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1. Enhancement of autophagy flux by isopsoralen ameliorates interleukin-1β-stimulated apoptosis in rat chondrocytes

Author:
Chen, Zhi; Li, Chen; Qian, Yi-Hong; Fu, Yang; Feng, Zi-Ming
Source:
Journal of Asian natural products research 2020 v.22 no.2 pp. 179-192
ISSN:
1477-2213
Subject:
DNA fragmentation; apoptosis; autophagy; cartilage; caspase-3; chondrocytes; enzyme activity; interleukin-1beta; osteoarthritis; pathogenesis; protective effect; proteins; rats
Abstract:
... Chondrocyte apoptosis contributes to the pathogenesis of cartilage degeneration in osteoarthritis (OA). We found that isopsoralen pretreatment significantly reversed the increase in DNA fragmentation and apoptosis rate, and significantly decreased the caspase-3 activity and PARP cleavage in IL-1β-treated chondrocytes. Isopsoralen pretreatment markedly inhibited disruption of matrix proteins. Moreo ...
DOI:
10.1080/10286020.2018.1537265

2. Effect of euphorbia factor L1 on oxidative stress, apoptosis, and autophagy in human gastric epithelial cells

Author:
Zhu, An; Sun, Yuqing; Zhong, Qianwen; Yang, Jinlan; Zhang, Tao; Zhao, Jingwei; Wang, Qi
Source:
Phytomedicine 2019 v.64 pp. 152929
ISSN:
0944-7113
Subject:
4',6-diamidino-2-phenylindole; DNA; DNA fragmentation; Euphorbia lathyris; apoptosis; autophagy; caspase-3; caspase-9; cell viability; computer simulation; cytochrome c; cytotoxicity; diterpenoids; epithelial cells; gastric mucosa; gene expression regulation; humans; hydrogen bonding; malondialdehyde; mechanism of action; medicinal plants; membrane potential; messenger RNA; mitochondria; mitochondrial membrane; oxidative stress; phosphatidylinositol 3-kinase; protein synthesis; quantitative polymerase chain reaction; reactive oxygen species; reverse transcriptase polymerase chain reaction; staining; superoxide dismutase
Abstract:
... Euphorbia factor L1 (EFL1), a lathyrane-type diterpenoid from the medicinal herb Euphorbia lathyris L. (Euphorbiaceae), has been reported for many decades to induce gastric irritation, but the underlying mechanisms remain unclear.The objective of this study was to investigate EFL1-induced cytotoxicity and the potential mechanisms of action on the human normal gastric epithelial cell GES-1.GES-1 ce ...
DOI:
10.1016/j.phymed.2019.152929

3. RAD51AP1 Is an Essential Mediator of Alternative Lengthening of Telomeres

Author:
Barroso-González, Jonathan; García-Expósito, Laura; Hoang, Song My; Lynskey, Michelle L.; Roncaioli, Justin L.; Ghosh, Arundhati; Wallace, Callen T.; de Vitis, Marco; Modesti, Mauro; Bernstein, Kara A.; Sarkar, Saumendra N.; Watkins, Simon C.; O’Sullivan, Roderick J.
Source:
Molecular cell 2019 v.76 no.1 pp. 11-26.e7
ISSN:
1097-2765
Subject:
DNA damage; DNA fragmentation; DNA replication; apoptosis; autophagy; homologous recombination; lysine; mutation; neoplasm cells; neoplasms; protein content; sumoylation; telomeres
Abstract:
... Alternative lengthening of telomeres (ALT) is a homology-directed repair (HDR) mechanism of telomere elongation that controls proliferation in aggressive cancers. We show that the disruption of RAD51-associated protein 1 (RAD51AP1) in ALT+ cancer cells leads to generational telomere shortening. This is due to RAD51AP1’s involvement in RAD51-dependent homologous recombination (HR) and RAD52-POLD3-d ...
DOI:
10.1016/j.molcel.2019.06.043
PubMed:
31400850
PubMed Central:
PMC6778027

4. The antidepressant clomipramine induces programmed cell death in Leishmania amazonensis through a mitochondrial pathway

Author:
da Silva Rodrigues, Jean Henrique; Miranda, Nathielle; Volpato, Hélito; Ueda-Nakamura, Tânia; Nakamura, Celso Vataru
Source:
Parasitology research 2019 v.118 no.3 pp. 977-989
ISSN:
0932-0113
Subject:
DNA fragmentation; Leishmania amazonensis; adenosine triphosphate; amastigotes; antidepressants; apoptosis; autophagy; endomembrane system; enzymes; homeostasis; leishmaniasis; macrophages; mechanism of action; membrane permeability; metabolism; mitochondria; nitric oxide; oxidative stress; parasites; phosphatidylserines; plasma membrane; promastigotes; shrinkage
Abstract:
... Despite many efforts, the currently available treatments for leishmaniasis are not fully effective. To discover new medications, drug repurposing arises as a promising strategy. We present data that supports the use of the antidepressant clomipramine against Leishmania amazonensis. The drug presented selective activity at micromolar range against both the parasite forms and stimulated nitric oxide ...
DOI:
10.1007/s00436-018-06200-x

5. Ganoderma tsugae induced ROS-independent apoptosis and cytoprotective autophagy in human chronic myeloid leukemia cells

Author:
Hseu, You-Cheng; Shen, Yi-Chun; Kao, Ming-Ching; Mathew, Dony Chacko; Karuppaiya, Palaniyandi; Li, Mei-Ling; Yang, Hsin-Ling
Source:
Food and chemical toxicology 2019 v.124 pp. 30-44
ISSN:
0278-6915
Subject:
DNA fragmentation; Ganoderma tsugae; acetylcysteine; antineoplastic activity; apoptosis; autophagy; cell viability; cytochrome c; death domain receptors; humans; mechanism of action; medicinal fungi; mitochondria; myeloid leukemia; neoplasm cells; reactive oxygen species; Asia
Abstract:
... The medicinal fungus Ganoderma, known in Chinese as Lingzhi or Reishi, traditionally has various medicinal uses and has been employed in cancer treatment in Asia for centuries. This study used ethanol-extracted Ganoderma tsugae (GT) and examined its antitumor activities on human chronic myeloid leukemia cells as well as its molecular mechanism of action. Treatment with GT (200–400 μg/mL) significa ...
DOI:
10.1016/j.fct.2018.11.043

6. Perigestational exposure to low doses of PBDE-47 induces excessive ER stress, defective autophagy and the resultant apoptosis contributing to maternal thyroid toxicity

Author:
Li, Pei; Liu, Luming; Zhou, Guoyu; Tian, Zhiyuan; Luo, Chen; Xia, Tao; Chen, Jingwen; Niu, Qiang; Dong, Lixin; Zhao, Qian; Wang, Aiguo; Zhang, Shun
Source:
The Science of the total environment 2018 v.645 pp. 363-371
ISSN:
0048-9697
Subject:
DNA fragmentation; apoptosis; autophagy; blood serum; brain; caspase-12; caspase-3; endoplasmic reticulum; epithelial cells; females; fetus; flame retardants; gene expression regulation; infants; laboratory animals; mechanism of action; neurotoxicity; progeny; rats; triiodothyronine; unfolded protein response; weaning
Abstract:
... Brominated flame retardant 2,2′,4,4′‑tetrabromodiphenyl ether (PBDE-47) is known to induce developmental neurotoxicity by disturbing thyroid hormones (THs). Evidence shows that maternal THs are crucial for brain development and growth of fetuses and infants. However, little is known about the effects of PBDE-47 on maternal thyroid status and its mode of action. Here, using female Sprague-Dawley ra ...
DOI:
10.1016/j.scitotenv.2018.07.138

7. Phytol isolated from watermelon (Citrullus lanatus) sprouts induces cell death in human T-lymphoid cell line Jurkat cells via S-phase cell cycle arrest

Author:
Itoh, Tomohiro; Ono, Akito; Kawaguchi, Kaori; Teraoka, Sayaka; Harada, Mayo; Sumi, Keitaro; Ando, Masashi; Tsukamasa, Yasuyuki; Ninomiya, Masayuki; Koketsu, Mamoru; Hashizume, Toshiharu
Source:
Food and chemical toxicology 2018 v.115 pp. 425-435
ISSN:
0278-6915
Subject:
Citrullus lanatus; DNA fragmentation; T-lymphocytes; Western blotting; adenocarcinoma; apoptosis; autophagy; cell cycle checkpoints; cyclins; cyclophosphamide; epithelial cells; flow cytometry; human cell lines; humans; in vivo studies; leukemia; lungs; mice; mitogen-activated protein kinase; models; necrosis; reactive oxygen species; therapeutics; toxicology; watermelons
Abstract:
... The phytol isolated from watermelon (Citrullus lanatus) sprouts inhibited the growth of a human T-cell leukemia line Jurkat cell and suppressed tumor progression in a xenograft model of human lung adenocarcinoma epithelial cell line A549 in nude mice. To elucidate the mechanisms underlying the phytol-induced cell death in the present study, we examined the changes in cell morphology, DNA fragmenta ...
DOI:
10.1016/j.fct.2018.03.033

8. Ameliorative effects of selenium on arsenic-induced cytotoxicity in PC12 cells via modulating autophagy/apoptosis

Author:
Rahman, Md. Mostafizur; Uson-Lopez, Rachael A.; Sikder, Md. Tajuddin; Tan, Gongxun; Hosokawa, Toshiyuki; Saito, Takeshi; Kurasaki, Masaaki
Source:
Chemosphere 2018 v.196 pp. 453-466
ISSN:
0045-6535
Subject:
DNA fragmentation; Western blotting; antagonism; antioxidant activity; apoptosis; arsenic; atomic absorption spectrometry; autophagy; caspases; cell viability; chemoprevention; cytotoxicity; human diseases; metals; neoplasms; oxidative stress; reverse transcriptase polymerase chain reaction; selenium; signal transduction; synergism; ubiquitin
Abstract:
... Arsenic is well known toxicant responsible for human diseases including cancers. On the other hand, selenium is an essential trace element with significant chemopreventive effects, anticancer potentials and antioxidant properties. Although previous studies have reported antagonism/synergism between arsenic and selenium in biological systems, the biomolecular mechanism/s is still inconclusive. Ther ...
DOI:
10.1016/j.chemosphere.2017.12.149

9. Inhibitory effects of selenium on cadmium-induced cytotoxicity in PC12 cells via regulating oxidative stress and apoptosis

Author:
Binte Hossain, Kaniz Fatima; Rahman, Md. Mostafizur; Sikder, Md. Tajuddin; Saito, Takeshi; Hosokawa, Toshiyuki; Kurasaki, Masaaki
Source:
Food and chemical toxicology 2018 v.114 pp. 180-189
ISSN:
0278-6915
Subject:
DNA fragmentation; Western blotting; apoptosis; autophagy; cadmium; cadmium chloride; cell viability; cytochrome c; cytosol; cytotoxicity; glutathione; glutathione peroxidase; lactate dehydrogenase; mitochondria; mitogen-activated protein kinase; oxidative stress; selenium; sodium selenite; toxicity testing
Abstract:
... Purpose of this study is to investigate mechanism/s of cyto-protection by selenium (Na2SeO3; Se4+) against cadmium (CdCl2; Cd2+)-induced cytotoxicity using PC12 cells. In addition, Se (5, 10, 20 and 40 μM) and Cd (2.5, 5 and 10 μM)-induced cytotoxicity is determined. Cytotoxicity assays and western blot analyses confirmed that Se (≥10 μM) promotes autophagic cell death via inhibition of mTOR activ ...
DOI:
10.1016/j.fct.2018.02.034

10. Autophagy inhibition enhances anticancer efficacy of artepillin C, a cinnamic acid derivative in Brazilian green propolis

Author:
Endo, Satoshi; Hoshi, Manami; Matsunaga, Toshiyuki; Inoue, Takahiro; Ichihara, Kenji; Ikari, Akira
Source:
Biochemical and biophysical research communications 2018 v.497 no.1 pp. 437-443
ISSN:
0006-291X
Subject:
DNA fragmentation; antineoplastic activity; antineoplastic agents; apoptosis; autophagy; caffeic acid; caspase-3; cell viability; chlorogenic acid; chloroquine; cinnamic acid; cytotoxicity; ethanol; honey bees; necroptosis; neoplasm cells; p-coumaric acid; propolis; prostatic neoplasms; protein-serine-threonine kinases
Abstract:
... Propolis, a resinous substance produced by honeybees, possesses various biological actions including anticancer activity towards tumor cells. Recently, the ethanol extract of Brazilian green propolis has been shown to induce autophagy, which is known to be induced in treatment of cancer cells with anticancer drugs, leading to cancer cell survival and decreased sensitivity to anticancer agents. In ...
DOI:
10.1016/j.bbrc.2018.02.105

11. The photodynamic action of pheophorbide a induces cell death through oxidative stress in Leishmania amazonensis

Author:
Miranda, Nathielle; Volpato, Hélito; da Silva Rodrigues, Jean Henrique; Caetano, Wilker; Ueda-Nakamura, Tânia; de Oliveira Silva, Sueli; Nakamura, Celso Vataru
Source:
Journal of photochemistry and photobiology 2017 v.174 pp. 342-354
ISSN:
1011-1344
Subject:
DNA fragmentation; Leishmania amazonensis; adverse effects; amastigotes; antioxidant activity; apoptosis; autophagy; droplets; leishmaniasis; lipid peroxidation; mechanism of action; membrane potential; mitochondrial membrane; new drugs; oxidative stress; parasites; patients; phosphatidylserines; photochemotherapy; photosensitizing agents; singlet oxygen; vacuoles
Abstract:
... Leishmaniasis is a disease caused by hemoflagellate protozoa, affecting millions of people worldwide. The difficulties of treating patients with this parasitosis include the limited efficacy and many side effects of the currently available drugs. Therefore, the search for new compounds with leishmanicidal action is necessary. Photodynamic therapy has been studied in the medical field because of it ...
DOI:
10.1016/j.jphotobiol.2017.08.016

12. Quercetin simultaneously induces G0/G1‐phase arrest and caspase‐mediated crosstalk between apoptosis and autophagy in human leukemia HL‐60 cells

Author:
Chang, Junn‐Liang; Chow, Jyh‐Ming; Chang, Jer‐Hwa; Wen, Yu‐Ching; Lin, Yung‐Wei; Yang, Shun‐Fa; Lee, Wei‐Jiunn; Chien, Ming‐Hsien
Source:
Environmental toxicology 2017 v.32 no.7 pp. 1857-1868
ISSN:
1520-4081
Subject:
DNA fragmentation; adenosine diphosphate; antineoplastic activity; apoptosis; autophagy; caspases; cell proliferation; cyclin-dependent kinase; enzyme inhibitors; humans; interphase; myeloid leukemia; organelles; quercetin; ribose
Abstract:
... Quercetin is a plant‐derived bioflavonoid with high anticancer activity in various tumors. Herein, the molecular mechanisms by which quercetin exerts its anticancer effects against HL‐60 acute myeloid leukemia (AML) cells were investigated. Results showed that quercetin suppressed cell proliferation in the HL‐60 cell line in vitro and in vivo. Quercetin‐induced G₀/G₁‐phase arrest occurred when exp ...
DOI:
10.1002/tox.22408

13. Inhibition of ROS production, autophagy or apoptosis signaling reversed the anticancer properties of Antrodia salmonea in triple-negative breast cancer (MDA-MB-231) cells

Author:
Chang, Chia-Ting; Korivi, Mallikarjuna; Huang, Hui-Chi; Thiyagarajan, Varadharajan; Lin, Kai-Yuan; Huang, Pei-Jane; Liu, Jer-Yuh; Hseu, You-Cheng; Yang, Hsin-Ling
Source:
Food and chemical toxicology 2017 v.103 pp. 1-17
ISSN:
0278-6915
Subject:
Antrodia; DNA; DNA fragmentation; acetylcysteine; antineoplastic activity; apoptosis; autophagy; bioluminescence; breast neoplasms; cell viability; culture media; death; humans; image analysis; medicinal fungi; mice; mitochondria; necrosis; signal transduction; toxicology; Taiwan
Abstract:
... We investigated the in vitro and in vivo anticancer properties of Antrodia salmonea (AS), a well-known edible/medicinal mushroom in Taiwan, on human triple-negative breast cancer (MDA-MB-231) cells and xenografted nude mice; and revealed the underlying molecular mechanisms involved in autophagic- and apoptotic-cell death. Treatment of MDA-MB-231 cells with fermented culture broth of AS (0–200 μg/m ...
DOI:
10.1016/j.fct.2017.02.019

14. Inhibition of autophagy enhances dynamin inhibitor-induced apoptosis via promoting Bak activation and mitochondrial damage in human Jurkat T cells

Author:
Hee Kim, Yoon; Kim, Ki Yun; Jun, Do Youn; Kim, Jong-Sik; Kim, Young Ho
Source:
Biochemical and biophysical research communications 2016 v.478 pp. 1609-1616
ISSN:
0006-291X
Subject:
DNA fragmentation; T-lymphocytes; antineoplastic activity; apoptosis; autophagy; bromides; caspase-9; cytokinesis; dynamins; gene expression regulation; gene overexpression; humans; membrane potential; mitochondria; mitochondrial membrane; necrosis
Abstract:
... Treatment of Jurkat T cells with the dynamin inhibitor, myristyl trimethyl ammonium bromides (MiTMAB) caused cytokinesis impairment and apoptotic DNA fragmentation along with down-regulation of anti-apoptotic BAG3 and Mcl-1 levels, Bak activation, mitochondrial membrane potential (Δψm) loss, activation of caspase-9 and -3, and PARP cleavage, without accompanying necrosis. Bcl-xL overexpression com ...
DOI:
10.1016/j.bbrc.2016.08.165

15. Enhanced autophagic activity of artocarpin in human hepatocellular carcinoma cells through improving its solubility by a nanoparticle system

Author:
Tzeng, Cheng-Wei; Tzeng, Wen-Sheng; Lin, Liang-Tzung; Lee, Chiang-Wen; Yen, Feng-Lin; Lin, Chun-Ching
Source:
Phytomedicine 2016 v.23 no.5 pp. 528-540
ISSN:
0944-7113
Subject:
Artocarpus altilis; DNA fragmentation; acridine orange; antineoplastic activity; apoptosis; autophagy; cell cycle; chemoprevention; cytotoxicity; drug therapy; encapsulation; energy; flavonoids; hepatoma; humans; hydrogen bonding; nanoparticles; neoplasm cells; particle size; prognosis; staining; water solubility
Abstract:
... Hepatocellular carcinoma (HCC) is the most common liver cancer worldwide, with poor prognosis and resistance to chemotherapy. This gives novel cancer treatment methods an overwhelming significance. Natural products offer great resources of developing new and effective chemopreventive or chemotherapeutic agents. Artocarpus communis extracts and its active constituent, prenylated flavonoid artocarpi ...
DOI:
10.1016/j.phymed.2016.02.010

16. Excessive apoptosis and defective autophagy contribute to developmental testicular toxicity induced by fluoride

Author:
Zhang, Shun; Niu, Qiang; Gao, Hui; Ma, Rulin; Lei, Rongrong; Zhang, Cheng; Xia, Tao; Li, Pei; Xu, Chunyan; Wang, Chao; Chen, Jingwen; Dong, Lixing; Zhao, Qian; Wang, Aiguo
Source:
Environmental pollution 2016 v.212 pp. 97-104
ISSN:
0269-7491
Subject:
DNA fragmentation; animal models; apoptosis; autophagy; caspase-3; chromatin; cytochrome c; death; drinking water; germ cells; histopathology; humans; males; progeny; rats; reproductive toxicology; sodium fluoride; testes; toxicity
Abstract:
... Fluoride, a ubiquitous environmental contaminant, is known to impair testicular functions and fertility; however the underlying mechanisms remain obscure. In this study, we used a rat model to mimic human exposure and sought to investigate the roles of apoptosis and autophagy in testicular toxicity of fluoride. Sprague–Dawley rats were developmentally exposed to 25, 50, or 100 mg/L sodium fluoride ...
DOI:
10.1016/j.envpol.2016.01.059

17. Tetrandrine induces apoptosis Via caspase‐8, ‐9, and ‐3 and poly (ADP ribose) polymerase dependent pathways and autophagy through beclin‐1/ LC3‐I, II signaling pathways in human oral cancer HSC‐3 cells

Author:
Yu, Fu‐Shun; Yu, Chun‐Shu; Chen, Jaw‐Chyun; Yang, Jiun‐Long; Lu, Hsu‐Feng; Chang, Shu‐Jen; Lin, Meng‐Wei; Chung, Jing‐Gung
Source:
Environmental toxicology 2016 v.31 no.4 pp. 395-406
ISSN:
1520-4081
Subject:
DNA fragmentation; Stephania tetrandra; adenosine diphosphate; alkaloids; apoptosis; autophagy; caspase-8; cell viability; chromatin; cytotoxicity; humans; mouth neoplasms; neoplasm cells; ribose; signal transduction; squamous cell carcinoma
Abstract:
... Tetrandrine is a bisbenzylisoquinoline alkaloid that was found in the Radix Stephania tetrandra S Moore. It had been reported to induce cytotoxic effects on many human cancer cells. In this study, we investigated the cytotoxic effects of tetrandrine on human oral cancer HSC‐3 cells in vitro. Treatments of HSC‐3 cells with tetrandrine significantly decreased the percentage of viable cells through t ...
DOI:
10.1002/tox.22053

18. Blue light emitting diode induces apoptosis in lymphoid cells by stimulating autophagy

Author:
Oh, Phil-Sun; Hwang, Hyosook; Jeong, Hwan-Seok; Kwon, Jeongil; Kim, Hyun-Soo; Kim, Minjoo; Lim, SeokTae; Sohn, Myung-Hee; Jeong, Hwan-Jeong
Source:
The international journal of biochemistry & cell biology 2016 v.70 pp. 13-22
ISSN:
1357-2725
Subject:
DNA fragmentation; apoptosis; autophagy; blue light; caspase-3; cell viability; irradiation; lymph; membrane potential; mice; mitochondrial membrane; neoplasm cells; oxygen; phosphatidylserines; proteins; small interfering RNA; staining; superoxide anion; survival rate; transfection
Abstract:
... The present study was performed to examine the induction of apoptotic cell death and autophagy by blue LED irradiation, and the contribution of autophagy to apoptosis in B cell lymphoma A20 and RAMOS cells exposed to blue LED. Irradiation with blue LED reduced cell viability and induced apoptotic cell death, as indicated by exposure of phosphatidylserine on the plasma outside membrane and fragment ...
DOI:
10.1016/j.biocel.2015.11.004

19. Studying Different Binding and Intracellular Delivery Efficiency of ssDNA Single-Walled Carbon Nanotubes and Their Effects on LC3-Related Autophagy in Renal Mesangial Cells via miRNA-382

Author:
Wang, Guobao; Zhao, Tingting; Wang, Leyu; Hu, Bianxiang; Darabi, Ali; Lin, Jiansheng; Xing, Malcolm M. Q.; Qiu, Xiaozhong
Source:
ACS Applied Materials & Interfaces 2015 v.7 no.46 pp. 25733-25740
ISSN:
1944-8252
Subject:
DNA fragmentation; apoptosis; autophagy; carbon nanotubes; coculture; fibrosis; microRNA; necrosis; oligonucleotides; single-stranded DNA
Abstract:
... Single-walled carbon nanotubes (SWCNTs) have been used to deliver single-stranded (ssDNA). ssDNA in oligonucleotide can act as an inhibitor of microRNA to regulate cellular functions. However, these ssDNA are difficult to bind carbon nanotubes with low transferring efficiency to cells. To this end, we designed ssDNA with regulatory and functional units to form ssDNA-SWCNT hybrids to study their bi ...
DOI:
10.1021/acsami.5b07185

20. Omega-3 DHA- and EPA–dopamine conjugates induce PPARγ-dependent breast cancer cell death through autophagy and apoptosis

Author:
Rovito, Daniela; Giordano, Cinzia; Plastina, Pierluigi; Barone, Ines; De Amicis, Francesca; Mauro, Loredana; Rizza, Pietro; Lanzino, Marilena; Catalano, Stefania; Bonofiglio, Daniela; Andò, Sebastiano
Source:
BBA - General Subjects 2015 v.1850 pp. 2185-2195
ISSN:
0304-4165
Subject:
DNA damage; DNA fragmentation; RNA; adjuvants; amines; antagonists; apoptosis; autophagy; bioactive properties; breast neoplasms; chromatin; docosahexaenoic acid; eicosapentaenoic acid; epithelium; immunoblotting; neoplasm cells; precipitin tests; viability
Abstract:
... The omega-3 docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) may form conjugates with amines that have potential health benefits against common diseases including cancers. Here we synthesized DHA-dopamine (DHADA) and EPA–dopamine (EPADA) conjugates and studied their biological effects on different breast cancer cell lines.MTT assays indicated that increasing concentrations of DHADA and E ...
DOI:
10.1016/j.bbagen.2015.08.004

21. Melipona quadrifasciata (Hymenoptera: Apidae) fat body persists through metamorphosis with a few apoptotic cells and an increased autophagy

Author:
Santos, Douglas Elias; Azevedo, Dihego Oliveira; Campos, Lúcio Antônio Oliveira; Zanuncio, José Cola; Serrão, José Eduardo
Source:
Protoplasma 2015 v.252 no.2 pp. 619-627
ISSN:
0033-183X
Subject:
DNA; DNA fragmentation; Melipona quadrifasciata; apoptosis; autophagy; caspase-3; energy; fat body; genes; imagos; larvae; messenger RNA; metamorphosis; mitochondria; pupae; recycling; reverse transcriptase polymerase chain reaction; ribosomes; stingless bees
Abstract:
... Fat body, typically comprising trophocytes, provides energy during metamorphosis. The fat body can be renewed once the larval phase is complete or recycled and relocated to form the fat body of the adult insect. This study aims to identify the class of programmed cell death that occurs within the fat body cells during the metamorphosis of the stingless bee Melipona quadrifasciata. Using immunodete ...
DOI:
10.1007/s00709-014-0707-z

22. Phellinus Linteus Extract Induces Autophagy and Synergizes With 5-Fluorouracil to Inhibit Breast Cancer Cell Growth

Author:
Lee, Wen-Ying; Hsu, Keng-Fu; Chiang, Tai-An; Chen, Chee-Jen
Source:
Nutrition and cancer 2015 v.67 no.2 pp. 275-284
ISSN:
1532-7914
Subject:
DNA fragmentation; Phellinus linteus; anticarcinogenic activity; apoptosis; autophagy; breast neoplasms; cell growth; medicinal fungi; nutrition; propidium; transmission electron microscopy; vacuoles
Abstract:
... Phellinus linteus (PL) is a medicinal mushroom due to its several biological properties, including anticancer activity. However, the mechanisms of its anticancer effect remain to be elucidated. We evaluated the inhibitory effects of the ethanolic extract from the PL combined with 5-FU on MDA-MB-231 breast cancer cell line and to determine the mechanism of cell death. Individually, PL extract and 5 ...
DOI:
10.1080/01635581.2015.989374

23. Upregulation of Atg5 and AIF gene expression in synchronization with programmed cellular death events in integumental epithelium of Bombyx mori induced by a dipteran parasitoid infection

Author:
Anitha, J.; Pradeep, A.R.; Sivaprasad, V.
Source:
Bulletin of entomological research 2014 v.104 no.6 pp. 794-800
ISSN:
1475-2670
Subject:
Bombyx mori; DNA; DNA fragmentation; Exorista; apoptosis; autophagy; caspases; endoplasmic reticulum; epithelial cells; epithelium; gene expression; gene expression regulation; genes; invertebrates; larvae; mass spectrometry; parasitoids; proteins; silkworms
Abstract:
... Infection of the commercially important silkworm, Bombyx mori by a tachnid parasitoid, Exorista bombycis induced activation of genes and cellular responses associated with apoptosis in integumental epithelial cells. Composite cellular profile showed initial autophagy, intermediate endoplasmic reticulum degranulation and deformed nucleus as well as later DNA fragmentation indicating apoptosis. Two ...
DOI:
10.1017/S0007485314000686
PubMed:
25246086

24. Cell death and tissue reorganization in Rhynchosciara americana (Sciaridae: Diptera) metamorphosis and their relation to molting hormone titers

Author:
Brandão, Amanda dos Santos; do Amaral, Jônatas Bussador; Rezende-Teixeira, Paula; Hartfelder, Klaus; Siviero, Fábio; Machado-Santelli, Gláucia Maria
Source:
Arthropod structure & development 2014 v.43 no.5 pp. 511-522
ISSN:
1467-8039
Subject:
DNA fragmentation; Rhynchosciara americana; adults; apoptosis; autophagy; chromatin; confocal microscopy; death; ecdysone; fat body; genes; insects; metamorphosis; molting; morphogenesis; pupae; salivary glands; transmission electron microscopy; vacuoles
Abstract:
... Programmed cell death (PCD) is a focal topic for understanding processes underlying metamorphosis in insects, especially so in holometabolous orders. During adult morphogenesis it allows for the elimination of larva-specific tissues and the reorganization of others for their functionalities in adult life. In Rhynchosciara, this PCD process could be classified as autophagic cell death, yet the expr ...
DOI:
10.1016/j.asd.2014.05.001

25. Reduced scytonemin isolated from Nostoc commune induces autophagic cell death in human T-lymphoid cell line Jurkat cells

Author:
Itoh, Tomohiro; Tsuzuki, Ryosuke; Tanaka, Toshiomi; Ninomiya, Masayuki; Yamaguchi, Yuji; Takenaka, Hiroyuki; Ando, Masashi; Tsukamasa, Yasuyuki; Koketsu, Mamoru
Source:
Food and chemical toxicology 2013 v.60 pp. 76-82
ISSN:
0278-6915
Subject:
DNA fragmentation; Nostoc commune; acetylcysteine; apoptosis; aquatic environment; autophagy; cell growth; growth retardation; humans; reactive oxygen species; soil; vacuoles
Abstract:
... Nostoc commune is a terrestrial benthic blue-green alga that often forms an extended mucilaginous layer on the soil, accumulates on stones and mud in aquatic environments. Reduced-scytonemin (R-scy), isolated from N. commune Vaucher, has been shown to suppress the human T-lymphoid Jurkat cell growth. To reveal the mechanisms underlying the R-scy-mediated inhibition of Jurkat cell growth, we examin ...
DOI:
10.1016/j.fct.2013.07.016

26. Dual Role of Dextran Sulfate 5000 Da as Anti-Apoptotic and Pro-Autophagy Agent

Author:
Menvielle, J. Pedro; Safini, Najete; Tisminetzky, Sergio G.; Skoko, Natasa
Source:
Molecular biotechnology 2013 v.54 no.2 pp. 711-720
ISSN:
1073-6085
Subject:
Chinese hamsters; DNA fragmentation; animal ovaries; apoptosis; autophagy; cell culture; cell cycle; cell viability; dextran; erythropoietin
Abstract:
... Dextran sulfate 5,000 Da (DS), a sulfated polysaccharide, has been used in recombinant mammalian cell cultures to prevent cell aggregation, thereby increasing cell viability. Previous studies using Chinese hamster ovary (CHO) suspension cultures had shown that low concentrations of DS are related to an inhibition of apoptosis. In this study, DS was used on anchorage-dependent CHO cells producing e ...
DOI:
10.1007/s12033-012-9620-x
PubMed:
23180219

27. Activation of autophagic programmed cell death and innate immune gene expression reveals immuno-competence of integumental epithelium in Bombyx mori infected by a dipteran parasitoid

Author:
Pradeep, Appukuttan Nair R.; Anitha, Jayaram; Awasthi, Arvind K.; Babu, Mohd. A.; Geetha, Murthy N.; Arun, Hariharan K.; Chandrashekhar, Sagar; Rao, Guruprasad C.; Vijayaprakash, Nanjappa B.
Source:
Cell and tissue research 2013 v.352 no.2 pp. 371-385
ISSN:
0302-766X
Subject:
Bombyx mori; DNA fragmentation; Exorista; apoptosis; autophagy; beta-N-acetylhexosaminidase; caspases; chitinase; cytokines; cytotoxicity; enzyme activity; epithelium; gene expression; gene expression regulation; genes; humoral immunity; immune response; integument; melanin; melanization; metabolism; molecular chaperones; monophenol monooxygenase; parasitoids; prophenoloxidase; proteolysis; proteomics; quantitative polymerase chain reaction; quinones; silkworms; transcription factors; tyrosine; vacuoles
Abstract:
... In insects, the integument forms the primary barrier between the environment and internal milieu, but cellular and immune responses of the integumental epithelium to infection by micro- and macro-parasites are mostly unknown. We elucidated cellular and immune responses of the epithelium induced through infection by a dipteran endoparasitoid, Exorista bombycis in the economically important silkworm ...
DOI:
10.1007/s00441-012-1520-7
PubMed:
23161099

28. Cell death mechanisms during follicular atresia in Dipetalogaster maxima, a vector of Chagas’ disease (Hemiptera: Reduviidae)

Author:
Aguirre, Silvina A.; Pons, Patricia; Settembrini, Beatriz P.; Arroyo, Daniela; Canavoso, Lilián E.
Source:
Journal of insect physiology 2013 v.59 no.5 pp. 532-541
ISSN:
0022-1910
Subject:
follicular atresia; acid phosphatase; DNA fragmentation; necrosis; insect rearing; blood meal; apoptosis; insect vectors; autophagy; transmission electron microscopy; abnormal development; chromatin; hematophagous insects; fluorescent antibody technique; animal ovaries; females; Dipetalogaster; caspase-3
Abstract:
... In this work we have analyzed the involvement of cell death pathways during the process of follicular atresia in the hematophagous insect vector Dipetalogaster maxima. Standardized insect rearing conditions were established to induce a gradual follicular degeneration stage by depriving females of blood meal during post-vitellogenesis. We first characterized the morpho-histological and ultrastructu ...
DOI:
10.1016/j.jinsphys.2013.03.001
PubMed:
23500893

29. Graphene quantum dots as autophagy-inducing photodynamic agents

Author:
Markovic, Zoran M.; Ristic, Biljana Z.; Arsikin, Katarina M.; Klisic, Djordje G.; Harhaji-Trajkovic, Ljubica M.; Todorovic-Markovic, Biljana M.; Kepic, Dejan P.; Kravic-Stevovic, Tamara K.; Jovanovic, Svetlana P.; Milenkovic, Marina M.; Milivojevic, Dusan D.; Bumbasirevic, Vladimir Z.; Dramicanin, Miroslav D.; Trajkovic, Vladimir S.
Source:
Biomaterials 2012 v.33 no.29 pp. 7084-7092
ISSN:
0142-9612
Subject:
DNA fragmentation; apoptosis; autophagy; blue light; caspases; cytotoxicity; electrochemistry; graphene; humans; oxidative stress; phosphatidylserines; photoluminescence; quantum dots; singlet oxygen; therapeutics
Abstract:
... The excellent photoluminescent properties of graphene quantum dots (GQD) makes them suitable candidates for biomedical applications, but their cytotoxicity has not been extensively studied. Here we show that electrochemically produced GQD irradiated with blue light (470nm, 1W) generate reactive oxygen species, including singlet oxygen, and kill U251 human glioma cells by causing oxidative stress. ...
DOI:
10.1016/j.biomaterials.2012.06.060

30. Synergistic killing effect of chloroquine and androgen deprivation in LNCaP cells

Author:
Kaini, Ramesh R.; Hu, Chien-An A.
Source:
Biochemical and biophysical research communications 2012 v.425 no.2 pp. 150-156
ISSN:
0006-291X
Subject:
death; DNA fragmentation; therapeutics; adenosine triphosphate; prostatic neoplasms; chloroquine; droplets; apoptosis; autophagy; protective effect
Abstract:
... Modulation of autophagy is a new paradigm in cancer therapeutics. Recently a novel function of chloroquine (CLQ) in inhibiting degradation of autophagic vesicles has been revealed, which raises the question whether CLQ can be used as an adjuvant in targeting autophagic pro-survival mechanism in prostate cancer (PCa). We previously showed that autophagy played a protective role during hormone ablat ...
DOI:
10.1016/j.bbrc.2012.07.054

31. Autophagy is induced by the type III secretion system of Vibrio alginolyticus in several mammalian cell lines

Author:
Zhao, Zhe; Zhang, Lvping; Ren, Chunhua; Zhao, Jingjing; Chen, Chang; Jiang, Xiao; Luo, Peng; Hu, Chao-Qun
Source:
Archives of microbiology 2011 v.193 no.1 pp. 53-61
ISSN:
0302-8933
Subject:
DNA fragmentation; Gram-negative bacteria; Vibrio alginolyticus; Western blotting; apoptosis; autophagy; caspase-1; caspase-3; cytolysis; fish; fluorescence; humans; pathogens; type III secretion system
Abstract:
... Vibrio alginolyticus is a gram-negative bacterium and has been recognized as an opportunistic pathogen in marine animals as well as humans. Here, we further characterized a cell death mechanism caused by this bacterium in several mammalian cell lines. The T3SS of V. alginolyticus killed HeLa cells by a very similar cell cytolysis mechanism in fish cells, as evidenced by cell rounding and LDH relea ...
DOI:
10.1007/s00203-010-0646-9

32. Skn1 and Ipt1 negatively regulate autophagy in Saccharomyces cerevisiae

Author:
Thevissen, Karin; Yen, Wei-Lien; Carmona-Gutierrez, Didac; Idkowiak-Baldys, Jolanta; Aerts, An M.; François, Isabelle E.J.A.; Madeo, Frank; Klionsky, Daniel J.; Hannun, Yusuf A.; Cammue, Bruno P.A.
Source:
FEMS microbiology letters 2010 v.303 no.2 pp. 163-168
ISSN:
0378-1097
Subject:
DNA fragmentation; Saccharomyces cerevisiae; apoptosis; autophagy; biosynthesis; ceramides; mutants; nitrogen; proteins; starvation; yeasts
Abstract:
... We demonstrated that a yeast deletion mutant in IPT1 and SKN1, encoding proteins involved in the biosynthesis of mannosyldiinositolphosphoryl ceramides, is characterized by increased autophagy and DNA fragmentation upon nitrogen (N) starvation as compared with the single deletion mutants or wild type (WT). Apoptotic features were not significantly different between single and double deletion mutan ...
DOI:
10.1111/j.1574-6968.2009.01869.x
PubMed:
20030721
PubMed Central:
PMC2818745

33. Modes of cell death in the pupal perivisceral fat body tissue of the silkworm Bombyx mori L

Author:
Sumithra, Purushothaman; Britto, Cathrin P.; Krishnan, Muthukalingan
Source:
Cell and tissue research 2010 v.339 no.2 pp. 349-358
ISSN:
0302-766X
Subject:
DNA fragmentation; acid phosphatase; metamorphosis; acridine orange; silkworms; DNA; proteolysis; vacuoles; fat body; autophagy; animal development; Bombyx mori; mitochondria; chromatin; scanning electron microscopy; apoptosis
Abstract:
... Cell death is a scheduled event during animal development and tissue turnover. Here, we affirm the presence of two major pathways of programmed cell death (PCD), viz. apoptotic and autophagic cell death, in the disintegrated pupal perivisceral (PV) fat body during pupal-adult metamorphosis. The acridine orange (a vital stain for apoptosis) staining pattern and DNA fragmentation assay have revealed ...
DOI:
10.1007/s00441-009-0898-3
PubMed:
19949813

34. Amino Acid Deprivation Induces Both Apoptosis and Autophagy in Murine C2C12 Muscle Cells

Author:
Martinet, Wim; De Meyer, Guido R.Y.; Herman, Arnold G.; Kockx, Mark M.
Source:
Biotechnology letters 2005 v.27 no.16 pp. 1157-1163
ISSN:
0141-5492
Subject:
DNA fragmentation; amino acids; apoptosis; autophagy; blood serum; caspase-3; caspase-8; chromatin; culture media; cycloheximide; mice; myocytes; pro-apoptotic proteins; starvation; tumor necrosis factor-alpha
Abstract:
... Apoptosis and autophagy are closely interconnected types of programmed cell death. In the present study, mouse C2C12 muscle cells were starved in Earle’s Balanced Salt Solution or treated with TNF-α and cycloheximide to induce autophagy and apoptosis, respectively. The majority of starved C2C12 cells underwent autophagy, as shown by LC3 processing, formation of autophagic vesicles and bulk degrada ...
DOI:
10.1007/s10529-005-0007-y