expression of the Chitinase Encoded by <i>ChiKJ406136</i> from <i>Streptomyces Sampsonii</i> (Millard & Burr) Waksman KJ40 and Its Antifungal Effect

Author:
Shujiang Li; Boyang Zhang; Hanmingyue Zhu; Tianhui Zhu
Source:
Forests 2018 v.9 no.11 pp. -
ISSN:
1999-4907
Subject:
Castanea mollissima; Cryphonectria parasitica; Cylindrocladium scoparium; Escherichia coli; Eucalyptus robusta; Fusarium oxysporum; Juglans regia; Neofusicoccum parvum; Streptomyces sampsonii; antifungal properties; biological control; biological control agents; chitinase; crude protein; enzyme activity; forest diseases; forest pathogens; forest trees; fungi; genes; imidazole; leaf blight; mycelium; polymerase chain reaction; protein synthesis; recombinant proteins; root rot
Abstract:
... The present study demonstrated that the chitinase gene ChiKJ406136 of Streptomyces sampsonii (Millard & Burr) Waksman KJ40 could be cloned using a PCR protocol and expressed in Escherichia coli (Migula) Castellani & Chalmers BL21 (DE3), and the recombinant protein had antifungal effect on four forest pathogens (Cylindrocladium scoparium Morgan, Cryphonectria parasitica (Murrill) Barr, Neof ...
DOI:
10.3390/f9110699

74. Comparative analysis of the transcriptome of Castanea pumila var. pumila

Author:
Dane, F.; Li, X.; Kocot, K.; Goertzen, L.
Source:
Acta horticulturae 2014 no.1019 pp. 55-60
ISSN:
0567-7572
Subject:
Castanea dentata; Castanea mollissima; Cryphonectria parasitica; biogeography; chloroplast genome; chloroplasts; genes; habitats; hybridization; hypersensitive response; metabolism; plant hormones; transcriptome; transcriptomics
Abstract:
... The biogeographical history of North American Castanea species, C. dentate (American chestnut) and C. pumila var. pumila (Allegheny chinkapin) has been complicated by their distribution in similar habitats in the southern region of the species’ range, occurrence of natural hybridization and incidence of chestnut blight. Transcriptome sequencing of C. pumila var. pumila was initiated for comparativ ...

75. Comparative chloroplast and nuclear DNA analysis of Castanea species in the southern region of the USA

Author:
Xiaowei Li; Fenny Dane
Source:
Tree genetics & genomes 2013 v.9 no.1 pp. 107-116
ISSN:
1614-2942
Subject:
Castanea dentata; Cryphonectria parasitica; chestnuts; chloroplasts; coastal plains; haplotypes; hybridization; hybrids; mutation; nuclear genome; phylogeography; provenance; trees; Alabama; Appalachian region
Abstract:
... Boundaries between American Castanea species (Castanea dentata, the American chestnut and C. pumila var. pumila, the Allegheny chinkapin, and var. ozarkensis, the Ozark chinkapin) have been difficult to establish because of intraspecific variation, interspecific similarities and the incidence of chestnut blight, which has prevented trees from maturing. In this study, informative chloroplast (cp) D ...
DOI:
10.1007/s11295-012-0538-z

76. Comparative efficacy of gypsy moth (Lepidoptera: Erebidae) entomopathogens on transgenic blight‐tolerant and wild‐type American, Chinese, and hybrid chestnuts (Fagales: Fagaceae)

Author:
Aaron J. Brown; Andrew E. Newhouse; William A. Powell; Dylan Parry
Source:
Insect science 2020 v.27 no.5 pp. 1067-1078
ISSN:
1672-9609
Subject:
Bacillus thuringiensis subsp. kurstaki; Castanea dentata; Cryphonectria parasitica; Erebidae; Lymantria dispar; Lymantria dispar multiple nucleopolyhedrovirus; dosage; ecological competition; entomopathogens; forests; fungi; genetic engineering; genetic transformation; genetically modified organisms; genotype; hardwood; hybrids; insect larvae; insects; leaves; marshes; mortality; nontarget organisms; phenotypic plasticity; transgenes; trees; water; North America
Abstract:
... American chestnut (Castanea dentata [Marsh.] Borkh.) was once the dominant hardwood species in Eastern North America before an exotic fungal pathogen, Cryphonectria parasitica (Murrill) Barr, functionally eliminated it across its range. One promising approach toward restoring American chestnut to natural forests is development of blight‐tolerant trees using genetic transformation. However, transfo ...
DOI:
10.1111/1744-7917.12713

77. Comparative proteomic analysis of chestnut blight fungus, Cryphonectria parasitica, under tannic-acid-inducing and hypovirus-regulating conditions

Author:
Jung-Mi Kim; Jin-Ah Park; Dae-Hyuk Kim
Source:
Canadian journal of microbiology 2012 v.58 no.7 pp. 863-871
ISSN:
1480-3275
Subject:
Castanea; Cryphonectria parasitica; bark; cell communication; fungi; genes; glutathione transferase; heat shock proteins; mycelium; pathogenicity; proteomics; spectroscopy; structural proteins; tannins; trees
Abstract:
... Chestnut blight fungus, Cryphonectria parasitica, and its hypovirus present a useful model system for investigating the mechanisms of hypoviral infection. To identify gene products associated with fungal pathogenicity and hypoviral regulation, we attempted a proteomic analysis of the virus-free EP155/2 strain and its isogenic virus-infected UEP1 strain in response to tannic acid (TA), which is abu ...
DOI:
10.1139/w2012-065

78. Comparative vesicle proteomics reveals selective regulation of protein expression in chestnut blight fungus by a hypovirus

Author:
Jinzi Wang; Fangzhen Wang; Youjun Feng; Ke Mi; Qi Chen; Jinjie Shang; Baoshan Chen
Source:
Journal of proteomics 2013 v.78 pp. 221-230
ISSN:
1874-3919
Subject:
Cryphonectria parasitica; Hypovirus; energy metabolism; fungi; gene expression regulation; mass spectrometry; messenger RNA; molecular weight; pathogenesis; protein synthesis; proteins; proteomics; transcription (genetics); two-dimensional gel electrophoresis
Abstract:
... The chestnut blight fungus (Cryphonectria parasitica) and hypovirus constitute a model system to study fungal pathogenesis and mycovirus–host interaction. Knowledge in this field has been gained largely from investigations at gene transcription level so far. Here we report a systematic analysis of the vesicle proteins of the host fungus with/without hypovirus infection. Thirty-three differentially ...
DOI:
10.1016/j.jprot.2012.08.013
PubMed:
22954595

79. Comparison of the transcriptomes of American chestnut (Castanea dentata) and Chinese chestnut (Castanea mollissima) in response to the chestnut blight infection

Author:
Abdelali Barakat; Denis S DiLoreto; Yi Zhang; Chris Smith; Kathleen Baier; William A Powell; Nicholas Wheeler; Ron Sederoff; John E Carlson
Source:
BMC plant biology 2009 v.9 no.1 pp. 51
ISSN:
1471-2229
Subject:
Castanea dentata; Castanea mollissima; Cryphonectria parasitica; chestnut blight; computer simulation; ecological value; fungi; genomics; high-throughput nucleotide sequencing; pathogens; plant biology; stress response; transcriptome; unigenes
Abstract:
... BACKGROUND1471-2229-9-51: American chestnut (Castanea dentata) was devastated by an exotic pathogen in the beginning of the twentieth century. This chestnut blight is caused by Cryphonectria parasitica, a fungus that infects stem tissues and kills the trees by girdling them. Because of the great economic and ecological value of this species, significant efforts have been made over the century to c ...
DOI:
10.1186/1471-2229-9-51

80. Complete genome sequence of a novel mycovirus isolated from the phytopathogenic fungus Corynespora cassiicola in China

Author:
Yanfen Wang; Hang Zhao; Caiying Xue; Chao Xu; Yuehua Geng; Rui Zang; Yuxia Guo; Haiyan Wu; Meng Zhang
Source:
Archives of virology 2020 v.165 no.10 pp. 2401-2404
ISSN:
0304-8608
Subject:
Corynespora cassiicola; Cryphonectria parasitica; RNA-directed RNA polymerase; amino acids; double-stranded RNA; genome; leaf abscission; molecular weight; mycoviruses; phylogeny; plant pathogenic fungi; rubber; virology; China
Abstract:
... A novel mycovirus, named “Corynespora cassiicola bipartite mycovirus 1” (CcBV1), was isolated from a phytopathogenic fungus, Corynespora cassiicola, the causal agent of rubber leaf fall disease. The nucleotide sequence of the complete genome of CcBV1, which consists of two double-stranded RNA (dsRNA) segments, was determined. The first dsRNA is 2,002 bp in length and contains a single open reading ...
DOI:
10.1007/s00705-020-04723-2

81. Complete nucleotide sequences of dsRNA2 and dsRNA7 detected in the phytopathogenic fungus Sclerotium hydrophilum and their close phylogenetic relationship to a group of unclassified viruses

Author:
Chunlan Wang; Juan Wu; Xiwu Zhu; Jishuan Chen
Source:
Virus genes 2016 v.52 no.6 pp. 823-827
ISSN:
0920-8569
Subject:
Cryphonectria parasitica; Curvularia; Lactarius rufus; Penicillium aurantiogriseum; RNA viruses; RNA-directed RNA polymerase; Sclerotium hydrophilum; double-stranded RNA; heat tolerance; molecular cloning; mycelium; mycoviruses; open reading frames; phylogeny; plant pathogenic fungi; proteins; sequence analysis; unassigned viruses; virus-like particles
Abstract:
... Ten dsRNA segments were extracted from Sclerotium hydrophilum isolate (HZ11). The isolation of virus-like particles contained 10 dsRNA segments with the same number and migration as those extracted directly from the fungal mycelia. Two of these dsRNA segments, dsRNA2 and dsRNA7, were cloned and sequenced. They were 2121 and 1953 bp, respectively. The dsRNA2 encodes a RNA-dependent RNA polymerase. ...
DOI:
10.1007/s11262-016-1375-1

82. Conserved and variable structural elements in the 5′ untranslated region of two hypoviruses from the filamentous fungus Cryphonectria parasitica

Author:
Rong Mu; Tammy A. Romero; Kathryn A. Hanley; Angus L. Dawe
Source:
Virus research 2011 v.161 no.2 pp. 203-208
ISSN:
0168-1702
Subject:
Cryphonectria parasitica; Hypoviridae; RNA; computer software; digestion; fungi; genome; models; molecular biology; mycoviruses; prediction; ribonucleases; ribosomes; sequence analysis; translation (genetics); virulence
Abstract:
... Virulence-attenuating viruses (hypoviruses) of the filamentous fungus Cryphonectria parasitica, the causative agent of chestnut blight, have become a premier model for understanding the molecular biology of mycoviruses. However, a major gap exists in current understanding of structure and function of the untranslated regions (UTRs) of the hypovirus RNA genome, despite considerable evidence that se ...
DOI:
10.1016/j.virusres.2011.07.023
PubMed:
21884737
PubMed Central:
PMC3837689

83. Control of chestnut blight by the use of hypovirulent strains of the fungus Cryphonectria parasitica in northwestern Spain

Author:
P. Zamora; A.B. Martín; R. San Martín; P. Martínez-Álvarez; J.J. Diez
Source:
Biological control 2014 v.79 pp. 58-66
ISSN:
1049-9644
Subject:
Castanea; Cryphonectria parasitica; Hypovirus; biological control; field experimentation; fungi; orchards; stems; trees; virulence; viruses; Spain
Abstract:
... Chestnut blight is controlled in Europe by using Cryphonectria hypovirus CHV1, a non-encapsulated RNA virus. The chestnut blight fungus, Cryphonectria parasitica, is weakened by the virus, and healing tissue growth occurs in the host tree. Transmission of this cytoplasmic hypovirus is restricted by the incompatibility system of the fungus, so that the hypovirus can be transmitted only between isol ...
DOI:
10.1016/j.biocontrol.2014.08.005

84. Controlled Gene Expression in the Plant Pathogen Cryphonectria parasitica by Use of a Copper-Responsive Element

Author:
Willyerd, Karyn L.; Kemp, Amanda M.; Dawe, Angus L.
Source:
Applied and environmental microbiology 2009 v.75 no.16 pp. 5417-5420
ISSN:
0099-2240
Subject:
Castanea; Cryphonectria parasitica; gene expression; genes; plant pathogens; promoter regions; transporters
Abstract:
... We have developed a tool for controlled expression of heterologous or ectopic genes in the chestnut pathogen Cryphonectria parasitica using the promoter region from a putative copper-regulated transporter gene. In addition, we have found that expression control via this system is not affected by the virulence-attenuating hypovirus CHV1-EP713. ...
DOI:
10.1128/AEM.00899-09
PubMed:
19542340
PubMed Central:
PMC2725480

85. Correction to: A multiplexed genotyping assay to determine vegetative incompatibility and mating type in Cryphonectria parasitica

Author:
C. Cornejo; B. Šever; Q. Kupper; S. Prospero; D. Rigling
Source:
European journal of plant pathology 2019 v.155 no.1 pp. 377-379
ISSN:
0929-1873
Subject:
Cryphonectria parasitica; biological control; genotyping; mycoviruses
Abstract:
... Vendor overlooked Fig. 2 & 3 during proof correction and need to be replaced with these. ...
DOI:
10.1007/s10658-019-01773-4

86. Coupled termination/reinitiation for translation of the downstream open reading frame B of the prototypic hypovirus CHV1-EP713

Author:
Guo, Li-hua; Sun, Liying; Chiba, Sotaro; Araki, Hiroyuki; Suzuki, Nobuhiro
Source:
Nucleic acids research 2009 v.37 no.11 pp. 3645-3659
ISSN:
0305-1048
Subject:
stop codon; messenger RNA; translation (genetics); open reading frames; luciferase; virulence; ribosomes; viruses; mutants; Cryphonectria parasitica; insects; virus replication; Cryphonectria hypovirus 1; fungi
Abstract:
... Cryphonectria hypovirus 1 (CHV1), associated with the picorna-like superfamily, infects the chestnut blight fungus and attenuates the virulence of the host fungus. The genomic RNA of the virus has two continuous open reading frames, A and B, separated by the pentanucleotide UAAUG. We present here evidence suggesting that ORF B is translated from genome-sized virus mRNA by a coupled termination/rei ...
DOI:
10.1093/nar/gkp224
PubMed:
19364811
PubMed Central:
PMC2699510

87. CpBir1 is required for conidiation, virulence and anti-apoptotic effects and influences hypovirus transmission in Cryphonectria parasitica

Author:
Kun Gao; Qin Xiong; Jing Xu; Kuijie Wang; Kerong Wang
Source:
Fungal genetics and biology 2013 v.51 pp. 60-71
ISSN:
1087-1845
Subject:
Animalia; Cryphonectria parasitica; Hypovirus; Saccharomyces cerevisiae; apoptosis; conidiation; cytoplasm; gene overexpression; genes; genetic complementation; hydrogen peroxide; hyphae; mutants; mycelium; phenotype; pigmentation; proteins; stress tolerance; virulence; viruses; yeasts
Abstract:
... Inhibitors of apoptosis proteins (IAPs) are critically important in the regulation of unicellular yeast and metazoan apoptosis. All IAPs contain one to three baculovirus IAP repeat (BIR) domains, which are essential for the anti-apoptotic activity of the IAPs. A homolog of IAPs, CpBir1, which bears two BIR domains, was recently identified from the chestnut blight fungus Cryphonectria parasitica ge ...
DOI:
10.1016/j.fgb.2012.09.011
PubMed:
23084963

88. Cpkk1, MAPKK of Cryphonectria parasitica, Is Necessary for Virulence on Chestnut

Author:
Rostagno, Laura; Prodi, Antonio; Turina, Massimo
Source:
Phytopathology 2010 v.100 no.10 pp. 1100-1110
ISSN:
0031-949X
Subject:
Castanea sativa; chestnuts; Cryphonectria parasitica; plant pathogenic fungi; fungal diseases of plants; tree diseases; virulence; microbial genetics; mitogen-activated protein kinase; mutants; promoter regions; alleles; messenger RNA; gene silencing; sporulation; conidia; defense mechanisms
Abstract:
... The role of Cpkk1, a mitogen-activated protein kinase from Cryphonectria parasitica, was investigated by generating a number of mutant strains that overexpress, under the control of the cryparin promoter, both the wild-type protein and its allele with an extensive deletion in the catalytic domain. Furthermore, a hairpin construct was built and expressed to cause specific silencing of Cpkk1 mRNA tr ...
DOI:
10.1094/PHYTO-02-10-0044
PubMed:
20839945

89. Cpvma1, a Vacuolar H⁺-ATPase Catalytic Subunit of Cryphonectria parasitica, is Essential for Virulence and Hypovirus RNA Accumulation

Author:
Ru Li; Shan Bai; Yuanyuan He; Qi Chen; Yanping Yao; Jinzi Wang; Baoshan Chen
Source:
Phytopathology 2019 v.109 no.8 pp. 1417-1424
ISSN:
0031-949X
Subject:
Castanea; Cryphonectria parasitica; H-transporting ATP synthase; H-transporting ATPase; Hypovirus; RNA; calcium; enzyme activity; eukaryotic cells; homeostasis; mutants; pH; phenotype; protein subunits; proton pump; sporulation; vacuoles; virulence
Abstract:
... The vacuolar H⁺-ATPases (V-ATPases) are conserved ATP-dependent proton pumps that acidify intracellular compartments in eukaryotic cells. The role of Cpvma1, a V-ATPase catalytic subunit A of Cryphonectria parasitica, was investigated by generating cpvma1-overexpressing and cpvma1-silenced strains. The mutant strains were evaluated for phenotypic characteristics, V-ATPase activity, response to ele ...
DOI:
10.1094/PHYTO-08-18-0289-R

90. Cryphonectria hypovirus 1 (CHV‐1) survey reveals low occurrence and diversity of subtypes in NE Spain

Author:
C. Castaño; L. Bassie; D. Oliach; M. Gómez; V. Medina; B. Liu; C. Colinas; Y. Balci
Source:
Forest pathology 2015 v.45 no.1 pp. 51-59
ISSN:
1437-4781
Subject:
Castanea sativa; Cryphonectria hypovirus 1; Cryphonectria parasitica; biological control; fungi; haplotypes; nucleotide sequences; phylogeny; sequence analysis; surveys; Spain
Abstract:
... Cryphonectria parasitica (Murrill.) Barr is a necrotrophic fungus that affects European chestnut populations. In Catalonia (NE Spain), this fungus is widely spread affecting chestnut stands. In addition to vegetative compatibility (VC) types of the fungus, knowledge of the Cryphonectria hypovirus (CHV) occurrence is important to implement a biocontrol programme. We confirmed the presence of hypovi ...
DOI:
10.1111/efp.12131

91. Cryphonectria hypovirus 1-Induced Changes of Stress Enzyme Activity in Transfected Phytopathogenic Fungus Cryphonectria parasitica

Author:
Lucija Nuskern; Mirta Tkalec; Marin Ježić; Zorana Katanić; Ljiljana Krstin; Mirna Ćurković-Perica
Source:
Microbial ecology 2017 v.74 no.2 pp. 302-311
ISSN:
0095-3628
Subject:
Castanea; Cryphonectria hypovirus 1; Cryphonectria parasitica; catalase; enzyme activity; genetic variation; glutathione transferase; messenger RNA; microbial growth; mycoviruses; oxidative stress; plant pathogenic fungi; proteins; stress response; superoxide dismutase; transcriptomics; trees; virulence; East Asia; Europe; North America
Abstract:
... Cryphonectria parasitica is a phytopathogenic fungus introduced from Eastern Asia to North America and to Europe, where it causes chestnut blight, a devastating disease of chestnut trees. The disease can be biologically controlled utilising the mycovirus Cryphonectria hypovirus 1 (CHV1), which changes the physiology of the host, reducing its virulence towards chestnut. We measured fungal growth in ...
DOI:
10.1007/s00248-017-0945-7

92. Cryphonectria hypovirus 1-Induced Epigenetic Changes in Infected Phytopathogenic Fungus Cryphonectria parasitica

Author:
Lucija Nuskern; Marin Ježić; Zlatko Liber; Jelena Mlinarec; Mirna Ćurković-Perica
Source:
Microbial ecology 2018 v.75 no.3 pp. 790-798
ISSN:
0095-3628
Subject:
Castanea; Cryphonectria hypovirus 1; Cryphonectria parasitica; DNA methylation; animals; biological control; biotic stress; enzymes; epigenetics; forest ecosystems; genome; microbial growth; models; mycoviruses; orchards; plant pathogenic fungi; plants (botany); virulence
Abstract:
... Biotic stress caused by virus infections induces epigenetic changes in infected plants and animals, but this is the first report on methylation pattern changes in a fungus after mycovirus infection. As a model pathosystem for mycovirus-host interactions, we used Cryphonectria hypovirus 1 (CHV1) and its host fungus Cryphonectria parasitica, in which deregulation of methylation cycle enzymes upon vi ...
DOI:
10.1007/s00248-017-1064-1

93. Cryphonectria naterciae: A new species in the Cryphonectria–Endothia complex and diagnostic molecular markers based on microsatellite-primed PCR

Author:
Helena Bragança; Daniel Rigling; Eugénio Diogo; Jorge Capelo; Alan Phillips; Rogério Tenreiro
Source:
Fungal biology 2011 v.115 no.9 pp. 852-861
ISSN:
1878-6146
Subject:
Castanea sativa; Cryphonectria parasitica; Quercus suber; decline; genetic markers; internal transcribed spacers; new species; polymerase chain reaction; tubulin
Abstract:
... In a recent study intended to assess the distribution of Cryphonectria parasitica in Portugal, 22 morphologically atypical orange isolates were collected in the Midwestern regions. Eleven isolates were recovered from Castanea sativa, in areas severely affected by chestnut blight and eleven isolates from Quercus suber in areas with cork oak decline. These isolates were compared with known C. parasi ...
DOI:
10.1016/j.funbio.2011.06.014
PubMed:
21872182

94. Cryphonectria parasitica in Portugal: diversity of vegetative compatibility types, mating types, and occurrence of hypovirulence

Author:
Bragança, H.; Simões, S.; Onofre, N.; Tenreiro, R.; Rigling, D.
Source:
Forest pathology 2007 v.37 no.6 pp. 391-402
ISSN:
1437-4781
Subject:
Castanea; Cryphonectria parasitica; double-stranded RNA; fungi; sexual reproduction; virulence; Azores; Portugal
Abstract:
... Cryphonectria parasitica, the causal agent of chestnut blight, has been responsible for the decline in chestnut in Portugal for the last two decades. In order to improve understanding of C. parasitica diversity, a total of 617 isolates from all affected chestnut-growing areas in continental Portugal, Madeira and the Azores archipelagos were studied. Nine vegetative compatibility (vc) types were id ...
DOI:
10.1111/j.1439-0329.2007.00513.x

95. Cryphonectria parasitica isolates of the same vegetative compatibility type display different rates of transfer of CHV1 hypovirus

Author:
P. Zamora; A. B. Martín; M. Dueñas; R. San Martin; J. J. Diez
Source:
European journal of plant pathology 2015 v.143 no.4 pp. 767-777
ISSN:
0929-1873
Subject:
Castanea; Cryphonectria parasitica; Hypovirus; assays; fungi; genotype; strains; virulence; Spain
Abstract:
... Hypovirulent strains of the chestnut blight fungus Cryphonectria parasitica have not been registered in the autonomous region of Castilla y León (Spain), except in the province of León. In this laboratory-based study, we analyzed the rates of horizontal transmission of hypovirus CHV1 subtype F1, isolated from chestnut stands in León. We tested the conversion capacity of the six vegetative compatib ...
DOI:
10.1007/s10658-015-0727-3

96. Cryphonectria parasitica vegetative compatibility type analysis of populations in south-western France and northern Spain

Author:
Robin, C.; Capdevielle, X.; Martin, M.; Traver, C.; Colinas, C.
Source:
Plant pathology 2009 v.58 no.3 pp. 527-535
ISSN:
0032-0862
Subject:
Castanea; Cryphonectria parasitica; biological control; forests; genes; genetics; mating types; mountains; orchards; sexual reproduction; France; Spain
Abstract:
... A comprehensive study of the population biology of Cryphonectria parasitica, the causal agent of chestnut blight, is required to understand the spread of the epidemic in Europe and its natural regulation by the Cryphonectria hypovirus-1 (CHV-1). With this objective in mind, the diversity in vegetative compatibility (vc) types and mating types of C. parasitica populations was assessed in 43 chestnu ...
DOI:
10.1111/j.1365-3059.2008.01986.x

97. Cryphonectria parasitica, the causal agent of chestnut blight: invasion history, population biology and disease control

Author:
Daniel Rigling; Simone Prospero
Source:
Molecular plant pathology 2018 v.19 no.1 pp. 7-20
ISSN:
1464-6722
Subject:
Acer; Carpinus betulus; Castanea crenata; Castanea dentata; Castanea mollissima; Castanea pumila; Castanea sativa; Cryphonectria hypovirus 1; Cryphonectria parasitica; Quercus; backcrossing; bark; biological control; biological control agents; blight; branches; burning; cutting; disease control; fruiting bodies; fungi; genome; host range; hosts; mycelium; mycoviruses; pathogens; shoots; sporulation; taxonomy; trees; virulence; wilting; East Asia; Europe; North America
Abstract:
... Chestnut blight, caused by Cryphonectria parasitica, is a devastating disease infecting American and European chestnut trees. The pathogen is native to East Asia and was spread to other continents via infected chestnut plants. This review summarizes the current state of research on this pathogen with a special emphasis on its interaction with a hyperparasitic mycovirus that acts as a biological co ...
DOI:
10.1111/mpp.12542
PubMed:
28142223
PubMed Central:
PMC6638123

98. Cryphonectria parasitica, the chestnut blight fungus, causes cankers on Quercus frainetto in Greece

Author:
George T. Tziros; Zacharoula G. Nakopoulou; Stephanos Diamandis
Source:
Australasian plant disease notes 2015 v.10 no.1 pp. 19
ISSN:
1833-928X
Subject:
Cryphonectria parasitica; Quercus frainetto; branches; fungi; internal transcribed spacers; pathogenicity; pathogens; sequence analysis; tubulin; Greece
Abstract:
... In 2011, cankers were observed on Hungarian oak trees (Quercus frainetto) in three localities in Greece. The pathogen was identified as Cryphonectria parasitica on the basis of morphology and sequence analysis of the internal transcribed region (ITS), part of the elongation factor 1a (EF-1a) and part of the beta-tubulin region (βT). A pathogenicity test was performed and Koch’s postulates were con ...
DOI:
10.1007/s13314-015-0171-4

99. Cryphonectric acid and other minor metabolites from a hypovirulent strain of Cryphonectria parasitica

Author:
Arnone, A.; Assante, G.; Nasini, G.; Strada, S.; Vercesi, A.
Source:
Journal of natural products 2002 v.65 no.1 pp. 48-50
ISSN:
0163-3864
Subject:
Castanea dentata; Zea mays; roots; seedlings; strain differences; bioassays; secondary metabolites; plant pathogenic fungi; growth retardation; spectral analysis; chemical constituents of plants; Castanea sativa; Cryphonectria parasitica; virulence; chemical structure; Solanum lycopersicum var. lycopersicum
DOI:
10.1021/np0103012
PubMed:
11809063

100. Crypt1, an active Ac-like transposon from the chestnut blight fungus, Cryphonectria parasitica

Author:
D. Linder-Basso; R. Foglia; P. Zhu; B.I. Hillman
Source:
Molecular genetics and genomics 2001 v.265 no.4 pp. 730-738
ISSN:
1617-4615
Subject:
proteins; messenger RNA; open reading frames; amino acid sequences; genomics; Cryphonectria parasitica; transposons
Abstract:
... A moderately repetitive element was identified previously in the nuclear genome of the chestnut blight fungus, Cryphonectria parasitica, and has been used as a probe for population studies of the fungus. We report here that the repetitive element is a class II transposon of the hAT family of Activator (Ac)-like transposable elements. The element, named Crypt1, has a size of 3563 bp, including 21-b ...
DOI:
10.1007/s004380100470
PubMed:
11459194