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... Global interest in phytogenic feed additives as alternatives to antibiotics in feed has been spurred by the banning of antibiotic growth promoters by several countries. Suitable plant extracts for development of phytogenic feed additives should have therapeutic value and should also be safe. The aim of this study was to evaluate the antibacterial, antioxidant and anti-lipoxygenase activities as we ...
... The antimicrobial properties of essential oils from spice and medicinal plants have aroused interest for their potential as an alternative to the use of chemical preservatives in foods. In this work, we studied the bacteriostatic and bactericidal effects of Oregano, Thyme, Clove, Cinnamon and Black-pepper essential oils (EOs) against strains of Staphylococcus aureus, Listeria sp, Escherichia coli ...
COVID-19 infection; Escherichia coli; World Health Organization; environment; gastrointestinal system; health hazards; hygiene; ingestion; microbial load; microbiological quality; microbiological risk assessment; models; pandemic; probability; recreation; sanitation; surface water; wastewater treatment; water pollution; watersheds; South Africa
Abstract:
... The World Health Organization reported that COVID-19 cases reached 611,421,786 globally by September 23, 2022. Six months after the first reported case, the disease had spread rapidly, reaching pandemic status, leading to numerous preventive measures to curb the spread, including a complete shutdown of many activities worldwide. Such restrictions affected services like waste management, resulting ...
... This research was directed to understand the bactericidal effect of epidermal mucus of two Asian cat fish species viz. Clarias batrachus and Heteropneustes fossilis. Epidermal mucus extracts (raw and diluted) of both cat fish species were tested against several Gram negative (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia, A. hydrophila) and Gram positive bacterial strains (Bacilli ...
... Bdellovibrio are obligate predators of Gram-negative bacteria that could parasitize other bacteria and cause their lysis. It has been considered as a potential probiotic and may replace antibiotics. In order to better explore the inhibitory activity of Bdellovibrio against common aquatic pathogens and its potential use in aquaculture, the Aeromonas hydrophilia was used as the host bacteria for cul ...
... Hexabromocyclododecane (HBCD) is a typical persistent organic pollutant that is widely detected in the environment. Despite the significant efforts put into its mineralisation, there is still a lack of microorganism resources that can completely mineralise HBCD. Stable isotope analysis revealed that the Citrobacter sp. Y3 can use [¹³C]HBCD as its sole carbon source and degrade or even mineralise i ...
Acinetobacter calcoaceticus; Burkholderia mallei; Escherichia coli; Klebsiella pneumoniae; Mycobacterium tuberculosis; Pseudomonas aeruginosa; bacterial communities; corrosion; cytotoxicity; environment; pathogens; perfluorocarbons; phthalates; risk; tap water; water distribution; water treatment; China
Abstract:
... The adverse health effects of phthalate esters (PAEs) and perfluoroalkyl substances (PFAS) in drinking water have attracted considerable attention. Our study investigated the effects of PAEs and PFAS on the bacterial community and the growth of potential human pathogenic bacteria in rural drinking water distribution systems. Our results showed that the total concentration of PAEs and PFAS ranged f ...
... Two kinds of N-hydrophobic-O-hydrophilic chitosan derivatives, N-2-hydroxypropyl-3-butyl ether-O-carboxymethyl chitosan (HBCC) and N-2-hydroxypropyl-3-(2-ethylhexyl glycidyl ether)-O-carboxymethyl chitosan (H2ECC) were synthesized and cross-linked with glutaraldehyde to prepare HBCC and H2ECC films. The properties of the cross-linked films, including microstructure, mechanical properties, water va ...
... As a new type of environmental pollutant, antibiotic resistance genes (ARGs) pose a huge challenge to global health. Horizontal gene transfer (HGT) represents an important route for the spread of ARGs. The widespread use of sulfamethazine (SM2) as a broad-spectrum bacteriostatic agent leads to high residual levels in the environment, thereby increasing the spread of ARGs. Therefore, we chose to st ...
... Formate hydrogenlyase-1 (FHL-1) is a complex-I-like enzyme that is commonly found in gram-negative bacteria. The enzyme comprises a peripheral arm and a membrane arm but is not involved in quinone reduction. Instead, FHL-1 couples formate oxidation to the reduction of protons to molecular hydrogen (H₂). Escherichia coli produces FHL-1 under fermentative conditions where it serves to detoxify formi ...
... We conducted a study on the antibacterial effect of dielectric barrier discharge plasma (DBDP) treatment on Escherichia coli and Vibrio parahaemolyticus, which cause food poisoning on the surface of polypropylene (PP) plastic containers. The initial concentrations of E. coli on PP plastic surfaces were 7.02 log CFU/coupon, and 6.50–5.37 log CFU/coupon in 5–60 min of DBDP treatment, respectively. I ...
... Pseudomonas fragi and Escherichia coli are considered as common colonizers of fresh and spoilage meat, where they tend to live in the proximity. In this study, we primarily tested interplay patterns between different isolates of these two species in two-by-two combinations grown on stainless steel surfaces as dual-species biofilms. Results showed that these two species presented competition as maj ...
... A range of spreads and dips as novel vegetarian ready-to-eat foods are being introduced on the (European) retail market to response to an increasing number of vegetarian’/flexitarian’ consumers and the shift towards plant-based foods consumption. This innovative food group was explored by a product survey (n = 369) in Belgium. The spreads and dips were classified on the presence of main ingredient ...
... This study fabricated yellow-emitting CDs (Y-CDs) by hydrothermal treatment of citric acid and urea and applied them as a fluorescence turn-on platform for sensitive and selective detection of lipopolysaccharide (LPS) based on the non-shifted AIEE of peptide-stabilized CD aggregates. The designed peptide (named K3) consisting of aggregation-active and LPS-recognition units triggered the aggregatio ...
... The aim of this work is to study the immune responses of the polychaete Sabella spallanzanii after exposure to copper sulphate, an immunomodulating agent in marine organisms, and the multiple stresses caused by Escherichia coli infection, to validate the species as a model organism in marine-coastal biomonitoring programmes. Polychaetes were housed in laboratory and divided into five experimental ...
... This is the first report on a polysaccharide (RCJ2-Ib) isolated from Rosa Chinensis Flos. RCJ2-Ib was obtained through the extraction with water, precipitation with ethanol, separation with DEAE-52 column and purification with DEAE-Sepharose Fast Flow column and Sephadex G100 column. GC, FT-IR and NMR analyses revealed that RCJ2-Ib (3.3 kDa) was a 1,4-linked polymannuronic acid containing substant ...
... The regular release kinetics of active ingredients is inconsistent with the demand of meat preservation. Herein, the pH-triggered dynamic mechanism of packaging film was developed based on L100 polymer incorporated with Cinnamon essential oil (CEO) by coaxial electrospinning. The acquired results revealed that UV–vis and fluorescence spectra confirmed the pH release behavior. The fabricated film w ...
... Air ionizers and 222-nm krypton-chlorine (KrCl) excilamp have proven to be effective disinfection apparatus for bacteria and viruses with limited health risks. We determined inactivation efficiencies by operating them individually and in combined modules. Gram-positive and gram-negative bacteria, non-enveloped dsDNA virus, and enveloped dsRNA virus were examined in a designed air disinfection syst ...
DNA; Escherichia coli; World Health Organization; air; air flow; bioaerosols; cell membranes; hospitals; models; osmotic pressure; relative humidity; research; saliva; urine; wastewater
Abstract:
... The composition and concentration of airborne microorganisms in hospital indoor air has been reported to contain airborne bacteria and fungi concentrations ranged 10¹–10³ CFU/m³ in inpatients facilities which mostly exceed recommendations from the World Health Organization (WHO). In this work, a deeper knowledge of the performance of airborne microorganisms would allow improving the designs of the ...
... Stormwater reuse is one of the most important ways to mitigate water resource shortage. However, urban stormwater contains many bacteria species, which threaten the reuse safety. Therefore, stormwater disinfection is highly needed. Although disinfection has been widely conducted in the drinking water and reclaimed water, it is rarely carried out for stormwater. This study collected the roof stormw ...
... Invertebrates' immune priming or innate immune memory is an analogous response to the vertebrates' adaptive memory. We investigated if honey bees have immune memory. We compared survival and immune response between bees that were: 1) manipulated (Naïve), 2) challenged twice with the same pathogen Escherichia coli (Memory), 3) challenged twice with different pathogens (Staphylococcus aureus versus ...
Escherichia coli; Scutellaria baicalensis; active ingredients; anti-inflammatory activity; anticoagulants; apigenin; baicalin; bioactive compounds; coagulation; eugenol; herbal medicines; palmitic acid; pharmacodynamics; quality control; traditional medicine
Abstract:
... Bioactive substance identification is always the focal point and the main challenge in Chinese herbal medicine (CHM). Most CHM present multiple efficacies and multiple tropisms, which has improved the application accuracy of CHM, and is worthy of further study. In this article, the concept of “multi-tropism efficacy of CHM” has been proposed for the first time. In addition, it is hypothesized that ...
... Two imine derivatives of chitosan (i-CTs), namely 2FCT and 5MCT, were synthesized by reacting chitosan (CT) with 2-(3-formyl-1H-indol-1yl)acetonitrile (2F), and 5-methoxyindole-3-carbaldehyde (5M), respectively. The antimicrobial evaluation of i-CTs exhibited stronger inhibition effect against Staphylococcus aureus, Escherichia coli and Candida albicans. The antioxidant activity of 2FCT and 5MCT s ...
... A new protein detector of 2,4,6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of β-lactamase and, subsequently, expressed in the periplasmic space of Escherichia coli, affording in such a way the recombinant fusion protein 3.5-scFv-β-lactamase. This bifunctional immun ...
... Nanofibrous dressings exhibit high specific surface areas, good histocompatibility, enhanced wound healing, and reduced inflammation, which have broad technological implications for treating diabetic foot ulcers (DFUs). However, current nanofibrous dressings still suffer from high resistance to cell infiltration and multiple dressing changes. In this study, polycaprolactone (PCL) and collagen were ...
... Currently, a suitable bioink for 3D bioprinting and capable of mimicking the microenvironment of native skin and preventing bacterial infection remains a major challenge in skin tissue engineering. In this study, we prepared a tissue-specific extracellular matrix-based bioink, and dECM/Gel/QCS (dGQ) 3D scaffold assembling with poly(ionic liquid)s (PILs) (dGQP) was obtained by an extrusion 3D biopr ...
... Direct 3D bioprinting of bioreactors containing microorganisms embedded inside hydrogel structures is a promising strategy for biotechnological applications. Nevertheless, microporous hydrogel networks hinder the supply of nutrients and oxygen to the cell and limit cell migration and proliferation. To overcome this drawback, we developed a feedstock for 3D bioprinting structures with hierarchical ...
... Three-dimensional (3D) printing technology has received remarkable attention in manufacturing catalysts with tailored shapes and high precision, particularly facilitating catalyst recovery, maximizing heat/mass transfer, as well as enhancing catalytic performance. Herein, an engineered recombinant Escherichia coli strain (denoted as e-E. coli) with overexpressing metallothionein (a metal-binding p ...
... This study demonstrates a novel multi-functional microfluidic system, designated three dimensional Alternative Current Electrokinetic/Surface Enhanced Raman Scattering (3D-ACEK/SERS), which can concentrate bacteria from whole blood, identify bacterial species, and determine antibiotic susceptibilities of the bacteria rapidly. The system consists of a hybrid electrokinetic mechanism, integrating AC ...
... Nitroreductases activate nitroaromatic antibiotics and cancer prodrugs to cytotoxic hydroxylamines and reduce quinones to quinols. Using steady‐state and stopped‐flow kinetics, we show that the Escherichia coli nitroreductase NfsA is 20–50 fold more active with NADPH than with NADH and that product release may be rate‐limiting. The crystal structure of NfsA with NADP⁺ shows that a mobile loop form ...
... In order to develop better wound dressings, a novel chitosan hydrogel (Cn-Nm gel) was designed and fabricated by using aldehyde-4-arm polyethylene glycol (4r-PEG-CHO) to crosslink the chitosan dissolved in alkaline solution, amino-4-arm polyethylene glycol (4r-PEG-NH₂) was chosen as the additive simultaneously. The special dissolution technique and macromolecular crosslinking structure endows the ...
... OBJECTIVES: To investigate the level of 5-aminolevulinic acid (5-ALA), a key precursor of heme, and expression of heme-peroxidase on the regulation of heme synthesis in E. coli. METHODS: A transporter gene (eamA) was knocked out, and glutamyl-tRNA reductase gene (hemA) for 5-ALA synthesis and a dye-decolorizing peroxidase gene (DyP) were overexpressed. RESULTS: Knockout of eamA caused decrease of ...
... Until recently, the cyanobacterial phylum only included oxygenic photosynthesizer members. The discovery of Melainabacteria as a group of supposed non-photosynthetic cyanobacteria asked to revisit such scenario. From metagenomic data, we were able to identify sequences encoding putative ADP-glucose pyrophosphorylases (ADP-GlcPPase) from free-living and intestinal Melainabacteria. The respective ge ...
... Escherichia coli (E. coli) are common bacteria that colonize the human and animal gastrointestinal tract, where they help maintain a balanced microbiome. However, some E. coli strains are pathogenic and can cause serious infectious diseases and life-threatening complications. Due to the overuse of antibiotics and limited development of novel antibiotics, the emergence of antibiotic-resistant strai ...
... ATP plays an essential role in the substrate/product transmembrane transportation during whole-cell bioconversion. This study aimed to address the impact of ATP upon cadaverine synthesis by whole-cell biocatalysts. The results showed no significant change in the ATP content (P = 0.625), and the specific cadaverine yield (P = 0.374) was observed in enzyme-catalyzed cadaverine synthesis with exogeno ...
Escherichia coli O157; Salmonella Typhimurium; Shiga toxin; apple juice; chickens; detection limit; droplets; fruits; human health; meat; milk
Abstract:
... Shiga-toxin producing Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium are important foodborne pathogens which are threatened to human health seriously. In this study, we firstly investigated the application and accuracy of next-generation sequencing (NGS) technology combining with droplet digital PCR (NGS + ddPCR) for the quantification of pathogenic bacteria through amplifica ...
... Acid hydrolysis of sucrose was studied as a method to convert sucrose in sweet sorghum syrup into glucose and fructose before fermentation with the genetically engineered succinic acid-producing Escherichia coli strain AFP184. Successful hydrolysis of sucrose in sweet sorghum syrup was achieved without noticeable formation of degradation products using moderate temperatures, 65–85 °C, with 0.24–0. ...
... To meet the demands for more effective and ecofriendly food packaging strategies, the potential of nisin-loaded rhamnolipid functionalized nanofillers (rhamnosomes) has been explored after embedding in hydroxypropyl-methylcellulose (HPMC) and κ-carrageenan (κ-CR)-based packaging films. It was observed that intrinsically active rhamnosomes based nanofillers greatly improved the mechanical and optic ...
... The aim of this study was to develop and characterize sodium alginate films loaded with 10–40 % Clitoria ternatea extract (CTE) and apply to monitoring the quality of milk, pork and shrimp. Films loaded with CTE showed high light barrier capacity and improved tensile strength by 3.8 times over control films. The incorporation of CTE in alginate films improved the thermal stability of the materials ...
... Multi-nutrient starvation profiles are emerging as an essential feature of human pathogen studies. In the present work, we attempted to understand why V. cholerae cannot survive in the growth media that includes carbon and nitrogen but lacks phosphate. However, it can survive starvation in artificial seawater without all three major nutrients (carbon, nitrogen, and phosphate). V. cholerae survival ...
Michael O. Rivett; Laurent-Charles Tremblay-Levesque; Ruth Carter; Rudi C.H. Thetard; Morris Tengatenga; Ann Phoya; Emma Mbalame; Edwin Mchilikizo; Steven Kumwenda; Prince Mleta; Marc J. Addison; Robert M. Kalin
Escherichia coli; environment; groundwater; hygiene; infrastructure; issues and policy; sanitation; statistical analysis; surveys; water quality; water table; wet season; Malawi
Abstract:
... This longitudinal flood-relief study assessed the impact of the March 2019 Cyclone Idai flood event on E. coli contamination of hand-pumped boreholes in Mulanje District, Malawi. It established the microbiological water-quality safety of 279 community supplies over three phases, each comprising water-quality survey, rehabilitation and treatment verification monitoring. Phase 1 contamination three ...
Escherichia coli; coagulation; disinfection; electrochemistry; filtration; ion exchange; nitrates; surface water
Abstract:
... In previous works, a low-cost predisinfection column that combined coagulation-flocculation and GAC filtration was proposed for combination with electrodisinfection in the successful treatment of highly faecal polluted surface water. In this work, this column is adapted for the treatment of pore water by transforming the coagulation chamber into a chemical reactor with lime and replacing the GAC o ...
... Previously, Escherichia coli was engineered to produce isobutyl acetate (IBA). Titers greater than the toxicity threshold (3 g/L) were achieved by using layer-assisted production. To avoid this costly and complex method, adaptive laboratory evolution (ALE) was applied to E. coli for improved IBA tolerance. Over 37 rounds of selective pressure, 22 IBA-tolerant mutants were isolated. Remarkably, the ...
Escherichia coli; carbon; energy density; enzyme kinetics; evolutionary adaptation; feedstocks; fermentation; gamma-aminobutyric acid; glycerol; mutants; mutation; transcriptomics; value added
Abstract:
... The microbial conversion of glycerol into value-added commodity products has emerged as an attractive means to meet the demands of biosustainability. However, glycerol is a non-preferential carbon source for productive fermentation because of its low energy density. We employed evolutionary and metabolic engineering in tandem to construct an Escherichia coli strain with improved GABA production us ...
... The adhesion and aggregation are characteristic attributes of probiotic strains belonging to Lactobacillaceae genus. Due to these properties the host organisms can avoid colonisation of the intestinal tract by enteropathogenic bacteria. The presented research includes a comparison of the properties of various strains belonging to different Lactobacillaceae species and isolated from different sourc ...
Escherichia coli; absorption; adsorbents; adsorption; antibacterial properties; cadmium; clay; cost effectiveness; heavy metals; irradiation; light; photocatalysis; photocatalysts; sepiolite; surface area; value added; wastewater; water purification
Abstract:
... High value-added recycling of hazardous substances emerges as one of the most promising directions in current society, which can simultaneously relieve the environmental burden and obtain useful products. Here, we propose a transformation strategy from adsorbent to photocatalyst by recycling heavy metal with natural clay mineral. Sepiolite is selected as an adsorbent for removing Cd²⁺ in wastewate ...
... The inactivation of antibiotic resistant bacteria (ARB) and genes (ARGs) in an advanced plant combining ozonation and granular activated carbon (GAC) filtration applied for effluent after conventional activated sludge treatment at a full-scale urban wastewater treatment plant was investigated for over 13 consecutive months. The nitrite compensated specific ozone dose ranged between 0.4 and 0.7 g O ...
... As an important raw material for pharmaceutical, food and feed industry, highly efficient production of L-tryptophan by Escherichia coli has attracted a considerable attention. However, there are complicated and multiple layers of regulation networks in L-tryptophan biosynthetic pathway and thus have difficulty to rewrite the biosynthetic pathway for producing L-tryptophan with high efficiency in ...
... Cavitation treatment of the mixture of peat and water makes it possible to obtain peat gel that is saturated with active water-soluble forms of humic acids. This work shows the advantages of peat gel in comparison with untreated peat for reducing the toxicity of soils polluted with oil products. Gasoline was added to the soddy-podzolic soil: 1, 5, and 10% of the dry soil. In one series of experime ...
... Aeromonas veronii AvX005 is a pathogenic bacterium with high toxicity to grass carp (Ctenopharyngodon idellus). The expression levels of g-type (goose-type lysozyme, Lys-g) and c-type lysozyme (chicken-type lysozyme, Lys-c) in the spleen of grass carp infected with AvX005 were significantly increased by approximately 4.5 times and 27 times, respectively. The recombinant proteins rLys-g and rLys-c ...
... Antibiotic resistance poses a threat in many institutions including hospitals, meat processing facilities and other agricultural and occupational entities. The goal of this research is to uncover how bacteria react to environmental conditions including aerosolization and delineate factors that trigger expression of antibiotic resistance genes (ARGs) using the laboratory strain Escherichia coli (E. ...
... In this research, the extraction conditions of natural dyes from Sarsaparilla root were analyzed by response surface method, and the interaction between extract factors and their significant effects on sarsaparilla root extraction efficiency were discussed. The main components of Sarsaparilla root extraction were investigated by ATR-FTIR and chromogenic reaction, and the stability results of Sarsa ...
... AIMS: To gather data on agricultural practices in organic farms in Sao Paulo, Brazil, and evaluate their relationship with the microbiological characteristics of samples collected along the production chain. METHODS AND RESULTS: Practices data were based on field observations and interviews with farmers in 10 selected organic lettuce producing farms. Counts of Enterobacteriaceae and surveys for Sa ...
... Among factors that can affect calf health, microbial quality of the pen air is poorly studied. In 25 Italian dairy farms, data concerning air quality in the calf pens, hygiene of pens and equipment, microclimatic conditions, calf health and management were collected during the winter season (January-March 2020 and December-March 2021). The average air Standard Plate Count (SPC) of 85 pens was 4.51 ...
Escherichia coli; alginates; antibacterial properties; antifungal properties; antioxidants; breads; chronic exposure; cytotoxicity; fibroblasts; functional foods; fungi; hot water treatment; mice; modulus of elasticity; permeability; polymers; sulfur; tensile strength; water vapor
Abstract:
... Sulfur quantum dots (SQDs) were fabricated using a facile hydrothermal method and used for the preparation of functional food packaging film and compared the properties with other sulfur-based fillers like elemental sulfur (ES) and sulfur nanoparticles (SNP). The SQDs have an average size of 5.3 nm and were very stable in aqueous suspension. Unlike other sulfur-based fillers, the SQD showed high a ...
Escherichia coli; amino acids; analytical chemistry; chemical reactions; crosslinking; guidelines; hydrazides; mass spectrometry; protein structure
Abstract:
... The coverage of chemical crosslinking coupled with mass spectrometry (CXMS) is of great importance to determine its ability for deciphering protein structures. At present, N-hydroxysuccinimidyl (NHS) ester-based crosslinkers targeting lysines have been predominantly used in CXMS. However, they are not always effective for some proteins with few lysines. Other amino acid residues such as carboxyl c ...
Escherichia coli; biocatalysis; biorefining; byproducts; carbon; coproducts; green chemistry; oleic acid; space and time; sustainable technology
Abstract:
... Oxyfunctionalization of renewable oleic acid is an environmentally friendly process for producing C9 bifunctional chemicals, i.e., 9-hydroxynonanoic acid and its derivatives. However, the carbon atom economy of 9-hydroxynonanoic acid is extremely low (50%) due to the unavoidable formation of the unifunctional n-nonanoic acid as a coproduct. In this work, to further enhance the feasibility of its a ...
... Escherichia coli (E. coli) strains cause the majority of urinary tract infections (UTIs) and are resistant to various antibiotics. Therefore, it is imperative to explore novel host-target therapies. As a famous food and condiment, garlic (Allium sativum L.) is widely used in medicine, but its exact key targets in UTIs remain elusive. To identify the major active ingredient of garlic and its molecu ...
Escherichia coli; biotechnology; biotransformation; drug therapy; formate dehydrogenase; mass spectrometry; polarimetry; psicose; sugar alcohols
Abstract:
... As a kind of rare sugar alcohol, allitol has important application values in food and medication. In addition, it can be used as a key substrate to produce other d/l-rare sugars. Allitol can be effectively produced by the resting-cell biotransformation method.Two recombinant Escherichia coli strains, one simultaneously expressing ribitol dehydrogenase (RDH) and formate dehydrogenase (FDH) in fusio ...
... Biomedical implants-associated bacterial infections have become a major threat to human health. Therefore, it is meaningful to develop new antibacterial strategies to solve this problem. In this study, we conjugated acetylated lentinan (AceLNT) with α-terpineol (AceLNT-g-α-ter), a highly effective natural antibacterial compound, to constitute a novel AceLNT-g-α-ter membrane (AceLNT-g-α-terM). Comp ...
... Pest infestation in any stage can lead to a quality reduction in the finished products. This study aimed to detect Escherichia coli, Salmonella spp., Campylobacter spp., and Staphylococcus aureus in Alphitobius diaperinus adults, and in samples from broiler swabs, administered water and feed collected in a single house from a broiler production facility in central Italy. Three samplings were carri ...
... The gut microbiota consists a diverse and complex ecosystem that is involved in beneficial functions as well as potentially harmful conditions for human. Blastocystis sp. is a common parasite of the digestive tract of animals and humans; however, limited data is available concerning the association of asymptomatic Blastocystis infection and gut bacteria composition. Hence, in this cross-sectional ...
Bifidobacterium; Clostridium; Escherichia coli; Gallus gallus; Lactobacillus; NAD (coenzyme); biomarkers; cecum; chlorides; gene expression; interleukin-6; microvilli; nicotinamide; water solubility
Abstract:
... Nicotinamide riboside (NR) acts as a nicotinamide adenine dinucleotide (NAD+) precursor where NR supplementation has previously been shown to be beneficial. Thus, we synthesized and characterized nicotinamide riboside tributyrate chloride (NRTBCl, water-soluble) and nicotinamide riboside trioleate chloride (NRTOCl, oil-soluble) as two new ester derivatives of nicotinamide riboside chloride (NRCl). ...
Escherichia coli; bone marrow; chickens; gene expression regulation; gene ontology; genes; immune response; microRNA; quantitative polymerase chain reaction; research; reverse transcription; specific pathogen-free animals; transcriptome; veterinary medicine
Abstract:
... Avian pathogenic Escherichia coli (APEC) is one of the most common avian bacterial diseases globally. The bone marrow is a reservoir of immature immune cells. To elucidate the role of bone marrow microRNAs (miRNAs) in regulating the host response to APEC infection, we performed miRNA-seq to investigate alterations in the expression of bone marrow miRNAs in three groups of specific pathogen-free ch ...
... To prevent infections associated with biomedical catheters, various antimicrobial coatings have been investigated. However, those materials do not provide consistent antibacterial effects or biocompatibility, generally, due to degradation of the coating materials, in vivo. Additionally, biomedical catheters must have low surface friction to reduce tribological damage. In this study, we developed a ...
... This is a report on the encapsulation amoxicillin (AMX) in the N-2-Hydroxypropyl trimethyl ammonium chloride chitosan (N-2-HACC) and N,O-carboxymethyl chitosan (CMCS) nanoparticles (NPs) for biomedical applications. The N-2-HACC/CMCS NPs have broad-spectrum antibacterial properties. In order to achieve sustained and slow drug release, improve drug transport efficiency and bioavailability, prolong ...
... In the historical mysteries and present pandemic situation, the use of citrus fruits makes it rise high among other fruits. Citrus has a significant role in dietary and medicinal purposes from time immemorial and widely acknowledged for its therapeutic properties. Citrus megaloxycarpa Lush. is an unspecified sibling of the citrus family. The present work highlights the biochemical, antimicrobial, ...
... Family I.3 lipase is distinguished from other families by the amino acid sequence and secretion mechanism. Little is known about the evolutionary process driving these differences. This study attempt to understand how the diverse temperature stabilities of bacterial lipases from family I.3 evolved. To achieve that, eighty-three protein sequences sharing a minimum 30% sequence identity with Antarct ...
... Antibiotic resistance genes (ARGs) in municipal drinking water may not be effectively removed during centralized treatment. To reduce potential health risks, water disinfection at the point-of-use scale is warranted. This study investigated the performance of boiling, a prevalent household water disinfection means, in response to ARGs contamination. We found that boiling was more efficient in inac ...
... Anopheles gambiae and Anopheles coluzzii are closely related species that are predominant vectors of malaria in Africa. Recently, A. gambiae form M was renamed A. coluzzii and we now conclude on the basis of a diagnostic PCR‐restriction fragment length polymorphism assay that Ag55 cells were derived from A. coluzzii. We established an Ag55 cell transcriptome, and KEGG pathway analysis showed that ...
Amir Mahgoub Awadelkareem; Eyad Al-Shammari; AbdElmoneim O. Elkhalifa; Mohd Adnan; Arif Jamal Siddiqui; Danish Mahmood; Z. R. Azaz Ahmad Azad; Mitesh Patel; Khalid Mehmood; Corina Danciu; Syed Amir Ashraf
... Bacterial cells have the ability to form biofilm onto the surfaces of food matrixes and on food processing equipment, leading to a source of food contamination posing serious health implications. Therefore, our study aimed to determine the effect of Eruca sativa Miller (E. sativa) crude extract against biofilms of food-borne bacteria along with in silico approaches to investigate adhesion proteins ...
Bacteroides; Bifidobacterium; Escherichia coli; Lactobacillus; anti-aging properties; antioxidant activity; body weight changes; dietary fiber; eating habits; hippocampus; human health; humans; inflammation; intestines; longevity; memory; necrosis; neurons; spleen; China
Abstract:
... A series of previous studies by our team has shown that the Guangxi longevity dietary pattern contributes to the improvement of human health, but the role of dietary fiber compounds (DFC) in the anti-aging of this dietary pattern has not been studied in depth. Thus, mice were fed with 5%, 15%, and 30% of the characteristic dietary fiber compound (CDFC) (compounded according to the longevity dietar ...
... In previous research works, we have described the production of 7 kDa F5 peptide after a hydrolysis of the white shrimp, Metapenaeus monoceros (Fabricius, 1798) by-product, using a serine alkaline protease (SPVP) purified from Aeribacillus pallidus strain VP3. The present study aims to explore the antioxidative potentials of F5 peptide by both in vitro and in vivo assays. The anti-biofilm activity ...
Maria R. Xavier; Thiago S. Freitas; Raimundo L.S. Pereira; Emanuelle M. Marinho; Paulo N. Bandeira; Amanda P. de Sousa; Larissa S. Oliveira; Lucas Lima Bezerra; José B.A. Neto; Maria M.C. Silva; Beatriz G. Cruz; Janaína E. Rocha; Cristina R.S. Barbosa; Antonio W. da Silva; Jane E.S.A. de Menezes; Henrique D.M. Coutinho; Márcia M. Marinho; Emmanuel S. Marinho; Hélcio S. dos Santos; Alexandre M.R. Teixeira
... The prevalence of multidrug-resistant (MDR) bacteria and the limited efficacy of current available antibiotics cause every year approximately 700 000 deaths per year. This study aimed to evaluate the anti-inflammatory effect and antibacterial potential of the ibuprofen derivative Methyl 2-(-4-isobutylphenyl)propanoate (MET-IBU). The molecular structure of MET-IBU was confirmed by Nuclear Magnetic ...
Alejandro Martínez-Ceja; Antonio Romero-Estrada; María Crystal Columba-Palomares; Israel Hurtado-Díaz; Laura Alvarez; Rubicela Teta-Talixtacta; Mariana Sánchez-Ramos; Francisco Cruz-Sosa; Antonio Bernabé-Antonio
... Randia aculeata (Rubiaceae) is a medicinal plant distributed in the state of Jalisco, Mexico. In folk medicine it is used to counteract the effects produced by the bite of snakes and other poisonous insects. However, there are no reports of new alternatives to use the plant in a sustainable way. The main objective of this study was to establish a cell culture of R. aculeata, evaluate its anti-infl ...
... Nanoparticle materials have received increasing attention in the functional modification of textiles. In this work, pure TiO₂, Ag-doped TiO₂, Fe-doped TiO₂, and graphene oxide nanoparticles were used to impart the anti-bacterial and adsorptive properties of nanoparticles to cotton fabric. The treated fabric materials were investigated by X-ray diffraction, Fourier transform infrared spectroscopy, ...
... The increase in cases of antibiotic resistance and the discoveries cessation of new classes of antibiotics encourages the exploration of various microorganism sources from unique environments to produce antimicrobial compounds. This study aimed to characterize actinobacteria isolated from marine sediment and evaluate the best medium and incubation time for its antibacterial activities. The sedimen ...
... Many studies have demonstrated that natural plant extracts have inhibitory effects on microorganisms. The purpose of this study was to investigate the inhibitory effect of phenolic acids from rice straw (PAs) on Escherichia coli and their synergistic effect in combination with antibiotics. PAs can inhibit the growth of E. coli effectively by inducing the formation of H₂O₂; PA-treated cells had a t ...
... The antimicrobial activity of Allium ursinum aqueous extract prepared using high pressure extraction was evaluated. Minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) of A. ursinum extract for six bacterial pathogens were determined using the broth macrodilution method. Although the A. ursinum extract was shown to be effective toward all investigated foodborne ba ...
... Studies on trimethylamine (TMA) in egg yolk have focused on how it impacts the flavor of eggs, but there has been little focus on its other functions. We designed an in vitro antibacterial test of TMA according to TMA concentrations that covered the TMA contents typically found in egg yolk. The change in TMA content in yolk was analyzed at different storage temperatures and for different storage d ...
... The use of contaminated water by bacteria may cause many diseases, and thus clean water is needed. Chlorine is normally used for the disinfection of wastewater treatment; however, it produces unwanted odors. Using extracted Piper betle (P. betle) is an interesting choice because it is a good chemical compound for bacterial inhibitions. This study attempted to extract P. betle leaf and synthesize P ...
... The antibacterial activity of methanolic extracts of eight Mediterranean brown algae belonging to the genera Cystoseira sensu lato, Dictyopteris and Padina was investigated. Extracts from Sargassaceae showed antibacterial activity. Specifically, Carpodesmia crinita and Carpodesmia brachycarpa extracts inhibited the growth of the Gram-positive strain Kocuria rhizophila whereas Cystoseira compressa ...
... To evaluate the antibacterial effect of Khadirarishta (An Ayurvedic traditional formulation) against some of the bacterial strains as well as to investigate the probable mechanism of action against sensitive bacterial strains by using standard procedures. The antibacterial activity of Khadirarishta against some of the bacterial strains was evaluated by using bacterial susceptibility assay and the ...
... In this paper, we describe the green synthesis of silver nanoparticles (AgNPs) using plant extracts, as well as their structural, optical, and antibacterial activities against Escherichia coli and Bacillus velezensis bacteria. For the study, several sections (root, leaves, and seed) of Mucuna pruriens, an immense medicinal herbal used to treat Parkinson's disease, were evaluated. Also, the seeds w ...
... We studied a strain of Bacillus isolated from an artisanal tannery in Salta, Argentina. It was identified as Bacillus licheniformis B6 by 16 S phylogenetic analysis and MALDI TOF (GenBank accession code No. KP776730). The synthesis of lipopeptides by B6 and their antibacterial activity against clinical pathogenic strains was analyzed both in the cell-free supernatant (CFS) and in the crude fractio ...
... Pork and its products are preferably contaminated by bacteria; thus, it is essential to develop low-cost, high-efficiency and biologically safe preservatives to prevent the growth of bacteria during storage. In the current study, grass carp β-defensin 1 (gcDefb1) was produced and purified from Pichia pastoris through the heterologous expression method. The in vitro antimicrobial assay demonstrated ...
Escherichia coli; Proteus vulgaris; antibacterial properties; cell death; chemical reduction; copper nanoparticles; growth retardation; hydrodynamics; membrane permeability; minimum inhibitory concentration; multiple drug resistance; oxidative stress; reactive oxygen species
Abstract:
... Antibacterial activities of as‐synthesized nanoparticles have gained attention in past few years due to rapid phylogenesis of pathogens developing multi‐drug resistance (MDR). Antibacterial activity of copper nanoparticles (CuNPs) on surrogate pathogenic Gram‐negative bacteria Escherichia coli (MTCC no. 739) and Proteus vulgaris (MTCC no. 426) was evaluated under culture conditions. Three sets of ...
... Among the different metal oxide nanoparticles, zinc oxide nanoparticles have gained significant importance due to their antibacterial properties against clinically pathogenic bacteria during the organal development. In the present study, biogenic zinc oxide nanoparticles were synthesized using seed extract of Citrus limon by a simple, cost-effective, and green chemistry approach. The synthesized Z ...
... Nanoencapsulation has been verified to be an effective technique to improve the physical stability of essential oils. In this study, Ocimum basilicum L. essential oil (BEO) was encapsulated into chitosan nanoparticles by emulsion and ionic gelation. The success of BEO loading was revealed by Fourier transform infrared (FTIR) spectroscopy, ultraviolet visible spectrophotometry and X-ray diffraction ...
... Chinese water chestnut (Eleocharis tuberosa) is prone to etiolation after fresh-cut treatment. Though etiolation caused undesired yellow color, it comes with accumulation of bioactive flavonoids. In this work, flavonoids were extracted from etiolated fresh-cut Chinese water chestnut. Their species and content were investigated. The antibacterial and hypoglycemic activities of crude extract were al ...
... Quaternary ammonium compounds (QACs) have outstanding antimicrobial effect, but covalent immobilization of plentiful QAC onto cotton fiber surface to realize a durable function remains a challenge. Herein, a quaternary ammonium monomer, [2-(methacryloyloxy) ethyl] trimethylammonium chloride (DMC) was co-polymerized with methyl acrylate (MA) to prepare an antibacterial copolymer, poly(DMC-co-MA). T ...
... The objectives of this study were (1) to prepare Armillariella tabescens mycelia polysaccharides (PAT) with remarkably growth inhibitory effect on typical food-borne pathogenic bacteria using a green and efficient polyamide method and (2) to explore the antibacterial mechanism of PAT for use as a natural antibacterial agent. The sugar and uronic acid contents of PAT were 93.41% and 12.24%, respect ...
... Lindera aggregata (Sims) Kosterm. is a traditional Chinese herb, which has been proven to have excellent antibacterial activity. In this work, we firstly extracted the polysaccharides from the leaves of Lindera aggregata (Sims) Kosterm. (LLPs), and explored their antibacterial activity and related mechanisms. The experimental results show that LLPs are a good antibacterial agent, which can damage ...
... In the study, leaf extract of Carica papaya was utilized for the biogenic fabrication process of chitosan functionalized silver nanoparticles (Ag-Chito NPs). HRTEM analysis revealed that the fabricated Ag-Chito NPs was spherical in shape, with an average particle size of 13.31 (±0.07) nm. FTIR, UV–Vis, DLS, and other characterizations were also performed to analyze the diverse physicochemical prop ...
... Ferulic (F) and cinnamic (C) acids have shown great potential as antimicrobial additives in the development of active food packaging based on biodegradable polymers. However, previous studies on thermo-processed (TP) PLA matrices with 1 or 2%w/w of these compounds found no microbial growth inhibition due to their limited release. In this study, 2 or 3% of F and C were incorporated into starch and ...
... Biosynthesis of silver nanoparticles (AgNP) using plant extracts is considered as an advanced method of synthesising AgNP using the green method. Although the leaf and the root of Ficus deltoidea Jack var. kunstleri (King) Corner (Mas Cotek) are known to have medicinal benefits, the study on its organs (leaf, stem, fig and root) to synthesise AgNP is still scarce. Therefore, this study determined ...
... The auraptene is a geranyloxyn coumarin found in the Ferula species. The plant is endemic in Central Asia and it is used as a medicinal food in Iran. This research aimed to evaluate the antibacterial, antioxidant, and anti-melanogenic properties of auraptene, a coumarin from Ferula szowitsiana root. The results revealed that auraptene possessed antibacterial activity with minimum inhibitory and mi ...
Escherichia coli; Salmonella Enteritidis; aminoglycosides; antibiotic resistance; beta-lactamase bacteria; cattle; ceftiofur; chickens; genes; plasmids; public health; swine; tetracycline; Korean Peninsula
Abstract:
... Extended-spectrum β-lactamase (ESBL)-producing Salmonella enterica serovar Enteritidis has emerged as a public health concern. The main objectives of this study were therefore to determine the antimicrobial susceptibility profiles of Salmonella Enteritidis and to investigate the molecular characteristics of identified ESBL-producing isolates. In the study, 237 Salmonella Enteritidis isolates (232 ...
... Although its importance, little information is available on antibiotic-resistance in cow-calf beef farms. This study aimed to determine prevalence and risk factors for antibiotic-resistant organisms in this livestock system. Fifty-four farms from Central Italy were included to assess the presence of antibiotic-resistant indicator Escherichia coli and of ESBL and/or AmpC-producing E. coli (ESBL/Amp ...
... Escherichia coli is considered the main cause of intestinal and extra-intestinal infections, they have virulence mechanisms in different pathotypes and the ability to receive or transmit antimicrobial resistance genes. The aim of this work was to investigate the antibacterial and antimicrobial modulating activity of α-pinene and borneol against E. coli and enteropathogenic (EPEC) and enterotoxigen ...