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1. 1-Methylcyclopropene (1-MCP) treatment differentially mediated expression of vacuolar processing enzyme (VPE) genes and delayed programmed cell death (PCD) during ripening and senescence of apple fruit

Author:
Jingyi Lv; Yingzhi Zhang; Mingyu Sun; Jingxin Chen; Yonghong Ge; Jianrong Li
Source:
Scientia horticulturae 2023 v.307 pp. 111489
ISSN:
0304-4238
Subject:
1-methylcyclopropene; DNA; Malus domestica; apples; climacteric fruits; electrical conductivity; enzymes; ethylene; firmness; malondialdehyde; programmed cell death; reactive oxygen species; transmission electron microscopy; vacuoles
Abstract:
... The main purpose of this research was to evaluate the impacts of 1-methylcyclopropene (1-MCP) treatment on vacuolar processing enzyme (VPE) genes and programmed cell death (PCD) during ripening and senescence of apple fruit (Malus × domestica Borkh.). The ethylene antagonists 1-MCP (1 μL L⁻¹) was applied to apples after harvest. Both treated and control fruit were stored at 20 ± 2°C. The data show ...
DOI:
10.1016/j.scienta.2022.111489

2. Anchoring red blood cell with tetrahedral DNA nanostructure: Electrochemical biosensor for the sensitive signage of circulating tumor DNA

Author:
Xinyue Zha; Weiwei Qin; Jiadi Chen; Mengting Chen; Qiyue Zhang; Kaiyu He; Yingju Liu; Weipeng Liu
Source:
Talanta 2023 v.251 pp. 123793
ISSN:
0039-9140
Subject:
DNA; biocompatibility; biosensors; blood serum; detection limit; erythrocytes; methylene blue; neoplasms; rapid methods
Abstract:
... Circulating tumor cells (CTCs), as a type of tumor, have attracted wide attention because of their characteristics of shedding from the primary tumor and spreading to other tissues and organs through peripheral blood. The circulating tumor DNA (ctDNA), the DNA released by CTCs and other tumor cells into the peripheral blood, was considered as a promising detection substance for clinical applicatio ...
DOI:
10.1016/j.talanta.2022.123793

3. Capsalid monogeneans of fishes from the Seto Inland Sea, Japan: Description of Benedenia kobudai n. sp. parasitic on Semicossyphus reticulatus (Perciformes: Labridae)

Author:
Masato Nitta
Source:
Parasitology international 2023 v.92 pp. 102677
ISSN:
1383-5769
Subject:
Capsalidae; DNA; Japan; Semicossyphus; aquaculture; biodiversity; fish; fisheries; host range; new species; parasitology; penis; phylogeny
Abstract:
... The Seto Inland Sea, the largest inland sea in Japan, is a rich fishery with high biodiversity and productivity. Monogeneans have been studied for more than 120 years, and 58 nominal species have been recorded in the Seto inland Sea. This study provided DNA information on five species of Benedenia sensu lato (Capsalidae) from marine fishes from the sea, and one of them, Benedenia kobudai n. sp., i ...
DOI:
10.1016/j.parint.2022.102677

4. Cascaded molecular logic gates using antibiotics as inputs based on exonuclease III and DNAzyme

Author:
Fang Deng; Jiafeng Pan; Zhi Liu; Junhua Chen
Source:
Talanta 2023 v.252 pp. 123832
ISSN:
0039-9140
Subject:
DNA; analytical methods; catalytic activity; chloramphenicol; fluorescence; kanamycin; nucleic acid hybridization; oxytetracycline
Abstract:
... We successfully constructed several cascaded molecular logic gates (2INHIBIT-2AND, 2AND-2OR, and 2OR-2INHIBIT) using three different antibiotics as the inputs. In the presence of kanamycin (KAN), chloramphenicol (CHL), or oxytetracycline (OXY), the aptamer-antibiotic recognition will release the trigger DNA to active the hairpin DNA hybridization. Exonuclease III (Exo III)-mediated catalysis react ...
DOI:
10.1016/j.talanta.2022.123832

5. Characterization and functional analysis of chicken dsRNA binding protein hnRNPU

Author:
Xifeng Hu; Xiangdong Wu; Zhen Ding; Zheng Chen; Huansheng Wu
Source:
Developmental and comparative immunology 2023 v.138 pp. 104521
ISSN:
0145-305X
Subject:
DNA; RNA; Vesiculovirus; chickens; cytosol; genomics; humans; immunology; nuclear matrix; ribonucleoproteins
Abstract:
... In mammals, heterogeneous ribonucleoprotein U (hnRNPU), also named as nuclear matrix protein-nuclear scaffold attachment factor (SAFA), was originally identified as a DNA/RNA interactor protein. It has been reported that human hnRNPU facilitates IFN-β generation after vesicular stomatitis virus (VSV) infection. Nevertheless, the role of chicken hnRNPU (chhnRNPU) in IFN-β regulation as well as in i ...
DOI:
10.1016/j.dci.2022.104521

6. DNA-based surrogates for the validation of microbial inactivation using cold atmospheric pressure plasma and plasma-activated water processing

Author:
Hemiao Cui; Qingyang Wang; Rewa Rai; Deepti Salvi; Nitin Nitin
Source:
Journal of food engineering 2023 v.339 pp. 111267
ISSN:
0260-8774
Subject:
DNA; Fourier transform infrared spectroscopy; algorithms; atmospheric pressure; biofilm; cold; decision support systems; decontamination
Abstract:
... Cold atmospheric pressure plasma (CAPP) and plasma-activated water (PAW) are emerging nonthermal technologies. Due to the complexity of plasma-generated species as a function of plasma generation conditions, there is a need to develop process validation. This study aims to evaluate plasma interactions with DNA-based surrogates using infrared spectroscopy and gradient boosting decision tree, a mach ...
DOI:
10.1016/j.jfoodeng.2022.111267
CHORUS:
10.1016/j.jfoodeng.2022.111267

7. Dietary fiber konjac glucomannan exerts an antidiabetic effect via inhibiting lipid absorption and regulation of PPAR-γ and gut microbiome

Author:
Muthukumaran Jayachandran; Sunil Christudas; Xuchu Zheng; Baojun Xu
Source:
Food chemistry 2023 v.403 pp. 134336
ISSN:
0308-8146
Subject:
absorption; blood lipids; body weight; dietary fiber; drugs; fibrosis; food chemistry; glycemic effect; high fat diet; hyperlipidemia; immunohistochemistry; insulin; intestinal microorganisms; konjac mannan; lipid composition; lipid metabolism; lipids; liver; microbiome; noninsulin-dependent diabetes mellitus; protein synthesis; streptozotocin
Abstract:
... Konjac glucomannan’s influence on the regulation of diabetes mellitus, hyperlipidemia, and gut microbial flora was evaluated in this study. In addition, a high-fat diet and streptozotocin were used to induce type 2 diabetes mellitus in rats. At the end of the study, we analyzed various parameters such as body weight, plasma lipid profile, insulin levels by immunohistochemistry, degree of fibrosis ...
DOI:
10.1016/j.foodchem.2022.134336

8. Dietary tryptophan requirement of fingerling Channa punctatus (Bloch) based on growth, hematological parameters, intestinal enzymes, non-specific immune response, and antioxidant capacity

Author:
Yusra Sharf; Mukhtar A. Khan
Source:
Aquaculture 2023 v.562 pp. 738745
ISSN:
0044-8486
Subject:
Channa punctata; DNA; RNA; alanine transaminase; antioxidant activity; aquaculture; aspartate transaminase; casein; catalase; erythrocyte sedimentation rate; fingerlings; gelatin; glutathione peroxidase; hematocrit; hemoglobin; immune response; intestines; lysozyme; malondialdehyde; regression analysis; satiety; specific growth rate; superoxide dismutase; tryptophan; weight gain
Abstract:
... In this investigation, the tryptophan requirement of C. punctatus fingerling (5.45 ± 0.07 g) was determined by feeding seven isoproteic (450 g kg⁻¹ CP) and isoenergetic (14.7 kJ g⁻¹ DE) diets with graded levels of tryptophan 1, 2, 3, 4, 5, 6, and 10 g kg⁻¹, respectively to randomly stocked groups in triplicate at 8:00, 12:30 and 17:30 h near to satiety for 12 weeks. Tryptophan contributed by casei ...
DOI:
10.1016/j.aquaculture.2022.738745

9. Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement

Author:
Cuiying Lin; Qingqing Huang; Mengjian Tian; Fang Luo; Jian Wang; Bin Qiu; Shuofei Yang; Zhenyu Lin
Source:
Talanta 2023 v.251 pp. 123748
ISSN:
0039-9140
Subject:
DNA; adenine; biosensors; blood serum; detection limit; electrochemiluminescence; electrodes; ferrocenes; indium; methylation; methyltransferases; restriction endonucleases; tin
Abstract:
... In this work, an electrochemiluminescence (ECL) biosensor with dual signal enhancement was constructed and used for DNA adenine methylation methyltransferase (Dam MTase) detection. At present of Dam MTase, restriction endonuclease (DPnI) cleaves hairpin DNA (HP) and releases the HP stem end as a single strand that can activate CRISPR/Cas12a trans-cleavage activity. Assisted by trans-cleavage, the ...
DOI:
10.1016/j.talanta.2022.123748

10. Genome–wide association study and genomic prediction of growth traits in bighead catfish (Clarias macrocephalus Günther, 1864)

Author:
Prapaiphan Chaivichoo; Sila Sukhavachana; Rabuesak Khumthong; Prapansak Srisapoome; Satid Chatchaiphan; Uthairat Na-Nakorn
Source:
Aquaculture 2023 v.562 pp. 738748
ISSN:
0044-8486
Subject:
Clarias macrocephalus; DNA; aquaculture; body weight; catfish; cost effectiveness; genes; genetic improvement; genetic variance; genome-wide association study; genomics; genotyping; heritability; marker-assisted selection; parentage; phenotype; prediction; technology; variance; zinc finger motif
Abstract:
... Next-generation sequencing (NGS) enables cost-effective exploration of genome-wide single nucleotide polymorphisms (SNPs). This new technology has made enormous contributions in various fields including genetic improvement. This study was conducted to identify SNPs that associated with growth traits (body weight, standard length, and total length) of bighead catfish (Clarias macrocephalus Günther, ...
DOI:
10.1016/j.aquaculture.2022.738748

11. High-performance strategy for the construction of electrochemical biosensor for simultaneous detection of miRNA-141 and miRNA-21 as lung cancer biomarkers

Author:
Ali Khodadoust; Navid Nasirizadeh; Seyed Morteza Seyfati; Ramezan Ali Taheri; Mostafa Ghanei; Hasan Bagheri
Source:
Talanta 2023 v.252 pp. 123863
ISSN:
0039-9140
Subject:
DNA; DNA probes; biomarkers; biosensors; glassy carbon electrode; hybridization; lung neoplasms; microRNA; signal-to-noise ratio
Abstract:
... In this study, the dual signal-labeled hairpin-structured DNA (dhDNA)-based probes have been developed to construct a novel nano-biosensor. This one hairpin-structured probe consists of a thiolated methylene blue-labeled hairpin capture probe (MB-HCP) as an inner reference probe and a ferrocene-modified anti-miRNA-21 DNA probe (Fc-AP-21). This novel integrated structure of MB-HCP and Fc-AP-21 was ...
DOI:
10.1016/j.talanta.2022.123863

12. Improved sperm DNA integrity and altered fat metabolism and intestinal morphology in Nile tilapia (Oreochromis niloticus) males fed rations with purified nucleotide

Author:
Robie Allan Bombardelli; Juliano Kelvin dos Santos Henriques; Marta Margarete Cestari; Antônio Ernesto Meister Luz Marques; Taís Voelkl Chagas; Fabio Meurer
Source:
Aquaculture 2023 v.562 pp. 738682
ISSN:
0044-8486
Subject:
DNA; Oreochromis niloticus; aquaculture; blood glucose; chromatin; diet; digestible energy; digestible protein; enterocytes; fish; gonadosomatic index; hepatosomatic index; high density lipoprotein; lipid content; lipid metabolism; liver; morphometry; muscles; nucleotides; testes; triacylglycerols; visceral fat; viscosity
Abstract:
... In this study, we assessed the health, growth, sperm characteristics, and intestinal, liver, and testicular morphology of Nile tilapia (Oreochromis niloticus) males fed rations containing purified nucleotides (NT). A total of 420 females (286 ± 7.15 g) and 140 males (536 ± 13.4 g) were used. Fish were fed on extruded ration (280 g digestible protein kg⁻¹; 11.72 M joule (MJ) of digestible energy kg ...
DOI:
10.1016/j.aquaculture.2022.738682

13. Insights into a novel nitrogen removal process based on simultaneous anammox and denitrification (SAD) following nitritation with in-situ NOB elimination

Author:
Jing Chen; Jia Zeng; Yiran He; Shiquan Sun; Haipeng Wu; Yaoyu Zhou; Zhenguo Chen; Jianhui Wang; Hong Chen
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 160-170
ISSN:
1001-0742
Subject:
Comamonadaceae; DNA; anaerobic ammonium oxidation; batch systems; denitrification; nitrogen; total nitrogen; wastewater; wastewater treatment; zeolites; China
Abstract:
... Simultaneous anammox and denitrification (SAD) is an efficient approach to treat wastewater having a low C/N ratio; however, few studies have investigated a combination of SAD and partial nitritation (PN). In this study, a lab-scale up-flow blanket filter (UBF) and zeolite sequence batch reactor (ZSBR) were continuously operated to implement SAD and PN advantages, respectively. The UBF achieved a ...
DOI:
10.1016/j.jes.2022.01.019

14. Integrated multi-omic analyses on yellow Flammulina filiformis cultivar reveal postharvest oxidative damage responses

Author:
Yu Fu; Hao Tan; Bo Wang; Weihong Peng; Qun Sun; Yang Yu
Source:
Postharvest biology and technology 2023 v.195 pp. 112111
ISSN:
0925-5214
Subject:
DNA; Flammulina; biosynthesis; catechol oxidase; cell senescence; cultivars; genome; laccase; melanin; mushrooms; oxidative phosphorylation; oxygen; proteolysis; proteome; proteomics; technology; transcriptome; transcriptomics; tyrosine
Abstract:
... Yellow cultivars of Flammulina filiformis are prone to browning, which causes significant yearly loss. This study investigated the critical factors involved and revealed that oxygen plays an essential role in the browning process. Integrated multi-omic analyses, including genome sequencing, transcriptome profiling, and 4D label-free proteome quantification, were incorporated to dissect essential g ...
DOI:
10.1016/j.postharvbio.2022.112111

15. Interaction of Polycyclic Aromatic Hydrocarbon compounds in fish primary hepatocytes: From molecular mechanisms to genotoxic effects

Author:
Isabella Bramatti; Beatriz Matos; Neusa Figueiredo; Pedro Pousão-Ferreira; Vasco Branco; Marta Martins
Source:
Science of the total environment 2023 v.855 pp. 158783
ISSN:
0048-9697
Subject:
DNA; DNA damage; Sparus aurata; Western blotting; carcinogenicity; cell viability; chemical structure; comet assay; environment; environmental assessment; fish; genes; genotoxicity; hepatocytes; metabolites; mutagens; phenanthrenes; protein synthesis; risk assessment
Abstract:
... Polycyclic Aromatic Hydrocarbons (PAHs) are persistent pollutants normally found in the environment as complex mixtures. Although several individual PAHs are classified as mutagenic and carcinogenic pollutants, the interaction effects between compounds in a mixture may trigger different toxicological mechanisms and, consequently, yield different effects to organisms which are not accounted for in ...
DOI:
10.1016/j.scitotenv.2022.158783

16. Investigation of the latency of Cyprinid herpesvirus 2 in apparently healthy farmed gibel carp, Carassius auratus gibelio

Author:
Chang Wei; Chaonan Xu; Yuyu Sun; Jinzhou Li; Motohiko Sano; Qiang Li
Source:
Aquaculture 2023 v.562 pp. 738854
ISSN:
0044-8486
Subject:
Carassius auratus; Carassius gibelio; Cyprinid herpesvirus 2; DNA; aquaculture; carrier state; gene expression; genes; goldfish; kidneys; necrosis; pathogens; transcription (genetics); viruses
Abstract:
... Cyprinid herpesvirus 2 (CyHV-2) is a highly contagious pathogen that causes herpesviral haematopoietic necrosis (HVHN) disease to goldfish, Carassius auratus and gibel carp, C. auratus gibelio. In this study, the virus persistence or latency in apparently healthy farmed gibel carp and reactivation in vitro were investigated. All of the freshly dissected kidney tissues of 42 individuals of asymptom ...
DOI:
10.1016/j.aquaculture.2022.738854

17. Label free flexible electrochemical DNA biosensor for selective detection of Shigella flexneri in real food samples

Author:
M.R. Ali; M.S. Bacchu; S. Das; S. Akter; M.M. Rahman; M.Aly Saad Aly; M.Z.H. Khan
Source:
Talanta 2023 v.253 pp. 123909
ISSN:
0039-9140
Subject:
DNA; Shigella flexneri; biosensors; detection limit; electrochemistry; electrodes; foodborne bacterial pathogens; indium tin oxide; melamine; sodium
Abstract:
... An effective tool for early-stage selective detection of the foodborne bacterial pathogen Shigella flexneri (S. flexneri) is essential for diagnosing infectious diseases and controlling outbreaks. Here, a label-free electrochemical DNA biosensor for monitoring S. flexneri is developed. To fabricate the biosensor, detection probe (capture probe) is immobilized on the surface of poly melamine (P-Mel ...
DOI:
10.1016/j.talanta.2022.123909

18. Methylome and transcriptome analysis of alters leaf phenotype with autotetraploid in grape

Author:
Xiaodong Xiang; Yukun Gao; Jianghui Cui; Genzeng Ren; Congpei Yin; Jinhua Chang
Source:
Scientia horticulturae 2023 v.307 pp. 111534
ISSN:
0304-4238
Subject:
5-methylcytosine; DNA; Vitis vinifera; anthocyanins; antioxidant activity; autotetraploidy; biosynthesis; bisulfites; cell walls; diploidy; epigenetics; gene expression; gene expression regulation; genomics; grapes; homeostasis; introns; leaf thickness; leaves; lipid metabolism; methylation; phenotype; photosynthesis; plant hormones; secondary metabolites; transcriptomics
Abstract:
... The doubling of the plant genome causes obvious morphological and physiological changes, and DNA 5-methylcytosine (5mCs) modification is a key epigenetic component that helps neopolyploids overcome challenges and acquire advantages. In the study of autotetraploid grape, we found that leaf thickness, photosynthetic and antioxidant capacity increased and anthocyanin coloring decreased in autotetrapl ...
DOI:
10.1016/j.scienta.2022.111534

19. Nano-gold mediated aptasensor for colorimetric monitoring of acrylamide: Smartphone readout strategy for on-site food control

Author:
Zahra Khoshbin; Marzieh Moeenfard; Khalil Abnous; Seyed Mohammad Taghdisi
Source:
Food chemistry 2023 v.399 pp. 133983
ISSN:
0308-8146
Subject:
DNA; acrylamides; aptasensors; breads; color; colorimetry; food chemistry; food safety; mobile telephones; nanogold
Abstract:
... A simple aptasensor is embedded in the internal surface of a micropipette tip as the aptasensor substrate for the label-free monitoring of acrylamide. The aptasensor is based on the formation of the triple-helix molecular switch (THMS) structure of the DNA strands that induces the salt-enforced aggregation of gold nanoparticles (AuNPs). A smartphone imaging readout-based strategy is applied to qua ...
DOI:
10.1016/j.foodchem.2022.133983

20. Nondestructive and rapid method for sex identification of the tropical sea cucumber Holothuria scabra by anal swab sampling

Author:
Zixuan E; Chuhang Cheng; Feifei Wu; Chunhua Ren; Rong Chen; Yingzhu Rao; Bo Ma; Xiao Jiang; Peng Luo; Xiaomin Li; Xin Zhang; Fajun Jiang; Chaoqun Hu; Ting Chen
Source:
Aquaculture 2023 v.562 pp. 738749
ISSN:
0044-8486
Subject:
DNA; Holothuria scabra; aquaculture; dimorphism; females; genomics; loop-mediated isothermal amplification; males; phenotype; rapid methods; reproduction; South East Asia
Abstract:
... As one of the most economically significant sea cucumber species, Holothuria scabra are naturally distributed in the Indo-West Pacific region and are widely cultural in Southeast Asia. The appearances of female and male H. scabra have no obvious phenotypic dimorphism. Thus, identification of sex-specific markers and establishment of sex detection methods are beneficial to artificially induced repr ...
DOI:
10.1016/j.aquaculture.2022.738749

21. Phenolics from noni (Morinda citrifolia L.) fruit alleviate obesity in high fat diet-fed mice via modulating the gut microbiota and mitigating intestinal damage

Author:
Ruimin Wang; Lu Wang; Shengwei Wang; Juntao Wang; Congyan Su; Lin Zhang; Congfa Li; Sixin Liu
Source:
Food chemistry 2023 v.402 pp. 134232
ISSN:
0308-8146
Subject:
Morinda citrifolia; anti-obesity effects; dysbiosis; food chemistry; fruits; high fat diet; inflammation; intestinal microorganisms; intestines; lipopolysaccharides; liver; obesity; occludins; oxidative stress; phenolic compounds; protein synthesis; short chain fatty acids
Abstract:
... Noni fruit has certain anti-obesity effect. However, the bioactive ingredients in noni fruit that contribute to anti-obesity activity as well as the relation between its anti-obesity activity and gut microbiota remain unclear. In this study, obese mice induced by high-fat diet (HFD) and were intervened with noni fruit phenolic extract (NFE) for 10 weeks. The results showed NFE supplementation decr ...
DOI:
10.1016/j.foodchem.2022.134232

22. Phylogeny, character evolution, and biogeography of the fern genus Bolbitis (Dryopteridaceae)

Author:
Li-Yun Nie; Liang Zhang; Zhen-Long Liang; Rossarin Pollawatn; Yue-Hong Yan; Ngan Thi Lu; Ralf Knapp; Xia Wan; Daniele Cicuzza; Xin-Xin Chen; Hong-Feng Chen; Ai-Hua Wang; Yu-Jie Liao; Fa-Guo Wang; Li-Bing Zhang
Source:
Molecular phylogenetics and evolution 2023 v.178 pp. 107633
ISSN:
1055-7903
Subject:
Bolbitis; DNA; biogeography; ferns and fern allies; geographical distribution; monophyly; paraphyly; polyphyly; Africa; Asia
Abstract:
... Bolbitis is a pantropical fern genus of Dryopteridaceae with ca. 80 species mainly in tropical Asia. Earlier studies confirmed the monophyly of Bolbitis when Mickelia is excluded and identified three major clades in Bolbitis. However, earlier studies are based on relatively small sampling and the majority of Asian species are not sampled. In this study, DNA sequences of three plastid markers of 16 ...
DOI:
10.1016/j.ympev.2022.107633

23. Real-time PCR, and Recombinase Polymerase Amplification combined with SYBR Green I for naked-eye detection, along with Propidium Monoazide (PMA) for the detection of viable patulin-producing fungi in apples and by-products

Author:
Foteini Roumani; Jorge Barros-Velázquez; Alejandro Garrido-Maestu; Marta Prado
Source:
Food control 2023 v.144 pp. 109347
ISSN:
0956-7135
Subject:
DNA; Penicillium; biochemical pathways; food safety; genes; patulin; propidium; quantitative polymerase chain reaction; recombinase polymerase amplification; toxicity
Abstract:
... Nowadays, the application of rapid molecular-based methods such as PCR/qPCR and isothermal techniques like Recombinase Polymerase Amplification (RPA), has increased in order to overcome some of the drawbacks of traditional culture-based methods due to their high sensitivity and specificity. One of the main limitations of these techniques is their inability to differentiate between live and dead mi ...
DOI:
10.1016/j.foodcont.2022.109347

24. Synergistic disinfection of aerosolized bacteria and bacteriophage by far-UVC (222-nm) and negative air ions

Author:
Y.H. Lu; H. Wu; H.H. Zhang; W.S. Li; A.C.K. Lai
Source:
Journal of hazardous materials 2023 v.441 pp. 129876
ISSN:
0304-3894
Subject:
DNA; Escherichia coli; Staphylococcus epidermidis; air; bacteriophages; bioaerosols; disinfection; ultraviolet radiation
Abstract:
... Air ionizers and 222-nm krypton-chlorine (KrCl) excilamp have proven to be effective disinfection apparatus for bacteria and viruses with limited health risks. We determined inactivation efficiencies by operating them individually and in combined modules. Gram-positive and gram-negative bacteria, non-enveloped dsDNA virus, and enveloped dsRNA virus were examined in a designed air disinfection syst ...
DOI:
10.1016/j.jhazmat.2022.129876

25. TMT-based quantitative proteomic and physiological analyses on lotus plumule of artificially aged seed in long-living sacred lotus Nelumbo nucifera

Author:
Dongli He; Mengmeng Cai; Meihui Liu; Pingfang Yang
Source:
Journal of proteomics 2023 v.270 pp. 104736
ISSN:
1874-3919
Subject:
DNA; Nelumbo nucifera; RNA; cell membranes; germination; histology; longevity; nutritive value; photosynthesis; physiological state; plumule; protein composition; proteome; proteomics; seed longevity; seed quality; starch; sucrose; vigor
Abstract:
... Seed longevity is important for the maintenance of seed nutritional quality, vigor, and germination potential during storage. Sacred lotus is known as one of the longest living seeds in the world and their ability to maintain longevity has been widely investigated. In this study, a suitable controlled deterioration treatment (CDT) method was first established to evaluate the vigor loss of lotus pl ...
DOI:
10.1016/j.jprot.2022.104736

26. Temporal profiling resolves the drivers of microbial nitrogen cycling variability in coastal sediments

Author:
Alexis J. Marshall; Lori Phillips; Andrew Longmore; Helen L. Hayden; Karla B. Heidelberg; Caixian Tang; Pauline Mele
Source:
Science of the total environment 2023 v.856 pp. 159057
ISSN:
0048-9697
Subject:
Archaea; DNA; RNA; ammonium; anaerobic ammonium oxidation; coastal zone management; detection limit; environment; genes; microbial activity; microbial nitrogen; nitrates; nitrites; nitrogen fixation; oxidation; phylogeny; seasonal variation; sediments; Australia
Abstract:
... Here we describe the potential for sediment microbial nitrogen-cycling gene (DNA) and activity (RNA) abundances to spatially resolve coastal areas impacted by seasonal variability in external nutrient inputs. Three sites were chosen within a nitrogen-limited embayment, Port Phillip Bay (PPB), Australia that reflect variability in both proximity to external nutrient inputs and the dominant form of ...
DOI:
10.1016/j.scitotenv.2022.159057

27. Understanding the influence of the bioaerosol source on the distribution of airborne bacteria in hospital indoor air

Author:
Víctor Pertegal; Engracia Lacasa; Pablo Cañizares; Manuel A. Rodrigo; Cristina Sáez
Source:
Environmental research 2023 v.216 pp. 114458
ISSN:
0013-9351
Subject:
DNA; Escherichia coli; World Health Organization; air; air flow; bioaerosols; cell membranes; hospitals; models; osmotic pressure; relative humidity; research; saliva; urine; wastewater
Abstract:
... The composition and concentration of airborne microorganisms in hospital indoor air has been reported to contain airborne bacteria and fungi concentrations ranged 10¹–10³ CFU/m³ in inpatients facilities which mostly exceed recommendations from the World Health Organization (WHO). In this work, a deeper knowledge of the performance of airborne microorganisms would allow improving the designs of the ...
DOI:
10.1016/j.envres.2022.114458

28. Unraveling the mechanism of the synergistic antimicrobial effect of cineole and carvacrol on Escherichia coli O157:H7 inhibition and its application on fresh-cut cucumbers

Author:
Keren Agyekumwaa Addo; Hui Li; Yigang Yu; Xinglong Xiao
Source:
Food control 2023 v.144 pp. 109339
ISSN:
0956-7135
Subject:
DNA; Escherichia coli O157; Shiga toxin; antimicrobial properties; bacteria; biofilm; carvacrol; cineole; exopolysaccharides; flagellum; food safety; fresh produce; genes; intimin; mechanism of action; quorum sensing; sanitizers; transcription (genetics); virulence
Abstract:
... The objective of this study was to evaluate the mechanism of the synergistic antimicrobial effect of cineole (CN) and carvacrol (CR) on E. coli and its application on fresh-cut cucumbers. Herein, the CN/CR synergy could effectively inhibit E. coli by destroying its bacterial cell structure and causing the leakage of intracellular organic matters. For the first time, CN/CR synergy interaction on E. ...
DOI:
10.1016/j.foodcont.2022.109339

29. An autonomous driven DNA walker-based electrochemical aptasensor for on-site detection of Ochratoxin A

Author:
Tongfu Huang; Mei Wang; Nian Hong; Hanfeng Cui; Qiqi Fan; Guobing Wei; Longshua Qin; Jing Zhang; Hao Fan
Source:
Talanta 2023 v.252 pp. 123785
ISSN:
0039-9140
Subject:
DNA; aptasensors; coordination polymers; electrochemistry; ochratoxin A
Abstract:
... Herein, an autonomous driven DNA walker-based aptasensor is proposed for the detection of Ochratoxin A (OTA). A nanoscale metal-organic framework (MOF) is synthesized and used to load Mn²⁺, a cofactor for DNAzyme. The DNA walker and Mn²⁺@MOF are assembled on the Au surface, integrating the DNA walker and metal auxiliary ions. The proposed sensor avoids the addition of metal auxiliary ions of DNAzy ...
DOI:
10.1016/j.talanta.2022.123785

30. A biodegradable and cofactor self-sufficient aptazyme nanoprobe for amplified imaging of low-abundance protein in living cells

Author:
Jiayao Xu; Lifang Yao; Xiaohong Zhong; Kun Hu; Shulin Zhao; Yong Huang
Source:
Talanta 2023 v.253 pp. 123983
ISSN:
0039-9140
Subject:
DNA; biodegradability; detection limit; flow cytometry; fluorescence; fluorescent dyes; nanorods; neoplasms; oligonucleotides
Abstract:
... Despite the progress on the analysis of proteins either in vitro or in vivo, detection and imaging of low-abundance proteins in living cells still remains challenging. Herein, a novel biodegradable and cofactor self-sufficient DNAzyme nanoprobe has been deve-loped for catalytic imaging of protein in living cells with signal amplification capacity. This DNAzyme nanoprobe is constructed by assemblin ...
DOI:
10.1016/j.talanta.2022.123983

31. An electrochemical biosensor for the highly sensitive detection of Staphylococcus aureus based on SRCA-CRISPR/Cas12a

Author:
Luqi Huang; Ning Yuan; Wei Guo; Yunzhe Zhang; Wei Zhang
Source:
Talanta 2023 v.252 pp. 123821
ISSN:
0039-9140
Subject:
DNA; Staphylococcus aureus; biosensors; electrodes; gold
Abstract:
... As one of the major foodborne pathogens, Staphylococcus aureus (S. aureus) can cause infectious diseases. In the current study, a novel electrochemical biosensor based on saltatory rolling circle amplification (SRCA) combined with CRISPR/Cas12a system was developed for the accurate detection of S. aureus. The thio-modified reporter probes (SH-ssDNA-MB) was immobilized on the surface of gold nanopa ...
DOI:
10.1016/j.talanta.2022.123821

32. A novel label-free universal biosensing platform based on CRISPR/Cas12a for biomarker detection

Author:
Xiaomei Mu; Xin Wang; Yuxin Qin; Yong Huang; Jianniao Tian; Shulin Zhao
Source:
Talanta 2023 v.251 pp. 123795
ISSN:
0039-9140
Subject:
DNA; biomarkers; biosensors; disease surveillance; enzyme activity; fluorescence; hydrolysis; microRNA; nanosilver
Abstract:
... The development of a biosensing platform with high sensitivity, high specificity, and low cost for the detection of biomarkers, especially one that is programmable and universal, is critical for disease surveillance and diagnosis, yet it remains a difficulty. Herein, we combined the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system with a fluorescent label-free biosenso ...
DOI:
10.1016/j.talanta.2022.123795

33. A novel method for detection of ochratoxin A in foods—Co-MOFs based dual signal ratiometric electrochemical aptamer sensor coupled with DNA walker

Author:
Yan Guan; Peng-Bin Si; Tong Yang; Yuan Wu; Yun-Hui Yang; Rong Hu
Source:
Food chemistry 2023 v.403 pp. 134316
ISSN:
0308-8146
Subject:
DNA; cobalt; detection limit; electrochemistry; electrodes; enzyme-linked immunosorbent assay; food chemistry; human health; kidneys; ochratoxin A; oligonucleotides; red wines; toluidine blue
Abstract:
... Ochratoxin A (OTA) is a naturally occurring mycotoxin that poses serious threats, such as kidney damage, to human health. Therefore, we developed a DNA walker-based dual-signal electrochemical ratiometric platform for OTA detection, which could overlook the variations in environmental and instrumental factors and DNA load densities. Cobalt metal–organic frameworks (Co-MOFs) and toluidine blue were ...
DOI:
10.1016/j.foodchem.2022.134316

34. The regulatory roles of DDIT4 in TDCIPP-induced autophagy and apoptosis in PC12 cells

Author:
Li Li; Lingyi Xi; Jin Wu; Zunquan Zhao; Youliang Chen; Weili Liu; Zhihui Pan; Mingzhu Liu; Danfeng Yang; Zhaoli Chen; Yanjun Fang
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 823-830
ISSN:
1001-0742
Subject:
DNA; Western blotting; apoptosis; autophagy; flame retardants; flow cytometry; humans; neurons; neurotoxicity; phosphates; phosphorylation; protective effect; rats; China
Abstract:
... Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is a commonly used organophosphate-based flame retardant and can bio-accumulate in human tissues and organs. As its structure is similar to that of neurotoxic organophosphate pesticides, the neurotoxicity of TDCIPP has raised widespread concerns. TDCIPP can increase neuronal apoptosis and induce autophagy. However, its regulatory mechanism remains un ...
DOI:
10.1016/j.jes.2022.02.046

35. 100 Years of Chromosome Research in Rye, Secale L.

Author:
Rolf Schlegel
Source:
Plants 2022 v.11 no.13 pp. -
ISSN:
2223-7747
Subject:
DNA; barley; cytogenetic analysis; fish; fluorescence; genes; grasses; haploidy; meiosis; microscopy; mitosis; reciprocal translocation; rice; rye; sequence analysis; tetraploidy; trisomics; wheat
Abstract:
... Although microscopy and genetics were still in their infancy, there are cytological results produced a hundred years ago that are still relevant today. Since the 1920s, rye has been a subject of chromosome research. It started by plotting its mitotic and meiotic chromosomes to determine genome size. After controversial evidence, it became clear that the base number is n = 7. However, structural di ...
DOI:
10.3390/plants11131753

36. 19th century family saga re-told by DNA recovered from postcard stamps

Author:
Cordula Haas; Christian Körner; Andrea Sulzer; Adelgunde Kratzer
Source:
Forensic science international 2022 v.330 pp. 111129
ISSN:
0379-0738
Subject:
DNA; children; forensic sciences; males; saliva
Abstract:
... Old postcards with stamps might help unravelling historical family stories and relationships. By employing ancient DNA recovered from world war I postage stamps, we disprove a family saga of an illegitimate child born in 1887. We developed a protocol to collect DNA from saliva, trapped and protected on the backside of postage stamps glued on postcards. With replicate STR analyses we were able to a ...
DOI:
10.1016/j.forsciint.2021.111129

37. 2,7-Diazapyrenes: a brief review on synthetic strategies and application opportunities

Author:
Anindita Mukherjee; Alexey A. Akulov; Sougata Santra; Mikhail V. Varaksin; Grigory A. Kim; Dmitry S. Kopchuk; Olga S. Taniya; Grigory V. Zyryanov; Oleg. N. Chupakhin
Source:
RSC advances 2022 v.12 no.15 pp. 9323-9341
ISSN:
2046-2069
Subject:
DNA; electronics; geometry; moieties
Abstract:
... 2,7-Diazapyrenes are promising azaaromatic scaffolds with a unique structural geometry and supramolecular properties. This core moiety and its derivatives with some N-methyl cations like N-methyl-2,7,-diazapyrenium, and N,N′-dimethyl-2,7-diazapyrenium attract special attention due to their challenging photophysical properties, especially in the context of interactions with DNA and some of its mono ...
DOI:
10.1039/d2ra00260d

38. 2-Oxopurine Riboside: A Dual Fluorescent Analog and Photosensitizer for RNA/DNA Research

Author:
Naishka E. Caldero-Rodríguez; Enrique M. Arpa; Diego J. Cárdenas; Lara Martínez-Fernández; Steffen Jockusch; Sourav Kanti Seth; Inés Corral; Carlos E. Crespo-Hernández
Source:
Journal of physical chemistry 2022 v.126 no.24 pp. 4483-4490
ISSN:
1520-5207
Subject:
DNA; RNA; absorption; aqueous solutions; fluorescence; nucleosides; photolysis; photosensitizing agents; quantum mechanics; singlet oxygen; solvents
Abstract:
... There is significant interest in developing suitable nucleoside analogs exhibiting high fluorescence and triplet yields to investigate the structure, dynamics, and binding properties of nucleic acids and promote selective photosensitized damage to DNA/RNA, respectively. In this study, steady-state, laser flash photolysis, time-resolved IR luminescence, and femtosecond broad-band transient absorpti ...
DOI:
10.1021/acs.jpcb.2c01113

39. 2D DNA lattices assembled from DX-coupled tiles

Author:
Wei Zhang; Chuan Jiang; Xin Guo; Mirza Muhammad Faran Ashraf Baig; Caihong Ni; Shou-Jun Xiao
Source:
Journal of colloid and interface science 2022 v.616 pp. 499-508
ISSN:
0021-9797
Subject:
DNA; atomic force microscopy; mechanics; mica; oligonucleotides
Abstract:
... Formation of coupled double crossovers (DXs) in a circular 106-mer oligonucleotide (c106nt) could generate stable tiles with the tile core span of 10 half turns. The large-span tiles with complicated curvatures and mechanics could assemble 2D lattices under different environments. Hence, 2D DNA lattice structures based on tile types and sequences, tile packing modes of corrugation and non-corrugat ...
DOI:
10.1016/j.jcis.2022.02.038

40. 2q35-rs13387042 variant and the risk of breast cancer: a case–control study

Author:
Abolfazl Nesaei; Zari Naderi Ghale-noie; Asma Khorshid Shamshiri; Fahimeh Afzaljavan; Mahdi Rivandi; Amir Tajbakhsh; Fatemeh Homaei Shandiz; Alireza Pasdar
Source:
Molecular biology reports 2022 v.49 no.5 pp. 3549-3557
ISSN:
0301-4851
Subject:
DNA; alleles; blood; breast neoplasms; breasts; carcinogenesis; case-control studies; demographic statistics; etiology; genetic background; genotyping; histopathology; lifestyle; molecular biology; risk; risk assessment; statistical analysis
Abstract:
... BACKGROUND: Breast Cancer is the most frequent neoplasm diagnosed among women worldwide. Genetic background and lifestyle/environment play a significant role in the disease etiology. According to Genome-wide association studies, some single-nucleotide polymorphisms such as 2q35-rs13387042–(G/A) have been introduced to be associated with breast cancer risk and features. In this study, we aimed to e ...
DOI:
10.1007/s11033-022-07195-6

41. A 5-color flow cytometry panel to assess plasma membrane integrity, acrosomal status, membrane lipid organization and mitochondrial activity of boar and stallion spermatozoa following liquid semen storage

Author:
Monike Quirino; Ulrike Jakop; Ana Paula Gonçalves Mellagi; Fernando Pandolfo Bortolozzo; Markus Jung; Martin Schulze
Source:
Animal reproduction science 2022 v.247 pp. 107076
ISSN:
0378-4320
Subject:
DNA; animal reproduction; boars; flow cytometry; fluorescent dyes; lipids; liquids; mitochondria; plasma membrane; propidium; rhodamines; semen; sperm quality; stallions
Abstract:
... For a more practically applicable analysis of different sperm characteristics, this study aimed to develop a 5-color flow cytometry (FC) panel to concurrently analyze four sperm parameters in liquid boar and stallion semen, using also a DNA-marker for selecting sperm cell events. From each of thirty extended boar semen doses and twelve stallion semen doses, six aliquots were taken. For evaluating ...
DOI:
10.1016/j.anireprosci.2022.107076

42. A 638‐gene phylogeny supports the recognition of twice as many species in the Malagasy endemic genus Capurodendron (Sapotaceae)

Author:
Carlos G. Boluda; Camille Christe; Yamama Naciri; Laurent Gautier
Source:
Taxon 2022 v.71 no.2 pp. 360-395
ISSN:
0040-0262
Subject:
DNA; Sapotaceae; conservation status; extinction; hardwood; new species; phylogeny; risk; statistical analysis; Madagascar
Abstract:
... The Malagasy genus Capurodendron currently accommodates 26 described species and is the largest genus of the family Sapotaceae in Madagascar. These species are frequently logged because of their valued hardwood, which potentially puts them at risk of extinction. Species‐level identifications are often problematic, and this hinders both an accurate assessment of their conservation status and the de ...
DOI:
10.1002/tax.12676

43. An 82 bp tandem repeat family typical of 3′ non-coding end of Gypsy/TAT LTR retrotransposons is conserved in Coffea spp. pericentromeres

Author:
Leonardo Adabo Cintra; Thaíssa Boldieri de Souza; Letícia Maria Parteka; Lucas Mesquita Barreto; Luiz Filipe Protasio Pereira; Marcos Letaif Gaeta; Romain Guyot; André Luís Laforga Vanzela
Source:
Genome 2022 v.65 no.3 pp. 137-151
ISSN:
1480-3321
Subject:
DNA; allotetraploidy; centromeres; chromatin; genome; microsatellite repeats; non-coding RNA; retrotransposons
Abstract:
... Coffea spp. chromosomes are very small and accumulate a variety of repetitive DNA families around the centromeres. However, the proximal regions of Coffea chromosomes remain poorly understood, especially regarding the nature and organisation of the sequences. Taking advantage of the genome sequences of C. arabica (2n = 44), C. canephora, and C. eugenioides (C. arabica progenitors with 2n = 22) and ...
DOI:
10.1139/gen-2021-0045

44. 90-day nose-only inhalation toxicity study of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in Sprague-Dawley rats

Author:
Shu-Chieh Hu; Seonggi Min; Hyun-Ki Kang; Dong-Jin Yang; Mallikarjuna Basavarajappa; Sherry M. Lewis; Kelly J. Davis; Ralph E. Patton; Matthew S. Bryant; Estatira Sepehr; Raul Trbojevich; Mason G. Pearce; Michelle E. Bishop; Wei Ding; Robert H. Heflich; MacKean P. Maisha; Robert Felton; Susan Chemerynski; Steven B. Yee; Melis Coraggio; Hans Rosenfeldt; R. Philip Yeager; Paul C. Howard; Yunan Tang
Source:
Food and chemical toxicology 2022 v.160 pp. 112780
ISSN:
0278-6915
Subject:
DNA; aerosols; air; biomarkers; blood; body weight; bone marrow; breathing; carcinogenesis; carcinogenicity; cigarettes; comet assay; food consumption; genotoxicity; histopathology; humans; inhalation toxicity; lowest observed effect level; lungs; metabolites; mutagens; nitrosamines; no observed adverse effect level; nose; propylene glycol; urine
Abstract:
... 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is one of the key tobacco-specific nitrosamines that plays an important role in human lung carcinogenesis. Repeated dose inhalation toxicity data on NNK, particularly relevant to cigarette smoking, however, is surprisingly limited. Hence, there is a lack of direct information available on the carcinogenic and potential non-carcinogenic effects o ...
DOI:
10.1016/j.fct.2021.112780

45. AAQSP increases mapping resolution of stable QTLs through applying NGS-BSA in multiple genetic backgrounds

Author:
Xiaoyu Wang; Xiaowei Zhang; Daoran Fan; Juwu Gong; Shaoqi Li; Yujie Gao; Aiying Liu; Linjie Liu; Xiaoying Deng; Yuzhen Shi; Haihong Shang; Yuanming Zhang; Youlu Yuan
Source:
Theoretical and applied genetics 2022 v.135 no.9 pp. 3223-3235
ISSN:
0040-5752
Subject:
DNA; Gossypium hirsutum; chromosome mapping; chromosomes; gene expression; genetic background; genetic improvement; heterosis; heterozygosity; lint cotton; nucleotide sequences; phenotypic variation
Abstract:
... KEY MESSAGE : In this study, we present AAQSP as an extension of existing NGS-BSA applications for identifying stable QTLs at high resolution. GhPAP16 and GhIQD14 fine mapped on chromosome D09 of upland cotton are identified as important candidate genes for lint percentage (LP). Bulked segregant analysis combined with next generation sequencing (NGS-BSA) allows rapid identification of genome seque ...
DOI:
10.1007/s00122-022-04181-1

46. AAV infection of bovine embryos: Novel, simple and effective tool for genome editing

Author:
Anna S. Krivonogova; Alexandra V. Bruter; Valeria A. Makutina; Yuliya D. Okulova; Leonid A. Ilchuk; Marina V. Kubekina; Alexandra Yu Khamatova; Tatiana V. Egorova; Vladimir S. Mymrin; Yuliya Yu Silaeva; Alexey V. Deykin; Maxim A. Filatov; Albina G. Isaeva
Source:
Theriogenology 2022 v.193 pp. 77-86
ISSN:
0093-691X
Subject:
DNA; animal reproduction; blastocyst; cattle; genetically modified organisms; genome; lectins; macrophages; serotypes
Abstract:
... Adeno-associated viruses (AAV) are widely used in the field of genetically modified organism production. In this work, transduction of bovine embryos by AAV was selected as a potential approach to perform genetic modifications: we have used recombinant AAV to produce GFP-positive bovine embryos. Five different AAV serotypes were used to evaluate their ability to deliver genetic material into the b ...
DOI:
10.1016/j.theriogenology.2022.09.007

47. AKT1 mediates multiple phosphorylation events that functionally promote HSF1 activation

Author:
Wen‐Cheng Lu; Ramsey Omari; Haimanti Ray; John Wang; Imade Williams; Curteisha Jacobs; Natasha Hockaden; Matthew L. Bochman; Richard L. Carpenter
Source:
FEBS journal 2022 v.289 no.13 pp. 3876-3893
ISSN:
1742-464X
Subject:
DNA; genes; heat shock proteins; heat shock response; heat stress; mass spectrometry; phosphorylation; phosphotransferases (kinases); proteome; transcription (genetics); transcription factors; transcriptional activation
Abstract:
... The heat stress response activates the transcription factor heat shock factor 1 (HSF1), which subsequently upregulates heat shock proteins to maintain the integrity of the proteome. HSF1 activation requires nuclear localization, trimerization, DNA binding, phosphorylation and gene transactivation. Phosphorylation at S326 is an important regulator of HSF1 transcriptional activity. Phosphorylation a ...
DOI:
10.1111/febs.16375

48. ATP Purinergic Receptor P2X1-Dependent Suicidal NETosis Induced by Cryptosporidium parvum under Physioxia Conditions

Author:
Seyed Sajjad Hasheminasab; Iván Conejeros; Zahady D. Velásquez; Tilman Borggrefe; Ulrich Gärtner; Faustin Kamena; Anja Taubert; Carlos Hermosilla
Source:
Biology 2022 v.11 no.3 pp. -
ISSN:
2079-7737
Subject:
Cryptosporidium parvum; DNA; Protozoa; acidification; cattle; cryptosporidiosis; elastase; extrusion; fluorescence microscopy; glucose; glycolysis; helminths; hyperoxia; intestines; lactic acid; neutrophils; oocysts; oxygen; oxygen consumption; plasma membrane; purinergic receptors; sporozoites; wildlife
Abstract:
... Cryptosporidiosis is a zoonotic intestinal disease that affects humans, wildlife, and neonatal cattle, caused by Cryptosporidium parvum. Neutrophil extracellular traps (NETs), also known as suicidal NETosis, are a powerful and ancient innate effector mechanism by which polymorphonuclear neutrophils (PMN) battle parasitic organisms like protozoa and helminths. Here, C. parvum oocysts and live sporo ...
DOI:
10.3390/biology11030442

49. ATP-Responsive Strand Displacement Coupling with DNA Origami/AuNPs Strategy for the Determination of Microcystin-LR Using Surface-Enhanced Raman Spectroscopy

Author:
Bingyang Huo; Ling Xia; Zhixian Gao; Gongke Li; Yuling Hu
Source:
Analytical chemistry 2022 v.94 no.34 pp. 11889-11897
ISSN:
1520-6882
Subject:
DNA; Raman spectroscopy; adenosine triphosphate; analytical chemistry; detection limit; liquid chromatography; microcystin-LR; nanomaterials; oligonucleotides; solid phase extraction; tandem mass spectrometry
Abstract:
... The DNA origami-mediated self-assembly strategy has emerged as a powerful tool in surface-enhanced Raman spectroscopy (SERS). However, these self-assembly approaches typically do not possess high detection specificity. Herein, a novel strategy based on adenosine triphosphate (ATP)-responsive strand displacement (ARSD) coupling with DNA origami/AuNPs for SERS analysis of microcystin-LR (MC-LR) is p ...
DOI:
10.1021/acs.analchem.2c02440

50. ATR-FTIR spectroscopy as a pre-screening technique for the PMI assessment and DNA preservation in human skeletal remains – A review

Author:
Leskovar Tamara; Zupanič Pajnič Irena; Jerman Ivan; Črešnar Matija
Source:
Quaternary international 2022 pp. -
ISSN:
1040-6182
Subject:
DNA; archaeology; chemometrics; collagen; forensic sciences; humans; postmortem changes; prediction; spectroscopy
Abstract:
... Skeletal remains from archaeological and forensic contexts are commonly used for various analyses, including DNA. Our research investigates relatively fast and inexpensive ATR-FTIR spectroscopy with further data manipulation as a potential pre-screening technique for assessing the preservation state of skeletal remains. We seek to correlate spectra and chemometric indices with the biological, chem ...
DOI:
10.1016/j.quaint.2022.09.004