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... A thermostable cyclodextrin glucanotransferase (CGTase) was isolated from a Bacillus stearothermophilus strain, ET1, which was screened from Korean soil. The corresponding CGTase gene cloned in Escherichia coli shared 84% and 88% identity with CGTase genes from other B. stearothermophilus strains at the nucleotide and amino acid sequence level, respectively. The enzyme was purified to apparent hom ...
dietary fiber; in vitro digestibility; corn starch; GRAS substances; bulking agents; maltodextrins; blood glucose; liveweight gain; intestinal absorption; rats; metabolizable energy; excretion; United States; Japan
Abstract:
... A series of in vivo and in vitro experiments was conducted to determine the available energy of a bulking agent, Fibersol 2 (FS-2). Fibersol 2 is obtained by a combination of heat and enzyme treatments of cornstarch to produce a low-viscosity, low-digestible dextrin with an average molecular massweight of 2000 Da. Although the substance is of the type known in Japan as an indigestible dextrin, it ...
... A new method for the selection of transgenic plants has been developed. It is based upon selection of transgenic plant cells expressing the xylA gene from Streptomyces rubiginosus, which encodes xylose isomerase, on medium containing xylose. The xylose isomerase selection system was tested in potato and the transformation frequency was found to be approximately ten fold higher than with kanamycin ...
... The objective of this study was to evaluate the effects of soy protein isolate (SPI), three forms of native corn gluten meal on sensory, color and textural characteristics of an emulsified meat product. The meals included native corn gluten meal (CGM) that had been ph-adjusted from 4.4 to 6.6 and particle size reduced to either ~38 (MC38) or ~7 micrometer (MC7). When judged by a trained sensory pa ...
... A method for the determination of six kava lactones, methysticin, dihydromethysticin, kawain, dihydrokawain, yangonin and desmethoxyyangonin, in solid foods and beverages has been developed. Solid samples were prepared using methanol extraction, while beverages were extracted using a separate solid phase extraction (SPE) method. After sample preparation, the extracts were analysed using LC-UV or a ...
... Antimicrobial additives have been used successfully for many years as direct food additives. The literature provides evidence that some of these additives may be effective as indirect food additives incorporated into food packaging materials. Antimicrobial food packaging is directed toward the reduction of surface contamination of processed, prepared foods such as sliced meats and Frankfurter saus ...
GRAS substances; food preservatives; antioxidants; butylated hydroxyanisole; butylated hydroxytoluene; dietary nutrient sources; dietary surveys; nutrient intake; estimation; maximum permissible intake; South Korea
Abstract:
... The study was conducted to establish the estimated daily intake (EDI) of antioxidants such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and tert-butyl hydroquinone (TBHQ) in Korea. The EDIs were obtained from two sources. One of the estimations was based on the analytical determination of BHA, BHT and TBHQ in 12 food categories (ten food categories for TBHQ) and on individual ...
laws and regulations; Food and Agriculture Organization; product safety; packaging materials; foil; GRAS substances; heavy metals; toxic substances; food packaging; aluminum; World Health Organization
Abstract:
... The use of silver foils in various food preparations is a common practice in Middle Eastern and South East Asian countries. The FAO/WHO Joint Expert Committee on Food Additives (JECFA) has included silver in the list of food additives, but specifications were not prepared. Indian food legislation has included food-grade silver foil and laid down a purity requirement of 99.9%. This leaves an unspec ...
food analysis; quantitative analysis; copper; GRAS substances; chemical analysis; food coloring; reversed-phase high performance liquid chromatography; chlorophyll; beverages; food retailing
Abstract:
... An analytical method using high performance liquid chromatography with photodiode array and fluorescence detection has been developed and applied to the determination of the food colour additives copper chlorophylls and copper chlorophyllins (E141i and ii) in foods and beverages. The analytical procedures from previously reported methods have been refined to cover a range of food colour formulatio ...
... Members of the GRAS family of transcriptional regulators have been implicated in the control of plant growth and development, and in the interaction of plants with symbiotic bacteria. Here we examine the complexity of the GRAS gene family in tomato (Solanum lycopersicum) and investigate its role in disease resistance and mechanical stress. A large number of tomato ESTs corresponding to GRAS transc ...
flavorings; GRAS substances; product safety; safety testing; maximum permissible intake; estimation; Food and Agriculture Organization; World Health Organization
... In the present study, soybean koji fermented with various GRAS filamentous fungi, including Aspergillus sojae BCRC 30103, Aspergillus oryzae BCRC 30222, Aspergillus awamori, Actinomucor taiwanensis and Rhizopus sp. These organisms are commonly used as starters in the fermentation of many traditional, oriental food products. The growth of starter organisms, total phenolic content, and antioxidative ...
... To determine the effect of a composition comprising ovotransferrin (OT), protamine sulfate (PS) and ethylenediaminetetraacetic acid (EDTA) on biofilm formation by catheter-associated bacteria. The in vitro activity of OT, PS and EDTA alone and in combinations against biofilm formation by Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Enterococcus faecalis and S ...
... Enterococcus faecalis produces a cationic and circular enterocin, AS-48, of 7149 Da, the genetic determinants of which are located within the pMB2 plasmid. We have compared enterocin AS-48 production by different enterococci species with that of other 'safe' lactic acid bacteris (LAB) (GRAS status) and looked into the subsequent application of this enterocin in food production. In an effort to exp ...
... The ochratoxin A (OTA) content of 30 samples of licorice root and derived products (licorice-confectionery, licorice block, and licorice extract) was analyzed by a standard HPLC-fluorescence technique and confirmed by methyl-ester formation. All analyzed samples of licorice and derived products were found to contain ochratoxin A, and some of them showed extremely high concentrations up to 252.8 ng ...
... Microbial hyaluronic acid (HA), commonly produced by pathogenic Streptococcus, was made possible to be produced by a generally recognized as safe Lactococcus lactis by coexpressing HA synthase and uridine diphosphate–glucose dehydrogenase (UDP-GlcDH) of Streptococcus equi subsp. zooepidemicus in a nisin-controlled expression (NICE) system. With scarce expressed HA synthase alone, the constructed r ...
GRAS substances; cheeses; cluster analysis; dairy industry; ecology; lactic acid bacteria; random amplified polymorphic DNA technique; raw milk; ribosomal RNA; sensory properties; sequence analysis; starter cultures; Italy
Abstract:
... Identification and biotyping of lactic acid bacteria (LAB) isolated from raw-milk Pecorino cheese manufactured in the Marche region (central Italy) for selection of suitable starter cultures or adjuncts. Preliminary characterization with morphological and biochemical assays were undertaken for 112 Gram-positive and catalase-negative isolates. Unequivocal identification of the isolates was obtained ...
... The potential for natural antimicrobial compounds extracted from true cinnamon (Cinnamomum zeylanicum Nees ) to use as a food additive to extend the shelf life of fresh-cut apples was investigated. Several different extracts were prepared using cinnamon bark and powder to evaluate their antimicrobial activity on two marker microorganisms, Escherichia coli O157:H7 and Listeria innocua. An ethanolic ...
... A comprehensive study on thymol loaded zein films is presented in this work. In particular, the antimicrobial efficacy of developed active zein films was tested against three different spoilage microorganisms (cells and spores of Bacillus cereus, Candida lusitaniae and Pseudomonas spp.) and one GRAS (Generally recognized as safe) microbial strain (Streptococcus thermophilus). Results show that the ...
Food and Drug Administration; GRAS substances; aqueous solutions; atomization; carbon dioxide; droplets; ethanol; food composition; food quality; foods; ingredients; isopropyl alcohol; mass transfer; methanol; nanoparticles; polymers; solvents; zein
Abstract:
... To develop edible delivery systems suitable for food applications, regulations require that solvents and ingredients are either generally recognized as safe or listed by the Food and Drug Administration as processing aids. In this work, we studied a food grade polymer-corn zein, a category of alcohol-soluble proteins, as the carrier material for microencapsulating bioactives. Zein is insoluble in ...
... Allura Red-40 is a safe colour additive (permissible by the FDA and Health Canada) that is used in a variety of foods to make them more attractive and appealing for consumers. However, limited information is available about its binding to macronutrients that are responsible for its uniform distribution in food products. In the present study, the binding capacity of Allura with food proteins is com ...
... Contamination of ready-to-eat (RTE) meat products such as ham steaks with Listeria monocytogenes has been a concern for the meat processing industry. The objective of this study was to evaluate the antilisterial efficacy of chitosan-coated plastic films alone or incorporating five generally recognized as safe (GRAS) antimicrobials. Effect of chitosan-coated plastic film on the growth of L. monocyt ...
... The objective of this study was to evaluate the efficacy of chitosan-coated plastic films incorporating five Generally Recognized as Safe (GRAS) antimicrobials (nisin, sodium lactate (SL), sodium diacetate (SD), potassium sorbate (PS) and sodium benzoate (SB)) against Listeria monocytogenes on cold-smoked salmon. Salmon samples were surface-inoculated with a five-strain cocktail of L. monocytogene ...
... The use of novel nanostructured materials has attracted considerable interest in the food industry for their utilization as highly functional ingredients, high-performance packaging materials, processing aids, and food quality and safety sensors. Most previous application interest has focused on the development of nanoparticles. However, more recently, the ability to produce non-woven mats compose ...
... To assure the homogeneity of a reference material for the mycotoxin sterigmatocystin (STE), a study was first conducted to prepare a reference material of rice containing the colouring Food Red 106. The protocol developed was then used to prepare a reference material of rice containing STE. Initially, a V-shaped mixer was used to mix Food Red 106 and ground brown rice, but the resulting mixture wa ...
genome; dairies; propionic acid bacteria; hard cheeses; multiple drug resistance; Bifidobacterium; virulence; dairy industry; cheese starters; anaerobes; cheese ripening; fermented milk; food safety; food biopreservatives; human health and safety; antibiotic resistance; GRAS substances; lactic acid bacteria; probiotics
Abstract:
... The genera Propionibacterium and Bifidobacterium are clustered in the class Actinobacteria and form the anaerobic branch of coryneform bacteria. The dairy propionibacteria comprising four species P. freudenreichii, P. acidipropionici, P. jensenii and P. thoenii are industrially important as starter cultures in hard-cheese ripening and recently also as protective bio-preservatives and probiotics. T ...
... Unlike most of lactic acid bacteria, the Enterococcus genus is not considered “generally recognized as safe” (GRAS). Safety assessment for enterococci remains controversial. While enterococci are considered “positive" or useful in cheese technology, isolates of this genus have emerged as opportunistic pathogens for humans. Thus these bacteria have the paradoxical position of being useful in dairy ...
... The Lactococcus genus includes 5 species. Lactococcus lactis subsp. lactis is the most common in dairy product but L. garviae has been also isolated. Their biotope is animal skin and plants. Owing to its biochemical characteristics, strains of L. lactis are widely used in dairy fermented products processing. Cases of human infections due to lactococci are very seldom reported even if Lactococcus g ...
food spoilage; human health and safety; phenotype; GRAS substances; biogenic amines; chemotaxonomy; risk factors; Leuconostoc; probiotics; dairies; fermented milk; cultured milk starters; bacterial infections; food safety
Abstract:
... Although Leuconostoc genus is “generally recognized as safe” (GRAS), a few clinically human infections cases by this microorganism have been reported in the literature, leading to their classification as opportunistic pathogens. However, these reported cases concern only severe immunodepressed patients, and none direct relations have yet been proven between Leuconostoc isolation and human diseases ...
... The long history of safety has contributed to the acceptance of probiotics as a safe food adjunct. Consequently, many probiotic products and their applications have been granted GRAS (generally regarded as safe) status. However, this classification has been frequently generalized for all probiotic strains regardless of their application. Cases of probiotics from the genera Lactobacillus, Leuconost ...
... In the present study, solid-state fermentation of black bean with various GRAS filamentous fungi including Aspergillus awamori, Aspergillus oryzae, Aspergillus sojae, Rhizopus azygosporus and Rhizopus sp. No. 2 was preformed to prepared koji. Mycelial propagation of starter organisms and antioxidative activity, including α-α-diphenyl-2-picyl-hydoxyl (DPPH) radicals, Fe²⁺-chelating ability, and red ...
... To identify the chemical structure of a bacteriocin, thermophilin 1277, produced by Streptococcus thermophilus SBT1277. Thermophilin 1277 was purified and partial N-terminal sequence analysis revealed 6 unidentified amino acids amongst 31 amino acids residues. A 2·7-kbp region containing the thermophilin 1277 structural gene (tepA) encoding 58 amino acids was cloned and sequenced. Mature thermophi ...
... L-Arabinose isomerase (AI; E.C. 5.3.1.4), a commercial enzyme for the production of edible tagatose in vitro and ribulose production in vivo, has been studied using enzymes expressed in an Escherichia coli system, which might cause noxious by-products in food. To ensure food safety in the tagatose manufacturing process, we studied an AI expression system in Bacillus subtilis. The AI gene from Geob ...
... The exposure of foods to the temperature and humidity needed to dry the coatings can have deleterious effects on their quality. A critical analysis of the film drying conditions on quality indexes for butternut squash slices is presented. During the coatings drying, the samples lost significant amounts of weight and ascorbic acid. Only during the drying of the chitosan coating for 100 min at 20C a ...
Food and Drug Administration; GRAS substances; beverages; body composition; body weight; conjugated linoleic acid; corn oil; dietary supplements; free fatty acids; glucose; glucose tolerance; histology; humans; hypertrophy; insulin; insulin resistance; isomers; liver; mice; weight control
Abstract:
... Background Dietary supplements containing conjugated linoleic acid (CLA) are widely promoted for weight loss management over the counter. Recently, FDA approved the CLA as Generally Recognized as Safe category so that it can be used in various food and beverages. The combined effect of CLA isomers have been studied extensively in animals and humans, however, the role of individual isomers remains ...