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1. Multiplex real-time PCR Method for the detection and quantification of DNA from the four transgenic soy traits DP-356043-5, DP-305423-1, MON 87701, and BPS-CV127-9 and lectin

Author:
Moor, Dominik,   et al.   ; Köppel, René; Bucher, Thomas; Meuwly, Alain;     Show all 4 Authors
Source:
European food research & technology 2014 v.239 no.2 pp. 347-355
ISSN:
1438-2377
Subject:
DNA; crops; lectins; quantitative polymerase chain reaction; soybeans; transgenic plants
Abstract:
... According to the release of authorization, several new transgenic soy crops are expected to be present in the harvest of soy beans. In contrast to earlier times, methods for the detection of these transgenic crops are available already in advance. However, these methods are mainly still inefficient single real-time PCR methods. To increase the efficiency of product control, a multiplex quantitativ ...
DOI:
10.1007/s00217-014-2229-5

2. Collaborative trial to assess the performance of digital PCR in the field of GMO analysis using an artificial sample material

Author:
Moor, Dominik,   et al.   ; Köppel, René; Peterseil, Verena; Dagand, Emilie; Schütz, Ekkehard; Kolberg, Nina; Milavec, Mojca;     Show all 7 Authors
Source:
European food research & technology 2017 v.243 no.6 pp. 1091-1096
ISSN:
1438-2377
Subject:
DNA; chemical elements; equipment; genetically modified organisms; lectins; polymerase chain reaction; transgenes
Abstract:
... Digital PCR promises absolute quantification of amplifiable nucleic acids molecules. In consequence, results should be comparable, irrespective of the analysing laboratory and the equipment used. To assess this claim, we used a multitarget DNA molecule (amplicon) encoding for eight transgene soy traits and the lectin soy reference gene. This multitarget amplicon was analysed by seven European labo ...
DOI:
10.1007/s00217-016-2824-8