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- Zhang, Weican, et al. Show all 5 Authors
- Applied microbiology and biotechnology 2017 v.101 no.5 pp. 1919-1926
- Cytophaga hutchinsonii; Escherichia coli; Gram-negative bacteria; beta-glucosidase; bioenergy industry; cellobiose; cellulose; cellulosome; genomics; glucose; substrate specificity
- ... Cytophaga hutchinsonii is a gram-negative bacterium that can efficiently degrade crystalline cellulose by a novel strategy without cell-free cellulases or cellulosomes. Genomic analysis implied that C. hutchinsonii had endoglucanases and β-glucosidases but no exoglucanases which could processively digest cellulose and produce cellobiose. In this study, BglA was functionally expressed in Escherichi ...
- Zhang, Weican, et al. Show all 3 Authors
- Applied microbiology and biotechnology 2015 v.99 no.22 pp. 9617-9623
- Gram-negative bacteria; Cytophaga hutchinsonii; calcium; carboxymethylcellulose; denaturation; active sites; carbohydrate binding; genomics; temperature; endo-1,4-beta-glucanase; glycosides; Escherichia coli
- ... Cytophaga hutchinsonii is a Gram-negative bacterium that can degrade crystalline cellulose efficiently with an unknown strategy. Genomic analysis suggested it lacks exoglucanases which are found in many cellulolytic organisms and most of the cellulases in C. hutchinsonii lack recognizable carbohydrate-binding modules (CBMs). CHU_1280, speculated to be an endoglucanase belonging to glycoside hydrol ...