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1. "Electrokinetic Analyte Transport Assay" for α-fetoprotein immunoassay integrates mixing, reaction and separation on-chip

Author:
Kawabata, Tomohisa; Wada, Henry G.; Watanabe, Mitsuo; Satomura, Shinji
Source:
Electrophoresis 2008 v.29 no.7 pp. 1399-1406
ISSN:
0173-0835
Subject:
DNA; antibodies; antigen-antibody complex; blood; electrophoresis; immunoassays; laminar flow; liver neoplasms; mixing
Abstract:
... A rapid and highly sensitive CE immunoassay method integrating mixing, reaction, separation, and detection on-chip is described for the measurement of α-fetoprotein (AFP), a liver cancer marker in blood. Antibody-binding reagents, consisting of 245-bp DNA coupled anti-AFP WA1 antibody (DNA-WA1) and HiLyte dye-labeled anti-AFP WA2 antibody (HiLyte-WA2), and AFP-containing sample were filled into ad ...
DOI:
10.1002/elps.200700898

2. "MALDI-CSI": A proposed method for the tandem detection of human blood and DNA typing from enhanced fingermarks

Author:
Katie Kennedy; Ravell Bengiat; Cameron Heaton; Yael Herman; Carla Oz; Michal Levin Elad; Laura Cole; Simona Francese
Source:
Forensic science international 2021 v.323 pp. 110774
ISSN:
0379-0738
Subject:
DNA; blood; desorption; digestion; forensic sciences; hemoglobin; humans; mass spectrometry; peptides; proteomics
Abstract:
... Matrix Assisted Laser Desorption Ionization Mass Spectrometry Profiling and Imaging (MALDI MSP and MALDI MSI), in combination with bottom up proteomics, have proven to successfully detect and map blood-derived peptide signatures in blood fingermarks, with high specificity and compatibility with a number of blood enhancement techniques (BET). In the present study, the application of MALDI MSP and M ...
DOI:
10.1016/j.forsciint.2021.110774

3. "Reverse degradomics", monitoring of proteolytic trimming by multi-CE and confocal detection of fluorescent substrates and reaction products

Author:
Piccard, Helene; Hu, Jialiang; Fiten, Pierre; Proost, Paul; Martens, Erik; Van den Steen, Philippe E.; Van Damme, Jo; Opdenakker, Ghislain
Source:
Electrophoresis 2009 v.30 no.13 pp. 2366-2377
ISSN:
0173-0835
Subject:
proteolysis; screening; bioactive properties; monitoring; protein-glutamine gamma-glutamyltransferase; cell culture; DNA; fluorescence; data collection; proteinases; chemokine CXCL6; fluorescent labeling; amino acids; mice; neoplasm cells; neutrophils; proteinase inhibitors; electrophoresis
Abstract:
... A platform for profiling of multiple proteolytic activities acting on one specific substrate, based on the use of a 96-channel capillary DNA sequencer with CE-LIF of labeled substrate peptides and reaction products is introduced. The approach consists of synthesis of a substrate peptide of interest, fluorescent labeling of the substrate, either aminoterminally by chemical coupling, or carboxytermi ...
DOI:
10.1002/elps.200800698

4. "pp65 antigenemia and real time polymerase chain reaction (PCR) based-study to determine the prevalence of human cytomegalovirus (HCMV) in kidney donors and recipients with follow-up studies."

Author:
Hajib N Madhavan; Moses Y Samson; Murali Ishwarya; Ramanathan Vijayakumar; Malathi Jambulingam
Source:
Virology journal 2010 v.7 no.1 pp. 322
ISSN:
1743-422X
Subject:
DNA; Human betaherpesvirus 5; blood; chi-square distribution; kidney transplant; kidneys; prospective studies; quantitative polymerase chain reaction; virology
Abstract:
... BACKGROUND: The present study was undertaken to determine the rate of occurrence of Human cytomegalovirus (HCMV) among kidney transplant recipients and donors by application of direct detection methods and to understand HCMV infection/disease development among transplanted patients as a prospective study. RESULTS: Peripheral blood samples collected from 76 kidney donors and 76 recipients from Sept ...
DOI:
10.1186/1743-422X-7-322

5. %G+C profiling and cross hybridisation of microbial DNA reveals great variation in below-ground community structure in UK upland grasslands

Author:
Clegg, C.D.; Ritz, K.; Griffiths, B.S.
Source:
Applied soil ecology 2000 v.14 no.2 pp. 125-134
ISSN:
0929-1393
Subject:
chemical composition; upland soils; DNA; guanine; cytosine; soil microorganisms; species diversity; spatial variation; nucleic acid hybridization; grasslands; thermal degradation; grassland soils; United Kingdom

6.   Effects of homobrassinolide in barley callus culture

Author:
A. Temel; N. Gozukirmizi
Source:
Plant, Soil and Environment v.58 no.10 pp. 441-445
ISSN:
1214-1178
Subject:
DNA; DNA methylation; Hordeum vulgare; barley; callus; callus culture; environment; soil; superoxide dismutase; tissue culture
Abstract:
... Thirty-day-old barley (Hordeum vulgare L. cv. Zafer-160) calli were treated with 0, 0.5, 1 µmol/L 22(S),23(S)-Homobrassinolide (HBL) for four weeks. Transfer of calli to hormone-free or HBL-supplemented medium caused an increase in total soluble protein and DNA contents and a decrease in superoxide dismutase (SOD; E.C. 1.15.1.1) activity. 0.5 µmol/L HBL caused the highest protein and D ...
DOI:
10.17221/305/2012-pse

7.  DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa

Author:
P. Prinosilova; R. Rybar; A. Zajicova; J. Hlavicova
Source:
Veterinární medicína v.57 no.3 pp. 133-142
ISSN:
0375-8427
Subject:
DNA; DNA fragmentation; chromatin; cryopreservation; dogs; freeze-thaw cycles; male fertility; semen; sperm motility; spermatozoa; storage time; viability
Abstract:
... Sperm chromatin status in fresh dog semen and the effect of long-term storage of chilled and frozen dog semen on sperm chromatin integrity was assessed by the sperm chromatin structure assay (SCSA). In the first experiment, the chromatin integrity of fresh semen from 60 dogs with differing histories of fertility was compared with other sperm parameters (total sperm count, sperm motility, viability ...
DOI:
10.17221/5853-vetmed

8.  Genetic variation in three paddlefish (Polyodon spathula Walbaum) stocks based on microsatellite DNA analysis

Author:
D. Kaczmarczyk; M. Luczynski; P. Brzuzan
Source:
Czech Journal of Animal Science v.57 no.8 pp. 345-352
ISSN:
1212-1819
Subject:
DNA; Polyodon spathula; adults; animal science; fish; founder effect; genetic distance; genetic variation; heterozygosity; loci; microsatellite repeats; Mississippi; Mississippi River; Poland; Ukraine
Abstract:
... There are two stocks of American paddlefish Polyodon spathula in Poland, one in Pogorze and the other in Wasosze. These stocks were established from small quantities of eggs imported from the USA in 1995. In this study, we examined genetic variation at seven microsatellite loci in adult fish from the two Polish farms and one stock farmed in Gorny Tykich in Ukraine. Our data were compared with thos ...
DOI:
10.17221/6269-cjas

9.  Isolation, cloning and sequence analysis of lactate dehydrogenase gene from Theileria annulata may lead to design of new antitheilerial drugs

Author:
A. Erdemir; M. Aktas; N. Dumanli; D. Turgut-Balik
Source:
Veterinární medicína v.57 no.10 pp. 559-567
ISSN:
0375-8427
Subject:
Brown Swiss; DNA; Protozoa; Theileria annulata; Theileria parva; active sites; animal diseases; antiparasitic agents; blood; cows; introns; lactate dehydrogenase; lymphatic diseases; mechanism of action; morbidity; mortality; sequence analysis; theileriosis
Abstract:
... Theileriosis is a serious animal disease that is transmitted by ticks. Theileria species are tick-borne obligate intracellular protozoan parasites that cause severe and mild infections in their hosts. Two of them, Theileria annulata and Theileria parva, cause lymphoproliferative disease with high mortality and morbidity in cattle commonly known as tropical theileriosis and East Coast fever, respec ...
DOI:
10.17221/6368-vetmed

10.  Leaf spot and dieback of Buxus caused by Cylindrocladium buxicola

Author:
I. Šafránková; L. Holková; M. Kmoch
Source:
Plant Protection Science v.49 no.4 pp. 165-168
ISSN:
1212-2580
Subject:
Buxus sempervirens; Calonectria pseudonaviculata; DNA; azoxystrobin; boxwood blight; conidia; dieback; germination; kresoxim-methyl; leaf blight; leaf spot; mancozeb; mycelium; myclobutanil; penconazole; plant protection
Abstract:
... Leaf blight symptoms were observed on potted box plants (Buxus sempervirens cv. Suffruticosa and B. microphylla) in a nursery in South Moravia in August 2010. These symptoms were suggestive of box blight on Buxus spp. Characteristics of the visual symptoms, microscopic features, and identification of the isolates using DNA sequencing are described. The causal agent was isolated and identified as C ...
DOI:
10.17221/82/2012-pps

11.  Metals as a cause of oxidative stress in fish: a review

Author:
M. Sevcikova; H. Modra; A. Slaninova; Z. Svobodova
Source:
Veterinární medicína v.56 no.11 pp. 537-546
ISSN:
0375-8427
Subject:
DNA; antioxidant activity; aquatic environment; biomarkers; enzyme inactivation; ferritin; ferroxidase; fish; gene expression; homeostasis; lipid peroxidation; metabolism; metallothionein; molecular weight; oxidation; oxidative stress; quantitative analysis; reactive oxygen species; redox potential; toxicity
Abstract:
... This review summarizes the current knowledge on the contribution of metals to the development of oxidative stress in fish. Metals are important inducers of oxidative stress in aquatic organisms, promoting formation of reactive oxygen species through two mechanisms. Redox active metals generate reactive oxygen species through redox cycling, while metals without redox potential impair antioxidant de ...
DOI:
10.17221/4272-vetmed

12.  Rapid sexing of selected Galliformes by polymerase chain reaction

Author:
J. Kalina; J. Mucksová; H. Yan; P. Trefil
Source:
Czech Journal of Animal Science v.57 no.4 pp. 187-192
ISSN:
1212-1819
Subject:
DNA; Gallus gallus; Meleagris gallopavo; Phasianus colchicus; W chromosome; chickens; cost effectiveness; grouse; pheasants; phenotype; polymerase chain reaction; sex determination; wood
Abstract:
... Vent sexing of one-day-old chicks in commercial hatcheries has long been common practice and can be highly accurate. However, there are circumstances when this technique is not applicable such as smaller breeds, non-domestic birds, or where is the necessity of precise sexing. In this study we present a simple and reliable method for fast gender determination in selected Galliformes for which pheno ...
DOI:
10.17221/5894-cjas

13.  The effect of high temperature on swine ovarian function in vitro

Author:
AV Sirotkin; M. Kacaniova
Source:
Veterinární medicína v.55 no.8 pp. 377-382
ISSN:
0375-8427
Subject:
DNA; estradiol; genes; heat stress; leptin; progesterone; swine; temperature
Abstract:
... The aim of the present study was to understand the hormonal mechanisms behind the effect of high temperatures on reproductive function. It was proposed that high temperatures can directly alter production of ovarian hormones and/or the response of ovarian cells to hormonal stimulators. To examine this hypothesis, in the 1st series of experiments, we compared the release of progesterone (P4
DOI:
10.17221/2953-vetmed

14.  The structure and role of mammalian sperm RNA: a review

Author:
D. Bukowska; B. Kempisty; H. Piotrowska; P. Sosinska; M. Wozna; S. Ciesiolka; P. Antosik
Source:
Veterinární medicína v.58 no.2 pp. 57-64
ISSN:
0375-8427
Subject:
DNA; chromatin; embryogenesis; genome; haploidy; histones; males; mammals; messenger RNA; microarray technology; oocytes; protamines; spermatogenesis; spermatozoa
Abstract:
... The main role of sperm is the delivery of the paternal genome into the oocyte during fertilisation. However, several lines of evidence have indicated that mammalian spermatozoa contribute more than just their DNA, namely, they also deliver a large range of RNA molecules. Microarray analysis has revealed a complex population of 3000 different kinds of messenger RNA that are delivered to oocytes by ...
DOI:
10.17221/6696-vetmed

15. 'Candidatus Lumbricincola', a novel lineage of uncultured Mollicutes from earthworms of family Lumbricidae

Author:
Nechitaylo, Taras Y.; Timmis, Kenneth N.; Golyshin, Peter N.
Source:
Environmental microbiology 2009 v.11 no.4 pp. 1016-1026
ISSN:
1462-2912
Subject:
Clostridium; DNA; Lumbricidae; Phytoplasma; clones; digestive system; earthworms; fluorescence in situ hybridization; host specificity; nucleotide sequences; ribosomal RNA; sequence analysis; single-stranded conformational polymorphism; Germany; Russia
Abstract:
... Molecular signatures of new, as yet uncultured mollicute-like organisms (MLOs) have been detected in total rRNA and DNA extracted from tissues, gut contents and casts of four species of the earthworm family Lumbricidae. The MLO 16S rRNA sequences exhibited low identity to those of known Mollicutes species and formed a monophyletic cluster distantly affiliated to the 'Candidatus Bacilloplasma' (84. ...
DOI:
10.1111/j.1462-2920.2008.01837.x
PubMed:
19396950

16. 'Jumping Jack': Genomic microsatellites underscore the distinctiveness of closely related Pseudoperonospora cubensis and Pseudoperonospora humuli and provide new insights into their evolutionary past

Author:
Nowicki Marcin; Guerry Denita; Trigiano Robert N.; Boggess Sarah L.; Kanetis Loukas; Wadl Phillip A.; Ojiambo Peter S.; Cubeta Marc A.; Spring Otmar; Thines Marco; Runge Fabien; Scheffler Brian E.
Source:
Molecular plant pathology 2021 v.12 no. pp. 686759
ISSN:
1464-6722
Subject:
Cucurbitaceae; DNA; Pseudoperonospora cubensis; Pseudoperonospora humuli; gene flow; genes; genetic variation; genomics; herbaria; microsatellite repeats; mitochondria; plant pathology; population structure
Abstract:
... Downy mildews caused by obligate oomycetes result in severe crop losses worldwide. Among these pathogens, Pseudoperonospora cubensis and P. humuli, two closely related species, adversely affect cucurbits and hops, respectively. Discordant hypotheses concerning their taxonomic relationships have been proposed based on phytopathological evidence and gene sequences of few individuals, but population ...
Handle:
10113/7482132
DOI:
10.3389/fmicb.2021.686759

17. 'Lysobacter enzymogenes ssp. cookii ' Christensen 1978 should be recognized as an independent species, Lysobacter cookii sp. nov

Author:
Kawamura, Yoshiaki; Tomida, Junko; Morita, Yuji; Naka, Takashi; Mizuno, Seiko; Fujiwara, Nagatoshi
Source:
FEMS microbiology letters 2009 v.298 no.1 pp. 118-123
ISSN:
0378-1097
Subject:
DNA; Lysobacter capsici; Lysobacter enzymogenes; fatty acid composition; genome; new species
Abstract:
... 'Lysobacter enzymogenes ssp. cookii' was proposed by Christensen and Cook in 1978; however, this subspecies name has not been cited in the Approved Lists of Bacterial Names and therefore the nomenclature has not been validated. In our genetic approach to clarify the relationships of the designated type strain of 'L. enzymogenes ssp. cookii' PAGU 1119 (GenBank accession number ATCC29488) within the ...
DOI:
10.1111/j.1574-6968.2009.01703.x
PubMed:
19659731

18. The 'kinetochore maintenance loop'--The mark of regulation?

Author:
Brown, William R.A.; Xu, Zheng-yao
Source:
BioEssays 2009 v.31 no.2 pp. 228-236
ISSN:
0265-9247
Subject:
DNA; chromatids; cohesion; epigenetics; humans; interphase; kinetochores; metaphase; mitosis; nucleotide sequences
Abstract:
... Kinetochores can form and be maintained on DNA sequences that are normally non-centromeric. The existence of these so-called neo-centromeres has posed the problem as to the nature of the epigenetic mechanisms that maintain the centromere. Here we highlight results that indicate that the amount of CENP-A at human centromeres is tightly regulated. It is also known that kinetochore assembly requires ...
DOI:
10.1002/bies.200800118

19. (2S)-Naringenin from Typha angustata inhibits vascular smooth muscle cell proliferation via a G₀/G₁ arrest

Author:
Jung-Jin Lee; Hyoseok Yi; In-Su Kim; Yohan Kim; Nguyen Xuan Nhiem; Young Ho Kim; Chang-Seon Myung
Source:
Journal of ethnopharmacology 2012 v.139 no.3 pp. 873-878
ISSN:
0378-8741
Subject:
DNA; Oriental traditional medicine; Typha; antibacterial properties; atherosclerosis; cell cycle; cell proliferation; flow cytometry; immunoblotting; myocardial infarction; myocytes; phosphatidylinositol 3-kinase; phosphorylation; platelet-derived growth factor; protein kinases; smooth muscle; viability
Abstract:
... ETHNOPHARMACOLOGICAL RELEVANCE: Typha angustata is used in traditional Chinese medicine for a variety of clinical disorders. Its pharmacological actions include beneficial effects on hyperlipidemia and myocardial infarction, as well as labor-inducing and antibacterial effects. AIM OF THE STUDY: We investigated the mechanism underlying the ability of (2S)-naringenin, an active compound from Typha a ...
DOI:
10.1016/j.jep.2011.12.038

20. (E)-2,4-Bis(p-hydroxyphenyl)-2-butenal enhanced TRAIL-induced apoptosis in ovarian cancer cells through downregulation of NF-κB/STAT3 pathway

Author:
Seung Hee Cho; Mi Hee Park; Hee Peom Lee; Myong Ki Back; Ha Chang Sung; Hee Won Chang; Joo Hwan Kim; Heon-Sang Jeong; Sang Bae Han; Jin Tae Hong
Source:
Archives of pharmacal research 2014 v.37 no.5 pp. 652-661
ISSN:
0253-6269
Subject:
transcription factor NF-kappa B; Maillard reaction; humans; non-specific protein-tyrosine kinase; prognosis; DNA; proteolysis; signal transduction; ovarian neoplasms; antineoplastic agents; neoplasm cells; phosphorylation; animal ovaries; apoptosis; caspase-3
Abstract:
... Ovarian cancer is a cancerous growth arising from the ovary and with poor prognosis that usually have resistant to all currently available treatments. Whether (E)-2,4-bis(p-hydroxyphenyl)-2-butenal (butenal) synthesized by Maillard reaction from fructose–tyrosine, has potential therapeutic activity against human ovarian cancer was investigated using two ovarian cancer cell lines (PA-1, SK-OV-3). W ...
DOI:
10.1007/s12272-013-0326-9

21. The (TTTA) n polymorphism in intron 4 of CYP19 and the polycystic ovary syndrome risk in a Chinese population

Author:
P. Xu; X. L. Zhang; G. B. Xie; C. W. Zhang; S. M. Shen; X. X. Zhang; Y. X. Cao; W. J. Wang; Y. N. Che; Y. J. Xia; X. K. Wu; L. Yi; Q. Gao; Y. Wang
Source:
Molecular biology reports 2013 v.40 no.8 pp. 5041-5047
ISSN:
0301-4851
Subject:
DNA; alleles; animal ovaries; blood serum; capillary electrophoresis; case-control studies; genotype; introns; microsatellite repeats; patients; polycystic ovary syndrome; polymerase chain reaction; risk; steroid hormones
Abstract:
... Polycystic ovary syndrome (PCOS) is a heterogeneous disease with a strong genetic origin, but the specific determinants are still unknown. The aim of this study was to investigate the association between the (TTTA) ₙ polymorphism in intron 4 of CYP19 and the PCOS risk in a Chinese population. We performed a case–control study which involved 222 PCOS patients and 281 controls. The fluorescent-label ...
DOI:
10.1007/s11033-013-2605-4

22. (−)-Anonaine Induces DNA Damage and Inhibits Growth and Migration of Human Lung Carcinoma H1299 Cells

Author:
Chen, Bing-Hung; Chang, Hsueh-Wei; Huang, Hsuan-Min; Chong, Inn-Wen; Chen, Jia-Shing; Chen, Chung-Yi; Wang, Hui-Min
Source:
Journal of agricultural and food chemistry 2011 v.59 no.6 pp. 2284-2290
ISSN:
0021-8561
Subject:
DNA; DNA damage; anticarcinogenic activity; cell cycle; cell growth; chemoprevention; herbal medicines; humans; lung neoplasms
Abstract:
... The anticancer effects of (−)-anonaine were investigated in this current study. (−)-Anonaine at concentration ranges of 50−200 μM exhibited significant inhibition to cell growth and migration activities on human lung cancer H1299 cells at 24 h, albeit cell cycle analyses showed that (−)-anonaine at the above concentration ranges did not cause any significant changes in cell-cycle distributions. Si ...
DOI:
10.1021/jf103488j

23. The -866G/A polymorphism in the promoter of the UCP2 gene is associated with risk for type 2 diabetes and with decreased insulin levels

Author:
Panneerselvam Gomathi; Apurwa P. Samarth; Nancy Bright Arul Joseph Raj; Sundaresan Sasikumar; Ponniah Senthil Murugan; Sivagnanam Nallaperumal; Govindan Sadasivam Selvam
Source:
Gene 2019 v.701 pp. 125-130
ISSN:
0378-1119
Subject:
alleles; blood glucose; blood lipids; blood serum; diastolic blood pressure; genotype; genotyping; insulin; insulin secretion; islets of Langerhans; lipid composition; lipid metabolism; noninsulin-dependent diabetes mellitus; odds ratio; oxidative stress; pathogenesis; patients; polymerase chain reaction; restriction fragment length polymorphism; risk factors
Abstract:
... Oxidative stress and impaired insulin secretion is an underlying major risk factor for the development of type 2 diabetes (T2D). Uncoupling protein-2 (UCP2) is involved in the regulation of reactive oxygen species production, insulin secretion, and lipid metabolism. Based on this we aimed to find an association of UCP2 (G-866A) polymorphism with the risk of T2D in South Indian population.A total o ...
DOI:
10.1016/j.gene.2019.03.041

24. 025 Freezing mammalian semen: If only all sperm were equal!

Author:
William Holt
Source:
Cryobiology 2013 v.67 no.3 pp. 405
ISSN:
0011-2240
Subject:
DNA; Macropodidae; Sarcophilus harrisii; anthropogenic activities; breeding programs; chromatin; cryopreservation; cryoprotectants; cysteine; death; deforestation; disulfide bonds; freezing; gene banks; gene pool; glycerol; inbreeding; interspecific variation; males; mammals; oviductal isthmus; plasma membrane; semen; spermatozoa; thawing; viability; wildlife; wildlife management
Abstract:
... Wildlife extinctions are thought to be occurring more rapidly than at any time in the history of the earth, mainly because of human activities. These include agriculture, urbanization, deforestation and a myriad other processes. While it is impractical to think that reproductive technologies could be used on a grand scale to help save large numbers of species from extinction, the judiciously targe ...
DOI:
10.1016/j.cryobiol.2013.09.031

25. 076 Cryo-preanalytics: From biospecimen research to quality control

Author:
Olga Kofanova
Source:
Cryobiology 2013 v.67 no.3 pp. 419
ISSN:
0011-2240
Subject:
DNA; blood; cold; cold storage; freezing; metabolomics; phosphoproteins; quality control; research projects; temperature
Abstract:
... Biospecimen Research includes biospecimen collection, processing and storage method validation, biospecimen stability studies, biospecimen quality control and research on the impact of preanalytical variables on the quality of the samples. Hypothermic conditions and cryogenic temperatures are part of the critical preanalytical variables. The presentation will show how biospecimen research bridges ...
DOI:
10.1016/j.cryobiol.2013.09.082

26. 081 Ambient temperature stabilization of nucleic acids in human blood

Author:
Rolf Muller
Source:
Cryobiology 2013 v.67 no.3 pp. 420-421
ISSN:
0011-2240
Subject:
DNA; RNA; ambient temperature; biomedical research; blood; cold; diagnostic techniques; freezers; genotyping; humans; hydrolysis; precision medicine; saliva; shipping; transcriptome
Abstract:
... Biospecimen stabilization during collection and transport is of critical importance in the fields of biomedical research and molecular diagnostics. The emergence of personalized medicine has placed an even greater emphasis on the significance of protecting the quality and reliability of clinical samples. The current methods for blood and saliva collection depend heavily on cold-chain logistics and ...
DOI:
10.1016/j.cryobiol.2013.09.087

27. 086 Optimal preservation of DNA Integrity in cat germinal vesicles after microwave-assisted dehydration and supra-zero temperature storage

Author:
Pei-Chih Lee; E. Paramore; M. Van Vorst; L. Weng; D.E. Wildt; G.D. Elliott; P. Comizzoli
Source:
Cryobiology 2013 v.67 no.3 pp. 422
ISSN:
0011-2240
Subject:
DNA; DNA damage; DNA fragmentation; adults; air drying; ambient temperature; animal models; bags; cats; chromatin; equilibrium moisture content; filters; genome; germinal vesicle; glass transition temperature; herring; microwave treatment; staining; storage; storage temperature; titration; trehalose
Abstract:
... The germinal vesicle (GV) is an alternative target to the whole oocyte for exploring new genome preservation approaches at supra-zero temperatures. Using the domestic cat model, we have demonstrated that the GV can survive simple air-drying and then be hydrated to reconstitute a viable oocyte (Graves-Herring et al., 2013). However, this method can lead to variable DNA damage due to uncontrolled wa ...
DOI:
10.1016/j.cryobiol.2013.09.092

28. 094 Application of the Kwei equation for predicting the Tg of stabilizing formulations containing sugars and salts

Author:
Lindong Weng; Ranganathan Vijayaraghavan; Douglas R. MacFarlane; Gloria D. Elliott
Source:
Cryobiology 2013 v.67 no.3 pp. 424
ISSN:
0011-2240
Subject:
DNA; choline; cytochrome c; data collection; differential scanning calorimetry; drugs; equations; foods; glass; glass transition temperature; interleukin-2; lysozyme; models; phosphates; prediction; shelf life; stabilizers; storage temperature; sucrose; thermal stability; trehalose
Abstract:
... Sugars such as trehalose and sucrose have been used in numerous formulations to improve the shelf-life of products ranging from foods to pharmaceuticals and more recently they have also been used as a key protectant for preserving cell-based products. Disaccharides are known to have high glass transition temperatures and thus can immobilize molecules at high storage temperatures. Frequently these ...
DOI:
10.1016/j.cryobiol.2013.09.100

29. 1,3-Di-n-butylimidazolium tribromide [BBim]Br3: An efficient recyclable catalyst mediated synthesis of N-substituted azepines and their biological evaluation-interaction study with human serum albumin

Author:
Swetha K. Maddili; Rakhi Chowrasia; Vijaya Kumar Kannekanti; Himabindu Gandham
Source:
Journal of photochemistry and photobiology 2018 v.178 pp. 101-107
ISSN:
1011-1344
Subject:
DNA; acetylene; antibacterial properties; aromatic amines; calves; catalysts; drugs; fluconazole; fungi; human serum albumin; ionic liquids; thymus gland; ultraviolet-visible spectroscopy; wavelengths
Abstract:
... A majority of previously reported methods suffer from insufficient yields as well as more complicated experimental procedures, a smaller amount of isolated yields involving time-consuming and tiresome work-up with the use of metal catalyst and restricted scope of substrates. To overcome these issues, an environmentally benign, ionic liquid endorsed multi-component protocol to N-substituted azepine ...
DOI:
10.1016/j.jphotobiol.2017.10.028

30. 1,8-Cineole inhibits root growth and DNA synthesis in the root apical meristem ofBrassica campestris L.

Author:
Ritsuko Koitabashi; Takeshi Suzuki; Tamotsu Kawazu; Atsushi Sakai; Haruko Kuroiwa; Tsuneyoshi Kuroiwa
Source:
Journal of plant research 1997 v.110 no.1 pp. 1-6
ISSN:
0918-9440
Subject:
DNA; allelopathy; antibodies; apical meristems; cell nucleus; cineole; fluorescence microscopy; germination; growth retardation; hypocotyls; mitosis; organelles; root growth; roots; seedlings; thymidine
Abstract:
... 1,8-cineole is a volatile growth inhibitor produced bySalvia species. We examined the effect of this allelopathic compound on the growth of other plants usingBrassica campestris as the test plant. Cineole inhibited germination and growth ofB. campestris in a dosedependent manner. WhenB. campestris was grown for 5 days with various concentrations of cineole, the length of the roots was found to be ...
DOI:
10.1007/BF02506836

31. 1-MCP treatment enhanced expression of genes controlling endosperm cell division and starch biosynthesis for improvement of grain filling in a dense-panicle rice cultivar

Author:
B.B. Panda; A.K. Badoghar; S. Sekhar; B.P. Shaw; P.K. Mohapatra
Source:
Plant science 2016 v.246 pp. 11-25
ISSN:
0168-9452
Subject:
1-methylcyclopropene; DNA; Oryza sativa; biosynthesis; cultivars; cytokinesis; endopolyploidy; endosperm; ethylene; ethylene production; filling period; flowering; fruits; gene expression; genes; inflorescences; rice; signal transduction; starch
Abstract:
... High ethylene production in dense-panicle rice cultivars impacts grain filling. 1-MCP (ethylene action inhibitor) treatment increased assimilates partitioning, cell number and size and expression of starch synthesizing enzyme genes of developing caryopses mostly in the basal spikelets of panicle at early post-anthesis stage. The gain in cell number was less compared to the increase of size. High e ...
DOI:
10.1016/j.plantsci.2016.02.004

32. 1-Methylcyclopropene (1-MCP) treatment differentially mediated expression of vacuolar processing enzyme (VPE) genes and delayed programmed cell death (PCD) during ripening and senescence of apple fruit

Author:
Jingyi Lv; Yingzhi Zhang; Mingyu Sun; Jingxin Chen; Yonghong Ge; Jianrong Li
Source:
Scientia horticulturae 2023 v.307 pp. 111489
ISSN:
0304-4238
Subject:
1-methylcyclopropene; DNA; Malus domestica; apples; climacteric fruits; electrical conductivity; enzymes; ethylene; firmness; malondialdehyde; programmed cell death; reactive oxygen species; transmission electron microscopy; vacuoles
Abstract:
... The main purpose of this research was to evaluate the impacts of 1-methylcyclopropene (1-MCP) treatment on vacuolar processing enzyme (VPE) genes and programmed cell death (PCD) during ripening and senescence of apple fruit (Malus × domestica Borkh.). The ethylene antagonists 1-MCP (1 μL L⁻¹) was applied to apples after harvest. Both treated and control fruit were stored at 20 ± 2°C. The data show ...
DOI:
10.1016/j.scienta.2022.111489

33. 1.688 g/cm3 satellite‐related repeats: a missing link to dosage compensation and speciation

Author:
Miguel Gallach
Source:
Molecular ecology 2015 v.24 no.17 pp. 4340-4347
ISSN:
0962-1083
Subject:
DNA; Drosophila; X chromosome; hybrids; models
Abstract:
... Despite the important progress that has been made on dosage compensation (DC), a critical link in our understanding of the X chromosome recognition mechanisms is still missing. Recent studies in Drosophila indicate that the missing link could be a family of DNA repeats populating the euchromatin of the X chromosome. In this opinion article, I discuss how these findings add a new fresh twist on the ...
DOI:
10.1111/mec.13335

34. The 1.6Å resolution structure of activated D138L mutant of catabolite gene activator protein with two cAMP bound in each monomer

Author:
Wenbing Tao; Zengqiang Gao; Zhengya Gao; Jiahai Zhou; Zhongxian Huang; Yuhui Dong; Shaoning Yu
Source:
International journal of biological macromolecules 2011 v.48 no.3 pp. 459-465
ISSN:
0141-8130
Subject:
DNA; Escherichia coli; X-ray diffraction; binding sites; crystal structure; cyclic AMP; genes; mutants; nucleotide sequences; proteinases
Abstract:
... The X-ray crystal structure of the cAMP-liganded D138L mutant of Escherichia coli catabolite gene activator protein (CAP) was determined at a resolution of 1.66Å. This high resolution crystal structure reveals four cAMP binding sites in the homodimer. Two anti conformations of cAMPs (anti-cAMP) locate between the β-barrel and the C-helix of each subunit; two syn conformations of cAMPs (syn-cAMP) b ...
DOI:
10.1016/j.ijbiomac.2011.01.009

35. The 1.8-Å Crystal Structure of the N-Terminal Domain of an Archaeal MCM as a Right-Handed Filament

Author:
Yang Fu; Ian M. Slaymaker; Junfeng Wang; Ganggang Wang; Xiaojiang S. Chen
Source:
Journal of Molecular Biology 2014 v.426 pp. 1512-1523
ISSN:
0022-2836
Subject:
DNA; DNA replication; bacteria; crystal structure; genes; proteins
Abstract:
... Mini-chromosome maintenance (MCM) proteins are the replicative helicase necessary for DNA replication in both eukarya and archaea. Most of archaea only have one MCM gene. Here, we report a 1.8-Å crystal structure of the N-terminal MCM from the archaeon Thermoplasma acidophilum (tapMCM). In the structure, the MCM N-terminus forms a right-handed filament that contains six subunits in each turn, with ...
DOI:
10.1016/j.jmb.2013.12.025

36. IL- 10 gene polymorphism c.-592C>A increases HPV infection susceptibility and influences IL-10 levels in HPV infected women

Author:
Fernanda Costa Brandão Berti; Ana Paula Lombardi Pereira; Kleber Paiva Trugilo; Guilherme Cesar Martelossi Cebinelli; Lorena Flor da Rosa Santos Silva; Marcell Alysson Batisti Lozovoy; Andréa Name Colado Simão; Maria Angelica Ehara Watanabe; Karen Brajão de Oliveira
Source:
Infection, genetics, and evolution 2017 v.53 pp. 128-134
ISSN:
1567-1348
Subject:
DNA; alleles; blood sampling; carcinogenesis; enzyme-linked immunosorbent assay; epithelium; heterozygosity; homozygosity; immunosuppression; interleukin-10; patients; polymerase chain reaction; regression analysis; restriction fragment length polymorphism; women
Abstract:
... Interleukin-10 (IL-10) influences HPV infection and viral persistence, favoring cervical immunosuppression and cervical carcinogenesis. IL-10 levels may be influenced by HPV itself and by IL-10 polymorphisms, including rs1800872 (c.-592C>A). Therefore, we evaluated the influence of IL-10 c.-592C>A polymorphism in HPV infection and in IL-10 plasmatic/cervical levels in HPV infected and non-infected ...
DOI:
10.1016/j.meegid.2017.05.020

37. 10-Plex Digital Polymerase Chain Reaction with Four-Color Melting Curve Analysis for Simultaneous KRAS and BRAF Genotyping

Author:
Tatsuo Nakagawa; Junko Tanaka; Kunio Harada; Akiko Shiratori; Yuzuru Shimazaki; Takahide Yokoi; Chihiro Uematsu; Yoshinobu Kohara
Source:
Analytical chemistry 2020 v.92 no.17 pp. 11705-11713
ISSN:
1520-6882
Subject:
DNA; analytical chemistry; color; dyes; fluorescence; genotyping; liquids; quantitative polymerase chain reaction; silicon; temperature
Abstract:
... Digital PCR (dPCR) is a promising method for performing liquid biopsies that quantifies nucleic acids more sensitively than real-time PCR. However, dPCR shows large fluctuations in the fluorescence intensity of droplets or wells due to insufficient PCR amplification in the small partitions, limiting the multiplexing capability of using the fluorescence intensity. In this study, we propose a measur ...
DOI:
10.1021/acs.analchem.0c01704

38. 100 Years of Chromosome Research in Rye, Secale L.

Author:
Rolf Schlegel
Source:
Plants 2022 v.11 no.13 pp. -
ISSN:
2223-7747
Subject:
DNA; barley; cytogenetic analysis; fish; fluorescence; genes; grasses; haploidy; meiosis; microscopy; mitosis; reciprocal translocation; rice; rye; sequence analysis; tetraploidy; trisomics; wheat
Abstract:
... Although microscopy and genetics were still in their infancy, there are cytological results produced a hundred years ago that are still relevant today. Since the 1920s, rye has been a subject of chromosome research. It started by plotting its mitotic and meiotic chromosomes to determine genome size. After controversial evidence, it became clear that the base number is n = 7. However, structural di ...
DOI:
10.3390/plants11131753

39. 102 Age-related differences in seminal parameters and expression of fertility marker genes in Marwari stallion

Author:
A. K. Chaudhary; G. N. Purohit; J. S. Mehta; S. K. Ravi; T. R. Talluri
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 158
ISSN:
1031-3613
Subject:
DNA; analysis of variance; artificial vagina; color; computer software; gels; gene expression regulation; mares; pH; plasma membrane; quantitative polymerase chain reaction; sperm concentration; sperm motility; spermatozoa; stallions; viability
Abstract:
... The objective of the present study was to observe age-related differences in seminal parameters and expression of fertility marker genes in Marwari stallions. Semen was collected from 9 Marwari stallions (6 ejaculates per stallion) of 3 different age groups (2 to 4 years, GI; 4 to 6 years, GII; and >6 years, GIII) twice a week using an artificial vagina by allowing the stallions to mount a mare. T ...
DOI:
10.1071/RDv33n2Ab102

40. 108 Fecal metabolite monitoring as a tool to assess sexual maturation in polar bears

Author:
E. Curry; M. A. Stoops; T. L. Roth
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 180
ISSN:
1031-3613
Subject:
DNA; Ursus maritimus; animal ovaries; enzyme immunoassays; excretion; feces; females; humans; juveniles; males; metabolites; monitoring; progeny; progesterone; pseudopregnancy; seasonal variation; sexual maturity; sires; t-test; testosterone
Abstract:
... Polar bears (Ursus maritimus) in managed care generally are paired for breeding starting around the age of 5 or 6 years; however, DNA analyses of the wild population indicate that males as young as 2 or 3 may sire offspring and females as young as 4 can produce cubs. There are no reports describing longitudinal reproductive hormone parameters in juvenile polar bears. The objective of the current s ...
DOI:
10.1071/RDv31n1Ab108

41. IL- 10R1 S138G loss-of-function polymorphism is associated with extrapulmonary tuberculosis risk development in Tunisia

Author:
Walid Ben-Selma; Yosra Ben-Abderrahmen; Jalel Boukadida; Hedi Harizi
Source:
Molecular biology reports 2012 v.39 no.1 pp. 51-56
ISSN:
0301-4851
Subject:
DNA; alleles; genotype; interleukin-10; loss-of-function mutation; molecular biology; odds ratio; polymerase chain reaction; risk; tuberculosis; Tunisia
Abstract:
... There is considerable evidence that host genetic factors are important in determining susceptibility to mycobacterial infections. More recently, functional genetic mutations affecting IL-10 receptor 1 (IL-10R1) were described. In this study, we investigated the relationship of IL-10R1 S138G loss-of-function polymorphism (A536G: rs3135932) with susceptibility to active tuberculosis (TB) in Tunisian ...
DOI:
10.1007/s11033-011-0709-2

42. 114 Effect of in vivo heat stress on DNA methylation and DNA hydroxymethylation of bovine oocytes

Author:
F. A. Diaz; E. J. Gutierrez; B. A. Foster; P. T. Hardin; K. R. Bondioli
Source:
Reproduction, fertility, and development 2019 v.31 no.1 pp. 183
ISSN:
1031-3613
Subject:
DNA; DNA methylation; antibodies; asses; beef cows; cold; epigenetics; fluorescence; fluorescent antibody technique; fluorescent dyes; germinal vesicle; goats; heat stress; immunoglobulin G; immunohistochemistry; least squares; metaphase; ovarian follicles; spring; summer; transcription (genetics); Louisiana
Abstract:
... Cattle under the effect of heat stress have reduced fertility, with negative effects on the oocyte observed at the morphological, biochemical, transcriptional and developmental levels. There are no studies evaluating the effect of heat stress on the epigenetic profile of bovine oocytes, which plays a fundamental role in the regulation of gamete development. The objective of this study was to evalu ...
DOI:
10.1071/RDv31n1Ab114

43. 114 Omega-3-enriched diet improves fertilization competence of cryopreserved sperm

Author:
D. Kalo; D. Reches; A. Komsky-Elbaz; U. Moallem; Y. Zeron; Z. Roth
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 184
ISSN:
1031-3613
Subject:
DNA; DNA fragmentation; Holstein; acrosome; blastocyst; bulls; cell viability; cryopreservation; dairy herds; diet; fatty acid composition; flow cytometry; freeze-thaw cycles; linseed oil; male fertility; membrane potential; mitochondrial membrane; oocytes; reactive oxygen species; saturated fatty acids; semen quality; slaughterhouses; sperm quality; straw
Abstract:
... Intensive reproductive management in dairy herds is mostly based on AI using high-merit bulls. Therefore, semen quality of bulls is of high importance. An association between semen quality and fatty acid content in feed has been suggested. Accordingly, the aim of this study was to examine the effect of omega-3 supplementation on sperm traits and fertilization competence. Fifteen Israeli Holstein b ...
DOI:
10.1071/RDv32n2Ab114

44. 119 Short spermatozoa-oocyte co-incubation improves outcomes of IVF in sheep

Author:
D. Anzalone; M. Czernik; L. Palazzese; Y. Ressaissi; P. Scapolo; P. Loi
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 186
ISSN:
1031-3613
Subject:
DNA; Pisum sativum; agglutinins; blastocyst; blood serum; differential staining; embryogenesis; estrus; humans; in vitro fertilization; isoproterenols; oocytes; polyspermy; propidium; reproductive performance; risk; sheep; spermatozoa; zona pellucida
Abstract:
... The assisted reproductive technique IVF is routinely applied in humans and large animals, both to boost reproductive performance and also for basic research. Despite its value, IVF has seen very little progress in the last two decades and relies on established paradigms, such as overnight sperm-egg co-incubation. However, the long exposure of oocytes to spermatozoa in a dish increases the risk of ...
DOI:
10.1071/RDv32n2Ab119

45. A 12-deoxywithastramonolide-rich somaclonal variant in Withania somnifera (L.) Dunal—molecular cytogenetic analysis and significance as a chemotypic resource

Author:
Satiander Rana; Niha Dhar; Wajid W. Bhat; Sumeer Razdan; Shabnam Khan; Rekha S. Dhar; Prabhu Dutt; Surrinder K. Lattoo
Source:
In vitro cellular & developmental biology 2012 v.48 no.5 pp. 546-554
ISSN:
1054-5476
Subject:
DNA; Panax; Withania somnifera; chromosome number; chromosomes; clones; cytogenetic analysis; explants; genetic polymorphism; high performance liquid chromatography; indole butyric acid; kinetin; leaves; medicinal plants; meiosis; mitosis; organogenesis; phenotype; random amplified polymorphic DNA technique; secondary metabolites; somaclonal variation
Abstract:
... Withania somnifera, commonly known as ashwagandha or Indian ginseng, is a valuable medicinal plant, synthesizing a wide array of pharmacologically active secondary metabolites known as withanolides. In this study, we investigated variation among 54 regenerated plants attained through indirect organogenesis from leaf explants. Organogenic calli were induced on Murashige and Skoog medium containing ...
DOI:
10.1007/s11627-012-9458-8

46. A 126 bp fragment of a plant histone gene promoter confers preferential expression in meristems of transgenic Arabidopsis

Author:
Atanassova, R.; Chaubet, N.; Gigot, C.
Source:
plant journal 1992 v.2 no.3 pp. 291-300
ISSN:
0960-7412
Subject:
Arabidopsis; genetic transformation; Agrobacterium; transgenic plants; histones; gene expression; beta-glucuronidase; enzyme activity; meristems; messenger RNA; chromosome mapping; histochemistry; promoter regions
DOI:
10.1111/j.1365-313X.1992.00291.x
PubMed:
1303796

47. 12S rRNA mitochondrial gene as marker to trace Sicilian mono-species dairy products

Author:
Lina Tortorici; Rosalia Di Gerlando; Marco Tolone; Salvatore Mastrangelo; Maria Teresa Sardina
Source:
Livestock science 2016 v.193 pp. 39-44
ISSN:
1871-1413
Subject:
Capra hircus; DNA; adulterated products; agarose; blood; breeds; cheeses; cows; electrophoresis; ewe milk; ewes; gels; genes; goat milk; goats; milk; mitochondria; ribosomal RNA; traceability
Abstract:
... For a rapid, specific and sensitive identification of cows', ewes' and goats' milk in mono-species Sicilian dairy products, species-specific duplex-PCR protocol was applied. DNA samples from blood and experimental cheeses of Sicilian autochthonous breeds were extracted to amplify the 12S rRNA (and part of 16S rRNA in case of Ovis aries) mitochondrial species-specific gene fragment. The use of spec ...
DOI:
10.1016/j.livsci.2016.09.015

48. IL- 13 downregulates PPAR-γ/heme oxygenase-1 via ER stress-stimulated calpain activation: aggravation of activated microglia death

Author:
Liu, Shing Hwa; Yang, Cheng Ning; Pan, Hung Chuan; Sung, Yen Jen; Liao, Ko Kaung; Chen, Wen Bao; Lin, Wen Zheng; Sheu, Meei Ling
Source:
Cellular and molecular life sciences 2010 v.67 no.9 pp. 1465-1476
ISSN:
1420-682X
Subject:
DNA; agonists; antagonists; apoptosis; calpain; caspase-12; death; endoplasmic reticulum; heme oxygenase (biliverdin-producing); interleukin-10; interleukin-13; lipopolysaccharides; neuroglia; protein synthesis; small interfering RNA; transcriptional activation; transfection
Abstract:
... Interleukin 13 (IL-13) has been shown to induce the death of activated microglia. We observed that IL-13, but not IL-4 or IL-10, significantly enhanced endoplasmic reticulum (ER) stress induction, apoptosis and death in microglia activated by lipopolysaccharide (LPS). IL-13 enhanced ER stress-regulated calpain activation and calpain-II expression in LPS-activated microglia. Calpain-II siRNA effect ...
DOI:
10.1007/s00018-009-0255-4

49. 13-Acetoxysarcocrassolide Exhibits Cytotoxic Activity against Oral Cancer Cells through the Interruption of the Keap1/Nrf2/p62/SQSTM1 Pathway: The Need to Move Beyond Classical Concepts

Author:
Yi-Chang Liu; Bo-Rong Peng; Kai-Cheng Hsu; Mohamed El-Shazly; Shou-Ping Shih; Tony Eight Lin; Fu-Wen Kuo; Yi-Cheng Chou; Hung-Yu Lin; Mei-Chin Lu
Source:
Marine drugs 2020 v.18 no.8 pp. -
ISSN:
1660-3397
Subject:
DNA; Western blotting; acetylcysteine; animal models; antineoplastic activity; antineoplastic agents; antioxidant enzymes; apoptosis; cell viability; corals; cytotoxicity; enzyme activity; flow cytometry; fluorescent antibody technique; immunostimulants; mechanism of action; membrane potential; mitochondrial membrane; mouth neoplasms; neoplasm cells; precipitin tests; proteins; reactive oxygen species; small interfering RNA; toxicity testing; transfection; xenotransplantation
Abstract:
... 13-Acetoxysarcocrassolide (13-AC), a marine cytotoxic product isolated from the alcyonacean coral Lobophytum crassum, exhibited potent antitumor and immunostimulant effects as reported in previous studies. However, the 13-AC antitumor mechanism of action against oral cancer cells remains unclear. The activity of 13-AC against Ca9-22 cancer cells was determined using MTT assay, flow cytometric anal ...
DOI:
10.3390/md18080382

50. 135 Strategies for transfection of bovine mesenchymal stem cells with pBC1-anti-CD3 vector

Author:
F. B. Duarte; S. N. Báo; M. Brígido; J. M. Araújo; E. d. O. Melo; C. F. Martins
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 194
ISSN:
1031-3613
Subject:
DNA; Southern blotting; T-lymphocytes; adipocytes; adipose tissue; air; autoimmune diseases; bioreactors; carbon dioxide; cattle; culture dishes; fetal bovine serum; fibroblasts; flow cytometry; fluorescence; fluorescence microscopy; funding; genetically modified organisms; human diseases; humidity; mesenchymal stromal cells; monoclonal antibodies; necrosis; neomycin; polymerase chain reaction; transfection
Abstract:
... Cells from different origins behave differently regarding the incorporation of exogenous genetic material and the formation of transgenic cells. In this context, the objective of this study was to verify the potential of transfection of bovine mesenchymal stem cells from Wharton's jelly and adipose tissue, comparing two transfection protocols, using Lipofectamine LTX and Plus or Xfect reagents, wi ...
DOI:
10.1071/RDv32n2Ab135