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agricultural watersheds; water pollution; Escherichia coli; Enterococcus; indicator species; feces; livestock; wild animals; population density; surface water; streams; Georgia
Abstract:
... The Upper Oconee watershed of Georgia is typical of the Piedmont and water quality is a key issue in the urbanization of this historically agricultural region. Fecal indicators may be found in higher numbers than regulatory targets and agricultural non-point sources are sometimes implicated. We enumerated E. coli and enterococci bacteria at 18 sites for 3 years (2200 observations) within the Upper ...
... A plating procedure simulating conditions in ripening Cheddar cheese was used to isolate microorganisms in young cheese that grow at ordinary ripening temperatures. Forty-one commercial cheese samples, most 7 days old, were obtained from ten Iowa manufacturing plants. Samples were plated in special Trypticase-soy agar, covered with 50 ml of 1.5% (w/v) agar, and incubated at 7.2 C for 3 months. Fol ...
... Four types of luncheon meats, bologna, chicken loaf, ham, and macaroni cheese, each manufactured by four different companies, were purchased from four major retail outlets in Ontario over a period of 16 weeks during the summer of 1975. Bacterial evaluation included determination of total aerobic plate count, coliforms, Escherichia coli, Staphylococcus aureus, Clostridium perfringens, salmonellae, ...
... Five lots of fish were examined microbiologically for aerobic plate count at 10 and 30 C. coliform and enterococcus counts, presence of coagulase-positive Staphylococcus aureus and genera of bacteria appearing on the aerobic plates. After initial analyses. fish were stored in ice, held there for a week and then aerobic plate counts and isolation of colonies for identification as to genus were done ...
Enterococcus; Food and Drug Administration; Salmonella; Vibrio parahaemolyticus; coagulase positive staphylococci; coliform bacteria; crab meat; crabs; enteropathogens; fecal bacteria; microbiological quality; oysters; scallops; seasonal variation; shellfish; shrimp; North Carolina
Abstract:
... Microbiological analyses of 716 seafood samples over a 3-year period revealed that the microbiological quality of fresh seafood in North Carolina was generally acceptable. The mean aerobic counts (APC) and fecal coliform counts were low as was the occurrence of enteric pathogens, except for Vibrio parahaemolyticus and coagulase-positive staphylococci. Salmonella species were isolated from three sa ...
... The phylogenetic interrelationships of the genera Pediococcus and Aerococcus were investigated using reverse transcriptase sequencing of 16S rRNA. The genus Pediococcus was found to be phylogenetically heterogeneous. The four species P. acidilactici, P. damnosus, P. parvulus and P. pentosaceus formed a phylogenetically distinct group. Within this pediococcal cluster, P. acidilactici was closely re ...
... A replicated, multiyear field study was conducted to assess the reduction of N, P and microbial indicators by a recirculating sand filter (RSF) and buried multilayer sand filter patterned after the RUCK filter. The RSF's received 38 L m⁻¹ d⁻¹ of septic tank effluent, while the buried sand filters were loaded at 76 L m⁻² d⁻¹. The RSF's had significantly greater reductions of N and P (21 and 31%, re ...
... Balance studies in germ‐free (GF) and conventional (CV) rats have shown that in‐vivo breakdown of amylomaize starch depends on endogenous and bacterial enzymes. In GF rats, one‐third of amylomaize starch is resistant to endogeneous digestion, whereas in CV rats starch is totally degraded, In order to study the contribution of endogenous and bacterial enzymes, the in‐vivo breakdown of amylomaize st ...
roosters; semen; microbial contamination; plate count; culture media
Abstract:
... Three experiments were conducted to determine the bacteriological quality of chicken semen. Semen was collected from donor males, diluted, and surface inoculated onto seven different bacteriological media, from which randomly selected colonies were identified. Bacterial counts in semen averaged 5.14 log10 cfu/mL. Tryptic soy agar (TSA) was the best medium for the isolation of Gram-positive bacteri ...
... Studies were conducted to identify the bacteria responsible for spoilage of fresh broiler chicken carcasses and to characterize the off-odors these bacteria produce. Broiler carcasses were collected from processing plants in the northeast Georgia area, the southeastern U.S., Arkansas, California, and North Carolina. The carcasses were allowed to spoil under controlled conditions at 3 C and spoilag ...
... In a colony of severe combined immunodeficiency (SCID) mice conspicuously altered behavioural characteristics were observed: hunched position, apathy, dullness, short breath, bristled fur, emaciation, circling movements around their longitudinal axis and oblique head posture. This was most common in pregnant and lactating animals and also observed in 4 mice after experimental treatment. Pseudomona ...
... BLIS 213, is a bacteriocin-like inhibitory substance produced by Carnobacterium piscicola 213. It is active against Carnobacterium, Enterococcus and Listeria spp. No activity was observed against tested Lactobacillus, Lactococcus, Leuconostoc and Pediococcus strains, nor against Gram-negative bacteria. The BLIS 213 activity was inactivated by several proteolytic enzymes. It was heat resistant (121 ...
dietary fat; short chain fatty acids; intestinal mucosa; azoxymethane; nutrient intake; intestinal microorganisms; cecum; bile acids; long chain fatty acids; water content; feces composition; rats; enzyme activity; experimental diets; digesta; water; pH; sterols; alkaline phosphatase
Abstract:
... To shed light on the association of dietary fat with the development of colon cancer, we studied the ability of azoxymethane (AOM) to induce aberrant crypt foci (ACF) and biochemical changes in rats fed high- or normal-fat diets. Six-week-old male Fischer 344 rats were placed on a high-fat 17% (wt/wt) soybean oil + 15% (wt/wt) beef tallow]or a normal-fat (7% soybean oil, AIN-93G) diet. Rats fed ea ...
... Raw milk from 27 farms was sampled over 6 months for listerias, salmonellas, Yersinia enterocolitica and campylobacters. Total bacterial counts and somatic cell counts were measured. Lactococci, lactobacilli, dextran-producing leuconostocs, Brevibacterium linens, yeasts and moulds, Staphylococcus aureus and other Micrococcaceae, Pseudomonas, coliforms, Escherichia coli, enterococci, Clostridium pe ...
... Recent outbreaks of cryptosporidiosis and reports of other newly described para-sitic diseases associated with drinking water transmission prompted a reevaluation of source water monitoring criteria for public health protection. The field of microbial indicators was reviewed and each candidate sentinel evaluated in terms of its sensitivity, specificity, and technical feasibility. In addition, a cl ...
... Two hundred and seventy-seven Enterococcus isolates representing the predominating enterococcal flora of retailed chicken and pork were identified by phenotypical features, and by random amplified polymorphic DNA. The resistance to nine different antibiotics was determined. Enterococcus faecium was the most frequently occurring species in both Swedish and Danish chicken. Enterococcus faecalis and ...
... Resistance to glycopeptide antibiotics in enterococci results from the synthesis of peptidoglycan precursors with low affinity for these antibiotics. The resistance proteins are encoded on transposons in VanA and VanB type enterococci and are involved in regulation, synthesis of new resistant precursors and elimination of wild-type sensitive precursors by hydrolysis of D-alanyl-D-alanine (D,D-pept ...
dogs; cystitis; bladder; congenital abnormalities; Escherichia coli; Enterococcus; case studies
Abstract:
... A 14‐month‐old, male German shepherd dog was evaluated for chronic, recurrent Escherichia coli urinary tract infection. An initial diagnosis of emphysematous cystitis was made, which resolved with appropriate antibacterial therapy. The urinary tract infection, however, did not resolve and on further investigation a bladder trigone diverticulum was evident, thought to be congenital in origin. This ...
... The BIOMIC is a computerized system used to calculate the minimal inhibitory concentration (MIC) of an antimicrobial agent from a zone of inhibition generated by a disk diffusion test. This system was developed using bacterial pathogens of human origin. This study investigated the use of the BIOMIC system for determining MICs for bacterial pathogens from animals. The MICs generated by the BIOMIC s ...
... Low-affinity penicillin-binding proteins (PBPs), which participate in the β-lactam resistance of several pathogenic bacteria, have different origins. Natural transformation and recombination events with DNA acquired from neighbouring intrinsically resistant organisms are responsible for the appearance of mosaic genes encoding two or three low-affinity PBPs in highly resistant strains of transforma ...
Enterococcus; Escherichia coli; United States Environmental Protection Agency; coastal water; coliform bacteria; edaphic factors; experimental design; humidity; indicator species; islands; rain; runoff; soil; soil bacteria; soil sampling; streams; temperature; washing; water analysis; water quality; water quality standards; Guam; Hawaii
Abstract:
... We have previously documented that faecal indicator bacteria (Escherichia coli, faecal coliform, enterococci) recommended by the U.S. Environmental Protection Agency (USEPA) to establish recreational water quality standards are naturally found in high concentrations in the surface and subsurface of soils in Hawaii. Rain, the source of all streams in Hawaii, washes the soil sources of faecal bacter ...
... From human feces two phenotypically different types of bacteria were isolated on quercetin-3-glucoside as carbon and energy source. Isolates of one type were identified as strains of Enterococcus casseliflavus. They utilized the sugar moiety of the glycoside, but did not degrade the aglycon further. The sugar moiety (4 mM) was fermented to 5.5 +/- 2.1 mM formate, 2.1 +/- 0.7 mM acetate, 1.6 +/- 0. ...
... Two bacteriocinogenic strains of Pediococcus parvulus and one bacteriocinogenic Enterococcus mundtii strain were evaluated for their potential to control the growth of Listeria monocytogenes on refrigerated, modified atmosphere (MA) stored mungbean sprouts. These three strains, which were isolated from minimally‐processed vegetables, were shown to grow in culture broth at 4, 8, 15 and 30 °C. Howev ...
... Thermophilic aerobic digestion (TAD), or liquid composting, is a versatile new process for the treatment and stabilization of high strength wastes of liquid or, perhaps more importantly, slurry consistency. The pattern of inactivation of various pathogenic and indicator organisms was studied using batch digestions under conditions that may be expected to be found in full-scale TAD processes. Rapid ...
... Two experiments were conducted to examine the effects of different fat types, i.e., soybean oil (S) and beef tallow (T), in rye-based broiler diets, either unsupplemented (-) or supplemented (+) with xylanase (Avizyme 1300 at 1 g/kg diet), on selected bacterial groups adhering to the epithelium of crop, duodenum, jejunum, and ileum (Experiment 1, 16 d of age), on mean retention time (MRT) of diges ...
Enterococcus; HACCP; air temperature; cold storage; cooling; critical control points; heat treatment; refrigeration; spoilage; yeasts
Abstract:
... In the commercial processing of croquettes a 80 °C heat treatment for 30 min is applied to the raw materials to make a semi-solid paste called „bechamel”. Since the heat treatment is not that of sterilization, some micro-organisms surviving it may proliferate in the next steps, which could introduce two microbial hazards; the presence of pathogenic bacteria and croquettes spoilage. To evaluate mic ...
Enterococcus; HACCP; aerobes; air temperature; bacteria; biological hazards; cold storage; cooking; cooling; critical control points; heat treatment; raw materials; refrigeration; spoilage; yeasts
Abstract:
... In the commercial processing of croquettes a 80 °C heat treatment for 30 min is applied to the raw materials to make a semi-solid paste called „bechamel”. Since the heat treatment is not that of sterilization, some micro-organisms surviving it may proliferate in the next steps, which could introduce two microbial hazards; the presence of pathogenic bacteria and croquettes spoilage. To evaluate mic ...
... Avoparcin was used as a feed additive in Norwegian broiler and turkey production from 1986 until 1995. It was banned due to the selection of VanA‐type vancomycin‐resistant enterococci (VRE) in animal husbandry and to reduce the potential for human exposure to VRE. The aim of the present study was to investigate the prevalence of VRE carriage in Norwegian poultry farmers and their poultry three yea ...
... Control of the foodborne pathogens Escherichia coli O157:H7, Salmonella typhimurium, Staphylococcus aureus, and Listeria monocytogenes during sufu fermentation was evaluated. Before fermentation, pathogens were inoculated onto tofu (substrate for sufu) at 5 log cfu/g or 3 log cfu/g, and starter culture (Actinomucor elegans) was inoculated at 3 log cfu/g. After 2 days of fermentation at 30 degrees ...
... Objective: In intensive care patients, enteral feeding requires sterile feedings because of infectious complications and adequate supplements to meet nutritional needs. Heretofore, prepacked, large-volume formula containers were developed, but bacterial contamination occurred in 4% to 15%. Our objective was to investigate the microbial contamination rate of 1-L feeding bottles and newly designed a ...
... Aim: The two bacterial species, Eubacterium ramulus and Enterococcus casseliflavus, which had previously been isolated from human faeces using the flavonoid quercetin-3-glucoside as the growth substrate, were tested for their ability to utilize this compound in vivo. Methods and Results: Germ-free rats were associated with Eu. ramulus and subsequently with Ent. casseliflavus and vice versa. Identi ...
... A feeding experiment was carried out over 42 d with four groups of broiler chickens fed experimental diets formulated to provide no supplementation, 20 mg zinc bacitracin, 60 mg salinomycin, or both feed additives in combination. During the fifth week of the experiment, four chickens from each pen were killed, and the contents of gizzard, duodenum, jejunum, ileum, ceca, and rectum were separately ...
... Enterococcus gallinarum strain 012, isolated from the duodenum of ostrich, produced enterocin 012 which is active against Ent. faecalis, Lactobacillus acidophilus, Lact. sake, Listeria innocua, Propionibacterium acidipropionici, Propionibacterium sp., Clostridium perfringens, Pseudomonas aeruginosa and Salmonella typhimurium. One of the four pathogenic strains of Escherichia coli isolated from the ...
pancreas; lungs; pancreatitis; kittens; liver; inflammation; Enterococcus; epithelium; feces; disease transmission; case studies; bile ducts; small intestine; mortality; infection
Abstract:
... A 2-month-old female Persian cat that had been showing episodes of anorexia and diarrhea for the previous 4 weeks was presented in shock and died 2 days later. Numerous Gram-positive cocci were located along the brush border of small intestinal villi, without significant inflammatory infiltration. Similar bacteria were present within hepatic bile ducts and pancreatic ducts and were associated with ...
... Strains of Lactobacillus reuteri and Lact. rhamnosus are used as probiotics in man and animal. The aim of this study was to determine whether the glycopeptide resistance in these lactobacilli has a similar genetic basis as in enterococci. Five Lact. reuteri strains and one Lact. rhamnosus, as well as four Enterococcus control strains, were probed for the vanA gene cluster, the vanB gene and the va ...
... A new antimicrobial peptide, hadrurin, was isolated from the venom of the Mexican scorpion Hadrurus aztecus, by gel filtration on a Sephadex Gâ50 column, followed by high performance liquid chromatography. It is a basic peptide composed of 41 aminoâacid residues with a molecular mass of 4436âDa, and contains no cysteines. A model of the threeâdimensional folding of hadrurin is compatible w ...
... The diversity and population densities of facultative anaerobic bacteria with the capacity to hydrate oleic acid and linoleic acid in the rumen of sheep and dairy cows were determined. The screening of representative colonies, from rumen fluid plated aerobically on a range of agar media, revealed that sheep rumen fluid contained hydration-positive strains of Streptococcus, Staphylococcus, Enteroco ...
... A bacteriocin-producing Lactobacillus gasseri strain, KT7, was isolated from infant faeces. The supernatant fluid showed inhibitory activity not only against some lactic acid bacteria but also, against some pathogenic and food-spoilage species, including Clostridium, Listeria and Enterococcus. An antimicrobial peptide designated gassericin KT7 was isolated from Lactobacillus gasseri KT7. It was pu ...
Enterococcus; Escherichia coli; Listeria monocytogenes; Salmonella; food safety; hygiene; raw materials; Mediterranean region
Abstract:
... The aim of this work was to study in six different types of European dry-sausages (of the Mediterranean area) the ocurrence of contaminant biota: enterobacteria, coliforms, E. coli, enterococci, sulphite-reducing clostridia, Salmonella-Shigella and Listeria monocytogenes, in the course of the ripening process. A total of 162 samples were analysed at different stages of the elaboration process. The ...
Enterococcus; Escherichia coli; Listeria monocytogenes; Salmonella; coliform bacteria; food safety; raw materials; ripening; yeasts; Mediterranean region
Abstract:
... The aim of this work was to study in six different types of European dry-sausages (of the Mediterranean area) the ocurrence of contaminant biota: enterobacteria, coliforms, E. coli, enterococci, sulphite-reducing clostridia, Salmonella-Shigella and Listeria monocytogenes, in the course of the ripening process. A total of 162 samples were analysed at different stages of the elaboration process. The ...
... The ability of different enteric pathogens and coliforms to trigger agglutination of yeast cells (Sacharomyces cerevisiae, NCYC 1026) and a yeast cell wall preparation (MOS) was examined. Five of seven strains of Escherichia coli and 7 of 10 strains of Salmonella typhimurium and Salmonella enteritidis agglutinated MOS and Sac. cerevisiae cells. Strains of Salmonella choleraesuis, Salmonella pullor ...
... This article describes a microbiological study carried out on lacon, a dry-cured meat product made in the north-west of Spain from the fore extremity of pig. Using classical methods, aerobic mesophilic flora, salt-tolerant flora, lactic acid bacteria, Enterobacteriaceae, enterococci, moulds and yeasts were enumerated, some physicochemical parameters (pH, a(w) and moisture and NaCl contents) were d ...
Enterococcus; Lactobacillus; Lactococcus; Leuconostoc; Pediococcus; Streptococcus; Weissella; denaturing gradient gel electrophoresis; lactic acid bacteria; ribosomal DNA
Abstract:
... Separation of amplified V3 region from 16S rDNA by denaturing gradient gel electrophoresis (DGGE) was tested as a tool for differentiation of lactic acid bacteria commonly isolated from food. Variable V3 regions of 21 reference strains and 34 wild strains referred to species belonging to the genera Pediococcus, Enterococcus, Lactococcus, Lactobacillus, Leuconostoc, Weissella, and Streptococcus wer ...
... G. MAURIELLO, L. MOIO, G. MOSCHETTI, P. PIOMBINO, F. ADDEO AND S. COPPOLA. 2001. Aims: Seventy‐eight strains of lactic acid bacteria belonging to five genera and showing six different phenotype combinations of Lac (lactose fermentation), Prt (proteolytic activity) and Cit (citrate degradation) characters were investigated for their main flavouring properties with the aim to detect variability amon ...
... Aims: Enterococcus isolates from forage grass were subjected to taxonomical investigations and tested for antibiotic resistance. Methods and Results: The identification procedure included phenotypic characterizations, restriction analyses of polymerase chain reaction-amplified 16S rDNA, whole-cell protein profile analyses and 16S rDNA sequence analyses. Agar diffusion tests were performed to detec ...
... Poultry are increasingly being associated with carriage of multiresistant organisms that may cause disease in humans. Among these organisms are the enterococci, now regarded as a common cause of hospital-acquired infections. The use of antimicrobials for growth promotion in poultry production environments may facilitate the dissemination of resistance to Enterococcus spp. that have the potential t ...
... Enterococci and staphylococci, isolates from faecal samples of 46 different animals such as deer, chamois, European bison, zebra, camel, antelope, gazelle, horse, and piglets were treated by nisin (concentration 1 mg/ml). Only two strains (SX38 and EA163), isolates from the faeces of deer were not inhibited by nisin under in vitro conditions. It means 97.4% of target isolates were inhibi ...
... Aims: To examine the presence of lactic acid bacteria (LAB: enterococci, streptococci and lactobacilli) in the pigeon crop. Methods and Results: The crops of 10 pigeons were sampled and inoculated on agar plates for isolation of streptococci, enterococci and lactobacilli. The isolates were identified using tDNA-PCR. Lactobacillus agilis, a species described in 1981 from municipal sewage, was the d ...
... 118 strains of heterotrophic microorganisms were isolated from goat cheese produced domestically in the IV Region of Northern Chile (Serene, Ovalle, and Illapel) and sold in supermarkets in Valparaíso, Chile. The results of 89 phenotypic tests were numerically analyzed against 17 reference strains, using the simple matching coefficient (SSM). Thirteen phena were found at a 78% similarity level. Fi ...
... Aims: To investigate the spatial and temporal dynamics of enterococci colonizing forage grass and their ability to produce bacteriocins. Methods and Results: Enterococci could be detected on above-ground plant parts throughout the growing season, with high continuity but low cell numbers (2.60 x 10(1)-6.16 x 10(4) cfu g(-1) fresh matter). A total of 750 strains were isolated and identified by thei ...
... Antibiotic resistance analysis (ARA) was used to determine if enterococci of human origin were present in a stream (Spout Run) that passes through a rural nonsewered community (Millwood, VA). Millwood consists of 82 homes, all served by individual septic systems, and Spout Run drains a 5800-ha karst topography watershed that contains large populations of livestock and wildlife. Periodic monitoring ...
Enterococcus casseliflavus; Helix aspersa; bacteria; gardens; intestines; nucleic acid hybridization; ribosomal DNA; snails; soil; species identification; transfer RNA
Abstract:
... Aims: Enterococci associated with garden snails (Helix aspersa) were studied in order to obtain reliable species identification and characterization. Methods and Results: Twelve yellow-pigmented and motile enterococci, isolated from the intestines of garden snails, were phenotypically close to Enterococcus casseliflavus, but they showed certain unusual biochemical characteristics. tRNA intergenic ...
terpenoids; chemical composition; antibacterial properties; Candida albicans; Escherichia coli; essential oils; antifungal properties; oxidation; Enterococcus hirae; chemical constituents of plants; roots; shoots
Abstract:
... The volatile oils of the aerial parts and roots from a pasture plant, Elionurus elegans, were studied by GC-MS analyses. Both organs studied contained only terpenic constituents. The main components found in the extract essential oils of the aerial parts were campherenone (43.0%), caryophyllene oxide (4.9%), and bisabolone (4.9%), whereas those found in the root essential oils were campherenone (3 ...
... Aims: To identify the streptococcal and enterococcal components of the microflora from pigeon intestines to species level by tDNA-intergenic polymerase chain reaction. Methods and Results: Gram-positive coccal strains were isolated from the intestines or faeces of 50 different pigeons, all from different lofts, on Columbia CNA blood agar. Sodium azide-sensitive enterococci were found to constitute ...
... Because of the rapid development and spread of antimicrobial resistance it is important that a system be established to monitor antimicrobial resistance in pathogenic zoonotic and commensal bacteria of animal origin. Susceptibility testing of bacteria from carcasses and different samples of animal origin has been carried out in veterinary institutes for a long time but by an inconsistent methodolo ...
... Because of the rapid development and spread of antimicrobial resistance it is important that a system be established to monitor antimicrobial resistance in pathogenic zoonotic and commensal bacteria of animal origin. Susceptibility testing of bacteria from carcasses and different samples of animal origin has been carried out in veterinary institutes for a long time but by an inconsistent methodolo ...
... This study first investigates the effects of mash diet, or mash supplemented with either 2.5% mannoseoligosaccharide (MOS) or palm kernel meal (PKM), on the microflora of the hen caecal contents. Second, it investigates the effect of caecal contents of hens (HCC) fed mash or mash supplemented with MOS or PKM on the major microflora groups of chicks, and their inhibitory effect on Salmonella enteri ...
... Aims: To compare different tests in the identification of Enterococcus durans, E. hirae and E. villorum strains. These bacteria belong to the E. faecium species group and are phylogenetically closely related, as evidenced by 16S rRNA sequence homologies of over 98.8%. Methods and Results: Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis of whole-cell protein, tRNA interpacer pol ...
... Excessive application of swine manure on agricultural lands is likely to increase water pollution. Potential impacts of swine manure management on bacterial contamination in subsurface drainage are often difficult to assess in the field. In this study, leachate from intact 20-cm (8-in.) diameter, 30-cm (12-in.) long soil columns receiving simulated fall and spring manure applications at 168 kg N/h ...
... Molecular approaches were used to examine the genetic diversity of bacteria associated with the gut wall of the terrestrial isopod Porcellio scaber and to determine whether an autochthonous microflora exists in the P. scaber hindgut. 16S ribosomal genes were amplified from the total DNA isolated from thoroughly washed papillate regions of the hindgut, where the highest concentrations of bacteria a ...
Enterococcus; Lactococcus; cheese milk; cheeses; dairy industry; ewe milk; genetic variation; genotype; manufacturing; microorganisms; plasmids; protected designation of origin; pulsed-field gel electrophoresis; raw milk; ripening; starter cultures
Abstract:
... Aims: To assess the intraspecific genetic diversity of lactococci and enterococci isolated from 24-h, 1- and 2-month-old home-made Pecorino Sardo ewes' milk cheese. Methods and Results: Two molecular techniques, plasmid profiling and pulsed-field gel electrophoresis, were used in order to type the isolates at strain level. The present study revealed that the lactococcal and enterococcal microbial ...
... The pharmacodynamic properties of a new veterinary fluoroquinolone antimicrobial agent, ibafloxacin, were evaluated. Minimal inhibitory concentrations (MIC), time-kill kinetics, postantibiotic effect (PAE) and postantibiotic subminimal inhibitory concentration effects (PA-SME) were determined against pathogenic canine Gram-negative and Gram-positive bacterial isolates from dermal, respiratory and ...
... A range of probiotic and other intestinal bacteria were examined for their ability to ferment the dietary fibre carbohydrates β-glucan, xylan, xylo-oligosaccharides (XOS) and arabinoxylan. β-Glucan was fermented by Bacteroides spp and Clostridium beijerinckii but was not fermented by lactobacilli, bifidobacteria, enterococci or Escherichia coli. Unsubstituted xylan was not fermented by any of the ...
... A strain of Enterococcus faecium isolated from Bulgarian yellow cheese “kashkaval” produced a bacteriocin-like substance named enterococcin A 2000. The antibacterial substance had a low molar mass (<2 kDa), was relatively stable toward heat but was sensitive to selected proteolytic enzymes. It was active against Gram-positive bacteria including enterococci, such as Listeria, Bacillus and Streptoco ...
... The purpose of this review article on the lactic acid bacteria grew from an early curiosity and a desire to convey and impart the broad scope of literary information on their functions as starter cultures, in the manufacture of fermentation products such as dairy products and alcoholic beverages, as well as their contribution to better health. This review article is an attempt to empower the reade ...
... Aims: To investigate the antagonistic activity of two lactic acid strains against the spoilage microflora in cooked cured meat products, vacuum or modified atmosphere packed at 4 degrees C and to determine the inhibitory capacity of their bacteriocins. Methods and Results: Frankfurter-type sausages and sliced cooked cured pork shoulder were inoculated with Leuconostoc mesenteroides L124 and Lactob ...
... Aims: The generation of data of real relevance to the purported risks of DNA transfer from food-borne genetically modified microorganisms (GMMOs) using the human biota associated (HBA) rat model. Plasmid transfer between Lactococcus lactis strains and between donor strains and human gut bacteria was monitored. Methods and Results: Transfer of the recombinant plasmid pCK1 and/or the promiscuous non ...
... Aims: A survey was conducted on the ecological distribution of bacteriocin-producing lactic acid bacteria in Miso-pastes, a typical fermented food in Japan. Method and Results: Nine Miso-pastes were sampled for isolation of bacteriocin-producers. Almost all isolated enterococcal strains produced bacteriocins but no isolated tetragenococci did so. The bacteriocin-producing isolates were divided int ...
Enterococcus; Lactobacillus; agar; bile; control methods; feed additives; feeds; plate count; probiotics; yeasts; Europe
Abstract:
... Aims: Validation of an enumeration method to be used as an official control method in the framework of Council Directive 70/524/EEC for probiotic enterococci used as feed additives. Methods and Results: Twenty laboratories in 12 European countries carried out a collaborative study. A plate count method using bile esculin azide (BEA) agar was used. Precision data in terms of repeatability (r) and r ...
... Aims: To determine the sensitivity to honey of Gram-positive cocci of clinical significance in wounds and demonstrate that inhibition is not exclusively due to osmotic effects. Methods and Results: Eighteen strains of methicillin-resistant Staphylococcus aureus and seven strains of vancomycin-sensitive enterococci were isolated from infected wounds and 20 strains of vancomycin-resistant enterococc ...