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You searched for: Subject term DNA primers Remove constraint Subject term: DNA primers Text availability Full Text Remove constraint Text availability: Full Text
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1. A Broad-Host-Range, Generalized Transducing Phage (SN-T) Acquires 16S rRNA Genes from Different Genera of Bacteria

Author:
Beumer, Amy; Robinson, Jayne B.
Source:
Applied and environmental microbiology 2005 v.71 no.12 pp. 8301-8304
ISSN:
0099-2240
Subject:
DNA primers; Pseudomonas aeruginosa; Sphaerotilus natans; bacteria; bacteriophages; gene transfer; genes; genomics; nucleotide sequences; polymerase chain reaction; restriction fragment length polymorphism; ribosomal RNA
Abstract:
... Genomic analysis has revealed heterogeneity among bacterial 16S rRNA gene sequences within a single species; yet the cause(s) remains uncertain. Generalized transducing bacteriophages have recently gained recognition for their abundance as well as their ability to affect lateral gene transfer and to harbor bacterial 16S rRNA gene sequences. Here, we demonstrate the ability of broad-host-range, gen ...
DOI:
10.1128/AEM.71.12.8301-8304.2005
PubMed:
16332816
PubMed Central:
PMC1317401

2. A Combination of Real-Time PCR and High-Resolution Melting Analysis to Detect and Identify CpGV Genotypes Involved in Type I Resistance

Author:
Aurélie Hinsberger; Stéphane Theulier Saint Germain; Patrice Guerrero; Christine Blachère-López; Miguel López-Ferber; Sandrine Bayle
Source:
Viruses 2019 v.11 no.8 pp. -
ISSN:
1999-4915
Subject:
Cydia pomonella; Cydia pomonella granulovirus; DNA primers; biological control; genes; genotype; insect control; larvae; melting; melting point; orchards; quantitative polymerase chain reaction; rapid methods
Abstract:
... Cydia pomonella granulovirus, in particular CpGV-M isolate, is used as a biological control against the codling moth (CM), Cydia pomonella. As a result of intensive control over the years, codling moth populations have developed resistance against this isolate. This resistance is now called type I resistance. Isolates, among them, CpGV-R5, have been found that are able to overcome type I resistanc ...
DOI:
10.3390/v11080723
PubMed:
31390849
PubMed Central:
PMC6723291

3. A Comparative Study of Three Detection Techniques for Leifsonia xyli Subsp. xyli, the Causal Pathogen of Sugarcane Ratoon Stunting Disease Subsp. the Causal Pathogen of Sugarcane Ratoon

Author:
Qibin Wu; Yong-Bao Pan; Dinggang Zhou; Michael Grisham P.; Shiwu Gao; Yachun Su; Jinlong Guo; Liping Xu; Youxiong Que
Source:
BioMed research international 2018 v.2018 no. pp. -
ISSN:
2314-6141
Subject:
DNA primers; Leifsonia xyli subsp. xyli; Saccharum; comparative study; detection limit; disease detection; loop-mediated isothermal amplification; microbial detection; plant pathogenic bacteria; quantitative polymerase chain reaction; ratoon stunting disease; stems; sugarcane juice
Abstract:
... The ratoon stunting disease (RSD), caused by the bacterium Leifsonia xyli subsp. xyli (Lxx), is one of the most economically devastating diseases impacting sugarcane. RSD causes significant yield losses and variety degradation. Diagnosis of RSD is challenging because it does not exhibit any discernible internal and external symptoms. Moreover, the Lxx bacteria are very small and difficult to isola ...
Handle:
10113/6740177
DOI:
10.1155/2018/2786458
PubMed:
29951532
PubMed Central:
PMC5989284

4. A DNA sequencing strategy that requires only five bases of known terminal sequence for priming

Author:
Fu, D.J.; Broude, N.E.; Koster, H.; Smith, C.L.; Cantor, C.R.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.22 pp. 10162-10166
ISSN:
0027-8424
Subject:
sequence analysis; DNA; DNA primers
Abstract:
... We have previously reported an enhanced version of sequencing by hybridization (SBH), termed positional SBH (PSBH). PSBH uses partially duplex probes containing single-stranded 3' overhangs, instead of simple single-stranded probes. Stacking interactions between the duplex probe and a single-stranded target allow us to reduce the probe sizes required to 5-base single-stranded overhangs. Here we de ...
DOI:
10.1073/pnas.92.22.10162
PubMed:
7479746
PubMed Central:
PMC40756

5. A DNA-based method for detection of Peridermium harknessii, the causal agent of western gall rust

Author:
Ramsfield, Tod D.; Vogler, Detlev R.
Source:
Australasian plant pathology 2010 v.39 no.3 pp. 247-253
ISSN:
0815-3191
Subject:
Endocronartium harknessii; rust diseases; biosecurity; DNA primers; Cronartium; polymerase chain reaction; phylogeny; tree diseases; microbial detection; invasive species; disease diagnosis
Abstract:
... Peridermium harknessii is not present in the southern hemisphere yet it poses a serious threat to Pinus radiata cultivation in exotic forest plantations there. If a suspected incursion were to occur, it would be necessary to rapidly confirm the presence of P. harknessii within non-sporulating galls. With this in mind, we have developed a DNA-based identification system, using the first intergenic ...
Handle:
10113/42655
DOI:
10.1071/AP09058

6. A Method for Molecular Analysis of Catalase Gene Diversity in Seawater

Author:
Wei Wang; Xiaofeng Ji; Cui Yuan; Fangqun Dai; Jiancheng Zhu; Mi Sun
Source:
Indian journal of microbiology 2013 v.53 no.4 pp. 477-481
ISSN:
0046-8991
Subject:
DNA; DNA libraries; DNA primers; Escherichia coli; agarose; bacteria; catalase; gel electrophoresis; genes; marine environment; metabolism; metagenomics; polymerase chain reaction; restriction endonucleases; restriction fragment length polymorphism; seawater; surface water; Yellow Sea
Abstract:
... Catalase plays an important role in the metabolism of marine bacteria and has potential impact on the marine environment. Four PCR primers were designed to amplify the catalase gene fragments in marine bacteria by applying metagenomic DNA from Yellow Sea surface water as the template. Of the four reproducible target PCR products, the longest one with 900 bp were chosen for catalase gene library co ...
DOI:
10.1007/s12088-013-0404-1
PubMed:
24426153
PubMed Central:
PMC3779292

7. A New Rapid Methodological Strategy to Assess BRCA Mutational Status

Author:
Emilia Vuttariello; Marco Borra; Celeste Calise; Elvira Mauriello; Stefano Greggi; Aldo Vecchione; Elio Biffali; Gennaro Chiappetta
Source:
Molecular biotechnology 2013 v.54 no.3 pp. 954-960
ISSN:
1073-6085
Subject:
DNA primers; DNA repair; animal ovaries; breast neoplasms; cost effectiveness; genes; mutation; ovarian neoplasms; patients; polymerase chain reaction; proteins; screening; therapeutics; women
Abstract:
... Hereditary cancers account for approximately 10 % of breast and ovarian cancers. Mutations of the BRCA1 and BRCA2 genes, encoding two proteins involved in DNA repair, underlie most cases of such hereditary cancers. Women with BRCA mutations develop breast cancer in 50–80 % of cases and ovarian cancer in 10–40 % of cases. Assessing BRCA mutational status is needed to direct the clinical management ...
DOI:
10.1007/s12033-012-9646-0
PubMed:
23354980
PubMed Central:
PMC3641296

8. A PCR protocol for the identification of Pseudomonas syringae pv. tagetis based on genes required for tagetitoxin production

Author:
Kong, H.; Patterson, C.D.; Zhang, W.; Takikawa, Y.; Suzuki, A.; Lydon, J.
Source:
Biological control 2004 v.30 no.1 pp. 83-89
ISSN:
1049-9644
Subject:
Pseudomonas syringae pv. tagetis; plant pathogenic bacteria; pathogen identification; polymerase chain reaction; genes; bacterial toxins; DNA primers; toxigenic strains; nontoxigenic strains; molecular systematics; Pseudomonas syringae pv. pisi; biological control agents; weed control; Cirsium arvense
Abstract:
... A polymerase chain reaction (PCR) protocol that can be used to distinguish Pseudomonas syringae pv. tagetis from other P. syringae pathovars, including those that induce apical chlorosis in several plants of the Asteracaea family and in pea, and closely related P. savastanoi pathovars was developed based on DNA sequences from P. syringae pv. tagetis that are required for tagetitoxin synthesis. PCR ...
Handle:
10113/9836

9. A PCR-based method for the detection of Ophiosphaerella agrostis in creeping bentgrass

Author:
Kaminski, J.E.; Dernoeden, P.H.; O'Neill, N.R.; Wetzel, H.C. III
Source:
Plant disease 2005 v.89 no.9 pp. 980-985
ISSN:
0191-2917
Subject:
grasses; disease detection; seeds; ribosomal DNA; fungal diseases of plants; polymerase chain reaction; host plants; DNA primers; internal transcribed spacers; pathogen identification; Pleosporales; plant pathogenic fungi; Agrostis stolonifera
Abstract:
... Dead spot is a relatively new disease of creeping bentgrass and hybrid bermudagrass that is incited by Ophiosphaerella agrostis. Initial symptoms are difficult to diagnose and clinicians generally rely on the presence of pseudothecia within infected tissue or isolation of O. agrostis on an artificial medium. The main goal of this study was to develop a polymerase chain reactionbased technique capa ...
Handle:
10113/181
DOI:
10.1094/PD-89-0980

10. A Phytoplasma Related to 'Candidatus Phytoplasma asteri' Detected in Citrus Showing Huanglongbing (Yellow Shoot Disease) Symptoms in Guangdong, P. R. China

Author:
Chen, J.; Pu, X.; Deng, X.; Liu, S.; Li, H.; Civerolo, E.
Source:
Phytopathology 2009 v.99 no.3 pp. 236-242
ISSN:
0031-949X
Subject:
Phytoplasma; greening disease; phytoplasmal diseases; Candidatus Liberibacter; strains; signs and symptoms (plants); polymerase chain reaction; nucleotide sequences; DNA primers; sequence analysis; disease detection; Catharanthus roseus; Citrus reticulata; Citrus sinensis; Citrus maxima; correlation; China
Abstract:
... Citrus huanglongbing (HLB) or yellow shoot disease (i.e., greening disease) is highly destructive to citrus production worldwide. Understanding the etiology of HLB is critical for managing the disease. HLB is currently associated with infection by 'Candidatus Liberibacter spp.' around the world, including China. However, Koch's postulates have not been fulfilled. In addition, other plant pathogens ...
Handle:
10113/29150
DOI:
10.1094/PHYTO-99-3-0236
PubMed:
19203275

11. A Simple Allele-Specific PCR Assay for Detecting FAD2 Alleles in Both A and B Genomes of the Cultivated Peanut for High-Oleate Trait Selection

Author:
Chen, Zhenbang; Wang, Ming Li; Barkley, Noelle A.; Pittman, Roy N.
Source:
Plant molecular biology reporter 2010 v.28 no.3 pp. 542-548
ISSN:
0735-9640
Subject:
Arachis hypogaea; polymerase chain reaction; alleles; tetraploidy; oleic acid; stearoyl-CoA desaturase; cultivars; mutation; marker-assisted selection; gas chromatography; fatty acid composition; germplasm screening; DNA primers
Abstract:
... In cultivated tetraploid peanut (2n = 4x = 40, AABB), the conversion of oleic acid to linoleic acid is mainly catalyzed by the Δ¹² fatty acid desaturase (FAD). Two homoeologous genes (FAD2A and FAD2B) encoding for the desaturase are located on the A and B genomes, respectively. Abolishing or reducing the desaturase activity by gene mutation can significantly increase the oleic acid/linoleic acid r ...
Handle:
10113/42005
DOI:
10.1007/s11105-010-0181-5

12. A Universal Method for the Identification of Bacteria Based on General PCR Primers

Author:
Sameer A. Barghouthi
Source:
Indian journal of microbiology 2011 v.51 no.4 pp. 430-444
ISSN:
0046-8991
Subject:
DNA primers; Nematoda; bacteria; bacterial communities; blood; cerebrospinal fluid; cosmetics; databases; genes; microbial detection; polymerase chain reaction; ribosomal DNA; species identification
Abstract:
... The Universal Method (UM) described here will allow the detection of any bacterial rDNA leading to the identification of that bacterium. The method should allow prompt and accurate identification of bacteria. The principle of the method is simple; when a pure PCR product of the 16S gene is obtained, sequenced, and aligned against bacterial DNA data base, then the bacterium can be identified. Confi ...
DOI:
10.1007/s12088-011-0122-5
PubMed:
23024404
PubMed Central:
PMC3209952

13. A Y genome specific STS marker in Pseudoroegneria and Elymus species (Triticeae: Gramineae)

Author:
Okito, Pungu; Mott, Ivan W.; Wu, Yajun; Wang, Richard R.C.
Source:
Genome = 2009 v.52 no.4 pp. 391-400
ISSN:
0831-2796
Subject:
random amplified polymorphic DNA technique; sequence alignment; genome; DNA primers; Pseudoroegneria; Elymus; repetitive sequences; genetic markers
Abstract:
... The tribe Triticeae Dumortier in the grass family (Poaceae) includes the most important cereal crops (e.g., wheat, barley, and rye) and some economically important forage grasses. Elymus L. is the largest and most complex genus in the Triticeae tribe with approximately 150 species occurring worldwide. The genomic constitutions of approximately 40% of Elymus species are unknown and some have unveri ...
Handle:
10113/30449
DOI:
10.1139/g09-015
PubMed:
19370094

14. A comparative evaluation of PCR- based methods for species- specific determination of African animal trypanosomes in Ugandan cattle

Author:
Heba A Ahmed; Kim Picozzi; Susan C Welburn; Ewan T MacLeod
Source:
Parasites & vectors 2013 v.6 no.1 pp. 316
ISSN:
1756-3305
Subject:
DNA; DNA primers; Trypanosoma brucei; Trypanosoma congolense; Trypanosoma vivax; blood; bovine trypanosomiasis; cattle; epidemiological studies; genes; internal transcribed spacers; parasites; polymerase chain reaction; ribosomal RNA; screening; Uganda
Abstract:
... BACKGROUND: In recent years, PCR has been become widely applied for the detection of trypanosomes overcoming many of the constraints of parasitological and serological techniques, being highly sensitive and specific for trypanosome detection. Individual species-specific multi-copy trypanosome DNA sequences can be targeted to identify parasites. Highly conserved ribosomal RNA (rRNA) genes are also ...
DOI:
10.1186/1756-3305-6-316
PubMed:
24499678
PubMed Central:
PMC4029050

15. A comparative molecular and 3-dimensional structural investigation into cross-continental and novel avian Trypanosoma spp. in Australia

Author:
Crystal Cooper; R. C. Andrew Thompson; Adriana Botero; Amanda Kristancic; Christopher Peacock; Yaowanuj Kirilak; Peta L. Clode
Source:
Parasites & vectors 2017 v.10 no.1 pp. 234
ISSN:
1756-3305
Subject:
DNA primers; Hirudinea; Trypanosoma; binding proteins; birds; epimastigotes; evolution; genotype; kinetoplast DNA; mammals; mitochondria; models; polymerase chain reaction; scanning electron microscopy; Western Australia
Abstract:
... BACKGROUND: Molecular and structural information on avian Trypanosoma spp. throughout Australia is limited despite their intrinsic value in understanding trypanosomatid evolution, diversity, and structural biology. In Western Australia tissue samples (n = 429) extracted from 93 birds in 25 bird species were screened using generic PCR primers to investigate the diversity of Trypanosoma spp. To inve ...
DOI:
10.1186/s13071-017-2173-x
PubMed:
28499405
PubMed Central:
PMC5427604

16. A comprehensive screen for chicken proteins that interact with proteins unique to virulent strains of Marek's disease virus

Author:
Niikura, M.; Liu, H.C.; Dodgson, J.B.; Cheng, H.H.
Source:
Poultry science 2004 v.83 no.7 pp. 1117-1123
ISSN:
0032-5791
Subject:
chickens; screening; quantitative trait loci; hybrids; genetic resistance; host-pathogen relationships; protein-protein interactions; DNA primers; animal proteins; major histocompatibility complex; chromosome mapping; Mardivirus; viral proteins; Marek disease; virulence; single nucleotide polymorphism
Abstract:
... Genetic resistance to Marek's disease (MD) has been proposed as a method to augment current vaccinal control of MD. Although it is possible to identify QTL and candidate genes that are associated with MD resistance, it is necessary to integrate functional screens with linkage analysis to confirm the identity of true MD resistance genes. To help achieve this objective, a comprehensive 2-hybrid scre ...
Handle:
10113/9777
DOI:
10.1093/ps/83.7.1117
PubMed:
15285502

17. A computational and experimental approach to validating annotations and gene predictions in the Drosophila melanogaster genome

Author:
Yandell, M.; Bailey, A.M.; Misra, S.; Shu, S.; Wiel, C.; Evans-Holm, M.; Celniker, S.E.; Rubin, G.M.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2005 v.102 no.5 pp. 1566-1571
ISSN:
0027-8424
Subject:
insect proteins; DNA primers; bioinformatics; reverse transcriptase polymerase chain reaction; genes; genomics; Drosophila melanogaster; prediction
Abstract:
... Five years after the completion of the sequence of the Drosophila melanogaster genome, the number of protein-coding genes it contains remains a matter of debate; the number of computational gene predictions greatly exceeds the number of validated gene annotations. We have assembled a collection of >10,000 gene predictions that do not overlap existing gene annotations and have developed a process f ...
DOI:
10.1073/pnas.0409421102
PubMed:
15668397
PubMed Central:
PMC545494

18. A deletion in the STA1 promoter determines maltotriose and starch utilization in STA1+ Saccharomyces cerevisiae strains

Author:
Kristoffer Krogerus; Frederico Magalhães; Joosu Kuivanen; Brian Gibson
Source:
Applied microbiology and biotechnology 2019 v.103 no.18 pp. 7597-7615
ISSN:
0175-7598
Subject:
DNA primers; Saccharomyces cerevisiae; beers; dextrins; fermentation; gene editing; genes; glucan 1,4-alpha-glucosidase; hydrolysis; maltotriose; nucleotide sequences; polymerase chain reaction; starch; wort (brewing)
Abstract:
... Diastatic strains of Saccharomyces cerevisiae are common contaminants in beer fermentations and are capable of producing an extracellular STA1-encoded glucoamylase. Recent studies have revealed variable diastatic ability in strains tested positive for STA1, and here, we elucidate genetic determinants behind this variation. We show that poorly diastatic strains have a 1162-bp deletion in the promot ...
DOI:
10.1007/s00253-019-10021-y
PubMed:
31346683
PubMed Central:
PMC6719335

19. A duplex RT-PCR assay for detection of H9 subtype avian influenza viruses and infectious bronchitis viruses

Author:
Yan-di WEI; Wei-hua GAO; Hong-lei SUN; Chen-fang YU; Xing-yao PEI; Yi-peng SUN; Jin-hua LIU; Juan PU
Source:
Journal of integrative agriculture 2016 v.15 no.9 pp. 2105-2113
ISSN:
2095-3119
Subject:
DNA primers; Infectious bronchitis virus; Influenza A virus; chickens; egg production; eggs; financial economics; genes; hemagglutinins; infectious bronchitis; mixed infection; monitoring; nucleocapsid; pathogens; respiratory tract diseases; reverse transcriptase polymerase chain reaction; viruses; China
Abstract:
... H9 subtype avian influenza virus (AIV) and infectious bronchitis virus (IBV) are major pathogens circulating in poultry and have resulted in great economic losses due to respiratory disease and reduced egg production. As similar symptoms are elicited by the two pathogens, it is difficult for their differential diagnosis. So far, no reverse transcription-polymerase chain reaction (RT-PCR) assay has ...
DOI:
10.1016/S2095-3119(15)61316-8
PubMed:
32288952
PubMed Central:
PMC7128909

20. A duplex real-time polymerase chain reaction assay differentiates between Bolbphorus damnificus and Bolbophorus type II species cercariae

Author:
Matt J. Griffin; David J. Wise; Marlena C. Yost; Cynthia M. Doffitt; Linda M. Pote; Terrence E. Greenway; Lester H. Khoo
Source:
Journal of veterinary diagnostic investigation 2010 v.22 no. pp. 615-622
ISSN:
1943-4936
Subject:
cercariae; disease diagnosis; parasites; detection limit; Gastropoda; fish industry; quantitative analysis; DNA primers; ribosomal DNA; polymerase chain reaction; pathogen identification; snails; fish diseases; genes; Ictalurus punctatus; catfish; molecular systematics; trematode infections; Digenea (Trematoda)
Abstract:
... A duplex quantitative real-time polymerase chain reaction (qPCR) assay was developed to differentiate between Bolbophorus damnificus and Bolbophorus type II species cercariae. Both trematode species are prevalent throughout the commercial catfish industry,.as both infect the ram’s horn snail, Planobella trivolvis. Identification of the cercaria to species is important in catfish disease challenge ...
Handle:
10113/55483
DOI:
10.1177/104063871002200420
PubMed:
20622236