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1. Collaborative trial to assess the performance of digital PCR in the field of GMO analysis using an artificial sample material

Author:
Moor, Dominik,   et al.   ; Köppel, René; Peterseil, Verena; Dagand, Emilie; Schütz, Ekkehard; Kolberg, Nina; Milavec, Mojca;     Show all 7 Authors
Source:
European food research & technology 2017 v.243 no.6 pp. 1091-1096
ISSN:
1438-2377
Subject:
DNA; chemical elements; equipment; genetically modified organisms; lectins; polymerase chain reaction; transgenes
Abstract:
... Digital PCR promises absolute quantification of amplifiable nucleic acids molecules. In consequence, results should be comparable, irrespective of the analysing laboratory and the equipment used. To assess this claim, we used a multitarget DNA molecule (amplicon) encoding for eight transgene soy traits and the lectin soy reference gene. This multitarget amplicon was analysed by seven European labo ...
DOI:
10.1007/s00217-016-2824-8

2. Real-time PCR method for the detection of figwort mosaic virus (FMV) to complement the FMV 34S promoter-specific PCR assay used for screening of genetically modified plants

Author:
Moor, Dominik,   et al.   ; Liniger, Marianne; Grohmann, Lutz; Felleisen, Richard;     Show all 4 Authors
Source:
European food research & technology 2012 v.235 no.5 pp. 835-842
ISSN:
1438-2377
Subject:
DNA; Figwort mosaic virus; cross reaction; detection limit; equipment; nontarget organisms; open reading frames; plant viruses; polymerase chain reaction; screening; transgenic plants
Abstract:
... In this study a new real-time PCR assay for the detection of figwort mosaic virus (FMV) DNA is described. This assay targets a 113-bp-long sequence of the FMV open reading frame Vll, a non-conserved coding region among the caulimoviruses. Detection of FMV DNA is useful to complement screening for the FMV 34S promoter (P-FMV), a genetic element present in several genetically modified (GM) plants. T ...
DOI:
10.1007/s00217-012-1811-y