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... Daqu, an indispensable starter, significantly affects the quality and yield of Chinese baijiu. To explore the bioturbation effect of space mutation and biofortification on strong-flavor Daqu, the microbial community, physicochemical properties and overall metabolic profile were investigated. Lactobacillus and Aspergillus were significantly reduced after bioturbation, while seven genera (e.g., Baci ...
Enterococcus; Oplegnathus; antioxidant activity; aquaculture; blood serum; body weight changes; captive animals; complement; fish; globulins; glutathione peroxidase; growth performance; hematology; immune response; intestinal microorganisms; land management; lysozyme; models; net pens; phagocytosis; protein content; respiratory burst; somatotropin; specific growth rate; superoxide dismutase
Abstract:
... The land (recirculating aquaculture system, RAS)-sea (offshore aquaculture net pen system, OANPS) relay alternate culture is a new model for fish farming based on seasonal changes. However, the characteristics of fish reared by land-sea relay farming are unclear. In the current study, the growth performance, hematology parameters, antioxidant capacity, immune response, and intestinal microbiota of ...
... Maillard reaction products (MRPs) play pivotal roles in gut health by affecting the microbiome-host interactions. This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation. The pH decreased sharply in the first 12 h and the ...
... Enzymatically pre-treated sugarcane bagasse (SCB) was used as substrate for sequential production of hydrogen (H₂) and methane (CH₄). In stage I, SCB was used by autochthonous bacteria, such as Enterococcus (42.4 % relative abundance) and Paraclostridium (16.8 %) for H₂ production (166.8 mL H₂/L), while the metabolites accumulated at the end of this stage (9140.5 mg HAc/L) were used by allochthono ...
... This study explored the effects of H₂O₂ on Cyanobacteria and non-target microbes using fluorometry, microscopy, flow cytometry, and high throughput DNA sequencing of the 16S rRNA gene during a series of mesocosm and whole-ecosystem experiments in a eutrophic pond in NY, USA. The addition of H₂O₂ (8 mg/L) significantly reduced Cyanobacteria concentrations during a majority of experiments (66%; 6 of ...
... The correlation of antioxidant activity and prebiotic effect about oat phenolic compounds was invested, while there exists limited studies. Free and bound phenolic compounds were separated from ethyl acetate, n-butanol and aqueous fractions. Fluorescence in situ hybridization was used to investigate gut microbiota of in vitro fermentation samples. The results showed that ethyl acetate fraction pos ...
... Enzyme activity plays an important role in the functioning of aquatic ecosystems. It is sensitive to changes in environmental conditions such as pH, temperature, and nutrient concentration. The objective of this work was to determine the acid phosphatase activity (AcPA) in the Almendares and San Juan rivers (western Cuba) and its relationship with physicochemical and microbiological indicators. Fo ...
... In aquaculture, essential oils are considered promising supplements that can improve productive performance and modulate the fish's microbiota. Here we investigate how a blend of essential oils (carvacrol + thymol + cinnamaldehyde acid) affects the productive performance and the intestinal microbiota of juvenile Nile tilapia (Oreochromis niloticus, Thai strain). All fish were randomly distributed ...
... Exposure to bioaerosols causes infection, over-sensitivity, respiratory, and lung diseases. This study was conducted at Sanandaj wastewater treatment plant in three seasons of winter, spring and summer to investigate the type and density of bio-aerosols. Sampling was performed using a sampling pump with a discharge rate of 28.3 L/min at a height of 1.5 m above the ground; the samples were taken fr ...
... The gut microbiota consists a diverse and complex ecosystem that is involved in beneficial functions as well as potentially harmful conditions for human. Blastocystis sp. is a common parasite of the digestive tract of animals and humans; however, limited data is available concerning the association of asymptomatic Blastocystis infection and gut bacteria composition. Hence, in this cross-sectional ...
... BACKGROUND: Rabbit coccidiosis is a major disease caused by various Eimeria species and causes enormous economic losses to the rabbit industry. Coccidia infection has a wide impact on the gut microbiota and intestinal biochemical equilibrium. In the present study, we established a model of Eimeria intestinalis infection in rabbits to evaluate the jejunal microbiota and fecal metabolite profiles. M ...
... The role of oral microbiota in viral encephalitis and/or viral meningitis (VEVM) remains unclear. In this hospital-based, frequency-matched study, children with clinically diagnosed VEVM (n = 68) and those with other diseases (controls, n = 68) were recruited. Their oral swab samples were collected and the oral microbiota was profiled using 16S rRNA gene sequencing. The oral microbiota of children ...
Actinobacteria; Enterococcus; Lactococcus; Paenibacillus; Proteobacteria; Sporosarcina; altitude; atmospheric pressure; biodegradation; biosynthesis; body temperature; cecum; chickens; cold; energy intake; genes; ileum; intestinal microorganisms; microbiome; oxygen; signal transduction; xenobiotics
Abstract:
... Low oxygen levels and extremely cold weather in high-altitude environments requires more energy intake to maintain body temperature in animals. However, little is known about the characteristics of cecal and ileac microbiota in Tibetan chicken and how the high and low altitude environments affect the gut microbiota communities in Tibetan chicken. In the present study, In the present study, Tibetan ...
... This study aimed to investigate the presence of eight virulence genes (ace, asa1, esp, efaA, gelE, cylA, agg, fsr) in Enterococcus from a variety of animals and to explore the drug resistance and pathogenicity. This could provide a theoretical basis for clinical treatment of Enterococcus infections. Anal swabs from pigs, chickens, cattle, and dogs in farms and pet hospitals were collected for Ente ...
... Knowing the antibiotic resistance profile of pathogenic bacteria is essential to finding an appropriate remedy for these issues by sourcing antibacterial agents from plants. The present study exposes the bactericidal potential of different leaf extracts of Blepharis maderaspatensis and Ziziphus oenoplia against multi drug resistant bacterial strains enumerated from pus specimens at a secondary car ...
Acinetobacter; Enterococcus; Escherichia coli; One Health initiative; Pasteurella; Staphylococcus; Stenotrophomonas; antibiotic resistance; humans; multiple drug resistance; public health; reptiles; therapeutics; veterinary medicine; Iberian Peninsula
Abstract:
... Literature related to antimicrobial resistant (AMR) bacteria in exotic pets is minimal, being essential to report objective data on this topic, which represents a therapeutic challenge for veterinary medicine and public health. Between 2016 and 2020, laboratory records of 3156 exotic pet specimens’ microbiological diagnoses and antibiotic susceptibility testing (AST) results were examined. The sam ...
... AIMS: The objective of this study was to isolate multifunctional bacteriocin‐producing strains; to characterize the expressed bacteriocin for the control of Listeria monocytogenes and vancomycin‐resistant Enterococcus; to evaluate the safety of studied strains; and to explore their antifungal activity. METHODS AND RESULTS: Two Pediococcus strains were isolated from silage samples obtained from an ...
... Abuse and misuse of antimicrobial agents has accelerated the spread of antimicrobial-resistant bacteria. The association between antimicrobial-resistant infections in humans and antimicrobial use in agriculture is complex, but well-documented. This study provides a systematic review and meta-analysis of the dissemination of antimicrobial resistance (AMR) to antimicrobials defined as critically imp ...
... BACKGROUND: Surveillance of antimicrobial resistance (AMR) in horses is important to aid empirical treatment decisions and highlight emerging AMR threats. OBJECTIVE: To describe the AMR patterns of common groups of bacteria from clinical submissions from horses in the UK during 2018, and to determine how this varies by sample site and type of submitting veterinary practice. STUDY DESIGN: Prospecti ...
... New techniques are needed to speed-up the identification and antimicrobial susceptibility testing (AST) of bacteria associated with bloodstream infections. Alfred 60/AST (Alifax®, Polverara, Italy) performs AST by light scattering directly from positive blood cultures. We evaluated Alfred 60/AST performances for 4 months. Each new episode of bacteraemia was included and AST were compared to either ...
Enterococcus; ciprofloxacin; detection limit; disinfection; heterotrophs; irrigation; laws and regulations; municipal wastewater; oxidants; oxidation; pH; regrowth; temperature; total organic carbon; wastewater treatment
Abstract:
... To address the increasing contamination of aquatic environments and incidence of waterborne diseases, advanced oxidation processes with activated persulfate have emerged as tools to inactivate wastewater microorganisms and contaminants. In this work, the disinfection of a secondary effluent from a wastewater treatment plant by iron-based persulfate activation was studied. Experiments in a batch st ...
Enterococcus; Escherichia coli; European Union; empirical research; environmental education; intestines; public health; seawater; tourism; tourists; water quality; France; Italy; Mediterranean region; Spain
Abstract:
... Beach tourism in an important industry in many countries, and in the Mediterranean it supposes an extra pressure on the seawater quality. Concern about this aspect led the European Union to issue a first directive on the quality of bathing water in 1976 and modify it in 2006. This regulation establishes a mandatory control, in all European Union (EU) countries, of the proliferation of bacteria (sp ...
... Aspergillus-derived cellulase, which is involved in the production of Aspergillus-fermented foods, has been employed in the food and animal feed industries. However, the effect of dietary Aspergillus cellulase on health is poorly understood. Previously, we discovered that supplemental Aspergillus-derived protease and lipase preparations had substantial bifidogenic effects on rats fed a high-fat di ...
Amity G. Zimmer‐Faust; John F. Griffith; Joshua A. Steele; Laralyn Asato; Tania Chiem; Samuel Choi; Arturo Diaz; Joe Guzman; Michele Padilla; Jennipher Quach‐Cu; Victor Ruiz; Bryan Santos; Mary Woo; Stephen B. Weisberg
Enterococcus; United States Environmental Protection Agency; coliphages; health promotion; risk; water quality
Abstract:
... AIMS: Widespread adoption of the new U.S. Environmental Protection Agency (USEPA) Method 1642 for enumeration of coliphage in recreational water requires demonstration that laboratories consistently meet internal method performance goals and yield results that are consistent across laboratories. METHODS AND RESULTS: Here we assess the performance of six laboratories processing a series of blind wa ...
... More than 80% of wastewaters are discharged into rivers or seas, with a negative impact on water quality along the coast due to the presence of potential pathogens of faecal origin. Escherichia coli and enterococci are important indicators to assess, monitor, and predict microbial water quality in natural ecosystems. During rainfall events, the amount of wastewater delivered to rivers and coastal ...
... Microalgae are a source of bioactive compounds having recently been studied for their possible application as health-promoting ingredients. The aim of the study was to evaluate in an in vitro canine gut model the effects of four microalgae, Arthrospira platensis (AP), Haematococcus pluvialis (HP), Phaeodactylum tricornutum (PT) and Chlorella vulgaris (CV), on some fecal microbial populations and m ...
... Traditional hard Xinotyri cheese was manufactured using raw or pasteurized goat milk, without starter cultures, and the changes in microbiological and biochemical characteristics were studied during ripening and storage. Mesophilic lactic acid bacteria (LAB) predominated (>8.5 log CFU/g) in freshly fermented Xinotyri cheeses (pH 4.5–4.6), regardless of milk pasteurization. Enterobacteria, pseudomo ...
... Increasing reports have indicated that specific strains of probiotic Bacillus have the potential to prevent diseases. The purpose of this study was to explore the effects of three Bacillus strains (Bacillus subtilis BSWJ2017001, Bacillus pumilus BSWJ2017002, and B. subtilis BSWJ2017003) mixture dietary supplementation on rex rabbits infected with enterotoxigenic Escherichia coli (ETEC). In this st ...
... Inflammatory bowel disease could be induced by SARS-CoV-2, involved in alteration of gut microbiota during the respiratory viral infection. Presence of viral RNA in fecal samples for longer period, even after the clearance of the virus from respiratory tract, is suggestive of dysbiosis leading to the poor prognosis of COVID-19 in hospitalized patients. Gut microbiome (GM) plays a significant role ...
Acinetobacter; Enterococcus; Holstein; Jersey; Pseudomonas; Streptococcus uberis; aminoglycosides; beta-lactamase; cephalosporins; chloramphenicol; cross-sectional studies; dairy industry; lactation; milk; monsoon season; multiple drug resistance; public health; quinolones; udders; vancomycin; veterinary medicine
Abstract:
... Mastitis, which often manifests as udder infection in dairy animals, is of great concern as it affects public health and results in heavy economic losses to the dairy industry. A hospital-based cross-sectional study was conducted to determine the cultivable bacterial species associated with bovine clinical mastitis and their resistance patterns towards different antimicrobials. The milk samples fr ...
... BACKGROUND: SARS-CoV-2, a novel corona virus, has caused clusters of fatal pneumonia worldwide. Immune compromised patients are among the high risk groups with poor prognosis of the disease. The presence of bacterial or fungal co-infections with SARS-CoV-2 is associated with increased mortality. METHODS: The electronic data of the liver and kidney recipients, hospitalized in COVID-19 intensive car ...
... Coliphage are viruses that infect Escherichia coli (E. coli) and may indicate the presence of enteric viral pathogens in recreational waters. There is an increasing interest in using these viruses for water quality monitoring and forecasting; however, the ability to use statistical models to predict the concentrations of coliphage, as often done for cultured fecal indicator bacteria (FIB) such as ...
... The bacterial assemblages associated with Acropora palmata from the Mexican Caribbean Sea and the Gulf of Mexico were analyzed by high throughput sequencing of the 16S rRNA gene. Samples were taken from four substrates: apparently healthy corals, corals with white band disease, and the seawater and sediment surrounding these corals. The bacterial assemblages showed no significant differences among ...
... Accumulation of plastics in the marine environment has widespread detrimental consequences for ecosystems and wildlife. Marine plastics are rapidly colonised by a wide diversity of bacteria, including human pathogens, posing potential risks to health. Here, we investigate the effect of polymer type, residence time and estuarine location on bacterial colonisation of common household plastics, inclu ...
Burkholderia; Corynebacterium; Enterococcus; Flavobacterium; Pseudogymnoascus destructans; Pseudomonas; Rhinolophus ferrumequinum; bacterial communities; body condition; community structure; fungi; pathogens; temperature; white-nose syndrome
Abstract:
... The bats skin microbiota plays an important role in reducing pathogen infection, including the deadly fungal pathogen Pseudogymnoascus destructans, the causative agent of white‐nose syndrome. However, the dynamic of skin bacterial communities response to environmental perturbations remains poorly described. We characterized skin bacterial community over time and space in Rhinolophus ferrumequinum, ...
... The bacterial composition of naturally fermented Indian food, Idli was studied by high-throughput Illumina amplicon sequencing at different taxonomic levels. Metagenomic investigation revealed fold change with respect to some of the phylotypes in 06ᵗʰ to 12ᵗʰ h of fermentation, suggesting the synergistic mode of nutrition. After 12ᵗʰ h fermentation, bacterial populations were stabilized towards 15 ...
... A survey is presented concerning original research articles published in well-reputed scientific journals on the isolation of lactic acid bacteria (LAB) from cheeses worldwide, where researchers evaluated the bacteriocin production by such isolates in searching for novel functional peptides that can exhibit potential for biotechnological applications. Seventy-one articles were published in the per ...
Ileana Federigi; Elena Balestri; Alberto Castelli; Davide De Battisti; Ferruccio Maltagliati; Virginia Menicagli; Marco Verani; Claudio Lardicci; Annalaura Carducci
Enterococcus; One Health initiative; biomass; case studies; coastal zone management; data collection; databases; drainage; gastroenteritis; ingestion; marine debris; microbiological quality; models; pollution; risk; sand; sewage; shorelines; stakeholders; water currents; watersheds; Italy
Abstract:
... Beaches are affected by the accumulation of natural and anthropogenic material; however, this environmental issue has not yet been explored from a One Health perspective. In this paper, the conceptual framework of DPSIR (Drivers-Pressures-State-Impact-Response) was used to understand the beach-stranded material issue in a systemic way and a data-based classification for some environmental indicato ...
... To prevent foodborne diseases and extend shelf life, antimicrobial agents may be used in food to inhibit the growth of undesired microorganisms. The present study was aimed to determine the antimicrobial and antifungal activities of the fermented medicinal plants extract using Lactobacillus acidophilus ATCC 4356. The fermentation kinetic parameters, biochemical composition and the volatile compoun ...
... Bioaugmentation has many applications as a bioremediation technique. It is usually performed by bacteria but microalgal consortia also have great potential for bioremediation. This study evaluated the ability of a microbial consortium with predominance of microalgae (MCPM) to decontaminate the water of the Mallorquín tropical swamp. The Mallorquín Swamp is a natural water reservoir, essential for ...
Enterococcus; Rubus occidentalis; anthocyanins; arginine; biosynthesis; colon; dysbiosis; human health; humans; intestinal microorganisms; irritable bowel syndrome; liquid chromatography; lysine; mass spectrometry; metabolites; metabolomics; models; obesity; pH; proline; ribosomal DNA; temperature
Abstract:
... Human gut microbiota is critical for human health, as their dysbiosis could lead to various diseases such as irritable bowel syndrome and obesity. Black raspberry (BRB) has been increasingly studied recently for its impact on gut microbiota as a rich source of phytochemicals (e.g., anthocyanin). To investigate the effect of BRB extract on the gut microbiota composition and their metabolism, an in- ...
... Background: Bloodstream infections (BSI) caused by highly resistant pathogens in non-ICU COVID-19 departments pose important challenges. Methods: We performed a comparative analysis of incidence and microbial epidemiology of BSI in COVID-19 vs. non-COVID-19, non-ICU departments between 1 September 2020-31 October 2021. Risk factors for BSI and its impact on outcome were evaluated by a case-control ...
... Bombyx mori (BM) is an economically important insect for silk production, and it is also farmed and used as food in different countries around the world. The present systematic review aims to assess the suitability of BM as an edible insect, retrieving data from scientific papers reporting microbiological, chemical, and allergenic hazards of silkworm consumed as either whole insects or insect deri ...
... Camel milk is renowned for its nutritional value and its therapeutic properties. It is considered a promising alternative to bovine milk due to its higher nutritional benefits, hypoallergenic characteristics and greater digestibility in the human gastrointestinal system. This study reports camel milk's bacterial and fungal microbiota, and the effect of geographical location and season on its bacte ...
... Amaranth, quinoa, and buckwheat, known as pseudocereals, have been consumed since ancient times and are considered sacred in most cultures. Their grains can be used as cereals for breakfast or mixed with other grains in meals and their health-enhancing effects have been investigated more in recent years. They have an antioxidant effect and their nutrient profiles are enriched with processing techn ...
... In our study the presence of bacteria, yeast, and microscopic fungi was evaluated. Three forms of corn silage were made including silage without additive, silage with microbial additive (lactic acid bacteria), and silage with nutritional additive (urea). Silage additives were applied to the matter within the recommended dosage, then the matter was ensiled into plastic bags and stored at a constant ...
... This study explored the effects of glutelin digestion during rice grain storage on its biological activity and ability to modulate gut microbiota. In addition to structural changes, rice glutelin was investigated for its antioxidant activity, angiotensin-converting enzyme inhibition ability, properties of the peptides generated by its digestion, and effect on gut microbial composition. After 2 day ...
Mariya V. Gryaznova; Yuliya D. Dvoretskaya; Mikhail Y. Syromyatnikov; Sergey V. Shabunin; Pavel A. Parshin; Evgeniy V. Mikhaylov; Nikolay A. Strelnikov; Vasily N. Popov
... Determining the taxonomic composition of microbial consortia of the piglet intestine is of great importance for pig production. However, knowledge on the variety of the intestinal microbiome in newborn piglets is limited. Piglet diarrhea is a serious gastrointestinal disease with a high morbidity and mortality that causes great economic damage to the pig industry. In this study, we investigated th ...
Enterococcus; Hypsizygus marmoreus; Pediococcus; Pseudomonas; Serratia; Stenotrophomonas; spoilage; storage time
Abstract:
... The succession of bacterial communities and changes of volatile components throughout the storage of white Hypsizygus marmoreus (white H.marmoreus) were investigated. The results showed that the microbial communities were mainly composed of Proteobacteria and Firmicutes at 4 °C and 25 °C, with Serratia, Pediococcus, Enterococcus, Pseudomonas and Stenotrophomonas as the dominant genera. Serratia co ...
Enterobacteriaceae; Enterococcus; ammonia; cadaverine; dimethyl disulfide; food science; ham; histamine; humans; methanethiol; off odors; putrescine; salt content; spoilage; total volatile basic nitrogen
Abstract:
... To investigate the spoilage characteristics of Jinhua ham, sensory scores, volatile compounds, biogenic amine, physicochemical parameters and microbial counts were evaluated between normal and spoiled hams. The results showed that off-odors of spoiled hams were dominated by rancid, sour, sulfide and ammonia odors derived from these compounds including butanoic acid, methanethiol and dimethyl disul ...
Enterococcus; Lactobacillus; blue cheese; cows; dairy technology; goat milk; lactic acid; pH; pasteurization; pasteurized milk; protected designation of origin; proteolysis; raw milk; sensory evaluation
Abstract:
... Valdeón cheese is a Spanish Protected Geographical Indication of blue‐veined cheese produced on an industrial scale from raw or pasteurised cow and/or goat milk. The aim of this work was to evaluate the impact of pasteurisation on the microbiological, physicochemical and sensory characteristics of cheese. Cheeses made with raw milk showed higher counts of lactobacilli and enterococci, as well as l ...
... AIMS: To isolate and enrich lignocellulolytic microbial consortia from yak (Bos grunniens) rumen and evaluate their effects on the fermentation characteristics and enzymatic hydrolysis in rice straw silage. METHODS AND RESULTS: A novel microbial consortium M2 with high CMCase and xylanase activities was enriched and observed to be prone to use natural carbon sources. Its predominant genus was Ente ...
... Grasses exhibit variations in chemical composition and epiphytic microbiota during daylight. These variations may influence the natural fermentation parameters of silage. This study was to evaluate the bacterial community and fermentation parameters during ensiling of Napier grasses harvested at various times within a day. Secondary growth of Napier grass (vegetative stage) was harvested at 7:00 ( ...
Rodrigo J. N. Calumby; Lara M. de Almeida; Yasmin N. de Barros; Wilson D. Segura; Valcilaine T. Barbosa; Antonio T. da Silva; Camila B. Dornelas; Valter Alvino; Luciano A. M. Grillo
... Rhynchophorus palmarum Linnaeus is an agricultural pest that affects various palm crops, including coconut (Cocos nucifera) plantations which are prominent in the economy of Northeastern Brazil. Characterization of the intestinal microbiota of R. palmarum, as well as elucidation of aspects related to the biochemistry and physiology of the insect's digestion, is essential for intervention in specif ...
... This study aimed to investigate the changes in microbial diversity, and volatile compounds of traditional fermented soybean paste originated from Henan province of China using 16S rRNA sequencing, headspace solid phase microextraction-gas chromatography/mass spectrometry (HS-SPME-GC/MS), and electronic nose (E-nose). The amino acid nitrogen and reducing sugar contents ranged between 1.18 and 1.58 ...
Jumana Alja'fari; Sybil Sharvelle; Nichole E. Brinkman; Michael Jahne; Scott Keely; Emily A. Wheaton; Jay Garland; Claire Welty; Michael C. Sukop; Thomas Meixner
Campylobacter; Cryptosporidium parvum; Enterococcus; Escherichia coli; Giardia lamblia; Salmonella; chemical oxygen demand; citizen science; climate; data collection; droplets; genes; land use; microbiological quality; polymerase chain reaction; rain; research; runoff; storms; turbidity; water supply
Abstract:
... Roof runoff has the potential to serve as an important local water source in regions with growing populations and limited water supply. Given the scarcity of guidance regulating the use of roof runoff, a need exists to characterize the microbial quality of roof runoff. The objective of this 2-year research effort was to examine roof runoff microbial quality in four U.S. cities: Fort Collins, CO; T ...
Matteo Daghio; Francesco Pini; Anna Espinoza-Tofalos; Giuseppe Conte; Eleonora Mari; Fabiola Giannerini; Luciana Giovannetti; Arianna Buccioni; Andrea Franzetti; Lisa Granchi; Marcello Mele; Giulia Rampazzo; Teresa Gazzotti; Elisa Zironi; Carlo Viti
... Pink discoloration defect can cause economic losses for cheese producers due to the impossibility to sell the defected cheese, but few knowledge is currently available on the causes of this defect. To gain more insight on the causes that lead to the formation of pink discoloration in Pecorino Toscano cheese with the Protected Designation of Origin (PDO) status, the bacterial community in defected ...
Enterococcus; Lactobacillus; Lactococcus; casein; cheeses; free fatty acids; liquid chromatography; mass spectrometry; molecular weight; peptides; principal component analysis; protected designation of origin; proteolysis; relative humidity; temperature; volatile organic compounds
Abstract:
... A multiparameter study was performed to evaluate the effect of fondaco, a traditional ripening cellar without any artificial temperature and relative humidity control, on the chemical, microbiological, and sensory characteristics of Protected Geographical Indication Canestrato di Moliterno cheese. Ripening in such a nonconventional environment was associated with lower counts of lactococci, lactob ...
... Coccidiosis is a well-known poultry disease that causes the severe destruction of the intestinal tract, resulting in reduced growth performance and immunity, disrupted gut homeostasis and perturbed gut microbiota. Supplementation of probiotics were explored to play a key role in improving growth performance, enhancing innate and adaptive immunity, maintaining gut homeostasis and modulating gut mic ...
... Hyperuricemia is an independent hazard factor of renal injury and can induce renal fibrosis, promoting the development of chronic kidney disease (CKD). This study aimed to explore the probability of chlorogenic acid (CGA) as a potential substance for preventing hyperuricemia nephropathy (HN). Pretreatment with CGA downregulated SUA, BUN, and CR levels, relieved oxidative stress and inflammatory re ...
... In this study, we evaluated the antimicrobial susceptibility, the presence of gene-encoding virulence factors and CRISPR systems, as well as the ability to produce lytic enzymes among clinical E. faecalis and E. faecium isolates (n = 44). All enterococci isolates showed phenotypes of multidrug resistance. E. faecalis and E. faecium isolates exhibited high-level aminoglycoside resistance phenotype, ...
Luka Grbčić; Siniša Družeta; Goran Mauša; Tomislav Lipić; Darija Vukić Lušić; Marta Alvir; Ivana Lučin; Ante Sikirica; Davor Davidović; Vanja Travaš; Daniela Kalafatovic; Kristina Pikelj; Hana Fajković; Toni Holjević; Lado Kranjčević
Enterococcus; algorithms; coastal water; computer software; prediction; public health; salinity; water quality; Croatia
Abstract:
... Coastal water quality management is a public health concern, as water of poor quality can potentially harbor dangerous pathogens. In this study, we employ routine monitoring data of EscherichiaColi and enterococci across 15 beaches in the city of Rijeka, Croatia, to build machine learning models for predicting E.Coli and enterococci based on environmental features. Cross-validation analysis showed ...
... In this study, we applied a tube-based reverse transcription loop-mediated isothermal amplification technique using preloaded amplification and detection reagents for simple screening of viable vancomycin-resistant Enterococcus in a cordless manner. We adopted an mRNA-based approach to detect live Enterococcus in vancomycin-treated cultures. We used agarose to preload and store all reagents for am ...
... Antibiotics have been one of the most important discoveries in the area of applied medical microbiology; however, as a result of various factors, we are currently facing a dramatic and relatively dangerous increase in the number of cases of antibiotic resistance, and the need for new types of antimicrobials continues to grow. New approaches are needed to combat antibiotic-resistant pathogens. Bact ...
... Today, domestic cats are important human companion animals for their appearance and favorable personalities. During the history of their domestication, the morphological and genetic portraits of domestic cats changed significantly from their wild ancestors, and the gut microbial communities of different breeds of cats also apparently differ. In the current study, the gut microbiota of Ragdoll cats ...
... The gut microbiota is an integral part of the host and plays an important role in both growth and development of host. The research on intestinal microbiota of Nibea albiflora and its relationship to fish disease have not been reported before. This study aimed to investigate the composition and differences of gut bacteria between healthy and diseased Nibea albiflora. The intestines were collected ...
... Lactic acid bacteria (LAB) comprise a widespread bacterial group, inhabiting the niches of fermented vegetables and capable of producing beneficial organic acids. In the present study, several bioinformatics approaches were used to perform whole-genome sequencing and comparative genomics of five LAB species, Lactobacillus plantarum PC1–1, Pediococcus pentosaceus PC2–1(F2), Weissella hellenica PC1A ...
... We narratively reviewed the physiopathology, epidemiology, and management of co-infections in Clostridioides difficile colitis (CDI) by searching the following keywords in Embase, MedLine, and PubMed: “Clostridium/Clostridioides difficile”, “co-infection”, “blood-stream infection” (BSI), “fungemia”, “Candida”, “Cytomegalovirus”, “probiotics”, “microbial translocation” (MT). Bacterial BSIs (mainly ...
... Human gut microbiota is a protective agent of intestinal and systemic health, and its modulation is of great interest for human wellbeing. In the world of biotics, besides probiotics, prebiotics, and synbiotics, also appears the denomination of “postbiotics” and “psychobiotics”. Fermented dairy products are, traditionally, the major source of probiotics. Nevertheless, due to the increasing number ...
... In this study, copper oxide (CuO) catalyzed peroxydisulfate (PDS) system was investigated for the inactivation of a broad range of pathogenic microorganisms from urban wastewater. Complete inactivation of Escherichia coli, Enterococcus, F-specific RNA bacteriophages from secondary treated wastewater was achieved after a short time (15–30 min) treatment with CuO (10 g/L)/PDS (1 mM) system, but spor ...
... Stool is the most commonly used sample for gut microbiota analysis in humans and animals. Cryopreservation of stool at − 80 °C is a feasible and simple method in clinics and researches, especially in large-scale cohort studies. However, the viability of bacteria in stool after freezing has yet well-demonstrated quantitatively and compositionally. This study determined the viable microbiota of samp ...
... Integrated data from molecular and improved culturomics studies might offer holistic insights on gut microbiome dysbiosis triggered by xenobiotics, such as obesity and metabolic disorders. Bisphenol A (BPA), a dietary xenobiotic obesogen, was chosen for a directed culturing approach using microbiota specimens from 46 children with obesity and normal-weight profiles. In parallel, a complementary mo ...
František Zigo; Zuzana Farkašová; Jana Výrostková; Ivana Regecová; Silvia Ondrašovičová; Mária Vargová; Naďa Sasáková; Ewa Pecka-Kielb; Šárka Bursová; David Sandor Kiss
California mastitis test; DNA; Enterococcus; Escherichia coli; aminoglycosides; biofilm; cephalosporins; disk diffusion antimicrobial test; early lactation; hemolysis; macrolides; methicillin; milk; multiple drug resistance; resistance genes; udders; virulence; Czech Republic; Slovakia
Abstract:
... This study investigated 960 Slovak and Czech spotted cattle from four different conventional (non-organic) dairy herds located in Eastern Slovakia and Czechia during early lactation (14–100 days after calving). Dairy cows were examined clinically; milk from fore-stripping of each udder quarter was subjected to sensory examination and assessed by the California mastitis test (CMT), and laboratory a ...
... Newly hatched chicks do not have access to feed until 48-72 h post-hatch based on standard practices in the poultry industry. How these practices affect the chicken's immune system is not well understood. In this study, we investigated the effect of a 48-h delay in access to feed on the expression of specific immune-related genes in the gut (ileum) of newly hatched chicks. We also analyzed the cor ...
... BACKGROUND: Urinary tract infections (UTI) caused by Escherichia coli and Enterococcus spp., which are frequently coisolated in polymicrobial UTI, cause morbidity among dogs and warrant antimicrobial therapy. OBJECTIVES: To evaluate clinical features of dogs with polymicrobial E. coli and Enterococcal UTI. ANIMALS: Forty‐four client‐owned dogs with polymicrobial bacteriuria and groups of 100 clien ...
... Molecular genetic studies of stools were performed to assess the spread of some clinically relevant antimicrobial resistance determinants (ARD) in a gentoo penguin (Pygoscelis papua) and an Antarctic fur seal (Arctocephalus gazella) on Livingston Island. Glycopeptide resistance genes (vanA/vanD and vanB) were detected in both fecal samples, while the penguin's one was also mecA-positive and blaNDM ...
... Quantification of lactic acid bacteria (LAB) is essential to control quality of seafood products like cold-smoked salmon (CSS). In the present study, we report the design and optimization of a dual-labelled TaqMan ™ probe targeting the V7 region of 16S rRNA gene for the detection of LAB in CSS. This quantitative PCR (qPCR) assays is useful for the simultaneous detection of the ten LAB genera commu ...
... Larvae of the greater wax moth (Galleria mellonella) are an emerging animal model to study the innate immune response and biodegradation of plastic polymers. Both of these complex biological processes are likely impacted by the plasticity of host–microbe interactions, which remains understudied in lepidopterans. Consequently, we carried out 16S rRNA sequencing to explore the effect diet (natural, ...
... Previously, we have reported that the intake of oyster extract (OE), prepared from Pacific oysters (Crassostrea gigas), can attenuate symptoms of dextran sulfate sodium (DSS)-induced acute experimental colitis in mice. Herein, we aimed to evaluate whether OE intake ameliorates chronic experimental colitis induced by repeated DSS administration in mice. Male C57BL/6J (4-week-old) mice were fed eith ...
... Heavy metals (HMs) contaminated fish is a threat to humans when consumed. Dietary probiotics have evolved as a successful HMs removal approach. In this study, probiotics Enterococcus (EC) sp. and Lactococcus (LC) sp. were evaluated for toxicity alleviation and gut microbiota maintenance in Cyprinus carpio (single and combined approach) on Cr, Cd, and Cu mixture (0.8 mg/L and 1.6 mg/L) exposure (28 ...
... Copper and Zinc oxides nanoparticles (CuO and ZnO NPs, respectively) are among the most produced and commonly used engineered nanomaterials. They can be released into the environment, thereby causing health concerns and risks to biodiversity that indicate a need to evaluate their toxicological effects in a complex situation. Here, we used the insect model organism silkworm Bombyx mori to address t ...
... Larvae of superworms (Zophobas atratus Fab.) and yellow mealworms (Tenebrio molitor Linn.) can survive on sole plastic diets. However, no side-by-side comparison of plastics degradation by both species is available yet. Here, superworms and yellow mealworms were fed with polystyrene (PS) or polyurethane (PU) foam plastics as sole diets for 35 days with bran as control. Superworms survived 100% on ...
... The microbial diversity in Bactrian camel milk and naturally fermented camel milk with unique nutritional properties and medicinal value in Alxa, Inner Mongolia, has been little reported. The results of this study showed that the dominant bacterial phyla in Bactrian camel milk and naturally fermented camel milk were Firmicutes and Proteobacteria, and dominant fungal phyla were Ascomycota and Basid ...
... The aim of this study was to evaluate and compare the impact of genotype and storage conditions (temperature and time) on microbiological contamination and eggshell quality. There were four genotypes of laying hens used, Czech Golden Spotted (CGS), Greenleg Partridge (GP), White Leghorn (WL) and commercial hybrid (CH) hens were included. After collection, the eggs were divided equally into five gr ...
... The maturation of infant gut microbiota has lifelong implications on health, which has been proposed as the major events during the first year of life. However, little is known about their dynamic colonization and influencing elements among the first two-year infancy as well as into the adulthood. So based on the 16S rRNA sequencing data among 30 healthy breast-feeding mother-infant pairs with nor ...
... To investigate the general fermentation characteristics of doenjang-meju (a Korean traditional soybean brick), they were periodically collected from eight different manufacturers during all fermentation stages, and their microbial communities and metabolic compounds (metabolites and volatile compounds) were analyzed. Bacillus and/or lactic acid bacteria (LAB, mainly Enterococcus) and Aspergillus w ...
... The human urinary microbiome, also termed urobiome, has been overlooked due to the clinical dogma of sterile urine, as reported by routine culture. However, evolving sensitive tools such as expanded quantitative urine culture, 16S ribosomal RNA gene sequencing, and next-generation sequencing have discovered a vast number of microorganisms present in urine, even in healthy individuals. Microbiome d ...
... SCOPE: Many infants suffer from complementary feeding‐induced diarrhea (CFID). Studies have shown that intestinal microbes can enhance the intestinal barrier and prevent diarrhea by producing indole derivatives that promote pregnane X receptor (PXR) expression. METHODS AND RESULTS: In this study, the indole test and determination of the PXR concentration are performed on tryptophan broth cultures ...
... Intense rainfall can affect bathing water quality, especially in areas with poorly developed sewage systems or combined sewer overflows (CSOs). The aim of this study was to assess the impact of precipitation on coastal bathing water quality in the area of Split and Kaštela (Adriatic Sea), the urban areas where CSOs were applied. The study was conducted during two bathing seasons, 2020 and 2021. Th ...
... This study investigated the effects of WO₃-based photocatalytic bactericide on the piggery environment, microorganisms in pig manure, and weaned piglets. After spraying WO₃-based photocatalytic bactericide, the NH₃ concentration in the piggery decreased by 31.25% within 1 h and then returned to pre-spraying concentration after 24 h. The airborne aerobic bacteria level in the piggery before sprayin ...
... The effects of a blend of encapsulated organic acids with essential oils (EOA) as an alternative to antimicrobial growth promoter (AGP) on growth performance and gut health of Eimeria spp./Clostridium perfringens (C. perfringens) in chickens infected with necrotic enteritis (NE) broilers was investigated. A total of 432 male Arbor Acres broilers (1-day-old) were randomly distributed into 6 treatme ...
... One of the most extensively applied animal feed additives is yeast culture (YC), which can increase production efficiency by altering gastrointestinal tract (GIT) microbiota. Geese are the ideal model to study the interaction between diet and GIT, due to their adaptation to consume different roughage sources. Here, we fed geese with 0 (control), 0.5% (treat1), 1.0% (treat2), 2.0% (treat3), and 4.0 ...
... This study systematically evaluated and compared different inoculum pretreatment methods to quickly select dark fermentative bacteria from anaerobic sludge for the bioconversion of food waste. The hydrogen (H₂) production rate was found to be highest for 'heat + CO₂′ treated inoculum at 140.75 ± 2.61 mL/L/h compared to control experiments (60.27 ± 2.61 mL/L/h). At the same time, H₂ yield was found ...
Enterococcus; compliance; macroalgae; marine pollution; seawater; water quality; weather
Abstract:
... In this study, density plume visualizations and statistical comparisons were made of enterococci bacteria (the main marine recreational microbial water quality indicator) densities, both before and after the upgrade of the discharge from the South Bay Ocean Outfall (SBOO) to secondary treatment level, so that the effect of this upgrade on ocean microbial water quality could be assessed. During the ...
Enterococcus; chemical oxygen demand; coagulation; disinfection; flocculants; flocculation; most probable number technique; odors; oxidation; pH; plate count; polyacrylamide; potassium; proteolysis; research; response surface methodology; total nitrogen; total organic carbon; total phosphorus; wastewater; wastewater treatment
Abstract:
... Carwash wastewater treatment with potassium ferrate (VI) (K₂FeO₄) was optimized by response surface methodology. The optimum conditions for chemical oxygen demand removal were established a pH 3.5, 0.328 g/L dose of K₂FeO₄, and with a process duration of 48 min. At these conditions, chemical oxygen demand, total organic carbon, total nitrogen, and total phosphorus decreased by 70.3, 58.9, 73.3, 82 ...
... Primary influent and final effluent samples were collected from wastewater treatment plants using either chlorination or ultraviolet (UV) disinfection biweekly for one year. Paired measurements were determined for fecal indicator bacteria (Escherichia coli and enterococci), cultivated bacteriophages (somatic, F+, and CB-390 coliphage and GB-124 Bacteroides phage), human-associated viral markers (h ...
Enterococcus; Tenericutes; body temperature; body weight; cecum; dietary supplements; feed intake; heat stress; hydrolysis; livestock productivity; microorganisms; triacylglycerols; virulence; weight loss
Abstract:
... Heat stress (HS) negatively influences livestock productivity, but it can be, at least in part, mitigated by nutritional interventions. One such intervention is to use byproducts from various sources that are likely to be included in the consumer chain. Thus, the present study investigated the effects of dietary supplementation of solubles from shredded, steam-exploded pine particles (SSPPs) on th ...
... The intestinal disease coccidiosis, caused by parasitic Eimeria species, severely impacts poultry production, leading to an estimated $14 billion in annual losses worldwide. As the poultry industry moves away from antibiotics as a treatment for diseases, a better understanding of the microbiota is required to develop other solutions such as probiotics, prebiotics, and nutritional supplements. This ...
... Zearalenone (ZEN) is an estrogenic mycotoxin, and chickens are relatively insensitive to it. In this study, the effects of intestinal microorganisms on ZEN metabolism and toxicity mitigation in broilers were studied by two experiments. Firstly, in vitro, ZEN was incubated anaerobically with chyme from each part of the chicken intestine to study its intestinal microbial metabolism. Then, in vivo, w ...
... It is a valuable attempt to use Tartary buckwheat as the raw material of soybean paste due to its functional composition. In this research, the soybean pastes were prepared with Tartary buckwheat flour and wheat flour by low salt natural fermentation (10%, w/v NaCl), respectively; the physicochemical properties and microbial community structure of samples prepared from the two groups were investig ...