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You searched for: Subject term G-protein coupled receptors Remove constraint Subject term: G-protein coupled receptors Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. Evidence for hydrophobic interaction between galanin and the Ga1R1 galanin receptor and Ga1R1-mediated ligand internalization: fluorescent probing with a fluorescein-galanin

Author:
Wang, S.; Clemmons, A.; Strader, C.; Bayne, M.
Source:
Biochemistry 1998 v.37 no.26 pp. 9528-9535
ISSN:
0006-2960
Subject:
Chinese hamsters; G-protein coupled receptors; animal ovaries; binding sites; flow cytometry; fluorescein; fluorescence; hydrophilicity; hydrophobic bonding; hydrophobicity; ligands; neuropeptides; plasma membrane; rats; sucrose
Abstract:
... Galanin is a neuropeptide that activates specific receptors to modulate several physiological functions including food intake, nociception, and learning and memory. The molecular nature of the interaction between galanin and its receptors and the fate of the galanin/receptor complex after the binding event are not understood. A fluorescein-N-galanin (F-Gal) was generated to measure the interaction ...
DOI:
10.1021/bi9731955

2. Location of mouse and human genes corresponding to conserved canine olfactory receptor gene subfamilies

Author:
Ethan A. Carver; Laurie Issel-Tarver; Jasper Rine; Anne S. Olsen; Lisa Stubbs
Source:
Mammalian genome 1998 v.9 no.5 pp. 349-354
ISSN:
0938-8990
Subject:
G-protein coupled receptors; chromosomes; dogs; epithelium; humans; mice; multigene family; nose; odor compounds; olfactory receptors
Abstract:
... Olfactory receptors are G protein-coupled, seven-transmembrane-domain proteins that are responsible for binding odorants in the nasal epithelium. They are encoded by a large gene family, members of which are organized in several clusters scattered throughout the genomes of mammalian species. Here we describe the mapping of mouse sequences corresponding to four conserved olfactory receptor genes, e ...
DOI:
10.1007/s003359900768

3. Differential stimulation of signaling pathways initiated by Edg-2 in response to lysophosphatidic acid or sphingosine-1-phosphate

Author:
O. Peyruchaud*, **; D. F. Mosher
Source:
Cellular and molecular life sciences 2000 v.57 no.7 pp. 1109-1116
ISSN:
1420-682X
Subject:
G-protein coupled receptors; actin; fibronectins; humans; lipids; osteosarcoma; signal transduction
Abstract:
... Lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are produced during cell activation and have multiple effects on cells. A family of seven transmembrane-spanning domain G-protein-coupled receptors, named Edg, mediate these effects of LPA and S1P. In this study, transient overexpression of Edg-2 sensitized MG63 human osteosarcoma cells to both LPA- and S1P-mediated stimulation of fibro ...
DOI:
10.1007/PL00000747

4. Evidence against a direct role of the integrin α2β1 in collagen‐induced tyrosine phosphorylation in human platelets

Author:
Ingeborg Hers; Oscar Berlanga; Margriet J. Tiekstra; Aura S. Kamiguti; R. David G. Theakston; Steve P. Watson
Source:
European journal of biochemistry 2000 v.267 no.7 pp. 2088-2097
ISSN:
0014-2956
Subject:
G-protein coupled receptors; adhesion; agonists; antibodies; collagen; crosslinking; glycoproteins; humans; integrins; metalloproteinases; phosphorylation; phosphotransferases (kinases); platelet activation; snakes; thrombin; tyrosine; venoms
Abstract:
... In the present study we have investigated whether the collagen receptor α2β1 (GPIa‐IIa; GP, glycoprotein) regulates protein tyrosine phosphorylation in platelets directly through activation of tyrosine kinases or indirectly through modification of the response to GPVI. The interaction of collagen with α2β1 was inhibited in two distinct ways, using the metalloprotease jararhagin, which cleave ...
DOI:
10.1046/j.1432-1327.2000.01214.x

5. Expression and characterization of Edg‐1 receptors in rat cardiomyocytes: Calcium deregulation in response to sphingosine 1‐phosphate

Author:
Nobuko Nakajima; Amy L. Cavalli; Donatella Biral; Christopher C. Glembotski; Patrick M. McDonough; Peter D. Ho; Romeo Betto; Doriana Sandoná; Philip T. Palade; Christine A. Dettbarn; Robert E. Klepper; Roger A. Sabbadini
Source:
European journal of biochemistry 2000 v.267 no.18 pp. 5679-5686
ISSN:
0014-2956
Subject:
protein synthesis; sphingosine; complementary DNA; myocardial ischemia; calcium; G-protein coupled receptors; blood platelets; cardiomyocytes; rats; sphingolipids
Abstract:
... Recent evidence indicates that sphingolipids are produced by the heart during hypoxic stress and by blood platelets during thrombus formation. It is therefore possible that sphingolipids may influence heart cell function by interacting with G‐protein‐coupled receptors of the Edg family. In the present study, it was found that sphingosine 1‐phosphate (Sph1P), the prototypical ligand for Edg r ...
DOI:
10.1046/j.1432-1327.2000.01656.x

6. Expression patterns of the regulatory proteins G protein‐coupled receptor kinase 2 and β‐arrestin 1 during rat postnatal brain development: Effect of hypothyroidism

Author:
Petronila Penela; Manuel Álvarez‐Dolado; Alberto Muñoz; Federico Mayor Jr
Source:
European journal of biochemistry 2000 v.267 no.14 pp. 4390-4396
ISSN:
0014-2956
Subject:
G-protein coupled receptors; adults; cerebral cortex; hypothyroidism; messenger RNA; rats; regulatory proteins
Abstract:
... G protein‐coupled receptor kinase 2 (GRK2) and β‐arrestin 1 are key regulatory proteins that modulate the desensitization and resensitization of a wide variety of G protein‐coupled receptors (GPCRs) involved in brain functions. In this report, we describe the postnatal developmental profile of the mRNA and protein levels of GRK2 and β‐arrestin 1 in rat brain. The expression levels of GRK2 and β‐ar ...
DOI:
10.1046/j.1432-1327.2000.01484.x

7. Human oxytocin receptors in cholesterol‐rich vs. cholesterol‐poor microdomains of the plasma membrane

Author:
Gerald Gimpl; Falk Fahrenholz
Source:
European journal of biochemistry 2000 v.267 no.9 pp. 2483-2497
ISSN:
0014-2956
Subject:
G-protein coupled receptors; agonists; cholesterol; fractionation; functional properties; green fluorescent protein; guanosine triphosphate; humans; oxytocin receptors; plasma membrane; solubilization
Abstract:
... We analyzed the properties of a G protein‐coupled receptor localized in cholesterol‐poor vs. cholesterol‐rich microdomains of the plasma membrane. For this purpose, the human oxytocin receptor, which is very sensitive against alterations of the membrane cholesterol level, was stably expressed in HEK293 cells. To calculate the total number of receptors independent of ligand binding studies, the oxy ...
DOI:
10.1046/j.1432-1327.2000.01280.x

8. Monitoring of the internalization of neuropeptide Y on neuroblastoma cell line SK‐N‐MC

Author:
Marlies Fabry; Michael Langer; Barbara Rothen‐Rutishauser; Heidi Wunderli‐Allenspach; Hartwig Höcker; Annette G. Beck‐Sickinger
Source:
European journal of biochemistry 2000 v.267 no.17 pp. 5631-5637
ISSN:
0014-2956
Subject:
G-protein coupled receptors; acid tolerance; agonists; confocal laser scanning microscopy; endocytosis; fluorescence; humans; monitoring; neuropeptide Y; peripheral nervous system; washing
Abstract:
... Neuropeptide Y (NPY) is an important neuromodulator in the central and peripheral nervous system. The peptide acts through different NPY receptor subtypes (Y1–Y5, y6) that belong to the family of G protein‐coupled receptors. In general, cellular responses to prolonged exposure to agonists of G protein‐coupled receptors are attenuated, often through internalization of the receptors and ...
DOI:
10.1046/j.1432-1327.2000.01631.x

9. Regulation of receptor function by cholesterol

Author:
K. Burger; G. Gimpl; F. Fahrenholz
Source:
Cellular and molecular life sciences 2000 v.57 no.11 pp. 1577-1592
ISSN:
1420-682X
Subject:
G-protein coupled receptors; binding capacity; cholesterol; cholinergic receptors; organelles; signal transduction
Abstract:
... Cholesterol influences many of the biophysical properties of membranes and is nonrandomly distributed between cellular organelles, subdomains of membranes, and leaflets of the membrane bilayer. In combination with the high dynamics of cholesterol distribution, this offers many possibilities for regulation of membrane-embedded receptors. Depending on the receptor, cholesterol can have a strong infl ...
DOI:
10.1007/PL00000643

10. Coupling of endothelin receptors to the ERK/MAP kinase pathway : Roles of palmitoylation and Gαq

Author:
Henning Cramer; Kai Schmenger; Khristina Heinrich; Angelika Horstmeyer; Hilke Böning; Andreas Breit; Albrecht Piiper; Kenneth Lundstrom; Werner Müller‐Esterl; Christian Schroeder
Source:
European journal of biochemistry 2001 v.268 no.20 pp. 5449-5459
ISSN:
0014-2956
Subject:
G-protein coupled receptors; acylation; binding capacity; binding sites; cAMP-dependent protein kinase; calcium; endothelins; epidermal growth factor receptors; mitogen-activated protein kinase; mutants; palmitoylation; phosphorylation; protein binding; tyrosine
Abstract:
... Endothelins are potent mitogens that stimulate extracellular signal‐regulated kinases (ERK/MAP kinases) through their cognate G‐protein‐coupled receptors, ETA and ETB. To address the role of post‐translational ET receptor modifications such as acylation on ERK activation and to identify relevant downstream effectors coupling the ET receptor to the ERK signaling cascades we have constructed ...
DOI:
10.1046/j.0014-2956.2001.02486.x

11. Differential regulation of cAMP‐mediated gene transcription and ligand selectivity by MC3R and MC4R melanocortin receptors

Author:
Eun Jin Lee; Soo‐Hyun Lee; Jin‐Won Jung; Weontae Lee; Byung Jin Kim; Kye Won Park; Sung‐Kil Lim; Chang‐Ju Yoon; Ja‐Hyun Baik
Source:
European journal of biochemistry 2001 v.268 no.3 pp. 582-591
ISSN:
0014-2956
Subject:
human cell lines; nuclear magnetic resonance spectroscopy; G-protein coupled receptors; luciferase; models; transcription (genetics); gene expression; adenylate cyclase; reporter genes; alpha-melanocyte-stimulating hormone
Abstract:
... Melanocortins are known to be involved in the regulation of feeding behavior. These hormones mediate their effects through G‐protein‐coupled receptors by stimulating adenylate cyclase. In this study we describe the functional response of melanocortin 4 receptor (MC4R) and melanocortin 3 receptor (MC3R) in HEK 293T cells, by using a luciferase reporter gene under the transcriptional control of a cA ...
DOI:
10.1046/j.1432-1327.2001.01900.x

12. G-Protein-Mediated Signaling and Its Control in Macrophages and Mammalian Cells

Author:
Prasanta Chakraborty
Source:
Critical reviews in microbiology 2001 v.27 no.1 pp. 1-8
ISSN:
1549-7828
Subject:
G-protein coupled receptors; G-proteins; GTPase-activating proteins; agonists; cell proliferation; crosslinking; gene expression; ligands; macrophages; mammals; mitogen-activated protein kinase; pathogens; signal transduction
Abstract:
... G-protein coupled receptor (GPCR) in various cell types exert its effects through heterotrimetic GTP-binding proteins (G-proteins). The interaction of specific ligand or agonists with CPCR transuces signal and enhances gene expression, mitogen activated protein kinase (MAP kinase) activation, and thus regulates cell proliferation, differentation, and motility. Abnormal signaling or prolonged activ ...
DOI:
10.1080/20014091096666

13. Neurotensin induces mating in Saccharomyces cerevisiae cells that express human neurotensin receptor type 1 in place of the endogenous pheromone receptor

Author:
Pascal Leplatois; Annick Josse; Marie Guillemot; Martine Febvre; Natalio Vita; Pascual Ferrara; Gérard Loison
Source:
European journal of biochemistry 2001 v.268 no.18 pp. 4860-4867
ISSN:
0014-2956
Subject:
G-protein coupled receptors; Saccharomyces cerevisiae; antagonists; beta-galactosidase; binding sites; diploidy; dose response; genes; glycosylation; haploidy; humans; molecular weight; pheromones; polypeptides; yeasts; zygote
Abstract:
... Heterologous expression of the human neurotensin receptor type I (hNT1‐R) has been achieved in the yeast Saccharomyces cerevisiae. Immunoanalysis of membranes prepared from cells expressing a c‐myc‐tagged version of hNT1‐R revealed multiple c‐myc cross‐reacting polypeptides of high molecular mass, suggesting that hNT1‐R was glycosylated in yeast. High‐affinity binding sites for 125I‐labeled‐[monoi ...
DOI:
10.1046/j.0014-2956.2001.02407.x

14. Palmitoylation of the luteinizing hormone/human chorionic gonadotropin receptor regulates receptor interaction with the arrestin‐mediated internalization pathway

Author:
Utpal M. Munshi; Helle Peegel; K. M. J. Menon
Source:
European journal of biochemistry 2001 v.268 no.6 pp. 1631-1639
ISSN:
0014-2956
Subject:
G-protein coupled receptors; cysteine; gene overexpression; gonadotropin receptors; human chorionic gonadotropin; inositol phosphates; luteinizing hormone; mutants; palmitoylation
Abstract:
... The luteinizing hormone/human chorionic gonadotropin receptor (LH/hCGR) undergoes palmitoylation at cysteine residues 621 and 622 located in the carboxyl terminal tail of the receptor. This study examined the biological function of palmitoylation with respect to its effect on receptor internalization. Coexpression of wild‐type (WT) or C621/622G mutant receptors with arrestin‐2 increased receptor i ...
DOI:
10.1046/j.1432-1327.2001.02032.x

15. Source and component genes of a 6–200 Mb gene cluster in the house mouse

Author:
Dieter Weichenhan; Bärbel Kunze; Heinz Winking; Michel van Geel; Kazutoyo Osoegawa; Pieter J. de Jong; Walther Traut
Source:
Mammalian genome 2001 v.12 no.8 pp. 590-594
ISSN:
0938-8990
Subject:
G-protein coupled receptors; Mus musculus; chimerism; gene fusion; genes; transcription (genetics)
Abstract:
... We identified and analyzed the genes Sp100, Csprs, and Ifi75 in two members of the genus Mus, M. musculus and M. caroli. Sp100 is a nuclear dot gene; Csprs and Ifi75 are novel genes encoding a putative G-protein coupled receptor (GPCR) and a putative transcriptional coactivator, respectively. A fourth gene, Sp100-rs, occurs in M. musculus, but not in M. caroli. Sp100-rs is a chimeric gene which ar ...
DOI:
10.1007/s00335-001-3015-9
PubMed:
11471051

16. The role of hexose transport and phosphorylation in cAMP signalling in the yeast Saccharomyces cerevisiae

Author:
Rolland, Filip; Wanke, Valeria; Cauwenberg, Liesbet; Ma, Pingsheng; Boles, Eckhard; Vanoni, Marco; de Winde, Johannes H.; Thevelein, Johan M.; Winderickx, Joris
Source:
FEMS yeast research 2001 v.1 no.1 pp. 33-45
ISSN:
1567-1356
Subject:
G-protein coupled receptors; Saccharomyces cerevisiae; acidification; adenosine triphosphate; cyclic AMP; glucose; hydrolysis; maltose; metabolism; phosphorylation; phosphotransferases (kinases); yeasts
Abstract:
... Glucose-induced cAMP signalling in Saccharomyces cerevisiae requires extracellular glucose detection via the Gpr1-Gpa2 G-protein coupled receptor system and intracellular glucose-sensing that depends on glucose uptake and phosphorylation. The glucose uptake requirement can be fulfilled by any glucose carrier including the Gal2 permease or by intracellular hydrolysis of maltose. Hence, the glucose ...
DOI:
10.1111/j.1567-1364.2001.tb00011.x

17. Ascidian arrestin (Ci‐arr), the origin of the visual and nonvisual arrestins of vertebrate

Author:
Masashi Nakagawa; Hidefumi Orii; Norihiro Yoshida; Eri Jojima; Takeo Horie; Reiko Yoshida; Tatsuya Haga; Motoyuki Tsuda
Source:
European journal of biochemistry 2002 v.269 no.21 pp. 5112-5118
ISSN:
0014-2956
Subject:
Chordata; G-protein coupled receptors; amino acid sequences; arrestins; genes; larvae; phosphotransferases (kinases); photoreceptors; phylogeny; retina; signal transduction; vertebrates
Abstract:
... Arrestin is one of the key proteins for the termination of G protein signaling. Activated G protein‐coupled receptors (GPCRs) are specifically phosphorylated by G protein‐coupled receptor kinases (GRKs) and then bind to arrestins to preclude the receptor/G protein interaction, resulting in quenching of the following signal transduction. Vertebrates possess two types of arrestin; visual arresti ...
DOI:
10.1046/j.1432-1033.2002.03240.x

18. Probing intermolecular protein–protein interactions in the calcium‐sensing receptor homodimer using bioluminescence resonance energy transfer (BRET)

Author:
Anders A. Jensen; Jakob L. Hansen; Søren P. Sheikh; Hans Bräuner‐Osborne
Source:
European journal of biochemistry 2002 v.269 no.20 pp. 5076-5087
ISSN:
0014-2956
Subject:
G-protein coupled receptors; G-proteins; agonists; bioluminescence; energy transfer; green fluorescent protein; luciferase; mutants; protein-protein interactions
Abstract:
... The calcium‐sensing receptor (CaR) belongs to family C of the G‐protein coupled receptor superfamily. The receptor is believed to exist as a homodimer due to covalent and non‐covalent interactions between the two amino terminal domains (ATDs). It is well established that agonist binding to family C receptors takes place at the ATD and that this causes the ATD dimer to twist. However, very li ...
DOI:
10.1046/j.1432-1033.2002.03218.x

19. Progestin upregulates G‐protein‐coupled receptor 30 in breast cancer cells

Author:
Tytti M. Ahola; Sami Purmonen; Pasi Pennanen; Ya‐Hua Zhuang; Pentti Tuohimaa; Timo Ylikomi
Source:
European journal of biochemistry 2002 v.269 no.10 pp. 2485-2490
ISSN:
0014-2956
Subject:
G-protein coupled receptors; acetates; breast neoplasms; dexamethasone; dose response; gene expression; genes; growth retardation; medroxyprogesterone; messenger RNA; mitogen-activated protein kinase; neoplasm cells; progesterone
Abstract:
... A differential display method was used to study genes the expression of which is altered during growth inhibition induced by medroxyprogesterone acetate (MPA). A transcript of G‐protein‐coupled receptor 30 (GPR30) was upregulated by MPA in estrogen‐treated MCF‐7 breast cancer cells. Northern‐blot analysis showed a progestin‐specific primary target gene, which was enhanced by progestero ...
DOI:
10.1046/j.1432-1033.2002.02912.x

20. Proteolytic action of duodenase is required to induce DNA synthesis in pulmonary artery fibroblasts: A role for phosphoinositide 3‐kinase

Author:
Alan D. Pemberton; Tatyana S. Zamolodchikova; Cheryl L. Scudamore; Edwin R. Chilvers; Hugh R. P. Miller; Trevor R. Walker
Source:
European journal of biochemistry 2002 v.269 no.4 pp. 1171-1180
ISSN:
0014-2956
Subject:
phosphatidylinositol 3-kinase; serine; substrate specificity; trypsin inhibitors; DNA; G-protein coupled receptors; inflammation; proteolysis; pulmonary artery; pertussis toxin; protein kinase C; soybeans; thymidine; intestinal mucosa; mast cells; tumor necrosis factor-alpha; fibroblasts; interleukin-1beta
Abstract:
... Duodenase is a 29‐kDa serine endopeptidase that displays selective trypsin‐ and chymotrypsin‐like substrate specificity. This enzyme has been localized to epitheliocytes of Brunner's glands, and as described here, to mast cells within the intestinal mucosa and lungworm‐infected lung, implying an important additional role in inflammation and tissue remodelling. In primary cultures of pulmon ...
DOI:
10.1046/j.1432-1033.2002.02747.x