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Enterococcus; Escherichia coli; One Health initiative; aminoglycosides; animal health; antibiotic resistance; antibiotic resistance genes; game animals; humans; indicator species; livestock; metagenomics; microbiome; monitoring; omnivores; phenotype; pollution; wild boars; wildlife; Europe
Abstract:
... Antimicrobial resistance (AMR) is a serious problem for public and animal health, and also for the environment. Monitoring and reporting the occurrence of AMR determinants and bacteria with the potential to disseminate is a priority for health surveillance programs around the world and critical to the One Health concept. Wildlife is a reservoir of AMR, and human activities can strongly influence t ...
... The gut microbiota consists a diverse and complex ecosystem that is involved in beneficial functions as well as potentially harmful conditions for human. Blastocystis sp. is a common parasite of the digestive tract of animals and humans; however, limited data is available concerning the association of asymptomatic Blastocystis infection and gut bacteria composition. Hence, in this cross-sectional ...
... This work investigated the microbial community in Kantuan‐sliced chicken during retail storage at different temperatures (4 ± 1°C and 25 ± 1°C). Compared with the samples stored at 25°C, a lower storage temperature (4°C) could prolong the shelf life of Kantuan‐sliced chicken, as revealed by a lower change rate in pH value, total volatile basic nitrogen, and total viable counts. For the freshly pre ...
... Knowing the antibiotic resistance profile of pathogenic bacteria is essential to finding an appropriate remedy for these issues by sourcing antibacterial agents from plants. The present study exposes the bactericidal potential of different leaf extracts of Blepharis maderaspatensis and Ziziphus oenoplia against multi drug resistant bacterial strains enumerated from pus specimens at a secondary car ...
Acinetobacter; Enterococcus; Holstein; Jersey; Pseudomonas; Streptococcus uberis; aminoglycosides; beta-lactamase; cephalosporins; chloramphenicol; cross-sectional studies; dairy industry; lactation; milk; monsoon season; multiple drug resistance; public health; quinolones; udders; vancomycin; veterinary medicine
Abstract:
... Mastitis, which often manifests as udder infection in dairy animals, is of great concern as it affects public health and results in heavy economic losses to the dairy industry. A hospital-based cross-sectional study was conducted to determine the cultivable bacterial species associated with bovine clinical mastitis and their resistance patterns towards different antimicrobials. The milk samples fr ...
Burkholderia; Corynebacterium; Enterococcus; Flavobacterium; Pseudogymnoascus destructans; Pseudomonas; Rhinolophus ferrumequinum; bacterial communities; body condition; community structure; fungi; pathogens; temperature; white-nose syndrome
Abstract:
... The bats skin microbiota plays an important role in reducing pathogen infection, including the deadly fungal pathogen Pseudogymnoascus destructans, the causative agent of white‐nose syndrome. However, the dynamic of skin bacterial communities response to environmental perturbations remains poorly described. We characterized skin bacterial community over time and space in Rhinolophus ferrumequinum, ...
... Bioaugmentation has many applications as a bioremediation technique. It is usually performed by bacteria but microalgal consortia also have great potential for bioremediation. This study evaluated the ability of a microbial consortium with predominance of microalgae (MCPM) to decontaminate the water of the Mallorquín tropical swamp. The Mallorquín Swamp is a natural water reservoir, essential for ...
Enterococcus; Rubus occidentalis; anthocyanins; arginine; biosynthesis; colon; dysbiosis; human health; humans; intestinal microorganisms; irritable bowel syndrome; liquid chromatography; lysine; mass spectrometry; metabolites; metabolomics; models; obesity; pH; proline; ribosomal DNA; temperature
Abstract:
... Human gut microbiota is critical for human health, as their dysbiosis could lead to various diseases such as irritable bowel syndrome and obesity. Black raspberry (BRB) has been increasingly studied recently for its impact on gut microbiota as a rich source of phytochemicals (e.g., anthocyanin). To investigate the effect of BRB extract on the gut microbiota composition and their metabolism, an in- ...
Enterobacteriaceae; Enterococcus; ammonia; cadaverine; dimethyl disulfide; food science; ham; histamine; humans; methanethiol; off odors; putrescine; salt content; spoilage; total volatile basic nitrogen
Abstract:
... To investigate the spoilage characteristics of Jinhua ham, sensory scores, volatile compounds, biogenic amine, physicochemical parameters and microbial counts were evaluated between normal and spoiled hams. The results showed that off-odors of spoiled hams were dominated by rancid, sour, sulfide and ammonia odors derived from these compounds including butanoic acid, methanethiol and dimethyl disul ...
Enterococcus; Lactobacillus; Lactococcus; casein; cheeses; free fatty acids; liquid chromatography; mass spectrometry; molecular weight; peptides; principal component analysis; protected designation of origin; proteolysis; relative humidity; temperature; volatile organic compounds
Abstract:
... A multiparameter study was performed to evaluate the effect of fondaco, a traditional ripening cellar without any artificial temperature and relative humidity control, on the chemical, microbiological, and sensory characteristics of Protected Geographical Indication Canestrato di Moliterno cheese. Ripening in such a nonconventional environment was associated with lower counts of lactococci, lactob ...
... Human gut microbiota is a protective agent of intestinal and systemic health, and its modulation is of great interest for human wellbeing. In the world of biotics, besides probiotics, prebiotics, and synbiotics, also appears the denomination of “postbiotics” and “psychobiotics”. Fermented dairy products are, traditionally, the major source of probiotics. Nevertheless, due to the increasing number ...
... Newly hatched chicks do not have access to feed until 48-72 h post-hatch based on standard practices in the poultry industry. How these practices affect the chicken's immune system is not well understood. In this study, we investigated the effect of a 48-h delay in access to feed on the expression of specific immune-related genes in the gut (ileum) of newly hatched chicks. We also analyzed the cor ...
... Quantification of lactic acid bacteria (LAB) is essential to control quality of seafood products like cold-smoked salmon (CSS). In the present study, we report the design and optimization of a dual-labelled TaqMan ™ probe targeting the V7 region of 16S rRNA gene for the detection of LAB in CSS. This quantitative PCR (qPCR) assays is useful for the simultaneous detection of the ten LAB genera commu ...
... The aim of this study was to evaluate and compare the impact of genotype and storage conditions (temperature and time) on microbiological contamination and eggshell quality. There were four genotypes of laying hens used, Czech Golden Spotted (CGS), Greenleg Partridge (GP), White Leghorn (WL) and commercial hybrid (CH) hens were included. After collection, the eggs were divided equally into five gr ...
... To investigate the general fermentation characteristics of doenjang-meju (a Korean traditional soybean brick), they were periodically collected from eight different manufacturers during all fermentation stages, and their microbial communities and metabolic compounds (metabolites and volatile compounds) were analyzed. Bacillus and/or lactic acid bacteria (LAB, mainly Enterococcus) and Aspergillus w ...
... The human urinary microbiome, also termed urobiome, has been overlooked due to the clinical dogma of sterile urine, as reported by routine culture. However, evolving sensitive tools such as expanded quantitative urine culture, 16S ribosomal RNA gene sequencing, and next-generation sequencing have discovered a vast number of microorganisms present in urine, even in healthy individuals. Microbiome d ...
... The effects of a blend of encapsulated organic acids with essential oils (EOA) as an alternative to antimicrobial growth promoter (AGP) on growth performance and gut health of Eimeria spp./Clostridium perfringens (C. perfringens) in chickens infected with necrotic enteritis (NE) broilers was investigated. A total of 432 male Arbor Acres broilers (1-day-old) were randomly distributed into 6 treatme ...
... This study systematically evaluated and compared different inoculum pretreatment methods to quickly select dark fermentative bacteria from anaerobic sludge for the bioconversion of food waste. The hydrogen (H₂) production rate was found to be highest for 'heat + CO₂′ treated inoculum at 140.75 ± 2.61 mL/L/h compared to control experiments (60.27 ± 2.61 mL/L/h). At the same time, H₂ yield was found ...
... Primary influent and final effluent samples were collected from wastewater treatment plants using either chlorination or ultraviolet (UV) disinfection biweekly for one year. Paired measurements were determined for fecal indicator bacteria (Escherichia coli and enterococci), cultivated bacteriophages (somatic, F+, and CB-390 coliphage and GB-124 Bacteroides phage), human-associated viral markers (h ...
... BACKGROUND: Over the past years, antibiotic growth promoter had been restricted in animal husbandry production in many countries because of antimicrobial resistance and foodborne antibiotic residues. However, the problems of poor intestinal health and low growth efficiency of piglets have not been solved completely in an antibiotic-free diet, and it is urgent to explore alternatives to antimicrobi ...
... The aim of study was to determine the occurrence of virulence factors and virulence-related genes among enterococci isolated from food of animal origin and effects of osmotic and high pressure stress on expression of virulence-related genes. The number of 78 isolates were analyzed. None of them showed a strong ability to form biofilm, 38.5% (n = 30) had the slime production ability, 41% (n = 32) h ...
... Enterococci, which are on the WHO list of priority pathogens, are commonly encountered in hospital acquired infection and are becoming increasing significant due to the development of strains resistant to multiple antibiotics. Enterococci are also important microorganisms in the environment, and their presence is frequently used as an indicator of faecal pollution. Their success is related to thei ...
... Bacterial gut symbionts of insect herbivores can impact their host through different mechanisms. However, in most lepidopteran systems we lack experimental examples to explain how specific members of the gut bacterial community influence their host. We used fall armyworm (Spodoptera frugiperda) as a model system to address this objective. We implemented axenic and gnotobiotic techniques using two ...
... The production of short-chain fatty acids (SCFAs) via anaerobic fermentation of waste activated sludge (WAS) is often limited with poor quality of SCFAs and long fermentation time. To overcome these issues, we provided an efficient strategy by using ferric chloride (FC) to aid nitrite pretreatment. Experimental results showed that the maximal SCFAs production of 211.3 ± 3.1 mg COD/g VS was achieve ...
Bacteroides; Bifidobacterium; Eggerthella; Enterococcus; Ruminococcus; Streptococcus; Western diets; data collection; digestive system; feces; females; landscapes; livelihood; males; metagenomics; quality control; India
Abstract:
... The tribes of West Bengal are distributed in geographically distinct regions with distinctive features of their habitats and many of these tribes still practice a traditional livelihood avoiding the western diet. Hence, it is expected that their gut should remain pristine. In this study, we report the gut bacterial abundance of a Drukpa Bhutia tribal family of Lepchakha, inhabiting the hilly terra ...
... Inflammatory bowel disease is associated with intestinal homeostasis dysregulation and gut microbiota dysbiosis. This study aimed to investigate the protective effect of Ganoderma applanatum extracts (G. applanatum polysaccharides (GAP) and 75% ethanol extracts (GAE)) on colon inflammation and elucidate the therapeutic mechanism. GAP and GAE showed considerable protective effects against dextran s ...
... Chemical investigation of Australian pasture plant-derived Streptomyces sp. CMB-PB041, supported by miniaturized cultivation profiling and molecular network analysis, led to the isolation and characterization of 13 new macrocyclic spirotetronates, glenthmycins A–M (1–13), with structures assigned by detailed spectroscopic analysis, chemical degradation and derivatization, and mechanistic and biosy ...
I. Biasato; G. Chemello; S. Bellezza Oddon; I. Ferrocino; M.R. Corvaglia; C. Caimi; A. Resconi; A. Paul; M. van Spankeren; M.T. Capucchio; E. Colombino; L. Cocolin; F. Gai; A. Schiavone; L. Gasco
... The effects of including Hermetia illucens (HI) meal in rainbow trout diets have been widely characterized, but data related to its utilization in low-fishmeal (FM) diets are quite scarce. The current research investigated the impact of HI meal inclusion in low-FM diets for rainbow trout by assessing fish growth performance, nutrient digestibility, histomorphological traits of intestine and main o ...
Enterococcus; antibacterial properties; antibiotic resistance; bacterial infections; biocompatible materials; biofilm; drug development; heat; magnetism; methicillin-resistant Staphylococcus aureus; multiple drug resistance; nanocarriers; phagosomes; photons; plankton; public health
Abstract:
... The emergence and spread of antibiotic resistance is one of the biggest challenges in public health. There is an urgent need to discover novel agents against the occurrence of multidrug-resistant bacteria, such as methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci. The drug-resistant pathogens are able to grow and persist in infected sites, including biofilms, phagoso ...
... The purpose of this work was to reveal the relationship between the microbial diversity and flavor profiles of traditional dry-cured duck from a metabolomic perspective. Enterococcus, Psychrobacter, Macrococcus, Salinivbrio, and Staphylococcus were the dominant bacterial genera, while Trichophyton, Kurtzmaniella, Blumeria, Cladosporium, Lysurus, Aspergillus, Starmerella and Debaryomyces were the d ...
Joana C. Barbosa; Sónia Gonçalves; Marcin Makowski; Ítala C. Silva; Tânia Caetano; Tanja Schneider; Eva Mösker; Roderich D. Süssmuth; Nuno C. Santos; Sónia Mendo
Enterococcus; Gram-positive bacteria; antibiotic resistance; atomic force microscopy; lantibiotics; lipids; mechanism of action; methicillin-resistant Staphylococcus aureus; peptides; synergism; zeta potential
Abstract:
... Lantibiotics are promising candidates to address the worldwide problem of antibiotic resistance. They belong to a class of natural compounds exhibiting strong activity against clinically relevant Gram-positive bacterial strains, including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE). Lichenicidin is a class II two-peptide lantibiotic. The presence o ...
... Hermetia illucens larvae (black soldier fly larvae, BSFL) convert efficiently organic waste to high quality biomass. To gain knowledge on the specific functions of gut microbes in this process it is a prerequisite to culture members of the core gut microbiota. Two different cultivation strategies were applied here for this purpose, a dilution-to-extinction cultivation and direct plating using six ...
... Stacking is a widely used method of microbial enrichment in the field of fermentation and is traditionally used to promote flavor in Chinese sauce-flavor Baijiu; however, its precise mechanism is unknown. This study assessed the fermentation process of light-flavor Baijiu with the simplest microbial source. After comparing differences in the microbial composition of different kinds of Daqu, a high ...
... Shameta is a traditional, Ethiopian, cereal-based fermented porridge exclusively prepared for lactating mothers. The aim of this study was to determine the microbial quality of Shameta samples collected from households of lactating mothers and to determine microbial dynamics and physicochemical changes during laboratory fermentation of Shameta. Isolation and characterization of the dominant microb ...
... Several sources of water are used by farmers without concern for quality, which can have consequences on the health of the consumer of market garden products. The aim of this study is to evaluate the microbiological and physicochemical qualities of irrigation water. Microorganisms were counted using the membrane filtration and incorporation into the agar methods. The physicochemical parameters wer ...
Enterococcus; blood flow; epidemiological studies; females; geographical distribution; glycopeptides; longitudinal studies; males; molecular epidemiology; multilocus sequence typing; public health; Central European region; Germany
Abstract:
... Vancomycin-resistant enterococci (VRE) pose a public health challenge worldwide. While VRE bloodstream infections (VREBI) increase in Germany and Europe, population-based molecular data are scarce. We aimed to analyze the molecular epidemiology, demographic aspects, and geographical distribution of VREBI in the German Federal State of North-Rhine–Westphalia (NRW), located in the German–Dutch–Belgi ...
... BACKGROUND: The contribution of bacteria to fermented tea is not clear and the associated research is relatively limited. To reveal the role of microorganisms in fermented tea processing, the microbial community and metabolites of Fuzhuan brick tea (FBT), a Chinese traditional fermented tea, were revealed via high‐throughput sequencing and liquid chromatography‐mass spectrometry (LC‐MS). RESULTS: ...
... Umami peptides formed by microbial metabolism play an important role in the umami taste of Chouguiyu. In this study, 138 umami peptides and 6 kinds of proteases in two categories from 35 microbial genera were identified from Chouguiyu during the fermentation process by peptidomics and metagenomics analysis, respectively. The interaction network maps between the umami peptides and protease-producin ...
... Wastewater treatment plants (WWTPs) constantly receive a wide variety of contaminants, including pharmaceuticals, and are potential reservoirs of antibiotic resistance genes (ARGs). This favors the development of multidrug-resistant bacteria (MRB) through horizontal gene transfer. Samples from five different WWTP processes were collected in September 2020 and January 2021 to monitor ARG resistomes ...
Breno Bezerra Aragão; Sabrina Cândido Trajano; Raylson Pereira de Oliveira; Maria da Conceição Carvalho; Rafaella Grenfell de Carvalho; Maria Aparecida Juliano; José Wilton Pinheiro Junior; Rinaldo Aparecido Mota
Aerococcus viridans; Aureobasidium pullulans; Bacillus (bacteria); Corynebacterium; Enterobacter; Enterococcus; Filifactor villosus; Kocuria kristinae; Macrococcus caseolyticus; Staphylococcus; goat cheese; goats; human health; immunology; phenotype; polymerase chain reaction; traditional technology; Brazil
Abstract:
... Sanitary-hygienic failures in cheese making can pose health risks to consumers. This study aimed to identify multiresistant pathogens in different production stages of artisanal goat coalho cheese in Brazil and characterize their phenotypic and genotypic resistance. Eleven properties in the state of Pernambuco, Brazil, participated in the study. Samples were obtained from different stages of produ ...
Enterococcus; Escherichia coli; antibiotic resistance; hospitals; humans; organic matter; pollution; research; rivers; sediments; toxicity; wastewater; Democratic Republic of the Congo
Abstract:
... The co-occurrence of heavy metals, antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) from hospital effluents spreading into the river receiving systems and evaluating associated risks are topics of scientific interest and still under-studied in developing countries under tropical conditions. To understand the selectors of the ARGs, we examined the occurrence of heavy metal ...
... Community sewage is a useful medium for antimicrobial resistance (AMR) surveillance of populations residing in urban dwellings as it provides the resolution of tracking sources of AMR from different waste streams within a community. Antibiotic resistance profiles of 4 opportunistic pathogens were compared from 3 sewage sources: raw community sewage from 2 housing estates, a foreign workers dormito ...
Enterococcus; Escherichia coli; beta-lactamase; coliform bacteria; municipal wastewater; public health; quantitative polymerase chain reaction; risk; wastewater treatment; watersheds; Croatia
Abstract:
... Extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing Enterobacterales are a critical global health problem and wastewater treatment plants (WWTPs) can promote their spread into the environment; yet their efficacy is not well characterized. Here, we have used conventional culturing to monitor coliform bacteria and quantitative PCR to monitor 2 ESBL and 5 carbapenemase (CP) genes and 4 ...
... The unregulated probiotic market can lead to serious health consequences. The aim of this study was to analyze commercially available probiotics using high-throughput sequencing. Nine out of seventeen probiotics contained all the bacterial genera declared by the manufacturer, however, in addition to them, extraneous genera of bacteria were found. The most common genus of bacteria in the studied sa ...
Rajat Nag; Lauren Russell; Stephen Nolan; Agathe Auer; Bryan K. Markey; Paul Whyte; Vincent O'Flaherty; Declan Bolton; Owen Fenton; Karl G. Richards; Enda Cummins
... Farmyard manure and slurry (FYM&S) and anaerobic digestate are potentially valuable soil conditioners providing important nutrients for plant development and growth. However, these organic fertilisers may pose a microbial health risk to humans. A quantitative microbial risk assessment (QMRA) model was developed to investigate the potential human exposure to pathogens following the application of F ...
... The efficiency of microalgae harvesting on the removal of Giardia spp. cysts, Cryptosporidium spp. oocysts, total coliforms, Escherichia coli, Enterococcus spp. and Clostridium spp. was assessed in lab-scale experiments (Jartest and Flotatest) using effluent from a flat panel photobioreactor used for Chlorella sorokiniana cultivation. Three harvesting methods were evaluated: (1) flocculation induc ...
... This study explored the effects of H₂O₂ on Cyanobacteria and non-target microbes using fluorometry, microscopy, flow cytometry, and high throughput DNA sequencing of the 16S rRNA gene during a series of mesocosm and whole-ecosystem experiments in a eutrophic pond in NY, USA. The addition of H₂O₂ (8 mg/L) significantly reduced Cyanobacteria concentrations during a majority of experiments (66%; 6 of ...
... The intestine is a potential location for berberine (BBR) to exert its therapeutic effects, but the understanding of the influences of BBR on the gut microbiota is limited. Through in vitro fermentation of human intestinal microbiota, we investigated the effects of BBR on microbiota composition and metabolism. The result indicated that BBR reduced the production of acetic acid and propionic acid a ...
... The influence of the inoculum on bacterial communities of Spanish-style green table olive brinesis very limited. This work assessed the size and structure of the bacterial communities in olive brines inoculated with Lactiplantibacillus plantarum. Also, a new qPCR assay was developed to determine the specific abundance of this genus. Absolute abundances of total Bacteria and L.plantarum decrease st ...
... Lipolytic enzymes are an important group of hydrolases that have found immense industrial application in biotechnology. In this study, the ability of gut bacteria isolated from the gut of the Eri silkworm, Samia ricini, to produce lipolytic enzymes was evaluated through qualitative and quantitative assays. The results of lipase screening showed that 28 isolates had lipolytic activity. The results ...