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1. Transcriptome profiling and differential expression analysis of the immune-related genes during the acute phase of infection with Mycobacterium marinum in the goldfish (Carassius auratus L.)

Author:
Li, Chao; Jiang, Jianhu; Xie, Jiasong; Yang, Wenge; Wang, Yajun
Source:
Aquaculture 2021 v.533 pp. 736198
ISSN:
0044-8486
Subject:
Carassius auratus; Gram-positive bacteria; Mycobacterium marinum; Toll-like receptors; databases; gene expression; gene expression regulation; goldfish; host-pathogen relationships; immune response; innate immunity; models; signal transduction; spleen; transcription factor NF-kappa B; transcriptomics; unigenes
Abstract:
... Goldfish (Carassius auratus L.) have been used as a model organism to study innate immune system and host-pathogen interactions. Mycobacterium marinum is a Gram-positive bacterium that has been shown to infect many fish species. To increase knowledge of the molecular mechanisms of the host defense against M. marinum, we conducted transcriptome analysis of spleen in goldfish 24 h post-infection via ...
DOI:
10.1016/j.aquaculture.2020.736198

2. Recombinant protein and synthetic THY domain peptide of Macrobrachium rosenbergii thymosin β4 exhibit protective effect against Aeromonas hydrophila infection

Author:
Yang, Hui; Zhu, Zhu; Xiong, Haoran; Fu, Lixia; Zhang, Yingying; Chen, Guohong
Source:
Aquaculture 2021 v.532 pp. 736029
ISSN:
0044-8486
Subject:
Aeromonas hydrophila; Gram-positive bacteria; Macrobrachium rosenbergii; amino acid sequences; antimicrobial properties; antioxidant enzymes; circular dichroism spectroscopy; enzyme activity; genes; hemocytes; hepatopancreas; invertebrates; protective effect; recombinant proteins; survival rate; thymosin; vertebrates
Abstract:
... Thymosin β4 (Tβ4) is involved in cytokine secretion, nerve development, stimulation of wound healing, cancer progression, growth rate and immune response. The functions of Tβ4 in vertebrate have been widely studied, but the activities of each THY domain in invertebrate multirepeat β-thymosins remain unclear. In the present study, a new four THY domains Tβ4 gene was identified and characterized. Th ...
DOI:
10.1016/j.aquaculture.2020.736029

3. Aggregate-related microbial communities and nutrient stoichiometry under different croplands

Author:
Cui, Hu; Ou, Yang; Lv, Duian; Wang, Lixia; Liang, Aizhen; Yan, Baixing; Li, Yingxin
Source:
Ecological processes 2020 v.9 no.1 pp. 33
ISSN:
2192-1709
Subject:
Gram-negative bacteria; Gram-positive bacteria; Oryza sativa; agroecosystems; carbon nitrogen ratio; corn; crop production; cropland; fatty acid methyl esters; microbial communities; paddies; rice; soil aggregates; soil microorganisms; soybeans; stoichiometry
Abstract:
... Ecological stoichiometry is an important indicator presenting multiple elements balance in agro-ecosystems. However, information on microbial communities and nutrient stoichiometry in soil aggregate fractions under different croplands (rice, maize, and soybean fields) remains limited. Thus, this study investigated water-stable aggregate structure and their internal nutrient stoichiometry under dif ...
DOI:
10.1186/s13717-020-00239-4

4. Antimicrobial potential, phytochemical profile, cytotoxic and genotoxic screening of Sedum praealtum A. DC. (balsam)

Author:
Boriollo, Marcelo Fabiano Gomes; Marques, Milene Bueno; da Silva, Thaísla Andrielle; da Silva, Jeferson Júnior; Dias, Reigson Alves; Silva Filho, Thyago Henrique Neves; Melo, Isadora Letícia Ribeiro; dos Santos Dias, Carlos Tadeu; Bernardo, Wagner Luís de Carvalho; de Mello Silva Oliveira, Nelma; Peters, Vera Maria; Höfling, José Francisco; Spolidorio, Denise Madalena Palomari
Source:
BMC complementary medicine and therapies 2020 v.20 no.1 pp. 133
ISSN:
2662-7671
Subject:
Aedes albopictus; Bacillus cereus; Bacillus subtilis; Enterococcus faecalis; Escherichia coli; Gram-positive bacteria; Latinos; Mycobacterium tuberculosis; Proteus mirabilis; Pseudomonas aeruginosa; Salmonella Typhimurium; Sedum; Staphylococcus aureus; agar; analgesics; anti-inflammatory agents; antibacterial properties; bone marrow; cell culture; chemical constituents of plants; cytotoxicity; erythrocytes; eyes; gas chromatography-mass spectrometry; genotoxicity; mice; micronucleus tests; minimum inhibitory concentration; mutagens; roots; screening; stems; traditional medicine
Abstract:
... BACKGROUND: Sedum praealtum has been used for a long time in traditional medicine as an analgesic and anti-inflammatory agent. Its beneficial effects have been known since ancient times, when Latinos used it to treat sore and swollen eyes. This research evaluated the antimicrobial potential, the cytotoxic and genotoxic effects, and some chromatographic profiles of the hydroethanolic extract of lea ...
DOI:
10.1186/s12906-020-02915-6
PubMed:
32349729
PubMed Central:
PMC7191818

5. Evaluation of peptide-rich root extracts of Calliandria portoriscensis (Jacq.) Benth (Mimosaceae) for in vitro antimicrobial activity and brine shrimp lethality

Author:
Ogbole, Omonike O.; Ndabai, Nkiruka C.; Akinleye, Toluwanimi E.; Attah, Alfred F.
Source:
BMC complementary medicine and therapies 2020 v.20 no.1 pp. 30
ISSN:
2662-7671
Subject:
Artemia salina; Calliandra; Gram-positive bacteria; antimicrobial properties; arginine; computer software; cytotoxicity; death; drug development; dyes; gentamicin; inhibitory concentration 50; lethal concentration 50; matrix-assisted laser desorption-ionization mass spectrometry; methanol; microbial growth; molecular weight; nauplii; peptides; root extracts; roots; screening; thin layer chromatography; Germany
Abstract:
... BACKGROUND: Several Host defence peptides (HDPs) are low molecular weight (< 50 amino acids residues) peptides detected in several ethnomedicinal plants and have particularly gained research interest in recent times. Due to their wide range of bioactivity, occurrence, abundance and ability to induce very little resistance, they hold promising potentials in drug development. This study investigated ...
DOI:
10.1186/s12906-020-2836-6
PubMed:
32020886
PubMed Central:
PMC7076830

6. Exploring antibiofilm potential of bacitracin against streptococcus mutans

Author:
Zaidi, Sahar; Singh, Sneh Lata; Khan, Asad U.
Source:
Microbial pathogenesis 2020 v.149 pp. 104279
ISSN:
0882-4010
Subject:
Gram-positive bacteria; Streptococcus mutans; antimicrobial properties; bacitracin; biofilm; cell division; cell growth; cell walls; gene expression regulation; genes; gentian violet; phenotype; scanning electron microscopy; transmission electron microscopy
Abstract:
... Bacitracin has well familiar effects on growth and colonization of bacteria but its antibiofilm action on majority of bacteria is still not studied. Bacitracin is a bactericidal antibiotic that primarily acts on Gram positive bacteria by obstructing the process of cell wall synthesis. In this study, we have investigated antibiofilm potential and the mechanism of bacitracin against a cariogenic bac ...
DOI:
10.1016/j.micpath.2020.104279

7. GC-MS analysis of Myrtus communis extract and its antibacterial activity against Gram-positive bacteria

Author:
Mir, Mushtaq A.; Bashir, Nasreena; Alfaify, Abdulkhaleg; Oteef, Mohammed D. Y.
Source:
BMC complementary medicine and therapies 2020 v.20 no.1 pp. 86
ISSN:
2662-7671
Subject:
Gram-negative bacteria; Gram-positive bacteria; Myrtus communis; antibacterial properties; antibiotics; bacterial infections; branches; cell walls; computer software; essential oils; gas chromatography-mass spectrometry; growth retardation; leaf extracts; leaves; mechanism of action; microscopy; minimum inhibitory concentration; small fruits; solid phase extraction; statistical analysis; traditional medicine; Mediterranean region
Abstract:
... BACKGROUND: Myrtus communis is a typical plant of Mediterranean area. The different parts of this plant such as berries, branches, and leaves have been used worldwide as a traditional/folk medicine for the treatment of various ailments and diseases. METHODS: Ethanolic leaf extract of the plant was prepared by Soxhlet extraction method. Zone of inhibition, minimum inhibitory concentration and minim ...
DOI:
10.1186/s12906-020-2863-3
PubMed:
32183818
PubMed Central:
PMC7077171

8. GtcA is required for LTA glycosylation in Listeria monocytogenes serovar 1/2a and Bacillus subtilis

Author:
Rismondo, Jeanine; Haddad, Talal F.M.; Shen, Yang; Loessner, Martin J.; Gründling, Angelika
Source:
TheCell surface 2020 v.6 pp. 100038
ISSN:
2468-2330
Subject:
Bacillus subtilis; Gram-positive bacteria; Listeria monocytogenes; N-acetylglucosamine; cell walls; galactose; glycosylation; lipids; lipoteichoic acids; proteins; serotypes; teichoic acids; transferases
Abstract:
... The cell wall polymers wall teichoic acid (WTA) and lipoteichoic acid (LTA) are often modified with glycosyl and D-alanine residues. Recent studies have shown that a three-component glycosylation system is used for the modification of LTA in several Gram-positive bacteria including Bacillus subtilis and Listeria monocytogenes. In the L. monocytogenes 1/2a strain 10403S, the cytoplasmic glycosyltra ...
DOI:
10.1016/j.tcsw.2020.100038

9. MMPphg from the thermophilic Meiothermus bacteriophage MMP17 as a potential antimicrobial agent against both Gram-negative and Gram-positive bacteria

Author:
Wang, Feng; Xiong, Yan; Xiao, Yao; Han, Jian; Deng, Xianyu; Lin, Lianbing
Source:
Virology journal 2020 v.17 no.1 pp. 130
ISSN:
1743-422X
Subject:
Escherichia coli O157; Gram-positive bacteria; Klebsiella pneumoniae; Meiothermus; N-acetylmuramoyl-L-alanine amidase; Staphylococcus aureus; antibiotic resistance; antibiotics; antimicrobial properties; bacterial infections; bacteriophages; genes; hot springs; magnesium; multiple drug resistance; nucleotide sequences; pathogens; temperature; virulent strains; zinc; China
Abstract:
... BACKGROUND: New strategies are urgently needed to deal with the growing problem of multidrug-resistant bacterial pathogens. As the natural viruses against bacteria, recently, bacteriophages have received particular attention. Here, we identified and characterized a novel peptidoglycan hydrolase named MMPphg by decoding the complete genome sequence of Meiothermus bacteriophage MMP17, which was isol ...
DOI:
10.1186/s12985-020-01403-0

10. Renibacterium salmoninarum and Mycobacterium spp.: two bacterial pathogens present at low levels in wild brown trout (Salmo trutta fario) populations in Austrian rivers

Author:
Delghandi, M. R.; Menanteau-Ledouble, S.; Waldner, K.; El-Matbouli, M.
Source:
BMC veterinary research 2020 v.16 no.1 pp. 40
ISSN:
1746-6148
Subject:
Gram-positive bacteria; Mycobacterium; Renibacterium salmoninarum; Salmo trutta; bacterial kidney disease; chronic diseases; fish; humans; immunohistochemistry; inflammation; kidney diseases; kidneys; mycobacterial diseases; pathogens; polymerase chain reaction; rivers; summer; surveys; water temperature; Austria
Abstract:
... BACKGROUND: Renibacterium salmoninarum and Mycobacterium sp. are important bacterial pathogens of fish. R. salmoninarum is the causative agent of bacterial kidney disease, a Gram-positive bacterium mostly known for causing chronic infections in salmonid fish, while multiple species belonging to the Mycobacterium genus have been associated with mycobacteriosis in fish as well as in human. The objec ...
DOI:
10.1186/s12917-020-2260-7
PubMed:
32013968
PubMed Central:
PMC6998173

11. Conformationally tuned antibacterial oligomers target the peptidoglycan of Gram-positive bacteria

Author:
Christofferson, Andrew J.; Elbourne, Aaron; Cheeseman, Samuel; Shi, Yue; Rolland, Manon; Cozzolino, Daniel; Chapman, James; McConville, Christopher F.; Crawford, Russell J.; Wang, Peng-Yuan; Truong, Nghia P.; Anastasaki, Athina; Truong, Vi Khanh
Source:
Journal of colloid and interface science 2020 v.580 pp. 850-862
ISSN:
0021-9797
Subject:
Gram-positive bacteria; antibacterial properties; antibiotic resistance; atomic force microscopy; methicillin; methicillin-resistant Staphylococcus aureus; molecular dynamics; peptidoglycans; polymerization; polymers; simulation models
Abstract:
... The recent rise of antibiotic resistance amongst Staphylococcus aureus (S. aureus) populations has made treating Staph-based infections a global medical challenge. Therapies that specifically target the peptidoglycan layer of S. aureus have emerged as new treatment avenues, towards which bacteria are less likely to develop resistance. While the majority of antibacterial polymers/oligomers have the ...
DOI:
10.1016/j.jcis.2020.07.090

12. Deciphering Novel Antimicrobial Peptides from the Transcriptome of Papilio xuthus

Author:
Lee, Joon Ha; Chung, Hoyong; Shin, Yong Pyo; Kim, Mi-Ae; Natarajan, Sathishkumar; Veerappan, Karpagam; Kim, Seong Hyun; Park, Junhyung; Hwang, Jae Sam
Source:
Insects 2020 v.11 no.11
ISSN:
2075-4450
Subject:
Candida albicans; Escherichia coli; Gram-negative bacteria; Gram-positive bacteria; Papilio xuthus; RNA; Staphylococcus aureus; allergens; antimicrobial peptides; antimicrobial properties; butterflies; computer simulation; drug resistance; filtration; gene expression regulation; in vitro studies; innate immunity; transcriptome; unigenes; yeasts
Abstract:
... An insect’s innate immune system is the front line of defense against many invading microorganisms. One of the important components of this defense system is antimicrobial peptides (AMPs). Papiliocin is a well-studied antimicrobial peptide (AMP) isolated from the swallowtail butterfly, Papilio xuthus, and it was previously reported to be effective against Gram-positive bacteria, Gram-negative bact ...
DOI:
10.3390/insects11110776

13. The second N-terminal aromatic residue of the fungal defensin, blapersin, of Blastomyces percursus is essential for its antibacterial activity

Author:
Wu, Jiajia; Qu, Chao; Zhang, Shunliang; Chen, Wenhua; Wang, Shouwei
Source:
Peptides 2020 v.133 pp. 170400
ISSN:
0196-9781
Subject:
Blastomyces; Enterococcus faecium; Gram-positive bacteria; antibacterial properties; cell walls; fungi; genomics; lipids; molecular models; mutants; peptides; toxicity
Abstract:
... Studies have shown that the second N-terminal residue of fungal defensins is closely involved in the binding of defensins to lipid II, a bacterial cell wall precursor, and plays an important role in antibacterial activity. We found that the N-terminal residue is always aromatic in nature. In this study, 29 fungal defensin-like peptides were found via the genomic search strategy. Based on the type ...
DOI:
10.1016/j.peptides.2020.170400

14. Blade-like structure of graphene oxide sheets decorated with cuprous oxide and silicon carbide nanocomposites as bactericidal materials

Author:
Selim, Mohamed S.; Mo, Ping Jing; Hao, Zhifeng; Fatthallah, Nesreen A.; Chen, Xiang
Source:
Journal of colloid and interface science 2020 v.578 pp. 698-709
ISSN:
0021-9797
Subject:
Bacillus subtilis; Brevibacillus brevis; Candida albicans; Escherichia coli; Gram-positive bacteria; Pseudomonas aeruginosa; antibacterial properties; antibiotics; aqueous solutions; cell membranes; cell viability; comparative study; cuprous oxide; graphene oxide; minimum inhibitory concentration; nanocomposites; nanosheets; nanospheres; nanowires; scanning electron microscopy; silicon carbide; toxicity; yeasts
Abstract:
... In the current work, graphene oxide nanosheets decorated with cuprous oxide nanospheres (GO/Cu₂O) and with silicon carbide nanowires (GO/SiC) were synthesized and controlled for using as blade-like antibacterial agents. Preparation of GO sheets with <2 nm thickness was performed through a modified Hummers approach. Controlled Cu₂O spheres with a mean size of 40 nm were prepared through a wet-chemi ...
DOI:
10.1016/j.jcis.2020.06.058

15. Comparative antioxidant and antimicrobial potentials of leaf successive extract fractions of poison bulb, Crinum asiaticum L.

Author:
Goswami, Souren; Das, Ria; Ghosh, Poulomi; Chakraborty, Tista; Barman, Anuva; Ray, Sanjib
Source:
Industrial crops and products 2020 v.154 pp. 112667
ISSN:
0926-6690
Subject:
2,2-diphenyl-1-picrylhydrazyl; Crinum asiaticum; Gram-positive bacteria; anti-infective agents; antimicrobial properties; antioxidant activity; bioactive compounds; bulbs; ethyl acetate; flavonoids; free radical scavengers; iron; leaf extracts; leaves; medicinal plants; solvents; terpenoids
Abstract:
... Crinum asiaticum L. (family: Amaryllidaceae) is a widely used medicinal plant species having great ethnomedicinal values. The present study aimed to evaluate in vitro antioxidant and antimicrobial potentials of the leaf extracts of C. asiaticum with their phenolics content. The total antioxidant assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, and ferric ion reducing a ...
DOI:
10.1016/j.indcrop.2020.112667

16. Linden tea from Serbia – an insight into the phenolic profile, radical scavenging and antimicrobial activities

Author:
Pavlović, Tamara; Dimkić, Ivica; Andrić, Snežana; Milojković-Opsenica, Dušanka; Stanković, Slaviša; Janaćković, Peđa; Gavrilović, Milan; Ristivojević, Petar
Source:
Industrial crops and products 2020 v.154 pp. 112639
ISSN:
0926-6690
Subject:
2,2-diphenyl-1-picrylhydrazyl; 4-hydroxybenzoic acid; Bacillus subtilis; Candida albicans; Candida glabrata; Gram-positive bacteria; Staphylococcus aureus; Streptococcus mutans; Streptococcus pyogenes; Tilia cordata; antimicrobial properties; antioxidant activity; caffeic acid; chlorogenic acid; free radical scavengers; isomers; isorhamnetin; luteolin; mass spectrometry; metabolites; minimum inhibitory concentration; mouth; procyanidins; quercitrin; rutin; tea; thin layer chromatography; traditional medicine; Serbia
Abstract:
... Linden tea has been used in Serbian folk medicine for centuries to induce sweating for colds, relieve throat irritation and cough, reduce blood pressure, as well as a diuretic, spasmolytic, and as a sedative. The main aim of this study was to determine the comprehensive metabolite profile, free radical scavenging activity, and antimicrobial activities against 23 human and plant pathogens of both c ...
DOI:
10.1016/j.indcrop.2020.112639

17. Potential application of grape (Vitis vinifera L.) stem extracts in the cosmetic and pharmaceutical industries: Valorization of a by-product

Author:
Leal, Carla; Gouvinhas, Irene; Santos, Rafaela A.; Rosa, Eduardo; Silva, Amélia M.; Saavedra, Maria José; Barros, Ana I.R.N.A.
Source:
Industrial crops and products 2020 v.154 pp. 112675
ISSN:
0926-6690
Subject:
2,2-diphenyl-1-picrylhydrazyl; Gram-positive bacteria; Vitis vinifera; anti-aging properties; antimicrobial properties; antioxidant activity; antioxidants; byproducts; catechin; cosmetics; grapes; high performance liquid chromatography; macrophages; new products; pharmaceutical industry; stem extracts; wastes; wine industry
Abstract:
... Wastes from the wine industry can cause sustainably problems, thus requiring their reuse. Thus, new products can be generated through these wastes, giving environmental, social, and economic advantages. In this sense, the objective of this work was to determine the phenolic composition of six varieties of grape stem extracts by High Performance Liquid Chromatography and to evaluate their biologica ...
DOI:
10.1016/j.indcrop.2020.112675

18. Nisin-loaded polydopamine/hydroxyapatite composites: Biomimetic synthesis, and in vitro bioactivity and antibacterial activity evaluations

Author:
Wang, Ni; Yu, Xing; Kong, Qingjun; Li, Zhenni; Li, Penghui; Ren, Xueyan; Peng, Bo; Deng, Ziwei
Source:
Colloids and surfaces 2020 v.602 pp. 125101
ISSN:
0927-7757
Subject:
Gram-positive bacteria; Staphylococcus aureus; T-lymphocytes; X-ray diffraction; X-ray photoelectron spectroscopy; antibacterial properties; bacterial adhesion; biocompatible materials; biomimetic synthesis; crystal structure; dopamine; drugs; embryo (animal); energy-dispersive X-ray analysis; engineering; human health; humans; hydroxyapatite; infrared spectroscopy; kidneys; microbial contamination; mineralization; moieties; nisin; scanning electron microscopy; solvents; transmission electron microscopy
Abstract:
... Microbial contamination caused by bacterial adhesion and colonization onto the materials surfaces has long been a serious threat to human health. Thus, it is imperative to develop advanced antimicrobial materials for bio-applications. Here, a unique polydopamine/ hydroxyapatite/nisin (PDA/HAP/Nisin) composite with good bioactivities and enhanced antibacterial activities have been developed. The un ...
DOI:
10.1016/j.colsurfa.2020.125101

19. Synthesis of Ag-AgCl nanoparticles capped by calix[4]resorcinarene-mPEG conjugate and their antimicrobial activity

Author:
Shumatbaeva, Alina M.; Morozova, Julia E.; Shalaeva, Yana V.; Saifina, Alina F.; Gubaidullin, Aidar T.; Syakaev, Victor V.; Sapunova, Anastasia S.; Voloshina, Alexandra D.; Nizameev, Irek R.; Kadirov, Marsil K.; Bulygina, Kseniya S.; Babaev, Vasily M.; Antipin, Igor S.
Source:
Colloids and surfaces 2020 v.602 pp. 125124
ISSN:
0927-7757
Subject:
Fourier transform infrared spectroscopy; Gram-negative bacteria; Gram-positive bacteria; X-ray fluorescence spectroscopy; antibiotics; antimicrobial properties; fungi; mechanism of action; nanoparticles; nanosilver; physicochemical properties; reducing agents; silver; silver chloride; sodium borohydride; transmission electron microscopy; ultraviolet-visible spectroscopy
Abstract:
... The growing resistance of bacteria to traditional antibiotics makes it necessary to develop new antimicrobial agents with the dissimilar mode of action such as Ag and AgCl nanoparticles. Here the synthesis of silver NPs in the colloidal solutions of calix[4] resorcinarene-mPEG conjugate C₁₁-mPEG was reported. NPs were synthesized under varied conditions: different Ag⁺/C₁₁-mPEG molar ratio, presenc ...
DOI:
10.1016/j.colsurfa.2020.125124

20. Chemical profiling and bioactivity of essential oils from Alpinia officinarum Hance from ten localities in China

Author:
Zhang, Lanyue; Pan, Chunxing; Ou, Zhirong; Liang, Xiaoxin; Shi, Yaohui; Chi, Lejing; Zhang, Zejun; Zheng, Xi; Li, Chunlian; Xiang, Hongping
Source:
Industrial crops and products 2020 v.153 pp. 112583
ISSN:
0926-6690
Subject:
Alpinia officinarum; Gram-positive bacteria; additives; alpha-terpineol; anti-inflammatory activity; antibacterial properties; antineoplastic activity; cell lines; cineole; drugs; ears; edema; essential oils; gas chromatography-mass spectrometry; habitats; herbs; inhibitory concentration 50; interleukin-1beta; mice; nutrition; rhizomes; tumor necrosis factor-alpha; China
Abstract:
... Essential oil of herbs has natural bioactivity, which can be applied on biomedical products, pharmaceutical preparations, natural functional nutrition and cosmetic additives. The chemical components and bioactivities of essential oils extracted from Alpinia officinarum Hance rhizomes collected from ten various habitats in China were comparatively investigated. The yield of ten essential oils varie ...
DOI:
10.1016/j.indcrop.2020.112583