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You searched for: Subject term Danio rerio Remove constraint Subject term: Danio rerio Subject term liquid chromatography Remove constraint Subject term: liquid chromatography
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1. Comprehensive assessment of chemical residues in surface and wastewater using passive sampling, chemical, biological, and fish behavioral assays

Author:
Laura D. Brunelle; Irvin J. Huang; Luisa F. Angeles; Logan S. Running; Howard I. Sirotkin; Anne E. McElroy; Diana S. Aga
Source:
Science of the total environment 2022 v.828 pp. 154176
ISSN:
0048-9697
Subject:
Danio rerio; biomarkers; ciprofloxacin; environment; liquid chromatography; males; municipal wastewater; peptides; surface water; tandem mass spectrometry; vitellogenin; wastewater treatment; water quality; Hudson River
Abstract:
... Effluents from ten full-scale municipal wastewater treatment plants (WWTPs) that discharge into the Hudson River, surface waters, and wild-caught fish samples were analyzed using liquid chromatography with tandem mass spectrometry (LC/MS/MS) to examine the influence of wastewater discharge on the concentrations of contaminants of emerging concern (CECs) and their ecological impacts on fish. Analys ...
DOI:
10.1016/j.scitotenv.2022.154176

2. Catalytic ozonation of iohexol with α-Fe0.9Mn0.1OOH in water: Efficiency, degradation mechanism and toxicity evaluation

Author:
Pengwei Yan; Zhonglin Chen; Shuyu Wang; Yanchi Zhou; Li Li; Lei Yuan; Jimin Shen; Qianqian Jin; Xiaoxiao Zhang; Jing Kang
Source:
Journal of hazardous materials 2021 v.402 pp. 123574
ISSN:
0304-3894
Subject:
Danio rerio; X-radiation; catalysts; catalytic activity; dissolved organic carbon; heart rate; hydrolysis; liquid chromatography; mineralization; oxidation; ozonation; ozone; toxicity; water treatment
Abstract:
... Iohexol, a widely used iodinated X-ray contrast media, is difficult to completely degrade with the traditional water treatment process. Catalytic ozonation with synthesized α-Fe₀.₉Mn₀.₁OOH as the catalyst can significantly promote the degradation of iohexol relative to that with ozonation alone. Hydroxyl radicals play a predominant role during the degradation of iohexol. The effect of various fact ...
DOI:
10.1016/j.jhazmat.2020.123574

3. Detection of anti-cancer drugs and metabolites in the effluents from a large Brazilian cancer hospital and an evaluation of ecotoxicology

Author:
Mariana de Oliveira Klein; Sergio V. Serrano; Álvaro Santos-Neto; Claudinei da Cruz; Isabella Alves Brunetti; Daniel Lebre; Maíse Pastore Gimenez; Rui M. Reis; Henrique C.S. Silveira
Source:
Environmental pollution 2021 v.268 pp. 115857
ISSN:
0269-7491
Subject:
Danio rerio; cyclophosphamide; drug therapy; ecotoxicology; edema; fluorouracil; histology; hospitals; laws and regulations; liquid chromatography; liver; mass spectrometry; metabolites; municipal wastewater; necrosis; pollution; toxicity; wastewater treatment; Brazil; Europe
Abstract:
... The use of chemotherapy agents has been growing worldwide, due to the increase number of cancer cases. In several countries, mainly in Europe countries, these drugs have been detected in hospitals and municipal wastewaters. In Brazil this issue is poorly explored. The main goal of this study was to assess the presence of three anti-cancer drugs, 5-fluorouracil (5-FU), gemcitabine (GEM) and cycloph ...
DOI:
10.1016/j.envpol.2020.115857

4. Differential influences of (±) anatoxin-a on photolocomotor behavior and gene transcription in larval zebrafish and fathead minnows

Author:
Lea M. Lovin; Sujin Kim; Raegyn B. Taylor; Kendall R. Scarlett; Laura M. Langan; C. Kevin Chambliss; Saurabh Chatterjee; J. Thad Scott; Bryan W. Brooks
Source:
Environmental sciences Europe 2021 v.33 no.1 pp. 40
ISSN:
2190-4707
Subject:
Danio rerio; Pimephales promelas; caffeine; enantioselectivity; fish behavior; fish larvae; hepatotoxicity; liquid chromatography; neurotoxicity; neurotoxins; oxidative stress; pollution; tandem mass spectrometry; toxicology; transcription (genetics); water quality; Europe
Abstract:
... BACKGROUND: Though anatoxin-a (antx-a) is a globally important cyanobacterial neurotoxin in inland waters, information on sublethal toxicological responses of aquatic organisms is limited. We examined influences of (±) antx-a (11–3490 µg/L) on photolocomotor behavioral responses and gene transcription associated with neurotoxicity, oxidative stress and hepatotoxicity, in two of the most common alt ...
DOI:
10.1186/s12302-021-00479-x

5. Emerging contaminant occurrence and toxic effects on zebrafish embryos to assess the adverse effects caused by mixtures of substances in the environment

Author:
Gisela de Assis Martini; Cassiana Carolina Montagner; William Viveiros; Gilson Alves Quinaglia; Daniela Dayrell França; Nívea Cristina Guedes Munin; Mônica Lopes-Ferreira; Sizue Ota Rogero; José Roberto Rogero
Source:
Environmental science and pollution research 2021 v.28 no.16 pp. 20313-20329
ISSN:
0944-1344
Subject:
Danio rerio; anthropogenic activities; aquatic environment; bioassays; bisphenol A; caffeine; carbendazim; chemical analysis; fish; liquid chromatography; mass spectrometry; mortality; nontarget organisms; pollution; research; rivers; solid phase extraction; surface water; toxicity; water quality; Brazil
Abstract:
... The contaminants of emerging concern (CECs) have been receiving global attention due to their worldwide presence in water bodies. The CECs could be originated from synthetic or natural sources, and they are not commonly monitored, although these substances are continuously reaching the aquatic environment. The main goal of this study was to determine the occurrence of some target CECs in São Paulo ...
DOI:
10.1007/s11356-020-11963-x

6. Evaluation of 3,4,4,9-trichlorocarbanilide to zebrafish developmental toxicity based on transcriptomics analysis

Author:
Liting He; Yuanyao Chen; Zhiyong Hu; Yuan Zhang; Yongfeng Wang; Jiajing Wei; Zunpan Fan; Jia Xu; Meilin Peng; Kai Zhao; Huiping Zhang; Chunyan Liu
Source:
Chemosphere 2021 v.278 pp. 130349
ISSN:
0045-6535
Subject:
Danio rerio; aryl hydrocarbon receptors; edema; embryogenesis; gene expression regulation; heart; hemorrhage; liquid chromatography; organic matter; pericardial effusion; pollution; risk; tandem mass spectrometry; teratogenicity; toxicology; transcriptome; transcriptomics
Abstract:
... Triclocarban (TCC), considered an endocrine-disrupting, persistent, and bioaccumulating organic matter, has attracted a great deal of attention for its pollution and health risks. However, studies on its toxicological mechanism, especially for embryo development are limited. This article explores the cardiac developmental toxicity induced in zebrafish embryos after exposure to different TCC concen ...
DOI:
10.1016/j.chemosphere.2021.130349

7. Fucoxanthin-rich fraction from Sargassum fusiformis alleviates particulate matter-induced inflammation in vitro and in vivo

Author:
Yu-Lin Dai; Yun-Fei Jiang; Yu-An Lu; Jiang-Bo Yu; Min-Cheol Kang; You-Jin Jeon
Source:
Toxicology reports 2021 v.8 pp. 349-358
ISSN:
2214-7500
Subject:
Danio rerio; Sargassum; air pollution; cell death; culture media; dust; inducible nitric oxide synthase; inflammation; interleukin-6; keratinocytes; liquid chromatography; macroalgae; macrophages; mass spectrometry; models; necrosis; neoplasms; nitric oxide; particulates; prostaglandin synthase; prostaglandins; reactive oxygen species; toxicology
Abstract:
... Particulate matter (PM) contributes to air pollution and primarily originates from unregulated industrial emissions and seasonal natural dust emissions. Fucoxanthin (Fx) is a marine natural pigment from brown macroalgae that has been shown to have various beneficial effects on health. However, the effects of Fx on PM-induced toxicities in cells and animals have not been assessed. In this study, we ...
DOI:
10.1016/j.toxrep.2021.02.005

8. Is zebrafish (Danio rerio) water tank model applicable for the assessment of glucocorticoids metabolism? The budesonide assessment

Author:
Amanda Lessa Dutra de Araujo; Isabelle Karine da Costa Nunes; Vinicius Figueiredo Sardela; Henrique Marcelo Gualberto Pereira; Lucio Mendes Cabral; Carina de Souza Anselmo
Source:
Journal of chromatography 2021 v.1179 pp. 122826
ISSN:
1570-0232
Subject:
Danio rerio; biochemical pathways; biotransformation; cholinesterase; glucocorticoids; humans; liquid chromatography; mass spectrometry; metabolism; metabolites; models; prednisolone; sports; urine
Abstract:
... Knowledge of the metabolic profile is essential for doping control analysis in sport since most drugs are excreted after an elaborate biotransformation process. Currently, Zebrafish Water Tank (ZWT) model has been applied to investigate the metabolism of different doping agents. Nevertheless, the class of glucocorticoids has not been subjected to this model for metabolism studies. In the present w ...
DOI:
10.1016/j.jchromb.2021.122826

9. Mass spectrometry imaging in zebrafish larvae for assessing drug safety and metabolism

Author:
Mariana Asslan; Nidia Lauzon; Maja Beus; Dusica Maysinger; Simon Rousseau
Source:
Analytical and bioanalytical chemistry 2021 v.413 no.20 pp. 5135-5146
ISSN:
1618-2642
Subject:
Danio rerio; analytical chemistry; antineoplastic agents; desorption; digestive system; fecundity; histone deacetylase; intravenous injection; liquid chromatography; metabolism; phenotype; safety assessment; xenobiotics
Abstract:
... Drug safety assessment in the early phases of drug discovery is critical to facilitate the rapid development of novel therapeutics. Recently, teleost zebrafish (Danio rerio) has emerged as a promising vertebrate model for the assessment of drug safety. Zebrafish is a convenient model because of its small size, high fecundity, embryo transparency, and ex utero development. In this study, we develop ...
DOI:
10.1007/s00216-021-03476-4

10. Thyroid disrupting effects of low-dose dibenzothiophene and cadmium in single or concurrent exposure: New evidence from a translational zebrafish model

Author:
E. Guzzolino; M.S. Milella; F. Forini; M. Borsò; G. Rutigliano; F. Gorini; R. Zucchi; A. Saba; F. Bianchi; G. Iervasi; L. Pitto
Source:
Science of the total environment 2021 v.769 pp. 144703
ISSN:
0048-9697
Subject:
Danio rerio; cadmium; embryogenesis; energy; environment; fluorescence; genetically modified organisms; geometry; heart; homeostasis; human health; liquid chromatography; mass spectrometry; metabolism; models; pollutants; toxicity
Abstract:
... Thyroid hormones (THs) are major regulators of biological processes essential for correct development and energy homeostasis. Although thyroid disruptors can deeply affect human health, the impact of exogenous chemicals and in particular mixture of chemicals on different aspects of thyroid development and metabolism is not yet fully understood. In this study we have used the highly versatile zebra ...
DOI:
10.1016/j.scitotenv.2020.144703

11. Tracing anti‐osteoporosis components from raw and salt‐processed semen of Cuscuta chinensis by employing a biochemometrics strategy that integrates ultrasonic‐assisted extraction, quantitation, efficacy assessment in zebrafish, and grey relationship analysis

Author:
Yi Tao; Lin Chen; Meiling Pan; Fei Zhu; Jizhong Yan
Source:
Journal of separation science 2021 v.44 no.17 pp. 3229-3236
ISSN:
1615-9306
Subject:
Cuscuta; Danio rerio; chloramphenicol; liquid chromatography; p-coumaric acid; principal component analysis; semen; separation; tandem mass spectrometry
Abstract:
... Semen of Cuscuta chinensis has been reported to have an anti‐osteoporosis effect, however, the components which account for the anti‐osteoporosis effect have not been clarified. In this work we propose a biochemometrics strategy that integrates quantitation, anti‐osteoporosis evaluation in zebrafish, and grey relationship analysis for the identification of anti‐osteoporosis components from the sem ...
DOI:
10.1002/jssc.202100272

12. Using zebrafish embryo bioassays combined with high-resolution mass spectrometry screening to assess ecotoxicological water bodies quality status: A case study in Panama rivers

Author:
Estibali Wilkie Wilson; Verónica Castro; Raquel Chaves; Miguel Espinosa; Rosario Rodil; José Benito Quintana; Maria Natividade Vieira; Miguel M. Santos
Source:
Chemosphere 2021 v.272 pp. 129823
ISSN:
0045-6535
Subject:
Danio rerio; anthropogenic activities; bioassays; case studies; drugs; ecotoxicology; embryonic mortality; industrial wastewater; liquid chromatography; mass spectrometry; metabolites; toxicity; Panama
Abstract:
... Several studies show that many water bodies in developing countries are increasingly affected by anthropogenic pressure, such as agricultural activities, domestic and industrial wastewater. However, data is scarce in several of such countries, including Panama. Thus, in this work, the ecotoxicological status of selected rivers in Panama with distinct input sources were evaluated using the zebrafis ...
DOI:
10.1016/j.chemosphere.2021.129823

13. Antimicrobial activity of bacteriocin‐producing Carnobacterium spp. isolated from healthy Patagonian trout and their potential for use in aquaculture

Author:
Marisa E. Garcés; Nelda L. Olivera; Melania Fernández; Carla Riva Rossi; Cynthia Sequeiros
Source:
Aquaculture research 2020 v.51 no.11 pp. 4602-4612
ISSN:
1355-557X
Subject:
Carnobacterium divergens; Carnobacterium piscicola; Danio rerio; agar; anti-infective agents; antimicrobial properties; aquaculture; bacteria; bacteriocins; fish health; health effects assessments; intestines; liquid chromatography; nucleotide sequences; pathogens; peptides; ribosomal RNA; tandem mass spectrometry; thermal stability; trout
Abstract:
... In this study, bacteria from the intestines of Patagonian trout displaying fish pathogen antimicrobial activity were isolated and characterized. Isolates T4, T15 and M5 showed extracellular antimicrobial activity against the fish pathogen Carnobacterium maltaromaticum CECT 4020, by the agar well diffusion assay. They were identified as Gram‐positive catalase‐negative bacilli belonging to the genus ...
DOI:
10.1111/are.14806

14. Characterization of monoaminergic neurochemicals in the different brain regions of adult zebrafish

Author:
Marta Mayol-Cabré; Eva Prats; Demetrio Raldúa; Cristian Gómez-Canela
Source:
Science of the total environment 2020 v.745 pp. 141205
ISSN:
0048-9697
Subject:
Danio rerio; adults; brain; environment; liquid chromatography; models; neurotransmitters; tandem mass spectrometry
Abstract:
... Monoaminergic neurotransmitters are the main components that regulate of a lot of processes in the vertebrate brain. There is growing interest to monitor the changes produced in these neurochemicals due to the large number of exogenous agents, such as pharmaceuticals and drugs of abuse, targeting and affecting this system. Adult zebrafish (Danio rerio) shares the common neurotransmitter pathways a ...
DOI:
10.1016/j.scitotenv.2020.141205

15. Dammarane-Type Triterpenoids from the Roots of Rhus chinensis and Their Preventive Effects on Zebrafish Heart Failure and Thrombosis

Author:
Miao Ye; Wen Xu; Da-Qing He; Xian Wu; Wen-Fang Lai; Xiao-Qin Zhang; Yu Lin; Wei Xu; Xu-Wen Li
Source:
Journal of natural products 2020 v.83 no.2 pp. 362-373
ISSN:
1520-6025
Subject:
Danio rerio; Oriental traditional medicine; Rhus; bioassays; blood flow; cardiac output; coronary vessels; drugs; heart; heart failure; heart rate; liquid chromatography; mass spectrometry; moieties; quantum mechanics; roots; spectral analysis; therapeutics; thrombosis; triterpenoids
Abstract:
... Eight new dammarane-type triterpenoids (1–8), together with a related known analogue (9), were isolated from the roots of Rhus chinensis, a traditional Chinese medicine for treating coronary artery heart disease, guided by LC-MS analysis. Their structures were elucidated based on extensive spectroscopic analysis and quantum chemical calculations. Notably, compounds 1–7 and 9 possess an unusual 17α ...
DOI:
10.1021/acs.jnatprod.9b00857

16. Discovery and identification of proangiogenic chemical markers from Gastrodiae Rhizoma based on zebrafish model and metabolomics approach

Author:
Mengyujie Liu; Liang Zhao; Liwen Han; Haonan Li; Yongping Shi; Jing Cui; Chenyang Wang; Li Xu; Lihong Zhong
Source:
Phytochemical analysis 2020 v.31 no.6 pp. 835-845
ISSN:
0958-0344
Subject:
Danio rerio; Gastrodia elata; angiogenesis; atherosclerosis; bioactive properties; blood; citric acid; liquid chromatography; mass spectrometry; metabolomics; models; rhizomes; stigmasterol
Abstract:
... INTRODUCTION: Angiogenesis is closely related to a variety of diseases, and therapies based on angiogenesis are intensely investigated. Studies have shown that the use of Gastrodiae Rhizoma (GR, Gastrodia elata) can benefit the treatment of ischemic cardiovascular diseases and atherosclerosis by stimulating angiogenesis. OBJECTIVE: This study tested the angiogenesis effects of a group of chemical ...
DOI:
10.1002/pca.2949

17. Evolving high-throughput approaches to assess the environmental risk of sewage water at molecular level: comprehensive study

Author:
R. T. V. Vimala; J. Lija Escaline; Kadarkarai Murugan; S. Sivaramakrishnan
Source:
Environmental monitoring and assessment 2020 v.192 no.6 pp. 377
ISSN:
0167-6369
Subject:
Danio rerio; X-ray diffraction; aquatic organisms; dissolved organic matter; environmental impact; fluorescence; gas chromatography-mass spectrometry; liquid chromatography; models; municipal wastewater; risk; sewage; toxicity testing; volatile organic compounds
Abstract:
... Dissolved organic matter (DOM) especially anthropogenic compounds in sewage systems affects their ultimate fate in the environment which is challenging to ascertain the heterogenic nature of the compound and causes co-occurring effects in most aquatic samples. So, our study have focused on current approaches to the chemical and structural characterization of DOM with the detailed classification of ...
DOI:
10.1007/s10661-020-08258-6

18. Reproductive dysfunction linked to alteration of endocrine activities in zebrafish exposed to mono-(2-ethylhexyl) phthalate (MEHP)

Author:
Chang-Beom Park; Go-Eun Kim; Young Jun Kim; Jiwon On; Chang-Gyun Park; Young-Sang Kwon; Heesoo Pyo; Dong-Huk Yeom; Sung-Hee Cho
Source:
Environmental pollution 2020 v.265 pp. 114362
ISSN:
0269-7491
Subject:
Danio rerio; adults; aquatic environment; biosynthesis; chronic toxicity; cortisol; eggs; embryo (animal); endocrine-disrupting chemicals; estradiol; exposure duration; females; fish; liquid chromatography; males; messenger RNA; metabolites; phthalates; progesterone; reproductive toxicology; steroids; stress response; tandem mass spectrometry; toxicity testing; vitellogenin
Abstract:
... This study aimed to investigate the effect of mono-(2-ethylhexyl) phthalate (MEHP), one of the major phthalate metabolites that are widespread in aquatic environments, on reproductive dysfunction, particularly on endocrine activity in adult male and female zebrafish. For 21 days, the zebrafish were exposed to test concentrations of MEHP (0, 2, 10, and 50 μg/mL) that were determined based on the ef ...
DOI:
10.1016/j.envpol.2020.114362

19. Stereoselective uptake and metabolism of prothioconazole caused oxidative stress in zebrafish (Danio rerio)

Author:
Zhaoxian Zhang; Jing Zhang; Xuejun Zhao; Beibei Gao; Zongzhe He; Lianshan Li; Haiyan Shi; Minghua Wang
Source:
Journal of hazardous materials 2020 v.396 pp. 122756
ISSN:
0304-3894
Subject:
Danio rerio; adverse effects; antioxidant enzymes; biochemical pathways; disease control; enantioselectivity; enzyme activity; fungicides; glucuronic acid; grain crops; lethal concentration 50; liquid chromatography; mass spectrometry; metabolism; metabolites; nontarget organisms; oxidative stress; sulfates; toxicity; triazoles
Abstract:
... Prothioconazole (PTA) is a novel, broad-spectrum, chiral triazole fungicide that is mainly used to prevent and control the disease of cereal crops. However, the adverse effects of PTA and its major metabolite on nontarget organisms have aroused wide concern. In the present work, the acute toxic of the metabolite prothioconazole-desthio (PTA-desthio), with an LC₅₀ of 1.31 mg L⁻¹, was 3.5-fold more ...
DOI:
10.1016/j.jhazmat.2020.122756

20. Substrate multispecificity among 20β-hydroxysteroid dehydrogenase type 2 members

Author:
Janina Tokarz; Jutta Lintelmann; Gabriele Möller; Jerzy Adamski
Source:
Molecular and cellular endocrinology 2020 v.510 pp. 110822
ISSN:
0303-7207
Subject:
3alpha(or 20beta)-hydroxysteroid dehydrogenase; Danio rerio; biosynthesis; cortisol; cortisone; liquid chromatography; mass spectrometry; steroids
Abstract:
... Steroids regulate many physiological processes. Hydroxysteroid dehydrogenases (HSDs) modulate the levels of steroids in pre- and post-receptor metabolism. The subfamily of 20β-HSD type 2 currently comprises six members from six different species. The zebrafish ortholog converts cortisone to 20β-dihydrocortisone and is involved in the catabolism of the stress hormone cortisol. Here, we elucidated t ...
DOI:
10.1016/j.mce.2020.110822

21. Comprehensive chemical profiling of monascus-fermented rice product and screening of lipid-lowering compounds other than monacolins

Author:
Jin-Xiu Liang; Qun-Qun Zhang; Yan-Fei Huang; Han-Qing Pang; Xin-Guang Liu; Wen Gao; Ping Li; Hua Yang
Source:
Journal of ethnopharmacology 2019 v.238 pp. 111879
ISSN:
0378-8741
Subject:
Danio rerio; acids; amino acids; body weight changes; ergosterol; gas chromatography-mass spectrometry; glycerol; heart; hyperlipidemia; isoflavones; larvae; liquid chromatography; liver; low density lipoprotein cholesterol; mice; monacolins; nucleosides; phytosterols; red yeast rice; reference standards; screening; spectral analysis; therapeutics; traditional medicine; triacylglycerols; China
Abstract:
... Monascus-fermented rice product (MFRP) has been regarded as a dietary supplement and traditional medicine with circulation-promoting effects in China and other countries for centuries.This study was carried out to profile the chemical components in MFRP, and provide available information for elucidating the potential lipid-lowering compounds other than monacolins.High-performance liquid chromatogr ...
DOI:
10.1016/j.jep.2019.111879

22. Elemental imaging (LA-ICP-MS) of zebrafish embryos to study the toxicokinetics of the acetylcholinesterase inhibitor naled

Author:
Katharina Halbach; Stephan Wagner; Stefan Scholz; Till Luckenbach; Thorsten Reemtsma
Source:
Analytical and bioanalytical chemistry 2019 v.411 no.3 pp. 617-627
ISSN:
1618-2642
Subject:
Danio rerio; acetylcholinesterase; atomic absorption spectrometry; bromine; carbon; dichlorvos; ecotoxicology; embryo (animal); enzyme inhibition; enzyme inhibitors; image analysis; liquid chromatography; models; naled; pharmacokinetics; stable isotopes; tandem mass spectrometry
Abstract:
... The zebrafish embryo is an important model in ecotoxicology but the spatial distribution of chemicals and the relation to observed effects is not well understood. Quantitative imaging can help to gain insights into the distribution of chemicals in the zebrafish embryo. Laser ablation inductively coupled plasma-mass spectrometry (LA-ICP-MS) is used to quantify the uptake and the uptake kinetics of ...
DOI:
10.1007/s00216-018-1471-2
PubMed:
30443774
PubMed Central:
PMC6338705

23. Geographic-based metabolomic variation and toxicity analysis of Sutherlandia frutescens L. R.Br. – An emerging medicinal crop in South Africa

Author:
Samkele Zonyane; Longsheng Chen; Min-Jie Xu; Zhu-Nan Gong; Shuwen Xu; Nokwanda P. Makunga
Source:
Industrial crops and products 2019 v.133 pp. 414-423
ISSN:
0926-6690
Subject:
Danio rerio; Lessertia frutescens; acetonitrile; bioassays; chemical constituents of plants; cytotoxicity; domestication; embryo (animal); ethanol; formic acid; hatching; industry; larvae; leaves; lethal concentration 50; liquid chromatography; medicinal plants; metabolomics; mortality; processing technology; tandem mass spectrometry; therapeutics; South Africa
Abstract:
... Sutherlandia frutescens (syn. Lessertia frutescens), an indigenous southern African species, is important for the local and global phytomedicine's industry. As farming of medicinal plants in South Africa is not extensive, wild populations are also used as a source of material to bulk available cultivated yields prior to industrial processing. This may cause inconsistency in the quality of medicina ...
DOI:
10.1016/j.indcrop.2019.03.010

24. Hazard assessment of Maerua subcordata (Gilg) DeWolf. for selected endpoints using a battery of in vitro tests

Author:
Mebrahtom Gebrelibanos Hiben; Lenny Kamelia; Laura de Haan; Bert Spenkelink; Sebastiaan Wesseling; Jacques Vervoort; Ivonne M.C.M. Rietjens
Source:
Journal of ethnopharmacology 2019 v.241 pp. 111978
ISSN:
0378-8741
Subject:
Ames test; Danio rerio; Maerua; acute toxicity; agonists; aromatic compounds; biogenic amines; cardiomyocytes; chronic diseases; computer software; embryo (animal); embryonic stem cells; embryotoxicity; estrogen receptors; famine; food plants; fruit extracts; fruits; genotoxicity; hatching; hazard characterization; in vitro studies; isothiocyanates; leaf extracts; leaves; liquid chromatography; mass spectrometry; metabolomics; mutagenicity; prediction; seed extracts; traditional medicine; tubers; wild plants; Eastern Africa
Abstract:
... Maerua subcordata (Gilg) DeWolf is a medicinal and wild food plant growing mainly in east Africa. Especially its root tuber is widely used in traditional medicine to treat several infectious and chronic diseases but also in some toxicity implications like use as abortifacient.the present study applied in silico and in vitro tests to identify possible hazards of M. subcordata (fruit, leaf, root, se ...
DOI:
10.1016/j.jep.2019.111978

25. Integrating Molecular Networking and ¹H NMR To Target the Isolation of Chrysogeamides from a Library of Marine-Derived Penicillium Fungi

Author:
Xue-Mei Hou; Yue-Ying Li; Yun-Wei Shi; Yao-Wei Fang; Rong Chao; Yu-Cheng Gu; Chang-Yun Wang; Chang-Lun Shao
Source:
Journal of organic chemistry 2019 v.84 no.3 pp. 1228-1237
ISSN:
1520-6904
Subject:
Danio rerio; Penicillium chrysogenum; angiogenesis; fungi; isomerization; isotope labeling; liquid chromatography; moieties; nuclear magnetic resonance spectroscopy; organic chemistry; organic compounds
Abstract:
... A challenging problem in natural product discovery is to rapidly dereplicate known compounds and expose novel ones from complicated components. Herein, integrating the LC-MS/MS-dependent molecular networking and ¹H NMR techniques efficiently and successfully enabled the targeted identification of seven new cyclohexadepsipeptides, chrysogeamides A–G (1–7), from the coral-derived fungus Penicillium ...
DOI:
10.1021/acs.joc.8b02614

26. Investigations on the wound healing properties of Onosma dichroantha Boiss root extracts

Author:
F. Safavi; M.M. Farimani; M. Golalipour; P.-C. Leung; K.-M. Lau; H.-F. Kwok; C.-W. Wong; H. Bayat; C.B.-S. Lau
Source:
South African journal of botany 2019 v.125 pp. 344-352
ISSN:
0254-6299
Subject:
Danio rerio; Onosma; bioactive compounds; cyclohexanes; embryo (animal); ethyl acetate; fibroblasts; genetically modified organisms; inflammation; liquid chromatography; mass spectrometry; methanol; root extracts; roots; shikonin; tissue repair; traditional medicine; Iran
Abstract:
... Onosma dichroantha Boiss. (Boraginaceae), has been used in traditional medicine in Iran as anti-inflammatory for healing wounds and burns. This study aimed to investigate the wound healing potential of different extracts of roots of Onosma dichroantha. The roots were extracted successively with cyclohexane (CE), ethyl acetate (EtOAc), and methanol (MeOH). The extracts were tested for anti-inflamma ...
DOI:
10.1016/j.sajb.2019.08.005

27. LC-MS/MS method for the determination of organophosphorus pesticides and their metabolites in salmon and zebrafish fed with plant-based feed ingredients

Author:
Borja Garlito; María Ibáñez; Tania Portolés; Roque Serrano; Heidi Amlund; Anne-Katrine Lundebye; Monica Sanden; Marc H. G. Berntssen; Félix Hernández
Source:
Analytical and bioanalytical chemistry 2019 v.411 no.27 pp. 7281-7291
ISSN:
1618-2642
Subject:
Danio rerio; Salmo salar; aquaculture feeds; bile; chlorpyrifos-methyl; fish culture; fish meal; fish oils; food production; ingredients; kidneys; liquid chromatography; liver; metabolites; muscles; pirimiphos-methyl; salmon; tandem mass spectrometry; water
Abstract:
... The composition of Atlantic salmon feed has changed considerably over the last two decades from being marine-based (fishmeal and fish oil) to mainly containing plant ingredients. Consequently, concern related to traditional persistent contaminants typically associated with fish-based feed has been replaced by other potential contaminants not previously associated with salmon farming. This is the c ...
DOI:
10.1007/s00216-019-02104-6

28. Metabolomics for Biomarker Discovery in Fermented Black Garlic and Potential Bioprotective Responses against Cardiovascular Diseases

Author:
Xuanming Zhang; Yongping Shi; Lizhen Wang; Xiaobin Li; Shanshan Zhang; Ximin Wang; Meng Jin; Chung-Der Hsiao; Houwen Lin; Liwen Han; Kechun Liu
Source:
Journal of agricultural and food chemistry 2019 v.67 no.44 pp. 12191-12198
ISSN:
1520-5118
Subject:
Amadori products; Danio rerio; angiogenesis; antibacterial properties; apoptosis; biomarkers; cardioprotective effect; endothelial cells; garlic; liquid chromatography; metabolomics; mitogen-activated protein kinase; phosphorylation; risk reduction; thrombosis
Abstract:
... Fermented black garlic has multiple beneficial biological activities, including cardiovascular protection, anticancer, hepatoprotective, and antibacterial properties. In this study, metabolic differences in the properties of black and fresh garlic were investigated via liquid chromatography quadrupole/time-of-flight-based metabolomics, leading to the identification of characteristic components. Fe ...
DOI:
10.1021/acs.jafc.9b04073

29. Structure Elucidation and Relative Toxicity of (24R)-24-Hydroxyyessotoxin from a Namibian Isolate of Gonyaulax spinifera

Author:
Isabelle Rajotte; Cheryl Rafuse; Elliott J. Wright; John C. Achenbach; Lee D. Ellis; Pearse McCarron
Source:
Journal of natural products 2019 v.82 no.7 pp. 1945-1952
ISSN:
1520-6025
Subject:
Danio rerio; Gonyaulax; biomass; hydroxylation; liquid chromatography; mass spectrometry; nuclear magnetic resonance spectroscopy; solvents; toxicity; yessotoxin
Abstract:
... Liquid chromatography–high-resolution mass spectrometry (LC-HRMS) analysis of a Namibian strain of Gonyaulax spinifera showed the presence of a number of yessotoxins (YTXs). Principal among these were YTX (1), homoYTX (2), and a tentative hydroxylated analogue that did not correspond to any previously confirmed YTX structures. Culturing the G. spinifera strain afforded sufficient biomass for purif ...
DOI:
10.1021/acs.jnatprod.9b00318

30. Assessment of endocrine disruptors effects on zebrafish (Danio rerio) embryos by untargeted LC-HRMS metabolomic analysis

Author:
Elena Ortiz-Villanueva; Joaquim Jaumot; Rubén Martínez; Laia Navarro-Martín; Benjamin Piña; Romà Tauler
Source:
Science of the total environment 2018 v.635 pp. 156-166
ISSN:
0048-9697
Subject:
Danio rerio; absorption; amino acids; aquatic organisms; bisphenol A; carboxylic acids; chemometrics; embryo (animal); embryogenesis; endocrine-disrupting chemicals; genetic markers; lipids; liquid chromatography; mass spectrometry; mechanism of action; metabolism; metabolites; metabolomics; nutrients; perfluorooctane sulfonic acid; peroxisome proliferator-activated receptors; phenotype; purines; signal transduction; toxicity; tributyltin
Abstract:
... Bisphenol A (BPA), perfluorooctane sulfonate (PFOS), and tributyltin (TBT) are emerging endocrine disruptors (EDCs) with still poorly defined mechanisms of toxicity and metabolic effects in aquatic organisms. We used an untargeted liquid chromatography-high resolution mass spectrometry (LC-HRMS) metabolomic approach to study the effects of sub-lethal doses of these three EDCs on the metabolic prof ...
DOI:
10.1016/j.scitotenv.2018.03.369

31. Comparative cardio and developmental toxicity induced by the popular medicinal extract of Sutherlandia frutescens (L.) R.Br. detected using a zebrafish Tuebingen embryo model

Author:
Longsheng Chen; Minjie Xu; Zhunan Gong; Samkele Zonyane; Shuwen Xu; Nokwanda P. Makunga
Source:
BMC complementary and alternative medicine 2018 v.18 no.1 pp. 273
ISSN:
1472-6882
Subject:
Danio rerio; Lessertia frutescens; alternative medicine; animals; bioassays; biochemical polymorphism; chemical composition; chemometrics; cytotoxicity; edema; embryo culture; ethanol; hemorrhage; larvae; liquid chromatography; maximum tolerated concentration; medicinal plants; metabolomics; models; neoplasms; pericardium; tandem mass spectrometry; teratogenicity; traditional medicine; yolk sac; South Africa
Abstract:
... BACKGROUND: Sutherlandia frutescens is one of the most promising commercialized, indigenous and medicinal plants of South Africa that is used as an immune-booster, and a traditional treatment for cancer. However, few studies report on its toxicology and dosage in vivo. There is still room to better understand its cytotoxicity effects in animal systems. METHODS: We prepared two extracts, one with 8 ...
DOI:
10.1186/s12906-018-2303-9
PubMed:
30290800
PubMed Central:
PMC6173916

32. Comprehensive characterization of neurochemicals in three zebrafish chemical models of human acute organophosphorus poisoning using liquid chromatography-tandem mass spectrometry

Author:
Cristian Gómez-Canela; Daniel Tornero-Cañadas; Eva Prats; Benjamí Piña; Romà Tauler; Demetrio Raldúa
Source:
Analytical and bioanalytical chemistry 2018 v.410 no.6 pp. 1735-1748
ISSN:
1618-2642
Subject:
Danio rerio; acetylcholine; animal models; central nervous system; developmental stages; dopamine; epinephrine; gamma-aminobutyric acid; glutamic acid; histamine; humans; ionization; liquid chromatography; metabolism; metabolites; monitoring; multiresidue analysis; neurophysiology; neurotoxins; neurotransmitters; norepinephrine; organophosphorus compounds; phospholipids; poisoning; serotonin; structure-activity relationships; tandem mass spectrometry
Abstract:
... There is a growing interest in biological models to investigate the effect of neurotransmitter dysregulation on the structure and function of the central nervous system (CNS) at different stages of development. Zebrafish, a vertebrate model increasingly used in neurobiology and neurotoxicology, shares the common neurotransmitter systems with mammals, including glutamate, GABA, glycine, dopamine, n ...
DOI:
10.1007/s00216-017-0827-3

33. Degradation characterization and pathway analysis of chlortetracycline and oxytetracycline in a microbial fuel cell

Author:
Ji Wang; Boyi Zhou; Ruijia Ge; Tian-shun Song; Jinping Yu; Jingjing Xie
Source:
RSC advances 2018 v.8 no.50 pp. 28613-28624
ISSN:
2046-2069
Subject:
Azospirillum; Burkholderia; Danio rerio; Stenotrophomonas; anodes; biofilm; chlortetracycline; high-throughput nucleotide sequencing; human health; liquid chromatography; mass spectrometry; microbial fuel cells; oxytetracycline; toxicity; toxicity testing; wastewater
Abstract:
... The wide presence of antibiotics in the environment has raised concerns about their potential impact on ecological and human health. This study was conducted to evaluate the degradation of antibiotics (chlortetracycline (CTC) and oxytetracycline (OTC)) in microbial fuel cells (MFCs) and the change of toxicity. The degradation rates of 60 mg L⁻¹ CTC and OTC in the MFCs were 74.2% and 78%, respectiv ...
DOI:
10.1039/c8ra04904a

34. Emerging contaminants in Brazilian rivers: Occurrence and effects on gene expression in zebrafish (Danio rerio) embryos

Author:
Juliana C.V. Sposito; Cassiana C. Montagner; Marta Casado; Laia Navarro-Martín; Julio César Jut Solórzano; Benjamin Piña; Alexeia B. Grisolia
Source:
Chemosphere 2018 v.209 pp. 696-704
ISSN:
0045-6535
Subject:
Danio rerio; atrazine; bisphenol A; caffeine; embryo (animal); estrogens; gene expression; human health; imidacloprid; liquid chromatography; mass spectrometry; pollution; rivers; solid phase extraction; surface water; tebuthiuron; Brazil
Abstract:
... Emerging contaminants (ECs) are synthetic or naturally occurring chemicals that are not commonly monitored despite having the potential of entering the environment and causing adverse ecological and/or human health effects. This study aimed to determine whether ECs are present in the surface waters of two rivers in Mato Grosso do Sul State, Brazil, and evaluate the effects of ECs mixtures at envir ...
DOI:
10.1016/j.chemosphere.2018.06.046

35. Identification, growth and toxicity assessment of Coolia Meunier (Dinophyceae) from Nova Scotia, Canada

Author:
Nancy I. Lewis; Jennifer L. Wolny; John Claude Achenbach; Lee Ellis; Joseph S. Pitula; Cheryl Rafuse; Detbra S. Rosales; Pearse McCarron
Source:
Harmful algae 2018 v.75 pp. 45-56
ISSN:
1568-9883
Subject:
Danio rerio; Dinophyceae; bioassays; cell growth; embryo (animal); embryotoxicity; fish; geographical distribution; liquid chromatography; mass spectrometry; methanol; microorganisms; mucilages; photons; poisonous algae; scanning electron microscopy; temperature; water quality; Mediterranean Sea; Nova Scotia; Rhode Island
Abstract:
... Benthic dinoflagellates of the toxigenic genus Coolia Meunier (Dinophyceae) are known to have a global distribution in both tropical and temperate waters. The type species, C. monotis, has been reported from the Mediterranean Sea, the NE Atlantic and from Rhode Island, USA in the NW Atlantic, whereas other species in the genus have been reported from tropical locations. Coolia cells were observed ...
DOI:
10.1016/j.hal.2018.04.001

36. Metabolism of five diterpenoid lactones from Dioscorea bulbifera tubers in zebrafish

Author:
Wei Shi; Jie Ling; Li-Long Jiang; Dong-Sheng Zhao; Ling-Li Wang; Zi-Tian Wu; Ping Li; Ying-Jie Wei; Hui-Jun Li
Source:
RSC advances 2018 v.8 no.14 pp. 7765-7773
ISSN:
2046-2069
Subject:
Danio rerio; Dioscorea bulbifera; adults; aqueous solutions; biochemical pathways; diterpenoids; glutathione; hepatotoxicity; lactones; liquid chromatography; mass spectrometry; medicinal plants; metabolism; metabolites; tubers
Abstract:
... Diterpenoid lactones (DLs) have been reported to be the main hepatotoxic constituents in Dioscorea bulbifera tubers (DBT), a traditional Chinese medicinal herb. The acquisition of early information regarding its metabolism is critical for evaluating the potential hepatotoxicity of DLs. We investigated, for the first time, the main metabolites of diosbulbin A (DIOA), diosbulbin C (DIOC), diosbulbin ...
DOI:
10.1039/c7ra12910f

37. Multiple vitellogenins in zebrafish (Danio rerio): quantitative inventory of genes, transcripts and proteins, and relation to egg quality

Author:
Ozlem Yilmaz; Amélie Patinote; Thaovi Nguyen; Julien Bobe
Source:
Fish physiology and biochemistry 2018 v.44 no.6 pp. 1509-1525
ISSN:
0920-1742
Subject:
Danio rerio; animal tissues; egg quality; eggs; females; genes; intestines; liquid chromatography; liver; messenger RNA; polypeptides; protein content; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; secretion; sequence analysis; tandem mass spectrometry; vitellogenin
Abstract:
... Scrutiny of the zebrafish (Danio rerio) genomic database confirmed eight functional vitellogenin (vtg) genes, each with one or two transcript variants, and the encoded Vtg polypeptides were structurally and functionally characterized in detail by in silico and experimental analyses. There were five type I (vtgs1, 4, 5, 6, and 7), two type II (vtg2 and vtg8), and one type III (vtg3) vtg gene(s) enc ...
DOI:
10.1007/s10695-018-0524-y

38. Validated LC-MS/MS method for simultaneous analysis of 21 cephalosporins in zebrafish for a drug toxicity study

Author:
Ying Liu; Jingpu Zhang; Changqin Hu
Source:
Analytical biochemistry 2018 v.558 pp. 28-34
ISSN:
0003-2697
Subject:
Danio rerio; accuracy; cephalosporins; chemical species; detection limit; drug toxicity; electrospray ionization mass spectrometry; formic acid; liquid chromatography; methanol; models; monitoring; precision; sampling; tandem mass spectrometry; toxicity testing
Abstract:
... Zebrafish model was used to perform this drug toxicity study. In this study, we monitored the absorption of drugs in zebrafish. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed in this experiment. The LC-MS/MS method was used for the simultaneous quantification of 21 cephalosporins in zebrafish. To prepare samples, rapid protein precipitation was performed with 1% f ...
DOI:
10.1016/j.ab.2018.08.001

39. A comparative study on chemical composition of total saponins extracted from fermented and white ginseng under the effect of macrophage phagocytotic function

Author:
Dan Xiao; Yang Xiu; Hao Yue; Xiuli Sun; Huanxi Zhao; Shuying Liu
Source:
Journal of Ginseng Research 2017 v.41 no.3 pp. 379-385
ISSN:
1226-8453
Subject:
Danio rerio; Paecilomyces; Panax; bioactive properties; comparative study; culture media; fermentation; ginsenosides; liquid chromatography; macrophages; mass spectrometry; metabolites; molecular weight; multivariate analysis; raw materials; research
Abstract:
... In this study, white ginseng was used as the raw material, which was fermented with Paecilomyces hepiali through solid culture medium, to produce ginsenosides with modified chemical composition. The characteristic chemical markers of the products thus produced were investigated using rapid resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (RRLC–QTOF–MS). Che ...
DOI:
10.1016/j.jgr.2017.03.009

40. Assessment of chlorpyrifos toxic effects in zebrafish (Danio rerio) metabolism

Author:
Cristian Gómez-Canela; Eva Prats; Benjamí Piña; Romà Tauler
Source:
Environmental pollution 2017 v.220 pp. 1231-1243
ISSN:
0269-7491
Subject:
metabolism; chlorpyrifos; oxidative stress; muscles; exposure duration; data collection; least squares; mass spectrometry; chemometrics; neurotransmitters; toxicity; Danio rerio; liquid chromatography; metabolites
Abstract:
... In this work the effect of chlorpyrifos exposure on metabolic profiles of zebrafish muscle was evaluated by liquid chromatography coupled to high resolution mass spectrometry. Different chemometric tools based on the selection of Regions of Interest and on Multivariate Curve-Resolution-Alternating Least Squares are proposed for the analysis of the complex data sets generated in the different expos ...
DOI:
10.1016/j.envpol.2016.11.010

41. Bisphenol A alternatives in thermal paper from the Netherlands, Spain, Sweden and Norway. Screening and potential toxicity

Author:
Maria K. Björnsdotter; Willem Jonker; Jessica Legradi; Jeroen Kool; Ana Ballesteros-Gómez
Source:
Science of the total environment 2017 v.601-602 pp. 210-221
ISSN:
0048-9697
Subject:
Danio rerio; additives; bioassays; bisphenol A; bisphenol S; cash receipts; color; dose response; estrogenic properties; liquid chromatography; locomotion; mass spectrometry; neurotoxicity; paper; screening; teratogenicity; Netherlands; Norway; Spain; Sweden
Abstract:
... Thermal paper contains potentially toxic additives, such as bisphenol A (BPA), as a common color developer. Because of its known endocrine disrupting effects, structural analogues to BPA, such as bisphenol S (BPS), D-8 and Pergafast 201, have been used as alternatives, but little is known about the presence and toxicological effects of alternatives other than BPS. In this study, thermal paper is s ...
DOI:
10.1016/j.scitotenv.2017.05.171

42. HR-LC-MS based analysis of two antibacterial metabolites from a marine sponge symbiont Streptomyces pharmamarensis ICN40

Author:
Francis-Joseph Rosemary Sharmila Joseph; Appadurai Muthamil Iniyan; Samuel Gnana Prakash Vincent
Source:
Microbial pathogenesis 2017 v.111 pp. 450-457
ISSN:
0882-4010
Subject:
Danio rerio; Porifera; Streptomyces; antibacterial properties; antibiotics; clinical trials; coasts; fermentation; lethal concentration 50; liquid chromatography; mass spectrometry; metabolites; methicillin-resistant Staphylococcus aureus; minimum inhibitory concentration; neoplasm cells; symbionts; thrombosis; India
Abstract:
... On the effort to screen antibiotics against Methicillin resistant Staphylococcus aureus (MRSA), an actinomycete strain which can produce bactericidal compound was isolated from a marine sponge of Kanyakumari Coast, India. Two anti-MRSA compounds (PVI401 and PVI402) were isolated from the fermentation plates of Streptomyces pharmamarensis ICN40. TLC bioautography analysis yielded two active spots w ...
DOI:
10.1016/j.micpath.2017.09.033

43. Metabolic disruption of zebrafish (Danio rerio) embryos by bisphenol A. An integrated metabolomic and transcriptomic approach

Author:
Elena Ortiz-Villanueva; Laia Navarro-Martín; Joaquim Jaumot; Fernando Benavente; Victoria Sanz-Nebot; Benjamín Piña; Romà Tauler
Source:
Environmental pollution 2017 v.231 pp. 22-36
ISSN:
0269-7491
Subject:
Danio rerio; adverse effects; biomarkers; bisphenol A; data collection; endocrine-disrupting chemicals; genes; high-throughput nucleotide sequencing; liquid chromatography; mass spectrometry; metabolism; metabolites; metabolome; metabolomics; multivariate analysis; phenotype; prediction; prostaglandins; signal transduction; toxic substances; transcriptomics
Abstract:
... Although bisphenol A (BPA) is commonly recognized as an endocrine disruptor, the metabolic consequences of its exposure are still poorly understood. In this study, we present a non-targeted LC-MS based metabolomic analysis in combination with a full-genome, high-throughput RNA sequencing (RNA-Seq) to reveal the metabolic effects and the subjacent regulatory pathways of exposing zebrafish embryos t ...
DOI:
10.1016/j.envpol.2017.07.095

44. Peptidomics of the zebrafish Danio rerio: In search for neuropeptides

Author:
Kristien A. Van Camp; Geert Baggerman; Ronny Blust; Steven J. Husson
Source:
Journal of proteomics 2017 v.150 pp. 290-296
ISSN:
1874-3919
Subject:
Danio rerio; G-protein coupled receptors; brain; ecotoxicology; enzymes; fish; genes; liquid chromatography; mass spectrometry; models; neuropeptides; neurophysiology; translation (genetics)
Abstract:
... (Neuro)peptides are small messenger molecules that are derived from larger, inactive precursor proteins by the highly controlled action of processing enzymes. These biologically active peptides can be found in all metazoan species where they orchestrate a wide variety of physiological processes. Obviously, detailed knowledge on the actual peptide sequences, including the potential existence of tru ...
DOI:
10.1016/j.jprot.2016.09.015

45. Proteomics analysis of zebrafish brain following chronically exposed to bisphenol A

Author:
Xiangyang Wu; Chongwei Lu; Xing Dong; Zhen Zhang; Ming Yang; Hai Xu
Source:
Toxicological and environmental chemistry 2017 v.99 no.3 pp. 469-481
ISSN:
1029-0486
Subject:
Danio rerio; aquatic environment; biosynthesis; bisphenol A; brain; calcium signaling; chemistry; chronic exposure; cytoplasm; endocrine-disrupting chemicals; fish; gene expression regulation; liquid chromatography; males; neurotoxicity; plasticizers; protein synthesis; proteins; proteomics; starch; sucrose; tandem mass spectrometry
Abstract:
... Bisphenol A, a plastic monomer and plasticizer, is a well-known endocrine disrupter, widely present in the aquatic environment, but little is known regarding its neurotoxicity in fish. Herein, we investigated its effects on male zebrafish brain. Zebrafish were exposed to 10 µg/L BPA for 45 days. An isobaric tags for relative and absolute quantitation approach coupled with nano high-performance liq ...
DOI:
10.1080/02772248.2016.1180694

46. Sub-lethal pharmaceutical hazard tracking in adult zebrafish using untargeted LC–MS environmental metabolomics

Author:
Ryan B. De Sotto; Carl D. Medriano; Yunchul Cho; Hyuk Kim; In-Young Chung; Kwang-Seol Seok; Kyung Guen Song; Seok Won Hong; Youngja Park; Sungpyo Kim
Source:
Journal of hazardous materials 2017 v.339 pp. 63-72
ISSN:
0304-3894
Subject:
Danio rerio; acute toxicity; adults; adverse effects; anthropogenic activities; aquatic environment; bioactive compounds; clarithromycin; fish; florfenicol; larvae; liquid chromatography; mass spectrometry; metabolites; metabolomics; nontarget organisms; swimming; toxicity testing
Abstract:
... Antibiotics in the aquatic environment are dispersed through anthropogenic activities at low concentrations. Despite their sub lethal concentration, these biologically active compounds may still have adverse effects to non-target species. This study examined the response of adult zebrafish to 0.1mg/L concentration of clarithromycin, florfenicol, sulfamethazine, and their mixture using environmenta ...
DOI:
10.1016/j.jhazmat.2017.06.009

47. Discovery, Synthesis, and Functional Characterization of a Novel Neuroprotective Natural Product from the Fruit of Alpinia oxyphylla for use in Parkinson’s Disease Through LC/MS-Based Multivariate Data Analysis-Guided Fractionation

Author:
Li Guohui; Zhang Zaijun; Quan Quan; Jiang Renwang; Szeto Samuel S.W.; Yuan Shuai; Wong Wing-tak; Lam Herman H. C.; Lee Simon Ming-Yuen; Chu Ivan K.
Source:
Journal of Proteome Research 2016 v.15 no.8 pp. 2595-2606
ISSN:
1535-3907
Subject:
Alpinia oxyphylla; Danio rerio; Parkinson disease; Western blotting; bioactive compounds; cerebellum; chemometrics; fractionation; ingredients; liquid chromatography; mass spectrometry; metabolomics; mice; neurons; neuroprotective effect; proteome; proteomics
Abstract:
... Herein we report the discovery of a novel lead compound, oxyphylla A [(R)-4-(2-hydroxy-5-methylphenyl)-5-methylhexanoic acid] (from the fruit of Alpinia oxyphylla), which functions as a neuroprotective agent against Parkinson’s disease. To identify a shortlist of candidates from the extract of A. oxyphylla, we employed an integrated strategy combining liquid chromatography/mass spectrometry, bioac ...
DOI:
10.1021/acs.jproteome.6b00152

48. LC-MS/MS determination of tralopyril in water samples

Author:
Isabel B. Oliveira; René Schönenberger; Carlos M. Barroso; Marc J.-F. Suter
Source:
Chemosphere 2016 v.145 pp. 445-449
ISSN:
0045-6535
Subject:
Danio rerio; antifouling agents; biocides; dimethyl sulfoxide; half life; liquid chromatography; rivers; seawater; tandem mass spectrometry
Abstract:
... A targeted analytical method was established to determine tralopyril (4-bromo-2-(4-chlorophenyl)-5-(trifluoromethyl)-1H-pyrrole-3-carbonitrile) in water. This compound has been recently introduced as a biocide in ship antifouling paints, becoming a potential new environmental contaminant. The method presented here allows for the first time the direct determination of tralopyril in environmental sa ...
DOI:
10.1016/j.chemosphere.2015.11.098

49. Metabolism of clofibric acid in zebrafish embryos (Danio rerio) as determined by liquid chromatography–high resolution–mass spectrometry

Author:
Stephan Brox; Bettina Seiwert; Nora Haase; Eberhard Küster; Thorsten Reemtsma
Source:
Comparative biochemistry and physiology 2016 v.185-186 pp. 20-28
ISSN:
1532-0456
Subject:
Danio rerio; biotransformation; carnitine; clofibric acid; ecotoxicology; enzymes; excretion; glucuronic acid; liquid chromatography; mass spectrometry; metabolism; screening; sulfates; taurine; toxicity testing; xenobiotics
Abstract:
... The zebrafish embryo (ZFE) is increasingly used in ecotoxicology research but detailed knowledge of its metabolic potential is still limited. This study focuses on the xenobiotic metabolism of ZFE at different life-stages using the pharmaceutical compound clofibric acid as study compound. Liquid chromatography with quadrupole-time-of-flight mass spectrometry (LC-QToF-MS) is used to detect and to i ...
DOI:
10.1016/j.cbpc.2016.02.007

50. Toxicity assessment of New Zealand and Pacific dinoflagellates Ostreopsis and Gambierdiscus (Dinophyceae) extracts using bioassays

Author:
PA Argyle; DT Harwood; LL Rhodes; O Champeau; LA Tremblay
Source:
New Zealand journal of marine and freshwater research 2016 v.50 no.3 pp. 444-456
ISSN:
1175-8805
Subject:
Danio rerio; Evechinus; Gambierdiscus; algae; bioassays; fauna; fish; flora; human diseases; larvae; larval development; liquid chromatography; maitotoxin; screening; tandem mass spectrometry; toxicity; New Zealand
Abstract:
... The dinoflagellate genus Ostreopsis produces palytoxin-like compounds (PLTXs) and the genus Gambierdiscus produces ciguatoxins (CTXs) and maitotoxin (MTX). Human illness has been recorded following the consumption of CTX-contaminated finfish. Their effects on the flora and fauna in the environment are largely unexplored. Toxin profiles of extracts of Ostreopsis and Gambierdiscus isolates from the ...
DOI:
10.1080/00288330.2016.1159581

51. Toxicokinetics of Polar Chemicals in Zebrafish Embryo (Danio rerio): Influence of Physicochemical Properties and of Biological Processes

Author:
Brox Stephan; Seiwert Bettina; Küster Eberhard; Reemtsma Thorsten
Source:
Environmental Science & Technology 2016 v.50 no.18 pp. 10264-10272
ISSN:
1520-5851
Subject:
Danio rerio; active ingredients; atropine; benzocaine; bioaccumulation factor; biotransformation; chloramphenicol; cimetidine; colchicine; developmental stages; liquid chromatography; mass spectrometry; membrane permeability; metribuzin; models; pharmacokinetics; phenytoin; physicochemical properties; polar compounds; sulfamethoxazole; thiacloprid; transporters; valproic acid
Abstract:
... The time-resolved uptake of 17 nonionic and ionic polar compounds (logD ≤ 2) with a diversity of functional groups into zebrafish embryos (ZFE) was studied over 96 h of exposure. Among them were pharmaceuticals, pesticides and plant active ingredients. Uptake rates for the diffusion controlled passive uptake through the ZFE membrane ranged from 0.02 to 24 h–¹ for the nonionic compounds and were sl ...
DOI:
10.1021/acs.est.6b04325

52. Analysis of 17 neurotransmitters, metabolites and precursors in zebrafish through the life cycle using ultrahigh performance liquid chromatography–tandem mass spectrometry

Author:
A. Santos-Fandila; E. Vázquez; A. Barranco; A. Zafra-Gómez; A. Navalón; R. Rueda; M. Ramírez
Source:
Journal of Chromatography B 2015 v.1001 pp. 191-201
ISSN:
1570-0232
Subject:
Danio rerio; acetylcholine; adulthood; agmatine; betaine; brain; choline; detection limit; dopamine; epinephrine; formic acid; gamma-aminobutyric acid; glutamic acid; glutamine; ionization; liquid chromatography; metabolites; methanol; neurophysiology; neurotransmitters; norepinephrine; serotonin; spectrometers; statistical analysis; tandem mass spectrometry; tryptophan; tyrosine
Abstract:
... An ultrahigh performance liquid chromatography–tandem mass spectrometry method for the identification and quantification of neurotransmitters, metabolites and precursors at different stages in zebrafish life was developed. Betaine, glutamine, glutamic acid, γ-aminobutyric acid, phosphocholine, glycerophosphocholine, cytidine 5′-diphosphocholine, choline, acetylcholine, dopamine, norepinephrine, se ...
DOI:
10.1016/j.jchromb.2015.07.040

53. Determination of 26 endocrine disrupting chemicals in fish and water using modified QuEChERS combined with solid-phase extraction and UHPLC-MS/MS

Author:
Mei Yang; Youning Ma; Wenjun Gui; Yiping Ren; Guonian Zhu; Yihua Liu
Source:
Analytical methods 2015 v.7 no.19 pp. 8418-8431
ISSN:
1759-9679
Subject:
Danio rerio; acetonitrile; adverse effects; androgens; chemical species; detection limit; endocrine-disrupting chemicals; estrogens; ethyl acetate; fish; fisheries; glucocorticoids; humans; isotope labeling; liquid chromatography; mineralocorticoids; monitoring; progesterone; risk assessment; screening; solid phase extraction; standard deviation; tandem mass spectrometry; tap water; thyroid hormones; toxicology; trenbolone; wildlife
Abstract:
... Endocrine-disrupting chemicals (EDCs) in the environment have adverse effects on humans and wildlife. A method based on ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for the determination of 26 EDCs (including five estrogens, eight androgens, three progestogens, six glucocorticoids, two mineralocorticoids and two thyroid hormones) in fish and water was develope ...
DOI:
10.1039/c5ay01082a

54. Fish feed as source of potentially allergenic peptides from the fish parasite Anisakis simplex (s.l.)

Author:
C.K. Fæste; A. Levsen; A.H. Lin; N. Larsen; C. Plassen; A. Moen; T. Van Do; E. Egaas
Source:
Animal feed science and technology 2015 v.202 pp. 52-61
ISSN:
0377-8401
Subject:
Anisakis simplex; Danio rerio; Salmo salar; agrochemicals; allergenicity; allergens; crops; enzymes; exposure duration; farm to fork; fish feeds; fish meal; foods; genetically modified plants; human health; hypersensitivity; ingredients; larvae; legumes; liquid chromatography; marine fish; mass spectrometry; meat and bone meal; natural toxicants; nutritive value; parasites; patients; pelagic fish; peptides; plant proteins; pollutants; polyclonal antibodies; poultry industry; prions; risk; safety assessment; veterinary drugs
Abstract:
... The carry-over of certain feed components into animal products can be of concern for human health. The safety assessment of chemical contaminants including natural toxins, agrochemicals, veterinary drugs, and environmental pollutants is a key element of the “farm-to-fork” (“One Health”) approach. The transmissibility of proteinaceous feed constituents such as enzymes, proteins from genetically eng ...
DOI:
10.1016/j.anifeedsci.2015.01.006

55. Metabolism of ginsenosides Rk3 and Rh4 from steamed notoginseng in zebrafish by ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry

Author:
Bin Chen; Yingjie Wei; Dandan Wang; Xiaobin Jia
Source:
Archives of pharmacal research 2015 v.38 no.8 pp. 1468-1476
ISSN:
0253-6269
Subject:
Danio rerio; acetonitrile; biochemical pathways; formic acid; ginsenosides; glucose; ions; liquid chromatography; mass spectrometry; metabolism; metabolites; molecular weight; reference standards
Abstract:
... In this work, the metabolite profiles of ginsenosides Rk₃ and Rh₄ from steamed notoginseng in zebrafish were qualitatively investigated, and their possible metabolic pathways were subsequently proposed. Metabolites of ginsenosides Rk₃ and Rh₄ after exposing to zebrafish for 24 h were identified by Ultraperformance Liquid Chromatography/Quadrupole -Time-of-Flight Mass Spectrometry (UPLC-TOF/MS). Th ...
DOI:
10.1007/s12272-014-0538-7

56. A quantitative HPLC–MS/MS method for studying internal concentrations and toxicokinetics of 34 polar analytes in zebrafish (Danio rerio) embryos

Author:
Stephan Brox; Axel P. Ritter; Eberhard Küster; Thorsten Reemtsma
Source:
Analytical and bioanalytical chemistry 2014 v.406 no.20 pp. 4831-4840
ISSN:
1618-2642
Subject:
2,4-D; Danio rerio; atropine; benzocaine; cimetidine; clofibrate; clofibric acid; developmental stages; liquid chromatography; metazachlor; metribuzin; monitoring; nicotine; pharmacokinetics; phenytoin; tandem mass spectrometry; thiacloprid
Abstract:
... An analytical method using high-performance liquid chromatography–tandem mass spectrometry was developed to determine internal concentrations of 34 test compounds such as pharmaceuticals and pesticides in zebrafish embryos (ZFE), among them, cimetidine, 2,4-dichlorophenoxyacetic acid, metoprolol, atropine and phenytoin. For qualification and quantification, multiple reaction monitoring mode was us ...
DOI:
10.1007/s00216-014-7929-y

57. Influence of the perivitelline space on the quantification of internal concentrations of chemicals in eggs of zebrafish embryos (Danio rerio)

Author:
Stephan Brox; Axel P. Ritter; Eberhard Küster; Thorsten Reemtsma
Source:
Aquatic toxicology 2014 v.157 pp. 134-140
ISSN:
0166-445X
Subject:
Danio rerio; benzocaine; caffeine; chorion; clofibric acid; eggs; liquid chromatography; metribuzin; spectroscopy
Abstract:
... The chorion and the perivitelline space which surround unhatched zebrafish embryos (ZFE, Danio rerio) may affect the determination of internal concentrations of study compounds taken up in early life-stages of ZFE. Internal concentration-time profiles were gathered for benzocaine, caffeine, clofibric acid, metribuzin and phenacetin as study compounds over 96h of exposure starting with ZFE at 4h po ...
DOI:
10.1016/j.aquatox.2014.10.008

58. iTRAQ analysis of gill proteins from the zebrafish (Danio rerio) infected with Aeromonas hydrophila

Author:
Aijun Lü; Xiucai Hu; Yi Wang; Xiaojing Shen; Xue Li; Aihua Zhu; Jun Tian; Qinglei Ming; Zhaojun Feng
Source:
Fish & shellfish immunology 2014 v.36 no.1 pp. 229-239
ISSN:
1050-4648
Subject:
Aeromonas hydrophila; Danio rerio; Escherichia coli; bacterial infections; coagulation; complement; creatine kinase; defense mechanisms; fish; gene expression regulation; genes; gills; glyceraldehyde-3-phosphate dehydrogenase; histones; immune response; liquid chromatography; microarray technology; mucosa; pathogens; peroxiredoxin; phagosomes; protein synthesis; proteome; tandem mass spectrometry
Abstract:
... The gills are large mucosal surfaces and very important portals for pathogen entry in fish. The aim of this study was to determine the gill immune response at the protein levels, the differential proteomes of the zebrafish gill response to Aeromonas hydrophila infection were identified with isobaric tags for relative and absolute quantitation (iTRAQ) labeling followed by liquid chromatography-tand ...
DOI:
10.1016/j.fsi.2013.11.007

59. Bioaccumulation and transformation of methylmercury and selenite using zebrafish (Danio Rerio) larvae as a model

Author:
S. Cuello; J. Sanz-Landaluze; Y. Madrid; J. Guinea; C. Cámara
Source:
Talanta 2012 v.89 no. pp. 169-177
ISSN:
0039-9140
Subject:
Danio rerio; bioaccumulation factor; biotransformation; exposure duration; larvae; lethal concentration 50; liquid chromatography; mass spectrometry; methylmercury compounds; models; solvents
Abstract:
... Bioaccumulation and possible transformation of methylmercury and selenite has been checked on a 72h-cycle of bioaccumulation and depuration using larvae from zebrafish. The larvae were exposed to methylmercury and selenite at concentrations of 1% and 0.1% of their LC₅₀ values. Quantitative extraction of methylmercury and selenite from exposed larvae was achieved by using ultrasonic probe-assisted ...
DOI:
10.1016/j.talanta.2011.12.011

60. Proteome profile of zebrafish kidney

Author:
Sandeep Saxena; Sachin K. Singh; Mula G. Meena Lakshmi; Vuppalapaty Meghah; Curam S. Sundaram; Cherukuvada V. Brahmendra Swamy; Mohammed M. Idris
Source:
Journal of proteomics 2011 v.74 no.12 pp. 2937-2947
ISSN:
1874-3919
Subject:
Danio rerio; biomarkers; cytoskeleton; data collection; desorption; gels; ionization; kidneys; liquid chromatography; proteins; proteome; proteomics; two-dimensional gel electrophoresis
Abstract:
... The most imperative organ, kidney has been widely studied in zebrafish for its simplified structures and development. Understanding the proteomic component of kidney might lead to a better insight for understanding the structural and functional complexity of kidney. In this study we have analyzed the proteome profile of the zebrafish kidney based on gel based proteome mapping techniques involving ...
DOI:
10.1016/j.jprot.2011.07.021
PubMed:
21839869

61. SILAC zebrafish for quantitative analysis of protein turnover and tissue regeneration

Author:
Ann Westman-Brinkmalm; Alexandra Abramsson; Josef Pannee; Chen Gang; Mikael K. Gustavsson; Malin von Otter; Kaj Blennow; Gunnar Brinkmalm; Hermann Heumann; Henrik Zetterberg
Source:
Journal of proteomics 2011 v.75 no.2 pp. 425-434
ISSN:
1874-3919
Subject:
Danio rerio; adults; amputation; animal models; brain; cell culture; diet; heart; heart failure; human diseases; isotope labeling; liquid chromatography; liver; lysine; neurodegenerative diseases; peptides; protein synthesis; proteins; quantitative analysis; respiratory tract diseases; stable isotopes; tandem mass spectrometry; tissue repair
Abstract:
... Defective tissue regeneration is thought to contribute to several human diseases, including neurodegenerative disorders, heart failure and various lung diseases. Boosting the regenerative capacity has been suggested a possible therapeutic approach. Methods to metabolically label newly synthesized proteins in vivo with stable isotopic forms of amino acids holds promise for the study of protein turn ...
DOI:
10.1016/j.jprot.2011.08.008
PubMed:
21890006

62. Iodine species and the endocrine system: thyroid hormone levels in adult Danio rerio and developing Xenopus laevis

Author:
R. Simon; J. Tietge; B. Michalke; S. Degitz; K.-W. Schramm
Source:
Analytical and bioanalytical chemistry 2002 v.372 no.3 pp. 481-485
ISSN:
1618-2642
Subject:
Danio rerio; Xenopus laevis; adults; blood serum; endocrine system; females; humans; iodides; iodine; liquid chromatography; males; mass spectrometry; tadpoles; thyroid hormones
Abstract:
... Recently a new approach for the analysis of iodinated organic species in human serum has been developed using liquid chromatography–inductively coupled plasma–mass spectrometry (LC–ICP–MS). This method enables quantification of iodide, T₄and T₃, as well as reverse T₃(rT₃) and the synthetic precursors of TH, monoiodotyrosine (MIT), and diiodotyrosine (DIT) in a single injection. In this work, the L ...
DOI:
10.1007/s00216-001-1211-9