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1. Occurrence and multilocus genotyping of Giardia duodenalis in black-boned sheep and goats in southwestern China

Author:
Zou, Feng-Cai,   et al.   ; Chen, Dan; Zou, Yang; Li, Zhao; Wang, Sha-Sha; Xie, Shi-Chen; Shi, Lian-Qin; Yang, Jian-Fa; Zhao, Guang-Hui; Zhu, Xing-Quan;     Show all 10 Authors
Source:
Parasites & vectors 2019 v.12 no.1 pp. 102
ISSN:
1756-3305
Subject:
DNA; Giardia lamblia; breeds; feces; genes; genetic variation; genotype; genotyping; giardin protein; glutamate dehydrogenase; goats; host range; humans; intestines; loci; polymerase chain reaction; sheep; sheep diseases; triose-phosphate isomerase; China
Abstract:
... BACKGROUND: Giardia duodenalis is an important intestinal protozoan infecting both humans and animals, causing significant public health concern and immeasurable economic losses to animal husbandry. Sheep and goats have been reported as common reservoirs of G. duodenalis, but only a limited amount of information is available for native breeds of these small ruminants in China. The present study in ...
DOI:
10.1186/s13071-019-3367-1

2. Molecular detection and genetic characterization of Toxoplasma gondii infection in sika deer (Cervus nippon) in China

Author:
Zou, Feng-Cai,   et al.   ; Cong, Wei; Qin, Si-Yuan; Meng, Qing-Feng; Qian, Ai-Dong; Zhu, Xing-Quan;     Show all 6 Authors
Source:
Infection, genetics, and evolution 2016 v.39 pp. 9-11
ISSN:
1567-1348
Subject:
Cervus nippon; DNA; Toxoplasma gondii; genes; genetic markers; genetic variation; genotype; genotyping; hosts; human health; lungs; polymerase chain reaction; restriction fragment length polymorphism; tissues; toxoplasmosis; China
Abstract:
... The objective of the present study was to investigate the prevalence and genetic characterization of Toxoplasma gondii infection in sika deer in China. During August 2014 to November 2014, a total of 450 tissue samples coming from 150 sika deer were collected to detect the T. gondii B1 gene using a nested PCR, and the positive samples were genotyped at 11 genetic markers (SAG1, 5′- and 3′-SAG2, al ...
DOI:
10.1016/j.meegid.2016.01.005

3. Genetic characterization of Toxoplasma gondii in Yunnan black goats (Capra hircus) in southwest China by PCR-RFLP

Author:
Zou, Feng-Cai,   et al.   ; Miao, Qiang; Huang, Si-Yang; Qin, Si-Yuan; Yu, Xin; Yang, Yan; Yang, Jian-Fa; Zhu, Xing-Quan;     Show all 8 Authors
Source:
Parasites & vectors 2015 v.8 no.1 pp. 57
ISSN:
1756-3305
Subject:
DNA; Toxoplasma gondii; genes; genetic markers; genetic variation; genotype; goats; humans; liver; loci; lymph nodes; parasites; polymerase chain reaction; public health; restriction fragment length polymorphism; China
Abstract:
... BACKGROUND: Toxoplasma gondii is a protozoan parasite that infects almost all warm-blooded animals and human beings. Goats are one of the susceptible animals to T. gondii. However, little is known of genetic diversity of T. gondii in Yunnan black goats in China. The objective of this present study was to determine the genotypes of T. gondii isolates from black goats in Yunnan province, southwest C ...
DOI:
10.1186/s13071-015-0673-0
PubMed:
25622613
PubMed Central:
PMC4316756

4. Sequence and comparative analysis of the MIP gene in Chinese straw mushroom, Volvariella volvacea

Author:
Zou, Feng,   et al.   ; Chen, Bing-Zhi; Gui, Fu; Xie, Bao-Gui; Jiang, Yu-Ji; Deng, You-Jin;     Show all 6 Authors
Source:
Genome 2012 v.55 no.9 pp. 667-672
ISSN:
1480-3321
Subject:
DNA; Volvariella volvacea; computer software; fungi; genes; nucleotide sequences; phylogeny
Abstract:
... The mitochondrial intermediate peptidase (MIP) gene is conserved in fungi. It is linked closely with the mating-type A (mtA) gene. In this study, a fragment of the MIP gene in Volvariella volvacea (Bull. ex Fr.) Singer was first cloned by homologue-based cloning technology. Subsequently, the entire MIP DNA sequence (PYd21-MIP) was obtained after the fragment was compared with the genomic data thro ...
DOI:
10.1139/g2012-043

5. Genetic characterization of Toxoplasma gondii from cats in Yunnan Province, Southwestern China

Author:
Zou, Feng-Cai,   et al.   ; Tian, Yi-Ming; Huang, Si-Yang; Miao, Qiang; Jiang, Hai-Hai; Yang, Jian-Fa; Su, Chunlei; Zhu, Xing-Quan;     Show all 8 Authors
Source:
Parasites & vectors 2014 v.7 no.1 pp. 178
ISSN:
1756-3305
Subject:
DNA; Toxoplasma gondii; brain; cats; genes; genetic markers; genetic variation; genotype; heart; hosts; liver; loci; parasites; polymerase chain reaction; public health; restriction fragment length polymorphism; tongue; China
Abstract:
... BACKGROUND: Cats are the definitive hosts of Toxoplasma gondii. The distribution of genetic diversity of T. gondii in cats is of importance to understand the transmission of this parasite. The objective of this study was to genetically characterize T. gondii isolates from cats in Yunnan province, southwestern China. METHODS: Genomic DNA was extracted from 5–10 g cat tissue samples (brain, tongue, ...
DOI:
10.1186/1756-3305-7-178
PubMed:
24725959
PubMed Central:
PMC3990027

6. Identification and characterization of new major sperm protein genes from Oesophagostomum dentatum and Oesophagostomum quadrispinulatum from pigs in China

Author:
Zou, Feng-Cai,   et al.   ; Lin, Rui-Qing; Zhou, Dong-Hui; Huang, Si-Yang; Zhang, Yuan; Song, Hui-Qun; Weng, Ya-Biao; Zhu, Xing-Quan;     Show all 8 Authors
Source:
Experimental parasitology 2013 v.133 no.2 pp. 187-192
ISSN:
0014-4894
Subject:
Ascaris suum; DNA; Oesophagostomum dentatum; Oesophagostomum quadrispinulatum; amino acid sequences; amino acids; introns; molecular biology; multigene family; parasitology; polymerase chain reaction; reproduction; sequence analysis; spermatozoa; swine; China
Abstract:
... The present study identified and characterized new major sperm protein (MSP) genes from the two nodule worms Oesophagostomum dentatum and Oesophagostomum quadrispinulatum collected from pigs in China. Total genomic DNA was extracted individually from 10 male nematode samples representing O. dentatum, and 4 male nematode samples representing O. quadrispinulatum. A pair of primers (OMSP1F/MSP1R) was ...
DOI:
10.1016/j.exppara.2012.11.021

7. Identification and functional analysis of the cathepsin D gene promoter of Bombyx mori

Author:
Zou, Feng-Ming,   et al.   ; Yu, Jie; Wu, Feng-Yao; Cai, Xiang-Yun; Yu, Hai-Yan; Liu, Yan-Wei; Fang, Yin; Ren, Zi-Xu; Jia, Jun-Qiang; Zhang, Guo-Zheng; Guo, Xi-Jie; Jin, Byung-Rae; Gui, Zhong-Zheng;     Show all 13 Authors
Source:
Molecular biology reports 2014 v.41 no.3 pp. 1623-1630
ISSN:
0301-4851
Subject:
Autographa californica multiple nucleopolyhedrovirus; Bombyx mori; DNA; apoptosis; cathepsin D; digestive system; fat body; genes; larvae; metamorphosis; promoter regions; silkworms; transcription (genetics)
Abstract:
... The gene encoding cathepsin D of silkworm, Bombyx mori (BmCatD) is specifically expressed in the larval fat body and pupal gut, and plays an important role in the programmed cell death during metamorphosis. To identify element involved in this transcription-dependent spatial restriction, truncation and deletion of the 5′ terminal from the BmCatD promoter were conducted in vivo. The recombinant AcM ...
DOI:
10.1007/s11033-013-3009-1

8. First Report of Genotyping of Toxoplasma gondii Isolates From Wild Birds in China

Author:
Zou, Feng-Cai,   et al.   ; Huang, Si-Yang; Cong, Wei; Zhou, Peng; Zhou, Dong-Hui; Wu, Song-Ming; Xu, Min-Jun; Song, Hui-Qun; Zhu, Xing-Quan;     Show all 9 Authors
Source:
The Journal of parasitology 2012 v.98 no.3 pp. 681-682
ISSN:
0022-3395
Subject:
DNA; Passer domesticus; Passer montanus; Phasianus colchicus; Toxoplasma gondii; breast muscle; genes; genetic markers; genetic variation; genotype; genotyping; human health; loci; parasites; pheasants; polymerase chain reaction; surveys; wild birds; China; Europe; North America; South America
Abstract:
... Toxoplasma gondii is an important cosmopolitan opportunistic protozoan parasite, which threatens the health of human beings and animals. Genetic characterization of isolates from South America has revealed high genetic diversity. In contrast, isolates from North America and Europe were highly clonal, with 3 major lineages known as the Types I, II, and III. However, limited information on T. gondii ...
DOI:
10.1645/GE-3038.1

9. Electrophoretic detection of genetic variability among Schistosoma japonicum isolates by sequence-related amplified polymorphism

Author:
Zou, Feng-Cai,   et al.   ; Song, Hui-Qun; Mo, Xi-Hao; Zhao, Guang-Hui; Li, Juan; Liu, Wei; Wu, Xiang-Yun; Lin, Rui-Qing; Weng, Ya-Biao; Zhu, Xing-Quan;     Show all 10 Authors
Source:
Electrophoresis 2011 v.32 no.11 pp. 1364-1370
ISSN:
0173-0835
Subject:
DNA; Schistosoma japonicum; Schistosoma mansoni; algorithms; electrophoresis; gels; genetic variation; lakes; polymerase chain reaction; China; Puerto Rico
Abstract:
... In the present study, sequence-related amplification polymorphism (SRAP) was utilized to study the genetic variability among Schistosoma japonicum isolates from different provinces in China, using Schistosoma mansoni from Puerto Rico for comparison. Five out of ten tested SRAP primer combinations displayed significant polymorphisms among S. japonicum isolates from China, namely ME2/EM1, ME4/EM1, M ...
DOI:
10.1002/elps.201000600

10. IRAP: An efficient retrotransposon-based electrophoretic technique for studying genetic variability among geographical isolates of Schistosoma japonicum

Author:
Zou, Feng-Cai,   et al.   ; Li, Juan; Zhao, Guang-Hui; Li, Xiao-Yan; Chen, Fen; Chen, Jing-Bin; Yang, Jian-Fa; Lin, Rui-Qing; Weng, Ya-Biao; Zhu, Xing-Quan;     Show all 10 Authors
Source:
Electrophoresis 2011 v.32 no.12 pp. 1473-1479
ISSN:
0173-0835
Subject:
DNA; Schistosoma japonicum; electrophoresis; females; genetic variation; lakes; males; parasites; provenance; retrotransposons; sexual development; China
Abstract:
... In the present study, a inter-retrotransposon-amplified polymorphism (IRAP) technique, based on retrotransposons, was used to examine genetic variability among Schistosoma japonicum isolates from different provinces in mainland China. Of the 15 primers screened, 5 produced highly reproducible IRAP patterns. Using these primers, 54 discernible DNA fragments were generated with 40 (74.07%) being pol ...
DOI:
10.1002/elps.201100044

11. A specific PCR assay for the identification and differentiation of Schistosoma japonicum geographical isolates in mainland China based on analysis of mitochondrial genome sequences

Author:
Zou, Feng-Cai,   et al.   ; Zhao, Guang-Hui; Li, Juan; Song, Hui-Qun; Li, Xiao-Yan; Chen, Fen; Lin, Rui-Qing; Yuan, Zi-Guo; Weng, Ya-Biao; Hu, Min; Zhu, Xing-Quan;     Show all 11 Authors
Source:
Infection, genetics, and evolution 2012 v.12 no.5 pp. 1027-1036
ISSN:
1567-1348
Subject:
DNA; Schistosoma japonicum; amino acids; gels; genes; humans; microsatellite repeats; monitoring; parasites; phylogeny; polyacrylamide; polymerase chain reaction; ribosomal RNA; sequence analysis; China; Yangtze River
Abstract:
... In the present study, near-complete mt genome sequences for eight representative Schistosoma japonicum samples from seven endemic provinces in mainland China were analyzed. Sequence differences among the eight mt genomes of S. japonicum samples were 0.20–2.51%. Variation in protein-coding genes was greater than that in rRNA genes. The mt DNA sequences of S. japonicum samples from south-western (SW ...
DOI:
10.1016/j.meegid.2012.02.020
PubMed:
22446475

12. An effective sequence characterized amplified region-PCR method derived from restriction site-amplified polymorphism for the identification of female Schistosoma japonicum of zoonotic significance

Author:
Zou, Feng-Cai,   et al.   ; Zhao, Guang-Hui; Li, Juan; Lin, Rui-Qing; Liu, Wei; Yuan, Zi-Guo; Mo, Xi-Hao; Song, Hui-Qun; Weng, Ya-Biao; Zhu, Xing-Quan;     Show all 10 Authors
Source:
Electrophoresis 2010 v.31 no.4 pp. 641-647
ISSN:
0173-0835
Subject:
Clonorchis sinensis; DNA; Fasciola hepatica; Schistosoma japonicum; electrophoresis; epidemiological studies; females; genetic variation; humans; males; parasites; provenance; sequence analysis; sexual development; China
Abstract:
... In the present study, restriction site-amplified polymorphism (RSAP) markers were used to examine the genetic variability of Schistosoma japonicum isolates from different endemic provinces in mainland China. Of the 45 pairs of primers screened, 10 RSAP markers showed a clear banding pattern with good resolution; however, only six exhibited a polymorphism among different isolates. Among six RSAP ma ...
DOI:
10.1002/elps.200900615