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1. 1-Methylcyclopropene (1-MCP) treatment differentially mediated expression of vacuolar processing enzyme (VPE) genes and delayed programmed cell death (PCD) during ripening and senescence of apple fruit

Author:
Jingyi Lv; Yingzhi Zhang; Mingyu Sun; Jingxin Chen; Yonghong Ge; Jianrong Li
Source:
Scientia horticulturae 2023 v.307 pp. 111489
ISSN:
0304-4238
Subject:
1-methylcyclopropene; DNA; Malus domestica; apples; climacteric fruits; electrical conductivity; enzymes; ethylene; firmness; malondialdehyde; programmed cell death; reactive oxygen species; transmission electron microscopy; vacuoles
Abstract:
... The main purpose of this research was to evaluate the impacts of 1-methylcyclopropene (1-MCP) treatment on vacuolar processing enzyme (VPE) genes and programmed cell death (PCD) during ripening and senescence of apple fruit (Malus × domestica Borkh.). The ethylene antagonists 1-MCP (1 μL L⁻¹) was applied to apples after harvest. Both treated and control fruit were stored at 20 ± 2°C. The data show ...
DOI:
10.1016/j.scienta.2022.111489

2. Ammonia-induced oxidative stress triggered proinflammatory response and apoptosis in pig lungs

Author:
Daojie Li; Long Shen; Di Zhang; Xiaotong Wang; Qiankun Wang; Wenhao Qin; Yun Gao; Xiaoping Li
Source:
Journal of environmental sciences (China) 2023 v.126 pp. 683-696
ISSN:
1001-0742
Subject:
DNA; Western blotting; ammonia; animal models; apoptosis; dose response; epithelium; genes; inflammation; lungs; metabolome; mineral fertilizers; oxidative phosphorylation; oxidative stress; protein synthesis; pyruvic acid; swine; toxicity; transcriptome; transmission electron microscopy; China
Abstract:
... Ammonia, a common toxic gas, is not only one of the main causes of haze, but also can enter respiratory tract and directly affect the health of humans and animals. Pig was used as an animal model for exploring the molecular mechanism and dose effect of ammonia toxicity to lung. In this study, the apoptosis of type II alveolar epithelial cells was observed in high ammonia exposure group using trans ...
DOI:
10.1016/j.jes.2022.05.005

3. Anchoring red blood cell with tetrahedral DNA nanostructure: Electrochemical biosensor for the sensitive signage of circulating tumor DNA

Author:
Xinyue Zha; Weiwei Qin; Jiadi Chen; Mengting Chen; Qiyue Zhang; Kaiyu He; Yingju Liu; Weipeng Liu
Source:
Talanta 2023 v.251 pp. 123793
ISSN:
0039-9140
Subject:
DNA; biocompatibility; biosensors; blood serum; detection limit; erythrocytes; methylene blue; neoplasms; rapid methods
Abstract:
... Circulating tumor cells (CTCs), as a type of tumor, have attracted wide attention because of their characteristics of shedding from the primary tumor and spreading to other tissues and organs through peripheral blood. The circulating tumor DNA (ctDNA), the DNA released by CTCs and other tumor cells into the peripheral blood, was considered as a promising detection substance for clinical applicatio ...
DOI:
10.1016/j.talanta.2022.123793

4. Capsalid monogeneans of fishes from the Seto Inland Sea, Japan: Description of Benedenia kobudai n. sp. parasitic on Semicossyphus reticulatus (Perciformes: Labridae)

Author:
Masato Nitta
Source:
Parasitology international 2023 v.92 pp. 102677
ISSN:
1383-5769
Subject:
Capsalidae; DNA; Japan; Semicossyphus; aquaculture; biodiversity; fish; fisheries; host range; new species; parasitology; penis; phylogeny
Abstract:
... The Seto Inland Sea, the largest inland sea in Japan, is a rich fishery with high biodiversity and productivity. Monogeneans have been studied for more than 120 years, and 58 nominal species have been recorded in the Seto inland Sea. This study provided DNA information on five species of Benedenia sensu lato (Capsalidae) from marine fishes from the sea, and one of them, Benedenia kobudai n. sp., i ...
DOI:
10.1016/j.parint.2022.102677

5. Cascaded molecular logic gates using antibiotics as inputs based on exonuclease III and DNAzyme

Author:
Fang Deng; Jiafeng Pan; Zhi Liu; Junhua Chen
Source:
Talanta 2023 v.252 pp. 123832
ISSN:
0039-9140
Subject:
DNA; analytical methods; catalytic activity; chloramphenicol; fluorescence; kanamycin; nucleic acid hybridization; oxytetracycline
Abstract:
... We successfully constructed several cascaded molecular logic gates (2INHIBIT-2AND, 2AND-2OR, and 2OR-2INHIBIT) using three different antibiotics as the inputs. In the presence of kanamycin (KAN), chloramphenicol (CHL), or oxytetracycline (OXY), the aptamer-antibiotic recognition will release the trigger DNA to active the hairpin DNA hybridization. Exonuclease III (Exo III)-mediated catalysis react ...
DOI:
10.1016/j.talanta.2022.123832

6. Characterization and functional analysis of chicken dsRNA binding protein hnRNPU

Author:
Xifeng Hu; Xiangdong Wu; Zhen Ding; Zheng Chen; Huansheng Wu
Source:
Developmental and comparative immunology 2023 v.138 pp. 104521
ISSN:
0145-305X
Subject:
DNA; RNA; Vesiculovirus; chickens; cytosol; genomics; humans; immunology; nuclear matrix; ribonucleoproteins
Abstract:
... In mammals, heterogeneous ribonucleoprotein U (hnRNPU), also named as nuclear matrix protein-nuclear scaffold attachment factor (SAFA), was originally identified as a DNA/RNA interactor protein. It has been reported that human hnRNPU facilitates IFN-β generation after vesicular stomatitis virus (VSV) infection. Nevertheless, the role of chicken hnRNPU (chhnRNPU) in IFN-β regulation as well as in i ...
DOI:
10.1016/j.dci.2022.104521

7. Counting DNA molecules on a microchannel surface for quantitative analysis

Author:
Taesoo Kim; Siwon Kim; Chanyoung Noh; Hyeseung Hwang; Jieun Shin; Nabin Won; Seonghyun Lee; Dogeun Kim; Yoonha Jang; Su-Jin Hong; Jungyul Park; Sung Jae Kim; Seongsoo Jang; Kwang-Il Lim; Kyubong Jo
Source:
Talanta 2023 v.252 pp. 123826
ISSN:
0039-9140
Subject:
Escherichia coli; detection limit; fluorescence microscopy; gel electrophoresis; plasmids; quantitative analysis
Abstract:
... Microscopic visualization of DNA molecules is a simple, intuitive, and powerful method. Nonetheless, DNA-molecule quantification methods that employ microscopic visualization have not been reported so far. In this study, a new quantitative approach is presented that enables the counting of individual DNA molecules that have been rendered visible by fluorescence microscopy. Toward this, a microflui ...
DOI:
10.1016/j.talanta.2022.123826

8. Development and application of a multiplex PCR method to differentiate Salmonella enterica serovar Typhimurium from its monophasic variants in pig farms

Author:
Yaming Hong; Ruoyun Ji; Zhenyu Wang; Jiaojie Gu; Xinan Jiao; Qiuchun Li
Source:
Food microbiology 2023 v.109 pp. 104135
ISSN:
0740-0020
Subject:
DNA; Salmonella Typhimurium; detection limit; farms; flagellin; food microbiology; food production; genes; humans; polymerase chain reaction; rapid methods; salmonellosis; serotypes; swine
Abstract:
... Salmonella enterica serovar Typhimurium monophasic variants (Salmonella 4,[5],12:i:-) has increased dramatically, causing human salmonellosis and colonization in pigs. With a difference to S. Typhimurium, the monophasic variants of S. Typhimurium lose the gene cassettes encoding the second phase flagellin. To establish a rapid method to detect and differentiate the two serotypes, we analyzed the p ...
DOI:
10.1016/j.fm.2022.104135

9. Dietary fiber konjac glucomannan exerts an antidiabetic effect via inhibiting lipid absorption and regulation of PPAR-γ and gut microbiome

Author:
Muthukumaran Jayachandran; Sunil Christudas; Xuchu Zheng; Baojun Xu
Source:
Food chemistry 2023 v.403 pp. 134336
ISSN:
0308-8146
Subject:
absorption; blood lipids; body weight; dietary fiber; drugs; fibrosis; food chemistry; glycemic effect; high fat diet; hyperlipidemia; immunohistochemistry; insulin; intestinal microorganisms; konjac mannan; lipid composition; lipid metabolism; lipids; liver; microbiome; noninsulin-dependent diabetes mellitus; protein synthesis; streptozotocin
Abstract:
... Konjac glucomannan’s influence on the regulation of diabetes mellitus, hyperlipidemia, and gut microbial flora was evaluated in this study. In addition, a high-fat diet and streptozotocin were used to induce type 2 diabetes mellitus in rats. At the end of the study, we analyzed various parameters such as body weight, plasma lipid profile, insulin levels by immunohistochemistry, degree of fibrosis ...
DOI:
10.1016/j.foodchem.2022.134336

10. Dietary tryptophan requirement of fingerling Channa punctatus (Bloch) based on growth, hematological parameters, intestinal enzymes, non-specific immune response, and antioxidant capacity

Author:
Yusra Sharf; Mukhtar A. Khan
Source:
Aquaculture 2023 v.562 pp. 738745
ISSN:
0044-8486
Subject:
Channa punctata; DNA; RNA; alanine transaminase; antioxidant activity; aquaculture; aspartate transaminase; casein; catalase; erythrocyte sedimentation rate; fingerlings; gelatin; glutathione peroxidase; hematocrit; hemoglobin; immune response; intestines; lysozyme; malondialdehyde; regression analysis; satiety; specific growth rate; superoxide dismutase; tryptophan; weight gain
Abstract:
... In this investigation, the tryptophan requirement of C. punctatus fingerling (5.45 ± 0.07 g) was determined by feeding seven isoproteic (450 g kg⁻¹ CP) and isoenergetic (14.7 kJ g⁻¹ DE) diets with graded levels of tryptophan 1, 2, 3, 4, 5, 6, and 10 g kg⁻¹, respectively to randomly stocked groups in triplicate at 8:00, 12:30 and 17:30 h near to satiety for 12 weeks. Tryptophan contributed by casei ...
DOI:
10.1016/j.aquaculture.2022.738745

11. Dual-ratiometric electrochemical aptasensor based on carbon nanohorns/anthraquinone-2-carboxylic acid/Au nanoparticles for simultaneous detection of malathion and omethoate

Author:
Chengxi Zhu; Xiaohong Liu; Yuye Li; Dongmei Yu; Qian Gao; Lixing Chen
Source:
Talanta 2023 v.253 pp. 123966
ISSN:
0039-9140
Subject:
DNA; ambient temperature; aptasensors; carbon; cross reaction; electrochemistry; electrodes; human health; malathion; nanogold; oligonucleotides; omethoate
Abstract:
... Organophosphorus pesticides (OPs) are one of the most frequently used pesticides in agriculture, and their residues in environment have caused serious human health and environmental concerns. In this work, we reported a dual-ratiometric electrochemical aptasensor based on carbon nanohorns/anthraquinone-2-carboxylic acid/Au nanoparticles (CNHs/AQ/AuNPs) for simultaneous detection of malathion (MAL) ...
DOI:
10.1016/j.talanta.2022.123966

12. Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement

Author:
Cuiying Lin; Qingqing Huang; Mengjian Tian; Fang Luo; Jian Wang; Bin Qiu; Shuofei Yang; Zhenyu Lin
Source:
Talanta 2023 v.251 pp. 123748
ISSN:
0039-9140
Subject:
DNA; adenine; biosensors; blood serum; detection limit; electrochemiluminescence; electrodes; ferrocenes; indium; methylation; methyltransferases; restriction endonucleases; tin
Abstract:
... In this work, an electrochemiluminescence (ECL) biosensor with dual signal enhancement was constructed and used for DNA adenine methylation methyltransferase (Dam MTase) detection. At present of Dam MTase, restriction endonuclease (DPnI) cleaves hairpin DNA (HP) and releases the HP stem end as a single strand that can activate CRISPR/Cas12a trans-cleavage activity. Assisted by trans-cleavage, the ...
DOI:
10.1016/j.talanta.2022.123748

13. Epinecidin-1, a marine antifungal peptide, inhibits Botrytis cinerea and delays gray mold in postharvest peaches

Author:
Li Fan; Yingying Wei; Yi Chen; Shu Jiang; Feng Xu; Chundan Zhang; Hongfei Wang; Xingfeng Shao
Source:
Food chemistry 2023 v.403 pp. 134419
ISSN:
0308-8146
Subject:
Botrytis cinerea; DNA; antifungal properties; antimicrobial peptides; cell structures; food chemistry; gray mold; mycelium; peaches; permeability
Abstract:
... This study investigatedthe mechanism of epinecidin-1 against Botrytis cinerea, in vitro, and its effectiveness at inhibiting gray mold on postharvest peach fruit. We found that in vitro, epinecidin-1 had significantly greater antifungal activity against B. cinerea than either clavanin-A or mytimycin, two other marine derived antimicrobial peptides that we tested. Its antifungal activity was heat-r ...
DOI:
10.1016/j.foodchem.2022.134419

14. Gene transcripts responsive to drought stress identified in Citrus macrophylla bark tissue transcriptome have a modified response in plants infected by Citrus tristeza virus

Author:
Melina C. da Silva; Patrícia I.S. Pinto; Rui Guerra; Amílcar Duarte; Deborah M. Power; Natália T. Marques
Source:
Scientia horticulturae 2023 v.307 pp. 111526
ISSN:
0304-4238
Subject:
Citrus macrophylla; Citrus tristeza virus; abscisic acid; bark; brassinosteroids; calcium; drought; drought tolerance; gene expression regulation; genes; genetic selection; molecular weight; phenylpropanoids; phosphotransferases (kinases); photosynthesis; proteins; quantitative polymerase chain reaction; salicylic acid; sequence analysis; transcriptome; water shortages; water stress
Abstract:
... Citrus macrophylla Wester (CM) has valuable agronomic characteristics such as the ability to grow in saline soils, although with low tolerance to prolonged drought stress (DS). To understand the mechanisms that characterize CM response to water scarcity, this study compared transcriptome profile changes in CM stem tissue when exposed to DS and identified a total of 2745 differentially expressed tr ...
DOI:
10.1016/j.scienta.2022.111526

15. Genetic diversity analysis and core collection construction for Amaranthus tricolor germplasm based on genome-wide single-nucleotide polymorphisms

Author:
Ken Hoshikawa; Ya-Ping Lin; Roland Schafleitner; Kenta Shirasawa; Sachiko Isobe; Duc Chinh Nguyen; Ryo Ohsawa; Yosuke Yoshioka
Source:
Scientia horticulturae 2023 v.307 pp. 111428
ISSN:
0304-4238
Subject:
Amaranthus tricolor; DNA; gene banks; genetic variation; germplasm; nutrient content; population structure; vegetables; Asia
Abstract:
... The genus Amaranthus includes 60 to 70 species, some used as grain or leafy vegetable crops, others as ornamental crops. A. tricolor is used as a traditional leafy vegetable in Asia. It has a remarkably valuable nutrient composition and a high tolerance to environmental stresses but has been neglected in modern breeding. Detecting DNA polymorphisms among accessions helps in understanding the genet ...
DOI:
10.1016/j.scienta.2022.111428

16. Genome–wide association study and genomic prediction of growth traits in bighead catfish (Clarias macrocephalus Günther, 1864)

Author:
Prapaiphan Chaivichoo; Sila Sukhavachana; Rabuesak Khumthong; Prapansak Srisapoome; Satid Chatchaiphan; Uthairat Na-Nakorn
Source:
Aquaculture 2023 v.562 pp. 738748
ISSN:
0044-8486
Subject:
Clarias macrocephalus; DNA; aquaculture; body weight; catfish; cost effectiveness; genes; genetic improvement; genetic variance; genome-wide association study; genomics; genotyping; heritability; marker-assisted selection; parentage; phenotype; prediction; technology; variance; zinc finger motif
Abstract:
... Next-generation sequencing (NGS) enables cost-effective exploration of genome-wide single nucleotide polymorphisms (SNPs). This new technology has made enormous contributions in various fields including genetic improvement. This study was conducted to identify SNPs that associated with growth traits (body weight, standard length, and total length) of bighead catfish (Clarias macrocephalus Günther, ...
DOI:
10.1016/j.aquaculture.2022.738748

17. High-performance strategy for the construction of electrochemical biosensor for simultaneous detection of miRNA-141 and miRNA-21 as lung cancer biomarkers

Author:
Ali Khodadoust; Navid Nasirizadeh; Seyed Morteza Seyfati; Ramezan Ali Taheri; Mostafa Ghanei; Hasan Bagheri
Source:
Talanta 2023 v.252 pp. 123863
ISSN:
0039-9140
Subject:
DNA; DNA probes; biomarkers; biosensors; glassy carbon electrode; hybridization; lung neoplasms; microRNA; signal-to-noise ratio
Abstract:
... In this study, the dual signal-labeled hairpin-structured DNA (dhDNA)-based probes have been developed to construct a novel nano-biosensor. This one hairpin-structured probe consists of a thiolated methylene blue-labeled hairpin capture probe (MB-HCP) as an inner reference probe and a ferrocene-modified anti-miRNA-21 DNA probe (Fc-AP-21). This novel integrated structure of MB-HCP and Fc-AP-21 was ...
DOI:
10.1016/j.talanta.2022.123863

18. Improved sperm DNA integrity and altered fat metabolism and intestinal morphology in Nile tilapia (Oreochromis niloticus) males fed rations with purified nucleotide

Author:
Robie Allan Bombardelli; Juliano Kelvin dos Santos Henriques; Marta Margarete Cestari; Antônio Ernesto Meister Luz Marques; Taís Voelkl Chagas; Fabio Meurer
Source:
Aquaculture 2023 v.562 pp. 738682
ISSN:
0044-8486
Subject:
DNA; Oreochromis niloticus; aquaculture; blood glucose; chromatin; diet; digestible energy; digestible protein; enterocytes; fish; gonadosomatic index; hepatosomatic index; high density lipoprotein; lipid content; lipid metabolism; liver; morphometry; muscles; nucleotides; testes; triacylglycerols; visceral fat; viscosity
Abstract:
... In this study, we assessed the health, growth, sperm characteristics, and intestinal, liver, and testicular morphology of Nile tilapia (Oreochromis niloticus) males fed rations containing purified nucleotides (NT). A total of 420 females (286 ± 7.15 g) and 140 males (536 ± 13.4 g) were used. Fish were fed on extruded ration (280 g digestible protein kg⁻¹; 11.72 M joule (MJ) of digestible energy kg ...
DOI:
10.1016/j.aquaculture.2022.738682

19. Insights into a novel nitrogen removal process based on simultaneous anammox and denitrification (SAD) following nitritation with in-situ NOB elimination

Author:
Jing Chen; Jia Zeng; Yiran He; Shiquan Sun; Haipeng Wu; Yaoyu Zhou; Zhenguo Chen; Jianhui Wang; Hong Chen
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 160-170
ISSN:
1001-0742
Subject:
Comamonadaceae; DNA; anaerobic ammonium oxidation; batch systems; denitrification; nitrogen; total nitrogen; wastewater; wastewater treatment; zeolites; China
Abstract:
... Simultaneous anammox and denitrification (SAD) is an efficient approach to treat wastewater having a low C/N ratio; however, few studies have investigated a combination of SAD and partial nitritation (PN). In this study, a lab-scale up-flow blanket filter (UBF) and zeolite sequence batch reactor (ZSBR) were continuously operated to implement SAD and PN advantages, respectively. The UBF achieved a ...
DOI:
10.1016/j.jes.2022.01.019

20. Integrated multi-omic analyses on yellow Flammulina filiformis cultivar reveal postharvest oxidative damage responses

Author:
Yu Fu; Hao Tan; Bo Wang; Weihong Peng; Qun Sun; Yang Yu
Source:
Postharvest biology and technology 2023 v.195 pp. 112111
ISSN:
0925-5214
Subject:
DNA; Flammulina; biosynthesis; catechol oxidase; cell senescence; cultivars; genome; laccase; melanin; mushrooms; oxidative phosphorylation; oxygen; proteolysis; proteome; proteomics; technology; transcriptome; transcriptomics; tyrosine
Abstract:
... Yellow cultivars of Flammulina filiformis are prone to browning, which causes significant yearly loss. This study investigated the critical factors involved and revealed that oxygen plays an essential role in the browning process. Integrated multi-omic analyses, including genome sequencing, transcriptome profiling, and 4D label-free proteome quantification, were incorporated to dissect essential g ...
DOI:
10.1016/j.postharvbio.2022.112111

21. Interaction of Polycyclic Aromatic Hydrocarbon compounds in fish primary hepatocytes: From molecular mechanisms to genotoxic effects

Author:
Isabella Bramatti; Beatriz Matos; Neusa Figueiredo; Pedro Pousão-Ferreira; Vasco Branco; Marta Martins
Source:
Science of the total environment 2023 v.855 pp. 158783
ISSN:
0048-9697
Subject:
DNA; DNA damage; Sparus aurata; Western blotting; carcinogenicity; cell viability; chemical structure; comet assay; environment; environmental assessment; fish; genes; genotoxicity; hepatocytes; metabolites; mutagens; phenanthrenes; protein synthesis; risk assessment
Abstract:
... Polycyclic Aromatic Hydrocarbons (PAHs) are persistent pollutants normally found in the environment as complex mixtures. Although several individual PAHs are classified as mutagenic and carcinogenic pollutants, the interaction effects between compounds in a mixture may trigger different toxicological mechanisms and, consequently, yield different effects to organisms which are not accounted for in ...
DOI:
10.1016/j.scitotenv.2022.158783

22. Investigation of the latency of Cyprinid herpesvirus 2 in apparently healthy farmed gibel carp, Carassius auratus gibelio

Author:
Chang Wei; Chaonan Xu; Yuyu Sun; Jinzhou Li; Motohiko Sano; Qiang Li
Source:
Aquaculture 2023 v.562 pp. 738854
ISSN:
0044-8486
Subject:
Carassius auratus; Carassius gibelio; Cyprinid herpesvirus 2; DNA; aquaculture; carrier state; gene expression; genes; goldfish; kidneys; necrosis; pathogens; transcription (genetics); viruses
Abstract:
... Cyprinid herpesvirus 2 (CyHV-2) is a highly contagious pathogen that causes herpesviral haematopoietic necrosis (HVHN) disease to goldfish, Carassius auratus and gibel carp, C. auratus gibelio. In this study, the virus persistence or latency in apparently healthy farmed gibel carp and reactivation in vitro were investigated. All of the freshly dissected kidney tissues of 42 individuals of asymptom ...
DOI:
10.1016/j.aquaculture.2022.738854

23. Isothermal amplification based on specific signal extraction and output for fluorescence and colorimetric detection of nucleic acids

Author:
Shuya He; Yan Zhou; Yaoqi Xie; Kaijiong Zhang; Qiao He; Gang Yin; Haimin Zou; Qiongying Hu; Shilu Zhang; Hongfei He; Dongsheng Wang
Source:
Talanta 2023 v.252 pp. 123823
ISSN:
0039-9140
Subject:
DNA; DNA probes; Hepatitis B virus; colorimetry; fluorescence; loop-mediated isothermal amplification; point-of-care systems
Abstract:
... Due to the complexity of compositions and low abundance of target in clinical sample, nucleic acids detection often suffers from false positives caused by nonspecific amplification. In in vitro diagnosis (IVD), PCR usually employ TaqMan probe to report specific signals and block false positive signals. However, nucleic acid isothermal amplifications, such as loop-mediated isothermal amplification ...
DOI:
10.1016/j.talanta.2022.123823

24. It's what's inside that counts: DNA-barcoding of porcini (Boletus sp., Basidiomycota) commercial products reveals product mislabelling

Author:
Mauro Rivas-Ferreiro; Alberto Otero; Paloma Morán
Source:
Food control 2023 v.144 pp. 109346
ISSN:
0956-7135
Subject:
Boletus edulis; DNA; DNA barcoding; Hypomyces; Imleria badia; food safety; fraud; humans; internal transcribed spacers; laws and regulations; markets; mushrooms; pathogens; phylogeny; prices
Abstract:
... In the edible mushroom market, porcini (Boletus edulis Bull., and related species) are a very economically valuable product, with 20–100 tons of these mushrooms being sold each year worldwide. However, products sold as “Boletus edulis” are often a mix of different species, some of them of less value than “Boletus edulis” but sold at the same price. DNA-Barcoding is a powerful tool that can help un ...
DOI:
10.1016/j.foodcont.2022.109346

25. Label free flexible electrochemical DNA biosensor for selective detection of Shigella flexneri in real food samples

Author:
M.R. Ali; M.S. Bacchu; S. Das; S. Akter; M.M. Rahman; M.Aly Saad Aly; M.Z.H. Khan
Source:
Talanta 2023 v.253 pp. 123909
ISSN:
0039-9140
Subject:
DNA; Shigella flexneri; biosensors; detection limit; electrochemistry; electrodes; foodborne bacterial pathogens; indium tin oxide; melamine; sodium
Abstract:
... An effective tool for early-stage selective detection of the foodborne bacterial pathogen Shigella flexneri (S. flexneri) is essential for diagnosing infectious diseases and controlling outbreaks. Here, a label-free electrochemical DNA biosensor for monitoring S. flexneri is developed. To fabricate the biosensor, detection probe (capture probe) is immobilized on the surface of poly melamine (P-Mel ...
DOI:
10.1016/j.talanta.2022.123909

26. Label-free detection of DNA methylation by surface-enhanced Raman spectroscopy using zirconium-modified silver nanoparticles

Author:
Yuan Zhang; De-Sheng Zhan; Xiao-Ying Xu; Zhe Zhang; Mahmoud Elsayed Hafez; Yue He; Yang Li; Da-Wei Li
Source:
Talanta 2023 v.253 pp. 123941
ISSN:
0039-9140
Subject:
DNA; DNA fingerprinting; DNA methylation; Raman spectroscopy; adenine; cytosine; epigenetics; genes; hydrophobicity; nanosilver; principal component analysis; zirconium
Abstract:
... DNA methylation is an important feature of gene epigenetics that affects the metabolic process of organisms. Although surface-enhanced Raman spectroscopy (SERS) has demonstrated great potential in label-free DNA detection, discriminating the various processes involved in DNA methylation remains a challenge. DNA molecules fold themselves, wrapping the hydrophobic bases, thus making it difficult for ...
DOI:
10.1016/j.talanta.2022.123941

27. Methylome and transcriptome analysis of alters leaf phenotype with autotetraploid in grape

Author:
Xiaodong Xiang; Yukun Gao; Jianghui Cui; Genzeng Ren; Congpei Yin; Jinhua Chang
Source:
Scientia horticulturae 2023 v.307 pp. 111534
ISSN:
0304-4238
Subject:
5-methylcytosine; DNA; Vitis vinifera; anthocyanins; antioxidant activity; autotetraploidy; biosynthesis; bisulfites; cell walls; diploidy; epigenetics; gene expression; gene expression regulation; genomics; grapes; homeostasis; introns; leaf thickness; leaves; lipid metabolism; methylation; phenotype; photosynthesis; plant hormones; secondary metabolites; transcriptomics
Abstract:
... The doubling of the plant genome causes obvious morphological and physiological changes, and DNA 5-methylcytosine (5mCs) modification is a key epigenetic component that helps neopolyploids overcome challenges and acquire advantages. In the study of autotetraploid grape, we found that leaf thickness, photosynthetic and antioxidant capacity increased and anthocyanin coloring decreased in autotetrapl ...
DOI:
10.1016/j.scienta.2022.111534

28. Mitochondrial cytochrome oxidase 1 reveals genetic diversity of the African Snakehead fish Parachanna obscura, Gunther, 1861 from Nigeria's freshwater environment

Author:
Friday Elijah Osho; Bamidele Oluwarotimi Omitoyin; Emmanuel Kolawole Ajani; Victor O. Azuh; Adedapo Olutola Adediji
Source:
Aquaculture and fisheries 2023 v.8 no.1 pp. 59-66
ISSN:
2468-550X
Subject:
DNA; Parachanna; aquaculture; cytochrome-c oxidase; freshwater; genes; genetic variation; haplotypes; mitochondria; phylogeny; polymerase chain reaction; rivers; topology; variance; Nigeria
Abstract:
... The study investigated the genetic variation of Parachanna obscura from five rivers (Anambra, Ibbi, Imo, Katsina-Ala and Ogun) in Nigeria using the mitochondrial cytochrome oxidase 1 gene. DNA was extracted from 19, 22, 16, 18 and 21 fin clips per river population, respectively and subjected to polymerase chain reaction. A total of 96 sequences, each with 671 bp were obtained with 38 (5.6%) polymo ...
DOI:
10.1016/j.aaf.2021.08.007

29. Nano-gold mediated aptasensor for colorimetric monitoring of acrylamide: Smartphone readout strategy for on-site food control

Author:
Zahra Khoshbin; Marzieh Moeenfard; Khalil Abnous; Seyed Mohammad Taghdisi
Source:
Food chemistry 2023 v.399 pp. 133983
ISSN:
0308-8146
Subject:
DNA; acrylamides; aptasensors; breads; color; colorimetry; food chemistry; food safety; mobile telephones; nanogold
Abstract:
... A simple aptasensor is embedded in the internal surface of a micropipette tip as the aptasensor substrate for the label-free monitoring of acrylamide. The aptasensor is based on the formation of the triple-helix molecular switch (THMS) structure of the DNA strands that induces the salt-enforced aggregation of gold nanoparticles (AuNPs). A smartphone imaging readout-based strategy is applied to qua ...
DOI:
10.1016/j.foodchem.2022.133983

30. Nondestructive and rapid method for sex identification of the tropical sea cucumber Holothuria scabra by anal swab sampling

Author:
Zixuan E; Chuhang Cheng; Feifei Wu; Chunhua Ren; Rong Chen; Yingzhu Rao; Bo Ma; Xiao Jiang; Peng Luo; Xiaomin Li; Xin Zhang; Fajun Jiang; Chaoqun Hu; Ting Chen
Source:
Aquaculture 2023 v.562 pp. 738749
ISSN:
0044-8486
Subject:
DNA; Holothuria scabra; aquaculture; dimorphism; females; genomics; loop-mediated isothermal amplification; males; phenotype; rapid methods; reproduction; South East Asia
Abstract:
... As one of the most economically significant sea cucumber species, Holothuria scabra are naturally distributed in the Indo-West Pacific region and are widely cultural in Southeast Asia. The appearances of female and male H. scabra have no obvious phenotypic dimorphism. Thus, identification of sex-specific markers and establishment of sex detection methods are beneficial to artificially induced repr ...
DOI:
10.1016/j.aquaculture.2022.738749

31. Paper functionalization for detection of Plasmodium falciparum DNA using square waves voltammetry

Author:
Romain Clément; Anne-lise Bienvenu; Adeline Lavoignat; Guillaume Bonnot; Bastien Doumèche; Stéphane Picot
Source:
Talanta 2023 v.252 pp. 123839
ISSN:
0039-9140
Subject:
DNA; Plasmodium falciparum; loop-mediated isothermal amplification; malaria; methylene blue; mobile telephones; paper; parasitemia; point-of-care systems; voltammetry
Abstract:
... Malaria elimination is a major goal to be reached in the next decade. Significant progress were made in the past, and the prevalence decreased in many areas while the positive trend stalled in the last years. The exact number of asymptomatic carriers of Plasmodium parasites is unknown since this population is not detected by conventional diagnosis methods and participate in the maintenance of tran ...
DOI:
10.1016/j.talanta.2022.123839

32. Phenolics from noni (Morinda citrifolia L.) fruit alleviate obesity in high fat diet-fed mice via modulating the gut microbiota and mitigating intestinal damage

Author:
Ruimin Wang; Lu Wang; Shengwei Wang; Juntao Wang; Congyan Su; Lin Zhang; Congfa Li; Sixin Liu
Source:
Food chemistry 2023 v.402 pp. 134232
ISSN:
0308-8146
Subject:
Morinda citrifolia; anti-obesity effects; dysbiosis; food chemistry; fruits; high fat diet; inflammation; intestinal microorganisms; intestines; lipopolysaccharides; liver; obesity; occludins; oxidative stress; phenolic compounds; protein synthesis; short chain fatty acids
Abstract:
... Noni fruit has certain anti-obesity effect. However, the bioactive ingredients in noni fruit that contribute to anti-obesity activity as well as the relation between its anti-obesity activity and gut microbiota remain unclear. In this study, obese mice induced by high-fat diet (HFD) and were intervened with noni fruit phenolic extract (NFE) for 10 weeks. The results showed NFE supplementation decr ...
DOI:
10.1016/j.foodchem.2022.134232

33. Phylogeny, character evolution, and biogeography of the fern genus Bolbitis (Dryopteridaceae)

Author:
Li-Yun Nie; Liang Zhang; Zhen-Long Liang; Rossarin Pollawatn; Yue-Hong Yan; Ngan Thi Lu; Ralf Knapp; Xia Wan; Daniele Cicuzza; Xin-Xin Chen; Hong-Feng Chen; Ai-Hua Wang; Yu-Jie Liao; Fa-Guo Wang; Li-Bing Zhang
Source:
Molecular phylogenetics and evolution 2023 v.178 pp. 107633
ISSN:
1055-7903
Subject:
Bolbitis; DNA; biogeography; ferns and fern allies; geographical distribution; monophyly; paraphyly; polyphyly; Africa; Asia
Abstract:
... Bolbitis is a pantropical fern genus of Dryopteridaceae with ca. 80 species mainly in tropical Asia. Earlier studies confirmed the monophyly of Bolbitis when Mickelia is excluded and identified three major clades in Bolbitis. However, earlier studies are based on relatively small sampling and the majority of Asian species are not sampled. In this study, DNA sequences of three plastid markers of 16 ...
DOI:
10.1016/j.ympev.2022.107633

34. Population structure of Caspian Kutum (Rutilus frisii, Nordmann, 1840) in the southern coast of Caspian Sea using Genome-wide single nucleotide polymorphism markers

Author:
Mahdieh Rahbar; Majidreza Khoshkholgh; Sajad Nazari
Source:
Fisheries research 2023 v.257 pp. 106499
ISSN:
0165-7836
Subject:
Bayesian theory; DNA; Rutilus frisii; Rutilus kutum; coasts; endangered species; fish; gene frequency; genetic variation; genomics; heterozygosity; indigenous species; loci; multidimensional scaling; population structure; research; single nucleotide polymorphism; Caspian Sea; Iran
Abstract:
... The Caspian kutum (Rutilus frisii, Nordmann, 1840) is one of the most important semi-anadromous endemic fish species in the southern part of the Caspian Sea. The fisheries from wild populations of R. frisii are declined drastically due to variability of sea levels, overfishing, and illegal fishing. For this regard, genetic studies is very noticed to maintain of remained this endangered species. He ...
DOI:
10.1016/j.fishres.2022.106499

35. Proteomics revealed the effects of nucleic acid metabolism, transcription and translation on postharvest broccoli senescence under elevated O2 stress

Author:
Yuxiao Zhang; Ying Chen; Yanyin Guo; Yangli Ma; Mei Yang; Ruiqing Fu; Yupeng Sun; Jing Qin
Source:
Postharvest biology and technology 2023 v.195 pp. 112110
ISSN:
0925-5214
Subject:
DNA; DNA replication; RNA; biochemical pathways; broccoli; carbon dioxide; fruits; gene expression regulation; proteasome endopeptidase complex; protein synthesis; proteomics; technology
Abstract:
... Nucleic acids (DNA and RNA) play crucial roles in guiding protein synthesis and plant resistance to biotic and abiotic stresses, but our current available understanding concerning on the role and function of nucleic acid metabolism, transcription and translation processes in postharvest fruit and vegetables remains largely unclear. In this study, an iTRAQ-based proteomics was used to investigate c ...
DOI:
10.1016/j.postharvbio.2022.112110

36. Real-time PCR, and Recombinase Polymerase Amplification combined with SYBR Green I for naked-eye detection, along with Propidium Monoazide (PMA) for the detection of viable patulin-producing fungi in apples and by-products

Author:
Foteini Roumani; Jorge Barros-Velázquez; Alejandro Garrido-Maestu; Marta Prado
Source:
Food control 2023 v.144 pp. 109347
ISSN:
0956-7135
Subject:
DNA; Penicillium; biochemical pathways; food safety; genes; patulin; propidium; quantitative polymerase chain reaction; recombinase polymerase amplification; toxicity
Abstract:
... Nowadays, the application of rapid molecular-based methods such as PCR/qPCR and isothermal techniques like Recombinase Polymerase Amplification (RPA), has increased in order to overcome some of the drawbacks of traditional culture-based methods due to their high sensitivity and specificity. One of the main limitations of these techniques is their inability to differentiate between live and dead mi ...
DOI:
10.1016/j.foodcont.2022.109347

37. Role of 19 SNPs in 10 genes with type 2 diabetes in the Pakistani population

Author:
Netasha Khan; Andrew D. Paterson; Delnaz Roshandel; Saadia Maqbool; Nosheen Fazal; Liaqat Ali; Raja Khurram; Sundus Ijaz Maqsood; Syeda Hafiza Benish Ali; Hira Khan; Khadija Tariq; Saira Bano; Aisha Azam; Irfan Muslim; Maleeha Maria; Zehra Agha; Mujtaba Babar; Nadia Khalida Waheed; Maleeha Azam; Raheel Qamar
Source:
Gene 2023 v.848 pp. 146899
ISSN:
0378-1119
Subject:
DNA; confidence interval; diabetic retinopathy; genes; genotyping; noninsulin-dependent diabetes mellitus; odds ratio; regression analysis; risk
Abstract:
... Meta-GWAS report numerous variants associated with type 2 diabetes (T2D), however, for diabetic retinopathy (DR) no loci achieved genome-wide significance. There are limited candidate gene analyses for T2D and/or DR reported from the Pakistani population. Therefore, the current study was designed to evaluate the genetic association of 10 loci with T2D, non-proliferative DR (NPDR), and proliferativ ...
DOI:
10.1016/j.gene.2022.146899

38. Synergistic disinfection of aerosolized bacteria and bacteriophage by far-UVC (222-nm) and negative air ions

Author:
Y.H. Lu; H. Wu; H.H. Zhang; W.S. Li; A.C.K. Lai
Source:
Journal of hazardous materials 2023 v.441 pp. 129876
ISSN:
0304-3894
Subject:
DNA; Escherichia coli; Staphylococcus epidermidis; air; bacteriophages; bioaerosols; disinfection; ultraviolet radiation
Abstract:
... Air ionizers and 222-nm krypton-chlorine (KrCl) excilamp have proven to be effective disinfection apparatus for bacteria and viruses with limited health risks. We determined inactivation efficiencies by operating them individually and in combined modules. Gram-positive and gram-negative bacteria, non-enveloped dsDNA virus, and enveloped dsRNA virus were examined in a designed air disinfection syst ...
DOI:
10.1016/j.jhazmat.2022.129876

39. TMT-based quantitative proteomic and physiological analyses on lotus plumule of artificially aged seed in long-living sacred lotus Nelumbo nucifera

Author:
Dongli He; Mengmeng Cai; Meihui Liu; Pingfang Yang
Source:
Journal of proteomics 2023 v.270 pp. 104736
ISSN:
1874-3919
Subject:
DNA; Nelumbo nucifera; RNA; cell membranes; germination; histology; longevity; nutritive value; photosynthesis; physiological state; plumule; protein composition; proteome; proteomics; seed longevity; seed quality; starch; sucrose; vigor
Abstract:
... Seed longevity is important for the maintenance of seed nutritional quality, vigor, and germination potential during storage. Sacred lotus is known as one of the longest living seeds in the world and their ability to maintain longevity has been widely investigated. In this study, a suitable controlled deterioration treatment (CDT) method was first established to evaluate the vigor loss of lotus pl ...
DOI:
10.1016/j.jprot.2022.104736

40. Temporal profiling resolves the drivers of microbial nitrogen cycling variability in coastal sediments

Author:
Alexis J. Marshall; Lori Phillips; Andrew Longmore; Helen L. Hayden; Karla B. Heidelberg; Caixian Tang; Pauline Mele
Source:
Science of the total environment 2023 v.856 pp. 159057
ISSN:
0048-9697
Subject:
Archaea; DNA; RNA; ammonium; anaerobic ammonium oxidation; coastal zone management; detection limit; environment; genes; microbial activity; microbial nitrogen; nitrates; nitrites; nitrogen fixation; oxidation; phylogeny; seasonal variation; sediments; Australia
Abstract:
... Here we describe the potential for sediment microbial nitrogen-cycling gene (DNA) and activity (RNA) abundances to spatially resolve coastal areas impacted by seasonal variability in external nutrient inputs. Three sites were chosen within a nitrogen-limited embayment, Port Phillip Bay (PPB), Australia that reflect variability in both proximity to external nutrient inputs and the dominant form of ...
DOI:
10.1016/j.scitotenv.2022.159057

41. Understanding the influence of the bioaerosol source on the distribution of airborne bacteria in hospital indoor air

Author:
Víctor Pertegal; Engracia Lacasa; Pablo Cañizares; Manuel A. Rodrigo; Cristina Sáez
Source:
Environmental research 2023 v.216 pp. 114458
ISSN:
0013-9351
Subject:
DNA; Escherichia coli; World Health Organization; air; air flow; bioaerosols; cell membranes; hospitals; models; osmotic pressure; relative humidity; research; saliva; urine; wastewater
Abstract:
... The composition and concentration of airborne microorganisms in hospital indoor air has been reported to contain airborne bacteria and fungi concentrations ranged 10¹–10³ CFU/m³ in inpatients facilities which mostly exceed recommendations from the World Health Organization (WHO). In this work, a deeper knowledge of the performance of airborne microorganisms would allow improving the designs of the ...
DOI:
10.1016/j.envres.2022.114458

42. Unraveling the mechanism of the synergistic antimicrobial effect of cineole and carvacrol on Escherichia coli O157:H7 inhibition and its application on fresh-cut cucumbers

Author:
Keren Agyekumwaa Addo; Hui Li; Yigang Yu; Xinglong Xiao
Source:
Food control 2023 v.144 pp. 109339
ISSN:
0956-7135
Subject:
DNA; Escherichia coli O157; Shiga toxin; antimicrobial properties; bacteria; biofilm; carvacrol; cineole; exopolysaccharides; flagellum; food safety; fresh produce; genes; intimin; mechanism of action; quorum sensing; sanitizers; transcription (genetics); virulence
Abstract:
... The objective of this study was to evaluate the mechanism of the synergistic antimicrobial effect of cineole (CN) and carvacrol (CR) on E. coli and its application on fresh-cut cucumbers. Herein, the CN/CR synergy could effectively inhibit E. coli by destroying its bacterial cell structure and causing the leakage of intracellular organic matters. For the first time, CN/CR synergy interaction on E. ...
DOI:
10.1016/j.foodcont.2022.109339

43. Virus-induced gene silencing (VIGS) for functional analysis of genes involved in the regulation of anthocyanin biosynthesis in the perianth of Phalaenopsis-type Dendrobium hybrids

Author:
Tianze Hou; Mingzhong Huang; Yi Liao; Shunjiao Lu; Zongxing Long; Junmei Yin; Chonghui Li
Source:
Scientia horticulturae 2023 v.307 pp. 111485
ISSN:
0304-4238
Subject:
Cymbidium mosaic virus; DNA; Dendrobium; agroinfiltration; anthocyanins; biochemical pathways; biosynthesis; color; genetic markers; genetic transformation; perianth; phenotype; pigmentation; regulator genes
Abstract:
... Virus-induced gene silencing (VIGS) is an efficient way to study floral gene functions of orchids that have long life cycle and inefficient genetic transformation. However, reports of the use of the VIGS technique for Dendrobium spp. are scarce. To explore the applicability of Cymbidium mosaic virus (CymMV)-based VIGS in Phalaenopsis-type Dendrobium hybrids (Den-Phals), the regulator gene DhMYB2 o ...
DOI:
10.1016/j.scienta.2022.111485

44. Wash-free photoelectrochemical DNA detection based on photoredox catalysis combined with electroreduction and light blocking by magnetic microparticles

Author:
Jihyeon Kim; Seonhwa Park; Haesik Yang
Source:
Talanta 2023 v.253 pp. 123872
ISSN:
0039-9140
Subject:
DNA; biosensors; blood serum; catalysts; catalytic activity; electrochemistry; electrodes; electron transfer; energy transfer; eosin; indium tin oxide; light intensity; magnetism; photoirradiation; photosensitivity
Abstract:
... To obtain a sensitive, wash-free photoelectrochemical biosensor based on electron mediation between an electrode and a photoredox catalyst (PC) label, unavoidable O₂-related reactions should have no effect or be beneficial, and the rate of electron mediation should depend on the distance between the PC label and electrode. A wash-free photoelectrochemical biosensor that (i) combines photoredox cat ...
DOI:
10.1016/j.talanta.2022.123872

45. XNA probe and CRISPR/Cas12a-powered flexible fluorescent and electrochemical dual-mode biosensor for sensitive detection of m6A site-specific RNA modification

Author:
Qinli Pu; Yuanyuan Ye; Juan Hu; Cong Xie; Xi Zhou; Hongyan Yu; Fangli Liao; Song Jiang; Linshan Jiang; Guoming Xie; Weixian Chen
Source:
Talanta 2023 v.252 pp. 123754
ISSN:
0039-9140
Subject:
DNA; RNA; biosensors; electrochemistry; fluorescence; hybridization; methylation; reverse transcriptase polymerase chain reaction; thermodynamics
Abstract:
... N6-methyladenosine (m6A) in RNAs is closely related to various biological progresses, but the specific regulatory mechanisms are still unclear. The existing m6A single-base resolution analysis techniques have problems of specificity and sensitivity to be improved, which can hardly meet the urgent needs of basic research and clinical applications. This work proposes a new strategy based on xeno nuc ...
DOI:
10.1016/j.talanta.2022.123754

46. An autonomous driven DNA walker-based electrochemical aptasensor for on-site detection of Ochratoxin A

Author:
Tongfu Huang; Mei Wang; Nian Hong; Hanfeng Cui; Qiqi Fan; Guobing Wei; Longshua Qin; Jing Zhang; Hao Fan
Source:
Talanta 2023 v.252 pp. 123785
ISSN:
0039-9140
Subject:
DNA; aptasensors; coordination polymers; electrochemistry; ochratoxin A
Abstract:
... Herein, an autonomous driven DNA walker-based aptasensor is proposed for the detection of Ochratoxin A (OTA). A nanoscale metal-organic framework (MOF) is synthesized and used to load Mn²⁺, a cofactor for DNAzyme. The DNA walker and Mn²⁺@MOF are assembled on the Au surface, integrating the DNA walker and metal auxiliary ions. The proposed sensor avoids the addition of metal auxiliary ions of DNAzy ...
DOI:
10.1016/j.talanta.2022.123785

47. An autonomous synthetic DNA machine for ultrasensitive detection of Salmonella typhimurium based on bidirectional primers exchange reaction cascades

Author:
Qianru Li; Manru Zhang; Qingxin Zhang; Zhixue Zhu; Zhiqiang Guo; Jingjing Li; Wanqing Xu; Jingru Zhu; Yuying Yao; Zongqiang Li; Yu Wang; Jiadong Huang; Su Liu
Source:
Talanta 2023 v.252 pp. 123833
ISSN:
0039-9140
Subject:
DNA; Salmonella Typhimurium; biosensors; detection limit; fluorescence; pathogens; statistics
Abstract:
... Statistics show that food poisoning caused by Salmonella typhimurium (S. Typhimurium) often tops the list of bacterial food poisoning types in countries around the world. However, detecting traces of S. Typhimurium in real samples remains challenging. In recent years, primer exchange reaction (PER), a new isothermal amplification strategy, has rapidly attracted the attention of researchers in the ...
DOI:
10.1016/j.talanta.2022.123833

48. A biodegradable and cofactor self-sufficient aptazyme nanoprobe for amplified imaging of low-abundance protein in living cells

Author:
Jiayao Xu; Lifang Yao; Xiaohong Zhong; Kun Hu; Shulin Zhao; Yong Huang
Source:
Talanta 2023 v.253 pp. 123983
ISSN:
0039-9140
Subject:
DNA; biodegradability; detection limit; flow cytometry; fluorescence; fluorescent dyes; nanorods; neoplasms; oligonucleotides
Abstract:
... Despite the progress on the analysis of proteins either in vitro or in vivo, detection and imaging of low-abundance proteins in living cells still remains challenging. Herein, a novel biodegradable and cofactor self-sufficient DNAzyme nanoprobe has been deve-loped for catalytic imaging of protein in living cells with signal amplification capacity. This DNAzyme nanoprobe is constructed by assemblin ...
DOI:
10.1016/j.talanta.2022.123983

49. An electrochemical biosensor for the highly sensitive detection of Staphylococcus aureus based on SRCA-CRISPR/Cas12a

Author:
Luqi Huang; Ning Yuan; Wei Guo; Yunzhe Zhang; Wei Zhang
Source:
Talanta 2023 v.252 pp. 123821
ISSN:
0039-9140
Subject:
DNA; Staphylococcus aureus; biosensors; electrodes; gold
Abstract:
... As one of the major foodborne pathogens, Staphylococcus aureus (S. aureus) can cause infectious diseases. In the current study, a novel electrochemical biosensor based on saltatory rolling circle amplification (SRCA) combined with CRISPR/Cas12a system was developed for the accurate detection of S. aureus. The thio-modified reporter probes (SH-ssDNA-MB) was immobilized on the surface of gold nanopa ...
DOI:
10.1016/j.talanta.2022.123821

50. A genetic linkage map of the Pacific white shrimp (Litopenaeus vannamei): QTL mapping for low-temperature tolerance and growth-related traits and identification of the candidate genes

Author:
Huijie Lu; Wei Chen; Fengkun Liu; Minwei Huang; Kai Peng; Jichen Zhao; Xiaoying Chen; Yuping Sun; Chaozheng Li; Yihong Chen; Zhenxing Liu; Huo Li; Wen Huang
Source:
Aquaculture 2023 v.562 pp. 738834
ISSN:
0044-8486
Subject:
DNA; Litopenaeus vannamei; aquaculture; body weight; chromosome mapping; cold stress; genes; genomics; loci; marker-assisted selection; methyltransferases; missense mutation; quantitative traits; shrimp; water temperature
Abstract:
... Low water temperature is a prevailing abiotic stress which adversely impacts global shrimp growth and production. In this study, a high-density genetic linkage map of L. vannamei was constructed using double-digest restriction site-associated DNA sequencing (ddRAD-seq) with an F1 full-sib family mapping panel comprising 148 progenies and 2 parents. A total of 2528 single-nucleotide polymorphisms ( ...
DOI:
10.1016/j.aquaculture.2022.738834

51. A novel label-free universal biosensing platform based on CRISPR/Cas12a for biomarker detection

Author:
Xiaomei Mu; Xin Wang; Yuxin Qin; Yong Huang; Jianniao Tian; Shulin Zhao
Source:
Talanta 2023 v.251 pp. 123795
ISSN:
0039-9140
Subject:
DNA; biomarkers; biosensors; disease surveillance; enzyme activity; fluorescence; hydrolysis; microRNA; nanosilver
Abstract:
... The development of a biosensing platform with high sensitivity, high specificity, and low cost for the detection of biomarkers, especially one that is programmable and universal, is critical for disease surveillance and diagnosis, yet it remains a difficulty. Herein, we combined the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system with a fluorescent label-free biosenso ...
DOI:
10.1016/j.talanta.2022.123795

52. A novel method for detection of ochratoxin A in foods—Co-MOFs based dual signal ratiometric electrochemical aptamer sensor coupled with DNA walker

Author:
Yan Guan; Peng-Bin Si; Tong Yang; Yuan Wu; Yun-Hui Yang; Rong Hu
Source:
Food chemistry 2023 v.403 pp. 134316
ISSN:
0308-8146
Subject:
DNA; cobalt; detection limit; electrochemistry; electrodes; enzyme-linked immunosorbent assay; food chemistry; human health; kidneys; ochratoxin A; oligonucleotides; red wines; toluidine blue
Abstract:
... Ochratoxin A (OTA) is a naturally occurring mycotoxin that poses serious threats, such as kidney damage, to human health. Therefore, we developed a DNA walker-based dual-signal electrochemical ratiometric platform for OTA detection, which could overlook the variations in environmental and instrumental factors and DNA load densities. Cobalt metal–organic frameworks (Co-MOFs) and toluidine blue were ...
DOI:
10.1016/j.foodchem.2022.134316

53. The regulatory roles of DDIT4 in TDCIPP-induced autophagy and apoptosis in PC12 cells

Author:
Li Li; Lingyi Xi; Jin Wu; Zunquan Zhao; Youliang Chen; Weili Liu; Zhihui Pan; Mingzhu Liu; Danfeng Yang; Zhaoli Chen; Yanjun Fang
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 823-830
ISSN:
1001-0742
Subject:
DNA; Western blotting; apoptosis; autophagy; flame retardants; flow cytometry; humans; neurons; neurotoxicity; phosphates; phosphorylation; protective effect; rats; China
Abstract:
... Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is a commonly used organophosphate-based flame retardant and can bio-accumulate in human tissues and organs. As its structure is similar to that of neurotoxic organophosphate pesticides, the neurotoxicity of TDCIPP has raised widespread concerns. TDCIPP can increase neuronal apoptosis and induce autophagy. However, its regulatory mechanism remains un ...
DOI:
10.1016/j.jes.2022.02.046

54. The strategy of cell extract based metal organic frameworks (CE-MOF) for improved enzyme characteristics

Author:
Shumin Xu; Xianghui Qi; Song Gao; Yifeng Zhang; Hongling Wang; Yaokun Liang; Fanshu Kong; Rui Wang; Yiting Wang; Shuai Yang; Yingfeng An
Source:
Enzyme and microbial technology 2023 v.162 pp. 110134
ISSN:
0141-0229
Subject:
DNA; RNA; biocatalysis; fertilizer industry; fuel production; technology; temperature; xylan; xylanases; xylose
Abstract:
... A convenient cell extract based metal organic frameworks (CE-MOF) strategy was used to produce self-assembled hybrid microparticles of enzymes with improved characteristics. It was shown that many metal ions and enzymes could be used to construct catalytically active CE-MOF microparticles. As a proof-of-principle study, the β-xylosidase BH3683 was used to prepare FeSO₄-CE-MOF-BH3683 microparticles ...
DOI:
10.1016/j.enzmictec.2022.110134

55. 100 Years of Chromosome Research in Rye, Secale L.

Author:
Rolf Schlegel
Source:
Plants 2022 v.11 no.13 pp. -
ISSN:
2223-7747
Subject:
DNA; barley; cytogenetic analysis; fish; fluorescence; genes; grasses; haploidy; meiosis; microscopy; mitosis; reciprocal translocation; rice; rye; sequence analysis; tetraploidy; trisomics; wheat
Abstract:
... Although microscopy and genetics were still in their infancy, there are cytological results produced a hundred years ago that are still relevant today. Since the 1920s, rye has been a subject of chromosome research. It started by plotting its mitotic and meiotic chromosomes to determine genome size. After controversial evidence, it became clear that the base number is n = 7. However, structural di ...
DOI:
10.3390/plants11131753

56. 16S Amplicon Sequencing of Nitrifying Bacteria and Archaea Inhabiting Maize Rhizosphere and the Influencing Environmental Factors

Author:
Oluwatobi Esther Ayiti; Ayansina Segun Ayangbenro; Olubukola Oluranti Babalola
Source:
Agriculture (Basel) 2022 v.12 no.9 pp. -
ISSN:
2077-0472
Subject:
Archaea; DNA; Nitrobacter; Nitrococcus; Nitrosococcus; Nitrosomonas; Nitrosospira; Nitrosovibrio; Nitrospirae; agriculture; ammonia; corn; nitrates; nitrogen; rhizosphere; soil; species diversity
Abstract:
... Nitrifying bacteria and archaea are ubiquitous and can transform ammonia locked up in soil or manure into nitrate, a more soluble form of nitrogen. However, nitrifying bacteria and archaea inhabiting maize rhizosphere have not been fully explored. This study evaluates the diversity and abundance of nitrifying bacteria and archaea across different growth stages of maize using 16S amplicon sequencin ...
DOI:
10.3390/agriculture12091328

57. 19th century family saga re-told by DNA recovered from postcard stamps

Author:
Cordula Haas; Christian Körner; Andrea Sulzer; Adelgunde Kratzer
Source:
Forensic science international 2022 v.330 pp. 111129
ISSN:
0379-0738
Subject:
DNA; children; forensic sciences; males; saliva
Abstract:
... Old postcards with stamps might help unravelling historical family stories and relationships. By employing ancient DNA recovered from world war I postage stamps, we disprove a family saga of an illegitimate child born in 1887. We developed a protocol to collect DNA from saliva, trapped and protected on the backside of postage stamps glued on postcards. With replicate STR analyses we were able to a ...
DOI:
10.1016/j.forsciint.2021.111129

58. 2,7-Diazapyrenes: a brief review on synthetic strategies and application opportunities

Author:
Anindita Mukherjee; Alexey A. Akulov; Sougata Santra; Mikhail V. Varaksin; Grigory A. Kim; Dmitry S. Kopchuk; Olga S. Taniya; Grigory V. Zyryanov; Oleg. N. Chupakhin
Source:
RSC advances 2022 v.12 no.15 pp. 9323-9341
ISSN:
2046-2069
Subject:
DNA; electronics; geometry; moieties
Abstract:
... 2,7-Diazapyrenes are promising azaaromatic scaffolds with a unique structural geometry and supramolecular properties. This core moiety and its derivatives with some N-methyl cations like N-methyl-2,7,-diazapyrenium, and N,N′-dimethyl-2,7-diazapyrenium attract special attention due to their challenging photophysical properties, especially in the context of interactions with DNA and some of its mono ...
DOI:
10.1039/d2ra00260d

59. 2-Oxopurine Riboside: A Dual Fluorescent Analog and Photosensitizer for RNA/DNA Research

Author:
Naishka E. Caldero-Rodríguez; Enrique M. Arpa; Diego J. Cárdenas; Lara Martínez-Fernández; Steffen Jockusch; Sourav Kanti Seth; Inés Corral; Carlos E. Crespo-Hernández
Source:
Journal of physical chemistry 2022 v.126 no.24 pp. 4483-4490
ISSN:
1520-5207
Subject:
DNA; RNA; absorption; aqueous solutions; fluorescence; nucleosides; photolysis; photosensitizing agents; quantum mechanics; singlet oxygen; solvents
Abstract:
... There is significant interest in developing suitable nucleoside analogs exhibiting high fluorescence and triplet yields to investigate the structure, dynamics, and binding properties of nucleic acids and promote selective photosensitized damage to DNA/RNA, respectively. In this study, steady-state, laser flash photolysis, time-resolved IR luminescence, and femtosecond broad-band transient absorpti ...
DOI:
10.1021/acs.jpcb.2c01113

60. 2D DNA lattices assembled from DX-coupled tiles

Author:
Wei Zhang; Chuan Jiang; Xin Guo; Mirza Muhammad Faran Ashraf Baig; Caihong Ni; Shou-Jun Xiao
Source:
Journal of colloid and interface science 2022 v.616 pp. 499-508
ISSN:
0021-9797
Subject:
DNA; atomic force microscopy; mechanics; mica; oligonucleotides
Abstract:
... Formation of coupled double crossovers (DXs) in a circular 106-mer oligonucleotide (c106nt) could generate stable tiles with the tile core span of 10 half turns. The large-span tiles with complicated curvatures and mechanics could assemble 2D lattices under different environments. Hence, 2D DNA lattice structures based on tile types and sequences, tile packing modes of corrugation and non-corrugat ...
DOI:
10.1016/j.jcis.2022.02.038

61. 2D morphometric analysis of Arabidopsis thaliana nuclei reveals characteristic profiles of different cell types and accessions

Author:
Penka Pavlova; Martijn van Zanten; Basten L. Snoek; Hans de Jong; Paul Fransz
Source:
Chromosome research 2022 v.30 no.1 pp. 5-24
ISSN:
0967-3849
Subject:
Arabidopsis thaliana; DNA; chromocenters; fluorescence microscopy; heterochromatin; morphometry; phenotype; research; transcription (genetics); transposons
Abstract:
... Functional changes of cells upon developmental switches and in response to environmental cues are often reflected in nuclear phenotypes, showing distinctive chromatin states corresponding to transcriptional changes. Such characteristic nuclear shapes have been microscopically monitored and can be quantified after differential staining of euchromatin and heterochromatin domains. Here, we examined s ...
DOI:
10.1007/s10577-021-09673-2

62. 2q35-rs13387042 variant and the risk of breast cancer: a case–control study

Author:
Abolfazl Nesaei; Zari Naderi Ghale-noie; Asma Khorshid Shamshiri; Fahimeh Afzaljavan; Mahdi Rivandi; Amir Tajbakhsh; Fatemeh Homaei Shandiz; Alireza Pasdar
Source:
Molecular biology reports 2022 v.49 no.5 pp. 3549-3557
ISSN:
0301-4851
Subject:
DNA; alleles; blood; breast neoplasms; breasts; carcinogenesis; case-control studies; demographic statistics; etiology; genetic background; genotyping; histopathology; lifestyle; molecular biology; risk; risk assessment; statistical analysis
Abstract:
... BACKGROUND: Breast Cancer is the most frequent neoplasm diagnosed among women worldwide. Genetic background and lifestyle/environment play a significant role in the disease etiology. According to Genome-wide association studies, some single-nucleotide polymorphisms such as 2q35-rs13387042–(G/A) have been introduced to be associated with breast cancer risk and features. In this study, we aimed to e ...
DOI:
10.1007/s11033-022-07195-6

63. 3, 4-Dihydroxy-l-phenylalanine Biopolymer Cellulose DNA Adhesive Card as an Enhanced Solid-Phase One-Step DNA Extraction Method from Foodborne Pathogens in Food Samples

Author:
Carole D. V. Nanfack; Jia Yang; Xiao Yuan; Jiadi Sun; Xiulan Sun; Jian Ji
Source:
Food analytical methods 2022 v.15 no.8 pp. 2069-2083
ISSN:
1936-9751
Subject:
3,4-dihydroxyphenylalanine oxidative deaminase; DNA; Fourier transform infrared spectroscopy; L-dopa; X-ray diffraction; biopolymers; cellulose; chickens; food pathogens; infrastructure; microbial detection
Abstract:
... Deoxyribonucleic acid (DNA) extraction, which is the mainstay of molecular amplification for food pathogen detection procedure, is dependent on laboratory infrastructure, cumbersome methods, and time consumption. To tackle these dependencies, we developed an enhanced mussel-inspired cellulose card for the convenient, eco-friendly, and rapid DNA extraction from bacteria present in food. This enhanc ...
DOI:
10.1007/s12161-021-02177-z

64. A 5-color flow cytometry panel to assess plasma membrane integrity, acrosomal status, membrane lipid organization and mitochondrial activity of boar and stallion spermatozoa following liquid semen storage

Author:
Monike Quirino; Ulrike Jakop; Ana Paula Gonçalves Mellagi; Fernando Pandolfo Bortolozzo; Markus Jung; Martin Schulze
Source:
Animal reproduction science 2022 v.247 pp. 107076
ISSN:
0378-4320
Subject:
DNA; animal reproduction; boars; flow cytometry; fluorescent dyes; lipids; liquids; mitochondria; plasma membrane; propidium; rhodamines; semen; sperm quality; stallions
Abstract:
... For a more practically applicable analysis of different sperm characteristics, this study aimed to develop a 5-color flow cytometry (FC) panel to concurrently analyze four sperm parameters in liquid boar and stallion semen, using also a DNA-marker for selecting sperm cell events. From each of thirty extended boar semen doses and twelve stallion semen doses, six aliquots were taken. For evaluating ...
DOI:
10.1016/j.anireprosci.2022.107076

65. A 638‐gene phylogeny supports the recognition of twice as many species in the Malagasy endemic genus Capurodendron (Sapotaceae)

Author:
Carlos G. Boluda; Camille Christe; Yamama Naciri; Laurent Gautier
Source:
Taxon 2022 v.71 no.2 pp. 360-395
ISSN:
0040-0262
Subject:
DNA; Sapotaceae; conservation status; extinction; hardwood; new species; phylogeny; risk; statistical analysis; Madagascar
Abstract:
... The Malagasy genus Capurodendron currently accommodates 26 described species and is the largest genus of the family Sapotaceae in Madagascar. These species are frequently logged because of their valued hardwood, which potentially puts them at risk of extinction. Species‐level identifications are often problematic, and this hinders both an accurate assessment of their conservation status and the de ...
DOI:
10.1002/tax.12676

66. An 82 bp tandem repeat family typical of 3′ non-coding end of Gypsy/TAT LTR retrotransposons is conserved in Coffea spp. pericentromeres

Author:
Leonardo Adabo Cintra; Thaíssa Boldieri de Souza; Letícia Maria Parteka; Lucas Mesquita Barreto; Luiz Filipe Protasio Pereira; Marcos Letaif Gaeta; Romain Guyot; André Luís Laforga Vanzela
Source:
Genome 2022 v.65 no.3 pp. 137-151
ISSN:
1480-3321
Subject:
DNA; allotetraploidy; centromeres; chromatin; genome; microsatellite repeats; non-coding RNA; retrotransposons
Abstract:
... Coffea spp. chromosomes are very small and accumulate a variety of repetitive DNA families around the centromeres. However, the proximal regions of Coffea chromosomes remain poorly understood, especially regarding the nature and organisation of the sequences. Taking advantage of the genome sequences of C. arabica (2n = 44), C. canephora, and C. eugenioides (C. arabica progenitors with 2n = 22) and ...
DOI:
10.1139/gen-2021-0045

67. 90-day nose-only inhalation toxicity study of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in Sprague-Dawley rats

Author:
Shu-Chieh Hu; Seonggi Min; Hyun-Ki Kang; Dong-Jin Yang; Mallikarjuna Basavarajappa; Sherry M. Lewis; Kelly J. Davis; Ralph E. Patton; Matthew S. Bryant; Estatira Sepehr; Raul Trbojevich; Mason G. Pearce; Michelle E. Bishop; Wei Ding; Robert H. Heflich; MacKean P. Maisha; Robert Felton; Susan Chemerynski; Steven B. Yee; Melis Coraggio; Hans Rosenfeldt; R. Philip Yeager; Paul C. Howard; Yunan Tang
Source:
Food and chemical toxicology 2022 v.160 pp. 112780
ISSN:
0278-6915
Subject:
DNA; aerosols; air; biomarkers; blood; body weight; bone marrow; breathing; carcinogenesis; carcinogenicity; cigarettes; comet assay; food consumption; genotoxicity; histopathology; humans; inhalation toxicity; lowest observed effect level; lungs; metabolites; mutagens; nitrosamines; no observed adverse effect level; nose; propylene glycol; urine
Abstract:
... 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is one of the key tobacco-specific nitrosamines that plays an important role in human lung carcinogenesis. Repeated dose inhalation toxicity data on NNK, particularly relevant to cigarette smoking, however, is surprisingly limited. Hence, there is a lack of direct information available on the carcinogenic and potential non-carcinogenic effects o ...
DOI:
10.1016/j.fct.2021.112780

68. AAQSP increases mapping resolution of stable QTLs through applying NGS-BSA in multiple genetic backgrounds

Author:
Xiaoyu Wang; Xiaowei Zhang; Daoran Fan; Juwu Gong; Shaoqi Li; Yujie Gao; Aiying Liu; Linjie Liu; Xiaoying Deng; Yuzhen Shi; Haihong Shang; Yuanming Zhang; Youlu Yuan
Source:
Theoretical and applied genetics 2022 v.135 no.9 pp. 3223-3235
ISSN:
0040-5752
Subject:
DNA; Gossypium hirsutum; chromosome mapping; chromosomes; gene expression; genetic background; genetic improvement; heterosis; heterozygosity; lint cotton; nucleotide sequences; phenotypic variation
Abstract:
... KEY MESSAGE : In this study, we present AAQSP as an extension of existing NGS-BSA applications for identifying stable QTLs at high resolution. GhPAP16 and GhIQD14 fine mapped on chromosome D09 of upland cotton are identified as important candidate genes for lint percentage (LP). Bulked segregant analysis combined with next generation sequencing (NGS-BSA) allows rapid identification of genome seque ...
DOI:
10.1007/s00122-022-04181-1

69. AAV infection of bovine embryos: Novel, simple and effective tool for genome editing

Author:
Anna S. Krivonogova; Alexandra V. Bruter; Valeria A. Makutina; Yuliya D. Okulova; Leonid A. Ilchuk; Marina V. Kubekina; Alexandra Yu Khamatova; Tatiana V. Egorova; Vladimir S. Mymrin; Yuliya Yu Silaeva; Alexey V. Deykin; Maxim A. Filatov; Albina G. Isaeva
Source:
Theriogenology 2022 v.193 pp. 77-86
ISSN:
0093-691X
Subject:
DNA; animal reproduction; blastocyst; cattle; genetically modified organisms; genome; lectins; macrophages; serotypes
Abstract:
... Adeno-associated viruses (AAV) are widely used in the field of genetically modified organism production. In this work, transduction of bovine embryos by AAV was selected as a potential approach to perform genetic modifications: we have used recombinant AAV to produce GFP-positive bovine embryos. Five different AAV serotypes were used to evaluate their ability to deliver genetic material into the b ...
DOI:
10.1016/j.theriogenology.2022.09.007

70. The ACTN3 R577X polymorphism is associated with metabolic alterations in a sex‐dependent manner in subjects from western Mexico

Author:
Elisa Barrón‐Cabrera; Nathaly Torres‐Castillo; Karina González‐Becerra; Eloy A. Zepeda‐Carrillo; Rafael Torres‐Valadez; Iván Hernández‐Cañaveral; Erika Martínez‐López
Source:
Journal of human nutrition and dietetics 2022 v.35 no.4 pp. 713-721
ISSN:
0952-3871
Subject:
DNA; bioelectrical impedance; body composition; chemistry; computer software; cross-sectional studies; dietetics; genes; genotype; genotyping; glucose; hypertriglyceridemia; insulin resistance; lifestyle; metabolism; muscles; skeletal muscle; triacylglycerols; Mexico
Abstract:
... BACKGROUND: The ACTN3 gene is primarily expressed in fast skeletal muscle fibres. A common nonsense polymorphism in this gene is ACTN3 R577X (rs1815739), which causes an absolute deficiency of α‐actinin‐3 protein and alterations in muscle metabolism. Considering metabolic alterations are influenced by nutrition and genetic factors, as well as lifestyle factors, we hypothesise a possible associatio ...
DOI:
10.1111/jhn.12948

71. AGAMEMNON: an Accurate metaGenomics And MEtatranscriptoMics quaNtificatiON analysis suite

Author:
Giorgos Skoufos; Fatemeh Almodaresi; Mohsen Zakeri; Joseph N. Paulson; Rob Patro; Artemis G. Hatzigeorgiou; Ioannis S. Vlachos
Source:
Genome biology 2022 v.23 no.1 pp. 39
ISSN:
1474-760X
Subject:
DNA; RNA; data collection; genome; host specificity; metagenomics; transcriptomics
Abstract:
... We introduce AGAMEMNON (https://github.com/ivlachos/agamemnon) for the acquisition of microbial abundances from shotgun metagenomics and metatranscriptomic samples, single-microbe sequencing experiments, or sequenced host samples. AGAMEMNON delivers accurate abundances at genus, species, and strain resolution. It incorporates a time and space-efficient indexing scheme for fast pattern matching, en ...
DOI:
10.1186/s13059-022-02610-4

72. AKT1 mediates multiple phosphorylation events that functionally promote HSF1 activation

Author:
Wen‐Cheng Lu; Ramsey Omari; Haimanti Ray; John Wang; Imade Williams; Curteisha Jacobs; Natasha Hockaden; Matthew L. Bochman; Richard L. Carpenter
Source:
FEBS journal 2022 v.289 no.13 pp. 3876-3893
ISSN:
1742-464X
Subject:
DNA; genes; heat shock proteins; heat shock response; heat stress; mass spectrometry; phosphorylation; phosphotransferases (kinases); proteome; transcription (genetics); transcription factors; transcriptional activation
Abstract:
... The heat stress response activates the transcription factor heat shock factor 1 (HSF1), which subsequently upregulates heat shock proteins to maintain the integrity of the proteome. HSF1 activation requires nuclear localization, trimerization, DNA binding, phosphorylation and gene transactivation. Phosphorylation at S326 is an important regulator of HSF1 transcriptional activity. Phosphorylation a ...
DOI:
10.1111/febs.16375

73. APOBEC3C, a nucleolar protein induced by genotoxins, is excluded from DNA damage sites

Author:
Daniel Constantin; Gilles Dubuis; María del Carmen Conde‐Rubio; Christian Widmann
Source:
FEBS journal 2022 v.289 no.3 pp. 808-831
ISSN:
1742-464X
Subject:
DNA; DNA damage; DNA repair; apolipoprotein B; cytidine deaminase; enzyme activity; etoposide; genome; homologous recombination; humans; immunity; mutagens
Abstract:
... The human genome contains 11 APOBEC (apolipoprotein B mRNA editing catalytic polypeptide‐like) cytidine deaminases classified into four families. These proteins function mainly in innate antiviral immunity and can also restrict endogenous retrotransposable element multiplication. The present study focuses on APOBEC3C (A3C), a member of the APOBEC3 subfamily. Some APOBEC3 proteins use their enzymat ...
DOI:
10.1111/febs.16202

74. ASF -survivors’ sera do not inhibit African swine fever virus replication in vitro

Author:
Marek Walczak; Małgorzata Juszkiewicz; Krzesimir Szymankiewicz; Anna Szczotka-Bochniarz; Grzegorz Woźniakowski
Source:
Journal of Veterinary Research 2022 v.66 no.1 pp. 21-27
ISSN:
2450-8608
Subject:
African swine fever; African swine fever virus; DNA; antibodies; blood serum; cell-mediated immunity; culture media; cytopathogenicity; genotype; growth models; light microscopes; macrophages; neutralization; quantitative polymerase chain reaction; risk; swine; vaccines; veterinary medicine; virus replication; viruses; wild boars; Europe
Abstract:
... African swine fever virus (ASFV) causes one of the most dangerous diseases of pigs and wild boar – African swine fever (ASF). Since its second introduction into Europe (in 2007), the disease has been spreading consistently, and now ASF-free European countries are at risk. Complex interactions between the host’s immune system and the virus have long prevented the development of a safe vaccine again ...
DOI:
10.2478/jvetres-2022-0016

75. ASFV Gene A151R Is Involved in the Process of Virulence in Domestic Swine

Author:
Elizabeth Ramirez-Medina; Elizabeth Vuono; Sarah Pruitt; Ayushi Rai; Nallely Espinoza; Alyssa Valladares; Edward Spinard; Ediane Silva; Lauro Velazquez-Salinas; Douglas P. Gladue; Manuel V. Borca
Source:
Viruses 2022 v.14 no.8 pp. -
ISSN:
1999-4915
Subject:
African swine fever virus; DNA; etiological agents; genes; macrophages; pandemic; swine; viral nonstructural proteins; virulence; virus replication; viruses; Asia; Central European region; Dominican Republic; Georgia; Haiti
Abstract:
... African swine fever virus (ASFV) is the etiological agent of a swine pandemic affecting a large geographical area extending from Central Europe to Asia. The viral disease was also recently identified in the Dominican Republic and Haiti. ASFV is a structurally complex virus with a large dsDNA genome that encodes for more than 150 genes. Most of these genes have not been experimentally characterized ...
DOI:
10.3390/v14081834

76. ATP Hydrolysis Coordinates the Activities of Two Motors in a Dimeric Chromatin Remodeling Enzyme

Author:
Stephanie L. Johnson; Geeta J. Narlikar
Source:
Journal of molecular biology 2022 v.434 no.14 pp. 167653
ISSN:
0022-2836
Subject:
DNA; dissection; genome; hydrolysis; molecular biology; nucleosomes
Abstract:
... ATP-dependent chromatin remodelers are essential enzymes that restructure eukaryotic genomes to enable all DNA-based processes. The diversity and complexity of these processes arethe complexity of the enzymes that carry them out, making remodelers a challenging class of molecular motors to study by conventional methods. Here we use a single molecule biophysical assay to overcome some of these chal ...
DOI:
10.1016/j.jmb.2022.167653

77. ATP Purinergic Receptor P2X1-Dependent Suicidal NETosis Induced by Cryptosporidium parvum under Physioxia Conditions

Author:
Seyed Sajjad Hasheminasab; Iván Conejeros; Zahady D. Velásquez; Tilman Borggrefe; Ulrich Gärtner; Faustin Kamena; Anja Taubert; Carlos Hermosilla
Source:
Biology 2022 v.11 no.3 pp. -
ISSN:
2079-7737
Subject:
Cryptosporidium parvum; DNA; Protozoa; acidification; cattle; cryptosporidiosis; elastase; extrusion; fluorescence microscopy; glucose; glycolysis; helminths; hyperoxia; intestines; lactic acid; neutrophils; oocysts; oxygen; oxygen consumption; plasma membrane; purinergic receptors; sporozoites; wildlife
Abstract:
... Cryptosporidiosis is a zoonotic intestinal disease that affects humans, wildlife, and neonatal cattle, caused by Cryptosporidium parvum. Neutrophil extracellular traps (NETs), also known as suicidal NETosis, are a powerful and ancient innate effector mechanism by which polymorphonuclear neutrophils (PMN) battle parasitic organisms like protozoa and helminths. Here, C. parvum oocysts and live sporo ...
DOI:
10.3390/biology11030442

78. ATP-Responsive Strand Displacement Coupling with DNA Origami/AuNPs Strategy for the Determination of Microcystin-LR Using Surface-Enhanced Raman Spectroscopy

Author:
Bingyang Huo; Ling Xia; Zhixian Gao; Gongke Li; Yuling Hu
Source:
Analytical chemistry 2022 v.94 no.34 pp. 11889-11897
ISSN:
1520-6882
Subject:
DNA; Raman spectroscopy; adenosine triphosphate; analytical chemistry; detection limit; liquid chromatography; microcystin-LR; nanomaterials; oligonucleotides; solid phase extraction; tandem mass spectrometry
Abstract:
... The DNA origami-mediated self-assembly strategy has emerged as a powerful tool in surface-enhanced Raman spectroscopy (SERS). However, these self-assembly approaches typically do not possess high detection specificity. Herein, a novel strategy based on adenosine triphosphate (ATP)-responsive strand displacement (ARSD) coupling with DNA origami/AuNPs for SERS analysis of microcystin-LR (MC-LR) is p ...
DOI:
10.1021/acs.analchem.2c02440

79. ATP-Triggered Drug Release of Self-Assembled 3D DNA Nanostructures for Fluorescence Imaging and Tumor Therapy

Author:
Yao Jiang; Huimin Zhou; Wenjing Zhao; Shusheng Zhang
Source:
Analytical chemistry 2022 v.94 no.18 pp. 6771-6780
ISSN:
1520-6882
Subject:
DNA; analytical chemistry; drugs; fluorescence; medicine; nanocarriers; neoplasms
Abstract:
... Stimulus-responsive materials are ideal carriers for precisely controlled drug delivery due to their high selectivity. However, the complex physiological environment hinders its development in clinical medicine. Here, we aim to design a self-assembled three-dimensional (3D) DNA nanostructure drug delivery system with adenosine-5’-triphosphate (ATP)-triggered drug release for tumor fluorescence ima ...
DOI:
10.1021/acs.analchem.2c00409

80. ATR-FTIR spectroscopy as a pre-screening technique for the PMI assessment and DNA preservation in human skeletal remains – A review

Author:
Leskovar Tamara; Zupanič Pajnič Irena; Jerman Ivan; Črešnar Matija
Source:
Quaternary international 2022 pp. -
ISSN:
1040-6182
Subject:
DNA; archaeology; chemometrics; collagen; forensic sciences; humans; postmortem changes; prediction; spectroscopy
Abstract:
... Skeletal remains from archaeological and forensic contexts are commonly used for various analyses, including DNA. Our research investigates relatively fast and inexpensive ATR-FTIR spectroscopy with further data manipulation as a potential pre-screening technique for assessing the preservation state of skeletal remains. We seek to correlate spectra and chemometric indices with the biological, chem ...
DOI:
10.1016/j.quaint.2022.09.004

81. AZD1222 (ChAdOx1 nCov-19): A Single-Dose biodistribution study in mice

Author:
Richard Stebbings; Gillian Armour; Vivian Pettis; Joanne Goodman
Source:
Vaccine 2022 v.40 no.2 pp. 192-195
ISSN:
0264-410X
Subject:
COVID-19 infection; DNA; blood; brain; intramuscular injection; nerve tissue; particulates; reticuloendothelial system; spinal cord; vaccines
Abstract:
... Biodistribution studies of adenovirus-based vaccines support their clinical development by evaluating their spread and persistence following in vivo administration. AZD1222 (ChAdox1 nCov-19) is a replication-deficient non-human adenovirus-vectored vaccine for coronavirus disease 2019. In this nonclinical study, the biodistribution of AZD1222 was assessed in mice for 29 days following intramuscular ...
DOI:
10.1016/j.vaccine.2021.11.028

82. Abiotic stress triggers ROS-mediated programmed cell death in banana (Musa sp., AAA group, Cavendish sub-group) fruit

Author:
Maricruz Ramírez-Sánchez; Donald J. Huber; C. Eduardo Vallejos
Source:
Scientia horticulturae 2022 v.293 pp. 110748
ISSN:
0304-4238
Subject:
DNA; Musa; abiotic stress; banana peels; bananas; cell structures; enzyme activity; ethylene; fruits; homeostasis; metabolism; programmed cell death
Abstract:
... Degradative processes of fleshy fruit adapt depending on environmental conditions. Exposure of banana fruit to abiotic stress during ripening accelerates degradative processes that resemble Programmed Cell Death (PCD). This research evaluated the connection between Reactive Oxygen Species (ROS) production and PCD processes during ripening and over-ripening of banana fruit after exposure to abiotic ...
DOI:
10.1016/j.scienta.2021.110748

83. Abortion and congenital transmission of Trypanosoma vivax in goats and ewes in semiarid northeastern Brazil

Author:
Jael Soares Batista; Wanderson Lucas Alves dos Santos; Ana Caroline Freitas Caetano de Sousa; Tiago da Silva Teófilo; Ana Carla Diógenes Suassuna Bezerra; Victor Hugo Vieira Rodrigues; José Ailton da Silva Filho; Tânia Vasconcelos Cavalcante; Kizzy Millenn de Freitas Mendonça Costa; Geysa Almeida Viana
Source:
Research in veterinary science 2022 v.149 pp. 125-127
ISSN:
0034-5288
Subject:
DNA; Trypanosoma vivax; blood; parasitemia; parasites; polymerase chain reaction; research; semiarid zones; trypanosomiasis; trypomastigotes; veterinary medicine; Brazil
Abstract:
... The main effects of trypanosomosis in Brazil are related to reproductive alterations. In this context, the present study aimed to report the occurrence of abortions in goats and sheeps in the semiarid region of Northeastern Brazil, associated with Trypanosoma vivax. Trypomastigotes forms visualized by Buffy coat technique (BCT) method in 68.7% of the goats and 50.0% of the ewes that aborted. PCR i ...
DOI:
10.1016/j.rvsc.2022.06.009

84. Absence of proteinase K‐resistant PrP in Korean Holstein cattle carrying potential bovine spongiform encephalopathy‐related E211K somatic mutation

Author:
Yong‐Chan Kim; Sae‐Young Won; Min‐Ju Jeong; Byung‐Hoon Jeong
Source:
Transboundary and emerging diseases 2022 v.69 no.2 pp. 805-812
ISSN:
1865-1674
Subject:
DNA; Holstein; alleles; bovine spongiform encephalopathy; cattle; females; germ cells; haplotypes; linkage disequilibrium; males; meat; medulla oblongata; monitoring; prions; proteinases; somatic mutation; Korean Peninsula
Abstract:
... Bovine spongiform encephalopathy (BSE) is a kind of prion disease caused by proteinase K‐resistant prion protein (PrPSᶜ) in cattle. Although BSE has been reported worldwide, BSE‐infected cases have never been reported in Korea. In a previous study, we identified BSE‐related somatic mutation E211K in 3 Korean Holstein cattle. In Korea, the BSE surveillance system has been established. However, seve ...
DOI:
10.1111/tbed.14053

85. Acacetin antagonized lipotoxicity in pancreatic β-cells via ameliorating oxidative stress and endoplasmic reticulum stress

Author:
Ning Wang; Qing Gao; Jie Shi; Chen Yulan; Weimeng Ji; Xiumei Sheng; Rui Zhang
Source:
Molecular biology reports 2022 v.49 no.9 pp. 8727-8740
ISSN:
0301-4851
Subject:
DNA; antioxidant activity; apoptosis; biomarkers; calcium; cell viability; diabetes; endoplasmic reticulum; endoplasmic reticulum stress; free fatty acids; lipotoxicity; molecular biology; oxidative stress; palmitic acid; pathogenesis; pro-apoptotic proteins; protein synthesis; reactive oxygen species
Abstract:
... PURPOSE: During the pathogenesis and progression of diabetes, lipotoxicity is a major threat to the function and survival of pancreatic β-cells. To battle against the lipotoxicity induced cellular damages, the present study investigated the beneficial effects of acacetin, a natural antioxidant, on free fatty acid (FFA) stressed RINm5F cells and the potential mechanism involved. MATERIALS AND METHO ...
DOI:
10.1007/s11033-022-07717-2

86. Accelerated cycling PCR: A novel tool for rapid, sensitive and specific detection of single-nucleotide mutation within 30 min

Author:
Zhixian Luan; Yan Zhao; Yanling Wang; Cuiping Ma; Chao Shi
Source:
Journal of microbiological methods 2022 v.199 pp. 106527
ISSN:
0167-7012
Subject:
DNA; Helicobacter pylori; drug resistance; genotype; genotyping; mutation; rapid methods
Abstract:
... Rapid detection of single-nucleotide mutations (SNMs) has played a vital role for point-of-care testing. We herein first introduced accelerated thermal cycling into conventional allele-specific qPCR (AS-qPCR), named accelerated cycling PCR (AC-PCR) to achieve rapid and sensitive detection of SNM. It could simultaneously detect 10 copies of H. pylori DNA and identify its clarithromycin-resistance g ...
DOI:
10.1016/j.mimet.2022.106527

87. Accelerated evolution of Vkorc1 in desert rodent species reveals genetic preadaptation to anticoagulant rodenticides

Author:
Yan Chen; Dawei Wang; Ning Li; Deng Wang; Xiao‐Hui Liu; Ying Song
Source:
Pest management science 2022 v.78 no.6 pp. 2704-2713
ISSN:
1526-498X
Subject:
DNA; Psammomys obesus; amino acids; anticoagulant rodenticides; anticoagulants; blood coagulation; endoplasmic reticulum; models; natural selection; quinones; rodents; vitamin K epoxide reductase
Abstract:
... BACKGROUND: Some rodent species living in arid areas show elevated physiological tolerance to anti‐vitamin K rodenticides (AVKs), which seems to be due to some unknown selective pressures that rodents may experience in desert habitats. Genes involved in the ϒ‐carboxylation of blood coagulation, including vitamin K epoxide reductase complex, subunit 1 (Vkorc1), ϒ‐glutamyl‐carboxylase (Ggcx) and NAD ...
DOI:
10.1002/ps.6905

88. Access and benefit‐sharing DNA Componentry for plant synthetic biology: Bioparts expressed in plant chassis

Author:
Michelle Rourke
Source:
Plants, people, planet 2022 v.4 no.1 pp. 76-83
ISSN:
2572-2611
Subject:
DNA; genetic resources; synthetic biology
Abstract:
... SOCIETAL IMPACT STATEMENT: The “Parts Agenda” is an approach to synthetic biology that fragments genetic resources into functional bioparts to help design and build biological devices and systems. Access and benefit‐sharing (ABS), and the issue of how to regulate digital sequence information (DSI) within the current ABS regime, poses a problem for synthetic biology because it assumes fragmented an ...
DOI:
10.1002/ppp3.10184

89. Acclimation of elk mating system following restoration to the Missouri Ozarks, U.S.A.

Author:
Ellen M. Pero; M. Colter Chitwood; Aaron M. Hildreth; Leah K. Berkman; Barbara J. Keller; Jason A. Sumners; Lonnie P. Hansen; Jason L. Isabelle; Lori S. Eggert; Chelsea L. Titus; Joshua J. Millspaugh
Source:
Restoration ecology 2022 v.30 no.7 pp. e13623
ISSN:
1061-2971
Subject:
Cervus canadensis; DNA; acclimation; age structure; ecological restoration; elks; genetic variation; males; paternity; polygyny; population density; reproductive success; sires; Kentucky; Missouri; Ozarks
Abstract:
... Biologists increasingly use translocation to restore animals to areas where they have been extirpated. However, we know little about how translocation decisions influence translocation‐mediated social dynamics. Breeding in polygynous ungulate mating systems is typically dominated by prime age males, but founding males within translocated ungulate populations often are comprised of only young indiv ...
DOI:
10.1111/rec.13623

90. Accumulation of polystyrene microplastics induces liver fibrosis by activating cGAS/STING pathway

Author:
Rong Shen; Keren Yang; Xin Cheng; Changlei Guo; Xiqing Xing; Haonan Sun; Disheng Liu; Xiangwen Liu; Degui Wang
Source:
Environmental pollution 2022 v.300 pp. 118986
ISSN:
0269-7491
Subject:
DNA; DNA damage; cytokines; fibronectins; fibrosis; hepatocytes; liver; liver cirrhosis; microplastics; mitochondria; pollution; polystyrenes; risk; toxicity
Abstract:
... The environmental pollution from microplastics has caused concern from the whole society due to its harm to organisms. However, the effect of microplastics on liver damage and fibrosis remains unclear in the case of long-term accumulation. The present study demonstrated that the 0.1 μm microplastic could enter hepatocytes from circulation and result liver damage even at a low concentration. Microp ...
DOI:
10.1016/j.envpol.2022.118986

91. Accuracy of genotype imputation to whole genome sequencing level using different populations of Nile tilapia

Author:
Baltasar F. Garcia; Grazyella M. Yoshida; Roberto Carvalheiro; José M. Yáñez
Source:
Aquaculture 2022 v.551 pp. 737947
ISSN:
0044-8486
Subject:
DNA; Oreochromis niloticus; aquaculture; computer software; cost effectiveness; genome; genomics; genotype; quality control
Abstract:
... A cost-effective strategy to obtain ultra-dense genomic information is to sequence part of population and perform imputation from lower density genotypes to sequence level for the remaining animals. The aims of this study were to evaluate the feasibility of genotype imputation from medium density to sequence level in Nile tilapia and to investigate the impacts of size and origin of reference popul ...
DOI:
10.1016/j.aquaculture.2022.737947

92. Accurate Cultivar Authentication of Jujube Fruits Using Nano-Fluidic Genotyping of Single Nucleotide Polymorphism (SNP) Markers

Author:
Yue Zhang; Yaping Ma; Lyndel W. Meinhardt; Dapeng Zhang; Bing Cao; Lihua Song
Source:
Horticulturae 2022 v.8 no.9 pp. -
ISSN:
2311-7524
Subject:
DNA; Ziziphus jujuba; adulterated products; barcoding; climate; cost effectiveness; cultivars; flavor; fruit pulp; fruit trees; fruits; genotyping; industry; market prices; markets; ordination techniques; single nucleotide polymorphism; traceability; China
Abstract:
... Chinese jujube (Ziziphus jujuba Mill.) is an economically important fruit tree that is highly adapted to marginal crop lands and can be grown under a very broad range of climate conditions. Jujube fruits produced from several traditional cultivars in Ningxia, China have enjoyed a premium market price due to their unique flavor and quality attributes. One problem with the high-end jujube market is ...
DOI:
10.3390/horticulturae8090792

93. Accurate, rapid and highly sensitive detection of African swine fever virus via graphene oxide-based accelerated strand exchange amplification

Author:
Linlin Zhuang; Jianbo Yang; Chunlei Song; Li Sun; Bin Zhao; Qiuping Shen; Xiyan Ren; Hongjing Shi; Yu Zhang; Mengling Zhu
Source:
Analytical methods 2022 v.14 no.21 pp. 2072-2082
ISSN:
1759-9679
Subject:
African swine fever; African swine fever virus; DNA; detection limit; graphene; nose; point-of-care testing; pork industry; swine
Abstract:
... African swine fever is an acute, severe and highly contagious infectious disease caused by African swine fever virus (ASFV), posing a huge threat to the global swine industry. Rapid and accurate diagnostic methods are of great significance for the effective prevention and control of ASFV transmission. In this work, we established and evaluated a graphene oxide-based accelerated strand exchange amp ...
DOI:
10.1039/d2ay00610c

94. Acetylation of Sesquiterpenyl Epoxy-Cyclohexenoids Regulates Fungal Growth, Stress Resistance, Endocytosis, and Pathogenicity of Nematode-Trapping Fungus Arthrobotrys oligospora via Metabolism and Transcription

Author:
Yong-Hong Chen; Long-Long Zhang; Li-Jun Wang; Xu-Tong Yue; Qun-Fu Wu; Yang Jiang; Ke-Qin Zhang; Xue-Mei Niu
Source:
Journal of agricultural and food chemistry 2022 v.70 no.20 pp. 6145-6155
ISSN:
1520-5118
Subject:
Arthrobotrys oligospora; DNA; acetylation; autophagosomes; biosynthesis; endocytosis; exosomes; food chemistry; fungal growth; glutamine; mutants; nematophagous fungi; pathogenicity; phenylpropanoids; polyketides; soil; stress tolerance; transcription (genetics); viruses
Abstract:
... Sesquiterpenyl epoxy-cyclohexenoids (SECs) that depend on a polyketide synthase–terpenoid synthase (PKS–TPS) pathway are widely distributed in plant pathogenic fungi. However, the biosynthesis and function of the acetylated SECs still remained cryptic. Here, we identified that AOL_s00215g 273 (273) was responsible for the acetylation of SECs in Arthrobotrys oligospora via the construction of Δ273, ...
DOI:
10.1021/acs.jafc.2c01914

95. Achieving maximum efficiency of Mungbean yellow mosaic India virus infection in mungbean by agroinoculation

Author:
Palaiyur N. Sivalingam; Narasimham Dokka; Mahesh M. Mahajan; Bhimeshwari Sahu; Ashish Marathe; Pankaj Kaushal; Probir Kumar Ghosh
Source:
3 Biotech 2022 v.12 no.1 pp. 29
ISSN:
2190-572X
Subject:
Agrobacterium; Aleyrodidae; DNA; Mungbean yellow mosaic India virus; Mungbean yellow mosaic virus; acetosyringone; genomics; germplasm; leaves; meristems; mung beans; pathogenicity; soil
Abstract:
... Mungbean is one of the important food legumes in the Indian–sub-continent. Yellow mosaic disease, caused by Mungbean yellow mosaic virus and Mungbean yellow mosaic India virus (MYMIV) poses a severe threat to its production. Agroinoculation has been the most preferred way to test the function of genomic components of these viruses. However, the available inoculation methods are not as efficient as ...
DOI:
10.1007/s13205-021-03088-w

96. Achieving robust somatic mutation detection with deep learning models derived from reference data sets of a cancer sample

Author:
Sayed Mohammad Ebrahim Sahraeian; Li Tai Fang; Konstantinos Karagiannis; Malcolm Moos; Sean Smith; Luis Santana-Quintero; Chunlin Xiao; Michael Colgan; Huixiao Hong; Marghoob Mohiyuddin; Wenming Xiao
Source:
Genome biology 2022 v.23 no.1 pp. 12
ISSN:
1474-760X
Subject:
DNA; DNA damage; cell lines; computer simulation; gene frequency; genome; genomics; models; neoplasm cells; neoplasms; somatic mutation
Abstract:
... BACKGROUND: Accurate detection of somatic mutations is challenging but critical in understanding cancer formation, progression, and treatment. We recently proposed NeuSomatic, the first deep convolutional neural network-based somatic mutation detection approach, and demonstrated performance advantages on in silico data. RESULTS: In this study, we use the first comprehensive and well-characterized ...
DOI:
10.1186/s13059-021-02592-9

97. Acid adaptive response of Alicyclobacillus acidoterrestris: A strategy to survive lethal heat and acid stresses

Author:
Ning Zhao; Junnan Xu; Lingxia Jiao; Miaomiao Liu; Tong Zhang; Jun Li; Xinyuan Wei; Mingtao Fan
Source:
Food research international 2022 v.157 pp. 111364
ISSN:
0963-9969
Subject:
Alicyclobacillus acidoterrestris; DNA; DNA repair; Fourier transform infrared spectroscopy; acid tolerance; biosynthesis; cell membranes; decarboxylation; fatty acids; food research; fruit juices; gene expression; gene expression regulation; glutamic acid; heat; homeostasis; homologous recombination; juice industry; pH; pasteurization; phospholipids; roughness; shrinkage; spoilage; sporulation; transcriptomics; urease; zwitterions
Abstract:
... Alicyclobacillus acidoterrestris causes the spoilage of pasteurized acidic fruit juice, seriouslydecreasing quality and posing a significant safety concern. We previously discovered that acid adaptation could induce stress adaptive responses of A. acidoterrestris, however, the underlying mechanisms of this induction have not been fully elucidated. In this work, the effects of acid adaptation (pH = ...
DOI:
10.1016/j.foodres.2022.111364

98. Acid-Resistant and Physiological pH-Responsive DNA Hydrogel Composed of A-Motif and i-Motif toward Oral Insulin Delivery

Author:
Yuwei Hu; Shujun Gao; Hongfang Lu; Jackie Y. Ying
Source:
Journal of the American Chemical Society 2022 v.144 no.12 pp. 5461-5470
ISSN:
1520-5126
Subject:
DNA; acid tolerance; acrylamides; adenine; composite polymers; crosslinking; cytosine; dissociation; drugs; duodenum; electrostatic interactions; hydrogels; insulin; oligonucleotides; pH; polymerization; protonation; stomach
Abstract:
... An acid-resistant DNA hydrogel that is stable in an extremely acidic environment with pH as low as 1.2 has not been reported before, largely due to the instability of DNA-hybridized structures. To achieve this, adenine (A)-rich and cytosine (C)-rich oligonucleotides are rationally designed and integrated to form copolymers with acrylamide monomers via free-radical polymerization. In an acidic envi ...
DOI:
10.1021/jacs.1c13426

99. Aconitine induces autophagy via activating oxidative DNA damage-mediated AMPK/ULK1 signaling pathway in H9c2 cells

Author:
Wenlin Wang; Jialuo Jiang; Yan Huang; Fu Peng; Tingting Hu; Jiayang Wu; Xiaoqi Pan; Chaolong Rao
Source:
Journal of ethnopharmacology 2022 v.282 pp. 114631
ISSN:
0378-8741
Subject:
Aconitum; DNA; DNA damage; aconitine; antioxidants; arrhythmia; autophagosomes; autophagy; cardiomyocytes; cardiotoxicity; cell viability; confocal microscopy; death; enzyme-linked immunosorbent assay; fibrosis; flow cytometry; phosphorylation; protein synthesis; rheumatoid arthritis; toxicity; traditional medicine
Abstract:
... Aconitum species, with a medicinal history of 2000 years, was traditionally used in the treatment of rheumatism, arthritis, bruises, and pains. However, many studies have reported that Aconitum species can cause arrhythmia in experimental animals, resulting in myocardial fibrosis and cardiomyocyte damage. Cardiotoxicity is the main toxic effect of aconitine, but the detailed mechanism remains uncl ...
DOI:
10.1016/j.jep.2021.114631

100. Actin Contributes to the Hyperexpression of Baculovirus Polyhedrin (polh) and p10 as a Component of Transcription Initiation Complex (TIC)

Author:
Nan Chen; Guanping Chen; Xiangshuo Kong; Xiaofeng Wu
Source:
Viruses 2022 v.14 no.1 pp. -
ISSN:
1999-4915
Subject:
Baculoviridae; DNA; DNA-directed RNA polymerase; actin; chromatin immunoprecipitation; genetic vectors; luciferase; physiological transport; transcription initiation
Abstract:
... Hyperexpression of polh and p10, two very late genes, is one of the remarkable characteristics in the baculovirus life cycle. However, the mechanisms underlying the hyperexpression of these two genes are still incompletely understood. In this study, actin was identified as a highly potential binding partner of polh and p10 promoters by conducting DNA pull-down and LC–MS/MS analyses. Inhibiting act ...
DOI:
10.3390/v14010153