Subject term: DNA and Florida - PubAg Search Results

1. Contextualizing enigmatic extinctions using genomic DNA from fluid-preserved museum specimens of Desmognathus salamanders

Author:: R. Alexander Pyron; David A. Beamer; Chace R. Holzheuser; Emily Moriarty Lemmon; Alan R. Lemmon; Addison H. Wynn; Kyle A. O’Connell
Source:: Conservation genetics 2022 v.23 no.2 pp. 375-386
ISSN:: 1566-0621
Subject:: DNA; DNA damage; Desmognathus; conservation genetics; conspecificity; extinction; formalin; genomics; hybrids; mitochondria; museums; natural history; phylogeny; salamanders and newts; spring; Florida
Abstract:: ... Species that went extinct prior to the genomic era are often considered out-of-reach for modern phylogenetic studies. This is particularly limiting for conservation studies, as genetic data from such taxa may be key to understanding extinction risks and causes of decline that can inform the management of related, extant populations. Fortunately, continual advances in biochemistry and DNA sequencin ...
DOI:: 10.1007/s10592-021-01424-4
: https://doi.org/10.1007/s10592-021-01424-4

2. First Report of Botrytis cinerea Causing Leaf Spot on Strawberry in Florida

Author:: M. V. Marin; N. A. Peres
Source:: Plant disease 2022 v.106 no.4 pp. 1298
ISSN:: 0191-2917
Subject:: Botrytis cinerea; DNA; DNA-directed RNA polymerase; Fragaria ananassa; coasts; conidia; cultivars; disease incidence; education; fruits; fungi; fungicide resistance; glyceraldehyde-3-phosphate dehydrogenase; gray mold; greenhouses; heat shock proteins; leaf blight; leaf spot; leaves; mycelium; pathogenicity; pathogens; plant rots; polysorbates; runoff; sclerotia; sensation; sporulation; strawberries; California; Florida
Abstract:: ... During the 2020–21 Florida strawberry season (October to April), strawberry (Fragaria × ananassa) plants showing leaf spots were observed on samples submitted to the Diagnostic Clinic at the University of Florida’s Gulf Coast Research and Education Center. Disease incidence was up to 5% and observed on four different farms in Plant City, FL on cultivars Sensation Florida127 and Florida Brilliance. ...
DOI:: 10.1094/PDIS-05-21-1082-PDN
: https://doi.org/10.1094/PDIS-05-21-1082-PDN

3. First Report of Meloidogyne javanica Pathogenic on Hybrid Lavender (Lavandula × intermedia) in the United States

Author:: S. A. Oliveira; D. M. Dlugos; P. Agudelo; S. N. Jeffers
Source:: Plant disease 2022 v.106 no.1 pp. 335
ISSN:: 0191-2917
Subject:: DNA; Meloidogyne javanica; Phytophthora; crown rot; eggs; geographical distribution; greenhouses; host range; hybrids; pastures; pathogenicity; root-knot nematodes; sandy loam soils; soilless media; species identification; summer; tomatoes; Brazil; Florida; Kentucky; South Carolina
Abstract:: ... Root-knot nematodes (RKNs), Meloidogyne spp., are some of the most economically important pathogens of cultivated plants. Meloidogyne javanica is one of the most destructive RKN species and is well known for its broad host range and the severe damage it causes to plant roots (Perry et al. 2009). In February 2018, four mature dead and dying hybrid lavender plants (Lavandula × intermedia ‘Phenomenal ...
DOI:: 10.1094/PDIS-06-21-1175-PDN
: https://doi.org/10.1094/PDIS-06-21-1175-PDN

4. First Report of Neopestalotiopsis clavispora Causing Calyx and Receptacle Blight on Strawberry in China

Author:: Jiajun Shi; Ximin Zhang; Ye Liu; Zhihong Zhang; Zhigang Wang; Chunsheng Xue; Yue Ma; Feng Wang
Source:: Plant disease 2022 v.106 no.4 pp. 1307
ISSN:: 0191-2917
Subject:: DNA; Fragaria ananassa; Neopestalotiopsis clavispora; blight; calyx; color; conidia; fungi; genes; greenhouses; internal transcribed spacers; leaf spot; mycelium; pathogenicity; pathogens; phylogeny; relative humidity; root rot; sequence analysis; strawberries; Argentina; China; Florida; Spain
Abstract:: ... Strawberry (Fragaria × ananassa) is widely cultivated in Liaoning Province, the most important strawberry production area in China. In November 2020, extensive brown spotting on calyces and receptacles and dark brown necrotic lesions on leaves of ‘Yanli’ strawberry plants were observed in the greenhouse, College of Horticulture, in Shenyang Agriculture University of Liaoning Province, China. The i ...
DOI:: 10.1094/PDIS-07-21-1376-PDN
: https://doi.org/10.1094/PDIS-07-21-1376-PDN

5. Genome-wide assessment of population structure in Florida’s coastal seaside sparrows

Author:: Carolyn Enloe; W. Andrew Cox; Akanksha Pandey; Sabrina S. Taylor; Stefan Woltmann; Rebecca T. Kimball
Source:: Conservation genetics 2022 v.23 no.2 pp. 285-297
ISSN:: 1566-0621
Subject:: DNA; Passeriformes; coasts; conservation genetics; genetic relationships; population structure; Florida
Abstract:: ... The distribution and genetic relatedness of subspecies among the populations of seaside sparrow (Ammospiza maritima [Wilson 1810]) along the coast of Florida are poorly understood. We evaluated genetic support for three defined Florida Gulf coast subspecies, as well as a fourth subspecies on the Atlantic coast, and assessed whether genetic clusters corresponded geographically with the defined rang ...
DOI:: 10.1007/s10592-021-01411-9
: https://doi.org/10.1007/s10592-021-01411-9

6. Review of the genus Palmicultor Williams, 1963 (Hemiptera: Coccomorpha: Pseudococcidae), with evidence of a cryptic species

Author:: Natalia von Ellenrieder; Scott A. Kinnee; Gillian W. Watson
Source:: Pan-Pacific entomologist 2022 v.97 no.4 pp. 240-260
ISSN:: 0031-0603
Subject:: adults; cryptic species; entomologists; invasive species; mitochondria; taxonomic keys; California; Florida
Abstract:: ... In this study, molecular and morphological analysis of Palmicultor Williams, 1963 (Hemiptera: Coccomorpha: Pseudococcidae) mealybugs, intercepted on plant material entering California and collected in Florida, provides evidence for the existence of a cryptic species under the taxon currently known as P. palmarum (Ehrhorn, 1916). A characterization and analysis based on three molecular markers (mit ...
DOI:: 10.3956/2021-97.4.240
: https://doi.org/10.3956/2021-97.4.240

7. Absence of Herpesvirus in a Survey of Alligator Snapping Turtles (Macrochelys temminckii) in Illinois and Louisiana

Author:: Taylor J. Willis; Laura Adamovicz; Ethan Kessler; Peter M. DiGeronimo; Matthew C. Allender
Source:: Journal of herpetological medicine and surgery 2021 v.31 no.1 pp. 43-47
ISSN:: 1529-9651
Subject:: DNA; Herpesviridae infections; Macrochelys temminckii; adults; alligators; cloaca; medicine; monitoring; necrosis; polymerase chain reaction; surgery; surveys; Florida; Illinois; Louisiana
Abstract:: ... Herpesviruses are significant pathogens of captive-reared and free-ranging chelonians worldwide. Lesions associated with chelonian herpesvirus infection are species dependent and include stomatitis in tortoises, hepatic necrosis in pond turtles, and fibropapillomas in sea turtles, among other conditions. Herpesviruses also have been detected in several free-ranging freshwater turtle species with n ...
DOI:: 10.5818/19-09-210.3
: https://doi.org/10.5818/19-09-210.3

8. Caulerpa wysorii sp. nov., a denuded Caulerpa (Chlorophyta) resembling C. sertularioides when ‘dressed’

Author:: Thomas Sauvage; Michael J. Wynne; Stefano G.A. Draisma; Ileana Ortegón-Aznar; Luz Elena Mateo-Cid; A. Catalina Mendoza-González; Beatriz Martínez-Daranas; Suzanne Fredericq
Source:: Phycologia 2021 v.60 no.2 pp. 107-119
ISSN:: 2330-2968
Subject:: Caulerpa; DNA; DNA barcoding; chloroplast genes; conspecificity; lectotypes; morphs; new species; Florida
Abstract:: ... Routine DNA barcoding with the chloroplast gene tufA identified novel records of an unknown and denuded (i.e. lacking branchlets) Caulerpa species previously reported from the Florida Middle Grounds. tufA barcoding not only confirmed the matching molecular identity of this taxon with several newly collected denuded specimens from shallow habitats of the Yucatán shelf, but also with a set of plumos ...
DOI:: 10.1080/00318884.2020.1862563
: https://doi.org/10.1080/00318884.2020.1862563

9. Development of a Multiplex High-Throughput Diagnostic Assay for the Detection of Strawberry Crown Rot Diseases Using High-Resolution Melting Analysis

Author:: Nan-Yi Wang; Andre Bueno Gama; Marcus Vinicius Marin; Natalia A. Peres
Source:: Phytopathology 2021 v.111 no.8 pp. 1470-1483
ISSN:: 0031-949X
Subject:: Colletotrichum; DNA; Macrophomina phaseolina; Phytophthora; chemical control; cost effectiveness; cross reaction; crown rot; disease control; disease diagnosis; pathogens; plant pathology; protocols; strawberries; Florida
Abstract:: ... Rapid and accurate disease diagnosis is a prerequisite for an effective disease management program in strawberry production. In Florida, Colletotrichum spp., Phytophthora spp., and Macrophomina phaseolina are the primary microorganisms causing strawberry crown rot. Even though the diseases can be caused by different pathogens, symptoms are indistinguishable and equally devastating. To inform straw ...
DOI:: 10.1094/PHYTO-12-20-0556-R
: https://doi.org/10.1094/PHYTO-12-20-0556-R

10. Development of a simple one-step multiplex RT-PCR system for simultaneous detection of DNA and RNA viruses of cucurbit leaf crumple virus, cucurbit yellow stunting disorder virus, squash vein yellowing virus, and cucurbit chlorotic yellows virus

Author:: A. Abdul Kader Jailani; Katherine Hendricks; Pamela D. Roberts; Mathews L. Paret
Source:: Physiological and molecular plant pathology 2021 v.116 pp. 101734
ISSN:: 0885-5765
Subject:: Cucurbit leaf crumple virus; Cucurbitaceae; DNA; RNA; cost effectiveness; genes; plant pathology; ribulose-bisphosphate carboxylase; squashes; viruses; watermelons; Florida
Abstract:: ... A highly sensitive one-step multiplex RT-PCR (OMRT-PCR) system with an internal plant gene control was developed to simultaneously detect single and mixed RNA and DNA viral pathogens affecting watermelon and squash in the Southeastern United States. Current methods rely on a singleplex detection system for several viruses which is time-consuming for large-scale field monitoring. The gene-specific ...
DOI:: 10.1016/j.pmpp.2021.101734
CHORUS:: 10.1016/j.pmpp.2021.101734
: https://doi.org/10.1016/j.pmpp.2021.101734

11. Esteya floridanum sp. nov.: An Ophiostomatalean Nematophagous Fungus and Its Potential to Control the Pine Wood Nematode

Author:: You Li; Haiying Yu; João P. M. Araújo; Xinfeng Zhang; Yingchao Ji; Jiri Hulcr
Source:: Phytopathology 2021 v.111 no.2 pp. 304-311
ISSN:: 0031-949X
Subject:: Bursaphelenchus xylophilus; DNA; Larix gmelinii var. olgensis; Pinus koraiensis; biological control; conifers; death; natural enemies; nematophagous fungi; new species; pests; phylogeny; plant pathogens; plant pathology; surveys; vascular wilt; China; Europe; Florida
Abstract:: ... The nematophagous fungal genus Esteya is reported as a natural enemy of the pine wood nematode Bursaphelenchus xylophilus, which causes pine wilt in Asia and Europe. During a survey of fungi associated with ambrosia beetles in Florida, an undescribed Esteya species was found. A phylogenetic analysis based on nuclear large subunit and β-tubulin DNA sequences supported this isolate as a new species, ...
DOI:: 10.1094/PHYTO-06-20-0229-R
: https://doi.org/10.1094/PHYTO-06-20-0229-R

12. First Report of Alternaria spp. Causing Leaf Spot on Sweet Viburnum in China

Author:: Chaodong Qiu; Yingying Zhang; Zhenyu Liu
Source:: Plant disease 2021 v.105 no.8 pp. 2253
ISSN:: 0191-2917
Subject:: Alternaria; DNA; Viburnum odoratissimum; actin; adenosinetriphosphatase; air temperature; allergens; ampicillin; beak; calmodulin; conidia; conidiophores; cotton; culture media; data collection; disinfection; ethanol; foliar diseases; fungi; kanamycin; leaf spot; leaves; mycelium; pathogenicity; pathogens; phylogeny; plasma membrane; shrubs; statistical analysis; China; Florida
Abstract:: ... Sweet viburnum (Viburnum odoratissimum [L.] Ker Gawl) is an evergreen shrub mainly cultivated along roadsides in urban landscapes and in parks and residential areas. A foliar disease occurred on ∼40% of sweet viburnum plants near Anhui Grand Theater, Anhui Province, China, in June 2019. Early symptoms appeared as small brown spots 2 to 3 mm in length on the leaves. Spots developed on the upper, mi ...
DOI:: 10.1094/PDIS-06-20-1407-PDN
: https://doi.org/10.1094/PDIS-06-20-1407-PDN

13. First Report of Bipolaris yamadae Leaf Spot Disease on Guinea Grass (Panicum maximum) in Florida

Author:: A. Adhikari; X. Wang; B. Lane; P. F. Harmon; E. M. Goss
Source:: Plant disease 2021 v.105 no.4 pp. 1208
ISSN:: 0191-2917
Subject:: Bayesian theory; Bipolaris; DNA; Hawaii; Japan; Megathyrsus maximus; Panicum; agar; canopy; color; conidia; conidiophores; corn; culture media; gardens; genes; glyceraldehyde-3-phosphate dehydrogenase; grasses; internal transcribed spacers; leaf blight; leaf spot; models; mycelium; phylogeny; runoff; sprayers; statistical analysis; sugarcane; weeds; China; Cuba; Florida; India; Louisiana; Texas
Abstract:: ... Guinea grass is an invasive perennial C4 grass and is a common weed around agricultural crops in Louisiana, Texas, and Hawaii, U.S.A. (Overholt and Franck 2017). In November 2018, leaf spots were observed on Guinea grass occurring in an organic garden located in Gainesville, Florida, U.S.A. Lesions were oblong to irregular, dark gray to brownish center with pale-yellow to brownish black margin. Le ...
DOI:: 10.1094/PDIS-07-20-1486-PDN
: https://doi.org/10.1094/PDIS-07-20-1486-PDN

14. First Report of Diaporthe phaseolorum Causing Stem Canker of Hemp (Cannabis sativa)

Author:: M. V. Marin; N.-Y. Wang; J. D. Coburn; J. Desaeger; N. A. Peres
Source:: Plant disease 2021 v.105 no.7 pp. 2018
ISSN:: 0191-2917
Subject:: Bayesian theory; Cannabis sativa; DNA; Diaporthe phaseolorum; Helianthus annuus; Phaseolus; anamorphs; calmodulin; conidia; culture media; disinfestation; field experimentation; fungi; greenhouses; growth chambers; hemp; herbaceous plants; internal transcribed spacers; mycelium; oils; pathogenicity; pathogens; perithecia; phylogeny; polysorbates; pycnidia; specialty crops; statistical analysis; stem cankers; syringes; Florida
Abstract:: ... Hemp is an annual herbaceous plant that is used for its fiber and oil in a variety of commercial and industrial products. In Florida, it is currently being explored as a new specialty crop. During a field trial from October to January 2019 in Wimauma, FL, a stem canker was observed on up to 60% of 3-month-old plants of ‘Eletta Campana’, ‘Carmagnola Selezionata’, and ‘Tygra’. Symptoms started on th ...
DOI:: 10.1094/PDIS-06-20-1174-PDN
: https://doi.org/10.1094/PDIS-06-20-1174-PDN

15. First Report of Neopestalotiopsis rosae Causing Foliar and Fruit Spots on Pomegranate in Florida

Author:: K. V. Xavier; X. Yu; G. E. Vallad
Source:: Plant disease 2021 v.105 no.2 pp. 504
ISSN:: 0191-2917
Subject:: DNA; Neopestalotiopsis; Punica granatum; conidia; cryptic species; cultivars; defoliation; disease severity; ethanol; fruits; fungal growth; growth chambers; humidity; leaves; mycelium; pathogens; phylogeny; pomegranates; pycnidia; sand; statistical analysis; surveys; tissue paper; Florida; New Zealand
Abstract:: ... Foliar and fruit spots were observed on pomegranate trees (Punica granatum L.) in orchards at Balm and Zolfo Springs, FL, in 2019. Symptoms on leaves and fruits included dark brown to black irregular spots, 0.2 to 1.5 cm, with gray centers. Visible pycnidia were in the center of the lesions. Leaves became chlorotic and prematurely dropped. In a survey of 24 pomegranate cultivars, all trees were in ...
DOI:: 10.1094/PDIS-06-20-1282-PDN
: https://doi.org/10.1094/PDIS-06-20-1282-PDN

16. First Report of Neoscytalidium dimidiatum Causing Stem Canker on Dragon fruit (Hylocereus spp.) in Puerto Rico

Author:: L. M. Serrato-Diaz; R. Goenaga
Source:: Plant disease 2021 v.105 no.9 pp. 2728
ISSN:: 0191-2917
Subject:: DNA; Hylocereus; Neoscytalidium dimidiatum; cladodes; conidia; culture media; ethanol; fruits; internal transcribed spacers; mycelium; pathogenicity; peptide elongation factors; pycnidia; stem cankers; streptomycin; surveys; California; China; Florida; Israel; Malaysia; Puerto Rico; South America; Taiwan
Abstract:: ... Dragon fruit or pitahaya (Hylocereus spp.) is a tropical fruit belonging to the Cactaceae. It is native to Central and South America and commercially grown in the United States in southern California, south Florida, and Puerto Rico. During a disease survey from April to June 2020, stem canker was observed in greenhouses and commercial orchards located in Mayagüez and San Sebastian, Puerto Rico, wi ...
Handle:: 10113/7594326
DOI:: 10.1094/PDIS-10-20-2265-PDN
: https://doi.org/10.1094/PDIS-10-20-2265-PDN

17. Incidence of Asiatic Citrus Canker on Trifoliate Orange and Its Hybrid Accessions in a Florida Field Planting

Author:: Ed Stover; Chandrika Ramadugu; Mikeal Roose; Joseph Krystel; Richard F. Lee; Manjunath Keremane
Source:: HortScience 2021 v.56 no.5 pp. 525-531
ISSN:: 2327-9834
Subject:: Citrus; DNA; Poncirus trifoliata; USDA; Xanthomonas citri subsp. citri; chloroplasts; citrus canker; genotype; grapefruits; hybrids; oranges; planting; progeny; quantitative polymerase chain reaction; California; Florida
Abstract:: ... Asiatic citrus canker (ACC) foliar lesions were evaluated on progenies of 84 seed-source genotypes (“parent genotypes”) from the Citrus Variety Collection (CVC) of the University of California at Riverside (UCR) of Citrus trifoliata and hybrids between C. trifoliata and other Citrus species and hybrids. Trees were planted Aug. 2013 in a completely randomized design at the Fort Pierce U.S. Departme ...
DOI:: 10.21273/HORTSCI15684-20
: https://doi.org/10.21273/HORTSCI15684-20

18. Insights on Immune Function in Free-Ranging Green Sea Turtles (Chelonia mydas) with and without Fibropapillomatosis

Author:: Justin R. Perrault; Milton Levin; Cody R. Mott; Caitlin M. Bovery; Michael J. Bresette; Ryan M. Chabot; Christopher R. Gregory; Jeffrey R. Guertin; Sarah E. Hirsch; Branson W. Ritchie; Steven T. Weege; Ryan C. Welsh; Blair E. Witherington; Annie Page-Karjian
Source:: Animals 2021 v.11 no.3 pp. -
ISSN:: 2076-2615
Subject:: Alphaherpesvirinae; Chelonia mydas; DNA; T-lymphocytes; concanavalin A; immune response; immunosuppression; juveniles; lakes; lymphocyte proliferation; mitogens; natural killer cells; neoplasms; rivers; sea turtles; seroprevalence; Florida
Abstract:: ... Chelonid alphaherpesviruses 5 and 6 (ChHV5 and ChHV6) are viruses that affect wild sea turtle populations. ChHV5 is associated with the neoplastic disease fibropapillomatosis (FP), which affects green turtles (Chelonia mydas) in panzootic proportions. ChHV6 infection is associated with lung-eye-trachea disease (LETD), which has only been observed in maricultured sea turtles, although antibodies to ...
DOI:: 10.3390/ani11030861
: https://doi.org/10.3390/ani11030861

19. Phosphorus retention within a relic agricultural ditch in a constructed wetland

Author:: Bobby G. Duersch; Matthew O. Powers; Sue Newman; John G. Ricca; Jehangir H. Bhadha; J. William Louda
Source:: Journal of environmental quality 2021 v.50 no.5 pp. 1171-1183
ISSN:: 0047-2425
Subject:: DNA; agricultural runoff; constructed wetlands; environmental quality; marshes; nuclear magnetic resonance spectroscopy; nucleotides; orthophosphates; phosphorus; sediments; stormwater management; Florida
Abstract:: ... Stormwater treatment areas (STAs) are constructed wetlands established to capture phosphorus (P) from agricultural runoff before reaching the Florida Everglades. Retained P is primarily stored in wetland soils and sediments generated through a collection of interrelated physical, chemical, and biological processes. The amount of P and other elements (Al, Ca, Cu, Fe, Mg, Mn, Pb, and Zn) retained in ...
DOI:: 10.1002/jeq2.20278
: https://doi.org/10.1002/jeq2.20278

20. Reptile Host Associations of Ixodes scapularis in Florida and Implications for Borrelia spp. Ecology

Author:: Carrie De Jesus; Chanakya Bhosale; Kristen Wilson; Zoe White; Samantha M. Wisely
Source:: Pathogens 2021 v.10 no.8 pp. -
ISSN:: 2076-0817
Subject:: Borrelia burgdorferi; DNA; Ixodes scapularis; Lyme disease; Plestiodon; geographical distribution; glass; herpetofauna; lizards; ticks; Florida
Abstract:: ... Host associations of the tick vector for Lyme Borreliosis, Ixodes scapularis, differ across its geographic range. In Florida, the primary competent mammalian host of Lyme disease is not present but instead has other small mammals and herpetofauna that I. scapularis can utilize. We investigated host–tick association for lizards, the abundance of ticks on lizards and the prevalence of Borrelia burgd ...
DOI:: 10.3390/pathogens10080999
: https://doi.org/10.3390/pathogens10080999

21. Taxonomic and identification review of adventive Fiorinia Targioni Tozzetti (Hemiptera, Coccomorpha, Diaspididae) of the United States

Author:: Muhammad Z. Ahmed; Matthew R. Moore; Eric A. Rohrig; Cindy L. McKenzie; Di Liu; Jinian Feng; Benjamin B. Normark; Douglass R. Miller
Source:: ZooKeys 2021 v.1065 pp. 141-203
ISSN:: 1313-2970
Subject:: DNA; adults; diagnostic techniques; monophyly; pest management; surveys; Florida
Abstract:: ... This work provides general descriptions, illustrations, molecular diagnostic data, taxonomic keys, slide mounting recommendations, and Florida distribution records for Fiorinia Targioni Tozzetti species occurring in the USA. Species treated are F. externa Ferris, F. fioriniae (Targioni Tozzetti), F. japonica Kuwana, F. pinicola Maskell, F. phantasma Cockerell & Robinson, F. proboscidaria Green, an ...
DOI:: 10.3897/zookeys.1065.69171
: https://doi.org/10.3897/zookeys.1065.69171

22. Use of ITS-1 to Identify Bactrocera dorsalis and Bactrocera occipitalis (Diptera: Tephritidae): A Case Study Using Flies Trapped in California from 2008 to 2018

Author:: Norman B. Barr; Martin Hauser; Jennifer Belcher; David Salinas; Erin Schuenzel; Peter Kerr; Stephen Gaimari
Source:: Florida entomologist 2021 v.104 no.2 pp. 96-106
ISSN:: 1938-5102
Subject:: Bactrocera carambolae; Bactrocera dorsalis; DNA; case studies; entomologists; fruit flies; internal transcribed spacers; intraspecific variation; nucleotides; peptide elongation factors; plant protection; species identification; California; Florida
Abstract:: ... Molecular methods are necessary to diagnose immature life stages of the agricultural pest fruit fly Bactrocera dorsalis (Hendel), and are useful to corroborate identifications based on adults because morphological variation within the species can overlap with congeners. DNA sequencing of the nuclear ribosomal internal transcribed spacer 1 (ITS-1) has been adopted by the International Plant Protect ...
DOI:: 10.1653/024.104.0205
: https://doi.org/10.1653/024.104.0205

23. Using molecular gut content analysis to identify key predators in a classical weed biological control system: a study with Neomusotima conspurcatalis (Lepidoptera: Crambidae)

Author:: Ian M. Jones; Paul T. Madeira; J. Zizah Blair; Ellen C. Lake
Source:: BioControl 2021 v.66 no.6 pp. 825-836
ISSN:: 1386-6141
Subject:: Crambidae; DNA; Lygodium microphyllum; biological control; biological control agents; digestive system; invasive species; predation; surveys; weeds; Florida
Abstract:: ... Predators can severely limit or prevent the establishment of newly introduced biological control agents. Identifying key predators and quantifying their impacts, however, has often proved difficult, especially in cases where the biological control agent has low establishment or detectability. We used molecular gut content analysis to identify predators feeding on Neomusotima conspurcatalis Warren ...
DOI:: 10.1007/s10526-021-10090-x
: https://doi.org/10.1007/s10526-021-10090-x

24. A multi‐state occupancy modelling framework for robust estimation of disease prevalence in multi‐tissue disease systems

Author:: Vratika Chaudhary; Samantha M. Wisely; Felipe A. Hernández; James E. Hines; James D. Nichols; Madan K. Oli
Source:: Journal of applied ecology 2020 v.57 no.12 pp. 2463-2474
ISSN:: 0021-8901
Subject:: DNA; Suid alphaherpesvirus 1; Sus scrofa; applied ecology; blood; disease prevalence; drought; models; monitoring; mucosa; nose; pathogens; probability; public health; swine; Florida
Abstract:: ... Given the public health, economic and conservation implications of zoonotic diseases, their effective surveillance is of paramount importance. The traditional approach to estimating pathogen prevalence as the proportion of infected individuals in the population is biased because it fails to account for imperfect detection. A statistically robust way to reduce bias in prevalence estimates is to obt ...
DOI:: 10.1111/1365-2664.13744
: https://doi.org/10.1111/1365-2664.13744

25. Analysis of Trace Element Concentrations and Antioxidant Enzyme Activity in Muscle Tissue of the Atlantic Sharpnose Shark, Rhizoprionodon terraenovae

Author:: Rachel Somerville; Maya Fisher; Louise Persson; Samantha Ehnert-Russo; James Gelsleichter; Gretchen Bielmyer-Fraser
Source:: Archives of environmental contamination and toxicology 2020 v.79 no.4 pp. 371-390
ISSN:: 0090-4341
Subject:: DNA; Rhizoprionodon; antioxidant enzymes; apoptosis; aquatic food webs; bioaccumulation; biomarkers; cadmium; catalase; coasts; copper; environment; enzyme activity; glutathione peroxidase; glutathione-disulfide reductase; lead; muscle tissues; muscles; mutation; nickel; oxidative stress; pollution; protein denaturation; risk; sampling; sediments; selenium; sharks; silver; superoxide dismutase; toxicology; trophic levels; waterways; zinc; Alabama; Florida; North Carolina; South Carolina
Abstract:: ... Metals occur naturally in the environment; however, anthropogenic practices have greatly increased metal concentrations in waterways, sediments, and biota. Metals pose health risks to marine organisms and have been associated with oxidative stress, which can lead to protein denaturation, DNA mutations, and cellular apoptosis. Sharks are important species ecologically, recreationally, and commercia ...
DOI:: 10.1007/s00244-020-00753-8
: https://doi.org/10.1007/s00244-020-00753-8

26. Bivalve Feeding Responses to Microalgal Bloom Species in the Indian River Lagoon: the Potential for Top-Down Control

Author:: Eve Galimany; Jessica Lunt; Christopher J. Freeman; Jay Houk; Thomas Sauvage; Larissa Santos; Jillian Lunt; Maria Kolmakova; Malcolm Mossop; Arthur Domingos; Edward J. Phlips; Valerie J. Paul
Source:: Estuaries and coasts 2020 v.43 no.6 pp. 1519-1532
ISSN:: 1559-2723
Subject:: Crassostrea virginica; DNA; Mercenaria mercenaria; Nannochloropsis; Perna viridis; algal blooms; chlorophyll; ecosystems; estuaries; filter feeding; microalgae; rivers; taxonomy; Florida
Abstract:: ... In 2011, the Indian River Lagoon, a biodiverse estuary in eastern Florida (USA), experienced an intense microalgal bloom with disastrous ecological consequences. The bloom included a mix of microalgae with unresolved taxonomy and lasted for 7 months with a maximum concentration of 130 μg chlorophyll a L⁻¹. In 2012, brown tide Aureoumbra lagunensis also bloomed in portions of this estuary, with reo ...
DOI:: 10.1007/s12237-020-00746-9
: https://doi.org/10.1007/s12237-020-00746-9

27. Coloration Patterns of the Tegmina of Mahanarva Spectabilis (Hemiptera: Cercopidae): Biological, Morphological and Genetic Bases

Author:: Ricardo Aquino Borges; Alexander Machado Auad; Marcy Das Graças Fonseca; Cristiano Amancio Vieira Borges; Ana Luisa Sousa Azevedo; Tiago Teixeira De Resende; Gervásio Silva Carvalho; Fernando Rodrigues De Oliveira
Source:: Florida entomologist 2020 v.103 no.3 pp. 376-383
ISSN:: 1938-5102
Subject:: DNA; Mahanarva; color; entomologists; morphometry; phenotype; progeny; Florida
Abstract:: ... This study examines the tegminal coloration pattern and the morphometry of Mahanarva spectabilis (Distant) (Hemiptera: Cercopidae) progeny of crosses between parents with differing wing patterns. Genetic studies were used to investigate whether the different coloration patterns of tegmina have resulted from speciation within the M. spectabilis clade. We crossed M. spectabilis with differing wing p ...
DOI:: 10.1653/024.103.0310
: https://doi.org/10.1653/024.103.0310

28. DNA sequencing and anatomy demonstrate that Pacific Codium simulans is a genetically variable species found in the floras of Bermuda and Florida

Author:: Craig W. Schneider; Daryl W. Lam; Heroen Verbruggen
Source:: Phycological research 2020 v.68 no.1 pp. 98-102
ISSN:: 1322-0829
Subject:: Codium; DNA; nucleotide sequences; sequence analysis; ships; trade; Atlantic Ocean; Bermuda; Florida; Pacific Ocean
Abstract:: ... When DNA sequences from Bermuda plants described as Codium isthmocladum ssp. clavatum and a recent collection from Florida originally thought to be C. decorticatum were analyzed, they were found to be a genetic match to the Pacific Mexican species C. simulans. Historical voucher collections assigned to C. isthmocladum ssp. clavatum show that this Pacific lineage has been present in Bermuda (Atlant ...
DOI:: 10.1111/pre.12396
: https://doi.org/10.1111/pre.12396

29. Detection of Cercopithifilaria bainae infection in shelter dogs and ticks in Oklahoma, USA

Author:: Megan W. Lineberry; Kellee D. Sundstrom; Susan E. Little; Erin M. Stayton; Kelly E. Allen
Source:: Parasites & vectors 2020 v.13 no.1 pp. 216
ISSN:: 1756-3305
Subject:: Cercopithifilaria bainae; DNA; Rhipicephalus sanguineus; arthritis; biopsy; dermatitis; disease vectors; dog diseases; dogs; females; genes; males; microfilariae; morphometry; nucleotide sequences; patients; polymerase chain reaction; ribosomal RNA; skin lesions; ticks; veterinarians; Florida; Italy; Oklahoma
Abstract:: ... BACKGROUND: Cercopithifilaria bainae is a filarioid nematode of dogs. Infection with the parasite was not reported in the USA until 2017, when a dog with skin lesions in Florida was diagnosed. Brown dog ticks, Rhipicephalus sanguineus (sensu lato), are the purported tick vectors, and are widespread in the USA. Therefore, C. bainae is likely present in additional states. Here, we tested dogs and ti ...
DOI:: 10.1186/s13071-020-04089-z
PubMed:: 32334624
PubMed Central:: PMC7183667
: https://doi.org/10.1186/s13071-020-04089-z

30. Dispersal and Land Cover Contribute to Pseudorabies Virus Exposure in Invasive Wild Pigs

Author:: Felipe A. Hernández; Amanda N. Carr; Michael P. Milleson; Hunter R. Merrill; Michael L. Avery; Brandon M. Parker; Cortney L. Pylant; James D. Austin; Samantha M. Wisely
Source:: EcoHealth 2020 v.17 no.4 pp. 498-511
ISSN:: 1612-9202
Subject:: Brucella; DNA; Suid alphaherpesvirus 1; Sus scrofa; brucellosis; canopy; disease transmission; environmental health; invasive species; land cover; landscapes; people; population genetics; regression analysis; shrublands; swine; wildlife; wildlife management; Florida
Abstract:: ... We investigated the landscape epidemiology of a globally distributed mammal, the wild pig (Sus scrofa), in Florida (U.S.), where it is considered an invasive species and reservoir to pathogens that impact the health of people, domestic animals, and wildlife. Specifically, we tested the hypothesis that two commonly cited factors in disease transmission, connectivity among populations and abundant r ...
DOI:: 10.1007/s10393-020-01508-6
: https://doi.org/10.1007/s10393-020-01508-6

31. First Report of Curvularia pseudobrachyspora Causing Leaf Spot on Hemp (Cannabis sativa) in Florida

Author:: M. V. Marin; N.-Y. Wang; J. Coburn; J. Desaeger; N. A. Peres
Source:: Plant disease 2020 v.104 no.12 pp. 3262
ISSN:: 0191-2917
Subject:: Areca catechu; Bayesian theory; Cannabis sativa; Curvularia; DNA; Eleusine indica; alternative hosts; annual weeds; bioplastics; conidia; conidiophores; cryptic species; disease control; field experimentation; fungi; glyceraldehyde-3-phosphate dehydrogenase; greenhouses; growth chambers; hemp; insulating materials; internal transcribed spacers; leaf spot; leaves; mycelium; pathogenicity; pathogens; peptide elongation factors; phylogeny; polysorbates; runoff; statistical analysis; China; Florida; Thailand
Abstract:: ... Hemp (Cannabis sativa L.) is an emerging crop in Florida, with potential use in a variety of commercial and industrial products, including rope, textiles, bioplastics, and insulation. During a field trial in 2019 (October to December) in Wimauma, FL, leaf spots were observed on up to 70% of 1-month-old hemp plants on several varieties, such as ‘Pumma-2’, ‘Eletta Campana’, ‘Carmagnola Selezionata’, ...
DOI:: 10.1094/PDIS-03-20-0546-PDN
: https://doi.org/10.1094/PDIS-03-20-0546-PDN

32. First Report of Fruit Rot of Macadamia sp. Caused by Phytophthora heveae in Yunnan, China

Author:: G. Z. Jiang; L. Tao; H. Yue; X. Y. He
Source:: Plant disease 2020 v.104 no.12 pp. 3268
ISSN:: 0191-2917
Subject:: Anacardium excelsum; DNA; Macadamia; Tetragastris panamensis; avocados; blight; damping off; death; liquids; mycelium; oogonia; oospores; pathogens; pears; plastic film; relative humidity; root rot; sporangia; starch; zoospores; Australia; Brazil; China; Florida
Abstract:: ... In May 2018, a fruit rot disease was found in a Macadamia plantation in Jinghong, Yunnan, China. According to observation, the disease mainly occurred near the ground fruit, and the incidence rate of fruit close to the ground was 20%, and the fruit became brown and necrotic after infection. At present, the yield loss caused by the disease is less than 1%. Phytophthora was isolated from the infecte ...
DOI:: 10.1094/PDIS-11-19-2344-PDN
: https://doi.org/10.1094/PDIS-11-19-2344-PDN

33. First Report of Fusarium oxysporum f. sp. lactucae Causing Vascular Wilt of Lettuce in Florida

Author:: J. Murray; R. N. Raid; C. F. Miller; G. Sandoya-Miranda
Source:: Plant disease 2020 v.104 no.11 pp. 3069
ISSN:: 0191-2917
Subject:: DNA; Fusarium oxysporum f. sp. lactucae; Fusarium wilt; Lactuca sativa; chlorosis; conidia; cultivars; culture media; death; discoloration; disease severity; dissection; greenhouses; industry; leaves; lettuce; mycelium; pathogenicity; sand; seeds; soil; tap roots; Arizona; California; Europe; Florida
Abstract:: ... Florida is the third largest producer of lettuce (Lactuca sativa L.) in the United States. In March and April 2017, lettuce exhibiting signs of wilt and chlorosis was observed in commercial fields in Florida’s Everglade’s Agricultural Area, indicating infection with Fusarium oxysporum f. sp. lactucae (FOL). Affected fields were planted with the iceberg cultivar ‘Chosen’, and wilt incidence was as ...
DOI:: 10.1094/PDIS-12-19-2625-PDN
: https://doi.org/10.1094/PDIS-12-19-2625-PDN

34. First Report of Leaf Spot of Alcea rosea Caused by Alternaria alternata in Italy

Author:: A. Garibaldi; G. Gilardi; S. Matic; I. Luongo; M. L. Gullino
Source:: Plant disease 2020 v.104 no.2 pp. 588
ISSN:: 0191-2917
Subject:: Alcea rosea; Alternaria alternata; Alternaria tenuissima; DNA; agar; carrots; common names; conidia; culture media; fungi; gardens; host range; internal transcribed spacers; latitude; leaf spot; leaves; longitude; mycelium; necrosis; ornamental plants; pathogen identification; pathogenicity; phylogeny; plastic bags; polygalacturonase; polymerase chain reaction; potatoes; relative humidity; spraying; spring; streptomycin; sulfates; temperature; Cuba; Florida; Italy
Abstract:: ... Alcea rosea L. (common name hollyhock) is an ornamental plant in the family Malvaceae, typically grown in parks and gardens. During spring 2019, extensive necrosis was observed on leaves of plants grown in a private garden near Biella in northern Italy (45.6121660 latitude and 8.0562970 longitude). The disease affected approximately 50% of about 100 plants, grown under high relative humidity at te ...
DOI:: 10.1094/PDIS-08-19-1614-PDN
: https://doi.org/10.1094/PDIS-08-19-1614-PDN

35. First Report of Poinsettia Wilt Caused by Amphobotrys ricini (syn. Botryotinia ricini) in Florida

Author:: Charles Krasnow; Ana Maria Bocsanczy; Lee Bloomcamp; David Norman
Source:: Plant disease 2020 v.104 no.11 pp. 3064
ISSN:: 0191-2917
Subject:: Botryotinia; Botrytis cinerea; DNA; Euphorbia milii; Euphorbia pulcherrima; RNA; agar; castor beans; conidia; conidiophores; culture media; disease severity; ethanol; genes; glucose; glyceraldehyde; greenhouses; heat shock proteins; mycelium; pathogenicity; pathogens; sclerotia; stem rot; strawberries; Florida; Louisiana
Abstract:: ... Poinsettia (Euphorbia pulcherrima) plants exhibiting wilt symptoms were observed in two large research greenhouses at the University of Florida, Gainesville, in 2019. Disease symptoms were observed on a limited number (<5%) of plants during a period of high temperatures, and diseased plants had continuously been rogued during the growing season. Shoots of symptomatic plants were wilting with branc ...
DOI:: 10.1094/PDIS-04-20-0891-PDN
: https://doi.org/10.1094/PDIS-04-20-0891-PDN

36. First Report of Sclerotinia sclerotiorum Causing Strawberry Fruit Rot in Florida

Author:: M. V. Marin; N. A. Peres
Source:: Plant disease 2020 v.104 no.12 pp. 3250
ISSN:: 0191-2917
Subject:: DNA; Fragaria ananassa; Sclerotinia sclerotiorum; apothecia; asci; ascospores; crown rot; cultivars; discoloration; disease incidence; eggs; fruits; inoculation methods; inoculum; internal transcribed spacers; mycelium; pathogenicity; pathogens; sclerotia; sensation; strawberries; vegetables; California; England; Florida; Israel; North Carolina; Scotland
Abstract:: ... During the 2019 to 2020 Florida strawberry season (October to April), a strawberry (Fragaria × ananassa) fruit rot was observed in two fields (Plant City and Wimauma, FL) with up to 5% incidence on short-day cultivars Sensation Florida127 and Florida Brilliance. Symptoms on pink and ripe fruit consisted of softening, discoloration, watery rot with white fuzzy mycelium, and initial sclerotium forma ...
DOI:: 10.1094/PDIS-04-20-0879-PDN
: https://doi.org/10.1094/PDIS-04-20-0879-PDN

37. Haplaxius crudus (Hemiptera: Cixiidae) Transmits the Lethal Yellowing Phytoplasmas, 16SrIV, to Pritchardia pacifica Seem. & H.Wendl (Arecaceae) in Yucatan, Mexico

Author:: J-L Dzido; R Sánchez; M Dollet; J-F Julia; M Narvaez; S Fabre; C Oropeza
Source:: Neotropical entomology 2020 v.49 no.6 pp. 795-805
ISSN:: 1519-566X
Subject:: Cixiidae; DNA; Neotropics; Phytoplasma; Pritchardia; cages; coconuts; entomology; phylogeny; restriction fragment length polymorphism; Caribbean; Florida; Mexico
Abstract:: ... Lethal yellowing (LY) affects several palm species in the Americas. It is caused by 16SrIV group phytoplasmas. In Florida (USA), LY was shown to be transmitted by the planthopper Haplaxius crudus (Van Duzee) (Hemiptera, Cixiidae) to different palm species, including Pritchardia pacifica Seem . & H. Wendl . (Arecaceae) in insect-proof cage experiments in the 1980s, a result that had never been repr ...
DOI:: 10.1007/s13744-020-00799-2
: https://doi.org/10.1007/s13744-020-00799-2

38. Implementing Low-Cost, High Accuracy DNA Barcoding From Single Molecule Sequencing to Screen Larval Tephritid Fruit Flies Intercepted at Ports of Entry

Author:: Ivonne J. Garzón-Orduña; Scott M. Geib; Lisa Ledezma; Forest T. Bremer; Norman B. Barr
Source:: Annals of the Entomological Society of America 2020 v.113 no.4 pp. 288-297
ISSN:: 0013-8746
Subject:: DNA; DNA barcoding; Tephritidae; air transportation; fruit flies; fruits; introduced plants; California; Florida
Abstract:: ... Exotic fruit flies in the family Tephritidae pose a threat to U.S. agriculture and natural resources. As part of ongoing fruit fly detection and exclusion programs, invasive fruit fly adults are periodically trapped in and introduced populations are eradicated from two major fruit producing states: California and Florida. Although the pathways used by flies to enter these states are not clear, one ...
DOI:: 10.1093/aesa/saz071
: https://doi.org/10.1093/aesa/saz071

39. Morphological, Molecular, and Biogeographic Evidence for Specific Recognition of Euthamia hirtipes and Euthamia scabra (Asteraceae, Astereae)

Author:: Marisa B. Szubryt; Lowell E. Urbatsch; Yalma L. Vargas-Rodriguez; David Barfknecht; Kurt M. Neubig
Source:: Systematic botany 2020 v.45 no.3 pp. 658-667
ISSN:: 0363-6445
Subject:: Asteraceae; DNA; coasts; geographical distribution; hybrids; niches; phylogeny; rivers; sand fraction; summer; Alabama; Canada; Florida; Louisiana; New Jersey
Abstract:: ... The number and identity of species within Euthamia (Asteraceae, Astereae) have varied considerably among taxonomic treatments. Euthamia graminifolia (L.) Nutt. is often treated broadly, including plants from the northern and eastern United States and Canada, including the Atlantic and Gulf Coasts. Broad-leaved, largely glabrous plants from New Jersey to the Florida Panhandle have been inconsistent ...
DOI:: 10.1600/036364420X15935294613446
: https://doi.org/10.1600/036364420X15935294613446

40. Pathogen Surveillance and Detection of Ranavirus (Frog virus 3) in Translocated Gopher Tortoises (Gopherus polyphemus)

Author:: Rebecca A. Cozad; Terry M. Norton; Matthew J. Aresco; Matthew C. Allender; Sonia M. Hernandez
Source:: Journal of wildlife diseases 2020 v.56 no.3 pp. 679-683
ISSN:: 0090-3558
Subject:: DNA; Frog virus 3; Gopherus polyphemus; Mycoplasma agassizii; blood; digestive tract; epidemiology; frogs; gophers; monitoring; mortality; necropsy; pathogens; quantitative polymerase chain reaction; viruses; wildlife; Florida
Abstract:: ... Emerging pathogens may pose additional threats to already vulnerable populations of chelonians, such as gopher tortoises (Gopherus polyphemus). In response to a mortality event on a translocation site in northwest Florida, US during 2013–15, 13 gopher tortoises were necropsied and their tissues were screened for 12 pathogens, including Mycoplasma agassizii, Mycoplasma testudineum, and Frog virus 3 ...
DOI:: 10.7589/2019-02-053
: https://doi.org/10.7589/2019-02-053

41. Revealing the Diet of Generalist Insect Predators in Strawberry Fields: Not Only Pests, But Other Predators Beware

Author:: K.L. Krey; W.R. Cooper; J.M. Renkema
Source:: Environmental entomology 2020 v.49 no.6 pp. 1300-1306
ISSN:: 0046-225X
Subject:: Chrysoperla; DNA; Dolichopodidae; Drosophilidae; Geocoris; biological control; diet; ecosystem services; entomology; pest management; pests; predation; strawberries; Florida
Abstract:: ... Generalist invertebrate predators contribute to pest management in agriculture, providing an important ecosystem service, particularly in organically managed fields. DNA-based methods to study food webs and feeding interactions in unrestricted field conditions have transformed dietary analysis of generalist predators. In this study, we used MiSeq next-generation sequencing (NGS) technology and uni ...
DOI:: 10.1093/ee/nvaa125
: https://doi.org/10.1093/ee/nvaa125

42. A Survey of Tick-Borne Bacterial Pathogens in Florida

Author:: Carrie E. De Jesus; Claudia Ganser; William H. Kessler; Zoe S. White; Chanakya R. Bhosale; Gregory E. Glass; Samantha M. Wisely
Source:: Insects 2019 v.10 no.9 pp. -
ISSN:: 2075-4450
Subject:: Amblyomma americanum; Amblyomma maculatum; Anaplasma; Borrelia burgdorferi; Borrelia lonestari; DNA; Dermacentor variabilis; Ehrlichia ewingii; Ixodes scapularis; Rickettsia parkeri; climatic zones; endosymbionts; humans; pathogens; polymerase chain reaction; surveys; tick-borne diseases; ticks; virulent strains; Florida
Abstract:: ... Within the past three decades, new bacterial etiological agents of tick-borne disease have been discovered in the southeastern U.S., and the number of reported tick-borne pathogen infections has increased. In Florida, few systematic studies have been conducted to determine the presence of tick-borne bacterial pathogens. This investigation examined the distribution and presence of tick-borne bacter ...
DOI:: 10.3390/insects10090297
PubMed:: 31540253
PubMed Central:: PMC6780285
: https://doi.org/10.3390/insects10090297

43. Culicoides species community composition and infection status with parasites in an urban environment of east central Texas, USA

Author:: Estelle Martin; Elaine Chu; Phillip Shults; Andrew Golnar; Dustin A. Swanson; Jamie Benn; Dongmin Kim; Peter Schneider; Samantha Pena; Cassie Culver; Matthew C. I. Medeiros; Sarah A. Hamer; Gabriel L. Hamer
Source:: Parasites & vectors 2019 v.12 no.1 pp. 39
ISSN:: 1756-3305
Subject:: Culicoides; DNA; Haemoproteus; Onchocercidae; animal and human health; biogeography; community structure; habitats; hemoparasites; species richness; subtropics; urban areas; Bahamas; Florida; South Carolina; Texas
Abstract:: ... BACKGROUND: Despite their importance as vectors of zoonotic parasites that can impact human and animal health, Culicoides species distribution across different habitat types is largely unknown. Here we document the community composition of Culicoides found in an urban environment including developed and natural sites in east central Texas, a region of high vector diversity due to subtropical clima ...
DOI:: 10.1186/s13071-018-3283-9
PubMed:: 30651144
PubMed Central:: PMC6335769
: https://doi.org/10.1186/s13071-018-3283-9

44. Efficacy of eDNA as an early detection indicator for Burmese pythons in the ARM Loxahatchee National Wildlife Refuge in the Greater Everglades Ecosystem

Author:: Margaret E. Hunter; Gaia Meigs-Friend; Jason A. Ferrante; Brian J. Smith; Kristen M. Hart
Source:: Ecological indicators 2019 pp. -
ISSN:: 1470-160X
Subject:: DNA; Python bivittatus; boats; conservation areas; droplets; ecosystems; environmental indicators; geographical distribution; habitats; indigenous species; invasive species; lakes; quantitative polymerase chain reaction; rivers; swamps; trapping; Florida
Abstract:: ... Environmental DNA (eDNA) detection of invasive species can be used to inform management decisions by delimiting occupied ranges and estimating detection probabilities. Environmental DNA is shed into the environment through skin cells and bodily fluids and can be detected in water samples collected from lakes, rivers, and swamps. In south Florida, USA, invasive Burmese pythons (Python bivittatus) o ...
DOI:: 10.1016/j.ecolind.2019.02.058
: https://doi.org/10.1016/j.ecolind.2019.02.058

45. Evaluating the taxonomic status of the Great White Heron (Ardea herodias occidentalis) using morphological, behavioral and genetic evidence

Author:: Heather L. McGuire; Sabrina S. Taylor; Frederick H. Sheldon
Source:: Auk 2019 v.136 no.1 pp. 1-18
ISSN:: 1938-4254
Subject:: Ardea herodias; DNA; alleles; birds; ecotones; gene flow; genetic variation; habitats; islands; mating behavior; microsatellite repeats; nesting; nests; parentage; plumage; Caribbean; Florida; Gulf of Mexico
Abstract:: ... The Great White Heron (GWH) has an all-white plumage and occurs in the Gulf of Mexico and Caribbean. Described originally as Ardea occidentalis, it is now considered a subspecies of Great Blue Heron (GBH; A. herodias). GWH and GBH meet in Florida Bay at the southern tip of Florida, providing the opportunity to evaluate their interaction and species status. To this end, we examined size variation a ...
DOI:: 10.1093/auk/uky010
CHORUS:: 10.1093/auk/uky010
: https://doi.org/10.1093/auk/uky010

46. First Report of Crown Rot Caused by Phytopythium helicoides on Strawberry in the Americas

Author:: M. V. Marin; T. Seijo; J. Mertely; N. A. Peres
Source:: Plant disease 2019 v.103 no.10 pp. 2696
ISSN:: 0191-2917
Subject:: Chrysanthemum; DNA; DNA primers; Fragaria ananassa; Kalanchoe; agar; ambient temperature; brown rice; chlamydospores; commercial farms; crown rot; cultivars; cytochrome-c oxidase; discoloration; disease control; fungicides; genes; growing season; internal transcribed spacers; mortality; pathogenicity; pathogens; pistachios; root rot; selective media; sporangia; stem rot; strawberries; wilting; zoospores; Florida; Japan; South America
Abstract:: ... During the 2018 to 2019 growing season (October to April), a crown rot causing up to 50% mortality on ‘Florida Radiance’ strawberry (Fragaria × ananassa) was observed in six commercial farms in Florida. The wilted and collapsing plants had symptoms of reddish-brown to dark brown discoloration in the crown. A Pythium-like organism was consistently isolated from symptomatic crowns on the selective m ...
DOI:: 10.1094/PDIS-03-19-0658-PDN
: https://doi.org/10.1094/PDIS-03-19-0658-PDN

47. First Report of Erysiphe fallax Causing Powdery Mildew on Phasey Bean (Macroptilium lathyroides) in the United States

Author:: Bindu Poudel; Shouan Zhang
Source:: Plant health progress 2019 v.20 no.1 pp. 35-37
ISSN:: 1535-1025
Subject:: DNA; Erysiphe; Macroptilium lathyroides; autumn; beans; conidia; fungal growth; leaves; pathogenicity; pathogens; phylogeny; powdery mildew; Florida
Abstract:: ... Phasey bean (Macroptilium lathyroides) is a member of the family Fabaceae and is native to tropical America. During the fall of 2017, the leaves of phasey bean plants in Homestead, FL, showed white powdery fungal growth. The morphological characteristics suggested that the pathogen was powdery mildew. For further identification, genomic DNA was extracted from conidia. BLAST results showed that the ...
DOI:: 10.1094/PHP-11-18-0071-BR
: https://doi.org/10.1094/PHP-11-18-0071-BR

48. First Report of Lasiodiplodia theobromae and Neofusicoccum parvum/N. ribis species Complex Causing Fruit Rot on Strawberry in the United States

Author:: M. S. Oliveira; N.-Y. Wang; M. V. Marin; N. A. Peres
Source:: Plant disease 2019 v.103 no.5 pp. 1037
ISSN:: 0191-2917
Subject:: DNA; DNA primers; Fragaria ananassa; Lasiodiplodia theobromae; Neofusicoccum parvum; Neofusicoccum ribis; Pinus; blight; conidia; conifer needles; discoloration; disease incidence; fruit quality; fruits; fungi; hosts; mycelium; pathogenicity; peptide elongation factors; plant rots; pomegranates; postharvest diseases; pycnidia; relative humidity; stem cankers; strawberries; Brazil; Florida
Abstract:: ... During the last three seasons, approximately 1% of strawberry (Fragaria × ananassa) with symptoms of fruit softening and discoloration were observed in three fields in Florida. Fruit eventually became black and mummified with pycnidia embedded in the tissue that produced hyaline, aseptate conidia. Eleven isolates recovered from infected tissue were purified by growing them on 2% water agar (WA) wi ...
DOI:: 10.1094/PDIS-09-18-1531-PDN
: https://doi.org/10.1094/PDIS-09-18-1531-PDN

49. First Report of Southern Root-Knot Nematode, Meloidogyne incognita, on Large Crabgrass, Digitaria sanguinalis, in China

Author:: W. W. Song; X. Shi; N. W. Qi; C. Liang; F. M. Duan; H. H. Zhao
Source:: Plant disease 2019 v.103 no.10 pp. 2694
ISSN:: 0191-2917
Subject:: DNA; Digitaria sanguinalis; Meloidogyne arenaria; Meloidogyne incognita; Meloidogyne javanica; Oriental traditional medicine; crop rotation; crops; eggs; females; galls; greenhouses; growth chambers; juveniles; males; plant diseases and disorders; population density; races; root systems; root-knot nematodes; roots; seedlings; stylets; ultraviolet radiation; vegetables; weeds; China; Florida
Abstract:: ... Large crabgrass, Digitaria sanguinalis (L.) Scop., is a weed seriously damaging agricultural production throughout tropical and temperate regions of the world (King and Oliver 1994). However, in China, D. sanguinalis is used as a traditional Chinese medicine that may have beneficial health effects on both lung and eyesight (Hu et al. 1999). In July 2017, numerous plants of D. sanguinalis with smal ...
DOI:: 10.1094/PDIS-03-19-0641-PDN
: https://doi.org/10.1094/PDIS-03-19-0641-PDN

50. Genetic Variation among Island and Continental Populations of Burrowing Owl (Athene cunicularia) Subspecies in North America

Author:: Alberto Macías-Duarte; Courtney J. Conway; Geoffrey L. Holroyd; Héctor E. Valdez-Gómez; Melanie Culver
Source:: Journal of raptor research 2019 v.53 no.2 pp. 127-133
ISSN:: 0892-1016
Subject:: Athene cunicularia; DNA; genetic drift; genetic variation; geographical distribution; loci; microsatellite repeats; Florida; Mexico
Abstract:: ... Burrowing Owls (Athene cunicularia) have a large geographic range spanning both North and South America and resident populations occur on many islands in the eastern Pacific Ocean and the Caribbean Sea. Many owl populations are isolated and disjunct from other populations, but studies on genetic variation within and among populations are limited. We characterized DNA microsatellite variation in po ...
DOI:: 10.3356/JRR-18-00002
: https://doi.org/10.3356/JRR-18-00002

51. Melanothamnus maniticola sp. nov. (Ceramiales, Rhodophyta): an epizoic species evolved for living on the West Indian Manatee

Author:: Karen A. Woodworth; Thomas A. Frankovich; David W. Freshwater
Source:: Journal of phycology 2019 v.55 no.6 pp. 1239-1245
ISSN:: 0022-3646
Subject:: DNA; Rhodomelaceae; Trichechus manatus; macroalgae; new species; nucleotide sequences; pericarp; sea turtles; thallus; Florida
Abstract:: ... Over 35 macroalgae have been documented growing epizoically on sea turtles, and macroalgae are also known to grow on the West Indian Manatee, but the number and identity of these latter species have not been determined. Analysis of DNA sequences of 12 samples collected from different manatees captured in three areas of Florida indicated that they represented a single undescribed species within the ...
DOI:: 10.1111/jpy.12912
: https://doi.org/10.1111/jpy.12912

52. Mycotic Rhinitis and Sinusitis in Florida Horses

Author:: Sunil Nivrutti More; Oscar Hernandez; William L. Castleman
Source:: Veterinary pathology 2019 v.56 no.4 pp. 586-598
ISSN:: 1544-2217
Subject:: Alternaria alternata; Aspergillus; Curvularia lunata; DNA; DNA barcoding; Exserohilum; Fusarium solani; Oomycetes; animal pathology; biopsy; fungi; histopathology; horses; mixed infection; mucosa; nose; pathogens; polymerase chain reaction; pythiosis; rhinitis; sinusitis; tropics; Florida
Abstract:: ... Rhinitis and sinusitis caused by fungal pathogens were studied in biopsy samples submitted from 52 horses distributed throughout subtropical and tropical regions of Florida. Methods included routine histopathology as well as polymerase chain reaction (PCR) with panfungal/panoomycete primers and DNA sequencing on extracted DNA (DNA barcoding). Granulomatous, pyogranulomatous, and fibrinopurulent le ...
DOI:: 10.1177/0300985818817046
: https://doi.org/10.1177/0300985818817046

53. Patterns of genetic divergence among populations of Aedes aegypti L. (Diptera: Culicidae) in the southeastern USA

Author:: Kristen A. Hopperstad; Michael H. Reiskind; Paul E. Labadie; Martha O. Burford Reiskind
Source:: Parasites & vectors 2019 v.12 no.1 pp. 511
ISSN:: 1756-3305
Subject:: Aedes aegypti; Aedes albopictus; DNA; adults; cities; eggs; emerging diseases; gene flow; genetic markers; genetic recombination; genetic variation; genotyping; models; ovitraps; pesticide resistance; population structure; public health; rearing; vectorial capacity; Florida; Georgia; Louisiana
Abstract:: ... BACKGROUND: The yellow fever mosquito, Aedes aegypti is a public health concern in the USA, especially in the wake of emergent diseases such as Zika and chikungunya. Aedes aegypti populations dwindled after the invasion of Aedes albopictus in the 1980s and many populations were extirpated. However, in some areas Ae. aegypti persisted in small populations and there are reports of recent resurgences ...
DOI:: 10.1186/s13071-019-3769-0
PubMed:: 31666113
PubMed Central:: PMC6822358
: https://doi.org/10.1186/s13071-019-3769-0

54. Pilot Study on the Effect of Cocaine Use on the Intestinal Microbiome and Metabolome and Inflammation in HIV-Infected Adults in the Miami Adult Studies in HIV (MASH) Cohort (P13-027-19)

Author:: Sabrina Martinez; Adriana Campa; Giri Narasimhan; Danielle Portuando; Leslie Seminario; Juphshy Jasmin; Marianna Baum
Source:: Current developments in nutrition 2019 v.3 no.Supplement_1 pp. -
ISSN:: 2475-2991
Subject:: DNA; HIV infections; Human immunodeficiency virus; adults; analytical kits; antibiotics; blood plasma; cocaine; cross-sectional studies; drugs; dysbiosis; enzyme-linked immunosorbent assay; fasting; feces; food intake; food security; gas chromatography-mass spectrometry; healthy eating habits; inflammation; interleukin-6; intestinal microorganisms; intestines; liquid chromatography; metabolism; metabolome; ribosomal RNA; sequence analysis; sexual behavior; survival rate; Florida
Abstract:: ... Drug use disorders play a major role in the HIV epidemic, and survival rates of HIV infected drug users are significantly lower than non-users. Cocaine use, the most prevalent drug of abuse in Miami, Florida, has also been associated with a pro-inflammatory state and recently with imbalances in the intestinal microbiota as compared to cocaine non-use. Cocaine users have higher levels of inflammati ...
DOI:: 10.1093/cdn/nzz036.P13-027-19
: https://doi.org/10.1093/cdn/nzz036.P13-027-19

55. Survey of Texas Bay Systems for the Oyster Parasites Bonamia spp., Haplosporidium nelsoni, and Perkinsus marinus

Author:: Hailey M. Boeck; Susan E. Laramore; David S. Portnoy; John Scarpa
Source:: Journal of shellfish research 2019 v.38 no.3 pp. 557-564
ISSN:: 0730-8000
Subject:: Crassostrea virginica; DNA; Haplosporidium nelsoni; Isognomon alatus; Ostrea; Perkinsus marinus; etiological agents; genetic variation; histology; monitoring; oysters; parasites; polymerase chain reaction; surveys; Florida; Texas
Abstract:: ... Texas Parks and Wildlife Department restricts movement of eastern oysters Crassostrea virginica among Texas bay systems because of potential disease transfer and genetic differences in natural oyster stocks. Oyster diseases, such as bonamiosis, which was found serendipitously in 2007 in Florida waters, and MSX (Haplosporidium nelsoni) have not been characterized in Texas bays. Therefore, it is pru ...
DOI:: 10.2983/035.038.0306
: https://doi.org/10.2983/035.038.0306

56. Temporal patterns of entomopathogenic nematodes in Florida citrus orchards: Evidence of natural regulation by microorganisms and nematode competitors

Author:: Raquel Campos-Herrera; Robin J. Stuart; Ekta Pathak; Fahiem E. El-Borai; Larry W. Duncan
Source:: Soil biology & biochemistry 2019 v.128 pp. 193-204
ISSN:: 0038-0717
Subject:: Acrobeloides; Citrus; DNA; Heterorhabditis indica; Paenibacillus; Purpureocillium lilacinum; Steinernema diaprepesi; bacteria; ectoparasites; endospores; entomopathogenic nematodes; natural enemies; nematophagous fungi; orchards; population growth; predation; quantitative polymerase chain reaction; regression analysis; soil depth; soil horizons; soil pH; spatial distribution; surveys; Florida
Abstract:: ... The abundance of three entomopathogenic nematode (EPN) species, seven species of nematophagous fungi, free living nematode competitors of EPNs and a bacterial ectoparasite of EPNs were monitored during approximately two years in four Florida citrus orchards. The objective of the surveys was to identify natural enemies that potentially regulate the temporal abundance of naturally occurring EPNs by ...
DOI:: 10.1016/j.soilbio.2018.10.012
CHORUS:: 10.1016/j.soilbio.2018.10.012
: https://doi.org/10.1016/j.soilbio.2018.10.012

57. Using Noninvasive Genetic Sampling to Survey Rare Butterfly Populations

Author:: Caroline Storer; Jaret Daniels; Lei Xiao; Kristin Rossetti
Source:: Insects 2019 v.10 no.10 pp. -
ISSN:: 2075-4450
Subject:: DNA; breeding; butterflies; chorion; citizen scientists; eggs; nondestructive methods; sampling; sequence analysis; surveys; Florida
Abstract:: ... Advances in nondestructive genetic sampling techniques continue to offer new opportunities for studying organisms, particularly those of conservation concern where more traditional invasive sampling methods are often not available. As part of a proof-of-concept, we investigated the effectiveness of using the chorion from residual butterfly egg debris as a source of viable genetic material for anal ...
DOI:: 10.3390/insects10100311
PubMed:: 31547512
PubMed Central:: PMC6835262
: https://doi.org/10.3390/insects10100311

58. Evaluation of the novel crAssphage marker for sewage pollution tracking in storm drain outfalls in Tampa, Florida

Author:: Warish Ahmed; Aldo Lobos; Jacob Senkbeil; Jayme Peraud; Javier Gallard; Valerie J. Harwood
Source:: Water research 2018 v.131 pp. 142-150
ISSN:: 0043-1354
Subject:: Bacteroides; DNA; bacteriophages; cross reaction; feces; genes; humans; pollution; poultry manure; quantitative polymerase chain reaction; rain; sewage; storms; stormwater; surface water; Florida
Abstract:: ... CrAssphage are recently-discovered DNA bacteriophages that are prevalent and abundant in human feces and sewage. We assessed the performance characteristics of a crAssphage quantitative PCR (qPCR) assay for quantifying sewage impacts in stormwater and surface water in subtropical Tampa, Florida. The mean concentrations of crAssphage in untreated sewage ranged from 9.08 to 9.98 log10 gene copies/L. ...
DOI:: 10.1016/j.watres.2017.12.011
: https://doi.org/10.1016/j.watres.2017.12.011

59. Evidence of pseudorabies virus shedding in feral swine (sus scrofa) populations of florida, usa

Author:: Felipe A. Hernández; Katherine A. Sayler; Courtney Bounds; Michael P. Milleson; Amanda N. Carr; Samantha M. Wisely
Source:: Journal of wildlife diseases 2018 v.54 no.1 pp. 45-53
ISSN:: 0090-3558
Subject:: nose; quantitative polymerase chain reaction; Suid alphaherpesvirus 1; risk; wildlife; antibody formation; feral animals; monitoring; antibodies; DNA; serology; Sus scrofa; financial economics; viruses; immunologic techniques; viral shedding; blood; pathogens; swine; Florida
Abstract:: ... : Feral swine (Sus scrofa) are a pathogen reservoir for pseudorabies virus (PrV). The virus can be fatal to wildlife and contributes to economic losses in the swine industry worldwide. National surveillance efforts in the US use serology to detect PrV-specific antibodies in feral swine populations, but PrV exposure is not a direct indicator of pathogen transmission among conspecifics or to non-sui ...
DOI:: 10.7589/2017-04-071
: https://doi.org/10.7589/2017-04-071

60. First Report of Alternaria Leaf Spot of Cilantro (Coriandrum sativum) Caused by Alternaria dauci in the United States

Author:: B. Poudel; S. Zhang
Source:: Plant disease 2018 v.102 no.4 pp. 822
ISSN:: 0191-2917
Subject:: Alternaria dauci; Coriandrum sativum; DNA; agar; allergens; ambient temperature; carrots; celery; cilantro; conidia; cuisine; culture media; ethanol; fungi; genes; glyceraldehyde-3-phosphate dehydrogenase; inoculum; leaf spot; leaves; mycelium; parsley; pathogenicity; photoperiod; reverse transcriptase polymerase chain reaction; sodium hypochlorite; spraying; tail; tissues; Florida; Mauritius; Puerto Rico
Abstract:: ... Cilantro (Coriandrum sativum), widely used in different types of cuisine worldwide, is an annual herb in the family Apiaceae, which contains over 3,000 species including parsley, celery, and carrot. In January 2017, small brown spots with a white center were observed on leaves of cilantro in a plantation in Homestead, FL. Approximately 15% of the plants in the field were infested. Small pieces (5 ...
DOI:: 10.1094/PDIS-08-17-1297-PDN
: https://doi.org/10.1094/PDIS-08-17-1297-PDN

61. First Report of Diplodia sapinea and Diplodia scrobiculata Causing an Outbreak of Tip Blight on Slash Pine in Florida

Author:: C. A. Paez; J. A. Smith
Source:: Plant disease 2018 v.102 no.8 pp. 1657
ISSN:: 0191-2917
Subject:: DNA; DNA primers; Diplodia pinea; Pinus elliottii; agar; air drying; autumn; blight; branches; cambium; culture media; decline; dieback; experimental design; financial economics; fungi; greenhouses; internal transcribed spacers; mortality; mycelium; pathogens; plastics; sapwood; seedlings; shoot tips; sodium hypochlorite; trees; virulence; wilting; Florida; Michigan
Abstract:: ... In the fall of 2012, extensive dieback and mortality were observed on slash pine stands along a highway in Orange County, FL (28°32′11.3″N, 81°33′30.1″W). Branches from symptomatic trees were collected and transported in a cooler to the laboratory. Small sapwood pieces from the margins of lesions from the symptomatic branches with resinous cankers on the stem were excised, surface sterilized in 2. ...
DOI:: 10.1094/PDIS-07-17-1093-PDN
: https://doi.org/10.1094/PDIS-07-17-1093-PDN

62. First Report of Phytophthora nicotianae Causing Crown Rot of Strawberry in the United States

Author:: M. V. Marin; T. Seijo; M. S. Oliveira; E. Zuchelli; J. Mertely; N. A. Peres
Source:: Plant disease 2018 v.102 no.7 pp. 1463
ISSN:: 0191-2917
Subject:: DNA; Fragaria ananassa; Phytophthora cactorum; Phytophthora nicotianae; agar; chlamydospores; commercial farms; crown rot; cultivars; discoloration; disease control; fungicides; genes; greenhouses; internal transcribed spacers; leather; mating types; mists; mortality; mycelium; oogonia; pathogenicity; pathogens; polymerase chain reaction; roots; selective media; sporangia; strawberries; tissues; zoospores; Florida; Japan; South Korea
Abstract:: ... Phytophthora crown rot is an important disease of strawberry (Fragaria × ananassa) worldwide. In Florida, Phytophthora cactorum is the most common causal agent. During the 2015–2016 and 2016–2017 seasons, eight commercial farms in west-central Florida had up to 25% strawberry plants with wilt symptoms and dark reddish-brown discoloration of crowns. Isolations were made on P₅ARP selective medium (E ...
DOI:: 10.1094/PDIS-08-17-1333-PDN
: https://doi.org/10.1094/PDIS-08-17-1333-PDN

63. First Report of Rust Caused by Puccinia arachidis on Peanut in South Carolina

Author:: D. J. Anco; H. Wang
Source:: Plant disease 2018 v.102 no.3 pp. 676
ISSN:: 0191-2917
Subject:: Arachis hypogaea; DNA; Puccinia arachidis; acreage; basidia; chlorothalonil; genome; growing season; hosts; industry; leaves; nucleotide sequences; overwintering; peanuts; polymerase chain reaction; storms; tebuconazole; urediniospores; Cuba; Florida; Georgia; Mexico; North Carolina; South Carolina; Texas
Abstract:: ... Puccinia arachidis Speg. (phylum Basidiomycota, class Pucciniomycetes) causes rust on peanut (Arachis hypogaea L.) and is a biotroph. In September 2016, several plants exhibiting numerous rust-colored pustules characteristic to peanut rust (Subrahmanyam 1997) on abaxial leaf sides were observed in two plots (12.19 × 12.67 m) at approximately 5% incidence of total leaflets (≈3% severity) in a local ...
DOI:: 10.1094/PDIS-08-17-1245-PDN
: https://doi.org/10.1094/PDIS-08-17-1245-PDN

64. First Report of Tar Spot on Corn (Zea mays) Caused by Phyllachora maydis in Florida, Iowa, Michigan, and Wisconsin

Author:: A. G. McCoy; M. K. Romberg; E. R Zaworski; A. E. Robertson; A. Phibbs; B. D. Hudelson; D. L. Smith; R. L. Beiriger; R. N. Raid; J. M. Byrne; M. I. Chilvers
Source:: Plant disease 2018 v.102 no.9 pp. 1851
ISSN:: 0191-2917
Subject:: DNA; Monographella; Phyllachora; Zea mays; asci; ascomata; ascospores; corn; crop production; disease severity; fungi; genes; hybrids; internal transcribed spacers; leaves; lodging; mixed infection; oligodeoxyribonucleotides; pathogens; ribosomal RNA; sequence homology; Florida; Illinois; Indiana; Iowa; Michigan; South America; Wisconsin
Abstract:: ... Leaf lesions typical of those reported for tar spot of corn caused by Phyllachora maydis Maubl. were observed in commercial corn hybrids (Zea mays L.). In 2016, symptoms were observed in Jones County, IA, Palm Beach County, FL, Allegan County, MI, and in Green and Iowa counties, WI. In 2017, symptoms were observed again in Allegan County, MI, and in Grant and Lafayette counties, WI. Leaf signs and ...
DOI:: 10.1094/PDIS-02-18-0271-PDN
: https://doi.org/10.1094/PDIS-02-18-0271-PDN

65. First Report of the Root Lesion Nematode, Pratylenchus bolivianus, on Aspalathus linearis in South Africa

Author:: F. Daramola; R. Knoetze; A. P. Malan
Source:: Plant disease 2018 v.102 no.9 pp. 1860
ISSN:: 0191-2917
Subject:: Aspalathus linearis; DNA; Pratylenchus; acreage; altitude; anti-aging properties; cardiovascular diseases; carrots; ecosystems; exports; females; ferns and fern allies; fynbos; genes; genetic databases; inoculum; intestines; males; markets; morphometry; noninsulin-dependent diabetes mellitus; oats; orchards; pharynx; polymerase chain reaction; potatoes; rhizosphere; ribosomal RNA; risk reduction; root lesion nematodes; roots; seedlings; soil; soil sampling; spermatheca; stylets; surveys; tail; tea; testes; vulva; Andes region; Bolivia; Europe; Florida; South Africa
Abstract:: ... Pratylenchus bolivianus Corbett, 1983 (Nematoda: Pratylenchidae) is an obligate plant-parasitic nematode that was originally described from soil around the roots of oats and potatoes in the Bolivian Andes, at an altitude of about 3,500 m above sea level (Corbett 1983). More recently an amphimictic population of this species was reported and characterized from sword ferns in Florida (Troccoli et al ...
DOI:: 10.1094/PDIS-02-18-0215-PDN
: https://doi.org/10.1094/PDIS-02-18-0215-PDN

66. Histologic characterization of eosinophilic encephalitis in horses in Florida

Author:: Whitney M. Zoll; Dhani Prakoso; Michael Dark; Junjie Liu; Heather Stockdale-Walden; Maureen T. Long
Source:: Journal of veterinary diagnostic investigation v.30 no.3 pp. 442-446
ISSN:: 1943-4936
Subject:: DNA; Neospora hughesi; Parastrongylus cantonensis; Sarcocystis neurona; central nervous system; databases; encephalitis; eosinophils; fungi; histology; horse diseases; horses; parasites; parasitoses; pathogens; quantitative polymerase chain reaction; Florida
Abstract:: ... Eosinophils within the central nervous system are abnormal and are usually associated with fungal or parasitic infections in horses. Causative agents include Halicephalobus gingivalis, Sarcocystis neurona, and Neospora hughesi. Confirmation of these organisms via specific testing is typically not performed, and final diagnoses are often presumptive. With molecular technology, many of these organis ...
DOI:: 10.1177/1040638718763877
PubMed:: 29528809
PubMed Central:: PMC6505826
: https://doi.org/10.1177/1040638718763877

67. Necrotizing interstitial pneumonia and suppurative myocarditis associated with Bartonella henselae infection in three Florida pumas

Author:: Elizabeth J. Elsmo; Heather Fenton; Christopher A. Cleveland; Barbara Shock; Mark Cunningham; Elizabeth W. Howerth; Michael J. Yabsley
Source:: Journal of veterinary diagnostic investigation v.30 no.5 pp. 728-732
ISSN:: 1943-4936
Subject:: Bartonella henselae; DNA; Puma concolor coryi; captive animals; edema; genes; histiocytes; histopathology; immunohistochemistry; in situ hybridization; lungs; myocarditis; necropsy; pneumonia; polymerase chain reaction; sequence analysis; staining; transmission electron microscopy; Florida
Abstract:: ... Three Florida pumas (Puma concolor coryi) that had spent time in captivity prior to being released in the wild were found exhibiting respiratory signs and reluctance to move. All 3 pumas died shortly after immobilization, despite supportive veterinary care. Significant autopsy findings included necrotizing interstitial pneumonia, with pulmonary edema and hyaline membranes, and suppurative myocardi ...
DOI:: 10.1177/1040638718789226
PubMed:: 30027827
PubMed Central:: PMC6505792
: https://doi.org/10.1177/1040638718789226

68. Pyroaerobiology: the aerosolization and transport of viable microbial life by wildland fire

Author:: Leda N. Kobziar; Melissa R. A. Pingree; Heather Larson; Tyler J. Dreaden; Shelby Green; Jason A. Smith
Source:: Ecosphere 2018 v.9 no.11 pp. e02507
ISSN:: 2150-8925
Subject:: DNA; Pinus palustris; biodiversity; biogeography; biomass; burning; coniferous forests; ecological restoration; ecosystems; environmental factors; forest litter; fuels; fungi; human health; human population; ice; meteorology; morphs; pathogens; public health; smoke; symbionts; wildfires; Florida
Abstract:: ... The field of aerobiology is expanding due to a recognition of the diversity of roles microbes play in both terrestrial and atmospheric ecology. Smoke from global biomass burning has had significant and widespread ecological and human health consequences, but the living component of smoke has received little attention. Microbes aerosolized and transported by wildland fire may have profound effects ...
DOI:: 10.1002/ecs2.2507
CHORUS:: 10.1002/ecs2.2507
: https://doi.org/10.1002/ecs2.2507

69. Screwworm (Diptera: Calliphoridae) in the United States: Response to and Elimination of the 2016–2017 Outbreak in Florida

Author:: Steven R Skoda; Pamela L Phillips; John B Welch
Source:: Journal of medical entomology 2018 v.55 no.4 pp. 777-786
ISSN:: 1938-2928
Subject:: Cochliomyia hominivorax; DNA; Odocoileus virginianus; deer; disease outbreaks; females; financial economics; habitats; image analysis; imagos; larvae; liver; myiasis; pupae; remote sensing; sterile insect technique; trapping; wildlife; Florida
Abstract:: ... Eradicating screwworm, Cochliomyia hominivorax (Coquerel), from continental North American via the sterile insect technique has provided huge economic benefit to livestock producers by eliminating screwworm myiasis. After confirmatory identification of fly samples from infested deer by the USDA National Veterinary Services Laboratory on September 30, 2016, an alert was issued that screwworm myiasi ...
DOI:: 10.1093/jme/tjy049
: https://doi.org/10.1093/jme/tjy049

70. Serological and molecular prevalence of equine piroplasmosis in Western Java, Indonesia

Author:: Arifin Budiman Nugraha; Umi Cahyaningsih; Amrozi Amrozi; Yusuf Ridwan; Srihadi Agungpriyono; Dharmawaty Muhammad Taher; Azirwan Guswanto; Sambuu Gantuya; Dickson Stuart Tayebwa; Bumduuren Tuvshintulga; Thillaiampalam Sivakumar; Naoaki Yokoyama; Ikuo Igarashi
Source:: Veterinary Parasitology: Regional Studies and Reports 2018 v.14 pp. 1-6
ISSN:: 2405-9390
Subject:: Babesia caballi; DNA; Theileria equi; antigens; bioinformatics; blood sampling; blood serum; enzyme-linked immunosorbent assay; equine piroplasmosis; genes; horses; light microscopes; microscopy; molecular cloning; monitoring; nucleotide sequences; parasites; phylogeny; polymerase chain reaction; rearing; sequence analysis; seroprevalence; Florida; Indonesia; Washington (state)
Abstract:: ... Equine piroplasmosis is an economically significant disease caused by Theileria equi and Babesia caballi, which are tick-borne hemoprotozoan parasites. Infections with these parasite species had never been reported in horses in Indonesia. The aim of the present study was to investigate the prevalence of T. equi and B. caballi in horses reared in parts of Western Java, Indonesia. Blood samples were ...
DOI:: 10.1016/j.vprsr.2018.07.009
: https://doi.org/10.1016/j.vprsr.2018.07.009

71. Terrestrial Snake Environmental DNA Accumulation and Degradation Dynamics and its Environmental Application

Author:: Alexander Kucherenko; John E. Herman; Edwin M. Everham III; Hidetoshi Urakawa
Source:: Herpetologica 2018 v.74 no.1 pp. 38-49
ISSN:: 1938-5099
Subject:: DNA; Pantherophis guttatus; biomass; burrows; cameras; cytochrome b; cytochrome-c oxidase; environmental monitoring; genes; invasive species; mitochondria; mitochondrial genome; radio telemetry; refuge habitats; ribosomal RNA; sampling; snakes; soil; soil sampling; Florida
Abstract:: ... There is an increasing need for effective biomonitoring tools that quantify patterns of habitat occupancy by reptile species. Environmental DNA (eDNA) has been regarded as an emerging tool to detect specific target species; however, the dynamics of accumulation and degradation of eDNA in terrestrial environments are poorly understood. This study determines the time required for terrestrial snakes ...
DOI:: 10.1655/Herpetologica-D-16-00088
: https://doi.org/10.1655/Herpetologica-D-16-00088

72. The Alapahoochee watershed microgeographic structure and its potential influence on metal concentrations and genetic structure in the Florida cottonmouth, Agkistrodon piscivorus conanti, within the watershed

Author:: Joseph Ashley Kirkly; Gretchen K. Bielmyer-Fraser; John F. Elder; David L. Bechler
Source:: Environmental monitoring and assessment 2018 v.190 no.4 pp. 220
ISSN:: 0167-6369
Subject:: Agkistrodon piscivorus; DNA; DNA primers; basins; body size; females; genetic analysis; liver; loci; males; nickel; road kills; streams; watersheds; Florida
Abstract:: ... This study examines the microgeographic structure of the Alapahoochee watershed, part of the Suwannee River basin, south-central GA, USA, and relates it to variations in liver metal concentrations and genetic structure of the Florida cottonmouth, Agkistrodon piscivorus conanti. One objective was to determine if liver metal concentrations in A. piscivorus differ between Grand Bay and Mud creeks, wh ...
DOI:: 10.1007/s10661-018-6579-5
: https://doi.org/10.1007/s10661-018-6579-5

73. The issue with using DNA profiling as a sole method for investigating the role of marine biofilms in corrosion of metallic materials

Author:: Zakari Makama; Sukriye Celikkol; Akiko Ogawa; Christine Gaylarde; Iwona Beech
Source:: International biodeterioration & biodegradation 2018 v.135 pp. 33-38
ISSN:: 0964-8305
Subject:: DNA; DNA fingerprinting; Marinobacter; bacterial communities; biofilm; carbon; corrosion; dialysis; electrodes; high-throughput nucleotide sequencing; marine environment; microscopy; seawater; steel; Florida
Abstract:: ... Bacterial community DNA profiles were obtained from surfaces of corroding carbon steel electrodes using next generation sequencing (NGS) Illumina technology. One electrode was exposed to the microbial population present in Key West seawater (KWS), Florida, USA, augmented with Marinobacter J5B1. Another electrode (control) was kept in sterile KWS with the KWS population enclosed within a dialysis t ...
DOI:: 10.1016/j.ibiod.2018.09.007
: https://doi.org/10.1016/j.ibiod.2018.09.007

74. A high-throughput genotyping system combining rapid DNA extraction and high-resolution melting analysis in allo-octoploid strawberry

Author:: S. Lee; Y. H. Noh; J. A. Roach; J. Mangandi; S. Verma; V. M. Whitaker; K. R. Cearley
Source:: Acta horticulturae 2017 no.1156 pp. 89-94
ISSN:: 0567-7572
Subject:: DNA; Phytophthora cactorum; Rosaceae; breeding programs; cost effectiveness; fruit crops; gamma-decalactone; genetic markers; genotyping; leaf spot; loci; marker-assisted selection; melting; models; octaploidy; plant breeding; recalcitrant species; root rot; screening; seedlings; spring; strawberries; Florida
Abstract:: ... The strawberry is an important commodity as well as a model plant species in the Rosaceae family. DNA markers for important traits in octoploid strawberry have increased dramatically in recent years, and are now widely applied for marker-assisted selection (MAS) to assist strawberry breeding. To date, laborious and expensive DNA extraction procedures have been used to achieve sufficient DNA qualit ...
DOI:: 10.17660/ActaHortic.2017.1156.12
: https://doi.org/10.17660/ActaHortic.2017.1156.12

75. Advances in strawberry breeding at the University of Florida

Author:: V. M. Whitaker; S. Lee; L. F. Osorio; S. Verma; J. A. Roach; J. Mangandi; Y. H. Noh; S. Gezan; N. Peres
Source:: Acta horticulturae 2017 no.1156 pp. 1-6
ISSN:: 0567-7572
Subject:: DNA; Glomerella cingulata; Phytophthora cactorum; Xanthomonas fragariae; breeding programs; crossing; crown rot; cultivars; disease resistance; flavor; flowering; fruit quality; gamma-decalactone; genetic traits; genome; germplasm; haplotypes; leaf spot; linkage groups; loci; marker-assisted selection; melting; microsatellite repeats; new variety; odors; parents; quantitative trait loci; root rot; seedlings; strawberries; total soluble solids; Florida
Abstract:: ... In the last three years, the strawberry breeding program at the University of Florida (UF) has sought to implement genomics-assisted breeding. Continuing a long history of cultivar releases for subtropical and Mediterranean climates, development of new cultivars with improved flavor, fruit quality, early yield and disease resistance is being accomplished by combining traditional field breeding wit ...
DOI:: 10.17660/ActaHortic.2017.1156.1
: https://doi.org/10.17660/ActaHortic.2017.1156.1

76. Banisia argutula (Lepidoptera: Thyrididae) is the Dominant Sapodilla Borer in Southern Florida

Author:: Jose I. Martinez; James E. Hayden; John B. Heppner; Jorge E. Peña; Lei Xiao; Daniel Carrillo
Source:: Florida entomologist 2017 v.100 no.1 pp. 57-62
ISSN:: 1938-5102
Subject:: DNA; Lepidoptera; Manilkara zapota; adults; buds; calyx; eggs; females; groves; larvae; leafrollers; leaves; males; mastication; nucleotide sequences; pests; rearing; sapodillas; silk; surveys; Florida
Abstract:: ... Banisia myrsusalis Walker (Lepidoptera: Thyrididae) has been regarded as the most damaging pest of sapodilla, Manilkara zapota (L.) van Royen (Sapotaceae), in Florida, where it causes extensive damage to blooms. Two commercial sapodilla groves were surveyed for lepidopteran eggs and larvae affecting floral buds and mature flowers. All collected specimens were raised in the laboratory until adult e ...
DOI:: 10.1653/024.100.0110
: https://doi.org/10.1653/024.100.0110

77. Borrelia burgdorferi DNA absent, multiple Rickettsia spp. DNA present in ticks collected from a teaching forest in North Central Florida

Author:: Katherine Sayler; Jessica Rowland; Carisa Boyce; Emma Weeks
Source:: Ticks and tick-borne diseases 2017 v.8 no.1 pp. 53-59
ISSN:: 1877-959X
Subject:: Amblyomma americanum; Anaplasma; Borrelia burgdorferi; DNA; Dermacentor variabilis; Ehrlichia; Ixodes scapularis; Lyme disease; Rickettsia; adults; emerging diseases; forests; longitudinal studies; pathogens; polymerase chain reaction; public health; risk; spring; ticks; Florida
Abstract:: ... Tick-borne diseases are an emerging public health threat in the United States. In Florida, there has been public attention directed towards the possibility of locally acquired Borrelia burgdorferi sensu stricto, the causative agent of Lyme disease, in association with the lone star tick. The aim of this study was to determine the prevalence of ticks and the pathogens they carry and potentially tra ...
DOI:: 10.1016/j.ttbdis.2016.09.016
: https://doi.org/10.1016/j.ttbdis.2016.09.016

78. Bovine Papillomavirus DNA and S100 Profiles in Sarcoids and Other Cutaneous Spindle Cell Tumors in Horses

Author:: E. D. Epperson; W. L. Castleman
Source:: Veterinary pathology v.54 no.1 pp. 44-52
ISSN:: 1544-2217
Subject:: Papillomaviridae; animal pathology; diagnostic techniques; staining; immunohistochemistry; horses; DNA; polymerase chain reaction; fibrosarcoma; dermis; histopathology; nerve tissue; Florida
Abstract:: ... Histopathologic differentiation between deep dermal or subcuticular equine sarcoids (ie, nodular sarcoids) and other spindle cell tumors in the dermis and subcutis such as peripheral nerve sheath tumors (PNSTs) can be challenging based on morphologic criteria alone. It has been proposed that polymerase chain reaction (PCR) for bovine papillomavirus (BPV) DNA and S100 immunohistochemistry be used a ...
DOI:: 10.1177/0300985816653169
: https://doi.org/10.1177/0300985816653169

79. Bovine herpesvirus 4 DNA is not detected in free-ranging domestic cats from California, Colorado or Florida

Author:: Elliott Chiu; Ryan M Troyer; Michael R Lappin; Sue VandeWoude
Source:: Journal of feline medicine and surgery v.19 no.2 pp. 235-239
ISSN:: 1532-2750
Subject:: blood sampling; cats; DNA; cattle; polymerase chain reaction; signs and symptoms (animals and humans); urinary tract diseases; blood cells; genes; cat diseases; Bovine gammaherpesvirus 4; thymidine kinase; urinary tract; tissues; pathogens; tissue culture; California; Colorado; Florida
Abstract:: ... Several studies have reported that domestic cats can be naturally infected with bovine herpesvirus 4 (BHV4). Cats experimentally inoculated with BHV4 developed clinical signs involving the urinary tract, leading to the hypothesis that natural infection with BHV4 may be associated with feline lower urinary tract diseases. However, the question of whether BHV4 infection is common in cats remains equ ...
DOI:: 10.1177/1098612X15607586
PubMed:: 26450623
PubMed Central:: PMC5502126
: https://doi.org/10.1177/1098612X15607586

80. Cytogeography of CallisiasectionCuthbertia (Commelinaceae)

Author:: Iwan E. Molgo; Douglas E. Soltis; Pamela S. Soltis
Source:: Comparative cytogenetics 2017 v.11 no.4 pp. 553-577
ISSN:: 1993-078X
Subject:: Callisia; DNA; biodiversity; chromosomes; coastal plains; cytotypes; diploidy; evolution; flow cytometry; geographical distribution; georeferencing; hexaploidy; phytogeography; tetraploidy; Florida; Georgia; North Carolina; South Carolina
Abstract:: ... Determining the distribution of cytotypes across the geographic distribution of polyploid complexes can provide valuable information about the evolution of biodiversity. Here, the phytogeography of cytotypes in section Cuthbertia (Small, 1903) Hunt, 1986 is investigated. A total of 436 voucher specimens was georeferenced; 133 new specimens were collected. Based on flow cytometry data, DNA content ...
DOI:: 10.3897/compcytogen.v11i4.11984
: https://doi.org/10.3897/compcytogen.v11i4.11984

81. DNA and dispersal models highlight constrained connectivity in a migratory marine megavertebrate

Author:: Eugenia Naro‐Maciel; Kristen M. Hart; Rossana Cruciata; Nathan F. Putman
Source:: Ecography 2017 v.40 no.5 pp. 586-597
ISSN:: 0906-7590
Subject:: Chelonia mydas; DNA; adults; breeding; coasts; courtship; evolution; genetic analysis; genetic similarity; genetic variation; juveniles; males; migratory behavior; mitochondria; mitochondrial genome; models; national parks; philopatry; population structure; turtles; water currents; wildlife management; Florida
Abstract:: ... Population structure and spatial distribution are fundamentally important fields within ecology, evolution, and conservation biology. To investigate pan‐Atlantic connectivity of globally endangered green turtles Chelonia mydas from two National Parks in Florida, USA, we applied a multidisciplinary approach comparing genetic analysis and ocean circulation modeling. The Everglades (EP) is a juvenile ...
DOI:: 10.1111/ecog.02056
: https://doi.org/10.1111/ecog.02056

82. Development and evaluation of the Axiom® IStraw35 384HT array for the allo-octoploid cultivated strawberry Fragaria ×ananassa

Author:: S. Verma; N. V. Bassil; E. van de Weg; R. J. Harrison; A. Monfort; J. M. Hidalgo; I. Amaya; B. Denoyes; L. Mahoney; T. M. Davis; Z. Fan; S. Knapp; V. M. Whitaker
Source:: Acta horticulturae 2017 v. no.1156 pp. 75-82
ISSN:: 0567-7572
Subject:: DNA; Fragaria ananassa; breeding programs; genome; genotyping; prices; research programs; single nucleotide polymorphism; strawberries; Florida
Abstract:: ... The Axiom® IStraw90 SNP (single nucleotide polymorphism) array was developed to enable high-throughput genotyping in allo-octoploid cultivated strawberry (Fragaria ×ananassa). However, high cost ($80-105 sample-1) limits throughput for certain applications. On average the IStraw90 has yielded 50 to 60% usable data points, defined as PHR (Poly High Resolution) and NMH (No Minor Homozygote) marker c ...
DOI:: 10.17660/ActaHortic.2017.1156.10
: https://doi.org/10.17660/ActaHortic.2017.1156.10

83. Effects of Florida Red Tides on histone variant expression and DNA methylation in the Eastern oyster Crassostrea virginica

Author:: Rodrigo Gonzalez-Romero; Victoria Suarez-Ulloa; Javier Rodriguez-Casariego; Daniel Garcia-Souto; Gabriel Diaz; Abraham Smith; Juan Jose Pasantes; Gary Rand; Jose M. Eirin-Lopez
Source:: Aquatic toxicology 2017 v.186 pp. 196-204
ISSN:: 0166-445X
Subject:: Crassostrea virginica; DNA; DNA damage; DNA methylation; Karenia brevis; algae; algal blooms; aquatic invertebrates; brevetoxins; coasts; conservation programs; epigenetics; gene expression; genes; genetic markers; genotoxicity; histones; mortality; oysters; phenotype; phosphorylation; Florida
Abstract:: ... Massive algal proliferations known as Harmful Algal Blooms (HABs) represent one of the most important threats to coastal areas. Among them, the so-called Florida Red Tides (FRTs, caused by blooms of the dinoflagellate Karenia brevis and associated brevetoxins) are particularly detrimental in the southeastern U.S., causing high mortality rates and annual losses in excess of $40 million. The ability ...
DOI:: 10.1016/j.aquatox.2017.03.006
: https://doi.org/10.1016/j.aquatox.2017.03.006

84. First report of Phyllosticta citricarpa and description of two new species, P. paracapitalensis and P. paracitricarpa, from citrus in Europe

Author:: V. Guarnaccia; J.Z. Groenewald; H. Li; C. Glienke; E. Carstens; V. Hattingh; P.H. Fourie; P.W. Crous
Source:: Studies in mycology 2017 v.87 pp. 161-185
ISSN:: 0166-0616
Subject:: Citrus; DNA; Guignardia citricarpa; Phyllosticta; citrus black spot; climate; crop production; data collection; endophytes; fruits; fungi; gardens; genes; hosts; internal transcribed spacers; leaves; necrosis; new species; oranges; orchards; plant collections; plant litter; rain; saprophytes; summer; surveys; Australia; China; Florida; Greece; Italy; Malta; Portugal; South Africa; South America; Spain
Abstract:: ... The genus Phyllosticta occurs worldwide, and contains numerous plant pathogenic, endophytic and saprobic species. Phyllosticta citricarpa is the causal agent of Citrus Black Spot disease (CBS), affecting fruits and leaves of several citrus hosts (Rutaceae), and can also be isolated from asymptomatic citrus tissues. Citrus Black Spot occurs in citrus-growing regions with warm summer rainfall climat ...
DOI:: 10.1016/j.simyco.2017.05.003
PubMed:: 28720979
PubMed Central:: PMC5502700
: https://doi.org/10.1016/j.simyco.2017.05.003

85. Occurrence of a novel mastrevirus in sugarcane germplasm collections in Florida, Guadeloupe and Réunion

Author:: Wardatou Boukari; Ricardo I. Alcalá-Briseño; Simona Kraberger; Emmanuel Fernandez; Denis Filloux; Jean-Heinrich Daugrois; Jack C. Comstock; Jean-Michel Lett; Darren P. Martin; Arvind Varsani; Philippe Roumagnac; Jane E. Polston; Philippe C. Rott
Source:: Virology journal 2017 v.14 no.1 pp. 146
ISSN:: 1743-422X
Subject:: DNA; Mastrevirus; Saccharum officinarum; amino acid sequences; genome; germplasm; germplasm conservation; islands; metagenomics; molecular cloning; nucleotide sequences; phylogeny; sequence analysis; sugarcane; viruses; Barbados; China; Florida; Guadeloupe; Reunion
Abstract:: ... BACKGROUND: In Africa and Asia, sugarcane is the host of at least seven different virus species in the genus Mastrevirus of the family Geminiviridae. However, with the exception of Sugarcane white streak virus in Barbados, no other sugarcane-infecting mastrevirus has been reported in the New World. Conservation and exchange of sugarcane germplasm using stalk cuttings facilitates the spread of suga ...
DOI:: 10.1186/s12985-017-0810-9
PubMed:: 28754134
PubMed Central:: PMC5534050
: https://doi.org/10.1186/s12985-017-0810-9

86. Phylogeography of the reef‐building polychaetes of the genus Phragmatopoma in the western Atlantic Region

Author:: Flavia L. D. Nunes; Alain Van Wormhoudt; Larisse Faroni‐Perez; Jérôme Fournier
Source:: Journal of biogeography 2017 v.44 no.7 pp. 1612-1625
ISSN:: 0305-0270
Subject:: Bayesian theory; DNA; Polychaeta; coasts; environmental factors; genetic variation; internal transcribed spacers; larvae; littoral zone; loci; mitochondria; models; phylogeny; phylogeography; population growth; population structure; reefs; Atlantic Ocean; Brazil; Caribbean; Florida
Abstract:: ... AIM: To verify the synonymy of the reef‐building polychaete Phragmatopoma caudata (described for the Caribbean) and Phragmatopoma lapidosa (described for Brazil) using molecular data. To evaluate the patterns of genetic diversity and connectivity among populations from Florida to South Brazil. LOCATION: Intertidal zone in the western Atlantic biogeographical Region: Brazil, eastern Caribbean and F ...
DOI:: 10.1111/jbi.12938
: https://doi.org/10.1111/jbi.12938

87. Potential Noncutaneous Sites of Chelonid Herpesvirus 5 Persistence and Shedding in Green Sea Turtles Chelonia mydas

Author:: Annie Page-Karjian; Nicole L. Gottdenker; Jordyn Whitfield; Lawrence Herbst; Terry M. Norton; Branson Ritchie
Source:: Journal of Aquatic Animal Health 2017 v.29 no.3 pp. 136-142
ISSN:: 1548-8667
Subject:: quantitative polymerase chain reaction; Chelonia mydas; sea turtles; urine; etiological agents; virion; neoplasms; DNA; Chelonid alphaherpesvirus 5; nerve tissue; kidneys; keratinocytes; heart; virus replication; juveniles; Florida
Abstract:: ... Chelonid herpesvirus 5 (ChHV5), the likely etiologic agent of sea turtle fibropapillomatosis (FP), is predicted to be unevenly distributed within an infected turtle, in which productive virus replication and virion shedding occurs in cutaneous tumor keratinocytes. In this study, we measured and compared ChHV5 DNA quantities in tumors, skin, urine, major organs, and nervous tissue samples from gree ...
DOI:: 10.1080/08997659.2017.1321590
: https://doi.org/10.1080/08997659.2017.1321590

88. Sugar cane processing residue, bagasse, enhances decomposition of citrus leaves and could contribute to citrus black spot management

Author:: Ariena H.C. van Bruggen; Kalpana Sharma; Keumchul Shin
Source:: Crop protection 2017 v.93 pp. 89-97
ISSN:: 0261-2194
Subject:: Citrus; DNA; Guignardia citricarpa; bagasse; branches; citrus black spot; field experimentation; groves; leaves; microorganisms; oranges; pathogens; plant litter; quantitative polymerase chain reaction; quarantine; sprinklers; sugarcane; urea; water content; weight loss; Florida
Abstract:: ... Citrus black spot (CBS) is a quarantine disease, reported in Florida in 2010. The pathogen, Phyllosticta citricarpa (Pc), survives in twigs and leaf litter during the off-season. Speeding up leaf litter decomposition could contribute to reducing disease pressure and potential spread. Objectives were to investigate (a) effects of bagasse, cellulolytic microbes and urea on leaf litter decomposition; ...
DOI:: 10.1016/j.cropro.2016.11.022
: https://doi.org/10.1016/j.cropro.2016.11.022

89. Tracing the origin of a cryptic invader: phylogeography of the Euwallacea fornicatus (Coleoptera: Curculionidae: Scolytinae) species complex

Author:: Richard Stouthamer; Paul Rugman‐Jones; Pham Q. Thu; Akif Eskalen; Tim Thibault; Jiri Hulcr; Liang‐Jong Wang; Bjarte H. Jordal; Chi‐Yu Chen; Miriam Cooperband; Ching‐Shan Lin; Naoto Kamata; Sheng‐Shan Lu; Hayato Masuya; Zvi Mendel; Robert Rabaglia; Sunisa Sanguansub; Hsin‐Hui Shih; Wisut Sittichaya; Shixiang Zong
Source:: Agricultural and forest entomology 2017 v.19 no.4 pp. 366-375
ISSN:: 1461-9555
Subject:: Curculionidae; DNA; Euwallacea fornicatus; ambrosia beetles; avocados; biological control; cryptic species; mitochondrial genes; natural enemies; nucleotide sequences; phylogeography; plant pests; trees; California; Florida; Hawaii
Abstract:: ... The ambrosia beetle morphologically identified as Euwallacea fornicatus consists of several cryptic species that exhibit large differences in the DNA sequences of several nuclear and mitochondrial gene regions. Based on these differences, we suggest that there are at least three different species each with distinct phylogeography. Members of this cryptic species complex have invaded many areas out ...
DOI:: 10.1111/afe.12215
: https://doi.org/10.1111/afe.12215

90. A Key to Some Frankliniella (Thysanoptera: Thripidae) Larvae Found in Florida with Descriptions of the First Instar of Select species

Author:: Skarlinsky Thomas; Funderburk Joe
Source:: Florida entomologist 2016 v.99 no.3 pp. 463-470
ISSN:: 0015-4040
Subject:: DNA; Frankliniella; adults; genes; host plants; instars; larvae; mitochondria; polymerase chain reaction; researchers; sequence analysis; taxonomic keys; Florida
Abstract:: ... Few publications exist toward the identification of larval thrips. As a result, researchers and practitioners often are unable to report larval species data or may misinterpret what is a host plant when adults of multiple species are collected. Therefore, we conducted repetitive plant sampling and detailed examination of larvae with adults, which revealed morphological differences of some undescri ...
DOI:: 10.1653/024.099.0319
: https://doi.org/10.1653/024.099.0319

91. Combining Tpi and CO1 Genetic Markers to Discriminate Invasive Helicoverpa armigera From Local Helicoverpa zea (Lepidoptera: Noctuidae) Populations in the Southeastern United States

Author:: Rodney N. Nagoshi; Todd M. Gilligan; Julieta Brambila
Source:: Journal of economic entomology 2016 v.109 no.5 pp. 2115-2124
ISSN:: 0022-0493
Subject:: DNA; Helicoverpa armigera; Helicoverpa zea; cytochrome-c oxidase; genes; genetic markers; genetic variation; hybridization; hybrids; insect infestations; insect pests; invasive species; mitochondria; monitoring; nucleotide sequences; pesticides; phylogeny; risk; sibling species; triose-phosphate isomerase; Australia; Europe; Florida; Morocco; South America
Abstract:: ... The recent establishment of the Old World pest Helicoverpa armigera (Hübner) into South America has had significant economic consequences and places the rest of the hemisphere at risk, emphasizing the need for improved methods of monitoring. A major complication is that a sibling species endemic to the New World, Helicoverpa zea (Boddie), is morphologically very similar, with the two species capab ...
DOI:: 10.1093/jee/tow177
: https://doi.org/10.1093/jee/tow177

92. Genotyping Sugarcane for the Brown Rust Resistance Locus Bru1 Using Unlabeled Probe Melting

Author:: Per H. McCord; Andrew J. Migneault
Source:: Sugar tech 2016 v.18 no.4 pp. 401-406
ISSN:: 0972-1525
Subject:: DNA; Puccinia melanocephala; Saccharum; USDA; cost analysis; dissociation; dyes; fluorescence; gel electrophoresis; genetic resistance; genotype; genotyping; hybrids; leaf rust; loci; melting; oligonucleotides; plant pathogenic fungi; polymerase chain reaction; restriction endonucleases; risk; single nucleotide polymorphism; sugarcane; Florida; Louisiana
Abstract:: ... Brown rust, caused by the fungus Puccinia melanocephala, is a major disease of sugarcane (Saccharum spp. hybrid) in Florida, Louisiana, and other sugarcane growing regions. The Bru1 locus has been used as a durable and effective source of resistance, and markers are available to select for the trait. The markers currently being used by the USDA Sugarcane Field Station in Canal Point, FL for Bru1 g ...
DOI:: 10.1007/s12355-015-0390-1
: https://doi.org/10.1007/s12355-015-0390-1

93. Occurrence of 16SrIV Subgroup A Phytoplasmas in Roystonea regia and Acrocomia mexicana Palms with Lethal Yellowing‐like Syndromes in Yucatán, Mexico

Author:: María Narvaez; Iván Córdova‐Lara; Celso Reyes‐Martínez; Carlos Puch‐Hau; Luis Mota‐Narvaez; Ana Collí; Goretti Caamal; Nigel Harrison; Luis Sáenz; Carlos Oropeza
Source:: Phytopathologische Zeitschrift 2016 v.164 no.11-12 pp. 1111-1115
ISSN:: 0931-1785
Subject:: Acrocomia aculeata; DNA; Phytoplasma; Roystonea regia; atrophy; inflorescences; leaves; lethal yellowing; necrosis; polymerase chain reaction; restriction fragment length polymorphism; Florida; Mexico; South America
Abstract:: ... The lethal yellowing (LY) disease and LY‐type syndromes affecting several palm species are associated with 16SrIV phytoplasmas in the Americas. In Mexico, palms of the species Roystonea regia and the native Acrocomia mexicana were found to exhibit LY‐type symptoms, including leaf decay, starting with mature leaves, necrosis and atrophy of inflorescences. DNA extracts obtained from these palms coul ...
DOI:: 10.1111/jph.12464
: https://doi.org/10.1111/jph.12464

94. Plum selections with total resistance to leaf scald (Xylella fastidiosa)

Author:: M. A. Dalbo; E. D. Bruna; R. O. Nodari; L. Saifert
Source:: Acta horticulturae 2016 no.1127 pp. 61-64
ISSN:: 0567-7572
Subject:: Cicadellidae; DNA; Xylella fastidiosa; alternative hosts; bacteria; breeding programs; crossing; cultivars; genotype; mature plants; parents; petioles; plums; polymerase chain reaction; Brazil; Florida
Abstract:: ... Leaf scald (Xylella fastidiosa) is the most important plum disease in Brazil. It is widespread in the main production areas because of the presence of effective vectors (sharpshooters) and alternative hosts. Most plum cultivars are susceptible, and the main challenge of plum breeding in Brazil is to obtain resistant cultivars. An extensive breeding program has been developed since 1990 in Santa Ca ...
DOI:: 10.17660/ActaHortic.2016.1127.11
: https://doi.org/10.17660/ActaHortic.2016.1127.11

95. Prevalence of Tick-Borne Pathogens in Host-Seeking Amblyomma americanum (Acari: Ixodidae) and Odocoileus virginianus (Artiodactyla: Cervidae) in Florida

Author:: Katherine A. Sayler; Amanda D. Loftis; Sarah K. Beatty; Carisa L. Boyce; Elina Garrison; Bambi Clemons; Mark Cunningham; Arthur R. Alleman; Anthony F. Barbet
Source:: Journal of medical entomology 2016 v.53 no.4 pp. 949-956
ISSN:: 0022-2585
Subject:: Amblyomma americanum; Borrelia burgdorferi; Borrelia lonestari; DNA; Ehrlichia chaffeensis; Ehrlichia ewingii; Odocoileus virginianus; Rickettsia; antibodies; fever; geographical distribution; host seeking; humans; microorganisms; pathogenicity; pathogens; polymerase chain reaction; sequence analysis; serology; state parks; ticks; zoonoses; Florida
Abstract:: ... Amblyomma americanum (L.), the lone star tick, is an aggressive tick that is expanding its geographic range within the United States. This tick is the vector for the human and veterinary pathogens Ehrlichia chaffeensis and Ehrlichia ewingii and is associated with other microbes of unspecified pathogenicity including Rickettsia amblyommii, Panola Mountain Ehrlichia, and Borrelia lonestari. In Flori ...
DOI:: 10.1093/jme/tjw054
: https://doi.org/10.1093/jme/tjw054

96. Using droplet digital PCR (ddPCR) to detect copy number variation in sugarcane, a high-level polyploid

Author:: Per H. McCord
Source:: Euphytica 2016 v.209 no.2 pp. 439-448
ISSN:: 0014-2336
Subject:: DNA; Saccharum; alleles; clones; dose response; droplets; fungi; gene expression; germplasm; leaf rust; loci; molecular biology; parents; phenotype; plant breeding; polyploidy; progeny; quantitative polymerase chain reaction; sugarcane; testcrosses; Florida
Abstract:: ... Copy number variation (CNV) generally refers to duplications or deletions of sections of DNA, including genes. In polyploid plant species, CNV can also take the form of multiple copies of an allele at a given locus. This variation in dosage has been shown to alter gene expression and phenotype. As copy number rises, it becomes increasingly challenging to quantify dosage changes. A new technique, k ...
DOI:: 10.1007/s10681-016-1657-7
: https://doi.org/10.1007/s10681-016-1657-7

97. Genetic Population Structure of Black Drum in U.S. Waters

Author:: Jacqueline M. Leidig; Virginia R. Shervette; Christopher J. McDonough; Tanya L. Darden
Source:: North American Journal of Fisheries Management 2015 v.35 no.3 pp. 464-477
ISSN:: 1548-8675
Subject:: Cynoscion nebulosus; DNA; Pogonias cromis; Sciaenops ocellatus; coasts; fish; fisheries; gene flow; genetic markers; genetic variation; genotype; life history; loci; microsatellite repeats; population structure; Delaware; Florida; Gulf of Mexico; North Carolina; Virginia
Abstract:: ... The Black Drum Pogonias cromis is an estuarine-dependent fish that supports recreational and commercial fisheries throughout its range along the U.S. Atlantic coast and Gulf of Mexico (GOM) coast. We used nuclear microsatellite markers and samples collected from multiple locations along both the U.S. Atlantic and GOM coasts to evaluate the stock structure of Black Drum and to examine small-scale s ...
DOI:: 10.1080/02755947.2015.1017123
: https://doi.org/10.1080/02755947.2015.1017123

98. Genomewide investigation of adaptation to harmful algal blooms in common bottlenose dolphins (Tursiops truncatus)

Author:: Kristina M. Cammen; Thomas F. Schultz; Patricia E. Rosel; Randall S. Wells; Andrew J. Read
Source:: Molecular ecology 2015 v.24 no.18 pp. 4697-4710
ISSN:: 0962-1083
Subject:: DNA; Karenia brevis; Tursiops truncatus; algal blooms; brevetoxins; coasts; dolphins; epidemiology; etiology; gene frequency; genes; genomics; high-throughput nucleotide sequencing; humans; loci; mortality; neurotoxicity; resistance mechanisms; single nucleotide polymorphism; trophic relationships; Florida; Gulf of Mexico
Abstract:: ... Harmful algal blooms (HABs), which can be lethal in marine species and cause illness in humans, are increasing worldwide. In the Gulf of Mexico, HABs of Karenia brevis produce neurotoxic brevetoxins that cause large‐scale marine mortality events. The long history of such blooms, combined with the potentially severe effects of exposure, may have produced a strong selective pressure for evolved resi ...
DOI:: 10.1111/mec.13350
: https://doi.org/10.1111/mec.13350

99. High prevalence of Trichinella pseudospiralis in Florida panthers (Puma concolor coryi)

Author:: Mason V Reichard; Marc Criffield; Jennifer E Thomas; Jacqueline M Paritte; Mark Cunningham; Dave Onorato; Kenneth Logan; Maria Interisano; Gianluca Marucci; Edoardo Pozio
Source:: Parasites & vectors 2015 v.8 no.1 pp. 67
ISSN:: 1756-3305
Subject:: DNA; Puma concolor; Sus scrofa; Trichinella pseudospiralis; birds; carnivores; cats; diet; digestion; genotype; larvae; mixed infection; necropsy; parasites; polymerase chain reaction; swine; Florida
Abstract:: ... BACKGROUND: Parasites of the genus Trichinella are zoonotic nematodes common in carnivores throughout the world. We determined the prevalence and species of Trichinella infections in Florida panthers (Puma concolor coryi). METHODS: Tongues from Florida panthers were collected at necropsy and examined by pepsin-HCl artificial digestion for infection with Trichinella spp. DNA was extracted from larv ...
DOI:: 10.1186/s13071-015-0674-z
PubMed:: 25649739
PubMed Central:: PMC4324651
: https://doi.org/10.1186/s13071-015-0674-z

100. Molecular surveillance of plant viruses: identification of new and emerging viruses of tomato before they cause epidemics

Author:: J. E. Polston; K. Rosario; M. Breitbart
Source:: Acta horticulturae 2015 no.1069 pp. 127-131
ISSN:: 0567-7572
Subject:: plant viruses; Carlavirus; genome; Orthotospovirus; insects; adults; tomatoes; Begomovirus; Solanum lycopersicum; DNA; Potexvirus; vegetable crops; Crinivirus; viruses; Torradovirus; Bemisia tabaci; monitoring; high-throughput nucleotide sequencing; Ipomovirus; disease outbreaks; metagenomics; RNA; Florida
Abstract:: ... Tomato (Solanum lycopersicum) is a crop with a large number of viral pathogens (estimated at 136 in 2010). Therefore it is not surprising that viruses of tomato continue to emerge, either as new pathogens or as known pathogens that have appeared in new areas or have increased in incidence. Emerging viruses of tomato are found in a diverse array of genera: Annulavirus, Begomovirus, Crinivirus, Pote ...

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1. Contextualizing enigmatic extinctions using genomic DNA from fluid-preserved museum specimens of Desmognathus salamanders

2. First Report of Botrytis cinerea Causing Leaf Spot on Strawberry in Florida

3. First Report of Meloidogyne javanica Pathogenic on Hybrid Lavender (Lavandula × intermedia) in the United States

4. First Report of Neopestalotiopsis clavispora Causing Calyx and Receptacle Blight on Strawberry in China

5. Genome-wide assessment of population structure in Florida’s coastal seaside sparrows

6. Review of the genus Palmicultor Williams, 1963 (Hemiptera: Coccomorpha: Pseudococcidae), with evidence of a cryptic species

7. Absence of Herpesvirus in a Survey of Alligator Snapping Turtles (Macrochelys temminckii) in Illinois and Louisiana

8. Caulerpa wysorii sp. nov., a denuded Caulerpa (Chlorophyta) resembling C. sertularioides when ‘dressed’

9. Development of a Multiplex High-Throughput Diagnostic Assay for the Detection of Strawberry Crown Rot Diseases Using High-Resolution Melting Analysis

10. Development of a simple one-step multiplex RT-PCR system for simultaneous detection of DNA and RNA viruses of cucurbit leaf crumple virus, cucurbit yellow stunting disorder virus, squash vein yellowing virus, and cucurbit chlorotic yellows virus

11. Esteya floridanum sp. nov.: An Ophiostomatalean Nematophagous Fungus and Its Potential to Control the Pine Wood Nematode

12. First Report of Alternaria spp. Causing Leaf Spot on Sweet Viburnum in China

13. First Report of Bipolaris yamadae Leaf Spot Disease on Guinea Grass (Panicum maximum) in Florida

14. First Report of Diaporthe phaseolorum Causing Stem Canker of Hemp (Cannabis sativa)

15. First Report of Neopestalotiopsis rosae Causing Foliar and Fruit Spots on Pomegranate in Florida

16. First Report of Neoscytalidium dimidiatum Causing Stem Canker on Dragon fruit (Hylocereus spp.) in Puerto Rico

17. Incidence of Asiatic Citrus Canker on Trifoliate Orange and Its Hybrid Accessions in a Florida Field Planting

18. Insights on Immune Function in Free-Ranging Green Sea Turtles (Chelonia mydas) with and without Fibropapillomatosis

19. Phosphorus retention within a relic agricultural ditch in a constructed wetland

20. Reptile Host Associations of Ixodes scapularis in Florida and Implications for Borrelia spp. Ecology

21. Taxonomic and identification review of adventive Fiorinia Targioni Tozzetti (Hemiptera, Coccomorpha, Diaspididae) of the United States

22. Use of ITS-1 to Identify Bactrocera dorsalis and Bactrocera occipitalis (Diptera: Tephritidae): A Case Study Using Flies Trapped in California from 2008 to 2018

23. Using molecular gut content analysis to identify key predators in a classical weed biological control system: a study with Neomusotima conspurcatalis (Lepidoptera: Crambidae)

24. A multi‐state occupancy modelling framework for robust estimation of disease prevalence in multi‐tissue disease systems

25. Analysis of Trace Element Concentrations and Antioxidant Enzyme Activity in Muscle Tissue of the Atlantic Sharpnose Shark, Rhizoprionodon terraenovae

26. Bivalve Feeding Responses to Microalgal Bloom Species in the Indian River Lagoon: the Potential for Top-Down Control

27. Coloration Patterns of the Tegmina of Mahanarva Spectabilis (Hemiptera: Cercopidae): Biological, Morphological and Genetic Bases

28. DNA sequencing and anatomy demonstrate that Pacific Codium simulans is a genetically variable species found in the floras of Bermuda and Florida

29. Detection of Cercopithifilaria bainae infection in shelter dogs and ticks in Oklahoma, USA

30. Dispersal and Land Cover Contribute to Pseudorabies Virus Exposure in Invasive Wild Pigs

31. First Report of Curvularia pseudobrachyspora Causing Leaf Spot on Hemp (Cannabis sativa) in Florida

32. First Report of Fruit Rot of Macadamia sp. Caused by Phytophthora heveae in Yunnan, China

33. First Report of Fusarium oxysporum f. sp. lactucae Causing Vascular Wilt of Lettuce in Florida

34. First Report of Leaf Spot of Alcea rosea Caused by Alternaria alternata in Italy

35. First Report of Poinsettia Wilt Caused by Amphobotrys ricini (syn. Botryotinia ricini) in Florida

36. First Report of Sclerotinia sclerotiorum Causing Strawberry Fruit Rot in Florida

37. Haplaxius crudus (Hemiptera: Cixiidae) Transmits the Lethal Yellowing Phytoplasmas, 16SrIV, to Pritchardia pacifica Seem. & H.Wendl (Arecaceae) in Yucatan, Mexico

38. Implementing Low-Cost, High Accuracy DNA Barcoding From Single Molecule Sequencing to Screen Larval Tephritid Fruit Flies Intercepted at Ports of Entry

39. Morphological, Molecular, and Biogeographic Evidence for Specific Recognition of Euthamia hirtipes and Euthamia scabra (Asteraceae, Astereae)

40. Pathogen Surveillance and Detection of Ranavirus (Frog virus 3) in Translocated Gopher Tortoises (Gopherus polyphemus)

41. Revealing the Diet of Generalist Insect Predators in Strawberry Fields: Not Only Pests, But Other Predators Beware

42. A Survey of Tick-Borne Bacterial Pathogens in Florida

43. Culicoides species community composition and infection status with parasites in an urban environment of east central Texas, USA

44. Efficacy of eDNA as an early detection indicator for Burmese pythons in the ARM Loxahatchee National Wildlife Refuge in the Greater Everglades Ecosystem

45. Evaluating the taxonomic status of the Great White Heron (Ardea herodias occidentalis) using morphological, behavioral and genetic evidence

46. First Report of Crown Rot Caused by Phytopythium helicoides on Strawberry in the Americas

47. First Report of Erysiphe fallax Causing Powdery Mildew on Phasey Bean (Macroptilium lathyroides) in the United States

48. First Report of Lasiodiplodia theobromae and Neofusicoccum parvum/N. ribis species Complex Causing Fruit Rot on Strawberry in the United States

49. First Report of Southern Root-Knot Nematode, Meloidogyne incognita, on Large Crabgrass, Digitaria sanguinalis, in China

50. Genetic Variation among Island and Continental Populations of Burrowing Owl (Athene cunicularia) Subspecies in North America

51. Melanothamnus maniticola sp. nov. (Ceramiales, Rhodophyta): an epizoic species evolved for living on the West Indian Manatee

52. Mycotic Rhinitis and Sinusitis in Florida Horses

53. Patterns of genetic divergence among populations of Aedes aegypti L. (Diptera: Culicidae) in the southeastern USA

54. Pilot Study on the Effect of Cocaine Use on the Intestinal Microbiome and Metabolome and Inflammation in HIV-Infected Adults in the Miami Adult Studies in HIV (MASH) Cohort (P13-027-19)

55. Survey of Texas Bay Systems for the Oyster Parasites Bonamia spp., Haplosporidium nelsoni, and Perkinsus marinus

56. Temporal patterns of entomopathogenic nematodes in Florida citrus orchards: Evidence of natural regulation by microorganisms and nematode competitors

57. Using Noninvasive Genetic Sampling to Survey Rare Butterfly Populations

58. Evaluation of the novel crAssphage marker for sewage pollution tracking in storm drain outfalls in Tampa, Florida

59. Evidence of pseudorabies virus shedding in feral swine (sus scrofa) populations of florida, usa

60. First Report of Alternaria Leaf Spot of Cilantro (Coriandrum sativum) Caused by Alternaria dauci in the United States

61. First Report of Diplodia sapinea and Diplodia scrobiculata Causing an Outbreak of Tip Blight on Slash Pine in Florida

62. First Report of Phytophthora nicotianae Causing Crown Rot of Strawberry in the United States

63. First Report of Rust Caused by Puccinia arachidis on Peanut in South Carolina

64. First Report of Tar Spot on Corn (Zea mays) Caused by Phyllachora maydis in Florida, Iowa, Michigan, and Wisconsin

65. First Report of the Root Lesion Nematode, Pratylenchus bolivianus, on Aspalathus linearis in South Africa

66. Histologic characterization of eosinophilic encephalitis in horses in Florida

67. Necrotizing interstitial pneumonia and suppurative myocarditis associated with Bartonella henselae infection in three Florida pumas

68. Pyroaerobiology: the aerosolization and transport of viable microbial life by wildland fire

69. Screwworm (Diptera: Calliphoridae) in the United States: Response to and Elimination of the 2016–2017 Outbreak in Florida

70. Serological and molecular prevalence of equine piroplasmosis in Western Java, Indonesia