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... Enzymatically pre-treated sugarcane bagasse (SCB) was used as substrate for sequential production of hydrogen (H₂) and methane (CH₄). In stage I, SCB was used by autochthonous bacteria, such as Enterococcus (42.4 % relative abundance) and Paraclostridium (16.8 %) for H₂ production (166.8 mL H₂/L), while the metabolites accumulated at the end of this stage (9140.5 mg HAc/L) were used by allochthono ...
... The correlation of antioxidant activity and prebiotic effect about oat phenolic compounds was invested, while there exists limited studies. Free and bound phenolic compounds were separated from ethyl acetate, n-butanol and aqueous fractions. Fluorescence in situ hybridization was used to investigate gut microbiota of in vitro fermentation samples. The results showed that ethyl acetate fraction pos ...
... The gut microbiota consists a diverse and complex ecosystem that is involved in beneficial functions as well as potentially harmful conditions for human. Blastocystis sp. is a common parasite of the digestive tract of animals and humans; however, limited data is available concerning the association of asymptomatic Blastocystis infection and gut bacteria composition. Hence, in this cross-sectional ...
Actinobacteria; Enterococcus; Lactococcus; Paenibacillus; Proteobacteria; Sporosarcina; altitude; atmospheric pressure; biodegradation; biosynthesis; body temperature; cecum; chickens; cold; energy intake; genes; ileum; intestinal microorganisms; microbiome; oxygen; signal transduction; xenobiotics
Abstract:
... Low oxygen levels and extremely cold weather in high-altitude environments requires more energy intake to maintain body temperature in animals. However, little is known about the characteristics of cecal and ileac microbiota in Tibetan chicken and how the high and low altitude environments affect the gut microbiota communities in Tibetan chicken. In the present study, In the present study, Tibetan ...
... This study aimed to investigate the presence of eight virulence genes (ace, asa1, esp, efaA, gelE, cylA, agg, fsr) in Enterococcus from a variety of animals and to explore the drug resistance and pathogenicity. This could provide a theoretical basis for clinical treatment of Enterococcus infections. Anal swabs from pigs, chickens, cattle, and dogs in farms and pet hospitals were collected for Ente ...
... Knowing the antibiotic resistance profile of pathogenic bacteria is essential to finding an appropriate remedy for these issues by sourcing antibacterial agents from plants. The present study exposes the bactericidal potential of different leaf extracts of Blepharis maderaspatensis and Ziziphus oenoplia against multi drug resistant bacterial strains enumerated from pus specimens at a secondary car ...
... Abuse and misuse of antimicrobial agents has accelerated the spread of antimicrobial-resistant bacteria. The association between antimicrobial-resistant infections in humans and antimicrobial use in agriculture is complex, but well-documented. This study provides a systematic review and meta-analysis of the dissemination of antimicrobial resistance (AMR) to antimicrobials defined as critically imp ...
... New techniques are needed to speed-up the identification and antimicrobial susceptibility testing (AST) of bacteria associated with bloodstream infections. Alfred 60/AST (Alifax®, Polverara, Italy) performs AST by light scattering directly from positive blood cultures. We evaluated Alfred 60/AST performances for 4 months. Each new episode of bacteraemia was included and AST were compared to either ...
Enterococcus; Escherichia coli; European Union; empirical research; environmental education; intestines; public health; seawater; tourism; tourists; water quality; France; Italy; Mediterranean region; Spain
Abstract:
... Beach tourism in an important industry in many countries, and in the Mediterranean it supposes an extra pressure on the seawater quality. Concern about this aspect led the European Union to issue a first directive on the quality of bathing water in 1976 and modify it in 2006. This regulation establishes a mandatory control, in all European Union (EU) countries, of the proliferation of bacteria (sp ...
Amity G. Zimmer‐Faust; John F. Griffith; Joshua A. Steele; Laralyn Asato; Tania Chiem; Samuel Choi; Arturo Diaz; Joe Guzman; Michele Padilla; Jennipher Quach‐Cu; Victor Ruiz; Bryan Santos; Mary Woo; Stephen B. Weisberg
Enterococcus; United States Environmental Protection Agency; coliphages; health promotion; risk; water quality
Abstract:
... AIMS: Widespread adoption of the new U.S. Environmental Protection Agency (USEPA) Method 1642 for enumeration of coliphage in recreational water requires demonstration that laboratories consistently meet internal method performance goals and yield results that are consistent across laboratories. METHODS AND RESULTS: Here we assess the performance of six laboratories processing a series of blind wa ...
... Traditional hard Xinotyri cheese was manufactured using raw or pasteurized goat milk, without starter cultures, and the changes in microbiological and biochemical characteristics were studied during ripening and storage. Mesophilic lactic acid bacteria (LAB) predominated (>8.5 log CFU/g) in freshly fermented Xinotyri cheeses (pH 4.5–4.6), regardless of milk pasteurization. Enterobacteria, pseudomo ...
... Inflammatory bowel disease could be induced by SARS-CoV-2, involved in alteration of gut microbiota during the respiratory viral infection. Presence of viral RNA in fecal samples for longer period, even after the clearance of the virus from respiratory tract, is suggestive of dysbiosis leading to the poor prognosis of COVID-19 in hospitalized patients. Gut microbiome (GM) plays a significant role ...
Acinetobacter; Enterococcus; Holstein; Jersey; Pseudomonas; Streptococcus uberis; aminoglycosides; beta-lactamase; cephalosporins; chloramphenicol; cross-sectional studies; dairy industry; lactation; milk; monsoon season; multiple drug resistance; public health; quinolones; udders; vancomycin; veterinary medicine
Abstract:
... Mastitis, which often manifests as udder infection in dairy animals, is of great concern as it affects public health and results in heavy economic losses to the dairy industry. A hospital-based cross-sectional study was conducted to determine the cultivable bacterial species associated with bovine clinical mastitis and their resistance patterns towards different antimicrobials. The milk samples fr ...
... The bacterial assemblages associated with Acropora palmata from the Mexican Caribbean Sea and the Gulf of Mexico were analyzed by high throughput sequencing of the 16S rRNA gene. Samples were taken from four substrates: apparently healthy corals, corals with white band disease, and the seawater and sediment surrounding these corals. The bacterial assemblages showed no significant differences among ...
... Accumulation of plastics in the marine environment has widespread detrimental consequences for ecosystems and wildlife. Marine plastics are rapidly colonised by a wide diversity of bacteria, including human pathogens, posing potential risks to health. Here, we investigate the effect of polymer type, residence time and estuarine location on bacterial colonisation of common household plastics, inclu ...
Burkholderia; Corynebacterium; Enterococcus; Flavobacterium; Pseudogymnoascus destructans; Pseudomonas; Rhinolophus ferrumequinum; bacterial communities; body condition; community structure; fungi; pathogens; temperature; white-nose syndrome
Abstract:
... The bats skin microbiota plays an important role in reducing pathogen infection, including the deadly fungal pathogen Pseudogymnoascus destructans, the causative agent of white‐nose syndrome. However, the dynamic of skin bacterial communities response to environmental perturbations remains poorly described. We characterized skin bacterial community over time and space in Rhinolophus ferrumequinum, ...
... A survey is presented concerning original research articles published in well-reputed scientific journals on the isolation of lactic acid bacteria (LAB) from cheeses worldwide, where researchers evaluated the bacteriocin production by such isolates in searching for novel functional peptides that can exhibit potential for biotechnological applications. Seventy-one articles were published in the per ...
Ileana Federigi; Elena Balestri; Alberto Castelli; Davide De Battisti; Ferruccio Maltagliati; Virginia Menicagli; Marco Verani; Claudio Lardicci; Annalaura Carducci
Enterococcus; One Health initiative; biomass; case studies; coastal zone management; data collection; databases; drainage; gastroenteritis; ingestion; marine debris; microbiological quality; models; pollution; risk; sand; sewage; shorelines; stakeholders; water currents; watersheds; Italy
Abstract:
... Beaches are affected by the accumulation of natural and anthropogenic material; however, this environmental issue has not yet been explored from a One Health perspective. In this paper, the conceptual framework of DPSIR (Drivers-Pressures-State-Impact-Response) was used to understand the beach-stranded material issue in a systemic way and a data-based classification for some environmental indicato ...
... To prevent foodborne diseases and extend shelf life, antimicrobial agents may be used in food to inhibit the growth of undesired microorganisms. The present study was aimed to determine the antimicrobial and antifungal activities of the fermented medicinal plants extract using Lactobacillus acidophilus ATCC 4356. The fermentation kinetic parameters, biochemical composition and the volatile compoun ...
... Bioaugmentation has many applications as a bioremediation technique. It is usually performed by bacteria but microalgal consortia also have great potential for bioremediation. This study evaluated the ability of a microbial consortium with predominance of microalgae (MCPM) to decontaminate the water of the Mallorquín tropical swamp. The Mallorquín Swamp is a natural water reservoir, essential for ...
Enterococcus; Rubus occidentalis; anthocyanins; arginine; biosynthesis; colon; dysbiosis; human health; humans; intestinal microorganisms; irritable bowel syndrome; liquid chromatography; lysine; mass spectrometry; metabolites; metabolomics; models; obesity; pH; proline; ribosomal DNA; temperature
Abstract:
... Human gut microbiota is critical for human health, as their dysbiosis could lead to various diseases such as irritable bowel syndrome and obesity. Black raspberry (BRB) has been increasingly studied recently for its impact on gut microbiota as a rich source of phytochemicals (e.g., anthocyanin). To investigate the effect of BRB extract on the gut microbiota composition and their metabolism, an in- ...
... Bombyx mori (BM) is an economically important insect for silk production, and it is also farmed and used as food in different countries around the world. The present systematic review aims to assess the suitability of BM as an edible insect, retrieving data from scientific papers reporting microbiological, chemical, and allergenic hazards of silkworm consumed as either whole insects or insect deri ...
Enterococcus; Hypsizygus marmoreus; Pediococcus; Pseudomonas; Serratia; Stenotrophomonas; spoilage; storage time
Abstract:
... The succession of bacterial communities and changes of volatile components throughout the storage of white Hypsizygus marmoreus (white H.marmoreus) were investigated. The results showed that the microbial communities were mainly composed of Proteobacteria and Firmicutes at 4 °C and 25 °C, with Serratia, Pediococcus, Enterococcus, Pseudomonas and Stenotrophomonas as the dominant genera. Serratia co ...
Enterobacteriaceae; Enterococcus; ammonia; cadaverine; dimethyl disulfide; food science; ham; histamine; humans; methanethiol; off odors; putrescine; salt content; spoilage; total volatile basic nitrogen
Abstract:
... To investigate the spoilage characteristics of Jinhua ham, sensory scores, volatile compounds, biogenic amine, physicochemical parameters and microbial counts were evaluated between normal and spoiled hams. The results showed that off-odors of spoiled hams were dominated by rancid, sour, sulfide and ammonia odors derived from these compounds including butanoic acid, methanethiol and dimethyl disul ...
... AIMS: To isolate and enrich lignocellulolytic microbial consortia from yak (Bos grunniens) rumen and evaluate their effects on the fermentation characteristics and enzymatic hydrolysis in rice straw silage. METHODS AND RESULTS: A novel microbial consortium M2 with high CMCase and xylanase activities was enriched and observed to be prone to use natural carbon sources. Its predominant genus was Ente ...
... Grasses exhibit variations in chemical composition and epiphytic microbiota during daylight. These variations may influence the natural fermentation parameters of silage. This study was to evaluate the bacterial community and fermentation parameters during ensiling of Napier grasses harvested at various times within a day. Secondary growth of Napier grass (vegetative stage) was harvested at 7:00 ( ...
Jumana Alja'fari; Sybil Sharvelle; Nichole E. Brinkman; Michael Jahne; Scott Keely; Emily A. Wheaton; Jay Garland; Claire Welty; Michael C. Sukop; Thomas Meixner
Campylobacter; Cryptosporidium parvum; Enterococcus; Escherichia coli; Giardia lamblia; Salmonella; chemical oxygen demand; citizen science; climate; data collection; droplets; genes; land use; microbiological quality; polymerase chain reaction; rain; research; runoff; storms; turbidity; water supply
Abstract:
... Roof runoff has the potential to serve as an important local water source in regions with growing populations and limited water supply. Given the scarcity of guidance regulating the use of roof runoff, a need exists to characterize the microbial quality of roof runoff. The objective of this 2-year research effort was to examine roof runoff microbial quality in four U.S. cities: Fort Collins, CO; T ...
Enterococcus; Lactobacillus; Lactococcus; casein; cheeses; free fatty acids; liquid chromatography; mass spectrometry; molecular weight; peptides; principal component analysis; protected designation of origin; proteolysis; relative humidity; temperature; volatile organic compounds
Abstract:
... A multiparameter study was performed to evaluate the effect of fondaco, a traditional ripening cellar without any artificial temperature and relative humidity control, on the chemical, microbiological, and sensory characteristics of Protected Geographical Indication Canestrato di Moliterno cheese. Ripening in such a nonconventional environment was associated with lower counts of lactococci, lactob ...
... Coccidiosis is a well-known poultry disease that causes the severe destruction of the intestinal tract, resulting in reduced growth performance and immunity, disrupted gut homeostasis and perturbed gut microbiota. Supplementation of probiotics were explored to play a key role in improving growth performance, enhancing innate and adaptive immunity, maintaining gut homeostasis and modulating gut mic ...
... In this study, we evaluated the antimicrobial susceptibility, the presence of gene-encoding virulence factors and CRISPR systems, as well as the ability to produce lytic enzymes among clinical E. faecalis and E. faecium isolates (n = 44). All enterococci isolates showed phenotypes of multidrug resistance. E. faecalis and E. faecium isolates exhibited high-level aminoglycoside resistance phenotype, ...
Luka Grbčić; Siniša Družeta; Goran Mauša; Tomislav Lipić; Darija Vukić Lušić; Marta Alvir; Ivana Lučin; Ante Sikirica; Davor Davidović; Vanja Travaš; Daniela Kalafatovic; Kristina Pikelj; Hana Fajković; Toni Holjević; Lado Kranjčević
Enterococcus; algorithms; coastal water; computer software; prediction; public health; salinity; water quality; Croatia
Abstract:
... Coastal water quality management is a public health concern, as water of poor quality can potentially harbor dangerous pathogens. In this study, we employ routine monitoring data of EscherichiaColi and enterococci across 15 beaches in the city of Rijeka, Croatia, to build machine learning models for predicting E.Coli and enterococci based on environmental features. Cross-validation analysis showed ...
... In this study, we applied a tube-based reverse transcription loop-mediated isothermal amplification technique using preloaded amplification and detection reagents for simple screening of viable vancomycin-resistant Enterococcus in a cordless manner. We adopted an mRNA-based approach to detect live Enterococcus in vancomycin-treated cultures. We used agarose to preload and store all reagents for am ...
... Antibiotics have been one of the most important discoveries in the area of applied medical microbiology; however, as a result of various factors, we are currently facing a dramatic and relatively dangerous increase in the number of cases of antibiotic resistance, and the need for new types of antimicrobials continues to grow. New approaches are needed to combat antibiotic-resistant pathogens. Bact ...
... Today, domestic cats are important human companion animals for their appearance and favorable personalities. During the history of their domestication, the morphological and genetic portraits of domestic cats changed significantly from their wild ancestors, and the gut microbial communities of different breeds of cats also apparently differ. In the current study, the gut microbiota of Ragdoll cats ...
... Lactic acid bacteria (LAB) comprise a widespread bacterial group, inhabiting the niches of fermented vegetables and capable of producing beneficial organic acids. In the present study, several bioinformatics approaches were used to perform whole-genome sequencing and comparative genomics of five LAB species, Lactobacillus plantarum PC1–1, Pediococcus pentosaceus PC2–1(F2), Weissella hellenica PC1A ...
... Daqu, an indispensable starter, significantly affects the quality and yield of Chinese baijiu. To explore the bioturbation effect of space mutation and biofortification on strong-flavor Daqu, the microbial community, physicochemical properties and overall metabolic profile were investigated. Lactobacillus and Aspergillus were significantly reduced after bioturbation, while seven genera (e.g., Baci ...
... Human gut microbiota is a protective agent of intestinal and systemic health, and its modulation is of great interest for human wellbeing. In the world of biotics, besides probiotics, prebiotics, and synbiotics, also appears the denomination of “postbiotics” and “psychobiotics”. Fermented dairy products are, traditionally, the major source of probiotics. Nevertheless, due to the increasing number ...
... Newly hatched chicks do not have access to feed until 48-72 h post-hatch based on standard practices in the poultry industry. How these practices affect the chicken's immune system is not well understood. In this study, we investigated the effect of a 48-h delay in access to feed on the expression of specific immune-related genes in the gut (ileum) of newly hatched chicks. We also analyzed the cor ...
... BACKGROUND: Urinary tract infections (UTI) caused by Escherichia coli and Enterococcus spp., which are frequently coisolated in polymicrobial UTI, cause morbidity among dogs and warrant antimicrobial therapy. OBJECTIVES: To evaluate clinical features of dogs with polymicrobial E. coli and Enterococcal UTI. ANIMALS: Forty‐four client‐owned dogs with polymicrobial bacteriuria and groups of 100 clien ...
... Quantification of lactic acid bacteria (LAB) is essential to control quality of seafood products like cold-smoked salmon (CSS). In the present study, we report the design and optimization of a dual-labelled TaqMan ™ probe targeting the V7 region of 16S rRNA gene for the detection of LAB in CSS. This quantitative PCR (qPCR) assays is useful for the simultaneous detection of the ten LAB genera commu ...
... Larvae of the greater wax moth (Galleria mellonella) are an emerging animal model to study the innate immune response and biodegradation of plastic polymers. Both of these complex biological processes are likely impacted by the plasticity of host–microbe interactions, which remains understudied in lepidopterans. Consequently, we carried out 16S rRNA sequencing to explore the effect diet (natural, ...
... Previously, we have reported that the intake of oyster extract (OE), prepared from Pacific oysters (Crassostrea gigas), can attenuate symptoms of dextran sulfate sodium (DSS)-induced acute experimental colitis in mice. Herein, we aimed to evaluate whether OE intake ameliorates chronic experimental colitis induced by repeated DSS administration in mice. Male C57BL/6J (4-week-old) mice were fed eith ...
... Copper and Zinc oxides nanoparticles (CuO and ZnO NPs, respectively) are among the most produced and commonly used engineered nanomaterials. They can be released into the environment, thereby causing health concerns and risks to biodiversity that indicate a need to evaluate their toxicological effects in a complex situation. Here, we used the insect model organism silkworm Bombyx mori to address t ...
... The aim of this study was to evaluate and compare the impact of genotype and storage conditions (temperature and time) on microbiological contamination and eggshell quality. There were four genotypes of laying hens used, Czech Golden Spotted (CGS), Greenleg Partridge (GP), White Leghorn (WL) and commercial hybrid (CH) hens were included. After collection, the eggs were divided equally into five gr ...
... To investigate the general fermentation characteristics of doenjang-meju (a Korean traditional soybean brick), they were periodically collected from eight different manufacturers during all fermentation stages, and their microbial communities and metabolic compounds (metabolites and volatile compounds) were analyzed. Bacillus and/or lactic acid bacteria (LAB, mainly Enterococcus) and Aspergillus w ...
... The human urinary microbiome, also termed urobiome, has been overlooked due to the clinical dogma of sterile urine, as reported by routine culture. However, evolving sensitive tools such as expanded quantitative urine culture, 16S ribosomal RNA gene sequencing, and next-generation sequencing have discovered a vast number of microorganisms present in urine, even in healthy individuals. Microbiome d ...
... The effects of a blend of encapsulated organic acids with essential oils (EOA) as an alternative to antimicrobial growth promoter (AGP) on growth performance and gut health of Eimeria spp./Clostridium perfringens (C. perfringens) in chickens infected with necrotic enteritis (NE) broilers was investigated. A total of 432 male Arbor Acres broilers (1-day-old) were randomly distributed into 6 treatme ...
... This study systematically evaluated and compared different inoculum pretreatment methods to quickly select dark fermentative bacteria from anaerobic sludge for the bioconversion of food waste. The hydrogen (H₂) production rate was found to be highest for 'heat + CO₂′ treated inoculum at 140.75 ± 2.61 mL/L/h compared to control experiments (60.27 ± 2.61 mL/L/h). At the same time, H₂ yield was found ...
Enterococcus; chemical oxygen demand; coagulation; disinfection; flocculants; flocculation; most probable number technique; odors; oxidation; pH; plate count; polyacrylamide; potassium; proteolysis; research; response surface methodology; total nitrogen; total organic carbon; total phosphorus; wastewater; wastewater treatment
Abstract:
... Carwash wastewater treatment with potassium ferrate (VI) (K₂FeO₄) was optimized by response surface methodology. The optimum conditions for chemical oxygen demand removal were established a pH 3.5, 0.328 g/L dose of K₂FeO₄, and with a process duration of 48 min. At these conditions, chemical oxygen demand, total organic carbon, total nitrogen, and total phosphorus decreased by 70.3, 58.9, 73.3, 82 ...
... Primary influent and final effluent samples were collected from wastewater treatment plants using either chlorination or ultraviolet (UV) disinfection biweekly for one year. Paired measurements were determined for fecal indicator bacteria (Escherichia coli and enterococci), cultivated bacteriophages (somatic, F+, and CB-390 coliphage and GB-124 Bacteroides phage), human-associated viral markers (h ...