U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject term Infectious spleen and kidney necrosis virus Remove constraint Subject term: Infectious spleen and kidney necrosis virus
Start Over

Search

150  Search Results

« Previous | 1 - 100 of 150 |
Number of results to display per page

Search Results

1. Characterization and function of mandarin fish c-Myc during viral infection process

Author:
Caimei Ye; Ningqiu Li; Yinjie Niu; Qiang Lin; Xia Luo; Hongru Liang; Lihui Liu; Xiaozhe Fu
Source:
Fish & shellfish immunology 2022 v.120 pp. 686-694
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Rhabdoviridae; Siniperca chuatsi; amino acid sequences; brain; fish; glutamine; immunology; kidneys; metabolism; open reading frames; protein content; shellfish; spleen; transcription factors; virus replication
Abstract:
... c-Myc is a transcription factor and master regulator of cellular metabolism, and plays a critical role in virus replication by regulating glutamine metabolism. In this study, the open-reading frame (ORF) of c-Myc, designated as Sc-c-Myc, was cloned and sequenced. Multiple alignment of the amino acid sequence showed that the conserved domain of Sc-c-Myc, including the helix–loop–helix-zipper (bHLHz ...
DOI:
10.1016/j.fsi.2021.12.045

2. Deleting ORF71L of infectious spleen and kidney necrosis virus (ISKNV) resulted in virulence attenuation in Mandarin fish

Author:
Hetong Zhang; Hemei Qi; Shaoping Weng; Jianguo He; Chuanfu Dong
Source:
Fish & shellfish immunology 2022 v.123 pp. 335-347
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; aquaculture industry; fish fry; genes; homologous recombination; immunology; mortality; mutants; peptides; protein synthesis; shellfish; structural proteins; transcriptomics; viral genome; virion; virulence; viruses
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV), the type species of the genus Megalocytivirus, infects a variety of teleost fish species and causes substantial losses in the aquaculture industry worldwide. ISKNV ORF71L is 1611 bp in length, encodes a 537-amino-acid peptide and was previously identified as a viral structural protein in the ISKNV virion. In this study, the ORF71L deletion mutan ...
DOI:
10.1016/j.fsi.2022.02.041

3. Development and application of a sensitive droplet digital PCR for the detection of red mark syndrome infection in rainbow trout (Oncorhynchus mykiss)

Author:
M. Orioles; M. Bulfoni; E. Saccà; D. Cesselli; J.G. Schmidt; M. Galeotti
Source:
Aquaculture 2022 v.551 pp. 737910
ISSN:
0044-8486
Subject:
DNA; Infectious spleen and kidney necrosis virus; Oncorhynchus mykiss; aquaculture; detection limit; droplets; etiology; markets; monitoring; quantitative polymerase chain reaction; spleen
Abstract:
... Red Mark Syndrome (RMS) is a skin disease of uncertain etiology affecting farmed rainbow trout (Oncorhynchus mykiss). It consists of single or multiple skin lesions usually localized on the trunk of fish approaching market size. Studies showed as rickettsia-like organism (RLO), currently referred as Midichloria-like organism (MLO), is supposed to be involved in RMS. Midichloria-like has been ident ...
DOI:
10.1016/j.aquaculture.2022.737910

4. Development of two cell lines from yellowfin seabream Acanthopagrus latus fin and brain suitable for propagating SDDV but not for ISKNV, MRV and GIV

Author:
Yuting Fu; Yong Li; Xiangrong Liu; Weixuan Fu; Shaoping Weng; Fangzhao Yu; Jianguo He; Chuanfu Dong
Source:
Aquaculture 2022 v.560 pp. 738478
ISSN:
0044-8486
Subject:
Acanthopagrus; Infectious spleen and kidney necrosis virus; Iridovirus; Ranavirus; Western blotting; aquaculture; ascites; brain; cell culture; cell lines; chromosomes; cytopathogenicity; etiological agents; fetal bovine serum; fluorescent antibody technique; grouper; karyotyping; quantitative polymerase chain reaction; transmission electron microscopy; vaccines; viruses; China
Abstract:
... Scale drop disease virus (SDDV), a very distinct member of genus Megalocytivirus in family Iridoviridae, was recently confirmed as the most alarming causative agent associated with yellowfin seabream Acanthopagrus latus ascites diseases in South China. To better understand how host-pathogen interacting and developing cell-culture based vaccine, establishment of highly susceptible cell line generat ...
DOI:
10.1016/j.aquaculture.2022.738478

5. Establishment and characterization of a permanent heart cell line from largemouth bass Micropterus salmoides and its application to fish virology and immunology

Author:
Weiwei Zeng; Hanxu Dong; Xiaoyu Chen; Sven M. Bergmann; Ying Yang; Xinxian Wei; Guixiang Tong; Hua Li; Hui Yu; Yanfeng Chen
Source:
Aquaculture 2022 v.547 pp. 737427
ISSN:
0044-8486
Subject:
Betanodavirus; Carp sprivivirus; Ctenopharyngodon idella; Infectious spleen and kidney necrosis virus; Micropterus salmoides; Mycoplasma; Ranavirus; Reoviridae; Tilapia lake virus; aquaculture; cell lines; cytochrome-c oxidase; diploidy; electron microscopy; farmed fish; fetal bovine serum; gene expression; genotype; heart; industry; interferons; karyotyping; reporter genes; virology; China
Abstract:
... Largemouth bass (Micropterus salmoides) is among the most important cultured fish species in China and various viral diseases have greatly affected the largemouth bass industry. We established a permanent cell line from the heart of M. salmoides (MSH) that has been subcultured over 70 times and showed optimal growth at 27 °C using Medium 199 supplemented with 10% fetal bovine serum. MSH cells cons ...
DOI:
10.1016/j.aquaculture.2021.737427

6. Establishment and characterization of a spinal cord tissue cell line from mandarin fish, Siniperca chuatsi and its susceptibility to several viruses

Author:
Jinpeng Gong; Xiaoyi Pan; Lingyun Lin; Yue Zhu; Jiayun Yao; Congxu Wang; Wenlin Yin; Lei Huang; Yihan Liu; Fan Chen; Jinyu Shen
Source:
Journal of fish diseases 2022 v.45 no.10 pp. 1419-1427
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Ranavirus; Rhabdoviridae; Siniperca chuatsi; cell lines; cytochrome-c oxidase; diploidy; fetal bovine serum; fish; karyotyping; spinal cord; transmission electron microscopy
Abstract:
... In this study, we established and characterized a continuous cell line from the spinal cord tissue of mandarin fish, Siniperca chuatsi and assessed its susceptibility to infectious spleen and kidney necrosis virus (ISKNV), Siniperca chuatsi ranavirus (SCRaV) and Siniperca chuatsi rhabdovirus (SCRV). The cell line, named SCC, has been successively cultured up to 40 passages. The optimal growing con ...
DOI:
10.1111/jfd.13671

7. First report of infectious spleen and kidney necrosis virus in Nile tilapia in Brazil

Author:
Henrique César Pereira Figueiredo; Guilherme Campos Tavares; Fernanda Alves Dorella; Júlio César Câmara Rosa; Sóstenes Apolo Correia Marcelino; Felipe Pierezan; Felipe Luiz Pereira
Source:
Transboundary and emerging diseases 2022 v.69 no.5 pp. 3008-3015
ISSN:
1865-1674
Subject:
Aeromonas hydrophila; Edwardsiella tarda; Infectious spleen and kidney necrosis virus; Oreochromis niloticus; Streptococcus agalactiae; aquaculture; fish; fish farms; histopathology; mortality; pathogens; phylogeny; spleen; transmission electron microscopy; viruses; Brazil; Ghana; Thailand
Abstract:
... In June 2020, an atypical fatal outbreak in a Brazilian Nile tilapia farm was investigated. Twenty‐three animals were collected and different tissues were used for bacterial isolation, histopathological and electron microscopic examination and viral detection using molecular methods. A large number of megalocytes were observed in the histopathological analysis of several tissues. Icosahedral virio ...
DOI:
10.1111/tbed.14217

8. Generation of monoclonal antibodies against heterologously expressed major capsid protein of infectious spleen and kidney necrosis virus (ISKNV)

Author:
Akapon Vaniksampanna; Orapan Manajit; Saengchan Senapin; Suphattra Kamsamarn; Pradit Wangman; Siwapon Longyant; Parin Chaivisuthangkura
Source:
Aquaculture 2022 pp. 738895
ISSN:
0044-8486
Subject:
Betanodavirus; Escherichia coli; Infectious spleen and kidney necrosis virus; Lates calcarifer; Tilapia lake virus; aquaculture; cell culture; coat proteins; cross reaction; detection limit; fish; genes; genotype; glutathione transferase; immunization; immunohistochemistry; intestines; kidneys; liver; monoclonal antibodies; phylogeny; spleen; stomach; viruses
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) is one of the detrimental viruses causing economic losses in several fish species including Asian seabass (Lates calcarifer). In this study, monoclonal antibodies specific to major capsid protein (MCP) of ISKNV were generated. The MCP encoding gene of ISKNV genotype II was cloned into pGEX-6P-1 and transformed into E. coli. After induction, a glu ...
DOI:
10.1016/j.aquaculture.2022.738895

9. Major histocompatibility IIβ diversity and peptide-binding groove properties associated with red sea bream iridovirus resistance

Author:
Eitaro Sawayama; Shiho Tanizawa; Kei Nakayama; Risa Ito; Yuri Akase; Shin-Ichi Kitamura
Source:
Aquaculture 2022 v.552 pp. 738038
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; Pagrus major; alleles; amino acid sequences; amino acids; breeding stock; disease resistance; farmed fish; haplotypes; hydrophilicity; hydrophobicity; livestock; loci; major histocompatibility complex
Abstract:
... Major histocompatibility complex (MHC) II genes are key factors in presenting antigens derived from extracellular pathogens to CD4⁺ T cells. These genes are known to be associated with disease resistance in livestock, including cultured fish species. Genome-wide linkage analysis of RSIV-resistant red sea bream has revealed that MHC IIβ is linked to the RSIV-resistant trait and was suggested as a p ...
DOI:
10.1016/j.aquaculture.2022.738038

10. MicroRNA expression and analysis of immune-related putative target genes in ISKNV-infected spleen of mandarin fish (Siniperca chuatsi)

Author:
Yu Zhou; Ying-Ying Wang; Huang-Cui Fu; He-Zhong Huang
Source:
Aquaculture 2022 v.547 pp. 737450
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; aquaculture; autophagy; cell cycle; endocytosis; endoplasmic reticulum; fish; immune response; innate immunity; lysosomes; microRNA; pathogens; protein transport; salmonellosis; spleen; vascular endothelial growth factors
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV), a highly infectious and lethal pathogen of mandarin fish (Siniperca chuatsi), has resulted in major aquaculture losses over the past few decades. A lack of understanding of the regulatory mechanism of viral infection has impeded the development of effective measures to control ISKNV infection. MicroRNAs (miRNAs) are a class of non-coding RNAs th ...
DOI:
10.1016/j.aquaculture.2021.737450

11. Molecular and functional characterization of zinc finger aspartate-histidine-histidine-cysteine (DHHC)-type containing 1, ZDHHC1 in Chinese perch Siniperca chuatsi

Author:
Shan Nan Chen; Shan Zhang; Li Li; Zubair Ahmed Laghari; Pin Nie
Source:
Fish & shellfish immunology 2022 v.130 pp. 215-222
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; amino acids; fish; genes; humans; immunology; shellfish; zinc
Abstract:
... In the present study, the zinc finger aspartate-histidine-histidine-cysteine (DHHC)-type containing 1 (ZDHHC1) gene was identified in a commercial fish, the Chinese perch Siniperca chuatsi. The ZDHHC1 has five putative transmembrane motifs and conserved DHHC domain, showing high amino-acid identity with other teleost fish, and vertebrate ZDHHC1 loci are conserved from fish to human. In vivo express ...
DOI:
10.1016/j.fsi.2022.09.023

12. Molecular characterization and transcriptional conservation of N-myc-interactor, Nmi, by type I and type II IFNs in mandarin fish Siniperca chuatsi

Author:
Li Li; Shan Nan Chen; Nan Li; P. Nie
Source:
Developmental and comparative immunology 2022 v.130 pp. 104354
ISSN:
0145-305X
Subject:
DNA; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; amino acid sequences; apoptosis; blood; carcinogenesis; exons; fish; gene expression; inflammation; innate immunity; introns; luciferase; mammals; protein content; transcription (genetics)
Abstract:
... N-myc-interactor (Nmi) belongs to interferon (IFN) stimulated genes (ISGs) and is involved in the regulation of physiological processes including viral infection, inflammatory response, apoptosis and tumorigenesis in mammals. However, the function of Nmi in teleost fish remains to be explored. In this study, an Nmi homologue was characterized from mandarin fish Siniperca chuatsi. The mandarin fish ...
DOI:
10.1016/j.dci.2022.104354

13. Neomycin inhibits Megalocytivirus infection in fish by antagonizing the increase of intracellular reduced glutathione

Author:
Hengwei Deng; Yeyu Li; Jinling Li; Wenjie Shen; Qiankang Chen; Shaoping Weng; Jianguo He; Xiaopeng Xu
Source:
Fish & shellfish immunology 2022 v.127 pp. 148-154
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; aquaculture; coat proteins; freshwater; glutathione; immunology; neomycin; reactive oxygen species; shellfish; therapeutics
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) is the type species of the Megalocytivirus genus that infects a number of marine and freshwater fishes, causing huge economic losses in aquaculture. The ISKNV infection leads to increase of reducing power in cells. As the antibiotic neomycin can promote the production of reactive oxygen species (ROS) in animal cells, in the current study, the pot ...
DOI:
10.1016/j.fsi.2022.06.016

14. PI3K/AKT/p53 pathway inhibits infectious spleen and kidney necrosis virus infection by regulating autophagy and immune responses

Author:
Xiaoting Zhang; Yue Ming; Xiaozhe Fu; Yinjie Niu; Qiang Lin; Hongru Liang; Xia Luo; Lihui Liu; Ningqiu Li
Source:
Fish & shellfish immunology 2022 v.120 pp. 648-657
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; antiviral properties; autophagy; brain; fish; genes; industry; shellfish
Abstract:
... The PI3K/AKT/p53 signaling pathway is activated by various types of cellular stimuli or pathogenic infection, and then regulates fundamental cellular functions to combat these stimulations. Here, we studied the meaningful roles of PI3K/AKT/p53 in regulating cellular machine such as autophagy, immune responses, as well as antiviral activity in Chinese perch brain (CPB) cells infected by infectious ...
DOI:
10.1016/j.fsi.2021.12.046

15. Protective immunity against infectious spleen and kidney necrosis virus induced by mannose modified subunit vaccine with carbon nanotubes in mandarin fish

Author:
Zhao Zhao; Yang Li; Guo Chen; Chen Zhang; Gao‐Xue Wang; Bin Zhu
Source:
Aquaculture research 2022 v.53 no.6 pp. 2175-2184
ISSN:
1355-557X
Subject:
Infectious spleen and kidney necrosis virus; antibodies; antigens; aquaculture; carbon nanotubes; coat proteins; enzymes; fish; host range; immunization; immunogenicity; mannose; mortality; research; subunit vaccines; viruses; China
Abstract:
... Due to its wide host range and frequently causing significant mortality, infectious spleen and kidney necrosis virus (ISKNV) has caused huge economic losses on mandarin fish in China. There are no commercial drugs to cure ISKNV, and vaccines are regarded as one of the most effective measures to control virus infection. Because of the weak immunogenicity of antigen protein, subunit vaccines may req ...
DOI:
10.1111/are.15736

16. Quick hassle-free detection of cyprinid herpesvirus 2 (CyHV-2) in goldfish using recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay

Author:
Prasannan Geetha Preena; Thaliyil Veetil Arun Kumar; Tina Kollannoor Johny; Arathi Dharmaratnam; Thangaraj Raja Swaminathan
Source:
Aquaculture international 2022 v.30 no.3 pp. 1211-1220
ISSN:
0967-6120
Subject:
Betanodavirus; Carassius auratus; Carassius gibelio; Carp sprivivirus; Cyprinid herpesvirus 2; Cyprinid herpesvirus 3; Infectious spleen and kidney necrosis virus; aquaculture; coat proteins; cross reaction; detection limit; fish health; genes; goldfish; mortality; necrosis; ornamental fish; point-of-care testing; recombinase polymerase amplification; recombinases
Abstract:
... Cyprinid herpesvirus 2 (CyHV-2) is the etiological agent of herpesviral hematopoietic necrosis disease (HVHND), which causes severe mortality in ornamental goldfish (Carassius auratus), crucian carp (Carassius auratus), and gibel/prussian carp (Carassius gibelio). Quick and hassle-free point-of-care detection of CyHV-2 is vital for the maintenance of ornamental fish health. In this manuscript, we ...
DOI:
10.1007/s10499-021-00806-2

17. Red Sea Bream Iridovirus (RSIV) Kinetics in Rock Bream (Oplegnathus fasciatus) at Various Fish-Rearing Seawater Temperatures

Author:
Kyung-Ho Kim; Kwang-Min Choi; Min-Soo Joo; Gyoungsik Kang; Won-Sik Woo; Min-Young Sohn; Ha-Jeong Son; Mun-Gyeong Kwon; Jae-Ok Kim; Do-Hyung Kim; Chan-Il Park
Source:
Animals 2022 v.12 no.15 pp. -
ISSN:
2076-2615
Subject:
Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Pagrus major; aquaculture industry; bream; genome; mortality; seawater; virus replication; viruses; water temperature
Abstract:
... Red sea bream iridoviral disease (RSIVD) causes serious economic losses in the aquaculture industry. In this paper, we evaluated RSIV kinetics in rock bream under various rearing water temperatures and different RSIV inoculation concentrations. High viral copy numbers (approximately 10³.⁷–10⁶.⁷ RSIV genome copies/L/g) were observed during the period of active fish mortality after RSIV infection at ...
DOI:
10.3390/ani12151978

18. Red sea bream interleukin (IL)-1β and IL-8 expression, subcellular localization, and antiviral activity against red sea bream iridovirus (RSIV)

Author:
Min-Soo Joo; Kwang-Min Choi; Gyoungsik Kang; Won-Sik Woo; Kyung-Ho Kim; Min-Young Sohn; Ha-Jeong Son; Hyun-Ja Han; Hye-Sung Choi; Do-Hyung Kim; Chan-Il Park
Source:
Fish & shellfish immunology 2022 v.128 pp. 360-370
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Pagrus major; antiviral properties; chemotaxis; cytoplasm; fish; inflammation; interferon-gamma; interleukin-10; interleukin-1beta; interleukin-8; kidneys; ligands; liver; macrophages; monocytes; neutrophils; pathogens; shellfish; spleen
Abstract:
... Interleukin-1 beta (IL-1β) is transcribed by monocytes, macrophages, and dendritic cells in response to activation of toll-like receptors (TLRs) by pathogen-associated molecular patterns (PAMPs) or cytokine signalling and causes a rapid inflammatory response to infection. IL-8, also known as chemokine C-X-C motif ligand (CXCL)-8, is regulated by IL-1β and affects the chemotaxis of macrophages and ...
DOI:
10.1016/j.fsi.2022.07.040

19. Red sea bream iridovirus infection in cage farmed Asian sea bass (Lates calcarifer): Insights into the pathology, epizootiology, and genetic diversity

Author:
T.G. Sumithra; S.R. Krupesha Sharma; L. Neelima; N.R. Dhanutha; Aswathy Joshy; V.N. Anusree; S. Gayathri; R.K. Raghu; N.D. Praveen; Sujitha Thomas; K.M. Rajesh
Source:
Aquaculture 2022 v.548 pp. 737571
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; Lates calcarifer; Vibrio alginolyticus; Vibrio fluvialis; adenosinetriphosphatase; anemia; brain; cages; carrier state; disease outbreaks; genes; genetic variation; host range; immune response; kidneys; liver; mortality; pathogenesis; pathogens; quarantine; socioeconomics; spleen; splenomegaly; transmission electron microscopy; viruses; India
Abstract:
... Red sea bream iridovirus (RSIV) is an emerging viral pathogen having significant socio-economic and environmental impacts on marine and brackish aquaculture systems. The study documents certain interesting findings of an RSIV disease outbreak that caused 50% cumulative mortality among cage farmed Asian sea bass in India. The confirmatory diagnosis was made based on internationally approved protoco ...
DOI:
10.1016/j.aquaculture.2021.737571

20. Virulence difference between red sea bream iridovirus mixed subtype I/II and subtype II and the expression of viral and apoptosis-related genes in infected rock bream (Oplegnathus fasciatus)

Author:
Min A Jeong; Ye Jin Jeong; Kwang Il Kim
Source:
Fish & shellfish immunology 2022 v.127 pp. 195-202
ISSN:
1050-4648
Subject:
DNA; Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; apoptosis; bream; caspase-3; caspase-8; gene expression; immunology; intraperitoneal injection; mortality; mutation; odds ratio; relative risk; shellfish; spleen; virulence; virus transmission
Abstract:
... In this study, the virulence of the red sea bream iridovirus (RSIV) subtype II (17RbGs isolate) and a novel RSIV mixed subtype I/II (17SbTy isolate), which was genetically characterized in a previous study, were compared. The infectivity to rock bream (Oplegnathus fasciatus) determined by infectious dose (ID₅₀) revealed that 17RbGs isolate was significantly more infective than 17SbTy isolate using ...
DOI:
10.1016/j.fsi.2022.05.041

21. Whole‐genome analysis of red sea bream iridovirus spread in 2021 in Japan provided epidemiological and viral traits insight

Author:
Hikaru Ishihara; Shogo Harakawa; Hidemasa Kawakami; Keisuke Yoshii; Naoya Murase; Hidetoshi Yamada; Yutaka Fukuda; Reiko Nozaki; Satoshi Kawato; Keiichiro Koiwai; Ikuo Hirono; Hidehiro Kondo
Source:
Journal of fish diseases 2022 v.45 no.10 pp. 1593-1597
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Japan; Pagrus major; ancestry; aquaculture industry; fish; hosts; pathogenicity; pathogens; phylogeny
Abstract:
... Red sea bream iridovirus (RSIV) is the pathogen that causes red sea bream iridoviral disease. It causes a huge loss to the Japanese aquaculture industry. In 2021, outbreaks of red sea bream iridovirus occurred in South Japan. This study analysed nine whole‐genome sequences of RSIV isolated in Oita and Ehime Prefectures in 2021 using a short‐read next‐generation sequencer. Nine isolates had highly ...
DOI:
10.1111/jfd.13690

22. The dynamic immune responses of Mandarin fish (Siniperca chuatsi) to ISKNV in early infection based on full-length transcriptome analysis and weighted gene co-expression network analysis

Author:
Yu Zhou; Huang-Cui Fu; Ying-Ying Wang; He-Zhong Huang; Xiao-Zhe Fu; Ning-Qiu Li
Source:
Fish & shellfish immunology 2022 v.122 pp. 191-205
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; apoptosis; endocytosis; fish; gene expression regulation; genes; herpes simplex; immune response; interleukin-8; intestines; phagosomes; sequence analysis; shellfish; spleen; transcription factor NF-kappa B; transcriptome; transcriptomics; viruses
Abstract:
... Mandarin fish (Siniperca chuatsi) been seriously harmed by infectious spleen and kidney necrosis virus (ISKNV) in recent years, but the early immune response mechanism of infection is still unknown. Here, we performed RNA sequencing on the spleens of mandarin fish infected with ISKNV at 0, 12, 24, 48, and 72 h post-infection (hpi) using short-read Illumina RNA sequencing and long-read Pacific Bios ...
DOI:
10.1016/j.fsi.2022.02.017

23. A natural outbreak of infectious spleen and kidney necrosis virus threatens wild pearlspot, Etroplus suratensis in Peechi Dam in the Western Ghats biodiversity hotspot, India

Author:
Thangaraj Raja Swaminathan; Tina Kollannoor Johny; Sundar Raj Nithianantham; Arun Sudhagar; Pravata Kumar Pradhan; Krupesha Sharma Sulumane Ramachandra; Reshma R. Nair; Neeraj Sood
Source:
Transboundary and emerging diseases 2022 v.69 no.5 pp. e1595
ISSN:
1865-1674
Subject:
Etroplus suratensis; Infectious spleen and kidney necrosis virus; biodiversity; body length; body weight; cell lines; coat proteins; fish communities; histopathology; host range; irrigation; lakes; liver; mortality; nucleotide sequences; pathogens; phylogeny; shrinkage; spleen; transmission electron microscopy; viral load; viruses; water supply; wild fish; India
Abstract:
... A large‐scale mortality of pearlspot, Etroplus suratensis was reported from Peechi Dam, an artificial tropical lake made for irrigation and drinking water supply in Kerala, India during 2018. This dam is located in the premises of Western Ghats, recognized as one of the biodiversity hotspots of the world. The objective of this study was to identify the aetiological agent of this large‐scale mortal ...
DOI:
10.1111/tbed.14494

24. Characterization of mandarin fish (Siniperca chuatsi) IL-6 and IL-6 signal transducer and the association between their SNPs and resistance to ISKNV disease

Author:
Rui-Ming Jin; He-Zhong Huang; Yu Zhou; Ying-Ying Wang; Huang-Cui Fu; Ze Li; Xiao-Zhe Fu; Ning-Qiu Li
Source:
Fish & shellfish immunology 2021 v.113 pp. 139-147
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; alleles; disease resistance; fish; hepatocytes; inflammation; interleukin-6; kidneys; loci; metabolism; quantitative polymerase chain reaction; shellfish; signal transduction; spleen; statistical analysis
Abstract:
... In fish, interleukin-6 (IL-6) is a very important immune-regulatory cytokine that plays a polyfunctional role in inflammation, metabolism, regeneration, and neural processes. IL-6 signal transducer (IL-6ST) is a specific receptor for IL-6 and expressed mainly in immune cells and hepatocytes. In this study, the complete cDNA and genomic DNA sequences of mandarin fish (Siniperca chuatsi) IL-6 and IL ...
DOI:
10.1016/j.fsi.2021.04.003

25. Complete Genome Sequences and Pathogenicity Analysis of Two Red Sea Bream Iridoviruses Isolated from Cultured Fish in Korea

Author:
Min-A Jeong; Ye-Jin Jeong; Kwang-Il Kim
Source:
Fishes 2021 v.6 no.4 pp. -
ISSN:
2410-3888
Subject:
DNA-binding proteins; Infectious spleen and kidney necrosis virus; Lateolabrax japonicus; Oplegnathus fasciatus; Pagrus major; adenosinetriphosphatase; bream; coat proteins; etiological agents; farmed fish; genomics; membrane proteins; pathogenicity; sequence homology; Korean Peninsula
Abstract:
... In Korea, red sea bream iridovirus (RSIV), especially subtype II, has been the main causative agent of red sea bream iridoviral disease since the 1990s. Herein, we report two Korean RSIV isolates with different subtypes based on the major capsid protein and adenosine triphosphatase genes: 17SbTy (RSIV mixed subtype I/II) from Japanese seabass (Lateolabrax japonicus) and 17RbGs (RSIV subtype II) fr ...
DOI:
10.3390/fishes6040082

26. Complete genome analysis of a red seabream iridovirus (RSIV) isolated from Asian seabass (Lates calcarifer) in India

Author:
T.G. Puneeth; Pallavi Baliga; S.K. Girisha; Malathi Shekar; M.S. Nithin; T. Suresh; B.T. Naveen Kumar
Source:
Virus research 2021 v.291 pp. 198199
ISSN:
0168-1702
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; Lates calcarifer; Pagrus major; adenosinetriphosphatase; coat proteins; etiological agents; farmed fish; genes; mortality; phylogeny; research; sequence analysis; viruses; India
Abstract:
... Red sea bream iridovirus (RSIV) is the causative agent of the iridoviral disease with high mortality rates in cultured fish. Our laboratory reported the first case of RSIV infection in India which resulted in mass mortalities of Asian seabass, Lates calcarifer. The RSIV-LC strain isolated from infected fish was subjected to complete genome sequencing and analysis. The complete genome of RSIV-LC wa ...
DOI:
10.1016/j.virusres.2020.198199

27. Complete genome sequences of infectious spleen and kidney necrosis virus isolated from farmed albino rainbow sharks Epalzeorhynchos frenatum in the United States

Author:
Samantha A. Koda; Kuttichantran Subramaniam; Deborah B. Pouder; Roy P. Yanong; Salvatore Frasca Jr; Vsevolod L. Popov; Thomas B. Waltzek
Source:
Virus genes 2021 v.57 no.5 pp. 448-452
ISSN:
0920-8569
Subject:
DNA; Infectious spleen and kidney necrosis virus; albino; genotype; histopathology; kidneys; spleen; transmission electron microscopy; viruses
Abstract:
... The genus Megalocytivirus includes viruses known to cause significant disease in aquacultured fish stocks. Herein, we report the complete genome sequences of two megalocytiviruses (MCVs) isolated from diseased albino rainbow sharks Epalzeorhynchos frenatum reared on farms in the United States in 2018 and 2019. Histopathological examination revealed typical megalocytivirus microscopic lesions (i.e. ...
DOI:
10.1007/s11262-021-01857-6

28. Development of cross‐priming amplification (CPA) combined with colorimetric and lateral flow dipstick visualization for scale drop disease virus (SDDV) detection

Author:
Terawut Prasitporn; Saengchan Senapin; Akapon Vaniksampanna; Siwaporn Longyant; Parin Chaivisuthangkura
Source:
Journal of fish diseases 2021 v.44 no.9 pp. 1411-1422
ISSN:
0140-7775
Subject:
DNA; Infectious spleen and kidney necrosis virus; Lates calcarifer; Redspotted grouper nervous necrosis virus; coat proteins; colorimetry; cross reaction; fish; genes; temperature; uracil-DNA glycosylase; viruses
Abstract:
... Scale drop disease virus (SDDV) is one of the most important pathogens that causes scale drop disease (SDD) in Asian sea bass (Lates calcarifer). The outbreaks of this disease are one of the factors causing substantial losses in Asian sea bass aquaculture. In this study, the uracil‐DNA glycosylase (UDG)‐supplemented cross‐priming amplification (UCPA) combined with a colorimetric detection method u ...
DOI:
10.1111/jfd.13448

29. Development of single nucleotide polymorphism (SNP) application for detection and genotyping of RSIV‐type megalocytiviruses

Author:
Channapha Sakseepipad; Reiko Nozaki; Keisuke Yoshii; Yutaka Fukuda; Kaori Mizuno; Hidemasa Kawakami; Ikuo Hirono; Hidehiro Kondo
Source:
Journal of fish diseases 2021 v.44 no.9 pp. 1337-1342
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Japan; genome; genotyping; haplotypes; mortality; phylogeny; single nucleotide polymorphism; viruses
Abstract:
... Red sea bream iridovirus (RSIV) belonging to the genus Megalocytivirus of the family Iridoviridae is the cause of serious mass mortality of cultured marine fishes. RSIV‐type megalocytiviruses show extremely high nucleotide sequence identities. Thus, epidemiological studies on this virus are limited. This study developed two primer sets amplifying the regions possessing single nucleotide polymorphi ...
DOI:
10.1111/jfd.13392

30. First detection of infectious spleen and kidney necrosis virus (ISKNV) associated with massive mortalities in farmed tilapia in Africa

Author:
José Gustavo Ramírez‐Paredes; Richard K. Paley; William Hunt; Stephen W. Feist; David M. Stone; Terence R. Field; David J. Haydon; Peter A. Ziddah; Mary Nkansa; James Guilder; Joshua Gray; Samuel Duodu; Emanuel K. Pecku; Joseph A. Awuni; Timothy S. Wallis; David W. Verner‐Jeffreys
Source:
Transboundary and emerging diseases 2021 v.68 no.3 pp. 1550-1563
ISSN:
1865-1674
Subject:
DNA; Infectious spleen and kidney necrosis virus; Nodaviridae; Streptococcus agalactiae; ascites; cage culture; chromatin; farms; histopathology; lakes; mortality; transmission electron microscopy; viruses; Ghana
Abstract:
... In late 2018, unusual patterns of very high mortality (>50% production) were reported in intensive tilapia cage culture systems across Lake Volta in Ghana. Samples of fish and fry were collected and analysed from two affected farms between October 2018 and February 2019. Affected fish showed darkening, erratic swimming and abdominal distension with associated ascites. Histopathological observation ...
DOI:
10.1111/tbed.13825

31. First report of the infectious spleen and kidney necrosis virus (ISKNV) infection in ornamental fishes in India

Author:
S. K. Girisha; K. B. Kushala; M. S. Nithin; T. G. Puneeth; B. T. Naveen Kumar; T. N. Vinay; T. Suresh; S. K. Ajay; M. N. Venugopal; K. S. Ramesh
Source:
Transboundary and emerging diseases 2021 v.68 no.2 pp. 964-972
ISSN:
1865-1674
Subject:
Infectious spleen and kidney necrosis virus; Japan; Lates calcarifer; biosecurity; cell lines; coat proteins; genes; ornamental fish; phylogeny; sequence homology; viruses; Australia; India; Malaysia
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV), a member of family iridoviridae, reported for the first time in a wide range of ornamental fish species in India. Significant mortalities during the year 2018–19 were reported from a number of retailers in the region with various clinical signs. The samples of moribund, dead and apparently healthy ornamental fishes were collected from retailers, ...
DOI:
10.1111/tbed.13793

32. Gefitinib inhibits infectious spleen and kidney necrosis virus infection in vivo and vitro by blocking virus endocytosis

Author:
Yinjie Niu; Xiaozhe Fu; Qiang Lin; Lihui Liu; Xia Luo; Hongru Liang; Ningqiu Li
Source:
Aquaculture 2021 v.539 pp. 736614
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; aquaculture; brain; dose response; endocytosis; fisheries; pathogenicity; phosphorylation; protein synthesis; viruses
Abstract:
... Infectious spleen and kidney necrosis virus disease (ISKNVD) caused significant economic losses to the fishery industry. So it is urgent to develop an effective measure for prevention ISKNVD. In this study, we investigated the effects of gefitinib against ISKNV in vitro and vivo. The gefitinib inhibited viral protein synthesis and decreased viral titers with the dose-dependent manner in Chinese pe ...
DOI:
10.1016/j.aquaculture.2021.736614

33. Identification, histopathology, and phylogenetic analysis of an iridovirus from cultivated silver pomfret in Zhejiang Province, East China

Author:
Si-Zhen Ni; Ya-Jun Wang; Jia-Bao Hu; Jian Shi; Yue Xu; Su-Ming Zhou; Jin-Jin Li; Bao-Hua Hong; Dong Qian
Source:
Aquaculture 2021 v.530 pp. 735619
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; Larimichthys crocea; Oxyeleotris marmorata; Pampus argenteus; adenosinetriphosphatase; ascites; blood platelets; breathing; coasts; coat proteins; erythrocytes; farmed fish; fins; fish diseases; fish farms; genes; genetic distance; gills; histopathology; kidneys; liver; lymphocytes; marine fish; monocytes; mortality; mucus; parasites; pathogens; phylogeny; spleen; summer; swimming; transmission electron microscopy; China; Japan; Taiwan
Abstract:
... Silver pomfret [Pampus argenteus (Euphrasén, 1788)] is a perciform fish that is widely distributed in coastal regions of the Indo-West Pacific, and is a favourite food species in China. A serious disease occurred in silver pomfret at a marine fish farm in Ningbo, Zhejiang Province, East China, in the summer of 2018, causing high fish mortality. Infected fish showed darkened skin, imbalanced swimmi ...
DOI:
10.1016/j.aquaculture.2020.735619

34. Infectious spleen and kidney necrosis virus‐associated large‐scale mortality in farmed giant gourami, Osphronemus goramy, in India

Author:
Thangaraj Raja Swaminathan; Nithianantham Sundar Raj; Prasannan Geetha Preena; Pravata Kumar Pradhan; Neeraj Sood; Rahul Girish Kumar; Arun Sudhagar; Naresh Kumar Sood
Source:
Journal of fish diseases 2021 v.44 no.12 pp. 2043-2053
ISSN:
0140-7775
Subject:
Gasterosteus aculeatus; Infectious spleen and kidney necrosis virus; Iridovirus; Osphronemus; Turbot reddish body iridovirus; aquaculture; biosecurity; cell lines; coat proteins; cytoplasm; freshwater; genes; kidneys; marine fish; mortality; necrosis; nucleocapsid; phylogeny; spleen; transmission electron microscopy; viruses; India; South East Asia
Abstract:
... Megalocytivirus cause diseases that have serious economic impacts on aquaculture, mainly in East and South‐East Asia. Five primary genotypes are known: infectious spleen and kidney necrosis virus (ISKNV), red sea bream iridovirus (RSIV), turbot reddish body iridovirus (TRBIV), threespine stickleback iridovirus (TSIV) and scale drop disease virus (SDDV). ISKNV‐mediated infectious spleen and kidney ...
DOI:
10.1111/jfd.13519

35. Isolation, identification and genomic analysis of an ISKNV-type megalocytivirus from spotted knifejaw (Oplegnathus punctatus)

Author:
Xiaohong Huang; Jingguang Wei; Qi Zheng; Ya Zhang; Weibin Zhu; Jiangchun Liu; Yunxia Hou; Qiwei Qin; Youhua Huang
Source:
Aquaculture 2021 v.532 pp. 736032
ISSN:
0044-8486
Subject:
DNA-directed DNA polymerase; Infectious spleen and kidney necrosis virus; Iridovirus; Oplegnathus; aquaculture; aquaculture industry; brain; coat proteins; cytopathogenicity; cytoplasm; cytoplasmic inclusions; disease outbreaks; electron microscopy; genes; genomics; kidneys; membrane proteins; mortality; open reading frames; pathogens; phylogeny; ribonucleases; spleen; virion; China
Abstract:
... The spotted knifejaw (Oplegnathus punctatus) is an emerging economical aquaculture species in China in recent years. In the present study, we characterized an iridovirus-like disease outbreak in spotted knifejaw aquaculture industry in Shandong Province (SKIV-SD), China. SKIV-SD induced severe cytopathic effect (CPE) in spotted knifejaw brain (SKB) cells as the infection proceeded. Moreover, elect ...
DOI:
10.1016/j.aquaculture.2020.736032

36. Molecular Identification and mRNA Expression Profiles of Galectin-9 Gene in Red Sea Bream (Pagrus major) Infected with Pathogens

Author:
Kwang-Min Choi; Min-Soo Joo; Dong-Hee Cho; Won-Sik Woo; Gyoungsik Kang; Min Jin Heo; Do-Hyung Kim; Chan-Il Park
Source:
Animals 2021 v.11 no.1 pp. -
ISSN:
2076-2615
Subject:
Edwardsiella piscicida; Infectious spleen and kidney necrosis virus; Pagrus major; Sparus aurata; Streptococcus iniae; carbohydrates; galectins; gene expression; genes; intestines; kidneys; ligands; pathogens; phylogeny; spleen
Abstract:
... Galectin (Gal) is a member of a family of β-galactoside-binding lectin. The members of this family play important roles in the recognition of carbohydrate ligands and in various other biological processes. In this study, we identified the gene encoding Gal-9 in Pagrus major (PmGal-9) and analyzed its expression in various tissues after pathogen challenge. Alignment analysis revealed that the two g ...
DOI:
10.3390/ani11010139

37. Molecular evidence for homologous strains of infectious spleen and kidney necrosis virus (ISKNV) genotype I infecting inland freshwater cultured Asian sea bass (Lates calcarifer) in Thailand

Author:
Pattarawit Kerddee; Nguyen Dinh‐Hung; Ha Thanh Dong; Ikuo Hirono; Chayanit Soontara; Nontawith Areechon; Prapansak Srisapoome; Pattanapon Kayansamruaj
Source:
Archives of virology 2021 v.166 no.11 pp. 3061-3074
ISSN:
0304-8608
Subject:
Infectious spleen and kidney necrosis virus; Lates calcarifer; adenosinetriphosphatase; apoptosis; fish; freshwater; genotype; hemorrhage; kidneys; liver; metagenomics; mortality; sequence analysis; vaccines; virology; viruses; Thailand
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) is a fish-pathogenic virus belonging to the genus Megalocytivirus of the family Iridoviridae. In 2018, disease occurrences (40-50% cumulative mortality) associated with ISKNV infection were reported in grown-out Asian sea bass (Lates calcarifer) cultured in an inland freshwater system in Thailand. Clinical samples were collected from seven distin ...
DOI:
10.1007/s00705-021-05207-7

38. Multiplex PCR method for distinguishing two genotypes of Megalocytivirus isolates in Korea

Author:
Hanchang Sohn; Seongdo Lee; Hyukjae Kwon; Mun Gyeong Kwon; Jee Youn Hwang; Seong Don Hwang; Jehee Lee
Source:
Aquaculture reports 2021 v.21 pp. 100886
ISSN:
2352-5134
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; Oplegnathus fasciatus; Pagrus major; Turbot reddish body iridovirus; World Organization for Animal Health; adenosinetriphosphatase; aquaculture; aquaculture industry; bream; detection limit; mortality; plasmids; polymerase chain reaction; viruses; Korean Peninsula
Abstract:
... Megalocytivirus is a genus of piscine viruses that belongs to the Iridoviridae family, and this family includes red seabream iridovirus (RSIV), turbot reddish body iridovirus (TRBIV), and infectious spleen and kidney necrosis virus. RSIV causes high mortality and economic losses in the rock bream (Oplegnathus fasciatus) Korean aquaculture industry. The World Organization for Animal Health’s Office ...
DOI:
10.1016/j.aqrep.2021.100886

39. Outbreak investigation attributes Infectious spleen and kidney necrosis virus as a necessary cause of a mortality epidemic in farmed grouper (Epinephelus spp.) in Bali, Indonesia

Author:
Fusianto Cahya; Paul M. Hick; Murwantoko; Afri Herlambang; Richard J. Whittington; Joy A. Becker
Source:
Aquaculture reports 2021 v.20 no. pp. 100723
ISSN:
2352-5134
Subject:
Betanodavirus; DNA; Epinephelus; Infectious spleen and kidney necrosis virus; Monogenea; coasts; ectoparasites; grouper; histopathology; hybrids; liver; metagenomics; microbiology; mortality; necropsy; necrosis; outbreak investigation; Indonesia
Abstract:
... In 2016, famers reported mass mortality events in hybrid grouper within 2–4 weeks following transfer to sea cages on the northern coast of Bali. The objective was to obtain an aetiological diagnosis using a broad range of traditional and emerging diagnostic approaches. A group of 24 and 12 fish with and without clinical signs were sampled from 10 affected populations at 9 farms. Samples for histop ...
DOI:
10.1016/j.aqrep.2021.100723

40. Pathogenicity and histopathology of infectious spleen and kidney necrosis virus genotype II (ISKNV-II) recovering from mass mortality of farmed Asian seabass, Lates calcarifer, in Southern China

Author:
Zhiming Zhu; Chao Duan; Yong Li; Congling Huang; Shaoping Weng; Jianguo He; Chuanfu Dong
Source:
Aquaculture 2021 v.534 pp. 736326
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; Lates calcarifer; fluorescent antibody technique; freshwater; genotype; histopathology; immunohistochemistry; kidneys; liver; marine fish; morbidity; mortality; pathogenicity; pathogens; spleen; transmission electron microscopes; viruses; China
Abstract:
... Megalocytivirus of family Iridoviridae was considered as one of the most alarming causative agents which caused fatally systemic infection and lead to mass mortalities to world-widely distributed freshwater and marine fish. In recent years, severe diseases with typical symptoms of body blackened, swims slowly to the water surface or surrounding the ponds were observed frequently in cultured Asian ...
DOI:
10.1016/j.aquaculture.2020.736326

41. Simultaneous detection of multiple bacterial and viral aquatic pathogens using a fluorogenic loop‐mediated isothermal amplification‐based dual‐sample microfluidic chip

Author:
Qian‐Jin Zhou; Jian‐Fei Lu; Xiu‐Rong Su; Jing‐Lei Jin; Shang‐Yang Li; Yan Zhou; Lei Wang; Xin‐Bin Shao; Yao‐Hua Wang; Mao‐Cang Yan; Ming‐Yun Li; Jiong Chen
Source:
Journal of fish diseases 2021 v.44 no.4 pp. 401-413
ISSN:
0140-7775
Subject:
Aeromonas hydrophila; Edwardsiella tarda; Infectious spleen and kidney necrosis virus; Vibrio harveyi; White spot syndrome virus; aquaculture; automation; early diagnosis; fish; loop-mediated isothermal amplification; necrosis; organ-on-a-chip; pathogens; plasmids; viruses
Abstract:
... Rapid and user‐friendly diagnostic tests are necessary for early diagnosis and immediate detection of diseases, particularly for on‐site screening of pathogenic microorganisms in aquaculture. In this study, we developed a dual‐sample microfluidic chip integrated with a real‐time fluorogenic loop‐mediated isothermal amplification assay (dual‐sample on‐chip LAMP) to simultaneously detect 10 pathogen ...
DOI:
10.1111/jfd.13325

42. Single-walled carbon nanotubes as drug carrier loaded with moroxydine hydrochloride against infectious spleen and kidney necrosis virus in mandarin fish

Author:
Zhao Zhao; Rui Ma; Zi-Rao Guo; Chen Zhang; Yan Xiong; Gao-Xue Wang; Bin Zhu
Source:
Aquaculture 2021 v.536 pp. 736469
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; antiviral properties; aquaculture; carbon nanotubes; complement; digestive tract; drug carriers; drugs; enzymes; fish; mortality
Abstract:
... Infectious diseases of viral origin cause major aquatic production losses in different parts of the world. There are lots of limited therapeutic effects for large amounts of antiviral drugs because of several impenetrable barriers (cell, skin, and gastrointestinal tract). In this study, we selected moroxydine hydrochloride (Mor) for the treatment of infectious spleen and kidney necrosis virus (ISK ...
DOI:
10.1016/j.aquaculture.2021.736469

43. Transcriptional and subcellular characterization of interferon induced protein-35 (IFP35) in mandarin fish, Siniperca chuatsi

Author:
Li Li; Shan Nan Chen; Nan Li; P. Nie
Source:
Developmental and comparative immunology 2021 v.115 pp. 103877
ISSN:
0145-305X
Subject:
DNA; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; cytoplasm; exons; fish; gene expression; immune system; innate immunity; introns; leucine zipper; luciferase; polyinosinic-polycytidylic acid; protein content; transcription (genetics)
Abstract:
... Interferon (IFN)-stimulated genes (ISGs) exert multiple functions in immune system, and IFN-induced protein 35 (IFP35), which is a member of ISG, has been suggested to be involved in numerous cellular activities including the regulation of antiviral immunity in mammals. However, the role of IFP35 in fish innate immunity remains largely unknown. In the present study, we characterized the IFP35 gene ...
DOI:
10.1016/j.dci.2020.103877

44. Transcriptomic and proteomic analyses of the immune mechanism in pathogenetic and resistant mandarin fish (Siniperca chuatsi) infected with ISKNV

Author:
Ying-Ying Wang; Yu Zhou; Huang-Cui Fu; He-Zhong Huang; Ze Li; Rui-Ming Jin; Xiao-Zhe Fu; Ning-Qiu Li
Source:
Aquaculture 2021 v.545 pp. 737198
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; coagulation; complement; cytotoxicity; eosin; fish; fish culture; gene expression regulation; histology; inflammation; leukocytes; pathogenicity; pathogens; programmed cell death; proteome; proteomics; spleen; transcriptome; transcriptomics; China
Abstract:
... Mandarin fish (Siniperca chuatsi) is one of the most economically important aquaculture species in China. Mandarin fish aquaculture has experienced major losses in the past few decades because of outbreaks of infectious spleen and kidney necrosis virus (ISKNV). Little is known about the immune mechanism of pathogenetic and resistant mandarin fish infected with ISKNV. Here, the transcriptome and pr ...
DOI:
10.1016/j.aquaculture.2021.737198

45. Co-infection of infectious spleen and kidney necrosis virus and Francisella sp. in farmed pearl gentian grouper (♀Epinephelus fuscoguttatus ×♂E. lanceolatus) in China - A case report

Author:
Yucong Huang; Shuanghu Cai; Jichang Jian; Guangfeng Liu; Liwen Xu
Source:
Aquaculture 2020 v.526 pp. 735409
ISSN:
0044-8486
Subject:
Francisella; Infectious spleen and kidney necrosis virus; Iridovirus; Maccullochella peelii peelii; anorexia; case studies; females; fish farms; gills; grouper; histopathology; macrophages; males; mixed infection; mortality; phylogeny; polymerase chain reaction; sequence analysis; signs and symptoms (animals and humans); splenomegaly; swimming; viruses; China
Abstract:
... From October–December 2018, a mass mortality among cultured pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂E. lanceolatus) occurred on a private grouper farm in Zhanjiang City, Guangdong Province, China. Approximately 120 dead fish from each pond were recorded daily, and the cumulative mortality was up to 52% within 56 days. Diseased fish showed clinical signs such as lethargy, anorexia, slo ...
DOI:
10.1016/j.aquaculture.2020.735409

46. Current use and development of fish vaccines in China

Author:
Qingchao Wang; Wei Ji; Zhen Xu
Source:
Fish & shellfish immunology 2020 v.96 pp. 223-234
ISSN:
1050-4648
Subject:
Aeromonas hydrophila; Ctenopharyngodon idella; Edwardsiella tarda; Infectious spleen and kidney necrosis virus; Paralichthys olivaceus; Vibrio alginolyticus; antibodies; aquaculture industry; ethics; farmed fish; field experimentation; fish production; food safety; immune response; inactivated vaccines; live vaccines; markets; shellfish; sustainable development; turbot; vaccination; veterinary medicine; China
Abstract:
... In the past decades, the aquaculture industry made great progress in China, which contributes more than 70% yield of the world's farmed fish. Along with the rapid growth of fish production, increased emergence and outbreak of numbers of diseases pose harm to the aquaculture industry and food safety. From the efficient, safe, environmental and ethical aspects, vaccines is definitely the most approp ...
DOI:
10.1016/j.fsi.2019.12.010

47. Development and application of a sensitive droplet digital PCR (ddPCR) for the detection of infectious spleen and kidney necrosis virus

Author:
Qiang Lin; Xiaozhe Fu; Lihui Liu; Hongru Liang; Yinjie Niu; Yuanya Wen; Zhibin Huang; Ningqiu Li
Source:
Aquaculture 2020 v.529 pp. 735697
ISSN:
0044-8486
Subject:
DNA; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; detection limit; droplets; fish; fish industry; kidneys; monitoring; necrosis; quantitative polymerase chain reaction; spleen; viral load
Abstract:
... Infectious spleen and kidney necrosis, caused by the infectious spleen and kidney necrosis virus (ISKNV), has damaged the economy of the world fish industry. Droplet digital PCR (ddPCR) is a novel, sensitive, accurate and absolute quantitation method that does not require a standard curve. In this study, we established a sensitive ddPCR method to rapidly detect and quantify ISKNV DNA. The establis ...
DOI:
10.1016/j.aquaculture.2020.735697

48. Development of a high-dose vaccine formulation for prevention of megalocytivirus infection in rock bream (Oplegnathus fasciatus)

Author:
Woo Ju Kwon; Jae Chan Choi; Suhee Hong; Young Chul Kim; Min Gyeong Jeong; Joon Gyu Min; Joon Bum Jeong; Kwang Il Kim; Hyun Do Jeong
Source:
Vaccine 2020 v.38 no.51 pp. 8107-8115
ISSN:
0264-410X
Subject:
Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Pagrus major; adjuvants; aluminum hydroxide; antigens; bream; cell lines; dose response; mortality; squalene; water temperature
Abstract:
... A formalin-inactivated red sea bream iridovirus (RSIV) vaccine was prepared using the culture supernatant of a persistently infected Pagrus major fin cell line (PI-PMF) with IVS-1 strain (RSIV subtype II Meglaocytivirus). Rock bream (Oplegnathus fasciatus) were injected with a high-dose, ultracentrifuged megalocytivirus vaccine (Ultra HSCMV, 7.0 × 10¹⁰ copies/mL), a high-dose supernatant of cultur ...
DOI:
10.1016/j.vaccine.2020.11.001

49. EPA and DHA can modulate cell death via inhibition of the Fas/tBid-mediated signaling pathway with ISKNV infection in grouper fin cell line (GF-1) cells

Author:
Kuang-Wen Chen; Hsuan-Wen Chiu; Yu-Wei Chiu; Jen-Leih Wu; Jiann-Ruey Hong
Source:
Fish & shellfish immunology 2020 v.97 pp. 608-616
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; apoptosis; caspase-3; caspase-8; cell lines; cell membranes; cell viability; death; docosahexaenoic acid; eicosapentaenoic acid; fish diseases; gene expression regulation; genes; grouper; nucleotidyltransferases; signal transduction; viral load
Abstract:
... Polyunsaturated fatty acids (PUFAs) play important roles in organisms, including the structure and liquidity of cell membranes, anti-oxidation and anti-inflammation. Very little has been done in terms of the effect of PUFAs on cell death, especially on DNA virus. In this study, we demonstrated that the infectious spleen and kidney necrosis virus (ISKNV) can induce host cell death via the apoptotic ...
DOI:
10.1016/j.fsi.2019.10.029

50. Genomic and functional characterization of the lect2 gene from Siniperca chuatsi

Author:
Yawei Shen; Ming Cao; Shoujie Tang; Yan Zhao; Jinliang Zhao; Xiaowu Chen; Yanhui Bi
Source:
Fish & shellfish immunology 2020 v.107 pp. 146-155
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; artificial diets; brain; carnivores; chemoattractants; digestive physiology; domestication; fish; gene expression; gene expression regulation; genes; genomic islands; genomics; immunology; industrialization; intestines; kidneys; leukocytes; lipopolysaccharides; liver; methylation; muscles; polyinosinic-polycytidylic acid; promoter regions; shellfish; stomach; China
Abstract:
... Mandarin fish (Siniperca chuatsi) is an important economic fish in China. Viral and bacterial diseases seriously affect the artificial culture of S. chuatsi. As a carnivorous fish, artificial feed domestication is also an important means to improve the scale of S. chuatsi culture. Therefore, the study of immunology and digestive physiology is very important to the industrial development of S. chua ...
DOI:
10.1016/j.fsi.2020.09.034

51. Identification of giant gourami iridovirus (GGIV): a new infectious spleen and kidney necrosis virus (ISKNV) from natural outbreak in cultured Osphronemus goramy

Author:
Sukenda Sukenda; Lila Gardenia; M. Zairin Jr; Angela Lusiastuti; Alimuddin Alimudin
Source:
Aquaculture international 2020 v.28 no.3 pp. 1069-1082
ISSN:
0967-6120
Subject:
DNA; Infectious spleen and kidney necrosis virus; Iridovirus; Osphronemus; anorexia; coat proteins; financial economics; fish; fish diseases; food animals; genes; hemorrhage; histopathology; industry; intraperitoneal injection; kidneys; liver; mortality; pathogens; phylogeny; quantitative polymerase chain reaction; signs and symptoms (animals and humans); spleen; Indonesia
Abstract:
... Megalocytivirus of family Iridoviridae has been identified as a pathogen that caused the fatal systemic infection to lead to massive death of numerous fish species in both ornamental and food fish industries. It caused significant economic losses due to severe mortality of infected fish. Since 2011 in Indonesia, megalocytivirus in giant gourami (Osphronemus goramy) had been found as the main patho ...
DOI:
10.1007/s10499-020-00513-4

52. Identification of infectious spleen and kidney necrosis virus (ISKNV)-encoded microRNAs

Author:
Jian-hui He; Qiong Xia; Shaoping Weng; Jianguo He; Xiaopeng Xu
Source:
Virus genes 2020 v.56 no.6 pp. 724-733
ISSN:
0920-8569
Subject:
Infectious spleen and kidney necrosis virus; aquaculture; coat proteins; fish; gene expression; microRNA; prediction; quantitative polymerase chain reaction; viruses
Abstract:
... MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptionally regulate gene expression by complementary binding to target mRNAs. Virus-encoded miRNAs play important roles in virus life cycle and virus-host interactions. Viruses from the Megalocytivirus genus, family Iridoviridae, infect a wide range of fishes, bringing great challenges to aquaculture. Infectious spleen and kidney necrosi ...
DOI:
10.1007/s11262-020-01798-6

53. Identification of intron in ORF003 gene and its application for inactivation test of ISKNV

Author:
Xiaozhe Fu; Lixi Zhang; Lihui Liu; Qiang Lin; Hongru Liang; Yinjie Niu; Zhibin Huang; Ningqiu Li
Source:
Microbial pathogenesis 2020 v.138 pp. 103822
ISSN:
0882-4010
Subject:
DNA; DNA viruses; annealing; cost effectiveness; detection; fish; inactivated vaccines; introns; messenger RNA; oligodeoxyribonucleotides; reverse transcriptase polymerase chain reaction; sequence analysis; temperature; transcription (genetics); transcriptomics; viruses
Abstract:
... The virus inactivation test is a critical skill in inactivated vaccine production. Active viruses produced viral mRNA in susceptible cells or the host can be used to infer whether a DNA virus is replicating by RT-PCR. But it is generally difficult to avoid genomic DNA contamination in the samples. However, the use of primers spanning an intron is an effective alternative for virus inactivation tes ...
DOI:
10.1016/j.micpath.2019.103822

54. Impact of Aeromonas hydrophila and infectious spleen and kidney necrosis virus infections on susceptibility and host immune response in Chinese perch (Siniperca chuatsi)

Author:
Xiaodan Liu; Wei Sun; Yanbing Zhang; Yifan Zhou; Jingwen Xu; Xiaojian Gao; Shuangming Zhang; Xiaojun Zhang
Source:
Fish & shellfish immunology 2020 v.105 pp. 117-125
ISSN:
1050-4648
Subject:
Aeromonas hydrophila; Infectious spleen and kidney necrosis virus; Siniperca chuatsi; fish; hepcidin; histopathology; immune response; immune system; inflammation; mixed infection; mortality; secondary infection; shellfish; viral load
Abstract:
... Co-infections with pathogenic microorganisms are common in aquaculture, resulting in more serious economic losses than single-pathogen infection. Infection of Aeromonas hydrophila (A. hydrophila) often occurs together with infectious spleen and kidney necrosis virus (ISKNV) in Chinese perch (Siniperca chuatsi) culture ponds. In this study, A. hydrophila and ISKNV were inoculated into Chinese perch ...
DOI:
10.1016/j.fsi.2020.07.012

55. In vivo efficacy of ganciclovir by using single-walled carbon nanotubes as a drug carrier against infectious spleen and kidney necrosis virus in mandarin fish

Author:
Zhao Zhao; Chen Zhang; Yi-Jun Jia; Gao-Xue Wang; Bin Zhu
Source:
Aquaculture 2020 v.526 pp. 735434
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; antiviral agents; carbon nanotubes; complement; drug carriers; drugs; enzyme activity; fish; fish diseases; gastrointestinal system; genes; mortality; plant diseases and disorders; plant viruses
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) cause a high mortality disease which leads to a significant economic loss on mandarin fish in China. At present, there is no effective drug against this fatal disease because of several impenetrable barriers (cell, skin and gastrointestinal tract). In this study, we selected ganciclovir for the treatment of ISKNV and choose single-walled carbon n ...
DOI:
10.1016/j.aquaculture.2020.735434

56. Myxovirus resistance (Mx) gene and its differential expression regulated by three type I and two type II IFNs in mandarin fish, Siniperca chuatsi

Author:
Li Li; Shan Nan Chen; Zubair Ahmed Laghari; Hui Jun Huo; Jing Hou; Lin Huang; Nan Li; P. Nie
Source:
Developmental and comparative immunology 2020 v.105 pp. 103604
ISSN:
0145-305X
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; fish; gene expression regulation; genes; interferon-gamma; protein content; response elements; tissues; virus replication
Abstract:
... Interferons (IFNs) can induce the expression of IFN-stimulated genes (ISGs), such as myxovirus resistance (Mx) protein, to inhibit virus replication. In this study, the expression of Mx gene in mandarin fish, and the IFN-sensitive response elements (ISREs) and gamma-interferon activated sites (GASs) in the promoter of Mx gene were analyzed in relation to the stimulation of three distinct type I IF ...
DOI:
10.1016/j.dci.2019.103604

57. Novel peptide nucleic acid-based real-time PCR assay for detection and genotyping of Megalocytivirus

Author:
Eun Sun Lee; Miyoung Cho; Eun Young Min; Sung Hee Jung; Kwang Il Kim
Source:
Aquaculture 2020 v.518 pp. 734818
ISSN:
0044-8486
Subject:
DNA; Infectious spleen and kidney necrosis virus; Pagrus major; Turbot reddish body iridovirus; animal diseases; aquatic animals; coat proteins; detection limit; diagnostic techniques; etiological agents; fluorescence; genes; genotype; genotyping; hybridization; melting; melting point; nucleic acid hybridization; peptides; phylogeny; quantitative polymerase chain reaction; sequence analysis
Abstract:
... Megalocytivirus, the causative agent for Red Sea Bream Iridoviral Disease (RSIVD), is classified into three genotypes based on its phylogeny: Red Sea Bream Iridovirus (RSIV), Infectious Spleen and Kidney Necrosis Virus (ISKNV), and Turbot Reddish Body Iridovirus (TRBIV). To overcome drawbacks such as limitation of genotypic variant type discrimination and unexpected PCR reaction from an SYBR green ...
DOI:
10.1016/j.aquaculture.2019.734818

58. Optimization of the efficacy of a SWCNTs-based subunit vaccine against infectious spleen and kidney necrosis virus in mandarin fish

Author:
Zhao Zhao; Yan Xiong; Chen Zhang; Yi-Jun Jia; De-Kui Qiu; Gao-Xue Wang; Bin Zhu
Source:
Fish & shellfish immunology 2020 v.106 pp. 190-196
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; antibody formation; blood serum; enzymes; fish; fish culture; mortality; shellfish; subunit vaccines; vaccination; China
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) cause a high mortality disease which brings substantial economic losses to the mandarin fish culture industry in China. This study was aimed at optimizing the efficacy of a SWCNTs-based immersion subunit vaccine (SWCNTs-M-MCP) which as a promising vaccine against ISKNV. Mandarin fish were vaccinated by immersion, then we designed an orthogonal ex ...
DOI:
10.1016/j.fsi.2020.07.062

59. PCR Detection and Phylogenetic Analysis of Megalocytivirus Isolates in Farmed Giant Sea Perch Lates calcarifer in Southern Taiwan

Author:
Jia-Ming Tsai; Song-Lang Huang; Chung-Da Yang
Source:
Viruses 2020 v.12 no.6 pp. -
ISSN:
1999-4915
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; Lates calcarifer; Turbot reddish body iridovirus; World Organization for Animal Health; aquaculture industry; coat proteins; etiological agents; fingerlings; fish culture; freshwater fish; genes; genetic variation; genotype; host range; mortality; nucleotide sequences; oligodeoxyribonucleotides; perch; phylogeny; polymerase chain reaction; sequence analysis; viruses; South East Asia; Taiwan
Abstract:
... The Megalocytivirus genus includes three genotypes, red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis virus (ISKNV), and turbot reddish body iridovirus (TRBIV), and has caused mass mortalities in various marine and freshwater fish species in East and Southeast Asia. Of the three genotypes, TRBIV-like megalocytivirus is not included in the World Organization for Animal Health ( ...
DOI:
10.3390/v12060681

60. Rapid diagnosis of two marine viruses, red sea bream iridovirus and viral hemorrhagic septicemia virus by PCR combined with lateral flow assay

Author:
Haneul Seo; Eui-Joon Kil; Chairina Fadhila; Thuy Thi Bich Vo; Chung-Kyoon Auh; Taek-Kyun Lee; Sukchan Lee
Source:
Virusdisease 2020 v.31 no.3 pp. 251-256
ISSN:
2347-3584
Subject:
Infectious spleen and kidney necrosis virus; Paralichthys olivaceus; Sebastes schlegelii; Viral hemorrhagic septicemia virus; early diagnosis; fish; genome; mortality; pathogens; polymerase chain reaction; viruses; South Korea
Abstract:
... Red sea bream iridovirus (RSIV) and Viral hemorrhagic septicemia virus (VHSV) are the most important viral marine pathogens in South Korea because RSIV and VHSV infect and cause high mortality rates in major fish species such as Paralichthys olivaceus and Sebastes schlegelii. These viruses can be transmitted both vertically and horizontally, and early diagnosis is imperative. In this research, RSI ...
DOI:
10.1007/s13337-020-00577-z

61. Review of diseases and health management in zebrafish Danio rerio (Hamilton 1822) in research facilities

Author:
M. L. Kent; J. L. Sanders; S. Spagnoli; C. E. Al‐Samarrie; K. N. Murray
Source:
Journal of fish diseases 2020 v.43 no.6 pp. 637-650
ISSN:
0140-7775
Subject:
Coccidiomorphea; Danio rerio; Edwardsiella ictaluri; Infectious spleen and kidney necrosis virus; Mycobacterium chelonae; Mycobacterium haemophilum; Mycobacterium marinum; biomedical research; biosecurity; carcinoma; fish; inflammation; models; mycobacterial diseases; nephrocalcinosis; parasites; pathogens; pets; research facilities; risk; sarcoma; water quality
Abstract:
... The use of zebrafish (Danio rerio) in biomedical research has expanded at a tremendous rate over the last two decades. Along with increases in laboratories using this model, we are discovering new and important diseases. We review here the important pathogens and diseases based on some 20 years of research and findings from our diagnostic service at the NIH‐funded Zebrafish International Resource ...
DOI:
10.1111/jfd.13165

62. Single-walled carbon nanotubes as delivery vehicles enhance the immunoprotective effect of an immersion DNA vaccine against infectious spleen and kidney necrosis virus in mandarin fish

Author:
Zhao Zhao; Chen Zhang; Qiang Lin; Ning-qiu Li; Zhi-Bin Huang; Mi Zhao; Xiao-Zhe Fu; Gao-Xue Wang; Bin Zhu
Source:
Fish & shellfish immunology 2020 v.97 pp. 432-439
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; antibody formation; blood serum; carbon nanotubes; enzyme activity; fish; fish culture; fish industry; genes; immunization; juveniles; kidneys; mortality; muscles; recombinant vaccines; spleen; tissues; transcription (genetics); China
Abstract:
... As a high mortality disease, Infectious spleen and kidney necrosis virus (ISKNV) can cause massive economic damage on mandarin fish farming industry in China, which seriously hindered the development of mandarin fish farming industry. In this research, SWCNTs (single-walled carbon nanotubes) as a candidate for DNA vaccine carrier was vaccinated by immersion (1, 2, 5, 10, 20 mg/L) in juvenile manda ...
DOI:
10.1016/j.fsi.2019.12.072

63. Transcriptional programs of infectious spleen and kidney necrosis virus (ISKNV) in vitro and in vivo

Author:
Jian-hui He; Lichao Huang; Zhixun Guo; Shaoping Weng; Jianguo He; Xiaopeng Xu
Source:
Virus genes 2020 v.56 no.6 pp. 749-755
ISSN:
0920-8569
Subject:
Infectious spleen and kidney necrosis virus; freshwater; heat; quantitative polymerase chain reaction; transcription (genetics); viruses
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV), causing serious infectious diseases to marine and freshwater fishes, is the type species of the genus Megalocytivirus, family Iridoviridae. In this study, the transcriptional programs of ISKNV in vitro (MFF-1 cells) and in vivo (spleens from mandarin fish) were investigated using real-time PCR. Transcription of all the putative open reading fram ...
DOI:
10.1007/s11262-020-01800-1

64. The roles of mandarin fish STING in innate immune defense against Infectious spleen and kidney necrosis virus infections

Author:
Xiao-Wei Qin; Jian He; Yang Yu; Chang Liu; Zhi-Yong Luo; Zhi-Min Li; Shao-Ping Weng; Chang-Jun Guo; Jian-Guo He
Source:
Fish & shellfish immunology 2020 v.100 pp. 80-89
ISSN:
1050-4648
Subject:
DNA; Infectious spleen and kidney necrosis virus; RNA; Siniperca chuatsi; endoplasmic reticulum; enzymes; fish fry; fluorescent antibody technique; freshwater fish; gene overexpression; genes; immune response; innate immunity; interferons; luciferin; mutation; pathogens; serine
Abstract:
... The mandarin fish Siniperca chuatsi is a cultured freshwater fish species that is popular in China because of its high market value. With the development of high-density cultural mode in mandarin fish, viral diseases such as Infectious spleen and kidney necrosis virus (ISKNV) are becoming increasingly serious. Stimulator of interferon genes (STING) is a central component in the innate immune respo ...
DOI:
10.1016/j.fsi.2020.02.062

65. Characterization of gene expression profiles and functional analysis of peptidoglycan recognition protein 2 from rock bream (Oplegnathus fasciatus)

Author:
Kwang-Min Choi; Min-Soo Joo; Dong Hee Cho; Jin-Sol Bae; Ji-Min Jung; Jee Youn Hwang; Mun-Gyeong Kwon; Jung Soo Seo; Seong Don Hwang; Bo-Yeong Jee; Do-Hyung Kim; Chan-Il Park
Source:
Fish & shellfish immunology 2019 v.84 pp. 1068-1074
ISSN:
1050-4648
Subject:
Edwardsiella piscicida; Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Streptococcus iniae; amino acids; antimicrobial properties; bacteria; bacterial growth; bacterial infections; bream; cell membranes; complementary DNA; enzymes; gene expression; high-throughput nucleotide sequencing; intestines; liver; open reading frames; peptidoglycans; phagocytosis; phylogeny; protein domains; zinc
Abstract:
... Peptidoglycan recognition protein 2 (PGRP2) is a Zn2+-dependent peptidase that plays important roles in binding to microbial components of the cell membrane, inducing phagocytosis and antimicrobial activity. Rock bream (Oplegnathus fasciatus) PGRP2 (RbPGRP2) was identified in the intestine by next generation sequencing (NGS) analysis. The open reading frame (ORF) the RbPGRP2 cDNA (470 amino acid r ...
DOI:
10.1016/j.fsi.2018.11.025

66. Chitosan and anisodamine improve the immune efficacy of inactivated infectious spleen and kidney necrosis virus vaccine in Siniperca chuatsi

Author:
Jiacheng Zhang; Xiaozhe Fu; Yanqi Zhang; Wentao Zhu; Yong Zhou; Gailing Yuan; Xiaoling Liu; Taoshan Ai; Lingbing Zeng; Jianguo Su
Source:
Fish & shellfish immunology 2019 v.89 pp. 52-60
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; acid phosphatase; blood serum; catalase; chitosan; fish; immune response; infectious diseases; liver; messenger RNA; mortality; protective effect; reverse transcriptase polymerase chain reaction; spleen; tissues; tumor necrosis factor-alpha; urea nitrogen; vaccination; vaccine adjuvants; vaccines; virus replication
Abstract:
... Siniperca chuatsi is an economically important fish in China, but infectious spleen and kidney necrosis virus (ISKNV) causes high mortality and significant economic losses. Currently, vaccination is the most promising strategy to prevent infectious diseases, while adjuvant can effectively enhance immune responses. In this study, inactivated ISKNV vaccine was prepared, then poly (I:C), chitosan, an ...
DOI:
10.1016/j.fsi.2019.03.040

67. Data on molecular characterisation and expression analysis of the interferon-related developmental regulator 2 (IFRD2) gene from red sea bream, Pagrus major

Author:
Kyung Ho Kim; Andre Kim; Chan-Il Park
Source:
Data in Brief 2019 v.25 pp. 104142
ISSN:
2352-3409
Subject:
Edwardsiella piscicida; Infectious spleen and kidney necrosis virus; Pagrus major; Streptococcus iniae; adults; amino acid sequences; brain; gastrulation; genes; heart; hematopoiesis; immune response; kidneys; liver; lungs; myoblasts; phylogeny; placenta; skeletal muscle
Abstract:
... The interferon-related developmental regulator 1 (IFRD1) protein is expected to play a role in the regulation of inflammatory responses in adult mice, since it is known to repress transcription of NF-κB in myoblasts that regenerate skeletal muscle after traumatic injury Micheli et al., 2011. The IFRD2 gene is expressed in many tissues including skeletal muscle, kidney, heart, brain, lung, placenta ...
DOI:
10.1016/j.dib.2019.104142

68. Data on molecular characterization and expression of the C-reactive protein (CRP) gene from rock bream, Oplegnathus fasciatus

Author:
Kyung Ho Kim; Do-Hyung Kim; Chan-Il Park
Source:
Data in Brief 2019 v.26 pp. 104506
ISSN:
2352-3409
Subject:
C-reactive protein; Edwardsiella piscicida; Infectious spleen and kidney necrosis virus; Oplegnathus fasciatus; Streptococcus iniae; bream; gene expression; genes; inflammation; innate immunity; kidneys; nucleotide sequences; phylogeny; spleen
Abstract:
... C-reactive protein (CRP) is a member of the pentraxin family and is an acute-phase response to plasma protein; its level in blood increases rapidly in response to trauma, inflammation, and infection. In the present study, we analysed the molecular characteristics of the Oplegnathus fasciatus CRP (RbCRP) gene sequence using multiple alignments and phylogenetic analyses of the deduced amino acids. I ...
DOI:
10.1016/j.dib.2019.104506

69. Data on molecular characterization and expression profiles of the NF-κB repressing factor gene in red sea bream (Pagrus major)

Author:
Kwang-Min Choi; Jee Youn Hwang; Mun-Gyeong Kwon; Ji-Min Jeong; Jung Soo Seo; Seong Don Hwang; Bo-Yeong Jee; Mu-Chan Kim; Chan-Il Park
Source:
Data in Brief 2019 v.24 pp. 103977
ISSN:
2352-3409
Subject:
Infectious spleen and kidney necrosis virus; Pagrus major; Streptococcus iniae; amino acids; gene expression; genes; kidneys; liver; spleen; transcription (genetics); transcription factor NF-kappa B
Abstract:
... Nuclear factor-kappaB (NF-κB) repressing factor (NKRF) specifically inhibits the transcriptional activity of NF-κB protein. The PmNKRF cDNA is composed of 757 amino acid residues. Alignment analysis revealed that the G-patch and R3H domains are conserved in different organisms. We aimed to analyse red sea bream NKRF (PmNKRF) gene expression after infection with pathogens [Streptococcus iniae or re ...
DOI:
10.1016/j.dib.2019.103977

70. Data on molecular characterization and gene expression analysis of secretory carrier-associated membrane protein 5 (SCAMP5) from the red sea bream (Pagrus major)

Author:
Min Jin Heo; Andre Kim; Chan-Il Park
Source:
Data in Brief 2019 v.25 pp. 103901
ISSN:
2352-3409
Subject:
Edwardsiella piscicida; Infectious spleen and kidney necrosis virus; Pagrus major; Streptococcus iniae; cytokines; gene expression; genes; immune response; macrophages; membrane proteins; monocytes; phylogeny; secretion
Abstract:
... Secretory carrier membrane proteins (SCAMPs) are widely distributed integral membrane proteins implicated in membrane trafficking. Secretory carrier membrane protein 5 (SCAMP5) is expected to be involved in regulation of the immune response because it is expressed in a variety of immune tissues and promotes the secretion of cytokines in monocytes and macrophages. In this study, we performed an ana ...
DOI:
10.1016/j.dib.2019.103901

71. Deletion of the Infectious spleen and kidney necrosis virus ORF069L reduces virulence to mandarin fish Siniperca chuatsi

Author:
Yi-Fan Lin; Jian He; Ruo-Yun Zeng; Zhi-Min Li; Zhi-Yong Luo; Wei-Qiang Pan; Shao-Ping Weng; Chang-Jun Guo; Jian-Guo He
Source:
Fish & shellfish immunology 2019 v.95 pp. 328-335
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Retroviridae; Siniperca chuatsi; T-lymphocytes; amino acids; carcinogenesis; cell proliferation; death; fish; fish diseases; fruit diseases; gene deletion; genes; growth curves; homologous recombination; humans; immunoglobulin M; interleukin-1beta; interleukin-8; live vaccines; terminal repeat sequences; viral load; virulence; viruses; China
Abstract:
... Mandarin fish (Siniperca chuatsi) is a significant cultured species with high added value in China. With the expansion of farming, diseases of mandarin fish such as Infectious spleen and kidney necrosis virus (ISKNV) diseases are becoming more and more serious. Human endogenous retrovirus subfamily H long terminal repeat associating protein 2 (HHLA2) is a type 1 transmembrane molecule with three e ...
DOI:
10.1016/j.fsi.2019.10.039

72. First report of megalocytivirus (iridoviridae) in cultured bluegill sunfish, Lepomis macrochirus, in China

Author:
Lihui Liu; Lujun Yu; Xiaozhe Fu; Qiang Lin; Hongru Liang; Yinjie Niu; Ningqiu Li
Source:
Microbial pathogenesis 2019 v.135 pp. 103617
ISSN:
0882-4010
Subject:
Infectious spleen and kidney necrosis virus; Lepomis macrochirus; Siniperca chuatsi; aquaculture industry; brain; coat proteins; cytopathogenicity; electron microscopy; fish; genes; histopathology; intestines; liver; mortality; nucleotide sequences; pathogens; phylogeny; polymerase chain reaction; sequence alignment; spleen; taste; viral load; viruses; China
Abstract:
... The bluegill sunfish, Lepomis macrochirus, is an important aquacultural and recreational species in southern China because of its excellent taste, rapid growth rate, and good looks. At present, few pathogens are known to affect the bluegill sunfish. However, an iridovirus-like disease recently caused heavy losses to the bluegill sunfish aquaculture industry in Guangdong, China. We report that a vi ...
DOI:
10.1016/j.micpath.2019.103617

73. Functional characterization of IL-10 and its receptor subunits in a perciform fish, the mandarin fish, Siniperca chuatsi

Author:
Hui Jun Huo; Shan Nan Chen; Li Li; Pin Nie
Source:
Developmental and comparative immunology 2019 v.97 pp. 64-75
ISSN:
0145-305X
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; binding sites; cysteine; fish; gene expression; interleukin-10; interleukin-1beta; interleukin-6; interleukin-8; kidneys; mammals; receptors; signal transduction; spleen; splenocytes; tissues; tumor necrosis factor-alpha
Abstract:
... Interleukin (IL)-10 is an immune-regulatory cytokine with multiple functions. In the current study, IL-10 and its two receptors, IL-10R1 and IL-10R2 were identified in mandarin fish, Siniperca chuatsi. The inhibitory effect of mandarin fish IL-10 was investigated on pro-inflammatory cytokine expression and the ligand-receptor relationship. This IL-10 possesses conserved cysteine residues, predicte ...
DOI:
10.1016/j.dci.2019.03.017

74. Genetic diversity of Megalocytivirus from cultured fish in Korea

Author:
Kwang Il Kim; Eun Sun Lee; Jeong Wan Do; Seong Don Hwang; Miyoung Cho; Sung Hee Jung; Bo Young Jee; Woo Ju Kwon; Hyun Do Jeong
Source:
Aquaculture 2019 v.509 pp. 16-22
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; Lateolabrax japonicus; Platichthys stellatus; Siniperca scherzeri; Stephanolepis cirrhifer; Turbot reddish body iridovirus; bass; coat proteins; farmed fish; freshwater fish; genes; genetic variation; genotype; marine fish; mullet; sequence analysis; Korean Peninsula
Abstract:
... Since the first outbreak of Megalocytivirus infection in cultured marine fish in the 1990s, the Red sea bream iridovirus (RSIV) subtype II and Turbot reddish body iridovirus (TRBIV) type Megalocytivirus have been prevalent in Korea. Based on the major capsid protein (MCP) genes, the RSIV subtype II, as the predominant genotype, was identified in marine and freshwater fish including thread-sail fil ...
DOI:
10.1016/j.aquaculture.2019.05.014

75. Identification of infectious spleen and kidney necrosis virus from farmed barramundi Lates calcarifer in Thailand and study of its pathogenicity

Author:
Kittipong Thanasaksiri; Ryoko Takano; Kohei Fukuda; Tidaporn Chaweepack; Janenuj Wongtavatchai
Source:
Aquaculture 2019 v.500 pp. 188-191
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; Lates calcarifer; Pagrus major; coat proteins; disease control; dose response; emerging diseases; fluorescent antibody technique; genotype; kidneys; marine fish; monoclonal antibodies; mortality; necrosis; phylogeny; spleen; vaccines; virulence; Thailand
Abstract:
... Infectious spleen and kidney necrosis disease (ISKND) is one of major viral diseases in marine fish species. Here, we isolated infectious spleen and kidney necrosis virus (ISKNV) from diseased barramundi in Thailand and evaluated its pathogenicity with intramuscular challenge in naïve barramundi. ISKNV infection in grunt fin (GF) cells was observed within 14 days post inoculation (dpi) by immunofl ...
DOI:
10.1016/j.aquaculture.2018.09.035

76. Immersion vaccination of Mandarin fish Siniperca chuatsi against infectious spleen and kidney necrosis virus with a SWCNTs-based subunit vaccine

Author:
Zhao Zhao; Chen Zhang; Yi-Jun Jia; De-Kui Qiu; Qiang Lin; Ning-Qiu Li; Zhi-Bin Huang; Xiao-Zhe Fu; Gao-Xue Wang; Bin Zhu
Source:
Fish & shellfish immunology 2019 v.92 pp. 133-140
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; antibody formation; blood serum; carbon nanotubes; enzyme activity; fish; gastrointestinal system; genes; juveniles; mortality; protective effect; subunit vaccines; vaccination
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) cause a high mortality disease which lead to significant economic loss on mandarin fish in China. There is no effective drug or vaccine against this fatal disease at present. Meanwhile, many drugs and vaccines had no effect in many cases account of several impenetrable barriers (cell, skin and gastrointestinal tract). Here we reported an immersio ...
DOI:
10.1016/j.fsi.2019.06.001

77. Molecular cloning of Y-Box binding protein-1 from mandarin fish and its roles in stress-response and antiviral immunity

Author:
Jian He; Tao-Lin Xie; Xiao Li; Yang Yu; Zhi-Peng Zhan; Shao-Ping Weng; Chang-Jun Guo; Jian-Guo He
Source:
Fish & shellfish immunology 2019 v.93 pp. 406-415
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Rhabdoviridae; Siniperca chuatsi; amino acids; binding proteins; coat proteins; cold; cold shock proteins; cold stress; complementary DNA; enzymes; farmed fish; fish fry; gene expression regulation; gene overexpression; genes; innate immunity; luciferin; mammals; molecular cloning; open reading frames; plasmids; signal transduction; small interfering RNA; stress response; tissue distribution; tissues; transcription factor NF-kappa B; viruses
Abstract:
... Mandarin fish (Siniperca chuatsi) is a universally farmed fish species in China and has a large farming scale and economic value. With the high-density cultural mode in mandarin fish, viral diseases, such as infectious spleen and kidney necrosis virus (ISKNV) and Siniperca chuatsi rhabdovirus (SCRV), have increased loss, which has seriously restricted the development of aquaculture. Y-Box binding ...
DOI:
10.1016/j.fsi.2019.07.069

78. Stability of Infectious spleen and kidney necrosis virus and susceptibility to physical and chemical disinfectants

Author:
Cahya Fusianto; Paul M. Hick; Joy A. Becker
Source:
Aquaculture 2019 v.506 pp. 104-111
ISSN:
0044-8486
Subject:
DNA; Infectious spleen and kidney necrosis virus; Iridovirus; Maccullochella peelii peelii; Pagrus major; World Organization for Animal Health; aquaculture; aquariums; benzalkonium chloride; bioassays; biosecurity; cell culture; cellulose; certification; cost effectiveness; detection limit; disease control; disinfectants; disinfection; fetal bovine serum; fisheries; heat; host range; intraperitoneal injection; juveniles; ornamental fish; pH; pathogenicity; quantitative polymerase chain reaction; risk; signs and symptoms (animals and humans); sodium hypochlorite; tanks; Australia
Abstract:
... The genus Megalocytivirus (MCV) includes Infectious spleen and kidney necrosis virus (ISKNV) and red seabream iridovirus (RSIV) which are listed by the World Organization for Animal Health (OIE). MCV has a broad host range and can be present as a persistent subclinical infection in some fish. Australia is considered free from MCV and important aquaculture and wild fisheries are at risk. Consequent ...
DOI:
10.1016/j.aquaculture.2019.03.024

79. Viral infections in tilapines: More than just tilapia lake virus

Author:
Vimbai Irene Machimbirike; Mona Dverdal Jansen; Saengchan Senapin; Pongsak Khunrae; Triwit Rattanarojpong; Ha Thanh Dong
Source:
Aquaculture 2019 v.503 pp. 508-518
ISSN:
0044-8486
Subject:
Betanodavirus; Bohle iridovirus; Herpesviridae; Infectious pancreatic necrosis virus; Infectious spleen and kidney necrosis virus; Iridovirus; Lymphocystivirus; Tilapia lake virus; disease outbreaks; disease surveillance; mortality; pathogens; viruses
Abstract:
... There is an increasing number of emerging infectious agents, although not zoonotic, that affect farmed tilapines worldwide. Most recently, the emergence of a new virus, tilapia lake virus (TiLV), has diverted global attention to it while little or no research is being done on the other viruses of possible equal importance. These other viruses have reportedly been associated with relatively high mo ...
DOI:
10.1016/j.aquaculture.2019.01.036

80. The atypical chemokine receptor 4 in red sea bream (Pagrus major): Molecular characterization and gene expression analysis

Author:
Kwang-Min Choi; Seong Don Hwang; Min Soo Joo; Jee Youn Hwang; Mun-Gyeong Kwon; Ji-Min Jeong; Jung Soo Seo; Bo-Yeong Jee; Chan-Il Park
Source:
Fish & shellfish immunology 2019 v.93 pp. 50-54
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Pagrus major; Streptococcus iniae; amino acid sequences; chemokine receptors; chemokines; complementary DNA; cysteine; fish; gene expression; gene expression regulation; high-throughput nucleotide sequencing; immune response; immune system; kidneys; messenger RNA; nucleotide sequences; phylogeny
Abstract:
... Atypical chemokine receptor 4 (ACKR4) is regulated by cytokines, binds chemokines and regulates the chemokine gradient. We verified the cDNA sequence by confirming ACKR4 from red sea bream (PmACKR4) by next generation sequencing (NGS) and analysed the molecular characteristics and gene expression profile. In the analysis using the predicted amino acid sequence of PmACKR4, a highly conserved G prot ...
DOI:
10.1016/j.fsi.2019.07.004

81. The impact of pooling samples on surveillance sensitivity for the megalocytivirus Infectious spleen and kidney necrosis virus

Author:
Sophia J. Johnson; Paul M. Hick; Andrew P. Robinson; Anneke E. Rimmer; Alison Tweedie; Joy A. Becker
Source:
Transboundary and emerging diseases 2019 v.66 no.6 pp. 2318-2328
ISSN:
1865-1674
Subject:
Infectious spleen and kidney necrosis virus; detection limit; diagnostic sensitivity; emerging diseases; fish industry; hosts; import policies; monitoring; ornamental fish; pathogens; plasmids; quantitative polymerase chain reaction; screening; tissues; viral load; Australia
Abstract:
... Movements of large volumes and species varieties make the ornamental fish industry a high‐risk pathway for the transfer of aquatic pathogens to new geographical regions and naïve hosts, potentially resulting in emergency disease events. Infectious spleen and kidney necrosis virus (genus Megalocytivirus) is considered exotic to Australia despite documented incursions since 2003. There are current i ...
DOI:
10.1111/tbed.13288

82. Antibody profiling using a recombinant protein–based multiplex ELISA array accelerates recombinant vaccine development: Case study on red sea bream iridovirus as a reverse vaccinology model

Author:
Tomomasa Matsuyama; Natsumi Sano; Tomokazu Takano; Takamitsu Sakai; Motoshige Yasuike; Atushi Fujiwara; Yasuhiko Kawato; Jun Kurita; Kazunori Yoshida; Yukinori Shimada; Chihaya Nakayasu
Source:
Vaccine 2018 v.36 no.19 pp. 2643-2649
ISSN:
0264-410X
Subject:
Infectious spleen and kidney necrosis virus; Seriola dumerili; Seriola quinqueradiata; antibodies; antigens; blood serum; case studies; enzyme-linked immunosorbent assay; fish; genes; immune response; models; pathogens; prediction; protective effect; recombinant vaccines; screening; sorbents; vaccine development
Abstract:
... Predicting antigens that would be protective is crucial for the development of recombinant vaccine using genome based vaccine development, also known as reverse vaccinology. High-throughput antigen screening is effective for identifying vaccine target genes, particularly for pathogens for which minimal antigenicity data exist. Using red sea bream iridovirus (RSIV) as a research model, we developed ...
DOI:
10.1016/j.vaccine.2018.03.059

83. Black rockfish C-type lectin, SsCTL4: A pattern recognition receptor that promotes bactericidal activity and virus escape from host immune defense

Author:
Du Xue; Wang Guang-hua; Su Yan-li; Zhang Min; Hu Yong-hua
Source:
Fish & shellfish immunology 2018 v.79 pp. 340-350
ISSN:
1050-4648
Subject:
Edwardsiella tarda; Gram-positive bacteria; Infectious spleen and kidney necrosis virus; Sebastes melanops; Sebastes schlegelii; Vibrio anguillarum; antibacterial properties; bacterial infections; calcium; cysteine; fish; humans; immune response; immunologic receptors; lectins; macrophages; mannose; open reading frames; shellfish; tissues; viruses
Abstract:
... C-type lectin (CTL) is an immune receptor and is received extensive attention of its important roles in immune response and immune escape. Some CTL, such as CTL4, has been well characterized in human and several other mammals, but much less documentation exists about the immunological function of CTL4 in lower vertebrates. In the present study, a C-type lectin domain family 4 member, SsCTL4, which ...
DOI:
10.1016/j.fsi.2018.05.033

84. Complete genome sequence and phylogenetic analysis of megalocytivirus RSIV-Ku: A natural recombination infectious spleen and kidney necrosis virus

Author:
Jing-Yi Shiu; Jiann-Ruey Hong; Chen-Chun Ku; Chiu-Ming Wen
Source:
Archives of virology 2018 v.163 no.4 pp. 1037-1042
ISSN:
0304-8608
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; Pagrus major; adenosinetriphosphatase; coat proteins; genes; genotype; open reading frames; phylogeny; viruses
Abstract:
... Megalocytiviruses are classified into three genotypes, infectious spleen and kidney necrosis virus (ISKNV), red seabream virus (RSIV), and turbo reddish body iridovirus (TRBIV), based on the major capsid protein and ATPase genes. However, only a few complete genome sequences have been obtained. This paper reports the complete genome sequence and phylogenetic analysis of an RSIV-Ku strain megalocyt ...
DOI:
10.1007/s00705-017-3689-2

85. Composition and transcription of all interferon regulatory factors (IRFs), IRF1‒11 in a perciform fish, the mandarin fish, Siniperca chuatsi

Author:
Zubair Ahmed Laghari; Li Li; Shan Nan Chen; Hui Jun Huo; Bei Huang; Ying Zhou; P. Nie
Source:
Developmental and comparative immunology 2018 v.81 pp. 127-140
ISSN:
0145-305X
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; aquaculture; cytokines; fish; gene expression; genes; immune response; immunomodulation; interferon regulatory factors; interferons; kidneys; pathogens; spleen; tissues
Abstract:
... Interferon regulatory factors (IRFs) are a family of mediators in various biological processes including immune modulation of interferon (IFN) and proinflammatory cytokine expression. However, the data on the complete composition of IRFs is rather limited in teleost fish. In the present study, all IRF members, i.e. IRF1‒11 with two IRF4, IRF4a and IRF4b have been characterised in an aquaculture sp ...
DOI:
10.1016/j.dci.2017.11.015

86. First description of a natural infection with spleen and kidney necrosis virus in zebrafish

Author:
Roberto Bermúdez; Ana Paula Losada; Ana Manuela de Azevedo; Jorge Guerra‐Varela; David Pérez‐Fernández; Laura Sánchez; Francesc Padrós; Barbara Nowak; María Isabel Quiroga
Source:
Journal of fish diseases 2018 v.41 no.8 pp. 1283-1294
ISSN:
0140-7775
Subject:
Danio rerio; Infectious spleen and kidney necrosis virus; anorexia; biosecurity; distress; farmed fish; fins; gills; hemorrhage; histopathology; kidneys; models; morbidity; necrosis; pathogenesis; phylogeny; research facilities; spleen; swimming; tissues; viruses; Spain
Abstract:
... Zebrafish has become a popular research model in the last years, and several diseases affecting zebrafish research facilities have been reported. However, only one case of naturally occurring viral infections was described for this species. In 2015, infectious spleen and kidney necrosis virus (ISKNV) was detected in zebrafish from a research facility in Spain. Affected fish showed lethargy, loss o ...
DOI:
10.1111/jfd.12822

87. Functional, signalling and transcriptional differences of three distinct type I IFNs in a perciform fish, the mandarin fish Siniperca chuatsi

Author:
Zubair Ahmed Laghari; Shan Nan Chen; Li Li; Bei Huang; Zhen Gan; Ying Zhou; Hui Jun Huo; Jing Hou; Pin Nie
Source:
Developmental and comparative immunology 2018 v.84 pp. 94-108
ISSN:
0145-305X
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; cytokine receptors; fish; genes; nucleotide sequences; transcription (genetics)
Abstract:
... Teleost fish are unique in having type I and type II interferons (IFNs) only, and the type I IFNs are classified into Group one and Group two based on the presence of two or four cysteines respectively, and are further classified into seven subgroups. In the present study, three distinct type I IFNs, IFNc, IFNd and IFNh, have been identified in the genome sequences of a perciform fish, the mandari ...
DOI:
10.1016/j.dci.2018.02.008

88. Genotype determination of megalocytivirus from Indonesian Marine Fishes

Author:
MURWANTOKO; DINI WAHYU KARTIKA SARI; CHRISTINA RETNO HANDAYANI; RICHARD J. WHITTINGTON
Source:
Biodiversitas 2018 v.19 no.5 pp. 1730-1736
ISSN:
2085-4722
Subject:
Cromileptes altivelis; DNA; DNA-directed DNA polymerase; Epinephelus fuscoguttatus; Gasterosteus aculeatus; Infectious spleen and kidney necrosis virus; Iridovirus; Lates calcarifer; Mycteroperca tigris; Turbot reddish body iridovirus; adenosinetriphosphatase; coat proteins; ethanol; farmed fish; formalin; freshwater; genotype; genotyping; histology; hypertrophy; kidneys; liver; mortality; polymerase chain reaction; spleen; viruses; Indonesia
Abstract:
... Murwantoko, Sari DWK, Handayani CR, Whittington RJ. 2018. Genotype determination of megalocytivirus from Indonesian Marine Fishes. Biodiversitas 19: 1730-1736. Megalocytivirus is the newest genus within the family of Iridoviridae which can be divided into groups represented by red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis virus (ISKNV) and turbot reddish body iridovirus (T ...
DOI:
10.13057/biodiv/d190519

89. Molecular cloning, characterization and expression analysis of complement components in red sea bream (Pagrus major) after Edwardsiella tarda and red sea bream Iridovirus (RSIV) challenge

Author:
Mohammad Ali Noman Reza; Sipra Mohapatra; Sonoko Shimizu; Shin-Ichi Kitamura; Shogo Harakawa; Hidemasa Kawakami; Kei Nakayama; Eitaro Sawayama; Takahiro Matsubara; Kohei Ohta; Tapas Chakraborty
Source:
Fish & shellfish immunology 2018 v.82 pp. 286-295
ISSN:
1050-4648
Subject:
Edwardsiella tarda; Infectious spleen and kidney necrosis virus; Pagrus major; complement; fish; genes; immune response; lectins; liver; messenger RNA; molecular cloning; phylogeny; shellfish; tissue distribution; transcription (genetics)
Abstract:
... The complement system plays an important role in immune regulation and acts as the first line of defense against any pathogenic attack. To comprehend the red sea bream (Pagrus major) immune response, three complement genes, namely, pmC1r, pmMASP and pmC3, belonging to the classical, lectin and alternative complement cascade, respectively, were identified and characterized. pmC1r, pmMASP, and pmC3 ...
DOI:
10.1016/j.fsi.2018.08.027

90. TO17: A teleost antimicrobial peptide that induces degradation of bacterial nucleic acids and inhibits bacterial infection in red drum, Sciaenops ocellatus

Author:
Shu-wen He; Guang-hua Wang; Bin Yue; Shun Zhou; Min Zhang
Source:
Fish & shellfish immunology 2018 v.72 pp. 639-645
ISSN:
1050-4648
Subject:
DNA; Edwardsiella tarda; Infectious spleen and kidney necrosis virus; Micrococcus luteus; RNA; Sciaenops ocellatus; Staphylococcus aureus; Vibrio vulnificus; amino acids; anti-infective agents; antibacterial properties; antimicrobial peptides; aquaculture; bacterial infections; blood cells; blood coagulation; blood serum; cell membranes; cytoplasm; fish; hemolysis; immunostimulants; macrophages; microorganisms; nitric oxide; pathogens; shellfish; tissues
Abstract:
... Tissue factor pathway inhibitor (TFPI)-1 is well known for its role as an inhibitor of blood coagulation. Several studies have demonstrated that the C-terminal peptides of TFPI-1 are active against a broad spectrum of microorganisms. In a previous study, we found that TO17 (with 17 amino acids), a TFPI-1 C-terminal peptide from red drum (Sciaenops ocellatus), was active against Edwardsiella tarda. ...
DOI:
10.1016/j.fsi.2017.11.038

91. iTRAQ-based proteomic profile analysis of ISKNV-infected CPB cells with emphasizing on glucose metabolism, apoptosis and autophagy pathways

Author:
Shiwei Wu; Lujun Yu; Xiaozhe Fu; Xi Yan; Qiang Lin; Lihui Liu; Hongru Liang; Ningqiu Li
Source:
Fish & shellfish immunology 2018 v.79 pp. 102-111
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; Western blotting; apoptosis; autophagy; caspase-8; fish; fumarate hydratase; glucose; glucose 6-phosphate; hosts; industry; pathogenesis; peptides; phosphopyruvate hydratase; protein synthesis; pyruvate dehydrogenase (acetyl-transferring) kinase; pyruvate dehydrogenase (lipoamide); shellfish; tubulin
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) has caused significant losses in the cultured mandarin fish (Siniperca chuatsi) industry. The molecular mechanisms that underlie interaction between ISKNV and hosts are not fully understood. In this study, the proteomic profile of CPB cells at progressive time points after ISKNV infection was analyzed by isobaric tags for relative and absolute qu ...
DOI:
10.1016/j.fsi.2018.05.002

92. The infection of red seabream iridovirus in mandarin fish (Siniperca chuatsi) and the host immune related gene expression profiles

Author:
Xiaodan Liu; Nan Chen; Xiaojian Gao; Yingying Zhang; Xixi Li; Yue Zhang; Xuwen Bing; Hezhong Huang; Xiaojun Zhang
Source:
Fish & shellfish immunology 2018 v.74 pp. 474-484
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Iridovirus; Pagrus major; Siniperca chuatsi; financial economics; fish culture; genes; genotype; hemorrhage; histopathology; immune response; inflammation; interleukin-1beta; interleukin-8; kidneys; leukocytes; liver; marine fish; morbidity; mortality; necrosis; pathogenesis; pathogenicity; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; shellfish; signs and symptoms (animals and humans); spleen; stomach; tissues; transcriptional activation; tumor necrosis factor-alpha
Abstract:
... Red Sea bream iridovirus (RSIV) was initially isolated from marine fish, which belongs to Megalocytivirus, Iridoviridae. It can cause great economic losses in fish culture with high morbidity and mortality. In the present study, the pathogenicity and immune response associated with a RSIV genotype megalocytivirus infection were determined in mandarin fish (Siniperca chuatsi). Fish challenged showe ...
DOI:
10.1016/j.fsi.2018.01.020

93. A natural infection by the red sea bream iridovirus‐type Megalocytivirus in the golden mandarin fish Siniperca scherzeri

Author:
K I Kim; S D Hwang; M Y Cho; S H Jung; Y C Kim; H D Jeong
Source:
Journal of fish diseases 2018 v.41 no.8 pp. 1229-1233
ISSN:
0140-7775
Subject:
Infectious spleen and kidney necrosis virus; Pagrus major; Siniperca scherzeri; Turbot reddish body iridovirus; adenosinetriphosphatase; coat proteins; freshwater fish; genes; genetic variation; phylogeny; quantitative polymerase chain reaction; spleen; Japan; Korean Peninsula
Abstract:
... An outbreak of a Megalocytivirus infection was found in the golden mandarin fish Siniperca scherzeri during September and October 2016, in Korea. Phylogeny and genetic diversity based on the major capsid protein (MCP) and adenosine triphosphatase (ATPase) genes showed a new strain. Designated as GMIV, this strain derived from the golden mandarin fish was suggested to belong to the red sea bream ir ...
DOI:
10.1111/jfd.12815

94. A teleost TFPI-2 peptide that possesses a broad antibacterial spectrum and immune-stimulatory properties

Author:
Shu-wen He; Jing-jing Wang; Xue Du; Bin Yue; Guang-hua Wang; Shun Zhou; Bing Xie; Min Zhang
Source:
Fish & shellfish immunology 2018 v.82 pp. 469-475
ISSN:
1050-4648
Subject:
DNA; Gram-negative bacteria; Gram-positive bacteria; Infectious spleen and kidney necrosis virus; Micrococcus luteus; RNA; Sciaenops ocellatus; Staphylococcus aureus; Vibrio ichthyoenteri; Vibrio scophthalmi; Vibrio vulnificus; amino acids; anti-infective agents; antibacterial properties; antimicrobial peptides; aquaculture; bacterial infections; blood cells; blood coagulation; cell membranes; cytoplasm; fish; hemolysis; humans; immunology; macrophages; pathogens; shellfish; tissues
Abstract:
... Tissue factor pathway inhibitor 2 (TFPI-2) is an analogue of TFPI-1 and a potent endogenous inhibitor of tissue factor (TF)-mediated blood coagulation. Previous reports have shown that several peptides derived from human and vertebrates TFPI-2 possess antibacterial activity against diverse bacteria. In this study, a C-terminal peptide, TO24 (with 24 amino acids), derived from red drum (Sciaenops o ...
DOI:
10.1016/j.fsi.2018.08.051

95. Application and development of a TaqMan real-time PCR for detecting infectious spleen and kidney necrosis virus in Siniperca chuatsi

Author:
Qiang Lin; Xiaozhe Fu; Lihui Liu; Hongru Liang; Huizhi Guo; Shuwen Yin; Venkatesh Kumaresan; Zhibin Huang; Ningqiu Li
Source:
Microbial pathogenesis 2017 v.107 pp. 98-105
ISSN:
0882-4010
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; financial economics; genes; monitoring; quantitative polymerase chain reaction; spleen; tissues
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) is one of the major epidemiological agents that had caused great economic loss in Chinese perch (Siniperca chuatsi). In this study, a specific TaqMan real-time PCR was developed using a pair of primers and a TaqMan probe specific to the ORF007 gene of ISKNV to rapidly detect ISKNV copies in Chinese perch samples. This assay was optimized to produ ...
DOI:
10.1016/j.micpath.2017.02.046

96. Autophagy promoted infectious kidney and spleen necrosis virus replication and decreased infectious virus yields in CPB cell line

Author:
Chen Li; Xiaozhe Fu; Qiang Lin; Lihui Liu; Hongru Liang; Zhibin Huang; Ningqiu Li
Source:
Fish & shellfish immunology 2017 v.60 pp. 25-32
ISSN:
1050-4648
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; autophagy; chloroquine; cytoplasm; industry; pathogenesis; proteins; virus replication; viruses
Abstract:
... Autophagy plays important functions in viral replication and pathogenesis. In this study, we investigated the role of autophagy in the replication of infectious kidney and spleen necrosis virus (ISKNV), an agent that has caused devastating losses in Chinese perch (Siniperca chuatsi) industry. We found that ISKNV infection triggered the complete autophagic process, as demonstrated by microtubule-as ...
DOI:
10.1016/j.fsi.2016.11.037

97. Co-infections of infectious spleen and kidney necrosis virus and Siniperca chuatsi rhabdovirus in Chinese perch (Siniperca chuatsi)

Author:
Qiang Lin; Xiaozhe Fu; Ningqiu Li; Quanyuan Wan; Wenjie Chen; Yunmao Huang; Zhibin Huang; Jun Li; Lijuan Zhao; Li Lin
Source:
Microbial pathogenesis 2017 v.111 pp. 422-430
ISSN:
0882-4010
Subject:
Infectious spleen and kidney necrosis virus; Siniperca chuatsi; brain; farmed fish; mixed infection; pathogens; tissues; vaccines; viral load; viruses
Abstract:
... In spite of the quite common co-infections of viruses in the cultured fish, most of the previous studies have just simply focused on the infection of a single pathogen. In this report, we observed that about 13% of cultured Chinese perch have been co-infected by infectious spleen and kidney necrosis virus (ISKNV) and Siniperca chuatsi rhabdovirus (SCRV). Furthermore, Chinese perch could co-infecte ...
DOI:
10.1016/j.micpath.2017.09.002

98. Development of a highly permissive cell line from spotted knifejaw (Oplegnathus punctatus) for red sea bream iridovirus

Author:
Yasuhiko Kawato; Hirofumi Yamashita; Kei Yuasa; Satoshi Miwa; Kazuhiro Nakajima
Source:
Aquaculture 2017 v.473 pp. 291-298
ISSN:
0044-8486
Subject:
Infectious spleen and kidney necrosis virus; Oplegnathus; cytopathogenicity; mortality; vaccines; viral load
Abstract:
... We developed a cell line (SKF-9) from spotted knifejaw (Oplegnathus punctatus), which is highly susceptible to red sea bream iridovirus (RSIV). Cytopathic effect (CPE) characterized by rounding and enlargement was clearly observed in RSIV-infected SKF-9 cells. The amount of viral production of SKF-9 cells was approximately 100 times greater than that of GF cells, which is conventionally used for R ...
DOI:
10.1016/j.aquaculture.2017.02.027

99. High reduced/oxidized glutathione ratio in infectious spleen and kidney necrosis virus-infected cells contributes to degradation of VP08R multimers

Author:
Jian-Hui He; Muting Yan; Hongliang Zuo; Shengwen Niu; Jia Yuan; Shao-Ping Weng; Jianguo He; Xiaopeng Xu
Source:
Veterinary microbiology 2017 v.207 pp. 19-24
ISSN:
0378-1135
Subject:
Infectious spleen and kidney necrosis virus; NADP (coenzyme); basement membrane; crosslinking; disulfide bonds; endothelial cells; enzymes; fish; glutathione; kidneys; necrosis; spleen; viral proteins; virion; viruses
Abstract:
... Infectious spleen and kidney necrosis virus (ISKNV) is the type species of the genus Megalocytivirus, family Iridoviridae. The ISKNV-infected cells in fish tissues are attached by lymphatic endothelial cells (LECs), which is a unique pathological phenomenon of ISKNV infection. The viral proteins VP23R and VP08R and the host protein nidogen-1 constitute the virus-mock basement membrane (VMBM) on th ...
DOI:
10.1016/j.vetmic.2017.05.024

100. Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major)

Author:
Eitaro Sawayama; Shiho Tanizawa; Shin-Ichi Kitamura; Kei Nakayama; Kohei Ohta; Akiyuki Ozaki; Motohiro Takagi
Source:
Marine biotechnology 2017 v.19 no.6 pp. 601-613
ISSN:
1436-2228
Subject:
Infectious spleen and kidney necrosis virus; Pagrus major; chromosome mapping; disease resistance; females; genes; genetic markers; high-throughput nucleotide sequencing; linkage groups; males; marker-assisted selection; microsatellite repeats; phenotype; phenotypic variation; quantitative trait loci; Japan
Abstract:
... Red sea bream iridoviral disease (RSIVD) is a major viral disease in red sea bream farming in Japan. Previously, we identified one candidate male individual of red sea bream that was significantly associated with convalescent individuals after RSIVD. The purpose of this study is to identify the quantitative trait loci (QTL) linked to the RSIVD-resistant trait for future marker-assisted selection ( ...
DOI:
10.1007/s10126-017-9779-z