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- Reis, Levi Eduardo Soares, et al. Show all 9 Authors
- Parasitology research 2018 v.117 no.10 pp. 3341-3346
- DNA; Leishmania infantum; blood; blood sampling; diagnostic techniques; dogs; epidemiological studies; hosts; parasites; quantitative polymerase chain reaction; spleen; tissues; veterinary clinics; visceral leishmaniasis
- ... Dogs are important hosts and reservoirs of leishmaniasis, a disease caused by protozoan parasites from the genus Leishmania, affecting ~12 million people worldwide. The detection of visceral leishmaniasis (VL) in dogs by real-time PCR (qPCR) may improve on diagnosis, but the different qPCR methods available for Leishmania DNA detection have not been established as routine in diagnostic tools and/o ...
- Reis, Levi Eduardo Soares, et al. Show all 12 Authors
- Veterinary parasitology 2013 v.197 no.3-4 pp. 498-503
- DNA; DNA-directed DNA polymerase; Leishmania infantum; bone marrow; dogs; ears; enzyme-linked immunosorbent assay; fluorescent antibody technique; genes; parasites; quantitative polymerase chain reaction; spleen; visceral leishmaniasis
- ... Polymerase chain reaction (PCR) and its variations represent highly sensitive and specific methods for Leishmania DNA detection and subsequent canine visceral leishmaniasis (CVL) diagnosis. The aim of this work was to compare three different molecular diagnosis techniques (conventional PCR [cPCR], seminested PCR [snPCR], and quantitative PCR [qPCR]) in samples of skin and spleen from 60 seropositi ...