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Enterococcus; Escherichia coli; One Health initiative; aminoglycosides; animal health; antibiotic resistance; antibiotic resistance genes; game animals; humans; indicator species; livestock; metagenomics; microbiome; monitoring; omnivores; phenotype; pollution; wild boars; wildlife; Europe
Abstract:
... Antimicrobial resistance (AMR) is a serious problem for public and animal health, and also for the environment. Monitoring and reporting the occurrence of AMR determinants and bacteria with the potential to disseminate is a priority for health surveillance programs around the world and critical to the One Health concept. Wildlife is a reservoir of AMR, and human activities can strongly influence t ...
... The gut microbiota consists a diverse and complex ecosystem that is involved in beneficial functions as well as potentially harmful conditions for human. Blastocystis sp. is a common parasite of the digestive tract of animals and humans; however, limited data is available concerning the association of asymptomatic Blastocystis infection and gut bacteria composition. Hence, in this cross-sectional ...
... This work investigated the microbial community in Kantuan‐sliced chicken during retail storage at different temperatures (4 ± 1°C and 25 ± 1°C). Compared with the samples stored at 25°C, a lower storage temperature (4°C) could prolong the shelf life of Kantuan‐sliced chicken, as revealed by a lower change rate in pH value, total volatile basic nitrogen, and total viable counts. For the freshly pre ...
... Knowing the antibiotic resistance profile of pathogenic bacteria is essential to finding an appropriate remedy for these issues by sourcing antibacterial agents from plants. The present study exposes the bactericidal potential of different leaf extracts of Blepharis maderaspatensis and Ziziphus oenoplia against multi drug resistant bacterial strains enumerated from pus specimens at a secondary car ...
Acinetobacter; Enterococcus; Holstein; Jersey; Pseudomonas; Streptococcus uberis; aminoglycosides; beta-lactamase; cephalosporins; chloramphenicol; cross-sectional studies; dairy industry; lactation; milk; monsoon season; multiple drug resistance; public health; quinolones; udders; vancomycin; veterinary medicine
Abstract:
... Mastitis, which often manifests as udder infection in dairy animals, is of great concern as it affects public health and results in heavy economic losses to the dairy industry. A hospital-based cross-sectional study was conducted to determine the cultivable bacterial species associated with bovine clinical mastitis and their resistance patterns towards different antimicrobials. The milk samples fr ...
Burkholderia; Corynebacterium; Enterococcus; Flavobacterium; Pseudogymnoascus destructans; Pseudomonas; Rhinolophus ferrumequinum; bacterial communities; body condition; community structure; fungi; pathogens; temperature; white-nose syndrome
Abstract:
... The bats skin microbiota plays an important role in reducing pathogen infection, including the deadly fungal pathogen Pseudogymnoascus destructans, the causative agent of white‐nose syndrome. However, the dynamic of skin bacterial communities response to environmental perturbations remains poorly described. We characterized skin bacterial community over time and space in Rhinolophus ferrumequinum, ...
... Bioaugmentation has many applications as a bioremediation technique. It is usually performed by bacteria but microalgal consortia also have great potential for bioremediation. This study evaluated the ability of a microbial consortium with predominance of microalgae (MCPM) to decontaminate the water of the Mallorquín tropical swamp. The Mallorquín Swamp is a natural water reservoir, essential for ...
Enterococcus; Rubus occidentalis; anthocyanins; arginine; biosynthesis; colon; dysbiosis; human health; humans; intestinal microorganisms; irritable bowel syndrome; liquid chromatography; lysine; mass spectrometry; metabolites; metabolomics; models; obesity; pH; proline; ribosomal DNA; temperature
Abstract:
... Human gut microbiota is critical for human health, as their dysbiosis could lead to various diseases such as irritable bowel syndrome and obesity. Black raspberry (BRB) has been increasingly studied recently for its impact on gut microbiota as a rich source of phytochemicals (e.g., anthocyanin). To investigate the effect of BRB extract on the gut microbiota composition and their metabolism, an in- ...
Enterobacteriaceae; Enterococcus; ammonia; cadaverine; dimethyl disulfide; food science; ham; histamine; humans; methanethiol; off odors; putrescine; salt content; spoilage; total volatile basic nitrogen
Abstract:
... To investigate the spoilage characteristics of Jinhua ham, sensory scores, volatile compounds, biogenic amine, physicochemical parameters and microbial counts were evaluated between normal and spoiled hams. The results showed that off-odors of spoiled hams were dominated by rancid, sour, sulfide and ammonia odors derived from these compounds including butanoic acid, methanethiol and dimethyl disul ...
Enterococcus; Lactobacillus; Lactococcus; casein; cheeses; free fatty acids; liquid chromatography; mass spectrometry; molecular weight; peptides; principal component analysis; protected designation of origin; proteolysis; relative humidity; temperature; volatile organic compounds
Abstract:
... A multiparameter study was performed to evaluate the effect of fondaco, a traditional ripening cellar without any artificial temperature and relative humidity control, on the chemical, microbiological, and sensory characteristics of Protected Geographical Indication Canestrato di Moliterno cheese. Ripening in such a nonconventional environment was associated with lower counts of lactococci, lactob ...
... Human gut microbiota is a protective agent of intestinal and systemic health, and its modulation is of great interest for human wellbeing. In the world of biotics, besides probiotics, prebiotics, and synbiotics, also appears the denomination of “postbiotics” and “psychobiotics”. Fermented dairy products are, traditionally, the major source of probiotics. Nevertheless, due to the increasing number ...
... Newly hatched chicks do not have access to feed until 48-72 h post-hatch based on standard practices in the poultry industry. How these practices affect the chicken's immune system is not well understood. In this study, we investigated the effect of a 48-h delay in access to feed on the expression of specific immune-related genes in the gut (ileum) of newly hatched chicks. We also analyzed the cor ...
... Quantification of lactic acid bacteria (LAB) is essential to control quality of seafood products like cold-smoked salmon (CSS). In the present study, we report the design and optimization of a dual-labelled TaqMan ™ probe targeting the V7 region of 16S rRNA gene for the detection of LAB in CSS. This quantitative PCR (qPCR) assays is useful for the simultaneous detection of the ten LAB genera commu ...
... The aim of this study was to evaluate and compare the impact of genotype and storage conditions (temperature and time) on microbiological contamination and eggshell quality. There were four genotypes of laying hens used, Czech Golden Spotted (CGS), Greenleg Partridge (GP), White Leghorn (WL) and commercial hybrid (CH) hens were included. After collection, the eggs were divided equally into five gr ...
... To investigate the general fermentation characteristics of doenjang-meju (a Korean traditional soybean brick), they were periodically collected from eight different manufacturers during all fermentation stages, and their microbial communities and metabolic compounds (metabolites and volatile compounds) were analyzed. Bacillus and/or lactic acid bacteria (LAB, mainly Enterococcus) and Aspergillus w ...
... The human urinary microbiome, also termed urobiome, has been overlooked due to the clinical dogma of sterile urine, as reported by routine culture. However, evolving sensitive tools such as expanded quantitative urine culture, 16S ribosomal RNA gene sequencing, and next-generation sequencing have discovered a vast number of microorganisms present in urine, even in healthy individuals. Microbiome d ...
... The effects of a blend of encapsulated organic acids with essential oils (EOA) as an alternative to antimicrobial growth promoter (AGP) on growth performance and gut health of Eimeria spp./Clostridium perfringens (C. perfringens) in chickens infected with necrotic enteritis (NE) broilers was investigated. A total of 432 male Arbor Acres broilers (1-day-old) were randomly distributed into 6 treatme ...
... This study systematically evaluated and compared different inoculum pretreatment methods to quickly select dark fermentative bacteria from anaerobic sludge for the bioconversion of food waste. The hydrogen (H₂) production rate was found to be highest for 'heat + CO₂′ treated inoculum at 140.75 ± 2.61 mL/L/h compared to control experiments (60.27 ± 2.61 mL/L/h). At the same time, H₂ yield was found ...
... Primary influent and final effluent samples were collected from wastewater treatment plants using either chlorination or ultraviolet (UV) disinfection biweekly for one year. Paired measurements were determined for fecal indicator bacteria (Escherichia coli and enterococci), cultivated bacteriophages (somatic, F+, and CB-390 coliphage and GB-124 Bacteroides phage), human-associated viral markers (h ...
... BACKGROUND: Over the past years, antibiotic growth promoter had been restricted in animal husbandry production in many countries because of antimicrobial resistance and foodborne antibiotic residues. However, the problems of poor intestinal health and low growth efficiency of piglets have not been solved completely in an antibiotic-free diet, and it is urgent to explore alternatives to antimicrobi ...
... The aim of study was to determine the occurrence of virulence factors and virulence-related genes among enterococci isolated from food of animal origin and effects of osmotic and high pressure stress on expression of virulence-related genes. The number of 78 isolates were analyzed. None of them showed a strong ability to form biofilm, 38.5% (n = 30) had the slime production ability, 41% (n = 32) h ...
... Enterococci, which are on the WHO list of priority pathogens, are commonly encountered in hospital acquired infection and are becoming increasing significant due to the development of strains resistant to multiple antibiotics. Enterococci are also important microorganisms in the environment, and their presence is frequently used as an indicator of faecal pollution. Their success is related to thei ...
... Bacterial gut symbionts of insect herbivores can impact their host through different mechanisms. However, in most lepidopteran systems we lack experimental examples to explain how specific members of the gut bacterial community influence their host. We used fall armyworm (Spodoptera frugiperda) as a model system to address this objective. We implemented axenic and gnotobiotic techniques using two ...
... The production of short-chain fatty acids (SCFAs) via anaerobic fermentation of waste activated sludge (WAS) is often limited with poor quality of SCFAs and long fermentation time. To overcome these issues, we provided an efficient strategy by using ferric chloride (FC) to aid nitrite pretreatment. Experimental results showed that the maximal SCFAs production of 211.3 ± 3.1 mg COD/g VS was achieve ...
Bacteroides; Bifidobacterium; Eggerthella; Enterococcus; Ruminococcus; Streptococcus; Western diets; data collection; digestive system; feces; females; landscapes; livelihood; males; metagenomics; quality control; India
Abstract:
... The tribes of West Bengal are distributed in geographically distinct regions with distinctive features of their habitats and many of these tribes still practice a traditional livelihood avoiding the western diet. Hence, it is expected that their gut should remain pristine. In this study, we report the gut bacterial abundance of a Drukpa Bhutia tribal family of Lepchakha, inhabiting the hilly terra ...
... Inflammatory bowel disease is associated with intestinal homeostasis dysregulation and gut microbiota dysbiosis. This study aimed to investigate the protective effect of Ganoderma applanatum extracts (G. applanatum polysaccharides (GAP) and 75% ethanol extracts (GAE)) on colon inflammation and elucidate the therapeutic mechanism. GAP and GAE showed considerable protective effects against dextran s ...
... Chemical investigation of Australian pasture plant-derived Streptomyces sp. CMB-PB041, supported by miniaturized cultivation profiling and molecular network analysis, led to the isolation and characterization of 13 new macrocyclic spirotetronates, glenthmycins A–M (1–13), with structures assigned by detailed spectroscopic analysis, chemical degradation and derivatization, and mechanistic and biosy ...
I. Biasato; G. Chemello; S. Bellezza Oddon; I. Ferrocino; M.R. Corvaglia; C. Caimi; A. Resconi; A. Paul; M. van Spankeren; M.T. Capucchio; E. Colombino; L. Cocolin; F. Gai; A. Schiavone; L. Gasco
... The effects of including Hermetia illucens (HI) meal in rainbow trout diets have been widely characterized, but data related to its utilization in low-fishmeal (FM) diets are quite scarce. The current research investigated the impact of HI meal inclusion in low-FM diets for rainbow trout by assessing fish growth performance, nutrient digestibility, histomorphological traits of intestine and main o ...
Enterococcus; antibacterial properties; antibiotic resistance; bacterial infections; biocompatible materials; biofilm; drug development; heat; magnetism; methicillin-resistant Staphylococcus aureus; multiple drug resistance; nanocarriers; phagosomes; photons; plankton; public health
Abstract:
... The emergence and spread of antibiotic resistance is one of the biggest challenges in public health. There is an urgent need to discover novel agents against the occurrence of multidrug-resistant bacteria, such as methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci. The drug-resistant pathogens are able to grow and persist in infected sites, including biofilms, phagoso ...
... The purpose of this work was to reveal the relationship between the microbial diversity and flavor profiles of traditional dry-cured duck from a metabolomic perspective. Enterococcus, Psychrobacter, Macrococcus, Salinivbrio, and Staphylococcus were the dominant bacterial genera, while Trichophyton, Kurtzmaniella, Blumeria, Cladosporium, Lysurus, Aspergillus, Starmerella and Debaryomyces were the d ...
Joana C. Barbosa; Sónia Gonçalves; Marcin Makowski; Ítala C. Silva; Tânia Caetano; Tanja Schneider; Eva Mösker; Roderich D. Süssmuth; Nuno C. Santos; Sónia Mendo
Enterococcus; Gram-positive bacteria; antibiotic resistance; atomic force microscopy; lantibiotics; lipids; mechanism of action; methicillin-resistant Staphylococcus aureus; peptides; synergism; zeta potential
Abstract:
... Lantibiotics are promising candidates to address the worldwide problem of antibiotic resistance. They belong to a class of natural compounds exhibiting strong activity against clinically relevant Gram-positive bacterial strains, including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE). Lichenicidin is a class II two-peptide lantibiotic. The presence o ...
... Hermetia illucens larvae (black soldier fly larvae, BSFL) convert efficiently organic waste to high quality biomass. To gain knowledge on the specific functions of gut microbes in this process it is a prerequisite to culture members of the core gut microbiota. Two different cultivation strategies were applied here for this purpose, a dilution-to-extinction cultivation and direct plating using six ...
... Stacking is a widely used method of microbial enrichment in the field of fermentation and is traditionally used to promote flavor in Chinese sauce-flavor Baijiu; however, its precise mechanism is unknown. This study assessed the fermentation process of light-flavor Baijiu with the simplest microbial source. After comparing differences in the microbial composition of different kinds of Daqu, a high ...
... Shameta is a traditional, Ethiopian, cereal-based fermented porridge exclusively prepared for lactating mothers. The aim of this study was to determine the microbial quality of Shameta samples collected from households of lactating mothers and to determine microbial dynamics and physicochemical changes during laboratory fermentation of Shameta. Isolation and characterization of the dominant microb ...
... Several sources of water are used by farmers without concern for quality, which can have consequences on the health of the consumer of market garden products. The aim of this study is to evaluate the microbiological and physicochemical qualities of irrigation water. Microorganisms were counted using the membrane filtration and incorporation into the agar methods. The physicochemical parameters wer ...
Enterococcus; blood flow; epidemiological studies; females; geographical distribution; glycopeptides; longitudinal studies; males; molecular epidemiology; multilocus sequence typing; public health; Central European region; Germany
Abstract:
... Vancomycin-resistant enterococci (VRE) pose a public health challenge worldwide. While VRE bloodstream infections (VREBI) increase in Germany and Europe, population-based molecular data are scarce. We aimed to analyze the molecular epidemiology, demographic aspects, and geographical distribution of VREBI in the German Federal State of North-Rhine–Westphalia (NRW), located in the German–Dutch–Belgi ...
... BACKGROUND: The contribution of bacteria to fermented tea is not clear and the associated research is relatively limited. To reveal the role of microorganisms in fermented tea processing, the microbial community and metabolites of Fuzhuan brick tea (FBT), a Chinese traditional fermented tea, were revealed via high‐throughput sequencing and liquid chromatography‐mass spectrometry (LC‐MS). RESULTS: ...
... Umami peptides formed by microbial metabolism play an important role in the umami taste of Chouguiyu. In this study, 138 umami peptides and 6 kinds of proteases in two categories from 35 microbial genera were identified from Chouguiyu during the fermentation process by peptidomics and metagenomics analysis, respectively. The interaction network maps between the umami peptides and protease-producin ...
... Wastewater treatment plants (WWTPs) constantly receive a wide variety of contaminants, including pharmaceuticals, and are potential reservoirs of antibiotic resistance genes (ARGs). This favors the development of multidrug-resistant bacteria (MRB) through horizontal gene transfer. Samples from five different WWTP processes were collected in September 2020 and January 2021 to monitor ARG resistomes ...
Breno Bezerra Aragão; Sabrina Cândido Trajano; Raylson Pereira de Oliveira; Maria da Conceição Carvalho; Rafaella Grenfell de Carvalho; Maria Aparecida Juliano; José Wilton Pinheiro Junior; Rinaldo Aparecido Mota
Aerococcus viridans; Aureobasidium pullulans; Bacillus (bacteria); Corynebacterium; Enterobacter; Enterococcus; Filifactor villosus; Kocuria kristinae; Macrococcus caseolyticus; Staphylococcus; goat cheese; goats; human health; immunology; phenotype; polymerase chain reaction; traditional technology; Brazil
Abstract:
... Sanitary-hygienic failures in cheese making can pose health risks to consumers. This study aimed to identify multiresistant pathogens in different production stages of artisanal goat coalho cheese in Brazil and characterize their phenotypic and genotypic resistance. Eleven properties in the state of Pernambuco, Brazil, participated in the study. Samples were obtained from different stages of produ ...
Enterococcus; Escherichia coli; antibiotic resistance; hospitals; humans; organic matter; pollution; research; rivers; sediments; toxicity; wastewater; Democratic Republic of the Congo
Abstract:
... The co-occurrence of heavy metals, antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) from hospital effluents spreading into the river receiving systems and evaluating associated risks are topics of scientific interest and still under-studied in developing countries under tropical conditions. To understand the selectors of the ARGs, we examined the occurrence of heavy metal ...
... Community sewage is a useful medium for antimicrobial resistance (AMR) surveillance of populations residing in urban dwellings as it provides the resolution of tracking sources of AMR from different waste streams within a community. Antibiotic resistance profiles of 4 opportunistic pathogens were compared from 3 sewage sources: raw community sewage from 2 housing estates, a foreign workers dormito ...
Enterococcus; Escherichia coli; beta-lactamase; coliform bacteria; municipal wastewater; public health; quantitative polymerase chain reaction; risk; wastewater treatment; watersheds; Croatia
Abstract:
... Extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing Enterobacterales are a critical global health problem and wastewater treatment plants (WWTPs) can promote their spread into the environment; yet their efficacy is not well characterized. Here, we have used conventional culturing to monitor coliform bacteria and quantitative PCR to monitor 2 ESBL and 5 carbapenemase (CP) genes and 4 ...
... The unregulated probiotic market can lead to serious health consequences. The aim of this study was to analyze commercially available probiotics using high-throughput sequencing. Nine out of seventeen probiotics contained all the bacterial genera declared by the manufacturer, however, in addition to them, extraneous genera of bacteria were found. The most common genus of bacteria in the studied sa ...
Rajat Nag; Lauren Russell; Stephen Nolan; Agathe Auer; Bryan K. Markey; Paul Whyte; Vincent O'Flaherty; Declan Bolton; Owen Fenton; Karl G. Richards; Enda Cummins
... Farmyard manure and slurry (FYM&S) and anaerobic digestate are potentially valuable soil conditioners providing important nutrients for plant development and growth. However, these organic fertilisers may pose a microbial health risk to humans. A quantitative microbial risk assessment (QMRA) model was developed to investigate the potential human exposure to pathogens following the application of F ...
... The efficiency of microalgae harvesting on the removal of Giardia spp. cysts, Cryptosporidium spp. oocysts, total coliforms, Escherichia coli, Enterococcus spp. and Clostridium spp. was assessed in lab-scale experiments (Jartest and Flotatest) using effluent from a flat panel photobioreactor used for Chlorella sorokiniana cultivation. Three harvesting methods were evaluated: (1) flocculation induc ...
... The intestine is a potential location for berberine (BBR) to exert its therapeutic effects, but the understanding of the influences of BBR on the gut microbiota is limited. Through in vitro fermentation of human intestinal microbiota, we investigated the effects of BBR on microbiota composition and metabolism. The result indicated that BBR reduced the production of acetic acid and propionic acid a ...
... The influence of the inoculum on bacterial communities of Spanish-style green table olive brinesis very limited. This work assessed the size and structure of the bacterial communities in olive brines inoculated with Lactiplantibacillus plantarum. Also, a new qPCR assay was developed to determine the specific abundance of this genus. Absolute abundances of total Bacteria and L.plantarum decrease st ...
... Lipolytic enzymes are an important group of hydrolases that have found immense industrial application in biotechnology. In this study, the ability of gut bacteria isolated from the gut of the Eri silkworm, Samia ricini, to produce lipolytic enzymes was evaluated through qualitative and quantitative assays. The results of lipase screening showed that 28 isolates had lipolytic activity. The results ...
... Two exopolysaccharides (EPS) producing strains, isolated from raw donkey milk were identified as Leuconostoc mesenteroides SL and Enterococcus viikkiensis N5 using 16S rDNA sequencing. The Box Benheken design exhibited the highest yield of EPS-SL (672.342 mg/L) produced by SL and of EPS-N5 (901 mg/L) produced by N5. The molecular weight was 1.68×10⁴ for EPS-SL and 1.55×10⁴ Da for EPS-N5. FTIR, NMR ...
... Solar disinfection (SODIS) is an inexpensive drinking water treatment method applied in tropical and sub-tropical low-income countries. However, it has been unclear whether it functions adequately also in colder climates. To investigate this issue, SODIS experiments were performed in the humid continental climate of Finland by exposing faecally contaminated drinking water to natural solar radiatio ...
... Microorganisms, their activity, and metabolites are now considered as intrinsic elements of the human body and this awareness gave was leading to the concept of holobiont [...] ...
... This study was conducted to assess the impact of dietary incorporation of Spirulina platensis and selenium nanoparticles (SeNPs) individually or in combinations on growth performance, antioxidant status, humoral immune response, and microbial populations in diet and ileum of heat-stressed broilers. Ross-308 one-day chicks (n = 450) were fed one of 9 experimental diets with five replicate cages in ...
... Sulfite solution has been commonly used on trimmed young coconut (TYC) and other produce to prolong shelf life, but it has been banned in many countries due to its allergenic reaction in sensitive individuals. This study has developed sulfite-free treatment together with modified atmosphere packaging (MAP) which could prolong shelf life of TYC during cold storage. The main goal of this work was to ...
Enterobacteriaceae; Enterococcus; Leuconostoc carnosum; Leuconostoc gelidum; bacterial contamination; detection limit; heat treatment; lactic acid; plate count; raw materials; raw meat
Abstract:
... The aim of this study was to analyse the influence of the contamination level of fresh meat on the bacterial population in raw material before cooking and on the microbiota of cooked hams following heat treatment. The effect of incubation temperatures of 6.5 and 15 °C on the results obtained was also evaluated during the bacteriological investigation. The total viable count (TVC), the number of En ...
... Little is known about the genomic diversity of the microbial communities associated with raw municipal wastewater (sewage), including whether microbial populations specific to sewage exist and how such populations could be used to improve source attribution and apportioning in contaminated waters. Herein, we used the influent of three wastewater treatment plants in Atlanta, Georgia (USA) to pertur ...
... The aim of the present study was to obtain information on the occurrence of bacteria and eumycetes in ready-to-eat fermented liver sausages manufactured by 20 artisan producers located in the Marche Region (Italy). To this end, culture-dependent analyses and metataxonomic sequencing were carried out. Physico-chemical parameters and volatilome of the fermented liver sausages were also studied. Fina ...
Eleonora Riccobono; Tommaso Giani; Giulia Baldi; Sophie Arcangeli; Alberto Antonelli; Valeria Tellone; Alessandra Del Vecchio; Alessandra Capezzone De Joannon; Gian Maria Rossolini
Enterococcus; Russia; antibacterial properties; dalbavancin; methicillin-resistant Staphylococcus aureus; minimum inhibitory concentration; therapeutics; vancomycin; Eastern European region
Abstract:
... Gram-positive pathogens remain a major cause of healthcare- and community-associated infections. In particular, the dissemination of methicillin-resistant staphylococci, such as methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE), have significantly reduced the therapeutic options, making the management of these infections even more challenging. Dalbavanci ...
DNA; Enterococcus; Escherichia coli; United States Environmental Protection Agency; public health; quality control; research; rivers; water quality
Abstract:
... Rapid quantitative PCR-based methods for enterococci monitoring can allow public health authorities to make more timely beach posting decisions. However, qPCR methods must be assessed for proposed sites as locale-specific factors may affect DNA recovery or qPCR inhibition. We assessed the feasibility of the USEPA 1609.1 qPCR-based (Enterococcus) method at two urban Toronto beaches and three recrea ...
... The present study was carried to correlate the microbial diversity of the biofilms developed on the wooden boards used for aging traditional Sicilian cheeses with cheese typology. To this end, the microbial diversity of the shelves in contact with the cheeses PDO Pecorino Siciliano, PDO Piacentinu Ennese, and TAP Caciocavallo Palermitano, during ripening, was evaluated by a multivariate statistica ...
... Probiotic intake has been shown to improve certain physiological health indicators. We aimed to examine effects of Lactobacillus casei LTL1879, obtained from long-lived elderly volunteers, on blood biochemical, oxidative, and inflammatory markers and gut microbiota in twenty healthy, young volunteers. Volunteers were randomly divided into equal probiotic and placebo groups and changes in blood bio ...
... This study aimed to determine the bacterial isolates associated with postpartum endometritis among dairy cows in Western Australia and their antimicrobial susceptibility profiles. A cross‐sectional study was conducted between June–October 2020. Endometritis was defined as evidence of mucopurulent to purulent vaginal discharge 60–100 days postpartum. Vaginal discharge samples were obtained, culture ...
... Enzyme activity plays an important role in the functioning of aquatic ecosystems. It is sensitive to changes in environmental conditions such as pH, temperature, and nutrient concentration. The objective of this work was to determine the acid phosphatase activity (AcPA) in the Almendares and San Juan rivers (western Cuba) and its relationship with physicochemical and microbiological indicators. Fo ...
... The microbiome in gastrointestinal tracts play an important role in regulating nutrient utilization and absorption, gut immune function, and host growth or development. This study was conducted to investigate the composition and dynamic distribution of caecal microbiota in pullets during the first 16 weeks. Growth performance, immune organs index, and intestinal morphology of pullets were analyzed ...
... The role of the air as a vehicle of bacteria dissemination in the farming environment has been previously reported, but still scarcely studied. This study investigated the bacteria density/diversity of the inside and outside air and of litter samples at a broiler farm. Samples were collected considering two seasons, three outside air distances (50/100/150 m) and the four cardinal directions. Selec ...
... BACKGROUND: Rabbit coccidiosis is a major disease caused by various Eimeria species and causes enormous economic losses to the rabbit industry. Coccidia infection has a wide impact on the gut microbiota and intestinal biochemical equilibrium. In the present study, we established a model of Eimeria intestinalis infection in rabbits to evaluate the jejunal microbiota and fecal metabolite profiles. M ...
... AIMS: Beach water quality is regulated by faecal indicator bacteria levels, sand is not, despite known human health risk from exposure to beach sand. We compared the performance of three methods to extract bacterial DNA from beach sand as a step toward a standard method. METHODS AND RESULTS: The analytical sensitivity of quantitative polymerase chain reaction (qPCR) for Enterococcus was compared f ...
... The researches studied opportunistic microbiocenoses at different technological sites of a commercial dairy farm in a three-year dynamics. The composition and sensitivity of the opportunistic pathogenic microflora to antibiotics were determined. It was established that the generic and species composition of the core of microbiocenoses was represented by typical opportunistic strains and was charac ...
... BACKGROUND: Rotavirus (RV) is a principal cause of diarrhea. However, there is a limited understanding regarding alteration of the gut microbial community structure and abundance during RV infection. This study was to characterize any potential associations between RV infection and the intestinal microbiota. METHODS: Suckling mice were divided into normal group (NC) and infected group (RV) randoml ...
... Antimicrobials are widely applied in aquaculture for treatment of infectious diseases in fish. The increased antimicrobial resistance of fish pathogens to conventional antimicrobial treatment highlights the need for research on the antibacterial properties of natural products—in this case, essential oils (EOs). The aim of the present study was to detect the antimicrobial activity of the essential ...
... AIMS: The objective of this study was to isolate multifunctional bacteriocin‐producing strains; to characterize the expressed bacteriocin for the control of Listeria monocytogenes and vancomycin‐resistant Enterococcus; to evaluate the safety of studied strains; and to explore their antifungal activity. METHODS AND RESULTS: Two Pediococcus strains were isolated from silage samples obtained from an ...
... BACKGROUND: Surveillance of antimicrobial resistance (AMR) in horses is important to aid empirical treatment decisions and highlight emerging AMR threats. OBJECTIVE: To describe the AMR patterns of common groups of bacteria from clinical submissions from horses in the UK during 2018, and to determine how this varies by sample site and type of submitting veterinary practice. STUDY DESIGN: Prospecti ...
Enterococcus; ciprofloxacin; detection limit; disinfection; heterotrophs; irrigation; laws and regulations; municipal wastewater; oxidants; oxidation; pH; regrowth; temperature; total organic carbon; wastewater treatment
Abstract:
... To address the increasing contamination of aquatic environments and incidence of waterborne diseases, advanced oxidation processes with activated persulfate have emerged as tools to inactivate wastewater microorganisms and contaminants. In this work, the disinfection of a secondary effluent from a wastewater treatment plant by iron-based persulfate activation was studied. Experiments in a batch st ...
... Aspergillus-derived cellulase, which is involved in the production of Aspergillus-fermented foods, has been employed in the food and animal feed industries. However, the effect of dietary Aspergillus cellulase on health is poorly understood. Previously, we discovered that supplemental Aspergillus-derived protease and lipase preparations had substantial bifidogenic effects on rats fed a high-fat di ...
... The production of bio-hydrogen and bio-electricity from biological processes has become a relevant scientific subject in recent years, given that renewable energy can be obtained from organic substrate. Dark fermentation (DF), and microbial electrolysis cells (MECs) have been used to produce bio-hydrogen. In this study, a novel integrated single-stage DF-Microbial fuel cells (MFCs) system was deve ...
... Increasing reports have indicated that specific strains of probiotic Bacillus have the potential to prevent diseases. The purpose of this study was to explore the effects of three Bacillus strains (Bacillus subtilis BSWJ2017001, Bacillus pumilus BSWJ2017002, and B. subtilis BSWJ2017003) mixture dietary supplementation on rex rabbits infected with enterotoxigenic Escherichia coli (ETEC). In this st ...
... Azadirachtin is a good growth inhibitor for Lepidopteran larvae, but its effect on the brain neurons, intestinal flora and intestinal contents caused by the growth inhibition mechanism has not been reported yet. This study explored the mechanism of azadirachtin on the growth and development of Spodoptera litura larvae and brain neurons through three aspects: intestinal pathology observation, intes ...
... BACKGROUND: SARS-CoV-2, a novel corona virus, has caused clusters of fatal pneumonia worldwide. Immune compromised patients are among the high risk groups with poor prognosis of the disease. The presence of bacterial or fungal co-infections with SARS-CoV-2 is associated with increased mortality. METHODS: The electronic data of the liver and kidney recipients, hospitalized in COVID-19 intensive car ...
... Benzoic acid, the simplest member of the aromatic carboxylic acid family, is a weak acid that is a precursor to the synthesis of many important organic compounds. In addition to being used to prevent microbial spoilage in foods, it can also form due to different factors in foods. Hippuric acid found in the natural content of milk can be converted into benzoic acid naturally and can be found in dif ...
... To prevent foodborne diseases and extend shelf life, antimicrobial agents may be used in food to inhibit the growth of undesired microorganisms. The present study was aimed to determine the antimicrobial and antifungal activities of the fermented medicinal plants extract using Lactobacillus acidophilus ATCC 4356. The fermentation kinetic parameters, biochemical composition and the volatile compoun ...
Enterococcus; adsorption; biodegradation; bioremediation; carbon; color; culture media; energy; foams; high performance liquid chromatography; nitrites; nitrogen; pH; polyurethanes; research; stoichiometry; toxicity; wastewater; Red Sea; Saudi Arabia
Abstract:
... Nitroaromatic effluents are common wastes generated from different industrial activities. These wastes can cause various environmental problems due to their toxicity on living organisms and difficulties to decompose either chemically or biologically. However, picric acid (2,4,6-trinitrophenol, TNP) could be used as a sole carbon, nitrogen, and energy source by a few bacterial cells. In this study, ...
... Amaranth, quinoa, and buckwheat, known as pseudocereals, have been consumed since ancient times and are considered sacred in most cultures. Their grains can be used as cereals for breakfast or mixed with other grains in meals and their health-enhancing effects have been investigated more in recent years. They have an antioxidant effect and their nutrient profiles are enriched with processing techn ...
... This study investigated the taxonomic profile and abundance distribution of the bacterial community in the ceca of feral and pasture-raised broiler (commercial) chickens. Cecal content from feral and commercial chickens (n = 7 each) was collected, and total DNA was isolated. Next-Generation Sequencing (Illumina MiSeq) was performed to characterize the cecal microbiota. Specific bacteria explored w ...
... The eri silkworm, Samia ricini, is associated with symbiotic bacteria believed to be playing several physiological functions to the host. The larvae of S. ricini were subjected to isolation of gut bacteria using culture-dependent 16S rRNA gene amplification and sequencing; and qualitative and quantitative enzymatic assays. The results of qualitative cellulolytic assays indicated that 58% of the sc ...
... This study explored the effects of glutelin digestion during rice grain storage on its biological activity and ability to modulate gut microbiota. In addition to structural changes, rice glutelin was investigated for its antioxidant activity, angiotensin-converting enzyme inhibition ability, properties of the peptides generated by its digestion, and effect on gut microbial composition. After 2 day ...
Mariya V. Gryaznova; Yuliya D. Dvoretskaya; Mikhail Y. Syromyatnikov; Sergey V. Shabunin; Pavel A. Parshin; Evgeniy V. Mikhaylov; Nikolay A. Strelnikov; Vasily N. Popov
... Determining the taxonomic composition of microbial consortia of the piglet intestine is of great importance for pig production. However, knowledge on the variety of the intestinal microbiome in newborn piglets is limited. Piglet diarrhea is a serious gastrointestinal disease with a high morbidity and mortality that causes great economic damage to the pig industry. In this study, we investigated th ...
... The region around the plant root referred to as the rhizosphere, is the zone where various microbial activity occurs. It performs crucial functions such as increasing the uptake of nutrients for plant development and preventing plant against plant pathogens. Keeping in mind the beneficial role performed by rhizospheric microorganisms, rhizobacterial species were isolated from the maize and soybean ...
... Bacterial endophthalmitis is an intraocular infection that causes rapid vison loss. Pathogens can infect the intraocular space directly (exogenous endophthalmitis (ExE)) or indirectly (endogenous endophthalmitis (EnE)). To identify predictive factors for the visual prognosis of Japanese patients with bacterial endophthalmitis, we retrospectively examined the bacterial endophthalmitis characteristi ...
... Enterococci are opportunistic members of intestinal microbiota with notable ability to transmit antimicrobial resistance genes. Among the different resistance mechanisms, multidrug efflux is evolving as a huge problem in conferring multidrug resistance to bacterial cells because these pumps extrude a broad range of antimicrobials. Therefore, the aim of this work was to evaluate role of efflux pump ...
... The gut microbiota is an integral part of the host and plays an important role in both growth and development of host. The research on intestinal microbiota of Nibea albiflora and its relationship to fish disease have not been reported before. This study aimed to investigate the composition and differences of gut bacteria between healthy and diseased Nibea albiflora. The intestines were collected ...
... This study was ascertained to investigate the adverse effects of pathogenic E. coli on gut microbiota of Tibetan piglets with history of yellow and white dysentery. For this purpose, a total of 18 fecal samples were collected from infected and healthy Tibetan piglets for 16S rRNA gene amplification and sequencing of V3–V4 region. Results showed that Firmicutes, Bacteroidia Fusobacteriota, Proteoba ...
... Our objective was to analyze the diversity of the microbiota over 180 d of ripening of eight batches of artisanal goatskin Tulum cheeses by culture-dependent and culture-independent (PCR-DGGE) methods. V3 region of the bacterial 16S rRNA gene was amplified with the PCR after direct DNA isolation from the cheese samples. Nine different species and five genera were determined by culturing, while 11 ...
... In this study, we compared the microbiota and volatile organic compounds (VOC) present in the milk obtained from 3 different sheep breeds, namely Merino, Lacaune, and Assaf. Udder milk was collected from 21 animals, 7 from each breed. Bacterial microflora was determined metagenomically by extracting the DNA from the milk and analyzing the V3-V4 region of the 16S rRNA gene. Headspace solid-phase mi ...
... In order to mitigate the food animal sector’s role in the growing threat of antimicrobial resistance (AMR), the World Health Organization (WHO) suggests the use of lower tier antimicrobials, such as florfenicol. Florfenicol has two dosing schemes used to treat primarily bovine respiratory disease. In this study, the objective was to characterize the plasma and gastrointestinal pharmacokinetics of ...
... BACKGROUND: The Biological Isolation and Containment Unit (BICU) is a subunit of the Teaching Hospital of the Faculty of Veterinary Medicine of the University of Lisbon, Portugal, for the admission of animals with confirmed infectious diseases or under clinical suspicion and waiting for a diagnosis. As a high-risk environment for the transmission of infectious agents, it is extremely important to ...
... Chironomids are aquatic insects that are known to be pollution tolerant. We have recently demonstrated that endogenous chironomid microbiota protects its host from toxic metals. Following these findings, we hypothesized that under different environmental conditions, a different bacterial consortium will evolve. Our aim was to explore the change in chironomid larval microbiota composition triggered ...
... In this study, copper oxide (CuO) catalyzed peroxydisulfate (PDS) system was investigated for the inactivation of a broad range of pathogenic microorganisms from urban wastewater. Complete inactivation of Escherichia coli, Enterococcus, F-specific RNA bacteriophages from secondary treated wastewater was achieved after a short time (15–30 min) treatment with CuO (10 g/L)/PDS (1 mM) system, but spor ...
... Present research work was conducted to isolate lactic acid bacteria from the intestine of freshwater fish Cyprinus carpio. Total 35 isolates of this bacteria were isolated. Among these isolate N12 was studied for morphological, cultural, biochemical characters. It was tested for its ability to ferment 18 different sugars and their derivatives. Genotypic identification of the bacteria was carried o ...
... Integrated data from molecular and improved culturomics studies might offer holistic insights on gut microbiome dysbiosis triggered by xenobiotics, such as obesity and metabolic disorders. Bisphenol A (BPA), a dietary xenobiotic obesogen, was chosen for a directed culturing approach using microbiota specimens from 46 children with obesity and normal-weight profiles. In parallel, a complementary mo ...
... Molecular genetic studies of stools were performed to assess the spread of some clinically relevant antimicrobial resistance determinants (ARD) in a gentoo penguin (Pygoscelis papua) and an Antarctic fur seal (Arctocephalus gazella) on Livingston Island. Glycopeptide resistance genes (vanA/vanD and vanB) were detected in both fecal samples, while the penguin's one was also mecA-positive and blaNDM ...