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1. 1-Methylcyclopropene (1-MCP) treatment differentially mediated expression of vacuolar processing enzyme (VPE) genes and delayed programmed cell death (PCD) during ripening and senescence of apple fruit

Author:
Jingyi Lv; Yingzhi Zhang; Mingyu Sun; Jingxin Chen; Yonghong Ge; Jianrong Li
Source:
Scientia horticulturae 2023 v.307 pp. 111489
ISSN:
0304-4238
Subject:
1-methylcyclopropene; DNA; Malus domestica; apples; climacteric fruits; electrical conductivity; enzymes; ethylene; firmness; malondialdehyde; programmed cell death; reactive oxygen species; transmission electron microscopy; vacuoles
Abstract:
... The main purpose of this research was to evaluate the impacts of 1-methylcyclopropene (1-MCP) treatment on vacuolar processing enzyme (VPE) genes and programmed cell death (PCD) during ripening and senescence of apple fruit (Malus × domestica Borkh.). The ethylene antagonists 1-MCP (1 μL L⁻¹) was applied to apples after harvest. Both treated and control fruit were stored at 20 ± 2°C. The data show ...
DOI:
10.1016/j.scienta.2022.111489

2. Anchoring red blood cell with tetrahedral DNA nanostructure: Electrochemical biosensor for the sensitive signage of circulating tumor DNA

Author:
Xinyue Zha; Weiwei Qin; Jiadi Chen; Mengting Chen; Qiyue Zhang; Kaiyu He; Yingju Liu; Weipeng Liu
Source:
Talanta 2023 v.251 pp. 123793
ISSN:
0039-9140
Subject:
DNA; biocompatibility; biosensors; blood serum; detection limit; erythrocytes; methylene blue; neoplasms; rapid methods
Abstract:
... Circulating tumor cells (CTCs), as a type of tumor, have attracted wide attention because of their characteristics of shedding from the primary tumor and spreading to other tissues and organs through peripheral blood. The circulating tumor DNA (ctDNA), the DNA released by CTCs and other tumor cells into the peripheral blood, was considered as a promising detection substance for clinical applicatio ...
DOI:
10.1016/j.talanta.2022.123793

3. Capsalid monogeneans of fishes from the Seto Inland Sea, Japan: Description of Benedenia kobudai n. sp. parasitic on Semicossyphus reticulatus (Perciformes: Labridae)

Author:
Masato Nitta
Source:
Parasitology international 2023 v.92 pp. 102677
ISSN:
1383-5769
Subject:
Capsalidae; DNA; Japan; Semicossyphus; aquaculture; biodiversity; fish; fisheries; host range; new species; parasitology; penis; phylogeny
Abstract:
... The Seto Inland Sea, the largest inland sea in Japan, is a rich fishery with high biodiversity and productivity. Monogeneans have been studied for more than 120 years, and 58 nominal species have been recorded in the Seto inland Sea. This study provided DNA information on five species of Benedenia sensu lato (Capsalidae) from marine fishes from the sea, and one of them, Benedenia kobudai n. sp., i ...
DOI:
10.1016/j.parint.2022.102677

4. Cascaded molecular logic gates using antibiotics as inputs based on exonuclease III and DNAzyme

Author:
Fang Deng; Jiafeng Pan; Zhi Liu; Junhua Chen
Source:
Talanta 2023 v.252 pp. 123832
ISSN:
0039-9140
Subject:
DNA; analytical methods; catalytic activity; chloramphenicol; fluorescence; kanamycin; nucleic acid hybridization; oxytetracycline
Abstract:
... We successfully constructed several cascaded molecular logic gates (2INHIBIT-2AND, 2AND-2OR, and 2OR-2INHIBIT) using three different antibiotics as the inputs. In the presence of kanamycin (KAN), chloramphenicol (CHL), or oxytetracycline (OXY), the aptamer-antibiotic recognition will release the trigger DNA to active the hairpin DNA hybridization. Exonuclease III (Exo III)-mediated catalysis react ...
DOI:
10.1016/j.talanta.2022.123832

5. Characterization and functional analysis of chicken dsRNA binding protein hnRNPU

Author:
Xifeng Hu; Xiangdong Wu; Zhen Ding; Zheng Chen; Huansheng Wu
Source:
Developmental and comparative immunology 2023 v.138 pp. 104521
ISSN:
0145-305X
Subject:
DNA; RNA; Vesiculovirus; chickens; cytosol; genomics; humans; immunology; nuclear matrix; ribonucleoproteins
Abstract:
... In mammals, heterogeneous ribonucleoprotein U (hnRNPU), also named as nuclear matrix protein-nuclear scaffold attachment factor (SAFA), was originally identified as a DNA/RNA interactor protein. It has been reported that human hnRNPU facilitates IFN-β generation after vesicular stomatitis virus (VSV) infection. Nevertheless, the role of chicken hnRNPU (chhnRNPU) in IFN-β regulation as well as in i ...
DOI:
10.1016/j.dci.2022.104521

6. Dietary fiber konjac glucomannan exerts an antidiabetic effect via inhibiting lipid absorption and regulation of PPAR-γ and gut microbiome

Author:
Muthukumaran Jayachandran; Sunil Christudas; Xuchu Zheng; Baojun Xu
Source:
Food chemistry 2023 v.403 pp. 134336
ISSN:
0308-8146
Subject:
absorption; blood lipids; body weight; dietary fiber; drugs; fibrosis; food chemistry; glycemic effect; high fat diet; hyperlipidemia; immunohistochemistry; insulin; intestinal microorganisms; konjac mannan; lipid composition; lipid metabolism; lipids; liver; microbiome; noninsulin-dependent diabetes mellitus; protein synthesis; streptozotocin
Abstract:
... Konjac glucomannan’s influence on the regulation of diabetes mellitus, hyperlipidemia, and gut microbial flora was evaluated in this study. In addition, a high-fat diet and streptozotocin were used to induce type 2 diabetes mellitus in rats. At the end of the study, we analyzed various parameters such as body weight, plasma lipid profile, insulin levels by immunohistochemistry, degree of fibrosis ...
DOI:
10.1016/j.foodchem.2022.134336

7. Dietary tryptophan requirement of fingerling Channa punctatus (Bloch) based on growth, hematological parameters, intestinal enzymes, non-specific immune response, and antioxidant capacity

Author:
Yusra Sharf; Mukhtar A. Khan
Source:
Aquaculture 2023 v.562 pp. 738745
ISSN:
0044-8486
Subject:
Channa punctata; DNA; RNA; alanine transaminase; antioxidant activity; aquaculture; aspartate transaminase; casein; catalase; erythrocyte sedimentation rate; fingerlings; gelatin; glutathione peroxidase; hematocrit; hemoglobin; immune response; intestines; lysozyme; malondialdehyde; regression analysis; satiety; specific growth rate; superoxide dismutase; tryptophan; weight gain
Abstract:
... In this investigation, the tryptophan requirement of C. punctatus fingerling (5.45 ± 0.07 g) was determined by feeding seven isoproteic (450 g kg⁻¹ CP) and isoenergetic (14.7 kJ g⁻¹ DE) diets with graded levels of tryptophan 1, 2, 3, 4, 5, 6, and 10 g kg⁻¹, respectively to randomly stocked groups in triplicate at 8:00, 12:30 and 17:30 h near to satiety for 12 weeks. Tryptophan contributed by casei ...
DOI:
10.1016/j.aquaculture.2022.738745

8. Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement

Author:
Cuiying Lin; Qingqing Huang; Mengjian Tian; Fang Luo; Jian Wang; Bin Qiu; Shuofei Yang; Zhenyu Lin
Source:
Talanta 2023 v.251 pp. 123748
ISSN:
0039-9140
Subject:
DNA; adenine; biosensors; blood serum; detection limit; electrochemiluminescence; electrodes; ferrocenes; indium; methylation; methyltransferases; restriction endonucleases; tin
Abstract:
... In this work, an electrochemiluminescence (ECL) biosensor with dual signal enhancement was constructed and used for DNA adenine methylation methyltransferase (Dam MTase) detection. At present of Dam MTase, restriction endonuclease (DPnI) cleaves hairpin DNA (HP) and releases the HP stem end as a single strand that can activate CRISPR/Cas12a trans-cleavage activity. Assisted by trans-cleavage, the ...
DOI:
10.1016/j.talanta.2022.123748

9. Genome–wide association study and genomic prediction of growth traits in bighead catfish (Clarias macrocephalus Günther, 1864)

Author:
Prapaiphan Chaivichoo; Sila Sukhavachana; Rabuesak Khumthong; Prapansak Srisapoome; Satid Chatchaiphan; Uthairat Na-Nakorn
Source:
Aquaculture 2023 v.562 pp. 738748
ISSN:
0044-8486
Subject:
Clarias macrocephalus; DNA; aquaculture; body weight; catfish; cost effectiveness; genes; genetic improvement; genetic variance; genome-wide association study; genomics; genotyping; heritability; marker-assisted selection; parentage; phenotype; prediction; technology; variance; zinc finger motif
Abstract:
... Next-generation sequencing (NGS) enables cost-effective exploration of genome-wide single nucleotide polymorphisms (SNPs). This new technology has made enormous contributions in various fields including genetic improvement. This study was conducted to identify SNPs that associated with growth traits (body weight, standard length, and total length) of bighead catfish (Clarias macrocephalus Günther, ...
DOI:
10.1016/j.aquaculture.2022.738748

10. High-performance strategy for the construction of electrochemical biosensor for simultaneous detection of miRNA-141 and miRNA-21 as lung cancer biomarkers

Author:
Ali Khodadoust; Navid Nasirizadeh; Seyed Morteza Seyfati; Ramezan Ali Taheri; Mostafa Ghanei; Hasan Bagheri
Source:
Talanta 2023 v.252 pp. 123863
ISSN:
0039-9140
Subject:
DNA; DNA probes; biomarkers; biosensors; glassy carbon electrode; hybridization; lung neoplasms; microRNA; signal-to-noise ratio
Abstract:
... In this study, the dual signal-labeled hairpin-structured DNA (dhDNA)-based probes have been developed to construct a novel nano-biosensor. This one hairpin-structured probe consists of a thiolated methylene blue-labeled hairpin capture probe (MB-HCP) as an inner reference probe and a ferrocene-modified anti-miRNA-21 DNA probe (Fc-AP-21). This novel integrated structure of MB-HCP and Fc-AP-21 was ...
DOI:
10.1016/j.talanta.2022.123863

11. Improved sperm DNA integrity and altered fat metabolism and intestinal morphology in Nile tilapia (Oreochromis niloticus) males fed rations with purified nucleotide

Author:
Robie Allan Bombardelli; Juliano Kelvin dos Santos Henriques; Marta Margarete Cestari; Antônio Ernesto Meister Luz Marques; Taís Voelkl Chagas; Fabio Meurer
Source:
Aquaculture 2023 v.562 pp. 738682
ISSN:
0044-8486
Subject:
DNA; Oreochromis niloticus; aquaculture; blood glucose; chromatin; diet; digestible energy; digestible protein; enterocytes; fish; gonadosomatic index; hepatosomatic index; high density lipoprotein; lipid content; lipid metabolism; liver; morphometry; muscles; nucleotides; testes; triacylglycerols; visceral fat; viscosity
Abstract:
... In this study, we assessed the health, growth, sperm characteristics, and intestinal, liver, and testicular morphology of Nile tilapia (Oreochromis niloticus) males fed rations containing purified nucleotides (NT). A total of 420 females (286 ± 7.15 g) and 140 males (536 ± 13.4 g) were used. Fish were fed on extruded ration (280 g digestible protein kg⁻¹; 11.72 M joule (MJ) of digestible energy kg ...
DOI:
10.1016/j.aquaculture.2022.738682

12. Insights into a novel nitrogen removal process based on simultaneous anammox and denitrification (SAD) following nitritation with in-situ NOB elimination

Author:
Jing Chen; Jia Zeng; Yiran He; Shiquan Sun; Haipeng Wu; Yaoyu Zhou; Zhenguo Chen; Jianhui Wang; Hong Chen
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 160-170
ISSN:
1001-0742
Subject:
Comamonadaceae; DNA; anaerobic ammonium oxidation; batch systems; denitrification; nitrogen; total nitrogen; wastewater; wastewater treatment; zeolites; China
Abstract:
... Simultaneous anammox and denitrification (SAD) is an efficient approach to treat wastewater having a low C/N ratio; however, few studies have investigated a combination of SAD and partial nitritation (PN). In this study, a lab-scale up-flow blanket filter (UBF) and zeolite sequence batch reactor (ZSBR) were continuously operated to implement SAD and PN advantages, respectively. The UBF achieved a ...
DOI:
10.1016/j.jes.2022.01.019

13. Integrated multi-omic analyses on yellow Flammulina filiformis cultivar reveal postharvest oxidative damage responses

Author:
Yu Fu; Hao Tan; Bo Wang; Weihong Peng; Qun Sun; Yang Yu
Source:
Postharvest biology and technology 2023 v.195 pp. 112111
ISSN:
0925-5214
Subject:
DNA; Flammulina; biosynthesis; catechol oxidase; cell senescence; cultivars; genome; laccase; melanin; mushrooms; oxidative phosphorylation; oxygen; proteolysis; proteome; proteomics; technology; transcriptome; transcriptomics; tyrosine
Abstract:
... Yellow cultivars of Flammulina filiformis are prone to browning, which causes significant yearly loss. This study investigated the critical factors involved and revealed that oxygen plays an essential role in the browning process. Integrated multi-omic analyses, including genome sequencing, transcriptome profiling, and 4D label-free proteome quantification, were incorporated to dissect essential g ...
DOI:
10.1016/j.postharvbio.2022.112111

14. Interaction of Polycyclic Aromatic Hydrocarbon compounds in fish primary hepatocytes: From molecular mechanisms to genotoxic effects

Author:
Isabella Bramatti; Beatriz Matos; Neusa Figueiredo; Pedro Pousão-Ferreira; Vasco Branco; Marta Martins
Source:
Science of the total environment 2023 v.855 pp. 158783
ISSN:
0048-9697
Subject:
DNA; DNA damage; Sparus aurata; Western blotting; carcinogenicity; cell viability; chemical structure; comet assay; environment; environmental assessment; fish; genes; genotoxicity; hepatocytes; metabolites; mutagens; phenanthrenes; protein synthesis; risk assessment
Abstract:
... Polycyclic Aromatic Hydrocarbons (PAHs) are persistent pollutants normally found in the environment as complex mixtures. Although several individual PAHs are classified as mutagenic and carcinogenic pollutants, the interaction effects between compounds in a mixture may trigger different toxicological mechanisms and, consequently, yield different effects to organisms which are not accounted for in ...
DOI:
10.1016/j.scitotenv.2022.158783

15. Investigation of the latency of Cyprinid herpesvirus 2 in apparently healthy farmed gibel carp, Carassius auratus gibelio

Author:
Chang Wei; Chaonan Xu; Yuyu Sun; Jinzhou Li; Motohiko Sano; Qiang Li
Source:
Aquaculture 2023 v.562 pp. 738854
ISSN:
0044-8486
Subject:
Carassius auratus; Carassius gibelio; Cyprinid herpesvirus 2; DNA; aquaculture; carrier state; gene expression; genes; goldfish; kidneys; necrosis; pathogens; transcription (genetics); viruses
Abstract:
... Cyprinid herpesvirus 2 (CyHV-2) is a highly contagious pathogen that causes herpesviral haematopoietic necrosis (HVHN) disease to goldfish, Carassius auratus and gibel carp, C. auratus gibelio. In this study, the virus persistence or latency in apparently healthy farmed gibel carp and reactivation in vitro were investigated. All of the freshly dissected kidney tissues of 42 individuals of asymptom ...
DOI:
10.1016/j.aquaculture.2022.738854

16. Label free flexible electrochemical DNA biosensor for selective detection of Shigella flexneri in real food samples

Author:
M.R. Ali; M.S. Bacchu; S. Das; S. Akter; M.M. Rahman; M.Aly Saad Aly; M.Z.H. Khan
Source:
Talanta 2023 v.253 pp. 123909
ISSN:
0039-9140
Subject:
DNA; Shigella flexneri; biosensors; detection limit; electrochemistry; electrodes; foodborne bacterial pathogens; indium tin oxide; melamine; sodium
Abstract:
... An effective tool for early-stage selective detection of the foodborne bacterial pathogen Shigella flexneri (S. flexneri) is essential for diagnosing infectious diseases and controlling outbreaks. Here, a label-free electrochemical DNA biosensor for monitoring S. flexneri is developed. To fabricate the biosensor, detection probe (capture probe) is immobilized on the surface of poly melamine (P-Mel ...
DOI:
10.1016/j.talanta.2022.123909

17. Methylome and transcriptome analysis of alters leaf phenotype with autotetraploid in grape

Author:
Xiaodong Xiang; Yukun Gao; Jianghui Cui; Genzeng Ren; Congpei Yin; Jinhua Chang
Source:
Scientia horticulturae 2023 v.307 pp. 111534
ISSN:
0304-4238
Subject:
5-methylcytosine; DNA; Vitis vinifera; anthocyanins; antioxidant activity; autotetraploidy; biosynthesis; bisulfites; cell walls; diploidy; epigenetics; gene expression; gene expression regulation; genomics; grapes; homeostasis; introns; leaf thickness; leaves; lipid metabolism; methylation; phenotype; photosynthesis; plant hormones; secondary metabolites; transcriptomics
Abstract:
... The doubling of the plant genome causes obvious morphological and physiological changes, and DNA 5-methylcytosine (5mCs) modification is a key epigenetic component that helps neopolyploids overcome challenges and acquire advantages. In the study of autotetraploid grape, we found that leaf thickness, photosynthetic and antioxidant capacity increased and anthocyanin coloring decreased in autotetrapl ...
DOI:
10.1016/j.scienta.2022.111534

18. Nano-gold mediated aptasensor for colorimetric monitoring of acrylamide: Smartphone readout strategy for on-site food control

Author:
Zahra Khoshbin; Marzieh Moeenfard; Khalil Abnous; Seyed Mohammad Taghdisi
Source:
Food chemistry 2023 v.399 pp. 133983
ISSN:
0308-8146
Subject:
DNA; acrylamides; aptasensors; breads; color; colorimetry; food chemistry; food safety; mobile telephones; nanogold
Abstract:
... A simple aptasensor is embedded in the internal surface of a micropipette tip as the aptasensor substrate for the label-free monitoring of acrylamide. The aptasensor is based on the formation of the triple-helix molecular switch (THMS) structure of the DNA strands that induces the salt-enforced aggregation of gold nanoparticles (AuNPs). A smartphone imaging readout-based strategy is applied to qua ...
DOI:
10.1016/j.foodchem.2022.133983

19. Nondestructive and rapid method for sex identification of the tropical sea cucumber Holothuria scabra by anal swab sampling

Author:
Zixuan E; Chuhang Cheng; Feifei Wu; Chunhua Ren; Rong Chen; Yingzhu Rao; Bo Ma; Xiao Jiang; Peng Luo; Xiaomin Li; Xin Zhang; Fajun Jiang; Chaoqun Hu; Ting Chen
Source:
Aquaculture 2023 v.562 pp. 738749
ISSN:
0044-8486
Subject:
DNA; Holothuria scabra; aquaculture; dimorphism; females; genomics; loop-mediated isothermal amplification; males; phenotype; rapid methods; reproduction; South East Asia
Abstract:
... As one of the most economically significant sea cucumber species, Holothuria scabra are naturally distributed in the Indo-West Pacific region and are widely cultural in Southeast Asia. The appearances of female and male H. scabra have no obvious phenotypic dimorphism. Thus, identification of sex-specific markers and establishment of sex detection methods are beneficial to artificially induced repr ...
DOI:
10.1016/j.aquaculture.2022.738749

20. Phenolics from noni (Morinda citrifolia L.) fruit alleviate obesity in high fat diet-fed mice via modulating the gut microbiota and mitigating intestinal damage

Author:
Ruimin Wang; Lu Wang; Shengwei Wang; Juntao Wang; Congyan Su; Lin Zhang; Congfa Li; Sixin Liu
Source:
Food chemistry 2023 v.402 pp. 134232
ISSN:
0308-8146
Subject:
Morinda citrifolia; anti-obesity effects; dysbiosis; food chemistry; fruits; high fat diet; inflammation; intestinal microorganisms; intestines; lipopolysaccharides; liver; obesity; occludins; oxidative stress; phenolic compounds; protein synthesis; short chain fatty acids
Abstract:
... Noni fruit has certain anti-obesity effect. However, the bioactive ingredients in noni fruit that contribute to anti-obesity activity as well as the relation between its anti-obesity activity and gut microbiota remain unclear. In this study, obese mice induced by high-fat diet (HFD) and were intervened with noni fruit phenolic extract (NFE) for 10 weeks. The results showed NFE supplementation decr ...
DOI:
10.1016/j.foodchem.2022.134232

21. Phylogeny, character evolution, and biogeography of the fern genus Bolbitis (Dryopteridaceae)

Author:
Li-Yun Nie; Liang Zhang; Zhen-Long Liang; Rossarin Pollawatn; Yue-Hong Yan; Ngan Thi Lu; Ralf Knapp; Xia Wan; Daniele Cicuzza; Xin-Xin Chen; Hong-Feng Chen; Ai-Hua Wang; Yu-Jie Liao; Fa-Guo Wang; Li-Bing Zhang
Source:
Molecular phylogenetics and evolution 2023 v.178 pp. 107633
ISSN:
1055-7903
Subject:
Bolbitis; DNA; biogeography; ferns and fern allies; geographical distribution; monophyly; paraphyly; polyphyly; Africa; Asia
Abstract:
... Bolbitis is a pantropical fern genus of Dryopteridaceae with ca. 80 species mainly in tropical Asia. Earlier studies confirmed the monophyly of Bolbitis when Mickelia is excluded and identified three major clades in Bolbitis. However, earlier studies are based on relatively small sampling and the majority of Asian species are not sampled. In this study, DNA sequences of three plastid markers of 16 ...
DOI:
10.1016/j.ympev.2022.107633

22. Real-time PCR, and Recombinase Polymerase Amplification combined with SYBR Green I for naked-eye detection, along with Propidium Monoazide (PMA) for the detection of viable patulin-producing fungi in apples and by-products

Author:
Foteini Roumani; Jorge Barros-Velázquez; Alejandro Garrido-Maestu; Marta Prado
Source:
Food control 2023 v.144 pp. 109347
ISSN:
0956-7135
Subject:
DNA; Penicillium; biochemical pathways; food safety; genes; patulin; propidium; quantitative polymerase chain reaction; recombinase polymerase amplification; toxicity
Abstract:
... Nowadays, the application of rapid molecular-based methods such as PCR/qPCR and isothermal techniques like Recombinase Polymerase Amplification (RPA), has increased in order to overcome some of the drawbacks of traditional culture-based methods due to their high sensitivity and specificity. One of the main limitations of these techniques is their inability to differentiate between live and dead mi ...
DOI:
10.1016/j.foodcont.2022.109347

23. Synergistic disinfection of aerosolized bacteria and bacteriophage by far-UVC (222-nm) and negative air ions

Author:
Y.H. Lu; H. Wu; H.H. Zhang; W.S. Li; A.C.K. Lai
Source:
Journal of hazardous materials 2023 v.441 pp. 129876
ISSN:
0304-3894
Subject:
DNA; Escherichia coli; Staphylococcus epidermidis; air; bacteriophages; bioaerosols; disinfection; ultraviolet radiation
Abstract:
... Air ionizers and 222-nm krypton-chlorine (KrCl) excilamp have proven to be effective disinfection apparatus for bacteria and viruses with limited health risks. We determined inactivation efficiencies by operating them individually and in combined modules. Gram-positive and gram-negative bacteria, non-enveloped dsDNA virus, and enveloped dsRNA virus were examined in a designed air disinfection syst ...
DOI:
10.1016/j.jhazmat.2022.129876

24. TMT-based quantitative proteomic and physiological analyses on lotus plumule of artificially aged seed in long-living sacred lotus Nelumbo nucifera

Author:
Dongli He; Mengmeng Cai; Meihui Liu; Pingfang Yang
Source:
Journal of proteomics 2023 v.270 pp. 104736
ISSN:
1874-3919
Subject:
DNA; Nelumbo nucifera; RNA; cell membranes; germination; histology; longevity; nutritive value; photosynthesis; physiological state; plumule; protein composition; proteome; proteomics; seed longevity; seed quality; starch; sucrose; vigor
Abstract:
... Seed longevity is important for the maintenance of seed nutritional quality, vigor, and germination potential during storage. Sacred lotus is known as one of the longest living seeds in the world and their ability to maintain longevity has been widely investigated. In this study, a suitable controlled deterioration treatment (CDT) method was first established to evaluate the vigor loss of lotus pl ...
DOI:
10.1016/j.jprot.2022.104736

25. Temporal profiling resolves the drivers of microbial nitrogen cycling variability in coastal sediments

Author:
Alexis J. Marshall; Lori Phillips; Andrew Longmore; Helen L. Hayden; Karla B. Heidelberg; Caixian Tang; Pauline Mele
Source:
Science of the total environment 2023 v.856 pp. 159057
ISSN:
0048-9697
Subject:
Archaea; DNA; RNA; ammonium; anaerobic ammonium oxidation; coastal zone management; detection limit; environment; genes; microbial activity; microbial nitrogen; nitrates; nitrites; nitrogen fixation; oxidation; phylogeny; seasonal variation; sediments; Australia
Abstract:
... Here we describe the potential for sediment microbial nitrogen-cycling gene (DNA) and activity (RNA) abundances to spatially resolve coastal areas impacted by seasonal variability in external nutrient inputs. Three sites were chosen within a nitrogen-limited embayment, Port Phillip Bay (PPB), Australia that reflect variability in both proximity to external nutrient inputs and the dominant form of ...
DOI:
10.1016/j.scitotenv.2022.159057

26. Understanding the influence of the bioaerosol source on the distribution of airborne bacteria in hospital indoor air

Author:
Víctor Pertegal; Engracia Lacasa; Pablo Cañizares; Manuel A. Rodrigo; Cristina Sáez
Source:
Environmental research 2023 v.216 pp. 114458
ISSN:
0013-9351
Subject:
DNA; Escherichia coli; World Health Organization; air; air flow; bioaerosols; cell membranes; hospitals; models; osmotic pressure; relative humidity; research; saliva; urine; wastewater
Abstract:
... The composition and concentration of airborne microorganisms in hospital indoor air has been reported to contain airborne bacteria and fungi concentrations ranged 10¹–10³ CFU/m³ in inpatients facilities which mostly exceed recommendations from the World Health Organization (WHO). In this work, a deeper knowledge of the performance of airborne microorganisms would allow improving the designs of the ...
DOI:
10.1016/j.envres.2022.114458

27. Unraveling the mechanism of the synergistic antimicrobial effect of cineole and carvacrol on Escherichia coli O157:H7 inhibition and its application on fresh-cut cucumbers

Author:
Keren Agyekumwaa Addo; Hui Li; Yigang Yu; Xinglong Xiao
Source:
Food control 2023 v.144 pp. 109339
ISSN:
0956-7135
Subject:
DNA; Escherichia coli O157; Shiga toxin; antimicrobial properties; bacteria; biofilm; carvacrol; cineole; exopolysaccharides; flagellum; food safety; fresh produce; genes; intimin; mechanism of action; quorum sensing; sanitizers; transcription (genetics); virulence
Abstract:
... The objective of this study was to evaluate the mechanism of the synergistic antimicrobial effect of cineole (CN) and carvacrol (CR) on E. coli and its application on fresh-cut cucumbers. Herein, the CN/CR synergy could effectively inhibit E. coli by destroying its bacterial cell structure and causing the leakage of intracellular organic matters. For the first time, CN/CR synergy interaction on E. ...
DOI:
10.1016/j.foodcont.2022.109339

28. An autonomous driven DNA walker-based electrochemical aptasensor for on-site detection of Ochratoxin A

Author:
Tongfu Huang; Mei Wang; Nian Hong; Hanfeng Cui; Qiqi Fan; Guobing Wei; Longshua Qin; Jing Zhang; Hao Fan
Source:
Talanta 2023 v.252 pp. 123785
ISSN:
0039-9140
Subject:
DNA; aptasensors; coordination polymers; electrochemistry; ochratoxin A
Abstract:
... Herein, an autonomous driven DNA walker-based aptasensor is proposed for the detection of Ochratoxin A (OTA). A nanoscale metal-organic framework (MOF) is synthesized and used to load Mn²⁺, a cofactor for DNAzyme. The DNA walker and Mn²⁺@MOF are assembled on the Au surface, integrating the DNA walker and metal auxiliary ions. The proposed sensor avoids the addition of metal auxiliary ions of DNAzy ...
DOI:
10.1016/j.talanta.2022.123785

29. A biodegradable and cofactor self-sufficient aptazyme nanoprobe for amplified imaging of low-abundance protein in living cells

Author:
Jiayao Xu; Lifang Yao; Xiaohong Zhong; Kun Hu; Shulin Zhao; Yong Huang
Source:
Talanta 2023 v.253 pp. 123983
ISSN:
0039-9140
Subject:
DNA; biodegradability; detection limit; flow cytometry; fluorescence; fluorescent dyes; nanorods; neoplasms; oligonucleotides
Abstract:
... Despite the progress on the analysis of proteins either in vitro or in vivo, detection and imaging of low-abundance proteins in living cells still remains challenging. Herein, a novel biodegradable and cofactor self-sufficient DNAzyme nanoprobe has been deve-loped for catalytic imaging of protein in living cells with signal amplification capacity. This DNAzyme nanoprobe is constructed by assemblin ...
DOI:
10.1016/j.talanta.2022.123983

30. An electrochemical biosensor for the highly sensitive detection of Staphylococcus aureus based on SRCA-CRISPR/Cas12a

Author:
Luqi Huang; Ning Yuan; Wei Guo; Yunzhe Zhang; Wei Zhang
Source:
Talanta 2023 v.252 pp. 123821
ISSN:
0039-9140
Subject:
DNA; Staphylococcus aureus; biosensors; electrodes; gold
Abstract:
... As one of the major foodborne pathogens, Staphylococcus aureus (S. aureus) can cause infectious diseases. In the current study, a novel electrochemical biosensor based on saltatory rolling circle amplification (SRCA) combined with CRISPR/Cas12a system was developed for the accurate detection of S. aureus. The thio-modified reporter probes (SH-ssDNA-MB) was immobilized on the surface of gold nanopa ...
DOI:
10.1016/j.talanta.2022.123821

31. A novel label-free universal biosensing platform based on CRISPR/Cas12a for biomarker detection

Author:
Xiaomei Mu; Xin Wang; Yuxin Qin; Yong Huang; Jianniao Tian; Shulin Zhao
Source:
Talanta 2023 v.251 pp. 123795
ISSN:
0039-9140
Subject:
DNA; biomarkers; biosensors; disease surveillance; enzyme activity; fluorescence; hydrolysis; microRNA; nanosilver
Abstract:
... The development of a biosensing platform with high sensitivity, high specificity, and low cost for the detection of biomarkers, especially one that is programmable and universal, is critical for disease surveillance and diagnosis, yet it remains a difficulty. Herein, we combined the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system with a fluorescent label-free biosenso ...
DOI:
10.1016/j.talanta.2022.123795

32. A novel method for detection of ochratoxin A in foods—Co-MOFs based dual signal ratiometric electrochemical aptamer sensor coupled with DNA walker

Author:
Yan Guan; Peng-Bin Si; Tong Yang; Yuan Wu; Yun-Hui Yang; Rong Hu
Source:
Food chemistry 2023 v.403 pp. 134316
ISSN:
0308-8146
Subject:
DNA; cobalt; detection limit; electrochemistry; electrodes; enzyme-linked immunosorbent assay; food chemistry; human health; kidneys; ochratoxin A; oligonucleotides; red wines; toluidine blue
Abstract:
... Ochratoxin A (OTA) is a naturally occurring mycotoxin that poses serious threats, such as kidney damage, to human health. Therefore, we developed a DNA walker-based dual-signal electrochemical ratiometric platform for OTA detection, which could overlook the variations in environmental and instrumental factors and DNA load densities. Cobalt metal–organic frameworks (Co-MOFs) and toluidine blue were ...
DOI:
10.1016/j.foodchem.2022.134316

33. The regulatory roles of DDIT4 in TDCIPP-induced autophagy and apoptosis in PC12 cells

Author:
Li Li; Lingyi Xi; Jin Wu; Zunquan Zhao; Youliang Chen; Weili Liu; Zhihui Pan; Mingzhu Liu; Danfeng Yang; Zhaoli Chen; Yanjun Fang
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 823-830
ISSN:
1001-0742
Subject:
DNA; Western blotting; apoptosis; autophagy; flame retardants; flow cytometry; humans; neurons; neurotoxicity; phosphates; phosphorylation; protective effect; rats; China
Abstract:
... Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is a commonly used organophosphate-based flame retardant and can bio-accumulate in human tissues and organs. As its structure is similar to that of neurotoxic organophosphate pesticides, the neurotoxicity of TDCIPP has raised widespread concerns. TDCIPP can increase neuronal apoptosis and induce autophagy. However, its regulatory mechanism remains un ...
DOI:
10.1016/j.jes.2022.02.046

34. 100 Years of Chromosome Research in Rye, Secale L.

Author:
Rolf Schlegel
Source:
Plants 2022 v.11 no.13 pp. -
ISSN:
2223-7747
Subject:
DNA; barley; cytogenetic analysis; fish; fluorescence; genes; grasses; haploidy; meiosis; microscopy; mitosis; reciprocal translocation; rice; rye; sequence analysis; tetraploidy; trisomics; wheat
Abstract:
... Although microscopy and genetics were still in their infancy, there are cytological results produced a hundred years ago that are still relevant today. Since the 1920s, rye has been a subject of chromosome research. It started by plotting its mitotic and meiotic chromosomes to determine genome size. After controversial evidence, it became clear that the base number is n = 7. However, structural di ...
DOI:
10.3390/plants11131753

35. 19th century family saga re-told by DNA recovered from postcard stamps

Author:
Cordula Haas; Christian Körner; Andrea Sulzer; Adelgunde Kratzer
Source:
Forensic science international 2022 v.330 pp. 111129
ISSN:
0379-0738
Subject:
DNA; children; forensic sciences; males; saliva
Abstract:
... Old postcards with stamps might help unravelling historical family stories and relationships. By employing ancient DNA recovered from world war I postage stamps, we disprove a family saga of an illegitimate child born in 1887. We developed a protocol to collect DNA from saliva, trapped and protected on the backside of postage stamps glued on postcards. With replicate STR analyses we were able to a ...
DOI:
10.1016/j.forsciint.2021.111129

36. 2,7-Diazapyrenes: a brief review on synthetic strategies and application opportunities

Author:
Anindita Mukherjee; Alexey A. Akulov; Sougata Santra; Mikhail V. Varaksin; Grigory A. Kim; Dmitry S. Kopchuk; Olga S. Taniya; Grigory V. Zyryanov; Oleg. N. Chupakhin
Source:
RSC advances 2022 v.12 no.15 pp. 9323-9341
ISSN:
2046-2069
Subject:
DNA; electronics; geometry; moieties
Abstract:
... 2,7-Diazapyrenes are promising azaaromatic scaffolds with a unique structural geometry and supramolecular properties. This core moiety and its derivatives with some N-methyl cations like N-methyl-2,7,-diazapyrenium, and N,N′-dimethyl-2,7-diazapyrenium attract special attention due to their challenging photophysical properties, especially in the context of interactions with DNA and some of its mono ...
DOI:
10.1039/d2ra00260d

37. 2-Oxopurine Riboside: A Dual Fluorescent Analog and Photosensitizer for RNA/DNA Research

Author:
Naishka E. Caldero-Rodríguez; Enrique M. Arpa; Diego J. Cárdenas; Lara Martínez-Fernández; Steffen Jockusch; Sourav Kanti Seth; Inés Corral; Carlos E. Crespo-Hernández
Source:
Journal of physical chemistry 2022 v.126 no.24 pp. 4483-4490
ISSN:
1520-5207
Subject:
DNA; RNA; absorption; aqueous solutions; fluorescence; nucleosides; photolysis; photosensitizing agents; quantum mechanics; singlet oxygen; solvents
Abstract:
... There is significant interest in developing suitable nucleoside analogs exhibiting high fluorescence and triplet yields to investigate the structure, dynamics, and binding properties of nucleic acids and promote selective photosensitized damage to DNA/RNA, respectively. In this study, steady-state, laser flash photolysis, time-resolved IR luminescence, and femtosecond broad-band transient absorpti ...
DOI:
10.1021/acs.jpcb.2c01113

38. 2D DNA lattices assembled from DX-coupled tiles

Author:
Wei Zhang; Chuan Jiang; Xin Guo; Mirza Muhammad Faran Ashraf Baig; Caihong Ni; Shou-Jun Xiao
Source:
Journal of colloid and interface science 2022 v.616 pp. 499-508
ISSN:
0021-9797
Subject:
DNA; atomic force microscopy; mechanics; mica; oligonucleotides
Abstract:
... Formation of coupled double crossovers (DXs) in a circular 106-mer oligonucleotide (c106nt) could generate stable tiles with the tile core span of 10 half turns. The large-span tiles with complicated curvatures and mechanics could assemble 2D lattices under different environments. Hence, 2D DNA lattice structures based on tile types and sequences, tile packing modes of corrugation and non-corrugat ...
DOI:
10.1016/j.jcis.2022.02.038

39. 2q35-rs13387042 variant and the risk of breast cancer: a case–control study

Author:
Abolfazl Nesaei; Zari Naderi Ghale-noie; Asma Khorshid Shamshiri; Fahimeh Afzaljavan; Mahdi Rivandi; Amir Tajbakhsh; Fatemeh Homaei Shandiz; Alireza Pasdar
Source:
Molecular biology reports 2022 v.49 no.5 pp. 3549-3557
ISSN:
0301-4851
Subject:
DNA; alleles; blood; breast neoplasms; breasts; carcinogenesis; case-control studies; demographic statistics; etiology; genetic background; genotyping; histopathology; lifestyle; molecular biology; risk; risk assessment; statistical analysis
Abstract:
... BACKGROUND: Breast Cancer is the most frequent neoplasm diagnosed among women worldwide. Genetic background and lifestyle/environment play a significant role in the disease etiology. According to Genome-wide association studies, some single-nucleotide polymorphisms such as 2q35-rs13387042–(G/A) have been introduced to be associated with breast cancer risk and features. In this study, we aimed to e ...
DOI:
10.1007/s11033-022-07195-6

40. A 5-color flow cytometry panel to assess plasma membrane integrity, acrosomal status, membrane lipid organization and mitochondrial activity of boar and stallion spermatozoa following liquid semen storage

Author:
Monike Quirino; Ulrike Jakop; Ana Paula Gonçalves Mellagi; Fernando Pandolfo Bortolozzo; Markus Jung; Martin Schulze
Source:
Animal reproduction science 2022 v.247 pp. 107076
ISSN:
0378-4320
Subject:
DNA; animal reproduction; boars; flow cytometry; fluorescent dyes; lipids; liquids; mitochondria; plasma membrane; propidium; rhodamines; semen; sperm quality; stallions
Abstract:
... For a more practically applicable analysis of different sperm characteristics, this study aimed to develop a 5-color flow cytometry (FC) panel to concurrently analyze four sperm parameters in liquid boar and stallion semen, using also a DNA-marker for selecting sperm cell events. From each of thirty extended boar semen doses and twelve stallion semen doses, six aliquots were taken. For evaluating ...
DOI:
10.1016/j.anireprosci.2022.107076

41. A 638‐gene phylogeny supports the recognition of twice as many species in the Malagasy endemic genus Capurodendron (Sapotaceae)

Author:
Carlos G. Boluda; Camille Christe; Yamama Naciri; Laurent Gautier
Source:
Taxon 2022 v.71 no.2 pp. 360-395
ISSN:
0040-0262
Subject:
DNA; Sapotaceae; conservation status; extinction; hardwood; new species; phylogeny; risk; statistical analysis; Madagascar
Abstract:
... The Malagasy genus Capurodendron currently accommodates 26 described species and is the largest genus of the family Sapotaceae in Madagascar. These species are frequently logged because of their valued hardwood, which potentially puts them at risk of extinction. Species‐level identifications are often problematic, and this hinders both an accurate assessment of their conservation status and the de ...
DOI:
10.1002/tax.12676

42. An 82 bp tandem repeat family typical of 3′ non-coding end of Gypsy/TAT LTR retrotransposons is conserved in Coffea spp. pericentromeres

Author:
Leonardo Adabo Cintra; Thaíssa Boldieri de Souza; Letícia Maria Parteka; Lucas Mesquita Barreto; Luiz Filipe Protasio Pereira; Marcos Letaif Gaeta; Romain Guyot; André Luís Laforga Vanzela
Source:
Genome 2022 v.65 no.3 pp. 137-151
ISSN:
1480-3321
Subject:
DNA; allotetraploidy; centromeres; chromatin; genome; microsatellite repeats; non-coding RNA; retrotransposons
Abstract:
... Coffea spp. chromosomes are very small and accumulate a variety of repetitive DNA families around the centromeres. However, the proximal regions of Coffea chromosomes remain poorly understood, especially regarding the nature and organisation of the sequences. Taking advantage of the genome sequences of C. arabica (2n = 44), C. canephora, and C. eugenioides (C. arabica progenitors with 2n = 22) and ...
DOI:
10.1139/gen-2021-0045

43. 90-day nose-only inhalation toxicity study of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in Sprague-Dawley rats

Author:
Shu-Chieh Hu; Seonggi Min; Hyun-Ki Kang; Dong-Jin Yang; Mallikarjuna Basavarajappa; Sherry M. Lewis; Kelly J. Davis; Ralph E. Patton; Matthew S. Bryant; Estatira Sepehr; Raul Trbojevich; Mason G. Pearce; Michelle E. Bishop; Wei Ding; Robert H. Heflich; MacKean P. Maisha; Robert Felton; Susan Chemerynski; Steven B. Yee; Melis Coraggio; Hans Rosenfeldt; R. Philip Yeager; Paul C. Howard; Yunan Tang
Source:
Food and chemical toxicology 2022 v.160 pp. 112780
ISSN:
0278-6915
Subject:
DNA; aerosols; air; biomarkers; blood; body weight; bone marrow; breathing; carcinogenesis; carcinogenicity; cigarettes; comet assay; food consumption; genotoxicity; histopathology; humans; inhalation toxicity; lowest observed effect level; lungs; metabolites; mutagens; nitrosamines; no observed adverse effect level; nose; propylene glycol; urine
Abstract:
... 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is one of the key tobacco-specific nitrosamines that plays an important role in human lung carcinogenesis. Repeated dose inhalation toxicity data on NNK, particularly relevant to cigarette smoking, however, is surprisingly limited. Hence, there is a lack of direct information available on the carcinogenic and potential non-carcinogenic effects o ...
DOI:
10.1016/j.fct.2021.112780

44. AAQSP increases mapping resolution of stable QTLs through applying NGS-BSA in multiple genetic backgrounds

Author:
Xiaoyu Wang; Xiaowei Zhang; Daoran Fan; Juwu Gong; Shaoqi Li; Yujie Gao; Aiying Liu; Linjie Liu; Xiaoying Deng; Yuzhen Shi; Haihong Shang; Yuanming Zhang; Youlu Yuan
Source:
Theoretical and applied genetics 2022 v.135 no.9 pp. 3223-3235
ISSN:
0040-5752
Subject:
DNA; Gossypium hirsutum; chromosome mapping; chromosomes; gene expression; genetic background; genetic improvement; heterosis; heterozygosity; lint cotton; nucleotide sequences; phenotypic variation
Abstract:
... KEY MESSAGE : In this study, we present AAQSP as an extension of existing NGS-BSA applications for identifying stable QTLs at high resolution. GhPAP16 and GhIQD14 fine mapped on chromosome D09 of upland cotton are identified as important candidate genes for lint percentage (LP). Bulked segregant analysis combined with next generation sequencing (NGS-BSA) allows rapid identification of genome seque ...
DOI:
10.1007/s00122-022-04181-1

45. AAV infection of bovine embryos: Novel, simple and effective tool for genome editing

Author:
Anna S. Krivonogova; Alexandra V. Bruter; Valeria A. Makutina; Yuliya D. Okulova; Leonid A. Ilchuk; Marina V. Kubekina; Alexandra Yu Khamatova; Tatiana V. Egorova; Vladimir S. Mymrin; Yuliya Yu Silaeva; Alexey V. Deykin; Maxim A. Filatov; Albina G. Isaeva
Source:
Theriogenology 2022 v.193 pp. 77-86
ISSN:
0093-691X
Subject:
DNA; animal reproduction; blastocyst; cattle; genetically modified organisms; genome; lectins; macrophages; serotypes
Abstract:
... Adeno-associated viruses (AAV) are widely used in the field of genetically modified organism production. In this work, transduction of bovine embryos by AAV was selected as a potential approach to perform genetic modifications: we have used recombinant AAV to produce GFP-positive bovine embryos. Five different AAV serotypes were used to evaluate their ability to deliver genetic material into the b ...
DOI:
10.1016/j.theriogenology.2022.09.007

46. AKT1 mediates multiple phosphorylation events that functionally promote HSF1 activation

Author:
Wen‐Cheng Lu; Ramsey Omari; Haimanti Ray; John Wang; Imade Williams; Curteisha Jacobs; Natasha Hockaden; Matthew L. Bochman; Richard L. Carpenter
Source:
FEBS journal 2022 v.289 no.13 pp. 3876-3893
ISSN:
1742-464X
Subject:
DNA; genes; heat shock proteins; heat shock response; heat stress; mass spectrometry; phosphorylation; phosphotransferases (kinases); proteome; transcription (genetics); transcription factors; transcriptional activation
Abstract:
... The heat stress response activates the transcription factor heat shock factor 1 (HSF1), which subsequently upregulates heat shock proteins to maintain the integrity of the proteome. HSF1 activation requires nuclear localization, trimerization, DNA binding, phosphorylation and gene transactivation. Phosphorylation at S326 is an important regulator of HSF1 transcriptional activity. Phosphorylation a ...
DOI:
10.1111/febs.16375

47. ATP Purinergic Receptor P2X1-Dependent Suicidal NETosis Induced by Cryptosporidium parvum under Physioxia Conditions

Author:
Seyed Sajjad Hasheminasab; Iván Conejeros; Zahady D. Velásquez; Tilman Borggrefe; Ulrich Gärtner; Faustin Kamena; Anja Taubert; Carlos Hermosilla
Source:
Biology 2022 v.11 no.3 pp. -
ISSN:
2079-7737
Subject:
Cryptosporidium parvum; DNA; Protozoa; acidification; cattle; cryptosporidiosis; elastase; extrusion; fluorescence microscopy; glucose; glycolysis; helminths; hyperoxia; intestines; lactic acid; neutrophils; oocysts; oxygen; oxygen consumption; plasma membrane; purinergic receptors; sporozoites; wildlife
Abstract:
... Cryptosporidiosis is a zoonotic intestinal disease that affects humans, wildlife, and neonatal cattle, caused by Cryptosporidium parvum. Neutrophil extracellular traps (NETs), also known as suicidal NETosis, are a powerful and ancient innate effector mechanism by which polymorphonuclear neutrophils (PMN) battle parasitic organisms like protozoa and helminths. Here, C. parvum oocysts and live sporo ...
DOI:
10.3390/biology11030442

48. ATP-Responsive Strand Displacement Coupling with DNA Origami/AuNPs Strategy for the Determination of Microcystin-LR Using Surface-Enhanced Raman Spectroscopy

Author:
Bingyang Huo; Ling Xia; Zhixian Gao; Gongke Li; Yuling Hu
Source:
Analytical chemistry 2022 v.94 no.34 pp. 11889-11897
ISSN:
1520-6882
Subject:
DNA; Raman spectroscopy; adenosine triphosphate; analytical chemistry; detection limit; liquid chromatography; microcystin-LR; nanomaterials; oligonucleotides; solid phase extraction; tandem mass spectrometry
Abstract:
... The DNA origami-mediated self-assembly strategy has emerged as a powerful tool in surface-enhanced Raman spectroscopy (SERS). However, these self-assembly approaches typically do not possess high detection specificity. Herein, a novel strategy based on adenosine triphosphate (ATP)-responsive strand displacement (ARSD) coupling with DNA origami/AuNPs for SERS analysis of microcystin-LR (MC-LR) is p ...
DOI:
10.1021/acs.analchem.2c02440

49. ATR-FTIR spectroscopy as a pre-screening technique for the PMI assessment and DNA preservation in human skeletal remains – A review

Author:
Leskovar Tamara; Zupanič Pajnič Irena; Jerman Ivan; Črešnar Matija
Source:
Quaternary international 2022 pp. -
ISSN:
1040-6182
Subject:
DNA; archaeology; chemometrics; collagen; forensic sciences; humans; postmortem changes; prediction; spectroscopy
Abstract:
... Skeletal remains from archaeological and forensic contexts are commonly used for various analyses, including DNA. Our research investigates relatively fast and inexpensive ATR-FTIR spectroscopy with further data manipulation as a potential pre-screening technique for assessing the preservation state of skeletal remains. We seek to correlate spectra and chemometric indices with the biological, chem ...
DOI:
10.1016/j.quaint.2022.09.004

50. AZD1222 (ChAdOx1 nCov-19): A Single-Dose biodistribution study in mice

Author:
Richard Stebbings; Gillian Armour; Vivian Pettis; Joanne Goodman
Source:
Vaccine 2022 v.40 no.2 pp. 192-195
ISSN:
0264-410X
Subject:
COVID-19 infection; DNA; blood; brain; intramuscular injection; nerve tissue; particulates; reticuloendothelial system; spinal cord; vaccines
Abstract:
... Biodistribution studies of adenovirus-based vaccines support their clinical development by evaluating their spread and persistence following in vivo administration. AZD1222 (ChAdox1 nCov-19) is a replication-deficient non-human adenovirus-vectored vaccine for coronavirus disease 2019. In this nonclinical study, the biodistribution of AZD1222 was assessed in mice for 29 days following intramuscular ...
DOI:
10.1016/j.vaccine.2021.11.028

51. Abortion and congenital transmission of Trypanosoma vivax in goats and ewes in semiarid northeastern Brazil

Author:
Jael Soares Batista; Wanderson Lucas Alves dos Santos; Ana Caroline Freitas Caetano de Sousa; Tiago da Silva Teófilo; Ana Carla Diógenes Suassuna Bezerra; Victor Hugo Vieira Rodrigues; José Ailton da Silva Filho; Tânia Vasconcelos Cavalcante; Kizzy Millenn de Freitas Mendonça Costa; Geysa Almeida Viana
Source:
Research in veterinary science 2022 v.149 pp. 125-127
ISSN:
0034-5288
Subject:
DNA; Trypanosoma vivax; blood; parasitemia; parasites; polymerase chain reaction; research; semiarid zones; trypanosomiasis; trypomastigotes; veterinary medicine; Brazil
Abstract:
... The main effects of trypanosomosis in Brazil are related to reproductive alterations. In this context, the present study aimed to report the occurrence of abortions in goats and sheeps in the semiarid region of Northeastern Brazil, associated with Trypanosoma vivax. Trypomastigotes forms visualized by Buffy coat technique (BCT) method in 68.7% of the goats and 50.0% of the ewes that aborted. PCR i ...
DOI:
10.1016/j.rvsc.2022.06.009

52. Absence of proteinase K‐resistant PrP in Korean Holstein cattle carrying potential bovine spongiform encephalopathy‐related E211K somatic mutation

Author:
Yong‐Chan Kim; Sae‐Young Won; Min‐Ju Jeong; Byung‐Hoon Jeong
Source:
Transboundary and emerging diseases 2022 v.69 no.2 pp. 805-812
ISSN:
1865-1674
Subject:
DNA; Holstein; alleles; bovine spongiform encephalopathy; cattle; females; germ cells; haplotypes; linkage disequilibrium; males; meat; medulla oblongata; monitoring; prions; proteinases; somatic mutation; Korean Peninsula
Abstract:
... Bovine spongiform encephalopathy (BSE) is a kind of prion disease caused by proteinase K‐resistant prion protein (PrPSᶜ) in cattle. Although BSE has been reported worldwide, BSE‐infected cases have never been reported in Korea. In a previous study, we identified BSE‐related somatic mutation E211K in 3 Korean Holstein cattle. In Korea, the BSE surveillance system has been established. However, seve ...
DOI:
10.1111/tbed.14053

53. Accelerated cycling PCR: A novel tool for rapid, sensitive and specific detection of single-nucleotide mutation within 30 min

Author:
Zhixian Luan; Yan Zhao; Yanling Wang; Cuiping Ma; Chao Shi
Source:
Journal of microbiological methods 2022 v.199 pp. 106527
ISSN:
0167-7012
Subject:
DNA; Helicobacter pylori; drug resistance; genotype; genotyping; mutation; rapid methods
Abstract:
... Rapid detection of single-nucleotide mutations (SNMs) has played a vital role for point-of-care testing. We herein first introduced accelerated thermal cycling into conventional allele-specific qPCR (AS-qPCR), named accelerated cycling PCR (AC-PCR) to achieve rapid and sensitive detection of SNM. It could simultaneously detect 10 copies of H. pylori DNA and identify its clarithromycin-resistance g ...
DOI:
10.1016/j.mimet.2022.106527

54. Accumulation of polystyrene microplastics induces liver fibrosis by activating cGAS/STING pathway

Author:
Rong Shen; Keren Yang; Xin Cheng; Changlei Guo; Xiqing Xing; Haonan Sun; Disheng Liu; Xiangwen Liu; Degui Wang
Source:
Environmental pollution 2022 v.300 pp. 118986
ISSN:
0269-7491
Subject:
DNA; DNA damage; cytokines; fibronectins; fibrosis; hepatocytes; liver; liver cirrhosis; microplastics; mitochondria; pollution; polystyrenes; risk; toxicity
Abstract:
... The environmental pollution from microplastics has caused concern from the whole society due to its harm to organisms. However, the effect of microplastics on liver damage and fibrosis remains unclear in the case of long-term accumulation. The present study demonstrated that the 0.1 μm microplastic could enter hepatocytes from circulation and result liver damage even at a low concentration. Microp ...
DOI:
10.1016/j.envpol.2022.118986

55. Achieving maximum efficiency of Mungbean yellow mosaic India virus infection in mungbean by agroinoculation

Author:
Palaiyur N. Sivalingam; Narasimham Dokka; Mahesh M. Mahajan; Bhimeshwari Sahu; Ashish Marathe; Pankaj Kaushal; Probir Kumar Ghosh
Source:
3 Biotech 2022 v.12 no.1 pp. 29
ISSN:
2190-572X
Subject:
Agrobacterium; Aleyrodidae; DNA; Mungbean yellow mosaic India virus; Mungbean yellow mosaic virus; acetosyringone; genomics; germplasm; leaves; meristems; mung beans; pathogenicity; soil
Abstract:
... Mungbean is one of the important food legumes in the Indian–sub-continent. Yellow mosaic disease, caused by Mungbean yellow mosaic virus and Mungbean yellow mosaic India virus (MYMIV) poses a severe threat to its production. Agroinoculation has been the most preferred way to test the function of genomic components of these viruses. However, the available inoculation methods are not as efficient as ...
DOI:
10.1007/s13205-021-03088-w

56. Achieving robust somatic mutation detection with deep learning models derived from reference data sets of a cancer sample

Author:
Sayed Mohammad Ebrahim Sahraeian; Li Tai Fang; Konstantinos Karagiannis; Malcolm Moos; Sean Smith; Luis Santana-Quintero; Chunlin Xiao; Michael Colgan; Huixiao Hong; Marghoob Mohiyuddin; Wenming Xiao
Source:
Genome biology 2022 v.23 no.1 pp. 12
ISSN:
1474-760X
Subject:
DNA; DNA damage; cell lines; computer simulation; gene frequency; genome; genomics; models; neoplasm cells; neoplasms; somatic mutation
Abstract:
... BACKGROUND: Accurate detection of somatic mutations is challenging but critical in understanding cancer formation, progression, and treatment. We recently proposed NeuSomatic, the first deep convolutional neural network-based somatic mutation detection approach, and demonstrated performance advantages on in silico data. RESULTS: In this study, we use the first comprehensive and well-characterized ...
DOI:
10.1186/s13059-021-02592-9

57. Acid adaptive response of Alicyclobacillus acidoterrestris: A strategy to survive lethal heat and acid stresses

Author:
Ning Zhao; Junnan Xu; Lingxia Jiao; Miaomiao Liu; Tong Zhang; Jun Li; Xinyuan Wei; Mingtao Fan
Source:
Food research international 2022 v.157 pp. 111364
ISSN:
0963-9969
Subject:
Alicyclobacillus acidoterrestris; DNA; DNA repair; Fourier transform infrared spectroscopy; acid tolerance; biosynthesis; cell membranes; decarboxylation; fatty acids; food research; fruit juices; gene expression; gene expression regulation; glutamic acid; heat; homeostasis; homologous recombination; juice industry; pH; pasteurization; phospholipids; roughness; shrinkage; spoilage; sporulation; transcriptomics; urease; zwitterions
Abstract:
... Alicyclobacillus acidoterrestris causes the spoilage of pasteurized acidic fruit juice, seriouslydecreasing quality and posing a significant safety concern. We previously discovered that acid adaptation could induce stress adaptive responses of A. acidoterrestris, however, the underlying mechanisms of this induction have not been fully elucidated. In this work, the effects of acid adaptation (pH = ...
DOI:
10.1016/j.foodres.2022.111364

58. Aconitine induces autophagy via activating oxidative DNA damage-mediated AMPK/ULK1 signaling pathway in H9c2 cells

Author:
Wenlin Wang; Jialuo Jiang; Yan Huang; Fu Peng; Tingting Hu; Jiayang Wu; Xiaoqi Pan; Chaolong Rao
Source:
Journal of ethnopharmacology 2022 v.282 pp. 114631
ISSN:
0378-8741
Subject:
Aconitum; DNA; DNA damage; aconitine; antioxidants; arrhythmia; autophagosomes; autophagy; cardiomyocytes; cardiotoxicity; cell viability; confocal microscopy; death; enzyme-linked immunosorbent assay; fibrosis; flow cytometry; phosphorylation; protein synthesis; rheumatoid arthritis; toxicity; traditional medicine
Abstract:
... Aconitum species, with a medicinal history of 2000 years, was traditionally used in the treatment of rheumatism, arthritis, bruises, and pains. However, many studies have reported that Aconitum species can cause arrhythmia in experimental animals, resulting in myocardial fibrosis and cardiomyocyte damage. Cardiotoxicity is the main toxic effect of aconitine, but the detailed mechanism remains uncl ...
DOI:
10.1016/j.jep.2021.114631

59. Actin Contributes to the Hyperexpression of Baculovirus Polyhedrin (polh) and p10 as a Component of Transcription Initiation Complex (TIC)

Author:
Nan Chen; Guanping Chen; Xiangshuo Kong; Xiaofeng Wu
Source:
Viruses 2022 v.14 no.1 pp. -
ISSN:
1999-4915
Subject:
Baculoviridae; DNA; DNA-directed RNA polymerase; actin; chromatin immunoprecipitation; genetic vectors; luciferase; physiological transport; transcription initiation
Abstract:
... Hyperexpression of polh and p10, two very late genes, is one of the remarkable characteristics in the baculovirus life cycle. However, the mechanisms underlying the hyperexpression of these two genes are still incompletely understood. In this study, actin was identified as a highly potential binding partner of polh and p10 promoters by conducting DNA pull-down and LC–MS/MS analyses. Inhibiting act ...
DOI:
10.3390/v14010153

60. Action mode of cuminaldehyde against Staphylococcus aureus and its application in sauced beef

Author:
Hui Li; Mao Zhang; Keren Agyekumwaa Addo; Yigang Yu; Xinglong Xiao
Source:
Lebensmittel-Wissenschaft + Technologie 2022 v.155 pp. 112924
ISSN:
0023-6438
Subject:
DNA; Staphylococcus aureus; antibacterial properties; beef; cell membranes; food industry; gel electrophoresis; odors; permeability; spectral analysis; taste
Abstract:
... The antibacterial mechanisms of cuminaldehyde (p-Isopropylbenzaldehyde, CUM) against Staphylococcus aureus ATCC 6538 (S. aureus) were investigated from the aspects of membrane damage, and interaction with genomic DNA. The results demonstrated that the MIC of CUM against S. aureus was 0.8 μL/mL. Approximately 6 log CFU/mL of S. aureus could be killed after treatment with MIC of CUM after 8 h. Addit ...
DOI:
10.1016/j.lwt.2021.112924

61. Activation of Henneguya ictaluri Actinospores by Nonictalurid Fish Species, with Implications for Management of Proliferative Gill Disease in Catfish Aquaculture

Author:
Bradley M. Richardson; Charles C. Mischke; T. Graham Rosser; Ethan T. Woodyard; Cynthia Ware; David J. Wise; Matt J. Griffin
Source:
North American journal of aquaculture 2022 v.84 no.3 pp. 313-324
ISSN:
1522-2055
Subject:
DNA; Gambusia affinis; Henneguya; Ictalurus punctatus; biomass; catfish; death; host specificity; inflammation; morbidity; mucus; parasites; parasitoses
Abstract:
... Proliferative gill disease (PGD), caused by the myxozoan Henneguya ictaluri, is an important parasitic disease in U.S. catfish aquaculture. Continuous exposure of Channel Catfish Ictalurus punctatus to the actinospore stage of H. ictaluri results in a severe inflammatory response at the gills, leading to morbidity and death. Previous work indicates that the chemical cues in fish mucus that are rec ...
Handle:
10113/7709618
DOI:
10.1002/naaq.10242

62. Active equine parvovirus‐hepatitis infection is most frequently detected in Austrian horses of advanced age

Author:
Marcha Badenhorst; Phebe de Heus; Angelika Auer; Birthe Tegtmeyer; Alexander Stang; Katharina Dimmel; Alexander Tichy; Jakub Kubacki; Claudia Bachofen; Eike Steinmann; Jessika M. V. Cavalleri
Source:
Equine veterinary journal 2022 v.54 no.2 pp. 379-389
ISSN:
0425-1644
Subject:
DNA; Equidae; Hepacivirus; albumins; bile; cross-sectional studies; elderly; gamma-glutamyltransferase; genetic variation; geographical distribution; glutamate dehydrogenase; hepatitis; horses; luciferase; mixed infection; pathogenesis; phylogeny; polymerase chain reaction; precipitin tests; seroprevalence; Austria
Abstract:
... BACKGROUND: Equine parvovirus‐hepatitis (EqPV‐H) research is in its infancy. Information regarding prevalence, geographical distribution, genetic diversity, pathogenesis and risk factors enhances understanding of this potentially fatal infection. OBJECTIVES: Determining the prevalence of EqPV‐H in Austrian equids. Investigating factors increasing probability of infection, liver‐associated biochemi ...
DOI:
10.1111/evj.13444

63. Active meiosis during dinoflagellate blooms: A ‘sex for proliferation’ hypothesis

Author:
Senjie Lin; Liying Yu; Xiaomei Wu; Meizhen Li; Yaqun Zhang; Hao Luo; Hongfei Li; Tangcheng Li; Ling Li
Source:
Harmful algae 2022 v.118 pp. 102307
ISSN:
1568-9883
Subject:
DNA; Karenia mikimotoi; Prorocentrum; cell proliferation; encystment; flow cytometry; genetic variation; germination; meiosis; sexual reproduction
Abstract:
... In dinoflagellates, sexual reproduction is best known to be induced by adverse environmental conditions and culminate in encystment for survival (‘sex for encystment’). Although increasing laboratory observations indicate that sex can lead to production of vegetative cells bypassing encystment, the occurrence of this alternative pathway in natural populations and its ecological roles remain poorly ...
DOI:
10.1016/j.hal.2022.102307

64. Acute Exposure to Crude Oil Induces Epigenetic, Transcriptional and Metabolic Changes in Juvenile Sciaenops ocellatus

Author:
Mayra A. Cañizares-Martínez; Mercedes Quintanilla-Mena; Marcela Del-Río-García; Isajav Rivas-Reyes; María V. Patiño-Suárez; Victor M. Vidal-Martínez; M. Leopoldina Aguirre-Macedo; Carlos A. Puch-Hau
Source:
Bulletin of environmental contamination and toxicology 2022 v.108 no.1 pp. 85-92
ISSN:
0007-4861
Subject:
DNA; DNA methylation; Sciaenops ocellatus; acute exposure; aldehyde dehydrogenase; bile; bioassays; biotransformation; catalase; cytochrome P-450; epigenetics; fish; glutathione; glutathione peroxidase; juveniles; metabolites; oils; oxidative stress; petroleum; pollution; polycyclic aromatic hydrocarbons; reproductive system; toxicology; transcription (genetics); vitellogenin; xenobiotics
Abstract:
... In this study, we report molecular and metabolic responses of Sciaenops ocellatus during an acute oil exposure bioassay (100, 800 and 8000 mg 1–¹ of crude oil). The global DNA methylation and expression profiles of key genes of the xenobiotic biotransformation system (cytochrome P450 1A [cyp1a] and glutathione S-tranferase [gst]), oxidative stress system (glutathione peroxidase [gpx], catalase [ca ...
DOI:
10.1007/s00128-021-03241-4

65. Acute Oral Administration of Cerium Oxide Nanoparticles Suppresses Lead Acetate–Induced Genotoxicity, Inflammation, and ROS Generation in Mice Renal and Cardiac Tissues

Author:
Hanan Ramadan Hamad Mohamed
Source:
Biological trace element research 2022 v.200 no.7 pp. 3284-3293
ISSN:
0163-4984
Subject:
DNA; DNA damage; diphenylamine; genotoxicity; heart; inflammation; interleukin-6; kidneys; lead; lead acetate; males; nanoceria; oral administration; pollutants; prostaglandin synthase; reactive oxygen species; research; trace elements
Abstract:
... Lead, a highly toxic pollutant, causes numerous health problems and affects nearly all biological systems thus arousing interest in using antioxidants to reduce its toxic effects. Therefore, the undertaken study estimated the influence of cerium oxide nanoparticles (CeO₂-NPs) on the lead acetate–induced genotoxicity and inflammation in the kidney and heart tissues of mice. Twenty male mice were ra ...
DOI:
10.1007/s12011-021-02914-9

66. Adaptation of Phytophthora nicotianae to Multiple Sources of Partial Resistance in Tobacco

Author:
Jing Jin; Kestrel L. McCorkle; Vicki Cornish; Ignazio Carbone; Ramsey S. Lewis; H. David Shew
Source:
Plant disease 2022 v.106 no.3 pp. 906-917
ISSN:
0191-2917
Subject:
DNA; Nicotiana rustica; Phytophthora nicotianae; black shank disease; disease control; disease severity; genotype; greenhouses; quantitative traits; resistance genes; root rot; tobacco
Abstract:
... Host resistance is an important tool in the management of black shank disease of tobacco. Race development leads to rapid loss of single-gene resistance, but the adaptation by Phytophthora nicotianae to sources of partial resistance from Beinhart 1000, Florida 301, and the Wz gene region introgressed from Nicotiana rustica is poorly characterized. In greenhouse environments, host genotypes with qu ...
DOI:
10.1094/PDIS-06-21-1241-RE

67. Adaptive genetic diversity of dominant species contributes to species co-existence and community assembly

Author:
Qiao-Ming Li; Chao-Nan Cai; Wu-Mei Xu; Min Cao; Li-Qing Sha; Lu-Xiang Lin; Tian-Hua He
Source:
Plant diversity 2022 v.44 no.3 pp. 271-278
ISSN:
2468-2659
Subject:
DNA; Parashorea; community structure; dominant species; ecological competition; equations; evolution; genetic variation; models; nitrogen; rain forests; soil; species diversity; trees; understory
Abstract:
... The synthesis of evolutionary biology and community ecology aims to understand how genetic variation within one species can shape community properties and how the ecological properties of a community can drive the evolution of a species. A rarely explored aspect is whether the interaction of genetic variation and community properties depends on the species' ecological role. Here we investigated th ...
DOI:
10.1016/j.pld.2021.11.002

68. Addressing nontarget amplification in DNA metabarcoding studies of arthropod-feeding rodents

Author:
Dylan M. Klure; Robert Greenhalgh; M. Denise Dearing
Source:
Mammal research 2022 v.67 no.4 pp. 499-509
ISSN:
2199-2401
Subject:
DNA; DNA barcoding; Neotoma; arthropods; cytochrome-c oxidase; databases; diet; excreta; loci; mitochondria; relative risk; research; rodents
Abstract:
... High-throughput sequencing approaches have revolutionized how we study animal diets by enabling the detection of dietary components from the metabarcoding of DNA in excrement. Mitochondrial cytochrome oxidase C subunit I (mtCOI) DNA metabarcoding is commonly used to study the diets of arthropod-feeding animals; however, this approach is susceptible to nontarget amplification of the consumer specie ...
DOI:
10.1007/s13364-022-00646-2

69. Adenine base-editing-mediated exon skipping induces gene knockout in cultured pig cells

Author:
Xiang-xing Zhu; Jia-sheng Pan; Tao Lin; Ye-cheng Yang; Qiu-yan Huang; Shuai-peng Yang; Zi-xiao Qu; Zi-sheng Lin; Jian-cong Wen; Ai-fen Yan; Juan Feng; Lian Liu; Xiao-li Zhang; Jia-hong Lu; Dong-sheng Tang
Source:
Biotechnology letters 2022 v.44 no.1 pp. 59-76
ISSN:
0141-5492
Subject:
CRISPR-Cas systems; DNA; adenine; biosafety; biotechnology; eukaryotic cells; exons; gene expression; gene targeting; introns; medicine; protein depletion; somatic cells; spliceosomes; swine; transfection
Abstract:
... Gene-knockout pigs have important applications in agriculture and medicine. Compared with CRISPR/Cas9, Adenine base editor (ABE) convert single A·T pairs to G·C pairs in the genome without generating DNA double-strand breaks, and this method has higher accuracy and biosafety in pig genetic modification. However, the application of ABE in pig gene knockout is limited by protospacer-adjacent motif s ...
DOI:
10.1007/s10529-021-03214-x

70. Adenine imprinted beads as a novel selective extracellular DNA extraction method reveals underestimated prevalence of extracellular antibiotic resistance genes in various environments

Author:
Qingbin Yuan; Yi Wang; Shangjie Wang; Ruiqing Li; Junlu Ma; Yijing Wang; Ruonan Sun; Yi Luo
Source:
Science of the total environment 2022 v.852 pp. 158570
ISSN:
0048-9697
Subject:
DNA; adenine; air; antibiotic resistance; environment; public health; rivers; vegetables
Abstract:
... Despite severe threats of extracellular antibiotic resistance genes (eARGs) towards public health in various environments, advanced studies have been hindered mainly by ineffective extracellular DNA (exDNA) extraction methods, which is challenged by trace levels of exDNA and inference from abundant coexisting compounds. This study developed a highly selective exDNA extraction method based on molec ...
DOI:
10.1016/j.scitotenv.2022.158570

71. Administration of the probiotic Lactiplantibacillus paraplantarum is effective in controlling hyperphosphatemia in 5/6 nephrectomy rat model

Author:
Sung Jin Moon; Junsang Hwang; Woo Kyung Kang; Jeong-Pin Ahn; Hyo Jin Kim
Source:
Life sciences 2022 v.306 pp. 120856
ISSN:
0024-3205
Subject:
DNA; absorption; animal models; blood serum; fermentation; humans; immunomodulation; indoles; intestines; kidney diseases; lactic acid; multigene family; phosphates; phosphorus; probiotics; sulfates
Abstract:
... Several studies have linked gut microbes to human diseases. Most of the mechanisms by which lactic acid bacteria have beneficial effects on the human body are related to immune modulation. Controlled studies of the ability of lactic acid bacteria to absorb phosphorus directly from the intestine and thereby control serum phosphorus level in in vivo uremic animal models are limited. We screened lact ...
DOI:
10.1016/j.lfs.2022.120856

72. Advanced wastewater treatment with ozonation and granular activated carbon filtration: Inactivation of antibiotic resistance targets in a long-term pilot study

Author:
K. Slipko; D. Reif; H. Schaar; E. Saracevic; A. Klinger; L. Wallmann; J. Krampe; M. Woegerbauer; P. Hufnagl; N. Kreuzinger
Source:
Journal of hazardous materials 2022 v.438 pp. 129396
ISSN:
0304-3894
Subject:
DNA; Escherichia coli; absorption; activated carbon; activated sludge; advanced wastewater treatment; ampicillin; antibiotic resistance; filtration; nitrites; ozonation; ozone; sulfamethoxazole; trimethoprim
Abstract:
... The inactivation of antibiotic resistant bacteria (ARB) and genes (ARGs) in an advanced plant combining ozonation and granular activated carbon (GAC) filtration applied for effluent after conventional activated sludge treatment at a full-scale urban wastewater treatment plant was investigated for over 13 consecutive months. The nitrite compensated specific ozone dose ranged between 0.4 and 0.7 g O ...
DOI:
10.1016/j.jhazmat.2022.129396

73. Advances in Nematode Identification: A Journey from Fundamentals to Evolutionary Aspects

Author:
Kaisar Ahmad Bhat; Rakeeb Ahmad Mir; Asmat Farooq; Madhiya Manzoor; Ammarah Hami; Kaisar Ahmad Allie; Shaheen Majeed Wani; M. N. Khan; R. Z. Sayyed; Peter Poczai; Waleed Hassan Almalki; Sajad Majeed Zargar; Ali Asghar Shah
Source:
Diversity 2022 v.14 no.7 pp. -
ISSN:
1424-2818
Subject:
DNA; Nematoda; amino acids; morphometry; phylogeny; sibling species; social welfare; taxonomy
Abstract:
... Nematodes are non-segmented roundworms evenly distributed with various habitats ranging to approximately every ecological extremity. These are the least studied organisms despite being the most diversified group. Nematodes are the most critical equilibrium-maintaining factors, having implications on the yield and health of plants as well as well-being of animals. However, taxonomic knowledge about ...
DOI:
10.3390/d14070536

74. Advances in protoplast transfection promote efficient CRISPR/Cas9-mediated genome editing in tetraploid potato

Author:
Gulzar A. Rather; Dana Ayzenshtat; Paula Teper-Bamnolker; Manoj Kumar; Zohar Forotan; Dani Eshel; Samuel Bocobza
Source:
Planta 2022 v.256 no.1 pp. 14
ISSN:
0032-0935
Subject:
CRISPR-Cas systems; DNA; callus; gene editing; genes; kanamycin; neomycin; potatoes; protoplasts; tetraploidy; transcription factors; transfection
Abstract:
... MAIN CONCLUSION: An efficient method of DNA-free gene-editing in potato protoplasts was developed using linearized DNA fragments, UBIQUITIN10 promoters of several plant species, kanamycin selection, and transient overexpression of the BABYBOOM transcription factor. Plant protoplasts represent a reliable experimental system for the genetic manipulation of desired traits using gene editing. Neverthe ...
DOI:
10.1007/s00425-022-03933-z

75. Advances in the exact nuclear Overhauser effect 2018–2022

Author:
Alya Hussain; Natasia Paukovich; Morkos A. Henen; Beat Vögeli
Source:
Methods 2022 v.206 pp. 87-98
ISSN:
1046-2023
Subject:
DNA; RNA; nuclear magnetic resonance spectroscopy
Abstract:
... The introduction of the exact nuclear Overhauser enhancement (eNOE) methodology to solution-state nuclear magnetic resonance (NMR) spectroscopy results in tighter distance restraints from NOEs than in convention analysis. These improved restraints allow for higher resolution in structure calculation and even the disentanglement of different conformations of macromolecules. While initial work prima ...
DOI:
10.1016/j.ymeth.2022.08.006

76. Advances in the quality control of fenugreek seeds using chromatographic, spectroscopic and DNA‐based techniques: A comprehensive review

Author:
Nariman H. Kandil; Iriny M. Ayoub; Sherweit H. El‐Ahmady; Safaa A. El‐Moghazy
Source:
Phytochemical analysis 2022 v.33 no.2 pp. 155-169
ISSN:
0958-0344
Subject:
DNA; antioxidants; chemometrics; fenugreek; gas chromatography; high performance liquid chromatography; nuclear magnetic resonance spectroscopy; phytochemicals; quality control; saponins; thin layer chromatography
Abstract:
... INTRODUCTION: Fenugreek has been used in traditional remedies since ancient times. It has a long history of use against medical ailments as an antidiabetic, anticarcinogenic, hypocholesterolemic, antioxidant, antibacterial, hypoglycemic, gastric stimulant, and anti‐anorexia agent. The major active constituents include alkaloids, fibres, saponins, proteins, and amino acids. OBJECTIVES: To provide a ...
DOI:
10.1002/pca.3091

77. Aerobic naphthenic acid-degrading bacteria in petroleum-coke improve oil sands process water remediation in biofilters: DNA-stable isotope probing reveals methylotrophy in Schmutzdecke

Author:
Muhammad Arslan; Jochen A. Müller; Mohamed Gamal El-Din
Source:
Science of the total environment 2022 v.815 pp. 151961
ISSN:
0048-9697
Subject:
DNA; Methylobacillus; Pseudomonas; Pseudoxanthomonas; aeration; anaerobic digestion; bioaugmentation; biofilters; biofiltration; carbon; environment; fluorescent dyes; methane; methane production; oils; petroleum
Abstract:
... There is an increasing interest in treatment of oil sands process water (OSPW) via biofiltration with petroleum coke (PC) as a substratum. In fixed bed biofilters (FBBs) with PC, the dominance of anaerobic digestion of dissolved organics results in poor removal of naphthenic acids (NAs) along with a high degree of methanogenesis. In this study, the operation of FBBs was modified to improve OSPW re ...
DOI:
10.1016/j.scitotenv.2021.151961

78. Aeromonas veronii infection remarkably increases expression of lysozymes in grass carp (Ctenopharyngodon idellus) and injection of lysozyme expression cassette along with QCDC adjuvant significantly upregulates immune factors and decreases cumulative mortality

Author:
Pei Chen; Duo Jin; Shijia Yang; Xiaojing Yu; Ganfeng Yi; Shengbiao Hu; Yunjun Sun; Yibo Hu; Jun Cui; Jie Rang; Liqiu Xia
Source:
Microbial pathogenesis 2022 v.169 pp. 105646
ISSN:
0882-4010
Subject:
Aeromonas veronii; Ctenopharyngodon idella; DNA; Escherichia coli; adjuvants; alkaline phosphatase; antibacterial properties; bacteria; blood serum; cholesterol; immunostimulants; interleukin-8; kidneys; liver; lysozyme; pathogenesis; spleen; survival rate; toxicity
Abstract:
... Aeromonas veronii AvX005 is a pathogenic bacterium with high toxicity to grass carp (Ctenopharyngodon idellus). The expression levels of g-type (goose-type lysozyme, Lys-g) and c-type lysozyme (chicken-type lysozyme, Lys-c) in the spleen of grass carp infected with AvX005 were significantly increased by approximately 4.5 times and 27 times, respectively. The recombinant proteins rLys-g and rLys-c ...
DOI:
10.1016/j.micpath.2022.105646

79. African or Asiatic origin? Genome analyses solved the mystery of the hybrid origin of the rescued lion cub

Author:
Abhishek Singh; Mukesh Thakur; Lalit Kumar Sharma; Dhriti Banerjee
Source:
Gene 2022 v.817 pp. 146228
ISSN:
0378-1119
Subject:
DNA; Panthera leo leo; Y chromosome; genes; hybrids; law enforcement; mitochondria; mitochondrial genome; paternity; phylogeny; wildlife
Abstract:
... Species assignment of any seized material using DNA analysis has been a routine and widely accepted standard procedure in providing scientific advisory for the legal prosecution of wildlife cases. Scientific advancements and rigorous application of genetic tools have led to the development of a variety of molecular markers with their defined efficacy in wildlife forensics. However, in a few unusua ...
DOI:
10.1016/j.gene.2022.146228

80. African swine fever virus M1249L protein antagonizes type I interferon production via suppressing phosphorylation of TBK1 and degrading IRF3

Author:
Shuai Cui; Yang Wang; Xintao Gao; Ting Xin; Xixi Wang; Hainan Yu; Shiyu Chen; Yajun Jiang; Qing Chen; Fei Jiang; Dongyue Wang; Xiaoyu Guo; Hong Jia; Hongfei Zhu
Source:
Virus research 2022 v.319 pp. 198872
ISSN:
0168-1702
Subject:
African swine fever virus; DNA; dose response; immune evasion; innate immunity; interferon regulatory factor-3; phosphorylation; research; viruses
Abstract:
... Cyclic GMP-AMP synthase (cGAS) is a major DNA sensor. The recognition of cytosolic DNA by cGAS triggers a robust innate immune response that restricts the replication of diverse viral pathogens through the type I interferon (IFN) and nuclear factor-κB (NF-κB) pathways. African swine fever virus (ASFV) is a large and complex DNA virus reported to strongly inhibit the cGAS-STING signaling pathway. H ...
DOI:
10.1016/j.virusres.2022.198872

81. African swine fever virus pE301R negatively regulates cGAS-STING signaling pathway by inhibiting the nuclear translocation of IRF3

Author:
Xiaohong Liu; Hongyang Liu; Guangqiang Ye; Mengdi Xue; Huibin Yu; Chunying Feng; Qiongqiong Zhou; Xuemin Liu; Longfeng Zhang; Shuang Jiao; Changjiang Weng; Li Huang
Source:
Veterinary microbiology 2022 v.274 pp. 109556
ISSN:
0378-1135
Subject:
African swine fever; African swine fever virus; DNA; amino acids; dose response; innate immunity; microbiology; mortality; phosphorylation
Abstract:
... African swine fever (ASF) is a highly contagious and lethal infectious disease of domestic pigs and wild boars by the African swine fever virus (ASFV). ASFV infects domestic pigs with the mortality rate approaching 100 % at acute stage of infection. The cGAS-STING-mediated antiviral responses are wildly accepted that cGAS acts as DNA sensor for sensing of viral DNA during DNA virus infection. Howe ...
DOI:
10.1016/j.vetmic.2022.109556

82. Ag@Pyc Nanocapsules as Electrochemiluminescence Emitters for an Ultrasensitive Assay of the APE1 Activity

Author:
Duo-Duo Chai; Ying Zhuo; Ting-Ting Tu; Hong-Ling Li; Ruo Yuan; Sha-Ping Wei
Source:
Analytical chemistry 2022 v.94 no.27 pp. 9934-9939
ISSN:
1520-6882
Subject:
DNA; analytical chemistry; biosensors; detection limit; early diagnosis; electrochemiluminescence; humans; nanocapsules; nanosilver; sulfates
Abstract:
... Herein, Ag@pyrenecarboxaldehyde nanocapsules (Ag@Pyc nanocapsules) as emitters were prepared to construct an ultrasensitive electrochemiluminescence (ECL) biosensor for the detection of the human apurinic/apyrimidinic endonuclease1 (APE1) activity. Ag nanoparticles on the surface of Pyc nanocapsules as coreaction accelerators could significantly promote coreactant peroxydisulfate (S₂O₈²–) to gener ...
DOI:
10.1021/acs.analchem.2c02122

83. Agarose-Droplet-Based Digital LAMP Assay for Counting Virus DNA in Single-Particle ICP-MS

Author:
Xiao Yin; Beibei Chen; Man He; Bin Hu
Source:
Analytical chemistry 2022 v.94 no.17 pp. 6582-6590
ISSN:
1520-6882
Subject:
DNA; Hepatitis B virus; agarose; analytical chemistry; atomic absorption spectrometry; droplets; loop-mediated isothermal amplification; microparticles; polystyrenes; viruses
Abstract:
... Inductively coupled plasma mass spectrometry (ICP-MS) has emerged as a promising analytical platform for the quantification of biomolecules using elemental tags; however, absolute quantification at extremely low concentrations by ICP-MS without a calibration curve remains challenging. Here, we developed a digital loop-mediated isothermal amplification (LAMP) assay for counting hepatitis B virus (H ...
DOI:
10.1021/acs.analchem.2c00427

84. Age prediction of green turtles with an epigenetic clock

Author:
Benjamin Mayne; Walter Mustin; Vandanaa Baboolal; Francesca Casella; Katia Ballorain; Mathieu Barret; Mathew A. Vanderklift; Anton D. Tucker; Darren Korbie; Simon Jarman; Oliver Berry
Source:
Molecular ecology resources 2022 v.22 no.6 pp. 2275-2284
ISSN:
1755-098X
Subject:
Chelonia mydas; DNA; DNA methylation; data collection; ecology; epigenetics; fauna; life history; mark-recapture studies; models; prediction; sea turtles; wild animals; wildlife
Abstract:
... Age is a fundamental life history attribute that is used to understand the dynamics of wild animal populations. Unfortunately, most animals do not have a practical or nonlethal method to determine age. This makes it difficult for wildlife managers to carry out population assessments, particularly for elusive and long‐lived fauna such as marine turtles. In this study, we present an epigenetic clock ...
DOI:
10.1111/1755-0998.13621

85. Aggregation-induced emission by sequence-selective assembly of cyanolated distyrylbenzene in supramolecular DNA architectures

Author:
Hülya Ucar; Hans-Achim Wagenknecht
Source:
Chemical communications 2022 v.58 no.44 pp. 6437-6440
ISSN:
1364-548X
Subject:
DNA; architecture; binding sites; chemical communication; dyes; emissions; harvesting; nucleotide sequences; sequence analysis
Abstract:
... Cyanolated distyrylbenzene conjugated to 2′-deoxyuridine is a new building block for supramolecular DNA architectures combining aggregation-induced emission and sequence-selective binding. A high number of binding sites at the DNA template are occupied by cyanolated distyrylbenzenes. Light can be harvested in this assembly and transferred to terminal Atto dyes. Mixed DNA architectures with perylen ...
DOI:
10.1039/d2cc01161a

86. Agricultural land‐use favours Mucoromycotinian, but not Glomeromycotinian, arbuscular mycorrhizal fungi across ten biomes

Author:
Felipe E. Albornoz; Megan H. Ryan; Gary D. Bending; Sally Hilton; Ian A. Dickie; Deirdre B. Gleeson; Rachel J. Standish
Source:
The new phytologist 2022 v.233 no.3 pp. 1369-1382
ISSN:
0028-646X
Subject:
DNA; Endogonales; agroecosystems; community structure; endophytes; farms; fine roots; land use; phylogeny; rain; soil biota; soil pH; temperature; vesicular arbuscular mycorrhizae
Abstract:
... Globally, agricultural land‐use negatively affects soil biota that contribute to ecosystem functions such as nutrient cycling, yet arbuscular mycorrhizal fungi (AMF) are promoted as essential components of agroecosystems. Arbuscular mycorrhizal fungi include Glomeromycotinian AMF (G‐AMF) and the arbuscule‐producing fine root endophytes, recently re‐classified into the Endogonales order within Muco ...
DOI:
10.1111/nph.17780

87. Alaskan palaeosols in modern times: Deciphering unique microbial diversity within the late-Holocene

Author:
Robyn A Barbato; Robert M Jones; Thomas A Douglas; Julie Esdale; Karen Foley; Edward J Perkins; Shelby Rosten; Natàlia Garcia-Reyero
Source:
Holocene 2022 v.32 no.9 pp. 909-923
ISSN:
1477-0911
Subject:
Archaea; Chloroflexi; DNA; Holocene epoch; Proteobacteria; Verrucomicrobia; climate; community structure; functional diversity; paleosolic soil types; species diversity; vegetation; Alaska
Abstract:
... The Holocene Epoch (11,700 years ago to the present) marks the development of the present-day boreal ecosystems in interior Alaska. The composition and genetics of soil microbes have the potential to alter how nutrient cycling and vegetation respond to warming and cooling events, but very little is known about how boreal soils have varied over time. Here, we use DNA sequencing on both modern soils ...
DOI:
10.1177/09596836221101249

88. Albicanol modulates oxidative stress and the p53 axis to suppress profenofos induced genotoxicity in grass carp hepatocytes

Author:
Xuan Lihui; Qiu Xiaojie; Yu Hao; Chu Jialiang; Guo Jinming; Chang Ying
Source:
Fish & shellfish immunology 2022 v.122 pp. 325-333
ISSN:
1050-4648
Subject:
Ctenopharyngodon idella; DNA; DNA damage; antioxidants; cell cycle; cyclin-dependent kinase; cyclins; fish; genotoxicity; heavy metals; hepatocytes; immunology; models; oxidative stress; profenofos; sesquiterpenoids; shellfish; tail
Abstract:
... The organophosphorus pesticide profenofos (PFF) is widely used as an environmental contaminant, and it can remain in water bodies causing serious harm to aquatic organisms. Albicanol is a sesquiterpenoid with potent antioxidant and antagonistic activities against heavy metal toxicity. However, the mechanism of PFF induced genotoxicity in fish hepatocytes and the role Albicanol can play in this pro ...
DOI:
10.1016/j.fsi.2022.02.002

89. Alexandriicola marinus gen. nov., sp. nov., a new member of the family Rhodobacteraceae isolated from marine phycosphere

Author:
Wen-Zhuo Zhu; Hui-Min Gao; Ya-Ming Ge; Jun Dai; Xi Yang; Xiao-Ling Zhang; Qiao Yang
Source:
Antonie van Leeuwenhoek 2022 v.115 no.4 pp. 473-486
ISSN:
0003-6072
Subject:
Alexandrium catenella; DNA; Rhodobacteraceae; biosynthesis; exopolysaccharides; microalgae; microorganisms; monophyly; new genus; phenotype; phosphatidylcholines; phosphatidylethanolamines; secondary metabolites; toxicity; ubiquinones
Abstract:
... Two yellow-pigmented bacterial strains, LZ-14 ᵀ and ABI-LZ29, were isolated from the cultivable phycosphere microbiota of the highly toxic marine dinoflagellate Alexandrium catenella LZT09 and demonstrated obvious microalgae growth-promoting potentials toward the algal host. To elucidate the taxonomic status of the two bioactive bacterial strains, they were subjected to a polyphasic taxonomic char ...
DOI:
10.1007/s10482-022-01710-2

90. Alkaline lysis-recombinase polymerase amplification combined with CRISPR/Cas12a assay for the ultrafast visual identification of pork in meat products

Author:
Gang Zhao; Jin Wang; Chanyu Yao; Peichun Xie; Xiangmei Li; Zhenlin Xu; Yanping Xian; Hongtao Lei; Xing Shen
Source:
Food chemistry 2022 v.383 pp. 132318
ISSN:
0308-8146
Subject:
DNA; beef; detection limit; equipment; food chemistry; human resources; pork; quantitative polymerase chain reaction; rapid methods; raw meat; recombinase polymerase amplification; swine
Abstract:
... The “Horsemeat Scandal” makes people pay more attention to the meat authenticity. However, expensive equipment, complicated operations, and professional personnel of current methods limit their field testing. In this study, CRISPR/Cas12a combined with recombinase polymerase amplification was used to establish a sensitive and rapid detection method for pig-derived component. The detection limit can ...
DOI:
10.1016/j.foodchem.2022.132318

91. Alkyne-Tagged Raman Probes for Local Environmental Sensing by Hydrogen–Deuterium Exchange

Author:
Xiaotian Bi; Kun Miao; Lu Wei
Source:
Journal of the American Chemical Society 2022 v.144 no.19 pp. 8504-8514
ISSN:
1520-5126
Subject:
Americans; DNA; alkynes; cellular microenvironment; exhibitions; hydrogen; image analysis; journals
Abstract:
... Alkyne-tagged Raman probes have shown high promise for noninvasive and sensitive visualization of small biomolecules to understand their functional roles in live cells. However, the potential for alkynes to sense cellular environments that goes beyond imaging remains to be further explored. Here, we report a general strategy for Raman imaging-based local environment sensing by hydrogen–deuterium e ...
DOI:
10.1021/jacs.2c01991

92. Allele frequencies of 15 autosomal STR loci among 540 Myanmarese individuals in Thailand

Author:
Tayawee Romgaew; Sunisa Aobaom
Source:
Forensic science international 2022 v.6 pp. 100282
ISSN:
2665-9107
Subject:
DNA; alleles; databases; forensic sciences; gender; heterozygosity; nationalities and ethnic groups; paternity; police; probability; statistical analysis; Thailand
Abstract:
... Allele frequencies of 15 autosomal STR loci were established from 540 unrelated Myanmarese individuals using the Thailand Office of Police Forensic Science database. The DNA profiles were provided anonymized with only nationality and gender acknowledged. The allele frequencies were calculated under Hardy-Weinberg equilibrium. The range of allele frequencies was 0.0009–0.5389. Heterozygosity ranged ...
DOI:
10.1016/j.fsir.2022.100282

93. Allopolyploidization increases genetic recombination in the ancestral diploid D genome during wheat evolution

Author:
Hongshen Wan; Jun Li; Shengwei Ma; Fan Yang; Liang Chai; Zehou Liu; Qin Wang; Zongjun Pu; Wuyun Yang
Source:
crop journal 2022 v.10 no.3 pp. 743-753
ISSN:
2214-5141
Subject:
Aegilops tauschii; DNA; Triticum turgidum; artificial selection; diploidy; domestication; evolution; genes; genetic recombination; heterosis; hexaploidy; progeny; segregation distortion; tetraploidy; wheat
Abstract:
... Genetic recombination produces new allelic combinations, thereby introducing variation for domestication. Allopolyploidization has increased the evolutionary potential of hexaploid common wheat by conferring the advantages of heterosis and gene redundancy, but whether a relationship exists between allopolyploidization and genetic recombination is currently unknown. To study the impact of allopolyp ...
DOI:
10.1016/j.cj.2021.09.002

94. Alobar Holoprosencephaly in an Aborted American Quarter Horse Fetus

Author:
Luan Cleber Henker; Marina Paula Lorenzett; Manoela Marchezan Piva; Júlia Gabriela Wronski; Danilo Giorgi Abranches de Andrade; Alexandre Secorun Borges; David Driemeier; José Paes Oliveira-Filho; Saulo Petinatti Pavarini
Source:
Journal of equine veterinary science 2022 v.112 pp. 103898
ISSN:
0737-0806
Subject:
DNA; Quarter Horse; abnormal development; cortex; exons; fetus; foals; humans; liver; necropsy; nose; phenotype; veterinary medicine
Abstract:
... Holoprosencephaly is a central nervous system malformation, characterized by incomplete or total lack of division of prosencephalon hemispheres, which is commonly accompanied by craniofacial malformations. A 9-month-gestation aborted American Quarter Horse fetus was submitted for postmortem examination. The fetus lacked haircoat and had severe facial malformations including marked shortening/absen ...
DOI:
10.1016/j.jevs.2022.103898

95. Aloe vera gel attenuates acetic acid-induced ulcerative colitis in adult male Wistar rats

Author:
W.O. Babalola; D.A. Ofusori; P. Awoniran; B.A. Falana
Source:
Toxicology reports 2022 v.9 pp. 640-646
ISSN:
2214-7500
Subject:
Aloe vera; DNA; acetic acid; adults; catheters; colon; dexamethasone; disease progression; dose response; etiology; gels; histology; inflammation; males; mucosa; nitric oxide; pediatrics; rectum; therapeutics; toxicology; ulcerative colitis
Abstract:
... Ulcerative colitis is a disease of undetermined etiology and treatment. It affects the colon and rectum and typically involves the mucosa, manifesting as continuous areas of inflammation and ulceration. Aloe gel contains more than a hundred potentially active constituents of different classes. This study investigated the effect of aloe gel on experimentally-induced ulcerative colitis. Male Wistar ...
DOI:
10.1016/j.toxrep.2022.03.048

96. Alteration of the expression of sirtuins and var genes by heat shock in the malaria parasite Plasmodium falciparum

Author:
Linda O. Anagu; David R. Hulse; Paul D. Horrocks; Srabasti J. Chakravorty
Source:
Molecular and biochemical parasitology 2022 v.248 pp. 111458
ISSN:
0166-6851
Subject:
Plasmodium falciparum; blood; epigenetics; fever; gene expression; heat stress; lactic acid; malaria; parasites; parasitology; patients; sirtuins; trophozoites; virulence; Gambia; Kenya
Abstract:
... In Plasmodium falciparum the monoallelic expression of var virulence genes is regulated through epigenetic mechanisms. A study in the Gambia showed that an increase in var genes commonly expressed in patients with severe malaria is associated with fever and high blood lactate. A strong association was demonstrated between the upregulation of PfSir2A and group B var genes. A subsequent study in Ken ...
DOI:
10.1016/j.molbiopara.2022.111458

97. Amblyomma ticks in animal carcasses hunted in Mapinguari National Park, Western Amazon, Brazil: New records on species and host-parasite relationships

Author:
Ivaneide Nunes da Costa; Marcela Alvares Oliveira; Paula Frassinetti Medeiros de Paulo; Angélica Lorena Pereira Mendes Carioca; Marcos Valério Garcia; André de Abreu Rangel Aguirre; Jansen Fernandes de Medeiros
Source:
Ticks and tick-borne diseases 2022 v.13 no.5 pp. 101973
ISSN:
1877-959X
Subject:
Amblyomma triste; DNA; Dasypus; Pecari tajacu; Tapirus terrestris; fauna; genes; national parks; parasitism; ticks; Amazonia; Brazil
Abstract:
... Ticks are obligatory parasites and transmit several pathogens to animals and humans. This study aimed to report the parasitism of ticks on carcasses of animals hunted by hunters from communities near Mapinguari National Park, at the shared border of Amazonas and Rondonia states, northern Brazil. The ticks were collected from heads of carcasses of the following animals: South American tapir (Tapiru ...
DOI:
10.1016/j.ttbdis.2022.101973

98. Ameliorative effect of Halopteris filicina extracts on growth parameters and genomic DNA template stability of tomato (Solanum lycopersicum) under lead chloride stress

Author:
Dilek Unal; Gulcin Sevim; Gokay Varis; Inci Tuney-Kizilkaya; Bengu Turkyilmaz Unal; Munir Ozturk
Source:
Crop & pasture science 2022 v.73 no.8 pp. 917-926
ISSN:
1836-0947
Subject:
DNA; Halopteris; Solanum lycopersicum; chlorides; genomics; growth retardation; lead; leaves; lipid peroxidation; macroalgae; pastures; root growth; seedlings; tomatoes; toxicity; viability
Abstract:
... Lead is a toxic element that accumulates in agricultural soils through various anthropogenic sources. It inhibits the growth and development of plants and causes mutations in DNA. Macroalgae such as Halopteris filicina contain multifunctional components that may improve plant tolerance to lead stress. In this study, seeds of tomato (Solanum lycopersicum) were subjected to six treatments comprising ...
DOI:
10.1071/CP21455

99. Ameliorative effects of the phytochemicals in dates (Phoenix dactylifera) against the toxicological changes induced by fipronil in male albino rats

Author:
Hossam El Din H. Abdelhafez; Amr A. Abdallah; Asmaa A. El-Dahshan; Yasser A. Abd El-baset; Osama M. Morsy; Mohamed Bedair M. Ahmed
Source:
Toxicology 2022 v.480 pp. 153313
ISSN:
0300-483X
Subject:
DNA; Phoenix dactylifera; albino; antioxidant activity; antioxidants; bone marrow; catalase; fipronil; genotoxicity; glutathione; histopathology; kidneys; lethal dose 50; lipid peroxidation; liver; males; malondialdehyde; oxidation; oxidative stress; phytochemicals; protective effect; superoxide dismutase; toxicology
Abstract:
... Scientific evidence has shown that fipronil induces oxidative stress and genotoxicity. Our study aimed to evaluate the potential oxidation in redox parameters and DNA, as well as determine the protective effect of date extract of increasing resistance to cellular damage. 30 Male albino rats were divided into six groups (n = 5): 1) control group; 2) treatment group with date extract (1 g/kg B.W.); ...
DOI:
10.1016/j.tox.2022.153313

100. Amine functionalized carbon quantum dots from paper precursors for selective binding and fluorescent labelling applications

Author:
Varsha Lisa John; Francis Joy; Amy Jose Kollannoor; Kuruvilla Joseph; Yamuna Nair; Vinod T. P.
Source:
Journal of colloid and interface science 2022 v.617 pp. 730-744
ISSN:
0021-9797
Subject:
DNA; calves; cellulose; fluorescence; paper; silane
Abstract:
... We report a novel synthesis route for preparing carbon quantum dots (CQDs) of customized surface functionality from readily available precursors. The synthetic strategy is based on the chemical modification of paper precursors prior to preparing CQDs from them. The pre-synthesis modification of paper precursors with (3-Aminopropyl) triethoxy silane (APTES) enabled us to synthesize CQDs with amine ...
DOI:
10.1016/j.jcis.2022.03.070