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1. Cladistic analysis of iridoviruses based on protein and DNA sequences

Author:
Wang, J.W.; Deng, R.Q.; Wang, X.Z.; Huang, Y.S.; Xing, K.; Feng, J.H.; He, J.G.; Long, Q.X.
Source:
Archives of virology 2003 v.148 no.11 pp. 2181-2194
ISSN:
0304-8608
Subject:
coat proteins; insect viruses; vertebrate viruses; taxonomy; phylogeny; amino acid sequences; Infectious spleen and kidney necrosis virus; nucleotide sequences
DOI:
10.1007/s00705-003-0178-6
PubMed:
14579177

2. Multiplex PCR for the diagnosis of red sea bream iridoviruses isolated in Korea

Author:
Jeong, J.B.; Park, K.H.; Kim, H.Y.; Hong, S.H.; Kim, K.H.; Chung, J.K.; Komisar, J.L.; Jeong, H.D.
Source:
Aquaculture 2004 v.235 no.1-4 pp. 139-152
ISSN:
0044-8486
Subject:
fish farms; Iridovirus; viral proteins; ribonucleotide reductase; adenosinetriphosphatase; DNA-directed DNA polymerase; genetic variation; viral diseases of animals and humans; fish diseases; disease diagnosis; polymerase chain reaction; Lateolabrax; perch; marine fish; restriction endonucleases; fish culture; gene amplification; nucleotide sequences; Infectious spleen and kidney necrosis virus; South Korea; China
Abstract:
... Amplification by the polymerase chain reaction (PCR) was done to determine the presence of red sea bream iridovirus (RSIV) in sea perch (Lateolabrax sp.) imported from China, targeting four genomic regions, the ribonucleotide reductase small subunit (RNRS) gene, the adenosine triphosphatase (ATPase) gene, the DNA polymerase (DPOL) gene, and the Pst I restriction fragment, which have been considere ...

3. Characterization of a membrane protein (VP001L) from infectious spleen and kidney necrosis virus (ISKNV)

Author:
Xiaopeng Xu; Jing Lu; Qingxia Lu; Hui Zhong; Shaoping Weng; Jianguo He
Source:
Virus genes 2008 v.36 no.1 pp. 157-167
ISSN:
0920-8569
Subject:
messenger RNA; open reading frames; models; transfection; membrane proteins; Infectious spleen and kidney necrosis virus; hydrophobicity; mutants; plasma membrane; start codon; topology
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) is the type species of megalocytivirus, Iridoviridae. A novel membrane protein corresponding to the first open reading frame (ORF001L) of ISKNV genome was identified. This 378-residue protein, termed the VP001L protein, has a high content of hydrophobic sequences and contains 10–11 putative transmembrane domains, indicating it may be a membrane p ...
DOI:
10.1007/s11262-007-0177-x
PubMed:
18046636

4. Molecular cloning of two C1q-like cDNAs in mandarin fish Siniperca chuatsi

Author:
Lao, Hai-Hua; Sun, Ya-Nan; Yin, Zhi-Xin; Wang, Jing; Chen, Chao; Weng, Shao-Ping; He, Wei; Guo, Chang-Jun; Huang, Xian-De; Yu, Xiao-Qiang; He, Jian-Guo
Source:
Veterinary immunology and immunopathology 2008 v.125 no.1-2 pp. 37-46
ISSN:
0165-2427
Subject:
molecular cloning; freshwater fish; Percichthyidae; circular DNA; amino acid sequences; viral diseases of animals and humans; Rhabdoviridae; Infectious spleen and kidney necrosis virus
Abstract:
... C1q, a subunit of the C1 complex, plays a key role in the recognition of immune complexes to initiate the classical complement pathway. In this study, we reported two C1q-like cDNAs from mandarin fish (Siniperca chuatsi), mC1q-like-1 (mC1qL1) and mC1q-like-2 (mC1qL2). The full-length cDNA of mC1qL1was 990bp, containing a 71bp 5'-untranslated region (UTR), an open reading frame (ORF) of 723bp, and ...
DOI:
10.1016/j.vetimm.2008.05.004
PubMed:
18571244

5. Cloning, characterization and expression analysis of a CXCR1-like gene from mandarin fish Siniperca chuatsi

Author:
Chen, Chao; Li, Zhongsheng; Zhou, Zhicheng; Yin, Zhixin; Chan, Siu-Ming; Yu, Xiao-Qiang; Weng, Shaoping; He, Jianguo
Source:
Fish physiology and biochemistry 2009 v.35 no.3 pp. 489-499
ISSN:
0920-1742
Subject:
nucleotides; 3' untranslated regions; fish; blood; promoter regions; 5' untranslated regions; open reading frames; clones; mandarins; amino acids; Infectious spleen and kidney necrosis virus; spleen; start codon; transcription factors; complementary DNA; kidneys; liver; binding sites; gills
Abstract:
... In this study we cloned and characterized a CXCR1-like gene (mfCXCR1) from mandarin fish (Siniperca chuatsi). The full-length cDNA of mfCXCR1 is 2,173 bp and contains a 1,056 bp open reading frame (ORF) that encodes a protein of 351 amino acids. The 5' and 3' untranslated regions (UTR) are 57 and 1,080 bp in length, respectively. The coding region of the mfCXCR1 gene consists of a single exon with ...
DOI:
10.1007/s10695-008-9283-5
PubMed:
19093223

6. Phenotypic Diversity of Infectious Red Sea Bream Iridovirus Isolates from Cultured Fish in Japan

Author:
Shinmoto, Hajime; Taniguchi, Ken; Ikawa, Takuya; Kawai, Kenji; Oshima, Syun-ichirou
Source:
Applied and environmental microbiology 2009 v.75 no.11 pp. 3535-3541
ISSN:
0099-2240
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; Pagrus major; amino acid sequences; bromides; cell culture; coat proteins; death; disease outbreaks; farmed fish; fish farms; host range; in vitro studies; in vivo studies; mortality; phylogeny; polymerase chain reaction; strain differences; vaccines; viability; virulence; viruses; Japan
Abstract:
... Megalocytivirus is causing economically serious mass mortality by infecting fish in and around the Pacific region of Asia. The recent emergence of many new iridoviruses has drawn attention to the marked taxonomic variation within this virus family. Most studies of these viruses have not included extensive study of these emergent species. We explored the emergence of red sea bream iridovirus (RSIV) ...
DOI:
10.1128/AEM.02255-08
PubMed:
19346349
PubMed Central:
PMC2687316

7. Identification of the VP92R gene from infectious spleen and kidney necrosis virus

Author:
Xu, Xiaopeng; Lu, Qingxia; Wang, Rui; Lin, Ting; Weng, Shaoping; Lu, Ling; Li, Zhongsheng; Yu, Xiaoqiang; Tang, Junliang; He, Jianguo
Source:
Virus genes 2010 v.41 no.2 pp. 210-217
ISSN:
0920-8569
Subject:
sequence analysis; Iridovirus; Larimichthys crocea; genes; viruses; fish; pathogenesis; molecular weight; Epinephelus coioides; fluorescent antibody technique; Rock bream iridovirus
Abstract:
... Megalocytiviruses, which belong to the family Iridoviridae, are among the most harmful viruses to cultured fishes. Infectious spleen and kidney necrosis virus (ISKNV) is the type species of the Megalocytivirus genus. The genome of ISKNV was sequenced and annotated by He et al. (Virology 291:126-139, 2001) in 2001 and re-annotated by Eaton et al. (Virol. J. 4:11, 2007) in 2007. The ORF092R of ISKNV ...
DOI:
10.1007/s11262-010-0502-7
PubMed:
20577793

8. Rapid and sensitive detection of infectious spleen and kidney necrosis virus by loop-mediated isothermal amplification combined with a lateral flow dipstick

Author:
Ding, W. C.; Chen, Jiong; Shi, Y. H.; Lu, X. J.; Li, M. Y.
Source:
Archives of virology 2010 v.155 no.3 pp. 385-389
ISSN:
0304-8608
Subject:
DNA; Infectious spleen and kidney necrosis virus; biotin; chromatography; detection limit; genes; polymerase chain reaction
Abstract:
... Loop-mediated isothermal amplification (LAMP) allows rapid amplification of nucleic acids under isothermal conditions. In this report, a 20-min LAMP amplification of the DPOL gene of infectious spleen and kidney necrosis virus (ISKNV) using a biotin-labeled primer was combined with lateral flow dipstick (LFD) chromatography for rapid and simple visual detection of ISKNV-specific amplicons. The LFD ...
DOI:
10.1007/s00705-010-0593-4
PubMed:
20107846

9. Antigenic identification of virion structural proteins from infectious spleen and kidney necrosis virus

Author:
Xiao-Peng Xiong; Chuan-Fu Dong; Shao-Ping Weng; Jing Zhang; Ye Zhang; Jian-Guo He
Source:
Fish & shellfish immunology 2011 v.31 no.6 pp. 919-924
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Western blotting; antiserum; coat proteins; financial economics; fish culture; freshwater fish; gel electrophoresis; immune response; industry; mortality; neutralization; proteome; proteomics; structural proteins; vaccines; virion; Asia
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV), belonging to the genus Megalocytivirus in the family Iridoviridae, is one of the major agents causing mortality and economic losses to the freshwater fish culture industry in Asian countries. Currently, little information regarding the antigenic properties of Megalocytivirus (especially ISKNV) is available. Our previous study using four differen ...
DOI:
10.1016/j.fsi.2011.08.009

10. Cloning, characterization, and biological function analysis of the SidT2 gene from Siniperca chuatsi

Author:
Ruiwen Ren; Xiaopeng Xu; Ting Lin; Shaoping Weng; Huafang Liang; Mengli Huang; Chuanfu Dong; Yongwen Luo; Jianguo He
Source:
Developmental and comparative immunology 2011 v.35 no.6 pp. 692-701
ISSN:
0145-305X
Subject:
Frog virus 3; Infectious spleen and kidney necrosis virus; Pimephales promelas; RNA interference; Siniperca chuatsi; antiserum; double-stranded RNA; epithelial cells; fish fry; gene overexpression; genes; messenger RNA; open reading frames; reproduction; signal peptide; tissue distribution; viruses
Abstract:
... The systemic RNA interference defective protein (SID)-1 plays an important role in dsRNA uptake in cells. We identified the ScSidT2 gene from the mandarin fish (Siniperca chuatsi), which is the first-studied SID-1 homolog in fish. ScSidT2 mRNA is 3380bp long and contains a 2568-bp open reading frame that encodes an 855-amino-acid protein with an N-terminal signal peptide and ten putative transmemb ...
DOI:
10.1016/j.dci.2011.02.003

11. Genotype and host range analysis of infectious spleen and kidney necrosis virus (ISKNV)

Author:
Xiaozhe Fu; Ningqiu Li; Lihui Liu; Qiang Lin; Fang Wang; Yingtiao Lai; Haiming Jiang; Houjun Pan; Cunbin Shi; Shuqin Wu
Source:
Virus genes 2011 v.42 no.1 pp. 97-109
ISSN:
0920-8569
Subject:
Infectious spleen and kidney necrosis virus; capsid; freshwater fish; genes; genetic variation; genotype; host range; marine fish; mixing; mortality; nucleotide sequences; phylogeny; viruses
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) is the causative agent of a disease causing high mortality in mandarin fish, Siniperca chuatsi. In this study, complete major capsid protein (MCP) genes of nine ISKNV isolates were sequenced and compared with other known megalocytiviruses to evaluate genetic variation and host range of the viruses. Comparison of nucleotide sequences of MCP gene r ...
DOI:
10.1007/s11262-010-0552-x
PubMed:
21107672

12. Involvement of caveolin-1 in the Jak–Stat signaling pathway and infectious spleen and kidney necrosis virus infection in mandarin fish (Siniperca chuatsi)

Author:
Chang-Jun Guo; Xiao-Bo Yang; Yan-Yan Wu; Li-Shi Yang; Shu Mi; Zhao-Yu Liu; Kun-Tong Jia; Yu-Xin Huang; Shao-Ping Weng; Xiao-Qiang Yu; Jian-Guo He
Source:
Molecular immunology 2011 v.48 no.8 pp. 992-1000
ISSN:
0161-5890
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; amino acid sequences; amino acids; complementary DNA; dose response; endocytosis; fish fry; genes; immune system; mammals; nucleotide sequences; phosphorylation; plasma membrane; proteins; quantitative polymerase chain reaction; signal transduction; viruses
Abstract:
... Caveolae, the major source of caveolin-1 protein, are specialized invaginated microdomains of the plasma membrane that act as organizing centers for signaling molecules in the immune system. In the present study, we report the cloning and characterization of caveolin-1 (mCav-1) from mandarin fish (Siniperca chuatsi) and study on the roles of mCav-1 in the fish Jak–Stat signaling pathway and in vir ...
DOI:
10.1016/j.molimm.2011.01.001
PubMed:
21296425
PubMed Central:
PMC7112660

13. Molecular characterisation and expression analysis of a fish-egg lectin in rock bream, and its response to bacterial or viral infection

Author:
Bong-Seok Kim; Bo-Hye Nam; Ju-Won Kim; Hyoung-Jun Park; Jeong-Hun Song; Chan-Il Park
Source:
Fish & shellfish immunology 2011 v.31 no.6 pp. 1201-1207
ISSN:
1050-4648
Subject:
Edwardsiella tarda; Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Streptococcus iniae; bream; carbohydrate binding; complementary DNA; cysteine; eggs; expressed sequence tags; innate immunity; invertebrates; kidneys; lectins; liver; messenger RNA; open reading frames; polypeptides; quantitative polymerase chain reaction; reverse transcription; time series analysis; tissues
Abstract:
... A fish-egg lectin, RbFEL, was identified from rock bream (Oplegnathus fasciatus) and its expression analysed. In both vertebrates and invertebrates, carbohydrate-binding proteins (lectins) play an important role in innate immunity against microbial invasion. Here, we report the cloning of a fish-egg lectin from rock bream using a combination of expression sequence tag (EST) analyses. The full-leng ...
DOI:
10.1016/j.fsi.2011.10.024

14. Molecular identification and expression analysis of cathepsins O and S from rock bream, Oplegnathus fasciatus

Author:
Ju-Won Kim; Ji-Min Jeong; Hyoung-Jun Park; Eun-Gyeong Kim; Ha-Na Kim; Young-Sun Chae; Do-Hyung Kim; Chan-Il Park
Source:
Fish & shellfish immunology 2011 v.31 no.4 pp. 578-587
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Streptococcus iniae; amino acids; bream; cDNA libraries; cathepsin O; complementary DNA; expressed sequence tags; gene expression; gills; homeostasis; kidneys; lipopolysaccharides; liver; lymphocytes; messenger RNA; metabolism; muscles; open reading frames; papain; reverse transcriptase polymerase chain reaction; spleen; tissues
Abstract:
... Cathepsins are lysosomal cysteine proteases belonging to the papain family, members of which play important roles in normal metabolism for maintenance of cellular homeostasis. Rock bream (Oplegnathus fasciatus) cathepsin O and S (RbCTSO and RbCTSS, respectively) cDNAs were identified by expressed sequence tag (EST) analysis of a lipopolysaccharide (LPS)-stimulated rock bream liver cDNA library. Th ...
DOI:
10.1016/j.fsi.2011.07.007

15. Proteomic analysis of zebrafish (Danio rerio) infected with infectious spleen and kidney necrosis virus

Author:
Xiao-Peng Xiong; Chuan-Fu Dong; Xiaopeng Xu; Shao-Ping Weng; Zhao-Yu Liu; Jian-Guo He
Source:
Developmental and comparative immunology 2011 v.35 no.4 pp. 431-440
ISSN:
0145-305X
Subject:
Danio rerio; Infectious spleen and kidney necrosis virus; Iridovirus; Western blotting; actin; aquaculture; biosynthesis; carbohydrate metabolism; coat proteins; cytoskeleton; financial economics; freshwater fish; gene expression regulation; genes; industry; lipoproteins; mass spectrometry; oxidation; pathogenesis; protein synthesis; proteolysis; proteome; proteomics; reverse transcriptase polymerase chain reaction; signal transduction; spleen; stress response; tubulin; two-dimensional gel electrophoresis; Asia
Abstract:
... Iridovirus infections remain a severe problem in aquaculture industries worldwide. Infectious spleen and kidney necrosis virus (ISKNV), the type species of the genus Megalocytivirus in the family Iridoviridae, has caused significant economic losses among freshwater fish in different Asian countries. To investigate the molecular mechanism of iridoviral pathogenesis, we analyzed the differential pro ...
DOI:
10.1016/j.dci.2010.11.006

16. VP15R from infectious spleen and kidney necrosis virus is a non-muscle myosin-II-binding protein

Author:
Xu, Xiaopeng; Lin, Ting; Huang, Lichao; Weng, Shaoping; Wei, Wei; Li, Zhongsheng; Lü, Ling; Huang, Zhijian; He, Jianguo
Source:
Archives of virology 2011 v.156 no.1 pp. 53-61
ISSN:
0304-8608
Subject:
Danio rerio; Infectious spleen and kidney necrosis virus; cytoplasm; filamin; fish; fluorescent antibody technique; humans; messenger RNA; mice; myosin; nuclear localization signals; open reading frames; start codon
Abstract:
... VP15R protein, encoded by the 15th open reading frame of infectious spleen and kidney necrosis virus (ISKNV), was identified. VP15R is a 263-residue protein that is first transcribed within 12 h post-infection. The VP15R mRNA is transcribed beginning at ISKNV genomic coordinate 12111, extending 167 bp upstream of the initiation codon. No signal peptides, transmembrane fragments, or nuclear localiz ...
DOI:
10.1007/s00705-010-0815-9
PubMed:
20882305

17. Development of a quantitative polymerase chain reaction (qPCR) assay for the detection of dwarf gourami iridovirus (DGIV) and other megalocytiviruses and comparison with the Office International des Epizooties (OIE) reference PCR protocol

Author:
Anneke E. Rimmer; Joy A. Becker; Alison Tweedie; Richard J. Whittington
Source:
Aquaculture 2012 v.358-359 no. pp. 155-163
ISSN:
0044-8486
Subject:
DNA; Infectious spleen and kidney necrosis virus; detection limit; fish diseases; ornamental fish; pathogens; polymerase chain reaction; quantitative analysis; quarantine; reference standards; screening; viruses; Australia
Abstract:
... Viruses in the genus Megalocytivirus (Family Iridoviridae) have emerged as a source of significant disease and mortality in fish worldwide. Although regarded as exotic in Australia, the importation of ornamental fish provides a transmission pathway for the introduction of a megalocytivirus, dwarf gourami iridovirus (DGIV), which is a strain of infectious spleen and kidney necrosis virus (ISKNV). T ...
DOI:
10.1016/j.aquaculture.2012.06.034

18. Immunological evaluation of Vibrio alginolyticus, Vibrio harveyi, Vibrio vulnificus and infectious spleen and kidney necrosis virus (ISKNV) combined-vaccine efficacy in Epinephelus coioides

Author:
Zhijian Huang; Jingjing Tang; Mei Li; Yacheng Fu; Chuanfu Dong; Jiang F. Zhong; Jianguo He
Source:
Veterinary immunology and immunopathology 2012 v.150 no.1-2 pp. 61-68
ISSN:
0165-2427
Subject:
Epinephelus coioides; Infectious spleen and kidney necrosis virus; Vibrio alginolyticus; Vibrio harveyi; Vibrio vulnificus; antibacterial properties; antibodies; grouper; head; immunization; infectious diseases; kidney cells; leukocytes; lysozyme; pathogens; splenocytes; vaccines
Abstract:
... Combined vaccines are immunological products intended for immunization against multifactorial infectious diseases caused by different types or variants of pathogens. In this study, the effectiveness of Vibrio alginolyticus (Va), Vibrio harveyi (Vh), Vibrio vulnificus (Vv) and infectious spleen and kidney necrosis virus (ISKNV), an iridovirus, combined-vaccine (Vibrio and ISKNV combined vaccines, V ...
DOI:
10.1016/j.vetimm.2012.08.008
PubMed:
23010220

19. Molecular identification and expression analysis of two distinct BPI/LBPs (bactericidal permeability-increasing protein/LPS-binding protein) from rock bream, Oplegnathus fasciatus

Author:
Ju-Won Kim; Lena Gerwick; Chan-Il Park
Source:
Fish & shellfish immunology 2012 v.33 no.1 pp. 75-84
ISSN:
1050-4648
Subject:
lipopolysaccharides; Streptococcus iniae; bream; open reading frames; leukocytes; gene expression; amino acids; Infectious spleen and kidney necrosis virus; intestines; spleen; Edwardsiella tarda; messenger RNA; complementary DNA; Oplegnathus fasciatus; cDNA libraries; kidneys; translation (genetics); liver; reverse transcriptase polymerase chain reaction; expressed sequence tags
Abstract:
... We identified two cDNAs designated as RbBPI/LBP-1 and RbBPI/LBP2, respectively, which were identified by expressed sequence tag (EST) analysis of a lipopolysaccharide (LPS)-stimulated rock bream liver cDNA library. The two cDNA displayed 36.9% identity at the translated amino acid level. Despite the low level of identity between the two genes, high conservation was seen in the BPI/LBP/CETP N-termi ...
DOI:
10.1016/j.fsi.2012.04.003

20. Protective immunity against iridovirus disease in mandarin fish, induced by recombinant major capsid protein of infectious spleen and kidney necrosis virus

Author:
Xiaozhe Fu; Ningqiu Li; Yingtiao Lai; Lihui Liu; Qiang Lin; Cunbin Shi; Zhibin Huang; Shuqin Wu
Source:
Fish & shellfish immunology 2012 v.33 no.4 pp. 880-885
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; Western blotting; coat proteins; dialysis; financial economics; fish; genes; humoral immunity; intraperitoneal injection; kidneys; lymphocyte proliferation; molecular weight; mortality; polyclonal antibodies; rabbits; recombinant proteins; spleen; temperature; vaccine development; vaccines; China
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) is the causative agent of a disease causing high mortality and economic losses in mandarin fish, Siniperca chuatsi in China. But little information about vaccine development against ISKNV disease is available. In this study the gene encoding the major capsid protein (MCP), which is predominant structural component of the iridovirus particles, was ...
DOI:
10.1016/j.fsi.2012.07.012

21. Efficacy of a formalin-killed cell vaccine against infectious spleen and kidney necrosis virus (ISKNV) and immunoproteomic analysis of its major immunogenic proteins

Author:
Chuanfu Dong; Xiaopeng Xiong; Yongwen Luo; Shaoping Weng; Qing Wang; Jianguo He
Source:
Veterinary microbiology 2013 v.162 no.2-4 pp. 419-428
ISSN:
0378-1135
Subject:
Infectious spleen and kidney necrosis virus; Western blotting; antibodies; coat proteins; control methods; etiological agents; fish; fish industry; fluorescent antibody technique; immunity; immunoglobulin M; mass spectrometry; neutralization; recombinant proteins; two-dimensional gel electrophoresis; vaccines; virion; viruses; China
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV), the type species of genus Megalocytivirus in the family Iridoviridae, is the most important etiological agents in mandarin fish industry in China. Since its first occurrence in China in the early 1990s, there is no effective method to prevent and control this virus. Here we report the successful development of a formalin-killed cell-cultured (FK ...
DOI:
10.1016/j.vetmic.2012.10.026
PubMed:
23177910

22. Molecular characterisation and biological activity of a novel CXC chemokine gene in rock bream (Oplegnathus fasciatus)

Author:
Ju-Won Kim; Eun-Gyeong Kim; Do-Hyung Kim; Sang Hee Shim; Chan-Il Park
Source:
Fish & shellfish immunology 2013 v.34 no.5 pp. 1103-1111
ISSN:
1050-4648
Subject:
chemoattractants; lipopolysaccharides; phylogeny; mammals; chemokines; Streptococcus iniae; bream; open reading frames; recombinant fusion proteins; binding proteins; gene expression; leukocytes; Infectious spleen and kidney necrosis virus; spleen; Edwardsiella tarda; cysteine; tissues; Escherichia coli; messenger RNA; complementary DNA; Oplegnathus fasciatus; cDNA libraries; kidneys; liver; bioactive properties; vaccines; expressed sequence tags; maltose; polyinosinic-polycytidylic acid; chemotaxis
Abstract:
... Chemokines are chemoattractant cytokines defined by the presence of four conserved cysteine residues. In mammals, these cytokines can be divided into four subfamilies depending on the arrangement of the first two conserved cysteines in the sequence, and include the CXC(α), CC(β), C(γ), and CX3C(δ) classes. We identified CXC chemokine cDNA, designated RbCXC, isolated using expressed sequence tag an ...
DOI:
10.1016/j.fsi.2013.01.007

23. Molecular characterisation and expression analysis of the cathepsin H gene from rock bream (Oplegnathus fasciatus)

Author:
Ju-Won Kim; Chan-Il Park; Seong Don Hwang; Ji-Min Jeong; Ki-Hyuk Kim; Do-Hyung Kim; Sang Hee Shim
Source:
Fish & shellfish immunology 2013 v.35 no.1 pp. 188-194
ISSN:
1050-4648
Subject:
phylogeny; Streptococcus iniae; bream; open reading frames; Oreochromis niloticus; gene expression; amino acids; Infectious spleen and kidney necrosis virus; intestines; homeostasis; spleen; stomach; Edwardsiella tarda; cathepsin H; bacterial infections; pathogens; tissues; messenger RNA; metabolism; complementary DNA; Oplegnathus fasciatus; cDNA libraries; kidneys; papain; liver; innate immunity; expressed sequence tags
Abstract:
... Cathepsins are lysosomal cysteine proteases belonging to the papain family, whose members play important roles in normal metabolism for the maintenance of cellular homeostasis. Rock bream (Oplegnathus fasciatus) cathepsin H (RbCTSH) cDNAs were identified by expressed sequence tag analysis of a lipopolysaccharide-stimulated rock bream liver cDNA library. The full-length RbCTSH cDNA (1326 bp) contai ...
DOI:
10.1016/j.fsi.2013.04.016

24. Persistently betanodavirus-infected barramundi (Lates calcarifer) exhibit resistances to red sea bream iridovirus infection

Author:
Yu-Chi Wu; Yu-Hsuan Kai; Shau-Chi Chi
Source:
Developmental and comparative immunology 2013 v.41 no.4 pp. 666-674
ISSN:
0145-305X
Subject:
Betanodavirus; Infectious spleen and kidney necrosis virus; Lates calcarifer; brain; cell culture; farmed fish; gene expression; interferons; juveniles; larvae; liver; mortality; pathogenicity; pathogens; polyclonal antibodies; progeny
Abstract:
... Nervous necrosis virus (NNV) and red sea bream iridovirus (RSIV) are two important pathogens that have caused acute, highly contagious, and widespread diseases among wild and cultured fish, especially at larval and juvenile stages. We discovered that the pathogenicity of NNV to the 80days post-hatch (dph) barramundi is lower than that to the 14dph barramundi. Following NNV challenge, no mortality ...
DOI:
10.1016/j.dci.2013.06.011

25. A loop-mediated isothermal amplification method for the detection of members of the genus Ranavirus

Author:
Zhang Min; Jing Hongli; Zhang Lifeng; Wang Na; Wu Shaoqiang; Lin Xiangmei
Source:
Archives of virology 2013 v.158 no.10 pp. 2121-2126
ISSN:
0304-8608
Subject:
DNA; DNA-directed DNA polymerase; Ictalurid herpesvirus 1; Infectious hematopoietic necrosis virus; Infectious spleen and kidney necrosis virus; Iridovirus; Ranavirus; White spot syndrome virus; buffers; detection limit; polymerase chain reaction; viruses
Abstract:
... A loop-mediated isothermal amplification (LAMP) method was developed for detection of members of the genus Ranavirus. The optimum reaction mixture contained 2.5 μL of each inner primer, RV-FIP (20 pmol/μL) and RV-BIP (20 pmol/μL), 0.5 μL of each outer primer, RV-F3 (10 pmol/μL) and RV-B3 (10 pmol/μL), 1.25 μL of each loop primer, RV-LF (20 pmol/μL) and RV-LB (20 pmol/μL), 3.5 μL dNTP mix (10 mM ea ...
DOI:
10.1007/s00705-013-1724-5
PubMed:
23665768

26. The potential role of microfilaments in host cells for infection with infectious spleen and kidney necrosis virus infection

Author:
Kun-tong Jia; Zhao-yu Liu; Chang-jun Guo; Qiong Xia; Shu Mi; Xiao-Dong Li; Shao-ping Weng; Jian-guo He
Source:
Virology journal 2013 v.10 no.1 pp. 77
ISSN:
1743-422X
Subject:
Infectious spleen and kidney necrosis virus; actin; cytochalasin B; cytochalasin D; depolymerization; financial economics; fish; fish farms; fluorescent antibody technique; freshwater; microfilaments; quantitative polymerase chain reaction; viruses; China
Abstract:
... BACKGROUND: Infectious spleen and kidney necrosis virus (ISKNV) belongs to the genus Megalocytivirus from the family Iridoviridae. Megalocytivirus causes severe economic losses to tropical freshwater and marine culture industry in Asian countries and is devastating to the mandarin fish farm industry in China particularly. METHODS: We investigated the involvement of microfilaments in the early and ...
DOI:
10.1186/1743-422X-10-77
PubMed:
23497248
PubMed Central:
PMC3599308

27. Cloning, characterisation, and expression analysis of the cathepsin D gene from rock bream (Oplegnathus fasciatus)

Author:
Kwang-Min Choi; Sang Hee Shim; Cheul-Min An; Bo-Hye Nam; Young-Ok Kim; Ju-Won Kim; Chan-il Park
Source:
Fish & shellfish immunology 2014 v.40 no.1 pp. 253-258
ISSN:
1050-4648
Subject:
phylogeny; cathepsin D; Streptococcus iniae; Miichthys miiuy; bream; open reading frames; gene expression; amino acids; Infectious spleen and kidney necrosis virus; spleen; active sites; homeostasis; Edwardsiella tarda; tissues; pathogens; messenger RNA; complementary DNA; muscles; Oplegnathus fasciatus; cDNA libraries; kidneys; papain; liver; innate immunity; glycosylation; expressed sequence tags
Abstract:
... Cathepsins are lysosomal cysteine proteases belonging to the papain family, members of which play important roles in normal metabolism for the maintenance of cellular homeostasis. Rock bream (Oplegnathus fasciatus) cathepsin D (RbCTSD) cDNAs were identified by expressed sequence tag analysis of a lipopolysaccharide-stimulated rock bream liver cDNA library. The full-length RbCTSD cDNA (1644 bp) con ...
DOI:
10.1016/j.fsi.2014.07.013

28. Detection and molecular characterization of infectious spleen and kidney necrosis virus from major ornamental fish breeding states in Peninsular Malaysia

Author:
K Subramaniam; M Shariff; A R Omar; M Hair‐Bejo; B L Ong
Source:
Journal of fish diseases 2014 v.37 no.7 pp. 609-618
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; breeding; coat proteins; enzymes; freshwater; genes; genotype; nucleotide sequences; ornamental fish; phylogeny; polymerase chain reaction; sequence analysis; turbot; viruses; Malaysia
Abstract:
... ‘Gold standard’ OIE reference PCR assay was utilized to detect the presence of infectious spleen and kidney necrosis virus (ISKNV) in freshwater ornamental fish from Malaysia. From total of 210 ornamental fish samples representing 14 species, ISKNV was detected in 36 samples representing 5 fish species. All positive cases did not show any clinical signs of ISKNV. Three restriction enzymes analyses ...
DOI:
10.1111/jfd.12152
PubMed:
23952914

29. Disease resistance and immune‐relevant gene expression in golden mandarin fish, Siniperca scherzeri Steindachner, infected with infectious spleen and kidney necrosis virus‐like agent

Author:
G W Shin; S L White; H U Dahms; H D Jeong; J H Kim
Source:
Journal of fish diseases 2014 v.37 no.12 pp. 1041-1054
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; Siniperca scherzeri; disease resistance; financial economics; fish; gene expression; genes; interleukin-8; kidneys; messenger RNA; mortality; pathogenicity; quantitative polymerase chain reaction; spleen
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV), family Iridoviridae, genus Megalocytivirus, may cause high mortality rates such as those seen in mandarin fish, Siniperca chuatsi. ISKNV has attracted much attention due to the possible environmental threat and economic losses it poses on both cultured and wild populations. We have investigated the pathogenicity of ISKNV‐like agent Megalocytivir ...
DOI:
10.1111/jfd.12182

30. ORF005L from infectious spleen and kidney necrosis virus is located in the inner mitochondrial membrane and induces apoptosis

Author:
Rui Wang; Yang Yi; Lihong Liu; Yanghong Lu; Shaoping Weng; Jianguo He; Xiaopeng Xu
Source:
Virus genes 2014 v.49 no.2 pp. 269-277
ISSN:
0920-8569
Subject:
Infectious spleen and kidney necrosis virus; Pimephales promelas; Western blotting; active sites; apoptosis; bioinformatics; double-stranded RNA; etiological agents; fish; genes; membrane potential; mitochondria; mitochondrial membrane; pandemic; pathogenesis; viruses
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) belongs to the genus Megalocytivirus in the family Iridoviridae. This virus is the etiological agent of a serious and pandemic disease in fish. Cells infected with ISKNV undergo apoptosis. In this study, the ISKNV ORF005L gene was characterized and functionally investigated. Bioinformatics analysis revealed that the ORF005L protein contains a reg ...
DOI:
10.1007/s11262-014-1088-2
PubMed:
24862228

31. Protective immunity against infectious spleen and kidney necrosis virus induced by immunization with DNA plasmid containing mcp gene in Chinese perch Siniperca chuatsi

Author:
Xiaozhe Fu; Ningqiu Li; Qiang Lin; Huizhi Guo; Defeng Zhang; Lihui Liu; Shuqin Wu
Source:
Fish & shellfish immunology 2014 v.40 no.1 pp. 259-266
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; Western blotting; adjuvants; coat proteins; financial economics; fish; gene expression regulation; genes; immune response; immunization; immunoglobulin M; interferon regulatory factor-7; intramuscular injection; mortality; muscle tissues; plasmids; quantitative polymerase chain reaction; recombinant vaccines; reverse transcriptase polymerase chain reaction; viral load
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) is the causative agent of a disease leading to high mortality and economic losses in Chinese perch, Siniperca chuatsi. There is an urgent need to develop an effective vaccine against this fatal disease. In this study, the mcp gene encoding the major capsid protein, the predominant structural component of the iridovirus particles, was cloned into ...
DOI:
10.1016/j.fsi.2014.07.012

32. Detection of dwarf gourami iridovirus (Infectious spleen and kidney necrosis virus) in populations of ornamental fish prior to and after importation into Australia, with the first evidence of infection in domestically farmed Platy (Xiphophorus maculatus)

Author:
Anneke E. Rimmer; Joy A. Becker; Alison Tweedie; Mark Lintermans; Matthew Landos; Fran Stephens; Richard J. Whittington
Source:
Preventive veterinary medicine 2015 v.122 no.1-2 pp. 181-194
ISSN:
0167-5877
Subject:
Infectious spleen and kidney necrosis virus; Xiphophorus maculatus; biosecurity; breeding; certification; imports; international trade; issues and policy; ornamental fish; pathogens; quantitative polymerase chain reaction; quarantine; risk; risk analysis; virulent strains; viruses; Queensland
Abstract:
... The movement of ornamental fish through international trade is a major factor for the transboundary spread of pathogens. In Australia, ornamental fish which may carry dwarf gourami iridovirus (DGIV), a strain of Infectious spleen and kidney necrosis virus (ISKNV), have been identified as a biosecurity risk despite relatively stringent import quarantine measures being applied. In order to gain know ...
DOI:
10.1016/j.prevetmed.2015.09.008

33. Early protein ORF086 is an effective vaccine candidate for infectious spleen and kidney necrosis virus in mandarin fish Siniperca chuatsi

Author:
Xiaozhe Fu; Ningqiu Li; Qiang Lin; Huizhi Guo; Lihui Liu; Zhibin Huang; Shuqin Wu
Source:
Fish & shellfish immunology 2015 v.46 no.2 pp. 200-205
ISSN:
1050-4648
Subject:
Escherichia coli; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; Western blotting; adjuvants; antigens; aquaculture industry; fish; gene expression regulation; genes; immunoglobulin M; interferon regulatory factor-7; mortality; muscle tissues; polyclonal antibodies; rabbits; transcription (genetics); vaccination; vaccines
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) has caused significant loss in the Mandarin fish (Siniperca chuatsi) aquaculture industry. Vaccination is an important measure to prevent fatal ISKNV infection. In this study, the ORF086 gene encoding an early protein helicase of ISKNV was cloned into the prokaryotic pET32a (+) and eukaryotic pcDNA3.1 (+) expression vectors and designated as pET0 ...
DOI:
10.1016/j.fsi.2015.05.052

34. First molecular characterisation and expression analysis of a teleost thioredoxin-interacting protein (TXNIP) gene from rock bream (Oplegnathus fasciatus)

Author:
Ju-Won Kim; Jung-Ho Lee; Jin-Sol Bae; Cheul Min An; Bo-Hye Nam; Ji-Min Jeong; Chan-il Park
Source:
Developmental and comparative immunology 2015 v.52 no.1 pp. 69-74
ISSN:
0145-305X
Subject:
Edwardsiella tarda; Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Streptococcus iniae; Takifugu rubripes; amino acids; bream; complementary DNA; erythrocytes; gene expression; genes; glucose; intestines; kidneys; messenger RNA; metabolism; open reading frames; phylogeny; spleen
Abstract:
... Thioredoxin-interacting protein (TXNIP) is an important regulator of glucose metabolism that functions by inhibiting cellular glucose uptake. The full-length rock bream (Oplegnathus fasciatus) TXNIP (RbTXNIP) cDNA (2499 bp) contains an open reading frame of 1188 bp encoding 396 amino acids. Furthermore, multiple alignments showed that the arrestin domain was well conserved among the other TXNIP se ...
DOI:
10.1016/j.dci.2015.04.012

35. Molecular cloning and expression analysis of an apoptosis-associated gene Daxx from zebrafish, Danio rerio

Author:
Lin Qi; Zhiming Xiang
Source:
Fish & shellfish immunology 2015 v.45 no.1 pp. 59-66
ISSN:
1050-4648
Subject:
Danio rerio; Infectious spleen and kidney necrosis virus; amino acids; apoptosis; bioinformatics; chromatin; embryogenesis; fluorescence microscopy; gene overexpression; innate immunity; luciferase; messenger RNA; molecular cloning; open reading frames; quantitative polymerase chain reaction; reporter genes; repressor proteins; tissues; transcription (genetics)
Abstract:
... The death domain-associated protein Daxx exerts many functions including the induction and inhibition of apoptosis, regulation of chromatin remodeling and gene transcription. In this report, we have cloned and characterized a Daxx ortholog from the zebrafish, Danio rerio. The bioinformatics analysis results indicated that the open reading frame (ORF) of zebrafish Daxx is 2,151bp long and encodes a ...
DOI:
10.1016/j.fsi.2015.03.040

36. Protein encoded by ORF093 is an effective vaccine candidate for infectious spleen and kidney necrosis virus in Chinese perch Siniperca chuatsi

Author:
Ningqiu Li; Xiaozhe Fu; Huizhi Guo; Qiang Lin; Lihui Liu; Defeng Zhang; Xiang Fang; Shuqin Wu
Source:
Fish & shellfish immunology 2015 v.42 no.1 pp. 88-90
ISSN:
1050-4648
Subject:
Escherichia coli; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; Western blotting; financial economics; fish; genes; mortality; muscle tissues; polyclonal antibodies; rabbits; recombinant proteins; vaccines; China
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) is the causative agent of a disease causing high mortality and economic losses in Chinese perch, Siniperca chuatsi in China. Little information about the antigenicity of ISKNV proteins is available. In this study the ORF093 gene of ISKNV was cloned into the prokaryotic expression vector pET32a(+) and eukaryotic expression vector pcDNA3.1(+), and ...
DOI:
10.1016/j.fsi.2014.10.008

37. Transcriptomic analysis of Mandarin fish brain cells infected with infectious spleen and kidney necrosis virus with an emphasis on retinoic acid-inducible gene 1-like receptors and apoptosis pathways

Author:
Xianqin Hu; Xiaozhe Fu; Ningqiu Li; Xingxing Dong; Lijuan Zhao; Jiangfeng Lan; Wei Ji; Weidong Zhou; Taoshan Ai; Shuqin Wu; Li Lin
Source:
Fish & shellfish immunology 2015 v.45 no.2 pp. 619-629
ISSN:
1050-4648
Subject:
4',6-diamidino-2-phenylindole; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; apoptosis; brain; databases; financial economics; fish; gene expression; gene expression regulation; industry; interleukin-1; pathogenesis; quantitative polymerase chain reaction; receptors; sequence analysis; staining; transcription factor NF-kappa B; transcriptomics; tumor necrosis factors; unigenes
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) has caused significant economic losses in the cultured Mandarin fish (Siniperca chuatsi) industry. The molecular mechanisms that underlie the pathogenesis of the viral infection remain poorly understood. In this study, deep RNA sequencing technique was used to analyze the transcriptomic profiles of Mandarin fish brain cells (CPB) at progressive t ...
DOI:
10.1016/j.fsi.2015.05.007

38. Detection of natural infection of infectious spleen and kidney necrosis virus in farmed tilapia by hydroxynapthol blue‐loop‐mediated isothermal amplification assay

Author:
R. Suebsing; P.J. Pradeep; S. Jitrakorn; S. Sirithammajak; J. Kampeera; W.A. Turner; V. Saksmerprome; B. Withyachumnarnkul; W. Kiatpathomchai
Source:
Journal of applied microbiology 2016 v.121 no.1 pp. 55-67
ISSN:
1364-5072
Subject:
Infectious spleen and kidney necrosis virus; Oreochromis niloticus; aquaculture industry; brain; coat proteins; color; colorimetry; control methods; experts; farm management; farmed fish; genes; hatcheries; loop-mediated isothermal amplification; microbial detection; oocytes; ovarian follicles; pathogens; polymerase chain reaction; screening; tissue tropism; viruses
Abstract:
... AIMS: Infectious spleen and kidney necrosis virus (ISKNV) has recently been recognized as a causative agent of serious systemic disease in tilapia. Our objective was to establish a new colorimetric loop‐mediated isothermal amplification (LAMP) assay with pre‐addition of hydroxynapthol blue (blue‐LAMP) to investigate ISKNV transmission in tilapia. METHODS AND RESULTS: The blue‐LAMP, targeting a maj ...
DOI:
10.1111/jam.13165

39. Development of red sea bream iridovirus concentration method in seawater by iron flocculation

Author:
Yasuhiko Kawato; Takafumi Ito; Takashi Kamaishi; Atushi Fujiwara; Mitsuru Ototake; Toshihiro Nakai; Kazuhiro Nakajima
Source:
Aquaculture 2016 v.450 pp. 308-312
ISSN:
0044-8486
Subject:
DNA; Infectious spleen and kidney necrosis virus; Seriola quinqueradiata; aquaculture; detection limit; fish diseases; flocculation; genome; monitoring; mortality; pathogens; prediction; quantitative analysis; quantitative polymerase chain reaction; rearing; seawater; viruses; Japan
Abstract:
... Pathogen dynamics in environmental water are considered to be highly associated with the prevalence of fish disease. To examine the dynamics of red sea bream iridovirus (RSIV), which has caused serious damage in marine aquacultures in Japan, we developed a highly sensitive and quantitative method by combining the viral concentration and real-time PCR to detect the virus in seawater. The iron flocc ...
DOI:
10.1016/j.aquaculture.2015.08.016

40. Differential autophagic effects triggered by five different vertebrate iridoviruses in a common, highly permissive mandarinfish fry (MFF-1) cell model

Author:
Hemei Qi; Yang Yi; Shaoping Weng; Weibing Zou; Jianguo He; Chuanfu Dong
Source:
Fish & shellfish immunology 2016 v.49 pp. 407-419
ISSN:
1050-4648
Subject:
Frog virus 3; Infectious spleen and kidney necrosis virus; Iridovirus; Santee-Cooper ranavirus; Western blotting; autophagy; fluorescence; host-pathogen relationships; models; salamanders and newts; transmission electron microscopy
Abstract:
... Autophagy of five vertebrate iridoviruses, including one megalocytivirus (infectious spleen and kidney necrosis virus, ISKNV) and four ranaviruses (Chinese giant salamander iridovirus, CGSIV; Tiger frog virus, TFV; Grouper iridovirus, GIV; and Largemouth bass virus, LMBV) were investigated in a common, highly permissive mandarinfish fry (MFF-1) cell model. The results showed marked autophagosome f ...
DOI:
10.1016/j.fsi.2015.12.041

41. Display of ISKNV orf086 protein on the surface of Aeromonas hydrophila and its immunogenicity in Chinese perch (Siniperca chuatsi)

Author:
Xiaozhe Fu; Qiang Lin; Lihui Liu; Hongru Liang; Zhibin Huang; Ningqiu Li
Source:
Fish & shellfish immunology 2016 v.56 pp. 286-293
ISSN:
1050-4648
Subject:
Aeromonas hydrophila; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; Western blotting; adjuvants; antibodies; aquaculture industry; bacteria; enzyme-linked immunosorbent assay; fish; fluorescent antibody technique; formalin; gene expression regulation; genes; homologous recombination; immune response; immunoglobulin M; interferon regulatory factor-7; lymphocyte proliferation; lysozyme; mixed infection; mortality; mutants; reverse transcriptase polymerase chain reaction; surface proteins; transcription (genetics); vaccines; viral antigens
Abstract:
... Co-infection with infectious spleen and kidney necrosis virus (ISKNV) and Aeromonas hydrophila is becoming ever more widespread in Chinese perch (Siniperca chuatsi) aquaculture industry, so that it’s necessary to develop the combined vaccine against ISKNV and A. hydrophila disease. The surface display of heterologous on bacteria using anchoring motifs from outer membranes proteins has already been ...
DOI:
10.1016/j.fsi.2016.07.023

42. First description of programmed cell death10 (PDCD10) in rock bream (Oplegnathus fasciatus): Potential relations to the regulation of apoptosis by several pathogens

Author:
Ju-Won Kim; Ji-Min Jeong; Jin-Sol Bae; Dong-Hee Cho; Sung Hee Jung; Jee-Youn Hwang; Mun-Gyeong Kwon; Jung Soo Seo; Gun-Wook Baeck; Chan-Il Park
Source:
Developmental and comparative immunology 2016 v.55 pp. 51-55
ISSN:
0145-305X
Subject:
Edwardsiella tarda; Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Streptococcus iniae; amino acids; apoptosis; bream; complementary DNA; erythrocytes; gene expression; gills; immune response; intestines; kidneys; liver; mortality; open reading frames; pathogens; spleen
Abstract:
... In this study, we isolated and characterized programmed cell death10 (PDCD10), which is known to be related to apoptosis, from rock bream (Oplegnathus fasciatus). The full-length rock bream PDCD10 (RbPDCD10) cDNA (1459 bp) contains an open reading frame of 633 bp that encodes 210 amino acids. Furthermore, multiple alignments revealed that the six of the α-helix bundles were well conserved among th ...
DOI:
10.1016/j.dci.2015.10.009

43. Molecular characterization and expression pattern of tumor suppressor protein p53 in mandarin fish, Siniperca chuatsi following virus challenge

Author:
Huizhi Guo; Xiaozhe Fu; Ningqiu Li; Qiang Lin; Lihui Liu; Shuqin Wu
Source:
Fish & shellfish immunology 2016 v.51 pp. 392-400
ISSN:
1050-4648
Subject:
Escherichia coli; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; brain; complementary DNA; fish; gene expression; gene expression regulation; immune response; kidneys; messenger RNA; polyclonal antibodies; protein synthesis; quantitative polymerase chain reaction; rabbits; spleen; tissues; tumor suppressor protein p53; viruses
Abstract:
... In recent years, the tumor suppressor protein p53, which is crucial for cellular defense against tumor development, has also been implicated in host antiviral defense. In the present study, a 1555 bp full-length cDNA of p53 from mandarin fish (Siniperca chuatsi) (Sc-p53) was cloned and characterized. Quantitative real-time PCR assays revealed that Sc-p53 was expressed in all tissues examined, and ...
DOI:
10.1016/j.fsi.2016.03.003

44. A member of the immunoglobulin superfamily, orange-spotted grouper novel immune gene EcVig, is induced by immune stimulants and type I interferon

Author:
Ying-Chun Yeh; Ting-Yu Wang; Hsin-Yiu Chou; Han-You Lin; Tzong-Yueh Chen; Takashi Aoki; Han-Ching Wang
Source:
Fish & shellfish immunology 2016 v.58 pp. 415-422
ISSN:
1050-4648
Subject:
Betanodavirus; Epinephelus coioides; Infectious spleen and kidney necrosis virus; Iridovirus; antiviral properties; double-stranded RNA; genes; grouper; immune response; immunoglobulins; interferons; intestines; lipopolysaccharides; pathogen-associated molecular patterns
Abstract:
... A novel grouper immune gene, EcVig was identified in orange-spotted grouper (Epinephelus coioides). We recently determined that EcVig expression can be induced by infection with nervous necrosis virus (NNV, an RNA virus), whereas NNV replication may be suppressed when EcVig was overexpressed. Although EcVig appeared to be involved in grouper antiviral activity, its immune effects have not been wel ...
DOI:
10.1016/j.fsi.2016.09.039

45. Application and development of a TaqMan real-time PCR for detecting infectious spleen and kidney necrosis virus in Siniperca chuatsi

Author:
Qiang Lin; Xiaozhe Fu; Lihui Liu; Hongru Liang; Huizhi Guo; Shuwen Yin; Venkatesh Kumaresan; Zhibin Huang; Ningqiu Li
Source:
Microbial pathogenesis 2017 v.107 pp. 98-105
ISSN:
0882-4010
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; financial economics; genes; monitoring; quantitative polymerase chain reaction; spleen; tissues
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) is one of the major epidemiological agents that had caused great economic loss in Chinese perch (Siniperca chuatsi). In this study, a specific TaqMan real-time PCR was developed using a pair of primers and a TaqMan probe specific to the ORF007 gene of ISKNV to rapidly detect ISKNV copies in Chinese perch samples. This assay was optimized to produ ...
DOI:
10.1016/j.micpath.2017.02.046

46. Autophagy promoted infectious kidney and spleen necrosis virus replication and decreased infectious virus yields in CPB cell line

Author:
Chen Li; Xiaozhe Fu; Qiang Lin; Lihui Liu; Hongru Liang; Zhibin Huang; Ningqiu Li
Source:
Fish & shellfish immunology 2017 v.60 pp. 25-32
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; autophagy; chloroquine; cytoplasm; industry; pathogenesis; proteins; virus replication; viruses
Abstract:
... Autophagy plays important functions in viral replication and pathogenesis. In this study, we investigated the role of autophagy in the replication of infectious kidney and spleen necrosis virus (ISKNV), an agent that has caused devastating losses in Chinese perch (Siniperca chuatsi) industry. We found that ISKNV infection triggered the complete autophagic process, as demonstrated by microtubule-as ...
DOI:
10.1016/j.fsi.2016.11.037

47. Co-infections of infectious spleen and kidney necrosis virus and Siniperca chuatsi rhabdovirus in Chinese perch (Siniperca chuatsi)

Author:
Qiang Lin; Xiaozhe Fu; Ningqiu Li; Quanyuan Wan; Wenjie Chen; Yunmao Huang; Zhibin Huang; Jun Li; Lijuan Zhao; Li Lin
Source:
Microbial pathogenesis 2017 v.111 pp. 422-430
ISSN:
0882-4010
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; brain; farmed fish; mixed infection; pathogens; tissues; vaccines; viral load; viruses
Abstract:
... In spite of the quite common co-infections of viruses in the cultured fish, most of the previous studies have just simply focused on the infection of a single pathogen. In this report, we observed that about 13% of cultured Chinese perch have been co-infected by infectious spleen and kidney necrosis virus (ISKNV) and Siniperca chuatsi rhabdovirus (SCRV). Furthermore, Chinese perch could co-infecte ...
DOI:
10.1016/j.micpath.2017.09.002

48. Development of a highly permissive cell line from spotted knifejaw (Oplegnathus punctatus) for red sea bream iridovirus

Author:
Yasuhiko Kawato; Hirofumi Yamashita; Kei Yuasa; Satoshi Miwa; Kazuhiro Nakajima
Source:
Aquaculture 2017 v.473 pp. 291-298
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; Oplegnathus; cytopathogenicity; mortality; vaccines; viral load
Abstract:
... We developed a cell line (SKF-9) from spotted knifejaw (Oplegnathus punctatus), which is highly susceptible to red sea bream iridovirus (RSIV). Cytopathic effect (CPE) characterized by rounding and enlargement was clearly observed in RSIV-infected SKF-9 cells. The amount of viral production of SKF-9 cells was approximately 100 times greater than that of GF cells, which is conventionally used for R ...
DOI:
10.1016/j.aquaculture.2017.02.027

49. High reduced/oxidized glutathione ratio in infectious spleen and kidney necrosis virus-infected cells contributes to degradation of VP08R multimers

Author:
Jian-Hui He; Muting Yan; Hongliang Zuo; Shengwen Niu; Jia Yuan; Shao-Ping Weng; Jianguo He; Xiaopeng Xu
Source:
Veterinary microbiology 2017 v.207 pp. 19-24
ISSN:
0378-1135
Subject:
Infectious spleen and kidney necrosis virus; NADP (coenzyme); basement membrane; crosslinking; disulfide bonds; endothelial cells; enzymes; fish; glutathione; kidneys; necrosis; spleen; viral proteins; virion; viruses
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) is the type species of the genus Megalocytivirus, family Iridoviridae. The ISKNV-infected cells in fish tissues are attached by lymphatic endothelial cells (LECs), which is a unique pathological phenomenon of ISKNV infection. The viral proteins VP23R and VP08R and the host protein nidogen-1 constitute the virus-mock basement membrane (VMBM) on th ...
DOI:
10.1016/j.vetmic.2017.05.024

50. Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major)

Author:
Eitaro Sawayama; Shiho Tanizawa; Shin-Ichi Kitamura; Kei Nakayama; Kohei Ohta; Akiyuki Ozaki; Motohiro Takagi
Source:
Marine biotechnology 2017 v.19 no.6 pp. 601-613
ISSN:
1436-2228
Subject:
Infectious spleen and kidney necrosis virus; Pagrus major; chromosome mapping; disease resistance; females; genes; genetic markers; high-throughput nucleotide sequencing; linkage groups; males; marker-assisted selection; microsatellite repeats; phenotype; phenotypic variation; quantitative trait loci; Japan
Abstract:
... Red sea bream iridoviral disease (RSIVD) is a major viral disease in red sea bream farming in Japan. Previously, we identified one candidate male individual of red sea bream that was significantly associated with convalescent individuals after RSIVD. The purpose of this study is to identify the quantitative trait loci (QTL) linked to the RSIVD-resistant trait for future marker-assisted selection ( ...
DOI:
10.1007/s10126-017-9779-z

51. Infectious spleen and kidney necrosis disease (ISKND) outbreaks in farmed barramundi (Lates calcarifer) in Vietnam

Author:
H.T. Dong; S. Jitrakorn; P. Kayansamruaj; N. Pirarat; C. Rodkhum; T. Rattanarojpong; S. Senapin; V. Saksmerprome
Source:
Fish & shellfish immunology 2017 v.68 pp. 65-73
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Lates calcarifer; amino acid sequences; brain; coat proteins; disease prevention; epitopes; farmers; farms; genes; genotype; histology; in situ hybridization; kidneys; liver; marine fish; models; necrosis; nucleotide sequences; pancreas; polymerase chain reaction; prediction; sequence analysis; spleen; vaccines; viruses; Vietnam
Abstract:
... Emergence of a disease with clinical signs resembling megalocytivirus infection seriously affected large-scale barramundi farms in Vietnam in 2012–2014 with estimated losses reaching $435,810 per year. An oil-based, inactivated vaccine against red sea bream iridovirus (RSIV) was applied in one farm for disease prevention without analysis of the causative agent, and the farmer reported inadequate p ...
DOI:
10.1016/j.fsi.2017.06.054

52. Nile tilapia reared under full-strength seawater: Hemato-immunological changes and susceptibility to pathogens

Author:
Biboon Withyachumnarnkul; Iyapa Palang; Jareeporn Reungsri; Sarawut Sirithammajak; Sarocha Jitrakorn; Wansika Kiatpathomchai; Vanvimon Saksmerprome; Pattira Pongtippatee; Boonsirm Withyachumnarnkul
Source:
Aquaculture 2017 v.480 pp. 42-50
ISSN:
0044-8486
Subject:
DNA; Francisella; Infectious spleen and kidney necrosis virus; Oreochromis niloticus; Streptococcus agalactiae; blood platelets; blood serum; brain; coculture; digoxigenin; fish; freshwater; gills; gonads; hematocrit; in situ hybridization; industry; kidneys; leukocyte count; liver; lymphocytes; lysozyme; mortality; necrotizing hepatopancreatitis; pathogens; polymerase chain reaction; rearing; salinity; seawater; shrimp; shrimp culture; spleen; viruses
Abstract:
... Co-culture of Nile tilapia, Oreochromis niloticus, with marine shrimp has been an increasing farm practice as evidence has suggested that the co-culture is one of a few ways to mitigate shrimp diseases, especially the acute hepatopancreatic necrosis disease that has affected shrimp culture industry worldwide. Since Nile tilapia are basically freshwater species, having them in co-culture with marin ...
DOI:
10.1016/j.aquaculture.2017.08.002

53. Susceptibility of a number of Australian freshwater fishes to dwarf gourami iridovirus (Infectious spleen and kidney necrosis virus)

Author:
A E Rimmer; R J Whittington; A Tweedie; J A Becker
Source:
Journal of fish diseases 2017 v.40 no.3 pp. 293-310
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Maccullochella peelii peelii; Macquaria ambigua; Macquaria australasica; Tandanus tandanus; aquaculture; catfish; fish diseases; freshwater; freshwater fish; inclusion bodies; international trade; juveniles; mortality; perch; quantitative polymerase chain reaction; risk; virus replication; viruses; Australia; Europe; South East Asia
Abstract:
... Megalocytiviruses cause high mortality diseases that have seriously impacted aquaculture, with the most frequent outbreaks occurring in East and South‐East Asia. The international trade of juvenile fish for food and ornamental aquaculture has aided the spread of these viruses, which have spread to Europe and Australia and other regions. Australian freshwater fishes were examined for susceptibility ...
DOI:
10.1111/jfd.12510

54. TC38, a teleost TFPI-2 peptide that kills bacteria via penetration of the cell membrane and interaction with nucleic acids

Author:
Min Zhang; Bin Yue; Ai-hua Zhang; Guang-hua Wang; Yong Liu; Shun Zhou; Shun-feng Cheng; Ning-qiu Li
Source:
Fish & shellfish immunology 2017 v.64 pp. 104-110
ISSN:
1050-4648
Subject:
Cynoglossus semilaevis; DNA; Gram-negative bacteria; Infectious spleen and kidney necrosis virus; Micrococcus luteus; RNA; Vibrio ichthyoenteri; Vibrio parahaemolyticus; Vibrio vulnificus; anti-infective agents; antibacterial properties; aquaculture; blood coagulation; cell death; cell membranes; fish; humans; mechanism of action; peptides
Abstract:
... Tissue factor pathway inhibitor 2 (TFPI-2) is an analog of TFPI-1 and a potent endogenous inhibitor of tissue factor (TF)-mediated blood coagulation. Recent reports have proven that the C-terminal of TFPI-2 peptides in humans and several other vertebrates possesses antibacterial activity against Gram-positive and Gram-negative bacteria. In our previous study, we reported that the TFPI-2 peptide, T ...
DOI:
10.1016/j.fsi.2017.03.001

55. The core ubiquitin system of mandarin fish, Siniperca chuatsi, can be utilized by infectious spleen and kidney necrosis virus

Author:
Runqing Huang; Jing Zhang; Guohua Zhu; Jianguo He; Junfeng Xie
Source:
Fish & shellfish immunology 2017 v.70 pp. 293-301
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; Siniperca chuatsi; adaptive immunity; blood; fish fry; gene expression; gene expression regulation; genes; host-pathogen relationships; immune evasion; immune response; plant diseases and disorders; plant viruses; recombinant proteins; tissues; ubiquitin; ubiquitin-protein ligase; ubiquitination; viruses
Abstract:
... The process of ubiquitination regulates various cellular processes. The ubiquitin-proteasome system (UPS) in fish, which is important for the generation of innate and adaptive immune responses to pathogens, is the target of aquatic viruses to achieve immune evasion. We cloned and characterized three genes, namely, a ubiquitin-activating enzyme (ScE1), a ubiquitin-conjugating enzyme (ScE2), and a H ...
DOI:
10.1016/j.fsi.2017.09.017

56. The first report of cathepsin A gene in a teleost (rock bream, Oplegnathus fasciatus): An investigation of immune functions upon infection with several pathogens

Author:
Ju-Won Kim; Hee Jeong Kong; Bo-Hye Nam; Jung Youn Park; Do-Hyung Kim; Ji-Min Jeong; Chan-Il Park
Source:
Developmental and comparative immunology 2017 v.76 pp. 262-267
ISSN:
0145-305X
Subject:
Edwardsiella tarda; Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Streptococcus iniae; active sites; amino acid sequences; brain; bream; carboxypeptidase C; complementary DNA; eyes; genes; gills; heart; histidine; immune response; intestines; kidneys; leukocytes; liver; open reading frames; pathogens; serine; serine proteinases; spleen; stomach; tissues
Abstract:
... We isolated and characterised a cDNA encoding the lysosomal protective protein (serine protease) cathepsin A (CTSA) from rock bream (Oplegnathus fasciatus). The full-length rock bream CTSA (RbCTSA) cDNA (1814 bp) contains an open reading frame of 1419 bp that encodes 472 amino acids. Alignment of multiple CTSA protein sequences revealed that the active site serine and histidine residues were well- ...
DOI:
10.1016/j.dci.2017.06.004

57. Antibody profiling using a recombinant protein–based multiplex ELISA array accelerates recombinant vaccine development: Case study on red sea bream iridovirus as a reverse vaccinology model

Author:
Tomomasa Matsuyama; Natsumi Sano; Tomokazu Takano; Takamitsu Sakai; Motoshige Yasuike; Atushi Fujiwara; Yasuhiko Kawato; Jun Kurita; Kazunori Yoshida; Yukinori Shimada; Chihaya Nakayasu
Source:
Vaccine 2018 v.36 no.19 pp. 2643-2649
ISSN:
0264-410X
Subject:
Infectious spleen and kidney necrosis virus; Seriola dumerili; Seriola quinqueradiata; antibodies; antigens; blood serum; case studies; enzyme-linked immunosorbent assay; fish; genes; immune response; models; pathogens; prediction; protective effect; recombinant vaccines; screening; sorbents; vaccine development
Abstract:
... Predicting antigens that would be protective is crucial for the development of recombinant vaccine using genome based vaccine development, also known as reverse vaccinology. High-throughput antigen screening is effective for identifying vaccine target genes, particularly for pathogens for which minimal antigenicity data exist. Using red sea bream iridovirus (RSIV) as a research model, we developed ...
DOI:
10.1016/j.vaccine.2018.03.059

58. Black rockfish C-type lectin, SsCTL4: A pattern recognition receptor that promotes bactericidal activity and virus escape from host immune defense

Author:
Du Xue; Wang Guang-hua; Su Yan-li; Zhang Min; Hu Yong-hua
Source:
Fish & shellfish immunology 2018 v.79 pp. 340-350
ISSN:
1050-4648
Subject:
Edwardsiella tarda; Gram-positive bacteria; Infectious spleen and kidney necrosis virus; Sebastes melanops; Sebastes schlegelii; Vibrio anguillarum; antibacterial properties; bacterial infections; calcium; cysteine; fish; humans; immune response; immunologic receptors; lectins; macrophages; mannose; open reading frames; shellfish; tissues; viruses
Abstract:
... C-type lectin (CTL) is an immune receptor and is received extensive attention of its important roles in immune response and immune escape. Some CTL, such as CTL4, has been well characterized in human and several other mammals, but much less documentation exists about the immunological function of CTL4 in lower vertebrates. In the present study, a C-type lectin domain family 4 member, SsCTL4, which ...
DOI:
10.1016/j.fsi.2018.05.033

59. Complete genome sequence and phylogenetic analysis of megalocytivirus RSIV-Ku: A natural recombination infectious spleen and kidney necrosis virus

Author:
Jing-Yi Shiu; Jiann-Ruey Hong; Chen-Chun Ku; Chiu-Ming Wen
Source:
Archives of virology 2018 v.163 no.4 pp. 1037-1042
ISSN:
0304-8608
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; Pagrus major; adenosinetriphosphatase; coat proteins; genes; genotype; open reading frames; phylogeny; viruses
Abstract:
... Megalocytiviruses are classified into three genotypes, infectious spleen and kidney necrosis virus (ISKNV), red seabream virus (RSIV), and turbo reddish body iridovirus (TRBIV), based on the major capsid protein and ATPase genes. However, only a few complete genome sequences have been obtained. This paper reports the complete genome sequence and phylogenetic analysis of an RSIV-Ku strain megalocyt ...
DOI:
10.1007/s00705-017-3689-2

60. Composition and transcription of all interferon regulatory factors (IRFs), IRF1‒11 in a perciform fish, the mandarin fish, Siniperca chuatsi

Author:
Zubair Ahmed Laghari; Li Li; Shan Nan Chen; Hui Jun Huo; Bei Huang; Ying Zhou; P. Nie
Source:
Developmental and comparative immunology 2018 v.81 pp. 127-140
ISSN:
0145-305X
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; aquaculture; cytokines; fish; gene expression; genes; immune response; immunomodulation; interferon regulatory factors; interferons; kidneys; pathogens; spleen; tissues
Abstract:
... Interferon regulatory factors (IRFs) are a family of mediators in various biological processes including immune modulation of interferon (IFN) and proinflammatory cytokine expression. However, the data on the complete composition of IRFs is rather limited in teleost fish. In the present study, all IRF members, i.e. IRF1‒11 with two IRF4, IRF4a and IRF4b have been characterised in an aquaculture sp ...
DOI:
10.1016/j.dci.2017.11.015

61. First description of a natural infection with spleen and kidney necrosis virus in zebrafish

Author:
Roberto Bermúdez; Ana Paula Losada; Ana Manuela de Azevedo; Jorge Guerra‐Varela; David Pérez‐Fernández; Laura Sánchez; Francesc Padrós; Barbara Nowak; María Isabel Quiroga
Source:
Journal of fish diseases 2018 v.41 no.8 pp. 1283-1294
ISSN:
0140-7775
Subject:
Danio rerio; Infectious spleen and kidney necrosis virus; anorexia; biosecurity; distress; farmed fish; fins; gills; hemorrhage; histopathology; kidneys; models; morbidity; necrosis; pathogenesis; phylogeny; research facilities; spleen; swimming; tissues; viruses; Spain
Abstract:
... Zebrafish has become a popular research model in the last years, and several diseases affecting zebrafish research facilities have been reported. However, only one case of naturally occurring viral infections was described for this species. In 2015, infectious spleen and kidney necrosis virus (ISKNV) was detected in zebrafish from a research facility in Spain. Affected fish showed lethargy, loss o ...
DOI:
10.1111/jfd.12822

62. Functional, signalling and transcriptional differences of three distinct type I IFNs in a perciform fish, the mandarin fish Siniperca chuatsi

Author:
Zubair Ahmed Laghari; Shan Nan Chen; Li Li; Bei Huang; Zhen Gan; Ying Zhou; Hui Jun Huo; Jing Hou; Pin Nie
Source:
Developmental and comparative immunology 2018 v.84 pp. 94-108
ISSN:
0145-305X
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; cytokine receptors; fish; genes; nucleotide sequences; transcription (genetics)
Abstract:
... Teleost fish are unique in having type I and type II interferons (IFNs) only, and the type I IFNs are classified into Group one and Group two based on the presence of two or four cysteines respectively, and are further classified into seven subgroups. In the present study, three distinct type I IFNs, IFNc, IFNd and IFNh, have been identified in the genome sequences of a perciform fish, the mandari ...
DOI:
10.1016/j.dci.2018.02.008

63. Genotype determination of megalocytivirus from Indonesian Marine Fishes

Author:
MURWANTOKO; DINI WAHYU KARTIKA SARI; CHRISTINA RETNO HANDAYANI; RICHARD J. WHITTINGTON
Source:
Biodiversitas 2018 v.19 no.5 pp. 1730-1736
ISSN:
2085-4722
Subject:
Cromileptes altivelis; DNA; DNA-directed DNA polymerase; Epinephelus fuscoguttatus; Gasterosteus aculeatus; Infectious spleen and kidney necrosis virus; Iridovirus; Lates calcarifer; Mycteroperca tigris; Turbot reddish body iridovirus; adenosinetriphosphatase; coat proteins; ethanol; farmed fish; formalin; freshwater; genotype; genotyping; histology; hypertrophy; kidneys; liver; mortality; polymerase chain reaction; spleen; viruses; Indonesia
Abstract:
... Murwantoko, Sari DWK, Handayani CR, Whittington RJ. 2018. Genotype determination of megalocytivirus from Indonesian Marine Fishes. Biodiversitas 19: 1730-1736. Megalocytivirus is the newest genus within the family of Iridoviridae which can be divided into groups represented by red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis virus (ISKNV) and turbot reddish body iridovirus (T ...
DOI:
10.13057/biodiv/d190519

64. Molecular cloning, characterization and expression analysis of complement components in red sea bream (Pagrus major) after Edwardsiella tarda and red sea bream Iridovirus (RSIV) challenge

Author:
Mohammad Ali Noman Reza; Sipra Mohapatra; Sonoko Shimizu; Shin-Ichi Kitamura; Shogo Harakawa; Hidemasa Kawakami; Kei Nakayama; Eitaro Sawayama; Takahiro Matsubara; Kohei Ohta; Tapas Chakraborty
Source:
Fish & shellfish immunology 2018 v.82 pp. 286-295
ISSN:
1050-4648
Subject:
Edwardsiella tarda; Infectious spleen and kidney necrosis virus; Pagrus major; complement; fish; genes; immune response; lectins; liver; messenger RNA; molecular cloning; phylogeny; shellfish; tissue distribution; transcription (genetics)
Abstract:
... The complement system plays an important role in immune regulation and acts as the first line of defense against any pathogenic attack. To comprehend the red sea bream (Pagrus major) immune response, three complement genes, namely, pmC1r, pmMASP and pmC3, belonging to the classical, lectin and alternative complement cascade, respectively, were identified and characterized. pmC1r, pmMASP, and pmC3 ...
DOI:
10.1016/j.fsi.2018.08.027

65. TO17: A teleost antimicrobial peptide that induces degradation of bacterial nucleic acids and inhibits bacterial infection in red drum, Sciaenops ocellatus

Author:
Shu-wen He; Guang-hua Wang; Bin Yue; Shun Zhou; Min Zhang
Source:
Fish & shellfish immunology 2018 v.72 pp. 639-645
ISSN:
1050-4648
Subject:
DNA; Edwardsiella tarda; Infectious spleen and kidney necrosis virus; Micrococcus luteus; RNA; Sciaenops ocellatus; Staphylococcus aureus; Vibrio vulnificus; amino acids; anti-infective agents; antibacterial properties; antimicrobial peptides; aquaculture; bacterial infections; blood cells; blood coagulation; blood serum; cell membranes; cytoplasm; fish; hemolysis; immunostimulants; macrophages; microorganisms; nitric oxide; pathogens; shellfish; tissues
Abstract:
... Tissue factor pathway inhibitor (TFPI)-1 is well known for its role as an inhibitor of blood coagulation. Several studies have demonstrated that the C-terminal peptides of TFPI-1 are active against a broad spectrum of microorganisms. In a previous study, we found that TO17 (with 17 amino acids), a TFPI-1 C-terminal peptide from red drum (Sciaenops ocellatus), was active against Edwardsiella tarda. ...
DOI:
10.1016/j.fsi.2017.11.038

66. iTRAQ-based proteomic profile analysis of ISKNV-infected CPB cells with emphasizing on glucose metabolism, apoptosis and autophagy pathways

Author:
Shiwei Wu; Lujun Yu; Xiaozhe Fu; Xi Yan; Qiang Lin; Lihui Liu; Hongru Liang; Ningqiu Li
Source:
Fish & shellfish immunology 2018 v.79 pp. 102-111
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; Western blotting; apoptosis; autophagy; caspase-8; fish; fumarate hydratase; glucose; glucose 6-phosphate; hosts; industry; pathogenesis; peptides; phosphopyruvate hydratase; protein synthesis; pyruvate dehydrogenase (acetyl-transferring) kinase; pyruvate dehydrogenase (lipoamide); shellfish; tubulin
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) has caused significant losses in the cultured mandarin fish (Siniperca chuatsi) industry. The molecular mechanisms that underlie interaction between ISKNV and hosts are not fully understood. In this study, the proteomic profile of CPB cells at progressive time points after ISKNV infection was analyzed by isobaric tags for relative and absolute qu ...
DOI:
10.1016/j.fsi.2018.05.002

67. The infection of red seabream iridovirus in mandarin fish (Siniperca chuatsi) and the host immune related gene expression profiles

Author:
Xiaodan Liu; Nan Chen; Xiaojian Gao; Yingying Zhang; Xixi Li; Yue Zhang; Xuwen Bing; Hezhong Huang; Xiaojun Zhang
Source:
Fish & shellfish immunology 2018 v.74 pp. 474-484
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; Pagrus major; Siniperca chuatsi; financial economics; fish culture; genes; genotype; hemorrhage; histopathology; immune response; inflammation; interleukin-1beta; interleukin-8; kidneys; leukocytes; liver; marine fish; morbidity; mortality; necrosis; pathogenesis; pathogenicity; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; shellfish; signs and symptoms (animals and humans); spleen; stomach; tissues; transcriptional activation; tumor necrosis factor-alpha
Abstract:
... Red Sea bream iridovirus (RSIV) was initially isolated from marine fish, which belongs to Megalocytivirus, Iridoviridae. It can cause great economic losses in fish culture with high morbidity and mortality. In the present study, the pathogenicity and immune response associated with a RSIV genotype megalocytivirus infection were determined in mandarin fish (Siniperca chuatsi). Fish challenged showe ...
DOI:
10.1016/j.fsi.2018.01.020

68. A natural infection by the red sea bream iridovirus‐type Megalocytivirus in the golden mandarin fish Siniperca scherzeri

Author:
K I Kim; S D Hwang; M Y Cho; S H Jung; Y C Kim; H D Jeong
Source:
Journal of fish diseases 2018 v.41 no.8 pp. 1229-1233
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Pagrus major; Siniperca scherzeri; Turbot reddish body iridovirus; adenosinetriphosphatase; coat proteins; freshwater fish; genes; genetic variation; phylogeny; quantitative polymerase chain reaction; spleen; Japan; Korean Peninsula
Abstract:
... An outbreak of a Megalocytivirus infection was found in the golden mandarin fish Siniperca scherzeri during September and October 2016, in Korea. Phylogeny and genetic diversity based on the major capsid protein (MCP) and adenosine triphosphatase (ATPase) genes showed a new strain. Designated as GMIV, this strain derived from the golden mandarin fish was suggested to belong to the red sea bream ir ...
DOI:
10.1111/jfd.12815

69. A teleost TFPI-2 peptide that possesses a broad antibacterial spectrum and immune-stimulatory properties

Author:
Shu-wen He; Jing-jing Wang; Xue Du; Bin Yue; Guang-hua Wang; Shun Zhou; Bing Xie; Min Zhang
Source:
Fish & shellfish immunology 2018 v.82 pp. 469-475
ISSN:
1050-4648
Subject:
DNA; Gram-negative bacteria; Gram-positive bacteria; Infectious spleen and kidney necrosis virus; Micrococcus luteus; RNA; Sciaenops ocellatus; Staphylococcus aureus; Vibrio ichthyoenteri; Vibrio scophthalmi; Vibrio vulnificus; amino acids; anti-infective agents; antibacterial properties; antimicrobial peptides; aquaculture; bacterial infections; blood cells; blood coagulation; cell membranes; cytoplasm; fish; hemolysis; humans; immunology; macrophages; pathogens; shellfish; tissues
Abstract:
... Tissue factor pathway inhibitor 2 (TFPI-2) is an analogue of TFPI-1 and a potent endogenous inhibitor of tissue factor (TF)-mediated blood coagulation. Previous reports have shown that several peptides derived from human and vertebrates TFPI-2 possess antibacterial activity against diverse bacteria. In this study, a C-terminal peptide, TO24 (with 24 amino acids), derived from red drum (Sciaenops o ...
DOI:
10.1016/j.fsi.2018.08.051

70. Characterization of gene expression profiles and functional analysis of peptidoglycan recognition protein 2 from rock bream (Oplegnathus fasciatus)

Author:
Kwang-Min Choi; Min-Soo Joo; Dong Hee Cho; Jin-Sol Bae; Ji-Min Jung; Jee Youn Hwang; Mun-Gyeong Kwon; Jung Soo Seo; Seong Don Hwang; Bo-Yeong Jee; Do-Hyung Kim; Chan-Il Park
Source:
Fish & shellfish immunology 2019 v.84 pp. 1068-1074
ISSN:
1050-4648
Subject:
Edwardsiella piscicida; Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Streptococcus iniae; amino acids; antimicrobial properties; bacteria; bacterial growth; bacterial infections; bream; cell membranes; complementary DNA; enzymes; gene expression; high-throughput nucleotide sequencing; intestines; liver; open reading frames; peptidoglycans; phagocytosis; phylogeny; protein domains; zinc
Abstract:
... Peptidoglycan recognition protein 2 (PGRP2) is a Zn2+-dependent peptidase that plays important roles in binding to microbial components of the cell membrane, inducing phagocytosis and antimicrobial activity. Rock bream (Oplegnathus fasciatus) PGRP2 (RbPGRP2) was identified in the intestine by next generation sequencing (NGS) analysis. The open reading frame (ORF) the RbPGRP2 cDNA (470 amino acid r ...
DOI:
10.1016/j.fsi.2018.11.025

71. Chitosan and anisodamine improve the immune efficacy of inactivated infectious spleen and kidney necrosis virus vaccine in Siniperca chuatsi

Author:
Jiacheng Zhang; Xiaozhe Fu; Yanqi Zhang; Wentao Zhu; Yong Zhou; Gailing Yuan; Xiaoling Liu; Taoshan Ai; Lingbing Zeng; Jianguo Su
Source:
Fish & shellfish immunology 2019 v.89 pp. 52-60
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; acid phosphatase; blood serum; catalase; chitosan; fish; immune response; infectious diseases; liver; messenger RNA; mortality; protective effect; reverse transcriptase polymerase chain reaction; spleen; tissues; tumor necrosis factor-alpha; urea nitrogen; vaccination; vaccine adjuvants; vaccines; virus replication
Abstract:
... Siniperca chuatsi is an economically important fish in China, but infectious spleen and kidney necrosis virus (ISKNV) causes high mortality and significant economic losses. Currently, vaccination is the most promising strategy to prevent infectious diseases, while adjuvant can effectively enhance immune responses. In this study, inactivated ISKNV vaccine was prepared, then poly (I:C), chitosan, an ...
DOI:
10.1016/j.fsi.2019.03.040

72. Data on molecular characterisation and expression analysis of the interferon-related developmental regulator 2 (IFRD2) gene from red sea bream, Pagrus major

Author:
Kyung Ho Kim; Andre Kim; Chan-Il Park
Source:
Data in Brief 2019 v.25 pp. 104142
ISSN:
2352-3409
Subject:
Edwardsiella piscicida; Infectious spleen and kidney necrosis virus; Pagrus major; Streptococcus iniae; adults; amino acid sequences; brain; gastrulation; genes; heart; hematopoiesis; immune response; kidneys; liver; lungs; myoblasts; phylogeny; placenta; skeletal muscle
Abstract:
... The interferon-related developmental regulator 1 (IFRD1) protein is expected to play a role in the regulation of inflammatory responses in adult mice, since it is known to repress transcription of NF-κB in myoblasts that regenerate skeletal muscle after traumatic injury Micheli et al., 2011. The IFRD2 gene is expressed in many tissues including skeletal muscle, kidney, heart, brain, lung, placenta ...
DOI:
10.1016/j.dib.2019.104142

73. Data on molecular characterization and expression of the C-reactive protein (CRP) gene from rock bream, Oplegnathus fasciatus

Author:
Kyung Ho Kim; Do-Hyung Kim; Chan-Il Park
Source:
Data in Brief 2019 v.26 pp. 104506
ISSN:
2352-3409
Subject:
C-reactive protein; Edwardsiella piscicida; Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Streptococcus iniae; bream; gene expression; genes; inflammation; innate immunity; kidneys; nucleotide sequences; phylogeny; spleen
Abstract:
... C-reactive protein (CRP) is a member of the pentraxin family and is an acute-phase response to plasma protein; its level in blood increases rapidly in response to trauma, inflammation, and infection. In the present study, we analysed the molecular characteristics of the Oplegnathus fasciatus CRP (RbCRP) gene sequence using multiple alignments and phylogenetic analyses of the deduced amino acids. I ...
DOI:
10.1016/j.dib.2019.104506

74. Data on molecular characterization and expression profiles of the NF-κB repressing factor gene in red sea bream (Pagrus major)

Author:
Kwang-Min Choi; Jee Youn Hwang; Mun-Gyeong Kwon; Ji-Min Jeong; Jung Soo Seo; Seong Don Hwang; Bo-Yeong Jee; Mu-Chan Kim; Chan-Il Park
Source:
Data in Brief 2019 v.24 pp. 103977
ISSN:
2352-3409
Subject:
Infectious spleen and kidney necrosis virus; Pagrus major; Streptococcus iniae; amino acids; gene expression; genes; kidneys; liver; spleen; transcription (genetics); transcription factor NF-kappa B
Abstract:
... Nuclear factor-kappaB (NF-κB) repressing factor (NKRF) specifically inhibits the transcriptional activity of NF-κB protein. The PmNKRF cDNA is composed of 757 amino acid residues. Alignment analysis revealed that the G-patch and R3H domains are conserved in different organisms. We aimed to analyse red sea bream NKRF (PmNKRF) gene expression after infection with pathogens [Streptococcus iniae or re ...
DOI:
10.1016/j.dib.2019.103977

75. Data on molecular characterization and gene expression analysis of secretory carrier-associated membrane protein 5 (SCAMP5) from the red sea bream (Pagrus major)

Author:
Min Jin Heo; Andre Kim; Chan-Il Park
Source:
Data in Brief 2019 v.25 pp. 103901
ISSN:
2352-3409
Subject:
Edwardsiella piscicida; Infectious spleen and kidney necrosis virus; Pagrus major; Streptococcus iniae; cytokines; gene expression; genes; immune response; macrophages; membrane proteins; monocytes; phylogeny; secretion
Abstract:
... Secretory carrier membrane proteins (SCAMPs) are widely distributed integral membrane proteins implicated in membrane trafficking. Secretory carrier membrane protein 5 (SCAMP5) is expected to be involved in regulation of the immune response because it is expressed in a variety of immune tissues and promotes the secretion of cytokines in monocytes and macrophages. In this study, we performed an ana ...
DOI:
10.1016/j.dib.2019.103901

76. Deletion of the Infectious spleen and kidney necrosis virus ORF069L reduces virulence to mandarin fish Siniperca chuatsi

Author:
Yi-Fan Lin; Jian He; Ruo-Yun Zeng; Zhi-Min Li; Zhi-Yong Luo; Wei-Qiang Pan; Shao-Ping Weng; Chang-Jun Guo; Jian-Guo He
Source:
Fish & shellfish immunology 2019 v.95 pp. 328-335
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Retroviridae; Siniperca chuatsi; T-lymphocytes; amino acids; carcinogenesis; cell proliferation; death; fish; fish diseases; fruit diseases; gene deletion; genes; growth curves; homologous recombination; humans; immunoglobulin M; interleukin-1beta; interleukin-8; live vaccines; terminal repeat sequences; viral load; virulence; viruses; China
Abstract:
... Mandarin fish (Siniperca chuatsi) is a significant cultured species with high added value in China. With the expansion of farming, diseases of mandarin fish such as Infectious spleen and kidney necrosis virus (ISKNV) diseases are becoming more and more serious. Human endogenous retrovirus subfamily H long terminal repeat associating protein 2 (HHLA2) is a type 1 transmembrane molecule with three e ...
DOI:
10.1016/j.fsi.2019.10.039

77. First report of megalocytivirus (iridoviridae) in cultured bluegill sunfish, Lepomis macrochirus, in China

Author:
Lihui Liu; Lujun Yu; Xiaozhe Fu; Qiang Lin; Hongru Liang; Yinjie Niu; Ningqiu Li
Source:
Microbial pathogenesis 2019 v.135 pp. 103617
ISSN:
0882-4010
Subject:
Infectious spleen and kidney necrosis virus; Lepomis macrochirus; Siniperca chuatsi; aquaculture industry; brain; coat proteins; cytopathogenicity; electron microscopy; fish; genes; histopathology; intestines; liver; mortality; nucleotide sequences; pathogens; phylogeny; polymerase chain reaction; sequence alignment; spleen; taste; viral load; viruses; China
Abstract:
... The bluegill sunfish, Lepomis macrochirus, is an important aquacultural and recreational species in southern China because of its excellent taste, rapid growth rate, and good looks. At present, few pathogens are known to affect the bluegill sunfish. However, an iridovirus-like disease recently caused heavy losses to the bluegill sunfish aquaculture industry in Guangdong, China. We report that a vi ...
DOI:
10.1016/j.micpath.2019.103617

78. Functional characterization of IL-10 and its receptor subunits in a perciform fish, the mandarin fish, Siniperca chuatsi

Author:
Hui Jun Huo; Shan Nan Chen; Li Li; Pin Nie
Source:
Developmental and comparative immunology 2019 v.97 pp. 64-75
ISSN:
0145-305X
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; binding sites; cysteine; fish; gene expression; interleukin-10; interleukin-1beta; interleukin-6; interleukin-8; kidneys; mammals; receptors; signal transduction; spleen; splenocytes; tissues; tumor necrosis factor-alpha
Abstract:
... Interleukin (IL)-10 is an immune-regulatory cytokine with multiple functions. In the current study, IL-10 and its two receptors, IL-10R1 and IL-10R2 were identified in mandarin fish, Siniperca chuatsi. The inhibitory effect of mandarin fish IL-10 was investigated on pro-inflammatory cytokine expression and the ligand-receptor relationship. This IL-10 possesses conserved cysteine residues, predicte ...
DOI:
10.1016/j.dci.2019.03.017

79. Genetic diversity of Megalocytivirus from cultured fish in Korea

Author:
Kwang Il Kim; Eun Sun Lee; Jeong Wan Do; Seong Don Hwang; Miyoung Cho; Sung Hee Jung; Bo Young Jee; Woo Ju Kwon; Hyun Do Jeong
Source:
Aquaculture 2019 v.509 pp. 16-22
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; Lateolabrax japonicus; Platichthys stellatus; Siniperca scherzeri; Stephanolepis cirrhifer; Turbot reddish body iridovirus; bass; coat proteins; farmed fish; freshwater fish; genes; genetic variation; genotype; marine fish; mullet; sequence analysis; Korean Peninsula
Abstract:
... Since the first outbreak of Megalocytivirus infection in cultured marine fish in the 1990s, the Red sea bream iridovirus (RSIV) subtype II and Turbot reddish body iridovirus (TRBIV) type Megalocytivirus have been prevalent in Korea. Based on the major capsid protein (MCP) genes, the RSIV subtype II, as the predominant genotype, was identified in marine and freshwater fish including thread-sail fil ...
DOI:
10.1016/j.aquaculture.2019.05.014

80. Identification of infectious spleen and kidney necrosis virus from farmed barramundi Lates calcarifer in Thailand and study of its pathogenicity

Author:
Kittipong Thanasaksiri; Ryoko Takano; Kohei Fukuda; Tidaporn Chaweepack; Janenuj Wongtavatchai
Source:
Aquaculture 2019 v.500 pp. 188-191
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; Lates calcarifer; Pagrus major; coat proteins; disease control; dose response; emerging diseases; fluorescent antibody technique; genotype; kidneys; marine fish; monoclonal antibodies; mortality; necrosis; phylogeny; spleen; vaccines; virulence; Thailand
Abstract:
... Infectious spleen and kidney necrosis disease (ISKND) is one of major viral diseases in marine fish species. Here, we isolated infectious spleen and kidney necrosis virus (ISKNV) from diseased barramundi in Thailand and evaluated its pathogenicity with intramuscular challenge in naïve barramundi. ISKNV infection in grunt fin (GF) cells was observed within 14 days post inoculation (dpi) by immunofl ...
DOI:
10.1016/j.aquaculture.2018.09.035

81. Immersion vaccination of Mandarin fish Siniperca chuatsi against infectious spleen and kidney necrosis virus with a SWCNTs-based subunit vaccine

Author:
Zhao Zhao; Chen Zhang; Yi-Jun Jia; De-Kui Qiu; Qiang Lin; Ning-Qiu Li; Zhi-Bin Huang; Xiao-Zhe Fu; Gao-Xue Wang; Bin Zhu
Source:
Fish & shellfish immunology 2019 v.92 pp. 133-140
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; antibody formation; blood serum; carbon nanotubes; enzyme activity; fish; gastrointestinal system; genes; juveniles; mortality; protective effect; subunit vaccines; vaccination
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) cause a high mortality disease which lead to significant economic loss on mandarin fish in China. There is no effective drug or vaccine against this fatal disease at present. Meanwhile, many drugs and vaccines had no effect in many cases account of several impenetrable barriers (cell, skin and gastrointestinal tract). Here we reported an immersio ...
DOI:
10.1016/j.fsi.2019.06.001

82. Molecular cloning of Y-Box binding protein-1 from mandarin fish and its roles in stress-response and antiviral immunity

Author:
Jian He; Tao-Lin Xie; Xiao Li; Yang Yu; Zhi-Peng Zhan; Shao-Ping Weng; Chang-Jun Guo; Jian-Guo He
Source:
Fish & shellfish immunology 2019 v.93 pp. 406-415
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Rhabdoviridae; Siniperca chuatsi; amino acids; binding proteins; coat proteins; cold; cold shock proteins; cold stress; complementary DNA; enzymes; farmed fish; fish fry; gene expression regulation; gene overexpression; genes; innate immunity; luciferin; mammals; molecular cloning; open reading frames; plasmids; signal transduction; small interfering RNA; stress response; tissue distribution; tissues; transcription factor NF-kappa B; viruses
Abstract:
... Mandarin fish (Siniperca chuatsi) is a universally farmed fish species in China and has a large farming scale and economic value. With the high-density cultural mode in mandarin fish, viral diseases, such as infectious spleen and kidney necrosis virus (ISKNV) and Siniperca chuatsi rhabdovirus (SCRV), have increased loss, which has seriously restricted the development of aquaculture. Y-Box binding ...
DOI:
10.1016/j.fsi.2019.07.069

83. Stability of Infectious spleen and kidney necrosis virus and susceptibility to physical and chemical disinfectants

Author:
Cahya Fusianto; Paul M. Hick; Joy A. Becker
Source:
Aquaculture 2019 v.506 pp. 104-111
ISSN:
0044-8486
Subject:
DNA; Infectious spleen and kidney necrosis virus; Iridovirus; Maccullochella peelii peelii; Pagrus major; World Organization for Animal Health; aquaculture; aquariums; benzalkonium chloride; bioassays; biosecurity; cell culture; cellulose; certification; cost effectiveness; detection limit; disease control; disinfectants; disinfection; fetal bovine serum; fisheries; heat; host range; intraperitoneal injection; juveniles; ornamental fish; pH; pathogenicity; quantitative polymerase chain reaction; risk; signs and symptoms (animals and humans); sodium hypochlorite; tanks; Australia
Abstract:
... The genus Megalocytivirus (MCV) includes Infectious spleen and kidney necrosis virus (ISKNV) and red seabream iridovirus (RSIV) which are listed by the World Organization for Animal Health (OIE). MCV has a broad host range and can be present as a persistent subclinical infection in some fish. Australia is considered free from MCV and important aquaculture and wild fisheries are at risk. Consequent ...
DOI:
10.1016/j.aquaculture.2019.03.024

84. Viral infections in tilapines: More than just tilapia lake virus

Author:
Vimbai Irene Machimbirike; Mona Dverdal Jansen; Saengchan Senapin; Pongsak Khunrae; Triwit Rattanarojpong; Ha Thanh Dong
Source:
Aquaculture 2019 v.503 pp. 508-518
ISSN:
0044-8486
Subject:
Betanodavirus; Bohle iridovirus; Herpesviridae; Infectious pancreatic necrosis virus; Infectious spleen and kidney necrosis virus; Iridovirus; Lymphocystivirus; Tilapia lake virus; disease outbreaks; disease surveillance; mortality; pathogens; viruses
Abstract:
... There is an increasing number of emerging infectious agents, although not zoonotic, that affect farmed tilapines worldwide. Most recently, the emergence of a new virus, tilapia lake virus (TiLV), has diverted global attention to it while little or no research is being done on the other viruses of possible equal importance. These other viruses have reportedly been associated with relatively high mo ...
DOI:
10.1016/j.aquaculture.2019.01.036

85. The atypical chemokine receptor 4 in red sea bream (Pagrus major): Molecular characterization and gene expression analysis

Author:
Kwang-Min Choi; Seong Don Hwang; Min Soo Joo; Jee Youn Hwang; Mun-Gyeong Kwon; Ji-Min Jeong; Jung Soo Seo; Bo-Yeong Jee; Chan-Il Park
Source:
Fish & shellfish immunology 2019 v.93 pp. 50-54
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Pagrus major; Streptococcus iniae; amino acid sequences; chemokine receptors; chemokines; complementary DNA; cysteine; fish; gene expression; gene expression regulation; high-throughput nucleotide sequencing; immune response; immune system; kidneys; messenger RNA; nucleotide sequences; phylogeny
Abstract:
... Atypical chemokine receptor 4 (ACKR4) is regulated by cytokines, binds chemokines and regulates the chemokine gradient. We verified the cDNA sequence by confirming ACKR4 from red sea bream (PmACKR4) by next generation sequencing (NGS) and analysed the molecular characteristics and gene expression profile. In the analysis using the predicted amino acid sequence of PmACKR4, a highly conserved G prot ...
DOI:
10.1016/j.fsi.2019.07.004

86. The impact of pooling samples on surveillance sensitivity for the megalocytivirus Infectious spleen and kidney necrosis virus

Author:
Sophia J. Johnson; Paul M. Hick; Andrew P. Robinson; Anneke E. Rimmer; Alison Tweedie; Joy A. Becker
Source:
Transboundary and emerging diseases 2019 v.66 no.6 pp. 2318-2328
ISSN:
1865-1674
Subject:
Infectious spleen and kidney necrosis virus; detection limit; diagnostic sensitivity; emerging diseases; fish industry; hosts; import policies; monitoring; ornamental fish; pathogens; plasmids; quantitative polymerase chain reaction; screening; tissues; viral load; Australia
Abstract:
... Movements of large volumes and species varieties make the ornamental fish industry a high‐risk pathway for the transfer of aquatic pathogens to new geographical regions and naïve hosts, potentially resulting in emergency disease events. Infectious spleen and kidney necrosis virus (genus Megalocytivirus) is considered exotic to Australia despite documented incursions since 2003. There are current i ...
DOI:
10.1111/tbed.13288

87. Co-infection of infectious spleen and kidney necrosis virus and Francisella sp. in farmed pearl gentian grouper (♀Epinephelus fuscoguttatus ×♂E. lanceolatus) in China - A case report

Author:
Yucong Huang; Shuanghu Cai; Jichang Jian; Guangfeng Liu; Liwen Xu
Source:
Aquaculture 2020 v.526 pp. 735409
ISSN:
0044-8486
Subject:
Francisella; Infectious spleen and kidney necrosis virus; Iridovirus; Maccullochella peelii peelii; anorexia; case studies; females; fish farms; gills; grouper; histopathology; macrophages; males; mixed infection; mortality; phylogeny; polymerase chain reaction; sequence analysis; signs and symptoms (animals and humans); splenomegaly; swimming; viruses; China
Abstract:
... From October–December 2018, a mass mortality among cultured pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂E. lanceolatus) occurred on a private grouper farm in Zhanjiang City, Guangdong Province, China. Approximately 120 dead fish from each pond were recorded daily, and the cumulative mortality was up to 52% within 56 days. Diseased fish showed clinical signs such as lethargy, anorexia, slo ...
DOI:
10.1016/j.aquaculture.2020.735409

88. Current use and development of fish vaccines in China

Author:
Qingchao Wang; Wei Ji; Zhen Xu
Source:
Fish & shellfish immunology 2020 v.96 pp. 223-234
ISSN:
1050-4648
Subject:
Aeromonas hydrophila; Ctenopharyngodon idella; Edwardsiella tarda; Infectious spleen and kidney necrosis virus; Paralichthys olivaceus; Vibrio alginolyticus; antibodies; aquaculture industry; ethics; farmed fish; field experimentation; fish production; food safety; immune response; inactivated vaccines; live vaccines; markets; shellfish; sustainable development; turbot; vaccination; veterinary medicine; China
Abstract:
... In the past decades, the aquaculture industry made great progress in China, which contributes more than 70% yield of the world's farmed fish. Along with the rapid growth of fish production, increased emergence and outbreak of numbers of diseases pose harm to the aquaculture industry and food safety. From the efficient, safe, environmental and ethical aspects, vaccines is definitely the most approp ...
DOI:
10.1016/j.fsi.2019.12.010

89. Development and application of a sensitive droplet digital PCR (ddPCR) for the detection of infectious spleen and kidney necrosis virus

Author:
Qiang Lin; Xiaozhe Fu; Lihui Liu; Hongru Liang; Yinjie Niu; Yuanya Wen; Zhibin Huang; Ningqiu Li
Source:
Aquaculture 2020 v.529 pp. 735697
ISSN:
0044-8486
Subject:
DNA; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; detection limit; droplets; fish; fish industry; kidneys; monitoring; necrosis; quantitative polymerase chain reaction; spleen; viral load
Abstract:
... Infectious spleen and kidney necrosis, caused by the infectious spleen and kidney necrosis virus (ISKNV), has damaged the economy of the world fish industry. Droplet digital PCR (ddPCR) is a novel, sensitive, accurate and absolute quantitation method that does not require a standard curve. In this study, we established a sensitive ddPCR method to rapidly detect and quantify ISKNV DNA. The establis ...
DOI:
10.1016/j.aquaculture.2020.735697

90. Development of a high-dose vaccine formulation for prevention of megalocytivirus infection in rock bream (Oplegnathus fasciatus)

Author:
Woo Ju Kwon; Jae Chan Choi; Suhee Hong; Young Chul Kim; Min Gyeong Jeong; Joon Gyu Min; Joon Bum Jeong; Kwang Il Kim; Hyun Do Jeong
Source:
Vaccine 2020 v.38 no.51 pp. 8107-8115
ISSN:
0264-410X
Subject:
Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Pagrus major; adjuvants; aluminum hydroxide; antigens; bream; cell lines; dose response; mortality; squalene; water temperature
Abstract:
... A formalin-inactivated red sea bream iridovirus (RSIV) vaccine was prepared using the culture supernatant of a persistently infected Pagrus major fin cell line (PI-PMF) with IVS-1 strain (RSIV subtype II Meglaocytivirus). Rock bream (Oplegnathus fasciatus) were injected with a high-dose, ultracentrifuged megalocytivirus vaccine (Ultra HSCMV, 7.0 × 10¹⁰ copies/mL), a high-dose supernatant of cultur ...
DOI:
10.1016/j.vaccine.2020.11.001

91. EPA and DHA can modulate cell death via inhibition of the Fas/tBid-mediated signaling pathway with ISKNV infection in grouper fin cell line (GF-1) cells

Author:
Kuang-Wen Chen; Hsuan-Wen Chiu; Yu-Wei Chiu; Jen-Leih Wu; Jiann-Ruey Hong
Source:
Fish & shellfish immunology 2020 v.97 pp. 608-616
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; apoptosis; caspase-3; caspase-8; cell lines; cell membranes; cell viability; death; docosahexaenoic acid; eicosapentaenoic acid; fish diseases; gene expression regulation; genes; grouper; nucleotidyltransferases; signal transduction; viral load
Abstract:
... Polyunsaturated fatty acids (PUFAs) play important roles in organisms, including the structure and liquidity of cell membranes, anti-oxidation and anti-inflammation. Very little has been done in terms of the effect of PUFAs on cell death, especially on DNA virus. In this study, we demonstrated that the infectious spleen and kidney necrosis virus (ISKNV) can induce host cell death via the apoptotic ...
DOI:
10.1016/j.fsi.2019.10.029

92. Genomic and functional characterization of the lect2 gene from Siniperca chuatsi

Author:
Yawei Shen; Ming Cao; Shoujie Tang; Yan Zhao; Jinliang Zhao; Xiaowu Chen; Yanhui Bi
Source:
Fish & shellfish immunology 2020 v.107 pp. 146-155
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; artificial diets; brain; carnivores; chemoattractants; digestive physiology; domestication; fish; gene expression; gene expression regulation; genes; genomic islands; genomics; immunology; industrialization; intestines; kidneys; leukocytes; lipopolysaccharides; liver; methylation; muscles; polyinosinic-polycytidylic acid; promoter regions; shellfish; stomach; China
Abstract:
... Mandarin fish (Siniperca chuatsi) is an important economic fish in China. Viral and bacterial diseases seriously affect the artificial culture of S. chuatsi. As a carnivorous fish, artificial feed domestication is also an important means to improve the scale of S. chuatsi culture. Therefore, the study of immunology and digestive physiology is very important to the industrial development of S. chua ...
DOI:
10.1016/j.fsi.2020.09.034

93. Identification of giant gourami iridovirus (GGIV): a new infectious spleen and kidney necrosis virus (ISKNV) from natural outbreak in cultured Osphronemus goramy

Author:
Sukenda Sukenda; Lila Gardenia; M. Zairin Jr; Angela Lusiastuti; Alimuddin Alimudin
Source:
Aquaculture international 2020 v.28 no.3 pp. 1069-1082
ISSN:
0967-6120
Subject:
DNA; Infectious spleen and kidney necrosis virus; Iridovirus; Osphronemus; anorexia; coat proteins; financial economics; fish; fish diseases; food animals; genes; hemorrhage; histopathology; industry; intraperitoneal injection; kidneys; liver; mortality; pathogens; phylogeny; quantitative polymerase chain reaction; signs and symptoms (animals and humans); spleen; Indonesia
Abstract:
... Megalocytivirus of family Iridoviridae has been identified as a pathogen that caused the fatal systemic infection to lead to massive death of numerous fish species in both ornamental and food fish industries. It caused significant economic losses due to severe mortality of infected fish. Since 2011 in Indonesia, megalocytivirus in giant gourami (Osphronemus goramy) had been found as the main patho ...
DOI:
10.1007/s10499-020-00513-4

94. Identification of infectious spleen and kidney necrosis virus (ISKNV)-encoded microRNAs

Author:
Jian-hui He; Qiong Xia; Shaoping Weng; Jianguo He; Xiaopeng Xu
Source:
Virus genes 2020 v.56 no.6 pp. 724-733
ISSN:
0920-8569
Subject:
Infectious spleen and kidney necrosis virus; aquaculture; coat proteins; fish; gene expression; microRNA; prediction; quantitative polymerase chain reaction; viruses
Abstract:
... MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptionally regulate gene expression by complementary binding to target mRNAs. Virus-encoded miRNAs play important roles in virus life cycle and virus-host interactions. Viruses from the Megalocytivirus genus, family Iridoviridae, infect a wide range of fishes, bringing great challenges to aquaculture. Infectious spleen and kidney necrosi ...
DOI:
10.1007/s11262-020-01798-6

95. Identification of intron in ORF003 gene and its application for inactivation test of ISKNV

Author:
Xiaozhe Fu; Lixi Zhang; Lihui Liu; Qiang Lin; Hongru Liang; Yinjie Niu; Zhibin Huang; Ningqiu Li
Source:
Microbial pathogenesis 2020 v.138 pp. 103822
ISSN:
0882-4010
Subject:
DNA; DNA viruses; annealing; cost effectiveness; detection; fish; inactivated vaccines; introns; messenger RNA; oligodeoxyribonucleotides; reverse transcriptase polymerase chain reaction; sequence analysis; temperature; transcription (genetics); transcriptomics; viruses
Abstract:
... The virus inactivation test is a critical skill in inactivated vaccine production. Active viruses produced viral mRNA in susceptible cells or the host can be used to infer whether a DNA virus is replicating by RT-PCR. But it is generally difficult to avoid genomic DNA contamination in the samples. However, the use of primers spanning an intron is an effective alternative for virus inactivation tes ...
DOI:
10.1016/j.micpath.2019.103822

96. Impact of Aeromonas hydrophila and infectious spleen and kidney necrosis virus infections on susceptibility and host immune response in Chinese perch (Siniperca chuatsi)

Author:
Xiaodan Liu; Wei Sun; Yanbing Zhang; Yifan Zhou; Jingwen Xu; Xiaojian Gao; Shuangming Zhang; Xiaojun Zhang
Source:
Fish & shellfish immunology 2020 v.105 pp. 117-125
ISSN:
1050-4648
Subject:
Aeromonas hydrophila; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; fish; hepcidin; histopathology; immune response; immune system; inflammation; mixed infection; mortality; secondary infection; shellfish; viral load
Abstract:
... Co-infections with pathogenic microorganisms are common in aquaculture, resulting in more serious economic losses than single-pathogen infection. Infection of Aeromonas hydrophila (A. hydrophila) often occurs together with infectious spleen and kidney necrosis virus (ISKNV) in Chinese perch (Siniperca chuatsi) culture ponds. In this study, A. hydrophila and ISKNV were inoculated into Chinese perch ...
DOI:
10.1016/j.fsi.2020.07.012

97. In vivo efficacy of ganciclovir by using single-walled carbon nanotubes as a drug carrier against infectious spleen and kidney necrosis virus in mandarin fish

Author:
Zhao Zhao; Chen Zhang; Yi-Jun Jia; Gao-Xue Wang; Bin Zhu
Source:
Aquaculture 2020 v.526 pp. 735434
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; antiviral agents; carbon nanotubes; complement; drug carriers; drugs; enzyme activity; fish; fish diseases; gastrointestinal system; genes; mortality; plant diseases and disorders; plant viruses
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) cause a high mortality disease which leads to a significant economic loss on mandarin fish in China. At present, there is no effective drug against this fatal disease because of several impenetrable barriers (cell, skin and gastrointestinal tract). In this study, we selected ganciclovir for the treatment of ISKNV and choose single-walled carbon n ...
DOI:
10.1016/j.aquaculture.2020.735434

98. Myxovirus resistance (Mx) gene and its differential expression regulated by three type I and two type II IFNs in mandarin fish, Siniperca chuatsi

Author:
Li Li; Shan Nan Chen; Zubair Ahmed Laghari; Hui Jun Huo; Jing Hou; Lin Huang; Nan Li; P. Nie
Source:
Developmental and comparative immunology 2020 v.105 pp. 103604
ISSN:
0145-305X
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; fish; gene expression regulation; genes; interferon-gamma; protein content; response elements; tissues; virus replication
Abstract:
... Interferons (IFNs) can induce the expression of IFN-stimulated genes (ISGs), such as myxovirus resistance (Mx) protein, to inhibit virus replication. In this study, the expression of Mx gene in mandarin fish, and the IFN-sensitive response elements (ISREs) and gamma-interferon activated sites (GASs) in the promoter of Mx gene were analyzed in relation to the stimulation of three distinct type I IF ...
DOI:
10.1016/j.dci.2019.103604

99. Novel peptide nucleic acid-based real-time PCR assay for detection and genotyping of Megalocytivirus

Author:
Eun Sun Lee; Miyoung Cho; Eun Young Min; Sung Hee Jung; Kwang Il Kim
Source:
Aquaculture 2020 v.518 pp. 734818
ISSN:
0044-8486
Subject:
DNA; Infectious spleen and kidney necrosis virus; Pagrus major; Turbot reddish body iridovirus; animal diseases; aquatic animals; coat proteins; detection limit; diagnostic techniques; etiological agents; fluorescence; genes; genotype; genotyping; hybridization; melting; melting point; nucleic acid hybridization; peptides; phylogeny; quantitative polymerase chain reaction; sequence analysis
Abstract:
... Megalocytivirus, the causative agent for Red Sea Bream Iridoviral Disease (RSIVD), is classified into three genotypes based on its phylogeny: Red Sea Bream Iridovirus (RSIV), Infectious Spleen and Kidney Necrosis Virus (ISKNV), and Turbot Reddish Body Iridovirus (TRBIV). To overcome drawbacks such as limitation of genotypic variant type discrimination and unexpected PCR reaction from an SYBR green ...
DOI:
10.1016/j.aquaculture.2019.734818

100. Optimization of the efficacy of a SWCNTs-based subunit vaccine against infectious spleen and kidney necrosis virus in mandarin fish

Author:
Zhao Zhao; Yan Xiong; Chen Zhang; Yi-Jun Jia; De-Kui Qiu; Gao-Xue Wang; Bin Zhu
Source:
Fish & shellfish immunology 2020 v.106 pp. 190-196
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; antibody formation; blood serum; enzymes; fish; fish culture; mortality; shellfish; subunit vaccines; vaccination; China
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) cause a high mortality disease which brings substantial economic losses to the mandarin fish culture industry in China. This study was aimed at optimizing the efficacy of a SWCNTs-based immersion subunit vaccine (SWCNTs-M-MCP) which as a promising vaccine against ISKNV. Mandarin fish were vaccinated by immersion, then we designed an orthogonal ex ...
DOI:
10.1016/j.fsi.2020.07.062