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... N-Heterocyclic carbene (NHC) is regarded as a versatile ligand to stabilize gold nanoclusters (AuNCs) via forming strong Au-C bonds. However, reports on NHC-protected AuNCs are rare, especially in aqueous systems. Herein, we present a facile one-step approach to fabricate hydrophilic AuNCs in an aqueous medium using a NHC-based ligand as both a reducing agent and stabilizer. The coordination mecha ...
Escherichia coli; Gram-negative bacteria; dyes; fluorescence recovery after photobleaching; lipopolysaccharides; membrane fluidity; models; polymyxin B; temperature
Abstract:
... The outer membrane (OM) is an essential component of the Gram‐negative bacterial cell envelope. Restricted diffusion of integral OM proteins and lipopolysaccharide (LPS) that constitute the outer leaflet of the OM support a model in which the OM is in a semi‐crystalline state. The low fluidity of the OM has been suggested to be an important property of this membrane that even contributes to cell r ...
Hyojik Yang; Richard D. Smith; Courtney E. Chandler; J. Kristie Johnson; Shelley N. Jackson; Amina S. Woods; Alison J. Scott; David R. Goodlett; Robert K. Ernst
... We describe an innovative use for the recently reported fast lipid analysis technique (FLAT) that allows for the generation of MALDI tandem mass spectrometry data suitable for lipid A structure analysis directly from a single Gram-negative bacterial colony. We refer to this tandem MS version of FLAT as FLATⁿ. Neither technique requires sophisticated sample preparation beyond the selection of a sin ...
... Escherichia coli (E. coli) is the most common Gram-negative bacterium causing infection of the uterus or mammary gland and is one of the major causes of infertility in livestock. In those animals affected by E. coli driven LPS-mediated infections, fertility problems occur in part due to disrupted follicular and luteal functionality. However, the molecular mechanisms by which LPS induces inflammati ...
... Lameness is a significant problem for the UK sheep industry, accounting for approximately 65% of the industries total antibiotic use. Ovine footrot, the leading cause of infectious lameness, is a highly contagious polymicrobial bacterial infection, primarily caused by the Gram-negative bacterium Dichelobacter nodosus. Preventative antimicrobial footbaths are commonly used to reduce the spread of b ...
... Rho-dependent termination of transcription (RDTT) is a critical regulatory mechanism specific to bacteria. In a subset of species including most Actinobacteria and Bacteroidetes, the Rho factor contains a large, poorly conserved N-terminal insertion domain (NID) of cryptic function. To date, only two NID-bearing Rho factors from high G + C Actinobacteria have been thoroughly characterized. Both ca ...
... Titanium dioxide nanoparticles exhibit good anticancer and antibacterial activities. They are known to be environmentally friendly, stable, less toxic, and have excellent biocompatibility nature. Due to these properties, they are well suited for biological applications particularly in biomedical applications such as drug delivery and cancer therapy. In this research article, three medicinal herbs ...
Nicole Jackson; Clarissa A. Borges; Nicole J. Tarlton; Angel Resendez; Aubrianne K. Milton; Tara R. de Boer; Cheyenne R. Butcher; Niren Murthy; Lee W. Riley
... The increasing prevalence of extended spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC (pAmpC) β-lactamases among Enterobacterales threatens our ability to treat urinary tract infections (UTIs). These organisms are resistant to most β-lactam antibiotics and are frequently multidrug-resistant (MDR). Consequently, they are often resistant to antibiotics used to empirically treat UTIs. The lac ...
... E. coli and C. sakazakii are both opportunistic pathogenic bacteria that widely found in food. Emerging of antibiotic resistance makes the treatment of Gram-negative bacteria infection become ever more difficult. Therefore, it is urgent to find new antibacterial compounds against Gram-negative bacteria. Our previous study showed that bacteriocin BM1157 had broad antibacterial activity against both ...
... The acknowledgement of bioactive functions of compost teas promotes the research on characteristics and potential application of these heterogeneous water-soluble extracts from recycled biomasses. In this work, compost teas were isolated from on-farm composts made with agro-industrial residues of artichoke, pepper and coffee husks with the aim to evaluate the structural-activity relationship of di ...
... Chitosan is a nature-sourced polysaccharide widely used in numerous applications. The antibacterial potential of chitosan has attracted researchers to further develop and utilize this polymer for the formation of biocompatible antibacterial agents for both the food and healthcare industries. The tested hypothesis in this study is that modified N-alkylaminated chitosan nanoparticles (CNPs) have sel ...
... The drug resistance is higher among Gram-negative bacteria and demands the usage of strong antibiotics which can in turn result in systemic toxicity. In the treatment of the chronic wounds harboring pathogenic Gram-negative bacteria, the demand for an antimicrobial product that can be topically administered has been on the rise. In an effort to address the above issue, we have developed Colistimet ...
Sidra Aslam; Sajjad Ahmad; Fatima Noor; Usman Ali Ashfaq; Farah Shahid; Abdur Rehman; Muhammad Tahir ul Qamar; Eid A. Alatawi; Fahad M. Alshabrmi; Khaled S. Allemailem
... Chlamydia trachomatis, a Gram-negative bacterium that infects the rectum, urethra, congenital sites, and columnar epithelium of the cervix. It is a major cause of preventable blindness, ectopic pregnancy, and bacterial sexually transmitted infections worldwide. There is currently no licensed multi-epitope vaccination available for this pathogen. This study used core proteomics, immuno-informatics, ...
... Recently, different nanocrystals have been reported to be the alternative, optimistic, and novel antimicrobial agent against the many antibiotic‐resistant bacteria. Here, ligand‐free CdS and Ag‐doped CdS (Ag/CdS) nanocrystals have been synthesized by chemical methods for the study of the antimicrobial activity on Escherichia coli and Staphylococcus aureus by Kirby–Bauer diffusion method to see the ...
... Escherichia coli Nissle 1917 (EcN) is a Gram-negative bacterium that is used to treat inflammatory bowel diseases. The probiotic character of EcN is not well-understood, but its ability to produce secondary metabolites plays an important role in its activity. The EcN genome encodes for an aryl polyene (APE) biosynthetic gene cluster (BGC), and APE products have a role in biofilm formation. We show ...
... The existence of a live-chicken processing plant about 20 meters from a water source in Clarksville, Arkansas, raises concerns about bacteria contamination in the water. Over a period of three days, water samples from three different locations (upstream, across from the processing plant, and downstream) and for three different types of water at each location (running, stagnant in the middle, stagn ...
... Prevalence of widespread bacterial infections brings forth a critical need to understand the molecular mechanisms of the antibiotics as well as the bacterial response to those antibiotics. Improper use of antibiotics, which can be in sub-lethal concentrations is one among the multiple reasons for acquiring antibiotic resistance which makes it vital to understand the bacterial response towards sub- ...
... Probiotics are an eco-friendly and prospective substitute for antibiotic growth promoters. The present investigation aimed to dissect the probiotic attributes of a poultry isolate CGS1.1 by integrating genomics and phenotypic traits. The strain exhibited bile salt hydrolase activity and ∼73% survival in simulated gastrointestinal juices. It displayed notable aggregation, cell surface hydrophobicit ...
... Edwardsiella ictaluri is a Gram‐negative bacterium that has been reported to be disease causing in several economic fish including Nile tilapia. Here, immunoproteomic identification found that outer membrane protein A (OmpA) could react with serum from fish naturally infected with E. ictaluri. The expression and production of recombinant OmpA protein could be successfully conducted in Escherichia ...
Escherichia coli; Gram-negative bacteria; Salmonella Enteritidis; Salmonella Gallinarum; Salmonella Typhimurium; adhesion; chickens; colistin; macrophages; multiple drug resistance; plasmids; resistance genes; swine; virulence; China; South Korea
Abstract:
... Colistin is considered the last resort for the treatment of multi-drug resistant Gram-negative bacterial infections. We studied colistin resistance and the mcr-1 gene carriage in Salmonella isolates recovered from food animals in South Korea between 2010 and 2018. Colistin resistance was found in 277 isolates, predominantly in Salmonella Enteritidis (57.1%) and Salmonella Gallinarum (41.9%). Howev ...
Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Lewis acids; absorption; antifungal properties; chalcones; chemical structure; density functional theory; energy; fungi; green chemistry; hardness; nitrogen; research
Abstract:
... The present study explores a detailed comprehensive study on the computational, antibacterial and antifungal studies of pyrazoline derivatives. Four chalcones and corresponding 5-aryl-3-(4-fluorophenyl)-1-phenyl-4,5-dihydro-1H-pyrazoles were synthesized in PEG-400 and the structure of the pyrazolines were affirmed by IR, ¹H NMR, and ¹³C NMR spectral techniques. The PEG-400 mediated synthesis of py ...
... The photodynamic inactivation (PDI) of microorganisms has gained interest as an efficient option for conventional antibiotic treatments. Recently, Si(IV) phthalocyanines (SiPcs) have been highlighted as promising photosensitizers (PSs) to the PDI of microorganisms due to their remarkable absorption and emission features. To increase the potential of cationic SiPcs as PS drugs, one novel (1a) and t ...
Thi Mai Nguyen; Syaghalirwa N.M. Mandiki; Jean M.A.J. Salomon; Joel Bondekwe Baruti; Nang Thu Tran Thi; Thu Hang Nguyen; Truong Quynh Nhu; Patrick Kestemont
... Dietary lipids could modify fatty acid (FA) composition in fish tissues. Long chain polyunsaturated fatty acids (LC-PUFAs) such as arachidonic acid (ARA), eicosapentaneoic acid (EPA) and docosahexaenoic acid (DHA) are able to modulate the immune status in fish through an inflammatory process but their availability may be limited when fish are exclusively fed plant oils. This study was conducted to ...
... Calcium peroxide (CaO₂), a common solid peroxide, has been increasingly used in contaminated site remediation due to its ability to release oxygen (O₂) and hydrogen peroxide (H₂O₂) and its environmental friendliness. Our present study is first to explore micromechnisms of CaO₂ to efficaciously inactivate pathogen indicators including gram-negative bacterium of Escherichia coli (E. coli), gram-posi ...
... Infections caused by multidrug-resistant (MDR) bacteria, especially MDR Gram-negative bacteria, have posed a great challenge to healthcare systems globally. To address the shortage of effective antibiotics against MDR Gram-negative bacterial infections, two non-antibiotic drugs – auranofin (rheumatoid arthritis drug) and pentamidine (antiprotozoal drug) – are being repurposed to treat MDR Gram-neg ...
... Nucleic acid aptamers are target-specific oligonucleotides selected from combinatorial libraries through an iterative in vitro screening process known as Systemic Evolution of Ligands by Exponential Enrichment (SELEX). In this report, the selection of bacteria differentiating ssDNA aptamer candidates from a combinatorial library through the whole-cell SELEX method was performed. The enriched SELEX ...
... Background: Meningitis is associated with substantial morbidity and mortality, particularly in the first three months of life. Methods: We conducted a retrospective review of patients <90 days of age with meningitis at Texas Children’s Hospital from 2010–2017. Cases were confirmed using the National Healthcare Safety Network (NHSN) definition of meningitis. Results: Among 694 infants with meningit ...
... Fish‐derived antimicrobial peptides are an important part of the innate immune system due to their potent antimicrobial properties. Piscidins are a class of antimicrobial peptides first described in hybrid striped bass (Morone chrysops x Morone saxatilis) but have also been identified in many other fish species. Previous work demonstrated the broad antimicrobial activity of piscidins against Gram‐ ...
... Burkholderia pseudomallei is a Gram-negative bacterium and the causative agent of melioidosis in humans and animals in the tropics. The clinical manifestations of melioidosis are diverse, ranging from localized infections to whole-body sepsis. The effective serological method is crucial for the point-of-care diagnosis of melioidosis. The aim of this study was to develop indirect immunofluorescence ...
... Xanthogranulomatous pyelonephritis (XGP) represents a rare and severe pathology secondary to chronic urinary obstruction and recurrent infections. Commonly, this condition leads to loss of kidney function, and frequently, surgical approach is the only optional treatment. Proteus mirabilis and Escherichia coli are the most frequent pathogens associated with XGP. The actual changes in the pathogen’s ...
... An emerging need for new classes of antibiotics is, on the one hand, evident as antimicrobial resistance continues to rise. On the other hand, the awareness of the pros and cons of chemically synthesized compounds’ extensive use leads to a search for new metabolites in already known reservoirs. Previous research showed that Paenibacillus strain (P. alvei MP1) recovered from a b ...
Mohammad Y. Alshahrani; Zeeshan Rafi; Nadiyah M. Alabdallah; Ambreen Shoaib; Irfan Ahmad; Mohammed Asiri; Gaffar Sarwar Zaman; Shadma Wahab; Mohd Saeed; Salman Khan
... Rauwolfia serpentina (R. serpentina), belonging to the family Apocynaceae, is a renowned medicinal herb for its different pharmacological activities such as antibacterial, antifungal, anti-inflammatory, and antiproliferative characteristics. This study has done a comparative assessment of the antibacterial, antioxidant, and anti-cancer activity of R. serpentina aqueous leaf extract (RSALE) with en ...
... A novel Weissella cibaria strain UTNGt21O from the fruit of the Solanum quitoense (naranjilla) shrub produces a peptide that inhibits the growth of both Salmonella enterica subsp. enterica ATCC51741 and Escherichia coli ATCC25922 at different stages. A total of 31 contigs were assembled, with a total length of 1,924,087 bases, 20 contig hits match the core genome of different groups within Weissel ...
... Serine protease inhibitors (serpins) are a broadly distributed protease superfamily in various organisms, which participate in the negative regulation of melanization in insects. Our pervious study demonstrated that induced melanization by Heliothis virescens ascovirus 3h is helpful for HvAV-3h viral replication. In this study, the serpin-2.1 like gene from Spodoptera exigua (Seserpin-2.1 like) wh ...
... Galectins belong to the family of carbohydrate-binding proteins and play major roles in the immune and inflammatory responses of both vertebrates and invertebrates. In the present study, one novel galectin-1 protein named AjGal-1 was identified from Apostichopus japonicas with an open reading frame of 1179 bp encoding a polypeptide of 392 amino acids. The deduced amino acids sequence of AjGal-1 co ...
... Carbapenems, as the “last line of defense” against Gram-negative bacteria, are increasingly being challenged by drug-resistant bacteria, especially Enterobacteriaceae. In this study, a carbapenem-resistant Gram-negative bacterium, named AH001, was isolated from hospital sewage, and a modified Hodge test confirmed that this bacterium can produce carbapenemase. Further analysis revealed that this ba ...
... The present study evaluated the antimicrobial activities of acetic acid against bovine mastitis pathogens compared to lactic acid and lauric and caprylic saturated fatty acids. Eleven mastitis pathogens were isolated from sub-clinical and clinical bovine mastitis cases for the study. An initial screening of their antibacterial activities by agar well diffusion method was performed. The minimum inh ...
... Pigment from the endophyte Burkholderia sp. WYAT7 isolated from the medicinal plant Artemisia nilagirica (Clarke) Pamp. was extracted. The antibacterial efficacy of the crude pigment Y was assessed as a source of antibiotic against both Gram-positive and Gram-negative bacterial pathogens. The pigment Y exhibited a significant level of antibacterial activity against the tested pathogens Salmonella ...
... The emergence of the plasmid-mediated colistin resistance mechanism (mcr-1) makes bacterial resistance to colistin increasingly serious. This mcr-1 mediated bacterial resistance to colicin is conferred primarily through modification of lipid A in lipopolysaccharides (LPS). In our previous research, antimicrobial peptide F1 was derived from Tibetan kefir and has been shown to effectively inhibit th ...
... The antibacterial activity of methanolic extracts of eight Mediterranean brown algae belonging to the genera Cystoseira sensu lato, Dictyopteris and Padina was investigated. Extracts from Sargassaceae showed antibacterial activity. Specifically, Carpodesmia crinita and Carpodesmia brachycarpa extracts inhibited the growth of the Gram-positive strain Kocuria rhizophila whereas Cystoseira compressa ...
... Antimicrobial peptides (AMPs) play important roles in host innate immune systems. Anti-lipopolysaccharide factor (ALF), which is a primary AMP in crustaceans, is active against bacteria, fungi and some viruses. MjALF-D, an anionic peptide, is a group D ALF isolated from Marsupenaeus japonicus. In the present study, a series of experiments were performed to study its antibacterial spectrum and furt ...
... Mangrove plants are endowed with various biologically active compounds which have potent antibacterial and antioxidant properties. In present study, a bioactivity-guided fractionation for antibacterial and antioxidant active metabolites from the twigs of Avicennia officinalis collected from Kuala Selangor Nature Park, Selangor, Malaysia gave 13 major fractions. The antibacterial activity of A. off ...
... Infections by multidrug-resistant (MDR) bacteria are one of the most threatening concerns for public health. For this purpose, nanomaterials have emerged with great potential for antibacterial activity. In this paper, we report the synthesis of new Ni²⁺-doped zinc oxide (Ni-ZnO or NZO) nanostructures as targeted antibacterial agents for Gram-negative bacteria. A one-pot low-temperature solution pr ...
... Copolymer brushes with different ratios of sulfobetaine methacrylate (SBMA) and [2-(Acryloyloxy)ethyl]trimethylammonium chloride (DAC) were grafted from transparent cellulose membrane (CM) via surface-initiated atom transfer radical polymerization (SI-ATRP) method for improving its antifouling and antibacterial performance. Surface concentrated copolymer grafting on the cellulose membranes can be ...
... OBJECTIVE: To evaluate in vitro the antibacterial effects of fluorescein, rose bengal, and lissamine green topical ophthalmic dyes against selected Gram‐positive and Gram‐negative bacteria, and to evaluate whether preserved or preservative‐free fluorescein solutions are able to inhibit or potentiate bacterial growth. PROCEDURES: Susceptibility testing was performed using the Kirby‐Bauer disk diffu ...
T. Stalin Dhas; P. Sowmiya; V. Ganesh Kumar; M. Ravi; K. Suthindhiran; J. Francis Borgio; G. Narendrakumar; V. Ramesh Kumar; V. Karthick; C.M. Vineeth Kumar
... Biosynthesis of gold nanoparticles has been carried out with various sources like bacteria, plants and algae. Seaweeds have high nutritional and medicinal properties. Sargassum plagiophyllum is seaweed that has excellent antioxidant activity. This study aims at evaluating the antimicrobial activity of biosynthesized gold nanoparticles from the extract of S. plagiophyllum. The synthesised nanoparti ...
... In the current work, antimicrobial bionanocomposite films were fabricated based on chitosan (CS), poly (vinyl alcohol) (PVA) and nanocomposites comprised of silicon dioxide nanoparticles doped with zinc oxide nanoparticles (ZnO-SiO₂). The ZnO-SiO₂ nanocomposite was prepared and incorporated into a PVA/CS blend at different ratios (0.50, 1.0, 3.0 and 5.0 %) to generate the PVA/CS/ZnO-SiO₂ bionanoco ...
Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Klebsiella; Staphylococcus; amikacin; ampicillin; antibiotic resistance; ceftazidime; dog diseases; dogs; erythromycin; females; florfenicol; hospitals; males; meropenem; multiple drug resistance; nitrofurantoin; pathogens; polymyxins; pulsed-field gel electrophoresis; therapeutics; urinary tract diseases; vancomycin; China
Abstract:
... Urinary tract infections (UTIs), many of which are caused by bacterial pathogens, are some of the most common infections in dogs. To effectively treat UTIs, it is important to identify the predominant bacterial pathogens and their susceptibility to antimicrobial agents. In this study, we collected 326 samples from cases with UTIs or other urinary system diseases at the China Agricultural Universit ...
... Due to the environmental problems caused by the use of synthetic plastics in food packaging, it is necessary to replace them with biodegradable films. In this study, carboxyl methyl cellulose/gelatin composite modified with TiO₂–Ag nanoparticles (CMC/Gel/TiO₂–Ag) was prepared and its antioxidant, antimicrobial, mechanical, colorimetric and morphological characteristics were investigated. To evalua ...