U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject term Megasphaera elsdenii Remove constraint Subject term: Megasphaera elsdenii
Start Over

Search

135  Search Results

« Previous | 1 - 100 of 135 |
Number of results to display per page

Search Results

1. Ambient pH regulates lactate catabolism pathway of the ruminal Megasphaera elsdenii BE2‐2083 and Selenomonas ruminantium HD4

Author:
Yaotian Fan; Guangliang Xia; Yaqian Jin; Hongrong Wang
Source:
Journal of applied microbiology 2022 v.132 no.4 pp. 2661-2672
ISSN:
1364-5072
Subject:
Megasphaera elsdenii; Selenomonas ruminantium; catabolism; coculture; enzyme activity; gene expression; lactic acid; mixed culture; nuclear magnetic resonance spectroscopy; pH; ruminal acidosis; sodium
Abstract:
... AIMS: To explore the impact of ambient pH on lactate catabolism by Megasphaera elsdenii BE2‐2083 and Selenomonas ruminantium HD4 in both pure culture and in binary mixed culture. METHODS AND RESULTS: The growth rate, substrate consumption, product formation, enzymatic activity and gene expression of M. elsdenii and S. ruminantium at various pHs were examined. Furthermore, the metabolism of lactate ...
DOI:
10.1111/jam.15464

2. Biohydrogen production by mixed culture of Megasphaera elsdenii with lactic acid bacteria as Lactate-driven dark fermentation

Author:
Akihiro Ohnishi; Yuji Hasegawa; Naoshi Fujimoto; Masaharu Suzuki
Source:
Bioresource technology 2022 v.343 pp. 126076
ISSN:
0960-8524
Subject:
Megasphaera elsdenii; cellobiose; coculture; hydrogen; hydrogen production; lactic acid; lactic fermentation; mixed culture; xylose
Abstract:
... Numerous attempts have been made to upscale biohydrogen production via dark fermentation (DF); however, the Achilles’ heel of DF, i.e., lactic acid bacteria (LAB) contamination and overgrowth, hinders such upscaling. Key microbes are needed to develop a lactate-driven DF system that can serve as a lactate fermentation platform. In this study, the utility of Megasphaera elsdenii and LAB co-culturin ...
DOI:
10.1016/j.biortech.2021.126076

3. Dietary Coleus amboinicus Lour. decreases ruminal methanogenesis and biohydrogenation, and improves meat quality and fatty acid composition in longissimus thoracis muscle of lambs

Author:
Yulianri Rizki Yanza; Malgorzata Szumacher-Strabel; Dorota Lechniak; Sylwester Ślusarczyk; Pawel Kolodziejski; Amlan Kumar Patra; Zora Váradyová; Dariusz Lisiak; Mina Vazirigohar; Adam Cieslak
Source:
Journal of animal science and biotechnology 2022 v.13 no.1 pp. 5
ISSN:
2049-1891
Subject:
Butyrivibrio fibrisolvens; Megasphaera elsdenii; Methanobacteriales; Plectranthus amboinicus; Protozoa; Ruminococcus albus; ammonia; biohydrogenation; biotechnology; digestibility; fatty acid composition; fatty-acid synthase; fermentation; gene expression; lipoprotein lipase; longissimus muscle; meat; meat quality; methane production; methanogens; omega-3 fatty acids; pH; polyphenols; polyunsaturated fatty acids; stearoyl-CoA desaturase
Abstract:
... BACKGROUND: Methane production and fatty acids (FA) biohydrogenation in the rumen are two main constraints in ruminant production causing environmental burden and reducing food product quality. Rumen functions can be modulated by the biologically active compounds (BACs) of plant origins as shown in several studies e.g. reduction in methane emission, modulation of FA composition with positive impac ...
DOI:
10.1186/s40104-021-00654-3

4. Dynamics of microbial communities during inulin fermentation associated with the temporal response in SCFA production

Author:
Siyu Wei; Cheng Wang; Qifan Zhang; Hui Yang; Edward C. Deehan; Xin Zong; Yizhen Wang; Mingliang Jin
Source:
Carbohydrate polymers 2022 v.298 pp. 120057
ISSN:
0144-8617
Subject:
Bifidobacterium thermophilum; Lactobacillus delbrueckii; Megasphaera elsdenii; biochemical pathways; butyrates; dietary fiber; ecological succession; fermentation; intestinal microorganisms; inulin; microbiome; models; regression analysis; swine
Abstract:
... The ecology driving the remodeling of gut microbial consortia with dietary fiber intervention remains incomplete. We investigated the short-term dynamics of the gut microbiota and metabolic function during inulin fermentation with distinct microbiota from two swine breeds using an in vitro fermentation model. Different gut microbiota at a transient temporal time displayed a similar response to inu ...
DOI:
10.1016/j.carbpol.2022.120057

5. Gut microbial profiles and the role in lipid metabolism in Shaziling pigs

Author:
Jie Ma; Yehui Duan; Rui Li; Xiaoxiao Liang; Tiejun Li; Xingguo Huang; Yulong Yin; Jie Yin
Source:
Animal nutrition 2022 v.9 pp. 345-356
ISSN:
2405-6545
Subject:
Clostridium butyricum; Duroc; Lactobacillus amylovorus; Lactobacillus johnsonii; Megasphaera elsdenii; amino acid metabolism; animal models; animal nutrition; bile acids; biosynthesis; cholesterol; digesta; genes; glucose; ileum; intestinal microorganisms; landraces; lipid content; lipid metabolism; liver; low density lipoprotein; metabolites; mucosa; obesity; probiotics; swine
Abstract:
... Shaziling pig, a Chinese indigenous breed, has been classified as a fatty pig model. However, the gut microbial development and role in lipid metabolism in Shaziling pigs has been rarely reported. Here, we compared the lipid metabolic and microbial profiles at 30, 60, 90, 150, 210, and 300 d of age between Shaziling and Yorkshire pigs. Predictably, there were marked differences in the liver lipids ...
DOI:
10.1016/j.aninu.2021.10.012

6. Post-extrusion physical properties, techno-functionality and microbiota-modulating potential of hempseed (Cannabis sativa L.) hull fiber

Author:
William Leonard; Pangzhen Zhang; Danyang Ying; Shuai Nie; Siyao Liu; Zhongxiang Fang
Source:
Food hydrocolloids 2022 v.131 pp. 107836
ISSN:
0268-005X
Subject:
Cannabis sativa; Lactobacillus; Megasphaera elsdenii; X-ray diffraction; byproducts; cannabinoids; crystal structure; extrusion; fermentation; gelatinization; genes; hydrocolloids; inoculum; oils; particle size distribution; phosphopyruvate hydratase; polyphenols; retrogradation; soluble fiber; starch; swine; thermal stability; valeric acid; viscosity; xylose
Abstract:
... One-third of hempseed is composed of fiber, yet this major nutrient is far understudied than the seed's oil, protein, polyphenol or cannabinoid fractions. Here we provided a comprehensive and novel study covering the monosaccharide-linkage composition, physical, functional and microbiota-shifting properties of the fiber-rich (>77%) hempseed hull, which is a major industrial processing by-product. ...
DOI:
10.1016/j.foodhyd.2022.107836

7. Ruminal bacteria lipopolysaccharides: an immunological and microbial outlook

Author:
E. Sarmikasoglou; A. P. Faciola
Source:
Journal of animal science and biotechnology 2022 v.13 no.1 pp. 41
ISSN:
2049-1891
Subject:
Megasphaera elsdenii; O-antigens; animal science; biotechnology; computer simulation; epithelium; glucosamine; immune response; immunomodulators; lipid A; microbiome; oligosaccharides; phosphates; virulence
Abstract:
... Lipopolysaccharides (LPS) are outer membrane components of Gram-negative bacteria made of three regions: the O-antigen; the core oligosaccharide; and a glucosamine disaccharide linked to hydroxy fatty acids, which is named lipid A. The number phosphate groups, and hydroxy fatty acid chains is associated with the immunopotency and the immunomodulatory activity of LPS, where six-acyl chain lipid A w ...
DOI:
10.1186/s40104-022-00692-5

8. The Use of Probiotic Megasphaera elsdenii as a Pre-Harvest Intervention to Reduce Salmonella in Finishing Beef Cattle: An In Vitro Model

Author:
Kellen Habib; James Drouillard; Vanessa de Aguiar Veloso; Grace Huynh; Valentina Trinetta; Sara E. Gragg
Source:
Microorganisms 2022 v.10 no.7 pp. -
ISSN:
2076-2607
Subject:
Megasphaera elsdenii; Salmonella; antibiotics; beef cattle; feces; food chain; models; most probable number technique; pH; probiotics; risk; rumen fluids; ruminal acidosis
Abstract:
... Reducing Salmonella in cattle may mitigate the risk of transmission through the food chain. Megasphaera elsdenii (ME) is a microorganism found naturally in the bovine rumen that can be administered as a probiotic to mitigate ruminal acidosis. Understanding the impact of feeding ME to Salmonella populations in cattle was the objective of this study. Bovine ruminal fluid (RF) and feces were inoculat ...
DOI:
10.3390/microorganisms10071400

9. Effects of dietary oil sources (sunflower and fish) on fermentation characteristics, epithelial gene expression and microbial community in the rumen of lambs fed a high-concentrate diet

Author:
Hamidreza Mirzaei-Alamouti; Arman Abdollahi; Hasan Rahimi; Saeedeh Moradi; Mina Vazirigohar; Jörg R. Aschenbach
Source:
Archives of animal nutrition 2021 v.75 no.6 pp. 405-421
ISSN:
1477-2817
Subject:
Butyrivibrio fibrisolvens; Helianthus annuus; Megasphaera elsdenii; Protozoa; animal nutrition; dietary fat; digesta; dysbiosis; feed concentrates; fermentation; fish; fish oils; gene expression; hydroxymethylglutaryl-CoA synthase; lipopolysaccharides; microbial communities; pH; propionic acid; rumen epithelium; rumen fermentation; slaughter; somatomedins; sterols; sunflower oil
Abstract:
... The feeding of high-concentrate diets commonly results in lowered pH and ruminal dysbiosis which cause shifts in uptake dynamics of short-chain fatty acids (SCFA) and altered epithelial function. Therefore, the current study evaluated the effect of dietary polyunsaturated fatty acids (PUFA) on ruminal fermentation products, gene expression in the ruminal epithelium and the associated changes in ru ...
DOI:
10.1080/1745039X.2021.1997539

10. Effects of feeding urea-treated triticale and oat grain mixtures on ruminal fermentation, microbial population, and milk production performance of midlactation dairy cows

Author:
Kacper Libera; Malgorzata Szumacher-Strabel; Mina Vazirigohar; Wiktor Zieliński; Rafal Lukow; Klaudia Wysocka; Pawel Kołodziejski; Dorota Lechniak; Zora Varadyova; Amlan Kumar Patra; Adam Cieslak
Source:
Annals of animal science 2021 v.21 no.3 pp. 1007-1025
ISSN:
2300-8733
Subject:
Megasphaera elsdenii; Streptococcus bovis; acetates; ammonia; corn; energy; fermentation; grass silage; methanogens; milk yield; nitrogen; oats; pH; profitability; rumen fermentation; rumen fluids; soybean meal; starch; triticale; urea; urease
Abstract:
... The starch content of triticale and oat grains provides much of their readily available energy. Synchronizing energy and nitrogen in the rumen is important in optimizing profitability; for this reason, ammonia processing of these grains was evaluated for its potential to modify ruminal fermentation and to improve milk production performance. A mixture of ground triticale and oats (CONG, at a ratio ...
DOI:
10.2478/aoas-2020-0108

11. Effects of peripartal yeast culture supplementation on lactation performance, blood biomarkers, rumen fermentation, and rumen bacteria species in dairy cows

Author:
N.A. Carpinelli; J. Halfen; E. Trevisi; J.D. Chapman; E.D. Sharman; J.L. Anderson; J.S. Osorio
Source:
Journal of dairy science 2021 v.104 no.10 pp. 10727-10743
ISSN:
0022-0302
Subject:
Fibrobacter succinogenes; Holstein; Megasphaera elsdenii; animal health; biomarkers; blood; corn; crude protein; diet; early lactation; feed intake; fermentation; genetic merit; immune response; milk; milk yield; pregnancy; somatic cells; yeasts
Abstract:
... Feeding yeast culture fermentation products has been associated with improved feed intake and milk yield in transition dairy cows. These improvements in performance have been further described in terms of rumen characteristics, metabolic profile, and immune response. The objective of this study was to evaluate the effects of a commercial yeast culture product (YC; Culture Classic HD, Phibro Animal ...
DOI:
10.3168/jds.2020-20002

12. Effects of ruminally-protected lysine and Megasphaera elsdenii on performance and carcass characteristics of finishing cattle

Author:
Vanessa de Aguiar Veloso; Cadra L. Van Bibber-Krueger; Lucas M. Horton; Kip K. Karges; James S. Drouillard
Source:
Animal feed science and technology 2021 v.275 pp. 114837
ISSN:
0377-8401
Subject:
Megasphaera elsdenii; USDA; alfalfa hay; average daily gain; backfat; beef; body weight; carcass weight; corn; corn gluten; crossbreds; dietary protein; dry matter intake; estradiol; feed conversion; feedlots; feeds; liver abscess; longissimus muscle; lysine; marbling; oral administration; probiotics; protein synthesis; ractopamine; refrigeration; slaughterhouses; trenbolone; tylosin
Abstract:
... Ractopamine hydrochloride is a β-adrenergic agonist (BAA) commonly added to cattle finishing diets that redirects nutrients from fat deposition to protein synthesis and muscle growth. Physiological conditions established by BAA suggests that cattle fed diets containing BAA would have greater requirements for dietary protein to sustain BAA-induced accretion of lean tissue. Three hundred and eighty- ...
DOI:
10.1016/j.anifeedsci.2021.114837

13. Host Factors Affect the Gut Microbiome More Significantly than Diet Shift

Author:
Enkhchimeg Lkhagva; Hea-Jong Chung; Ji-Seon Ahn; Seong-Tshool Hong
Source:
Microorganisms 2021 v.9 no.12 pp. -
ISSN:
2076-2607
Subject:
Bacteroides fragilis; Dialister; Megasphaera elsdenii; Phascolarctobacterium faecium; exercise; intestinal microorganisms; microbiome; vegetarian diet
Abstract:
... The determining factors of the composition of the gut microbiome are one of the main interests in current science. In this work, we compared the effect of diet shift (DS) from heavily relying on meatatarian diets to vegetarian diets and physical exercise (EX) on the composition of the gut microbiome after 3 months. Although both DS and EX affected the composition of the gut microbiome, the pattern ...
DOI:
10.3390/microorganisms9122520

14. Influence of three microbial feed additives of Megasphaera elsdenii, Saccharomyces cerevisiae and Lactobacillus sp. on ruminal methane and carbon dioxide production, and biofermentation kinetics

Author:
E. Direkvandi; T. Mohammadabadi; A.Z.M. Salem
Source:
Journal of applied microbiology 2021 v.131 no.2 pp. 623-633
ISSN:
1364-5072
Subject:
Lactobacillus plantarum; Megasphaera elsdenii; Saccharomyces cerevisiae; acetates; butyrates; carbon dioxide; carbon dioxide production; gas production (biological); greenhouses; lactic acid; methane; pH; propionic acid; risk reduction; ruminal acidosis
Abstract:
... AIMS: This study was performed to investigate the effects of Megasphaera elsdenii (Me), Saccharomyces cerevisiae (SC) and lactic acid bacteria (FP—Lactobacillus fermentum plus Lactobacillus plantarum) alone or in combination on biogas production and ruminal biofermentation parameter in a heterofermenter system. METHODS AND RESULTS: Eight treatments were evaluated; (i) control (without additive; CO ...
DOI:
10.1111/jam.14990

15. Influence of yeast on rumen fermentation, growth performance and quality of products in ruminants: A review

Author:
Abdulmumini B. Amin; Shengyong Mao
Source:
Animal nutrition 2021 v.7 no.1 pp. 31-41
ISSN:
2405-6545
Subject:
Butyrivibrio fibrisolvens; Lactobacillus; Megasphaera elsdenii; Protozoa; Streptococcus bovis; animal nutrition; bacteria; biohydrogenation; butyrates; conjugated linoleic acid; dietary nutrient sources; dosage; environment; fatty acid composition; fatty-acid synthase; genes; growth performance; incidence; lactic acid; linolenic acid; meat; milk; milk quality; muscles; oleic acid; pH; propionic acid; rumen; rumen fermentation; rumen microorganisms; ruminal acidosis; ruminants; saturated fatty acids; stearoyl-CoA desaturase; strains; sugars; transcriptomics; vaccenic acid; very long chain fatty acids; viability; weight gain; yeasts
Abstract:
... This review aims to give an overview of the efficacy of yeast supplementation on growth performance, rumen pH, rumen microbiota, and their relationship to meat and milk quality in ruminants. The practice of feeding high grain diets to ruminants in an effort to increase growth rate and weight gain usually results in excess deposition of saturated fatty acids in animal products and increased inciden ...
DOI:
10.1016/j.aninu.2020.10.005

16. Investigating the Effects of a Phytobiotics-Based Product on the Fecal Bacterial Microbiome of Weaned Pigs

Author:
Anlly Fresno Rueda; Ryan Samuel; Benoit St-Pierre
Source:
Animals 2021 v.11 no.7 pp. -
ISSN:
2076-2615
Subject:
Erysipelotrichaceae; Lactobacillus; Megasphaera elsdenii; Peptostreptococcaceae; Streptococcus; antioxidants; diet; gastrointestinal system; genes; lactic acid; microbiome; physiology; phytonutrients; plant products; propionic acid; psychosocial factors; swine
Abstract:
... The transition to a solid diet, as well as environmental and social stress, have a direct impact on swine gut physiology during weaning, affecting host gastrointestinal functions, as well as resident symbiotic microbial communities. While plant-derived bioactive products, such as phytobiotics, have shown great potential to mitigate these challenges, providing benefits such as antimicrobial, antiox ...
DOI:
10.3390/ani11071950

17. Pulse processing affects gas production by gut bacteria during in vitro fecal fermentation

Author:
Devin J. Rose; Rachana Poudel; Mallory J. Van Haute; Qinnan Yang; Lei Wang; Mukti Singh; Sean Liu
Source:
Food research international 2021 v.147 pp. 110453
ISSN:
0963-9969
Subject:
Bacteroides; Clostridium; Lachnospiraceae; Megasphaera elsdenii; Roseburia; butyrates; carbohydrates; digestive system; fermentation; flatulence; food research; germination; metabolites; microbiome; propionic acid
Abstract:
... Flatulence is one barrier to pulse consumption for many people. Therefore, we examined how processing affects gas production by the microbiome in three classes of pulses. Processing did not affect gas production from Navy beans. However, in Pardina lentils and green peas, (−1.9 ± 0.3 mL/24 h, p < 0.001; −2.3 ± 0.3 mL/24 h, p < 0.001, respectively). In Pardina lentils and green peas, germination di ...
DOI:
10.1016/j.foodres.2021.110453
CHORUS:
10.1016/j.foodres.2021.110453

18. Rumen microbiota and its relation to fermentation in lactose-fed calves

Author:
S. Koike; M. Ueno; H. Miura; A. Saegusa; K. Inouchi; Y. Inabu; T. Sugino; L.L. Guan; M. Oba; Y. Kobayashi
Source:
Journal of dairy science 2021 v.104 no.10 pp. 10744-10752
ISSN:
0022-0302
Subject:
Butyrivibrio; Coriobacteriaceae; Holstein; Megasphaera elsdenii; Mitsuokella; acetates; butyrates; calves; dairy science; digesta; fermentation; genes; growth performance; hay; lactic acid; lactose; propionic acid; quantitative polymerase chain reaction; rumen; rumen microorganisms; starch; succinic acid; texturization
Abstract:
... In our previous studies, we revealed the effect of lactose inclusion in calf starters on the growth performance and gut development of calves. We conducted the present study as a follow-up study to identify the shift in rumen microbiota and its relation to rumen fermentation when calves are fed a lactose-containing starter. Thirty Holstein bull calves were divided into 2 calf starter treatment gro ...
DOI:
10.3168/jds.2021-20225

19. Spectral deconvolution of redox species in the crotonyl-CoA-dependent NADH:ferredoxin oxidoreductase from Megasphaera elsdenii. A flavin-dependent bifurcating enzyme

Author:
Wayne Vigil; Dimitri Niks; Sophie Franz-Badur; Nilanjan Chowdhury; Wolfgang Buckel; Russ Hille
Source:
Archives of biochemistry and biophysics 2021 v.701 pp. 108793
ISSN:
0003-9861
Subject:
Megasphaera elsdenii; biophysics; dithionite; enzymes; flavins; sodium; titration
Abstract:
... We have undertaken a spectral deconvolution of the three FADs of EtfAB/bcd to the spectral changes seen in the course of reduction, including the spectrally distinct anionic and neutral semiquinone states of electron-transferring and bcd flavins. We also demonstrate that, unlike similar systems, no charge-transfer complex is observed on titration of the reduced M. elsdenii EtfAB with NAD⁺. Finally ...
DOI:
10.1016/j.abb.2021.108793

20. The effects of feeding rations that differ in neutral detergent fiber and starch within a day on the daily pattern of key rumen microbial populations

Author:
I.J. Salfer; C.E. Crawford; L.W. Rottman; K.J. Harvatine
Source:
JDS communications 2021 v.2 no.6 pp. 334-339
ISSN:
2666-9102
Subject:
Butyrivibrio fibrisolvens; DNA; Fibrobacter succinogenes; Holstein; Megasphaera elsdenii; Prevotella bryantii; Protozoa; Ruminococcus albus; Selenomonas ruminantium; Streptococcus bovis; cannulas; digesta; high fiber diet; low fiber diet; neutral detergent fiber; quantitative polymerase chain reaction; rumen; starch
Abstract:
... The effect of feeding a single TMR versus multiple rations across the day that differ in concentrations of neutral detergent fiber (NDF) and starch on the daily pattern of rumen microbial populations was characterized. Diets included a control total mixed ration (CON; 33.3% NDF), a low-fiber diet (LF; 29.6% NDF), and a high-fiber diet (HF; 34.8% NDF). Nine cannulated Holstein cows were assigned to ...
DOI:
10.3168/jds.2021-0099

21. Anaerobic production of valeric acid from crude glycerol via chain elongation

Author:
S. T. S. Veras; W. A. Cavalcante; T. A. Gehring; A. R. Ribeiro; T. J. T. Ferreira; M. T. Kato; P. Rojas-Ojeda; J. L. Sanz-Martin; R. C. Leitão
Source:
International journal of environmental science and technology 2020 v.17 no.3 pp. 1847-1858
ISSN:
1735-1472
Subject:
Clostridium; Megasphaera elsdenii; biodiesel; butyrates; chemical oxygen demand; ethanol; fermentation; fuel production; glycerol; hexanoic acid; microbiome; propionic acid; sludge; valeric acid
Abstract:
... Glycerol from biodiesel production was used as a substrate for valerate production in a 13.1-L anaerobic filter with an open microbiome. Ethanol at 15% of the influent chemical oxygen demand was supplemented as an additional electron donor source. The 114-day experimental period was divided in three phases which included initial adaptation phase, external sludge addition from a caproic acid produc ...
DOI:
10.1007/s13762-019-02562-6

22. Biohydrogen production from winery effluents: control of the homoacetogenesis through the headspace gas recirculation

Author:
Germán Buitrón; Karla M Muñoz‐Páez; Guillermo Quijano; Julián Carrillo‐Reyes; Blanca A Albarrán‐Contreras
Source:
Journal of chemical technology and biotechnology 2020 v.95 no.3 pp. 544-552
ISSN:
0268-2575
Subject:
Clostridium ljungdahlii; Megasphaera elsdenii; bacteria; biochemical pathways; biohydrogen; community structure; effluents; gas production (biological); gases; headspace analysis; hydrogen production; liquids; mass transfer; microbial communities; partial pressure; ribosomal DNA; sequence analysis; upflow anaerobic sludge blanket reactor
Abstract:
... BACKGROUND: Fermentative hydrogen production has an inherent limitation caused by hydrogen‐consuming metabolic pathways such as homoacetogenesis related to high hydrogen partial pressures. In this study, a strategy based on recirculating the headspace gas was applied to increase the hydrogen release from the liquid to the gas phase in an upflow anaerobic sludge blanket(UASB) reactor fed with winer ...
DOI:
10.1002/jctb.6263

23. Effects of repeated oral inoculation of artificially fed lambs with lyophilized rumen fluid on growth performance, rumen fermentation, microbial population and organ development

Author:
Shaobo Yu; Weibing Shi; Bin Yang; Ge Gao; Hongwei Chen; Lei Cao; Zhongtang Yu; Jiakun Wang
Source:
Animal feed science and technology 2020 v.264 pp. 114465
ISSN:
0377-8401
Subject:
Megasphaera elsdenii; Protozoa; Ruminococcus flavefaciens; Selenomonas ruminantium; amylases; bacteria; beta-glucosidase; carbohydrates; crude protein; digestibility; digestible carbohydrates; digestible protein; energy; enzyme activity; feeds; freeze drying; fungi; growth performance; lambs; liver; oral administration; population dynamics; propionic acid; rumen bacteria; rumen fermentation; rumen fluids; temporal variation; weaning; xylanases
Abstract:
... Early life development and growth are critical in determining the lifelong growth performance and health of ruminants, and weaning poses the greatest challenge to young animals and has the most profound consequence. In this study, we aimed to investigate the effects of microbial intervention on the growth and development of young lambs and their rumen microbiota as they went through weaning. Forty ...
DOI:
10.1016/j.anifeedsci.2020.114465

24. Effects of replacing starch with three sugars in a concentrate and forage diet on in vitro rumen fermentation, fatty acid composition and related bacteria

Author:
X. Q. Sun; Y. P. Wang; R. Y. Wei; B. Chen; X. Zhao
Source:
Animal production science 2020 v.60 no.9 pp. 1173-1182
ISSN:
1836-0939
Subject:
Megasphaera elsdenii; acetates; ammonium nitrogen; bacteria; biohydrogenation; butyrates; corn starch; diet; energy density; experimental design; fatty acid composition; feed concentrates; fermentation; forage; fructose; isomers; lactose; milk fat; milk fat yield; pH; propionic acid; risk reduction; rumen; rumen bacteria; rumen fermentation; ruminal acidosis; starch; sucrose; sugar feeding; volatile fatty acids
Abstract:
... Context Replacing starch with sugar could maintain dietary energy density with reduced risks of rumen acidosis and milk fat depression, but the underlying mechanism is not well understood, and the effects of sugar feeding might vary among sugars. Aims Objectives of the present study were to evaluate the effects of replacing corn starch in a diet containing 40:60 forage-to-concentrate ratio (contro ...
DOI:
10.1071/AN18287

25. Genome-Scale Metabolic Network Reconstruction and In Silico Analysis of Hexanoic acid Producing Megasphaera elsdenii

Author:
Na-Rae Lee; Choong Hwan Lee; Dong-Yup Lee; Jin-Byung Park
Source:
Microorganisms 2020 v.8 no.4 pp. -
ISSN:
2076-2607
Subject:
Megasphaera elsdenii; acetyl coenzyme A; biomass; carboxy-lyases; cell growth; computer simulation; hexanoic acid; metabolism; metabolites; models; succinic acid; tricarboxylic acid cycle; value added
Abstract:
... Hexanoic acid and its derivatives have been recently recognized as value-added materials and can be synthesized by several microbes. Of them, Megasphaera elsdenii has been considered as an interesting hexanoic acid producer because of its capability to utilize a variety of carbons sources. However, the cellular metabolism and physiology of M. elsdenii still remain uncharacterized. Therefore, in or ...
DOI:
10.3390/microorganisms8040539

26. Impact of feedstocks and downstream processing technologies on the economics of caproic acid production in fermentation by Megasphaera elsdenii T81

Author:
Hyunjin Kim; Okkyoung Choi; Byoung Seung Jeon; Woo-Seok Choe; Byoung-In Sang
Source:
Bioresource technology 2020 v.301 pp. 122794
ISSN:
0960-8524
Subject:
Jerusalem artichokes; Megasphaera elsdenii; carbon; distillation; feedstocks; fermentation; hexanoic acid; hydrolysates; market prices; oleyl alcohols; purchasing; solvents; sucrose; tubers
Abstract:
... Caproic acid (CA) was produced by Megasphaera elsdenii T81 with Jerusalem artichoke tubers (JA) as a feedstock. More CA was produced under the medium with the acid hydrolysate of JA than the comparative medium with a carbon composition similar to that of JA. CA was produced up to 13.0 g/L and 0.52 g/L/h with extractive fermentation using a mixed solvent of alamine 336 in oleyl alcohol at 37 °C. Th ...
DOI:
10.1016/j.biortech.2020.122794

27. Oral administration of lactate producing bacteria alone or combined with Saccharomyces cerevisiae and Megasphaera elsdenii on performance of fattening lambs

Author:
Ehsan Direkvandi; Tahereh Mohammadabadi; Abdelfattah Z. M. Salem
Source:
Journal of applied animal research 2020 v.48 no.1 pp. 235-243
ISSN:
0974-1844
Subject:
Lactobacillus fermentum; Lactobacillus plantarum; Megasphaera elsdenii; Saccharomyces cerevisiae; acetates; ammonium nitrogen; animal research; average daily gain; blood; butyrates; digestibility; feed conversion; feed intake; growth performance; lactic acid; oral administration; organic matter; pH; propionic acid; rumen fermentation
Abstract:
... To investigate the effect of direct fed microbial (DFM) on performance of fattening lamb, 4 treatments (7 animals/treatment) were evaluated; (1) control (without additive; CON); (2) Lactobacillus fermentum and Lactobacillus plantarum (FP); (3) Saccharomyces cerevisiae (SC) plus FP (SCFP) and (4) Megasphaera elsdenii plus SCFP (MSCFP). Feed intake, final body and feed efficiency except average dail ...
DOI:
10.1080/09712119.2020.1773830

28. True and Illusory Benefits of Modeling: Comment on “Genome-Scale Metabolic Network Reconstruction and In Silico Analysis of Hexanoic Acid Producing Megasphaera elsdenii. Microorganisms 2020, 8, 539”

Author:
Nicolai S. Panikov
Source:
Microorganisms 2020 v.8 no.11 pp. -
ISSN:
2076-2607
Subject:
Megasphaera elsdenii; biochemical pathways; bioprocessing; biosynthesis; computer simulation; hexanoic acid; models
Abstract:
... Lee et al. [1] recently published a paper that is part of the special issue “Genome-Scale Modeling of Microorganisms in the Real World”. In this article, the authors selected Megasphaera elsdenii as a promising industrial producer of hexanoic acid and developed the genome-scale model that has been claimed to improve the mechanistic understanding of the metabolic control of microbial biosynthesis i ...
DOI:
10.3390/microorganisms8111742

29. The bio‐surfactant mannosylerythritol lipid acts as a selective antibacterial agent to modulate rumen fermentation

Author:
Kana Okuhira; Satoshi Koike; Shinji Ito; Yasuo Kobayashi
Source:
Animal science journal = 2020 v.91 no.1 pp. e13464
ISSN:
1344-3941
Subject:
Fibrobacter succinogenes; Megasphaera elsdenii; Pseudozyma; Selenomonas ruminantium; Streptococcus bovis; Succinivibrio dextrinosolvens; acetates; animal science; antibiotics; biosurfactants; butyrates; diet; dose response; esterification; fermentation; hydrogen; lipids; methane production; nutrient utilization; propionic acid; sheep; sugars
Abstract:
... Methyl‐mannosylerythritol lipid (MEL), a new sugar esterified lipid synthesized by Pseudozyma aphidis, was assessed for its functionality in modulating rumen fermentation and microbiota toward more propionate and less methane production. A pure culture study using rumen representatives showed that MEL selectively inhibited the growth of most Gram‐positive bacteria including Streptococcus bovis, ru ...
DOI:
10.1111/asj.13464

30. Assessment of probiotic effects of isolated Megasphaera elsdenii strains in Mehraban sheep and Holstein lactating cows

Author:
Reza Sedighi; Daryoush Alipour
Source:
Animal feed science and technology 2019 v.248 pp. 126-131
ISSN:
0377-8401
Subject:
Holstein; Megasphaera elsdenii; bacteria; carbon dioxide; dairy cows; diet; forage; in vitro studies; lactate dehydrogenase; lactating females; lactation; lactic acid; milk fat; mixed culture; pH; probiotics; rumen; rumen microorganisms; ruminal acidosis; sheep; volatile fatty acids
Abstract:
... This study was aimed to evaluate the potential of lactic acid utilizing bacteria in controlling ruminal acidosis in vitro in sheep and their effect as direct fed microbial in dairy cow nutrition. In first in vitro trial, effects of four isolates of Megasphaera elsdenii (SA1, SA2, SA3 and SA4) that were isolated from rumen of Holstein cows were examined on decrease of lactic acid and control of pH ...
DOI:
10.1016/j.anifeedsci.2019.01.007

31. Effect of sugarcane genotype and maturity stage at harvest on feed intake and ruminal parameters of growing steers

Author:
D.O. Sousa; A.V. Velasquez; C.A. Oliveira; J.M. Souza; E. Nadeau; L.F.P. Silva
Source:
Animal feed science and technology 2019 pp. -
ISSN:
0377-8401
Subject:
Fibrobacter succinogenes; Megasphaera elsdenii; Nellore; Ruminococcus albus; biochemical pathways; cannulas; digestibility; early development; experimental diets; feed intake; genotype; maturity stage; pH; propionic acid; rumen; rumen bacteria; rumen fermentation; satiety; short chain fatty acids; silage; steers; sugarcane
Abstract:
... This experiment was conducted to evaluate the effect of altering fibre digestibility by genotype and maturity stage of sugarcane silage on intake, ruminal fermentation, ruminal kinetics, and rumen bacteria populations. Two genotypes with high or low NDF digestibility (NDFD) harvested for silage at early or late maturity were used in a 2 × 2 factorial arrangement of treatments: two genotypes and tw ...
DOI:
10.1016/j.anifeedsci.2019.114258

32. Effects of certain long-chain fatty acid combinations on the ruminal microbe species relating to fermentation type in vitro

Author:
Y.J. Jing; Y.F. Wang; M.Z. Wang; J. Gao; J.L. Ouyang; L. Cheng
Source:
Indian journal of animal research 2019 v.53 no.6 pp. 768-773
ISSN:
0976-0555
Subject:
Butyrivibrio fibrisolvens; Clostridium proteoclasticum; Fibrobacter succinogenes; Megasphaera elsdenii; Pseudobutyrivibrio ruminis; Ruminobacter amylophilus; Ruminococcus albus; Ruminococcus bromii; animal research; butyric acid; cannulas; culture media; dominant species; fermentation; flora; linoleic acid; linolenic acid; microbial communities; oleic acid; palmitic acid; propionic acid; rumen microorganisms; stearic acid
Abstract:
... This experiment was conducted to investigate the effects of certain long-chain fatty acid combinations on ruminal microbial community in vitro. Three cows fitted with permanent ruminal cannulas were used to provide rumen liquor for the in vitro trail. The treatments were fatty acid combinations of stearic acid, oleic acid, linoleic acid and linolenic acid, which respectively were group A (calcium ...
DOI:
10.18805/ijar.B-802

33. Effects of commercial aldehydes from green leaf volatiles (green odour) on rumen microbial population and fermentation profile in an artificial rumen (Rusitec)

Author:
Ahu Demirtas; Hakan Ozturk; Mert Sudagidan; Erhan Keyvan; Orhan Yavuz; Ozlem Yildiz Gulay; Saad Ahmed Adam Musa
Source:
Anaerobe 2019 v.55 pp. 83-92
ISSN:
1075-9964
Subject:
Butyrivibrio fibrisolvens; Fibrobacter succinogenes; Megasphaera elsdenii; Protozoa; Ruminococcus albus; Ruminococcus flavefaciens; Selenomonas ruminantium; acetates; aldehydes; ammonia; ammonium nitrogen; bacteria; butyrates; dry matter digestibility; energy efficiency; fermentation; metabolites; methane production; methanogens; minimum inhibitory concentration; monensin; odors; propionic acid; rumen; rumen bacteria; rumen fermentation; rumen protozoa; volatile fatty acids
Abstract:
... The effects of plant metabolites on rumen metabolism vary greatly depending on their antimicrobial spectrum and applied doses. In this study, the minimum inhibitory concentrations (MICs) of commercial aldehydes, trans-2-hexenal (T2H), cis-3-hexenal (C3H), trans-2-nonenal (T2N), and trans-2-decenal (T2D) from green leaf volatiles, were tested on rumen bacteria. These compounds were found more effec ...
DOI:
10.1016/j.anaerobe.2018.11.001

34. Effects of energy sources and inclusion levels of concentrate in sugarcane-silage-based diets of finishing Nellore young bulls: Nutrient digestibility, rumen metabolism and ecosystem

Author:
V. B. Ferrari; N. R. B. Cônsolo; R. T. Sousa; J. M. Souza; I. C. S. Bueno; L. F. P. Silva
Source:
Journal of agricultural science 2019 v.157 no.4 pp. 357-364
ISSN:
1469-5146
Subject:
Fibrobacter succinogenes; Megasphaera elsdenii; Nellore; Ruminococcus albus; Ruminococcus flavefaciens; Streptococcus bovis; acetic acid; agricultural sciences; ammonia; citrus pulp; corn; diet; digestibility; digestible carbohydrates; digestible organic matter; ecosystems; energy; feed concentrates; fermentation; neutral detergent fiber; organic matter; pH; propionic acid; starch; steers
Abstract:
... Intake in sugar-rich diets can be limited either via rumen fill or excessive rumen fermentation and source of non-fibre carbohydrate (NFC) in the diet can affect both factors. The aim of the current study was to quantify the effect of partially replacing ground maize (GM) with steam-rolled maize (SRM) or pelleted citrus pulp (PCP) at two concentrate levels in sugarcane-based diets on digestibility ...
DOI:
10.1017/S0021859619000534

35. Effects of live yeast on differential genetic and functional attributes of rumen microbiota in beef cattle

Author:
Ibukun M. Ogunade; Jerusha Lay; Kenneth Andries; Christina J. McManus; Frederick Bebe
Source:
Journal of animal science and biotechnology 2019 v.10 no.1 pp. 68
ISSN:
2049-1891
Subject:
Desulfovibrio desulfuricans; Desulfovibrio vulgaris; Holstein; Megasphaera elsdenii; Ruminococcus albus; Ruminococcus bromii; Ruminococcus obeum; Trifolium pratense; beef cattle; beta-alanine; biosynthesis; carbohydrate binding; cellulolytic bacteria; coenzyme A; cross-over studies; databases; diet; genes; glutathione; glycosidases; glycosyltransferases; hay; lipopolysaccharides; metagenomics; orchards; oxidative phosphorylation; polyketides; protein transport; ribosomes; rumen fermentation; rumen fluids; rumen microorganisms; steers; sugars; yeasts
Abstract:
... Several studies have evaluated the effects of live yeast supplementation on rumen microbial population; however, its effect on differential microbial genes and their functional potential has not been described. Thus, this study applied shotgun metagenomic sequencing to evaluate the effects of live yeast supplementation on genetic and functional potential of the rumen microbiota in beef cattle. Eig ...
DOI:
10.1186/s40104-019-0378-x
PubMed:
31508230
PubMed Central:
PMC6724239
CHORUS:
10.1186/s40104-019-0378-x

36. Efficacy of Megasphaera elsdenii inoculation in subacute ruminal acidosis in cattle

Author:
Huzur Derya Arik; Nurettin Gulsen; Armagan Hayirli; Mustafa Selcuk Alatas
Source:
Journal of animal physiology and animal nutrition 2019 v.103 no.2 pp. 416-426
ISSN:
0931-2439
Subject:
Megasphaera elsdenii; Protozoa; ammonia; cannulas; corn; diet; heifers; lactic acid; microprocessors; pH; propionic acid; rumen fluids; rumen microorganisms; ruminal acidosis; volatile fatty acids; wheat
Abstract:
... Two consecutive experiments were carried out to determine efficacy of Megasphaera elsdenii inoculation in alleviation of subacute ruminal acidosis (SARA). In the first experiment, SARA was induced by feeding corn‐ and wheat‐based diets (20%, 40%, 60% and 80% of TMR, DM basis) in six ruminally cannulated heifers. Continuous pH was obtained using data loggers embedded in rumen. In corn (80%)‐ and wh ...
DOI:
10.1111/jpn.13034

37. Megasphaera elsdenii Attenuates Lactate Accumulation in Cultures of Equine Cecal Microorganisms Provided With Starch or Oligofructose

Author:
Teresa L. Douthit; Hannah R. Leventhal; Solange Uwituze; Michael Y. Halpin; Ana Laura Araújo Lopes; James S. Drouillard
Source:
Journal of equine veterinary science 2019 v.74 pp. 1-8
ISSN:
0737-0806
Subject:
Megasphaera elsdenii; butyrates; cannulas; corn starch; fructooligosaccharides; horses; inoculum; intestinal microorganisms; inulin; lactic acid; pH; propionic acid; starch
Abstract:
... The effects of adding Megasphaera elsdenii (ME) to equine cecal microorganisms provided oligofructose (inulin [IN]) or corn starch (CS) were evaluated. Four cecally cannulated horses (529 ± 70 kg BW) were used as donors of cecal inoculum for randomized complete block experiments with 2 × 2 factorial treatment arrangements. Factor 1 was carbohydrate source (IN or CS). Factor 2 was level of added ME ...
DOI:
10.1016/j.jevs.2018.12.013

38. Screening, expression, purification and characterization of CoA-transferases for lactoyl-CoA generation

Author:
Xiaoxia Zhang; Yufeng Mao; Baowei Wang; Zhenzhen Cui; Zhidan Zhang; Zhiwen Wang; Tao Chen
Source:
Journal of industrial microbiology & biotechnology 2019 v.46 no.7 pp. 899-909
ISSN:
1367-5435
Subject:
Clostridium propionicum; CoA-transferases; Megasphaera elsdenii; acetyl coenzyme A; amino acid sequences; bacteria; biodegradability; biosynthesis; catalytic activity; composite polymers; engineering; lactic acid; screening; sequence homology; temperature
Abstract:
... Lactoyl-CoA is critical for the biosynthesis of biodegradable and biocompatible lactate-based copolymers, which have wide applications. However, reports on acetyl-CoA: lactate CoA-transferases (ALCTs) are rare. To exploit novel ALCTs, amino acid sequence similarity searches based on the CoA-transferases from Clostridium propionicum and Megasphaera elsdenii were conducted. Two known and three novel ...
DOI:
10.1007/s10295-019-02174-6

39. Changes in ruminal fermentation and microbial population of feedlot Nellore cattle fed crude glycerin and virginiamycin

Author:
Pablo S. Castagnino; Erick E. Dallantonia; Giovani Fiorentini; Elias San Vito; Juliana D. Messana; Laís O. Lima; Tiago A. Simioni; Telma T. Berchielli
Source:
Animal feed science and technology 2018 v.242 pp. 69-76
ISSN:
0377-8401
Subject:
Megasphaera elsdenii; Nellore; Protozoa; bacteria; bacterial growth; bulls; butyrates; corn; data analysis; diet; feedlots; fermented foods; glycerol; lactic acid; nutrients; pH; propionic acid; roughage; rumen; rumen fermentation; sugarcane bagasse; virginiamycin
Abstract:
... The objective of this experiment was to evaluate the effects of crude glycerin (CG) and virginiamycin (VM) diets on ruminal fermentation and microbial population of feedlot Nellore cattle. Eight rumen fistulated bulls (BW = 600 ± 34 kg; 26 ± 3 months) were used in a replicated 4 × 4 Latin square (21-d periods) with 2 × 2 factorial arrangement of treatments: diets without virginiamycin (VM−) or vir ...
DOI:
10.1016/j.anifeedsci.2018.05.007

40. Dairy propionibacteria as direct-fed microbials: in vitro effect on acid metabolism of Streptococcus bovis and Megasphaera elsdenii

Author:
Jianbiao Luo; Chaminda Senaka Ranadheera; Stuart King; Craig Andrew Evans; Surinder Baines
Source:
Annals of microbiology 2018 v.68 no.3 pp. 153-158
ISSN:
1590-4261
Subject:
Megasphaera elsdenii; Streptococcus bovis; beef cattle; coculture; dairy cows; digesta; glucose; lactic acid; metabolism; pH; rumen; rumen fermentation; ruminal acidosis
Abstract:
... Ruminal acidosis caused by accumulation of lactic acid, a decrease of pH in the rumen and subsequent imbalance of the rumen fermentation process, affects the health and productivity of dairy cows and beef cattle. Direct-fed microbials have potential for use in the control and prevention of ruminal acidosis. This study investigated the interaction between five strains of dairy propionibacteria, Meg ...
DOI:
10.1007/s13213-018-1322-6

41. Effects of dietary protein levels and calcium salts of long-chain fatty acids on nitrogen mobilization, rumen microbiota and plasma fatty acid composition in Holstein bulls

Author:
Yang He; Zhantao Yu; Qinghua Qiu; Taoqi Shao; Wenjing Niu; Chuanqi Xia; Haibo Wang; Huawei Su; Binghai Cao
Source:
Animal feed science and technology 2018 v.246 pp. 1-10
ISSN:
0377-8401
Subject:
Butyrivibrio fibrisolvens; Holstein; Megasphaera elsdenii; ammonium nitrogen; bulls; calcium; crude protein; dietary protein; dietary supplements; digestibility; excretion; fatty acid composition; finishing; greenhouse gas emissions; long chain fatty acids; low density lipoprotein cholesterol; methane; methane production; methanogens; nitrogen; nutrient intake; organic matter; pollution; rumen; rumen fermentation; rumen microorganisms; salts; stearoyl-CoA desaturase; urea; wastes
Abstract:
... The aim of this study was to investigate the impact of dietary crude protein (CP) levels and calcium salts of long-chain fatty acids (CSFA) on the nutrient intake and digestibility, nitrogen deposition, rumen fermentation characteristic and microbiota, plasma biochemical indexes and fatty acid composition in the Holstein bulls. Eight Holstein bulls was used in a 4 × 4 Latin Square design with 2 ×  ...
DOI:
10.1016/j.anifeedsci.2018.09.019

42. Effects of live yeast (Saccharomyces cerevisiae) and type of cereal on rumen microbial fermentation in a dual flow continuous culture fermentation system

Author:
Diego Moya; Alfred Ferret; Marta Blanch; Mari Carmen Fuentes; Jose Ignacio Fandiño; Sergio Calsamiglia
Source:
Journal of animal physiology and animal nutrition 2018 v.102 no.6 pp. 1488-1496
ISSN:
0931-2439
Subject:
Megasphaera elsdenii; Saccharomyces cerevisiae; Streptococcus bovis; additives; ammonium nitrogen; barley; corn; crude protein; diet; digestibility; fermentation; fermenters; forage; pH; peptides; propionic acid; protein degradation; rumen fermentation; rumen microorganisms; starch; yeasts
Abstract:
... Live yeast additives may help optimize ruminal fermentation of high‐grain diets, especially when the starch of the ration is highly fermentable. Eight dual flow continuous culture fermenters were used in a 2 × 2 factorial design in two replicated periods of 9 days (six for adaptation and three for sampling) to determine the effect of live yeast and type of cereal on rumen microbial fermentation an ...
DOI:
10.1111/jpn.12975

43. Engineering an [FeFe]-Hydrogenase: Do Accessory Clusters Influence O2 Resistance and Catalytic Bias?

Author:
Giorgio Caserta; Cecilia Papini; Agnieszka Adamska-Venkatesh; Ludovic Pecqueur; Constanze Sommer; Edward Reijerse; Wolfgang Lubitz; Charles Gauquelin; Isabelle Meynial-Salles; Debajyoti Pramanik; Vincent Artero; Mohamed Atta; Melisa del Barrio; Bruno Faivre; Vincent Fourmond; Christophe Léger; Marc Fontecave
Source:
Journal of the American Chemical Society 2018 v.140 no.16 pp. 5516-5526
ISSN:
1520-5126
Subject:
Megasphaera elsdenii; active sites; algae; biocatalysts; catalytic activity; enzyme activity; hydrogen; oxygen; spectroscopy
Abstract:
... [FeFe]-hydrogenases, HydAs, are unique biocatalysts for proton reduction to H₂. However, they suffer from a number of drawbacks for biotechnological applications: size, number and diversity of metal cofactors, oxygen sensitivity. Here we show that HydA from Megasphaera elsdenii (MeHydA) displays significant resistance to O₂. Furthermore, we produced a shorter version of this enzyme (MeH-HydA), lac ...
DOI:
10.1021/jacs.8b01689

44. Lifespan Extension of Caenorhabditis elegans by Butyricicoccus pullicaecorum and Megasphaera elsdenii with Probiotic Potential

Author:
Gayeung Kwon; Jiyun Lee; Jong-Ho Koh; Young-Hee Lim
Source:
Current microbiology 2018 v.75 no.5 pp. 557-564
ISSN:
0343-8651
Subject:
Caenorhabditis elegans; Escherichia coli; Megasphaera elsdenii; Salmonella Typhimurium; animal pathogens; biomarkers; body size; humans; innate immunity; intestines; longevity; loss-of-function mutation; mutants; probiotics; transforming growth factor beta
Abstract:
... Butyricicoccus pullicaecorum and Megasphaera elsdenii inhabit the human intestine and have probiotic potential. The aim of this study was to evaluate the effects of B. pullicaecorum and M. elsdenii on the lifespan of Caenorhabditis elegans. They significantly (P < 0.05) extended the lifespan of C. elegans compared with Escherichia coli OP50, a standard food for the worm. Analysis of age-related bi ...
DOI:
10.1007/s00284-017-1416-6

45. Oral administration of Megasphaera elsdenii to Jersey cows during early lactation

Author:
D. Ye; M.L. Eastridge
Source:
Professional animal scientists 2018 v.34 no.1 pp. 67-74
ISSN:
1080-7446
Subject:
Jersey; Megasphaera elsdenii; blood serum; calving; cows; diet; dry matter intake; early lactation; feed conversion; forage; free stalls; ketones; metabolic diseases; milk; milk fat; milk yield; oral administration; risk reduction; starch; tie stalls; urine; Colorado
Abstract:
... The objective of this study was to determine whether Megasphaera elsdenii orally administered to transition Jersey cows would improve milk yield and reduce the risk for metabolic disease. Thirty primi- and multiparous Jersey cows, blocked according to parity and date of calving, were used in a randomized complete block design until 90 DIM and fed diets with 51% forage and 17.9 to 28.5% starch. Wit ...
DOI:
10.15232/pas.2017-01668

46. Reproducible, high-yielding, biological caproate production from food waste using a single-phase anaerobic reactor system

Author:
Corine Orline Nzeteu; Anna Christine Trego; Florence Abram; Vincent O’Flaherty
Source:
Biotechnology for biofuels 2018 v.11 no.1 pp. 108
ISSN:
1754-6834
Subject:
Clostridium kluyveri; Megasphaera elsdenii; Ruminococcaceae; anaerobic digesters; anaerobic digestion; bacteria; biogas; carbon; chemical oxygen demand; feedstocks; fermentation; food waste; greenhouse gas emissions; hexanoic acid; hydrogen; hydrolysis; lactic acid; leachates; microbial communities; mixed culture; production costs
Abstract:
... BACKGROUND: Nowadays, the vast majority of chemicals are either synthesised from fossil fuels or are extracted from agricultural commodities. However, these production approaches are not environmentally and economically sustainable, as they result in the emission of greenhouse gases and they may also compete with food production. Because of the global agreement to reduce greenhouse gas emissions, ...
DOI:
10.1186/s13068-018-1101-4
PubMed:
29651303
PubMed Central:
PMC5894149

47. Residual feed intake in beef cattle and its association with carcass traits, ruminal solid-fraction bacteria, and epithelium gene expression

Author:
Ahmed A. Elolimy; Mohamed K. Abdelmegeid; Joshua C. McCann; Daniel W. Shike; Juan J. Loor
Source:
Journal of animal science and biotechnology 2018 v.9 no.1 pp. 67
ISSN:
2049-1891
Subject:
Eubacterium ruminantium; Fibrobacter succinogenes; Megasphaera elsdenii; Red Angus; Succinimonas amylolytica; absorption; average daily gain; bacteria; beef cattle; biomarkers; body weight; carcass weight; diet; dry matter intake; feed conversion; finishing; gene expression; genes; growth performance; heart; heifers; herds; kidneys; meat cuts; messenger RNA; metabolism; phenotype; quantitative polymerase chain reaction; rumen bacteria; rumen epithelium; slaughter; steers; transcription (genetics)
Abstract:
... BACKGROUND: Residual feed intake (RFI) describes an animal’s feed efficiency independent of growth performance. The objective of this study was to determine differences in growth performance, carcass traits, major bacteria attached to ruminal solids-fraction, and ruminal epithelium gene expression between the most-efficient and the least-efficient beef cattle. One-hundred and forty-nine Red Angus ...
DOI:
10.1186/s40104-018-0283-8
PubMed:
30258628
PubMed Central:
PMC6151901

48. Rumen-protected methionine during the peripartal period in dairy cows and its effects on abundance of major species of ruminal bacteria

Author:
Mohamed K. Abdelmegeid; Ahmed A. Elolimy; Zheng Zhou; Vincenzo Lopreiato; Joshua C. McCann; Juan J. Loor
Source:
Journal of animal science and biotechnology 2018 v.9 no.1 pp. 17
ISSN:
2049-1891
Subject:
Anaerovibrio lipolyticus; Butyrivibrio; Eubacterium ruminantium; Fibrobacter succinogenes; Megasphaera elsdenii; Prevotella bryantii; Selenomonas ruminantium; Streptococcus bovis; Succinimonas amylolytica; Succinivibrio dextrinosolvens; biohydrogenation; cellulose; corn; dairy cows; deamination; diet; digestion; dry matter intake; lactic acid; lipids; lysine; methionine; microbiome; milk production; parturition; prepartum period; propionic acid; proteolysis; rumen; rumen bacteria; xylan
Abstract:
... BACKGROUND: Extensive degradation of amino acids in the rumen via microbial deamination decreases the post-ruminal availability of dietary indispensable amino acids. Together with the normal decrease in voluntary dry matter intake (DMI) around parturition in dairy cows, microbial metabolism contributes to a markedly negative balance of indispensable amino acids, including methionine which may be t ...
DOI:
10.1186/s40104-018-0230-8
PubMed:
29445454
PubMed Central:
PMC5801671

49. Short communication: Does early-life administration of a Megasphaera elsdenii probiotic affect long-term establishment of the organism in the rumen and alter rumen metabolism in the dairy calf?

Author:
T.T. Yohe; B.D. Enger; L. Wang; H.L.M. Tucker; C.A. Ceh; C.L.M. Parsons; Z. Yu; K.M. Daniels
Source:
Journal of dairy science 2018 v.101 no.2 pp. 1747-1751
ISSN:
0022-0302
Subject:
3-hydroxybutyric acid; Holstein; Megasphaera elsdenii; autoclaving; blood; blood glucose; blood sampling; body weight; butyrates; dairy calves; heifers; lactic acid; metabolites; oral administration; probiotics; rumen; rumen development; rumen fermentation; volatile fatty acids
Abstract:
... Megasphaera elsdenii is a bacterial species of the rumen that can utilize lactate to produce butyrate, a key volatile fatty acid often implicated in driving calf rumen development. Because lactate is abundant in the rumen of young calves, administration of M. elsdenii to increase butyrate production and thus promote calf rumen development is an appealing possibility. The main objective of this stu ...
DOI:
10.3168/jds.2017-12551

50. Valerate production by Megasphaera elsdenii isolated from pig feces

Author:
Shota Yoshikawa; Ryosuke Araoka; Yu Kajihara; Toshiyuki Ito; Hirokuni Miyamoto; Hiroaki Kodama
Source:
Journal of bioscience and bioengineering 2018 v.125 no.5 pp. 519-524
ISSN:
1389-1723
Subject:
Megasphaera elsdenii; acetates; butyrates; digestive system; energy; farms; feces; fermentation; genotyping; lactic acid; propionic acid; swine; Japan
Abstract:
... Megasphaera elsdenii is able to produce several short-chain fatty acids (SCFAs), such as acetate, propionate, butyrate, and valerate. These SCFAs serve as an energy source for host animals and play an important role in gut health. In this study, M. elsdenii was isolated from pig feces that had been collected from two farms located in distinct areas of Japan. These M. elsdenii isolates were genotyp ...
DOI:
10.1016/j.jbiosc.2017.12.016

51. Dietary Alfalfa and Calcium Salts of Long-Chain Fatty Acids Alter Protein Utilization, Microbial Populations, and Plasma Fatty Acid Profile in Holstein Freemartin Heifers

Author:
Yang He; Qinghua Qiu; Taoqi Shao; Wenjing Niu; Chuanqi Xia; Haibo Wang; Qianwen Li; Zhibiao Gao; Zhantao Yu; Huawei Su; Binghai Cao
Source:
Journal of agricultural and food chemistry 2017 v.65 no.50 pp. 10859-10867
ISSN:
1520-5118
Subject:
Butyrivibrio fibrisolvens; Holstein; Leymus chinensis; Megasphaera elsdenii; Protozoa; Ruminobacter amylophilus; Ruminococcus albus; Ruminococcus flavefaciens; Streptococcus bovis; alfalfa; ammonium nitrogen; calcium; crude protein; diet; digestibility; fatty acid composition; feed intake; finishing; heifers; lipid metabolism; long chain fatty acids; low density lipoprotein cholesterol; methanogens; microbial communities; neutral detergent fiber; nutrients; organic matter; polyunsaturated fatty acids; rumen fermentation; rumen fluids; salts; saturated fatty acids; triacylglycerols
Abstract:
... This study presented the effects of alfalfa and calcium salts of long-chain fatty acids (CSFA) on feed intake, apparent digestibility, rumen fermentation, microbial community, plasma biochemical parameters, and fatty acid profile in Holstein freemartin heifers. Eight Holstein freemartin heifers were randomly divided into a 4 × 4 Latin Square experiment with 2 × 2 factorial diets, with or without a ...
DOI:
10.1021/acs.jafc.7b04173

52. Effect of oral administration of Megasphaera elsdenii on performance of Holstein cows during early lactation

Author:
K.D. Stevens; M.L. Eastridge; K.M. Daniels; S.K. Finney; S.N. LeShure; J.L. Firkins
Source:
Professional animal scientists 2017 v.33 no.1 pp. 120-126
ISSN:
1080-7446
Subject:
Holstein; Megasphaera elsdenii; calving; corn; corn silage; dairy cows; dairy herds; dietary carbohydrate; early lactation; forage; gestation stalls; ketones; ketosis; milk; milk fat; milk yield; oral administration; pregnancy; reproductive performance; urine; wheat straw
Abstract:
... Primi- and multiparous Holstein cows (n = 162) at a commercial dairy herd were assigned to 1 of 4 treatments: control (no dose; n = 44); 200 mL of Lactipro (Megasphaera elsdenii, 1 × 108 cfu/mL; MSBiotec, Littleton, CO) when cows entered (~−14 DIM) the late gestation pen (n = 45); 200 mL of Lactipro at 1 to 3 d postpartum (n = 39); and dosed both prepartum and postpartum (n = 34). Late gestation c ...
DOI:
10.15232/pas.2016-01540

53. Effects of Saccharomyces cerevisiae fermentation products on performance and rumen fermentation and microbiota in dairy cows fed a diet containing low quality forage

Author:
Wen Zhu; Zihai Wei; Ningning Xu; Fan Yang; Ilkyu Yoon; Yihua Chung; Jianxin Liu; Jiakun Wang
Source:
Journal of animal science and biotechnology 2017 v.8 no.1 pp. 36
ISSN:
2049-1891
Subject:
Fibrobacter succinogenes; Holstein; Megasphaera elsdenii; Ruminococcus albus; Saccharomyces cerevisiae; Selenomonas ruminantium; Streptococcus bovis; acetates; agroindustrial byproducts; ammonium nitrogen; bacteria; butyrates; cellulolytic microorganisms; corn meal; corn stover; dairy cows; dairy industry; diet; dry matter intake; energy; fermentation; fermented foods; fungi; lactation; lactic acid; milk; milk composition; milk production; nutritive value; pH; propionic acid; protein synthesis; rumen; rumen fermentation; rumen fungi; volatile fatty acids
Abstract:
... BACKGROUND: A possible option to meet the increased demand of forage for dairy industry is to use the agricultural by-products, such as corn stover. However, nutritional value of crop residues is low and we have been seeking technologies to improve the value. A feeding trial was performed to evaluate the effects of four levels of Saccharomyces cerevisiae fermentation product (SCFP; Original XP; Di ...
DOI:
10.1186/s40104-017-0167-3
PubMed:
28465826
PubMed Central:
PMC5408399

54. Effects of the dose and viability of Saccharomyces cerevisiae. 1. Diversity of ruminal microbes as analyzed by Illumina MiSeq sequencing and quantitative PCR

Author:
Y. Jiang; I.M. Ogunade; S. Qi; T.J. Hackmann; C.R. Staples; A.T. Adesogan
Source:
Journal of dairy science 2017 v.100 no.1 pp. 325-342
ISSN:
0022-0302
Subject:
Bifidobacterium; Fibrobacter succinogenes; Lachnospiraceae; Megasphaera elsdenii; Ruminobacter; Ruminococcus flavefaciens; Saccharomyces cerevisiae; Selenomonas ruminantium; Streptococcus bovis; Treponema; animal performance; bacteria; cannulas; cattle feeds; cellulolytic microorganisms; corn silage; cow feeding; dairy cows; feed supplements; genes; grains; high-throughput nucleotide sequencing; lactating females; lactation; mechanism of action; milk; quantitative polymerase chain reaction; ribosomal RNA; rumen bacteria; rumen fermentation; species diversity; total mixed rations; yeasts
Abstract:
... This study was conducted to examine effects of the dose and viability of supplemental Saccharomyces cerevisiae on the ruminal fermentation and bacteria population and the performance of lactating dairy cows. Four ruminally cannulated lactating cows averaging 284±18d in milk were assigned to 4 treatments arranged in a 4×4 Latin square design with four 21-d periods. Cows were fed a total mixed ratio ...
DOI:
10.3168/jds.2016-11263

55. Preferential isolation of Megasphaera elsdenii from pig feces

Author:
Yu Kajihara; Shota Yoshikawa; Yuichiro Cho; Toshiyuki Ito; Hirokuni Miyamoto; Hiroaki Kodama
Source:
Anaerobe 2017 v.48 pp. 160-164
ISSN:
1075-9964
Subject:
DNA primers; Megasphaera elsdenii; agar; bacteria; beef extracts; culture media; fatty acid metabolism; feces; intestinal microorganisms; intestines; lactic acid; nucleotide sequences; polymerase chain reaction; ribosomal RNA; short chain fatty acids; swine
Abstract:
... Lactic acid produced by intestinal bacteria is fermented by lactate-utilizing bacteria. In this study, we developed a selective culture medium (KMI medium) for Megasphaera elsdenii, a lactate-utilizing bacterium that is abundant in pig intestines. Supplementation of the medium with lactate and beef extract powder was necessary for the preferential growth of M. elsdenii. In addition, we designed a ...
DOI:
10.1016/j.anaerobe.2017.08.013

56. Production of 1,3-diols in Escherichia coli

Author:
Naoya Kataoka; Alisa S. Vangnai; Thunyarat Pongtharangkul; Toshiharu Yakushi; Kazunobu Matsushita
Source:
Bioresource technology 2017 v.245 pp. 1538-1541
ISSN:
0960-8524
Subject:
Clostridium saccharoperbutylacetonicum; Cupriavidus necator; Escherichia coli; Megasphaera elsdenii; alcohol dehydrogenase; glucose; propionic acid
Abstract:
... To expand the diversity of chemical compounds produced through microbial conversion, a platform pathway for the production of widely used industrial chemicals, 1,3-diols, was engineered in Escherichia coli. The pathway was designed by modifying the previously reported (R)-1,3-butanediol synthetic pathway to consist of pct (propionate CoA-transferase) from Megasphaera elsdenii, bktB (thiolase), pha ...
DOI:
10.1016/j.biortech.2017.05.082

57. Biosynthesis of poly(glycolate-co-lactate-co-3-hydroxybutyrate) from glucose by metabolically engineered Escherichia coli

Author:
Zheng-Jun Li; Kangjian Qiao; Weichao Shi; Brian Pereira; Haoran Zhang; Bradley D. Olsen; Gregory Stephanopoulos
Source:
Metabolic engineering 2016 v.35 pp. 1-8
ISSN:
1096-7176
Subject:
3-hydroxybutyric acid; Cupriavidus necator; Escherichia coli; Megasphaera elsdenii; Pseudomonas; acetyl coenzyme A; biosynthesis; crystal structure; gene overexpression; glucose; glyoxylate cycle; lactic acid; mechanical properties; metabolic engineering; mutants; nuclear magnetic resonance spectroscopy; polyhydroxyalkanoates; polymerization
Abstract:
... Metabolically engineered Escherichia coli strains were constructed to effectively produce novel glycolate-containing biopolymers from glucose. First, the glyoxylate bypass pathway and glyoxylate reductase were engineered such as to generate glycolate. Second, glycolate and lactate were activated by the Megasphaera elsdenii propionyl-CoA transferase to synthesize glycolyl-CoA and lactyl-CoA, respec ...
DOI:
10.1016/j.ymben.2016.01.004

58. Chemical assembly of multiple metal cofactors: The heterologously expressed multidomain [FeFe]-hydrogenase from Megasphaera elsdenii

Author:
Giorgio Caserta; Agnieszka Adamska-Venkatesh; Ludovic Pecqueur; Mohamed Atta; Vincent Artero; Souvik Roy; Edward Reijerse; Wolfgang Lubitz; Marc Fontecave
Source:
Biochimica et biophysica acta 2016 v.1857 no.11 pp. 1734-1740
ISSN:
0005-2728
Subject:
Megasphaera elsdenii; biosynthesis; catalysts; ferredoxin hydrogenase; fuel cells; hydrogen; protons; rumen bacteria; spectroscopy
Abstract:
... [FeFe]-hydrogenases are unique and fascinating enzymes catalyzing the reversible reduction of protons into hydrogen. These metalloenzymes display extremely large catalytic reaction rates at very low overpotential values and are, therefore, studied as potential catalysts for bioelectrodes of electrolyzers and fuel cells. Since they contain multiple metal cofactors whose biosynthesis depends on comp ...
DOI:
10.1016/j.bbabio.2016.07.002

59. Conversion of l-lactate into n-caproate by a continuously fed reactor microbiome

Author:
Leo A. Kucek; Mytien Nguyen; Largus T. Angenent
Source:
Water research 2016 v.93 pp. 163-171
ISSN:
0043-1354
Subject:
Megasphaera elsdenii; batch systems; beta oxidation; bioreactors; carbohydrates; lactic acid; liquid-liquid extraction; microbiome; pH; propionic acid; wastewater
Abstract:
... Conversion of lactate to n-caproate had been described for the type strain Megasphaera elsdenii in batch systems. Recently, investigators have also described production of n-caproate from endogenous or exogenous lactate with batch-fed reactor microbiome systems. However, no reports exist of lactate to n-caproate conversion within a continuously fed bioreactor. Since continuously fed systems are ad ...
DOI:
10.1016/j.watres.2016.02.018

60. Effects of supplementation of active dried yeast and malate during sub-acute ruminal acidosis on rumen fermentation, microbial population, selected blood metabolites, and milk production in dairy cows

Author:
M. Malekkhahi; A.M. Tahmasbi; A.A. Naserian; M. Danesh-Mesgaran; J.L. Kleen; O. AlZahal; M.H. Ghaffari
Source:
Animal feed science and technology 2016 v.213 pp. 29-43
ISSN:
0377-8401
Subject:
Fibrobacter succinogenes; Holstein; Megasphaera elsdenii; Saccharomyces cerevisiae; Streptococcus bovis; ammonium nitrogen; blood; body weight; dairy cows; dietary supplements; dry matter intake; glucose; insulin; lactation; malates; metabolites; milk yield; pH; rumen; rumen fermentation; rumen protozoa; ruminal acidosis; yeasts
Abstract:
... The objective of this study was to evaluate the effects of dietary supplementation of malate (MAL) and active dried yeast (ADY) on feed intake, rumen fermentation parameters, rumen microbial populations, selected blood metabolites, and milk production during a sub-acute ruminal acidosis (SARA) challenge in primiparous lactating dairy cows. Six rumen-fistulated Holstein dairy cows (body weight: 630 ...
DOI:
10.1016/j.anifeedsci.2015.12.018

61. Effects of virginiamycin and monensin administered alone or together with Megasphaera elsdenii strain NCIMB 41125 on in vitro production of lactate and VFA and the effects of monensin and M. elsdenii strain NCIMB 41125 on health and performance of feedlot steers

Author:
K.-J. Leeuw; H.H. Meissner; P.H. Henning; F.K. Siebrits; J.H.A. Apajalahti; A. Kettunen
Source:
Livestock science 2016 v.183 pp. 54-62
ISSN:
1871-1413
Subject:
Bonsmara; Megasphaera elsdenii; animal performance; bacteria; butyrates; carcass weight; diet; dressing percentage; feed concentrates; feed conversion; fermentation; finishing; lactic acid; liver; men; monensin; morbidity; mortality; pH; plate count; propionic acid; rumen; sequence analysis; steers; virginiamycin
Abstract:
... Virginiamycin (VIR) and monensin (MON) are included in high concentrate diets to mitigate digestive disturbances and to increase performance of ruminants in intensive systems. Megasphaera elsdenii strain NCIMB 41125 (Me) rapidly utilises lactate in the rumen, thereby controlling ruminal pH and digestive disturbances. Me promotes butyrate and valerate production whereas VIR and MON promote propiona ...
DOI:
10.1016/j.livsci.2015.11.014

62. Fermentation characteristics of Megasphaera elsdenii J6 derived from pig feces on different lactate isomers

Author:
Xiao-lin JIANG; Yong SU; Wei-yun ZHU
Source:
Journal of integrative agriculture 2016 v.15 no.7 pp. 1575-1583
ISSN:
2095-3119
Subject:
Bacteroides acidifaciens; Bacteroides fragilis; Bacteroides uniformis; Megasphaera elsdenii; Veillonella; acidosis; anaerobes; bacteria; butyrates; carbon; digestive system; feces; fermentation; isomers; lactic acid; nucleotide sequences; ribosomal RNA; short chain fatty acids; swine
Abstract:
... D-Lactate-utilizing bacteria play important roles in maintaining the balance of gut lactate; however, studies on gut D-lactate-utilizing bacteria have been limited. This study aimed to isolate and identify D-lactate-utilizing bacteria from pig gut using the Hungate roll-tube method, and to investigate their metabolic characteristics in vitro. Six different anaerobes were isolated from pig feces, w ...
DOI:
10.1016/S2095-3119(15)61236-9

63. Impacts of ruminal microorganisms on the production of fuels: how can we intercede from the outside?

Author:
Paul J. Weimer; Richard A. Kohn
Source:
Applied microbiology and biotechnology 2016 v.100 no.8 pp. 3389-3398
ISSN:
0175-7598
Subject:
Clostridium kluyveri; Megasphaera elsdenii; alkanes; anaerobic digesters; bacteria; biomass; ethanol; fermentation; fuels; hexanoic acid; methane production; methanogens; microbial communities; microbiome; mixed culture; rumen microorganisms; ruminants; sediments; species diversity; thermodynamics
Abstract:
... The ruminal microbiome rapidly converts plant biomass to short-chain fatty acids (SCFA) that nourish the ruminant animal host. Because of its high species diversity, functional redundancy, and ease of extraruminal cultivation, this mixed microbial community is a particularly accomplished practitioner of the carboxylate platform for producing fuels and chemical precursors. Unlike reactor microbiome ...
Handle:
10113/62657
DOI:
10.1007/s00253-016-7358-2
CHORUS:
10.1007/s00253-016-7358-2

64. Parameters of fermentation and rumen microbiota of Nellore steers fed with different proportions of concentrate in fresh sugarcane containing diets

Author:
Carlos S. Ribeiro Júnior; Juliana D. Messana; Yury T. Granja-Salcedo; Roberta C. Canesin; Giovani Fiorentini; Elias San Vito; Luiz R. Furlan; Ricardo A. Reis; Telma T. Berchielli
Source:
Archives of animal nutrition 2016 v.70 no.5 pp. 402-415
ISSN:
1477-2817
Subject:
Entodinium; Fibrobacter succinogenes; Megasphaera elsdenii; Nellore; Protozoa; Selenomonas ruminantium; bacteria; diet; digestibility; nutrients; organic matter; pH; population growth; residual effects; rumen; rumen fermentation; rumen microorganisms; steers; sugarcane; valeric acid
Abstract:
... The objective of this study was to evaluate the effect of a fresh sugarcane-based diet and different roughage-to-concentrate ratios (70:30, 60:40, 40:60 and 20:80) on the rumen microbiota associated with rumen fermentation parameters and the intake and apparent digestibility of nutrients in Nellore steers. Eight rumen-cannulated Nellore steers (331 ± 8 kg BW) were distributed in a double 4 × 4 Lat ...
DOI:
10.1080/1745039X.2016.1206737

65. Rumen microbial abundance and fermentation profile during severe subacute ruminal acidosis and its modulation by plant derived alkaloids in vitro

Author:
Elsayed Mickdam; Ratchaneewan Khiaosa-ard; Barbara U. Metzler-Zebeli; Fenja Klevenhusen; Remigius Chizzola; Qendrim Zebeli
Source:
Anaerobe 2016 v.39 pp. 4-13
ISSN:
1075-9964
Subject:
Entodinium; Fibrobacter succinogenes; Lactobacillus; Megasphaera elsdenii; Methanobrevibacter; Prevotella; Protozoa; Ruminobacter amylophilus; Ruminococcus albus; animals; bacteria; bioactive compounds; biochemical pathways; crude protein; diet; fermentation; fungi; genes; methane; methane production; methanogens; microbial communities; models; pH; principal component analysis; propionic acid; ribosomal RNA; rumen fermentation; rumen microorganisms; ruminal acidosis; sanguinarine; short chain fatty acids
Abstract:
... Rumen microbiota have important metabolic functions for the host animal. This study aimed at characterizing changes in rumen microbial abundances and fermentation profiles using a severe subacute ruminal acidosis (SARA) in vitro model, and to evaluate a potential modulatory role of plant derived alkaloids (PDA), containing quaternary benzophenanthridine and protopine alkaloids, of which sanguinari ...
DOI:
10.1016/j.anaerobe.2016.02.002

66. Abundance of ruminal bacteria, epithelial gene expression, and systemic biomarkers of metabolism and inflammation are altered during the peripartal period in dairy cows

Author:
A. Minuti; A. Palladino; M.J. Khan; S. Alqarni; A. Agrawal; F. Piccioli-Capelli; F. Hidalgo; F.C. Cardoso; E. Trevisi; J.J. Loor
Source:
Journal of dairy science 2015 v.98 no.12 pp. 8940-8951
ISSN:
0022-0302
Subject:
3-hydroxybutyric acid; Anaerovibrio lipolyticus; Butyrivibrio; Fibrobacter succinogenes; Holstein; Megasphaera elsdenii; Prevotella bryantii; Toll-like receptor 2; Toll-like receptor 4; acetates; albumins; bacteria; bilirubin; biomarkers; biopsy; blood; butyrates; calving; cell proliferation; dairy cows; diet; digesta; endotoxins; energy balance; fistula; free fatty acids; gene expression; genes; glucose; immune system; inflammation; insulin resistance; lactation; liver function; metabolism; milk; pH; reverse transcriptase polymerase chain reaction; rumen bacteria; rumen epithelium; transporters
Abstract:
... Seven multiparous Holstein cows with a ruminal fistula were used to investigate the changes in rumen microbiota, gene expression of the ruminal epithelium, and blood biomarkers of metabolism and inflammation during the transition period. Samples of ruminal digesta, biopsies of ruminal epithelium, and blood were obtained during −14 through 28d in milk (DIM). A total of 35 genes associated with meta ...
DOI:
10.3168/jds.2015-9722

67. Direct-fed microbes: A tool for improving the utilization of low quality roughages in ruminants

Author:
Mona M Y Elghandour; Abdelfattah Z M Salem; Jose S Martínez Castañeda; Luis M Camacho; Ahmed E Kholif; Juan C Vázquez Chagoyán
Source:
Journal of integrative agriculture 2015 v.14 no.3 pp. 526-533
ISSN:
2095-3119
Subject:
Aspergillus; Bifidobacterium; Enterococcus; Lactobacillus; Megasphaera elsdenii; Propionibacterium; Saccharomyces; Streptococcus; antibiotics; bacteria; diet; digestibility; dry matter intake; feed conversion; immune system; lactic acid; lipid content; mechanism of action; microbial ecology; milk fat yield; oxygen; pathogens; rumen; rumen fermentation; ruminants; weight gain; yeasts
Abstract:
... For many years, ruminant nutritionists and microbiologists have been interested in manipulating the microbial ecosystem of the rumen to improve production efficiency of different ruminant species. Removal and restriction of antibiotics sub-therapeutic uses from ruminant diets has amplified interest in improving nutrient utilization and animal performance and search for more safe alternatives. Some ...
DOI:
10.1016/S2095-3119(14)60834-0

68. Effect of Megasphaera elsdenii NCIMB 41125 dosing on rumen development, volatile fatty acid production and blood β‐hydroxybutyrate in neonatal dairy calves

Author:
M. C. Muya; F. V. Nherera; K. A. Miller; C. C. Aperce; P. M. Moshidi; L. J. Erasmus
Source:
Journal of animal physiology and animal nutrition 2015 v.99 no.5 pp. 913-918
ISSN:
0931-2439
Subject:
3-hydroxybutyric acid; Megasphaera elsdenii; acetates; average daily gain; blood; body weight; butyrates; colostrum; dairy calves; dry matter intake; epithelium; freshwater; intestinal absorption; males; metabolism; propionic acid; rumen; rumen development; volatile fatty acids; weaning; whole milk
Abstract:
... Thirty calves were randomly assigned to two treatments and fed until weaning [42 days (d) of age]. Treatments were a control group (n = 15), which did not receive Megasphaera elsdenii (Me0) and a M. elsdenii group, which received a 50‐ml oral dose of M. elsdenii NCIMB 41125 (10⁸ CFU/ml) at day 14 day of age (Me14). Calves were given colostrum for the first 3 day followed by limited whole milk feed ...
DOI:
10.1111/jpn.12306

69. Effects of different dietary concentrate to forage ratio and thiamine supplementation on the rumen fermentation and ruminal bacterial community in dairy cows

Author:
Hongrong Wang; Xiaohua Pan; Chao Wang; Mengzhi Wang; Lihuai Yu
Source:
Animal production science 2015 v.55 no.2 pp. 189-193
ISSN:
1836-0939
Subject:
Butyrivibrio; Holstein; Lactobacillus; Megasphaera elsdenii; Selenomonas ruminantium; Streptococcus bovis; bacteria; bacterial communities; dairy cows; diet; models; pH; rumen; rumen bacteria; rumen fermentation; ruminal acidosis; thiamin
Abstract:
... A subacute ruminal acidosis (SARA) model was induced gradually by increasing the proportion of dietary concentrate to evaluate the effect of thiamine supplementation on the structure of bacterial community in dairy cows. Three Holstein dairy cows with rumen cannula were randomly assigned to a replicated 3 × 3 Latin square design trial and received three diets during three successive 21-day periods ...
DOI:
10.1071/AN14523

70. Effects of ruminal dosing of Holstein cows with Megasphaera elsdenii on milk fat production, ruminal chemistry, and bacterial strain persistence1

Author:
P.J. Weimer; L. Da Silva Cabral; F. Cacite
Source:
Journal of dairy science 2015 v.98 no.11 pp. 8078-8092
ISSN:
0022-0302
Subject:
Holstein; Megasphaera elsdenii; alfalfa silage; bacteria; butyrates; corn; corn oil; corn silage; cow feeding; cows; gene dosage; genes; herds; lactation stage; lactic acid; milk fat; milk fat yield; pH; polymerase chain reaction; protein supplements; ribosomal RNA; rumen; rumen bacteria; starch; volatile fatty acids
Abstract:
... Megasphaera elsdenii is a lactate-utilizing bacterium whose ruminal abundance has been shown to be greatly elevated during milk fat depression (MFD). To further examine this association, a total of 23 cannulated multiparous Holstein cows were examined in 3 experiments in which strains of M. elsdenii were directly dosed into the rumen (~2×1012 cells/dose); control cows were dosed with sterile lacta ...
Handle:
10113/62004
DOI:
10.3168/jds.2015-9721
CHORUS:
10.3168/jds.2015-9721

71. Feeding increasing concentrate to Tifton 85 hay ratios modulated rumen fermentation and microbiota in Nellore feedlot steers

Author:
C. S. RIBEIRO; Y. T. GRANJA-SALCEDO; J. D. MESSANA; A. J. NETO; R. C. CANESIN; G. FIORENTINI; M. F. F. ALARCON; T. T. BERCHIELLI
Source:
Journal of agricultural science 2015 v.153 no.6 pp. 1116-1127
ISSN:
1469-5146
Subject:
Dasytricha; Fibrobacter succinogenes; Lactobacillus; Megasphaera elsdenii; Nellore; Protozoa; Ruminococcus albus; Ruminococcus flavefaciens; Selenomonas ruminantium; Streptococcus bovis; ammonium nitrogen; bacteria; cellulolytic microorganisms; diet; digestibility; energy; feedlots; hay; lactic acid; nitrogen; organic matter; pH; propionic acid; rumen; rumen fermentation; rumen microorganisms; steers; volatile fatty acids
Abstract:
... The use of diets with increasing proportions of concentrate to fibre can ensure appropriate energy levels and result in greater efficiency in Nellore feedlot steers. It was hypothesized that higher proportions of concentrate in the diet of these Nellore steers may affect ruminal fermentation and microbiota as a consequence of ruminal pH reduction. The present study was conducted to evaluate the ef ...
DOI:
10.1017/S0021859615000337

72. LC-MS/MS-based analysis of coenzyme A and short-chain acyl-coenzyme A thioesters

Author:
Stefan Neubauer; Dinh Binh Chu; Hans Marx; Michael Sauer; Stephan Hann; Gunda Koellensperger
Source:
Analytical and bioanalytical chemistry 2015 v.407 no.22 pp. 6681-6688
ISSN:
1618-2642
Subject:
Megasphaera elsdenii; coenzyme A; exercise; isomers; liquid chromatography; mass spectrometry; metabolites; rumen bacteria; yeasts
Abstract:
... Absolute quantification of intracellular coenzyme A (CoA), coenzyme A disulfide, and short-chain acyl-coenzyme A thioesters was addressed by developing a tailored metabolite profiling method based on liquid chromatography in combination with tandem mass spectrometric detection (LC-MS/MS). A reversed phase chromatographic separation was established which is capable of separating a broad spectrum of ...
DOI:
10.1007/s00216-015-8825-9

73. Microbial populations and fermentation profiles in rumen liquid and solids of Holstein cows respond differently to dietary barley processing

Author:
B.U. Metzler‐Zebeli; A. Khol‐Parisini; L. Gruber; Q. Zebeli
Source:
Journal of applied microbiology 2015 v.119 no.6 pp. 1502-1514
ISSN:
1364-5072
Subject:
Fibrobacter succinogenes; Holstein; Lactobacillus; Megasphaera elsdenii; Ruminobacter amylophilus; Ruminococcus albus; Ruminococcus flavefaciens; barley; butyrates; dairy cows; diet; energy; fermentation; fungi; gluconeogenesis; heat; lactic acid; pH; propionic acid; rumen; rumen fermentation; volatile fatty acids
Abstract:
... AIMS: To evaluate the effects of treating barley grain with lactic acid (LA) and heat on postprandial dynamics of 19 microbial taxa and fermentation in the rumen of dairy cows. METHODS AND RESULTS: This study was designed as a double 3 × 3 Latin square with six rumen‐cannulated cows and three diets either containing untreated control barley or barley treated with 1% LA and 1% LA and heat (LAH, 55° ...
DOI:
10.1111/jam.12958

74. Partially replacing cornstarch in a high-concentrate diet with sucrose inhibited the ruminal trans-10 biohydrogenation pathway in vitro by changing populations of specific bacteria

Author:
Xiaoqin Sun; Yaping Wang; Bo Chen; Xin Zhao
Source:
Journal of animal science and biotechnology 2015 v.6 no.1 pp. 57
ISSN:
2049-1891
Subject:
Butyrivibrio fibrisolvens; Megasphaera elsdenii; acetates; biohydrogenation; biotechnology; butyrates; corn starch; dietary carbohydrate; feed concentrates; fermentation; forage; milk fat; pH; propionic acid; starch; sucrose
Abstract:
... BACKGROUND: The positive influence of replacing dietary starch with sugar on milk fat production has been proposed to be partially attributed to the inhibition of the rumen trans-10 biohydrogenation pathway. However, whether and how sucrose inhibits the rumen trans-10 biohydrogenation pathway remains elusive. RESULTS: A batch in vitro incubation system was used to evaluate effects of replacing cor ...
DOI:
10.1186/s40104-015-0051-y

75. Rapid changes in key ruminal microbial populations during the induction of and recovery from diet-induced milk fat depression in dairy cows

Author:
D. E. Rico; S. H. Preston; J. M. Risser; K. J. Harvatine
Source:
British journal of nutrition 2015 v.114 no.3 pp. 358-367
ISSN:
1475-2662
Subject:
Butyrivibrio fibrisolvens; Fibrobacter succinogenes; Megasphaera elsdenii; Prevotella bryantii; Protozoa; Pseudobutyrivibrio; Selenomonas ruminantium; Streptococcus bovis; bacteria; dairy cows; fatty acids; fungi; gastrointestinal system; microbial communities; milk fat yield; models; temporal variation
Abstract:
... The ruminant provides a powerful model for understanding the temporal dynamics of gastrointestinal microbial communities. Diet-induced milk fat depression (MFD) in the dairy cow is caused by rumen-derived bioactive fatty acids, and is commonly attributed to the changes in the microbial population. The aim of the present study was to determine the changes occurring in nine ruminal bacterial taxa wi ...
DOI:
10.1017/S0007114515001865
PubMed:
26123320

76. Reduction of ferredoxin or oxygen by flavin‐based electron bifurcation in Megasphaera elsdenii

Author:
Nilanjan P. Chowdhury; Jörg Kahnt; Wolfgang Buckel
Source:
FEBS journal 2015 v.282 no.16 pp. 3149-3160
ISSN:
1742-464X
Subject:
Acidaminococcus fermentans; Megasphaera elsdenii; NAD (coenzyme); adenosine monophosphate; aerobic conditions; anaerobes; anaerobic conditions; bacteria; flavoproteins; hydrogen peroxide; oxygen; rumen bacteria; spectral analysis; stoichiometry
Abstract:
... Over 50 years ago, it was reported that, in the anaerobic rumen bacterium Megasphaera elsdenii, the reduction of crotonyl‐CoA to butyryl‐CoA by NADH involved an electron transferring flavoprotein (Etf) as mediator [Baldwin RL, Milligan LP (1964) Biochim Biophys Acta 92, 421–432]. Purification and spectroscopic characterization revealed that this Etf contained 2 FAD, whereas, in the Etfs from aerob ...
DOI:
10.1111/febs.13308

77. Responses in ileal and cecal bacteria to low and high amylose/amylopectin ratio diets in growing pigs

Author:
Yu-heng Luo; Can Yang; André-Denis G. Wright; Jun He; Dai-wen Chen
Source:
Applied microbiology and biotechnology 2015 v.99 no.24 pp. 10627-10638
ISSN:
0175-7598
Subject:
Actinobacillus minor; Escherichia fergusonii; Halomonas pacifica; Lactobacillus; Megasphaera elsdenii; Mitsuokella multacida; Prevotella; acetates; amylopectin; amylose; bacteria; bacterial communities; butyrates; carbon; diet; digesta; digestion; fermentation; ileum; intestinal microorganisms; metabolites; nutrients; pathogens; probiotics; risk; sequence analysis; short chain fatty acids; swine; swine feeding
Abstract:
... Dietary starch that escapes digestion in the small intestine may serve as a carbon source for bacterial fermentation in the distal intestine. This study aimed to compare the bacterial community in the ileal and cecal digesta of growing pigs fed diets with low (0.14, LR pigs) and high (0.43, HR pigs) amylose/amylopectin ratio. Pyrosequencing based on MiSeq 2000 platform showed that in ileum digesta ...
DOI:
10.1007/s00253-015-6917-2

78. Treatment of grain with organic acids at 2 different dietary phosphorus levels modulates ruminal microbial community structure and fermentation patterns in vitro

Author:
H. Harder; A. Khol-Parisini; B.U. Metzler-Zebeli; F. Klevenhusen; Q. Zebeli
Source:
Journal of dairy science 2015 v.98 no.11 pp. 8107-8120
ISSN:
0022-0302
Subject:
Prevotella; ammonia; acetates; inorganic phosphorus; Protozoa; short chain fatty acids; propionic acid; lactic acid; fermentation; barley; Ruminococcus albus; rumen microorganisms; butyrates; phytic acid; community structure; anaerobes; fermenters; microbial communities; Megasphaera elsdenii; hydrolysis; saliva; quantitative polymerase chain reaction; diet; neutral detergent fiber; rumen fluids; bacteria; fungi
Abstract:
... Recent data indicate positive effects of treating grain with citric (CAc) or lactic acid (LAc) on the hydrolysis of phytate phosphorus (P) and fermentation products of the grain. This study used a semicontinuous rumen simulation technique to evaluate the effects of processing of barley with 50.25g/L (wt/vol) CAc or 76.25g/L LAc on microbial composition, metabolic fermentation profile, and nutrient ...
DOI:
10.3168/jds.2015-9913

79. Active dry Saccharomyces cerevisiae can alleviate the effect of subacute ruminal acidosis in lactating dairy cows

Author:
O. AlZahal; L. Dionissopoulos; A.H. Laarman; N. Walker; B.W. McBride
Source:
Journal of dairy science 2014 v.97 no.12 pp. 7751-7763
ISSN:
0022-0302
Subject:
Anaerovibrio lipolyticus; Fibrobacter succinogenes; Gram-negative bacteria; Holstein; Megasphaera elsdenii; Prevotella albensis; Ruminococcus albus; Saccharomyces cerevisiae; Streptococcus bovis; anaerobes; cellulolytic microorganisms; dairy cows; digesta; dry matter intake; fungi; inflammation; lactating females; lactic acid; lipopolysaccharides; microbial communities; milk composition; milk consumption; milk yield; pH; propionic acid; quantitative polymerase chain reaction; rumen; rumen fermentation; ruminal acidosis; volatile fatty acids
Abstract:
... The objective of the study was to determine the effect of active dry Saccharomyces cerevisiae (ADSC) supplementation on dry matter intake, milk yield, milk components, ruminal pH, and microbial community during a dietary regimen that leads to subacute ruminal acidosis (SARA). Sixteen multiparous, rumen-cannulated lactating Holstein cows were randomly assigned to 1 of 2 dietary treatments that incl ...
DOI:
10.3168/jds.2014-8212

80. Changes of rumen pH, fermentation and microbial population as influenced by different ratios of roughage (rice straw) to concentrate in dairy steers

Author:
M. WANAPAT; P. GUNUN; N. ANANTASOOK; S. KANG
Source:
Journal of agricultural science 2014 v.152 no.4 pp. 675-685
ISSN:
1469-5146
Subject:
Butyrivibrio fibrisolvens; Fibrobacter succinogenes; Megasphaera elsdenii; Protozoa; RNA; acetates; acid detergent fiber; body weight; cellulolytic microorganisms; conjugated linoleic acid; crates; crude protein; diet; dry matter intake; ecology; energy intake; fermentation; fungi; neutral detergent fiber; organic matter; pH; propionic acid; protein synthesis; proteolytic bacteria; quantitative polymerase chain reaction; rice straw; rumen; rumen fermentation; steers; urine; volatile fatty acids
Abstract:
... The current study was designed to determine the effect of roughage to concentrate ratio (R : C) on rumen pH, fermentation and bacterial population in dairy steers. Four rumen fistulated dairy steers (170±20 kg) were randomly assigned according to a 4×4 Latin square design, in which the steers were fed with four dietary treatments with different R : C ratios of 0·8 : 0·2, 0·6 : 0·4, 0·4 : 0·6 and 0 ...
DOI:
10.1017/S0021859613000658

81. Effects of the acid-tolerant engineered bacterial strain Megasphaera elsdenii H6F32 on ruminal pH and the lactic acid concentration of simulated rumen acidosis in vitro

Author:
M. Long; W.J. Feng; P. Li; Y. Zhang; R.X. He; L.H. Yu; J.B. He; W.Y. Jing; Y.M. Li; Z. Wang; G.W. Liu
Source:
Research in veterinary science 2014 v.96 no.1 pp. 28-29
ISSN:
0034-5288
Subject:
Megasphaera elsdenii; acid tolerance; lactic acid; mixed culture; pH; rumen bacteria; ruminal acidosis
Abstract:
... The aim of this study was to examine the effects of the acid-tolerant engineered bacterial strain Megasphaera elsdenii H6F32 (M. elsdenii H6F32) on ruminal pH and the lactic acid concentrations in simulated rumen acidosis conditions in vitro. A mixed culture of ruminal bacteria, buffer, and primarily degradable substrates was inoculated with equal numbers of M. elsdenii H6 or M. elsdenii H6F32. Th ...
DOI:
10.1016/j.rvsc.2013.11.013

82. Effects of vanillin, quillaja saponin, and essential oils on in vitro fermentation and protein-degrading microorganisms of the rumen

Author:
Amlan K. Patra; Zhongtang Yu
Source:
Applied microbiology and biotechnology 2014 v.98 no.2 pp. 897-905
ISSN:
0175-7598
Subject:
Butyrivibrio fibrisolvens; Clostridium aminophilum; Clostridium sticklandii; Megasphaera elsdenii; Origanum; Prevotella bryantii; Prevotella ruminicola; Ruminobacter amylophilus; Ruminococcus flavefaciens; Selenomonas ruminantium; ammonia; bacteria; clove oil; fermentation; methane production; peppermint oil; rumen fermentation; rumen microorganisms; vanillin; volatile fatty acids
Abstract:
... This study investigated the effects of vanillin on methanogenesis and rumen fermentation, and the responses of ruminal protein-degrading bacteria to vanillin (at concentrations of 0, 0.76 and 1.52 g/L), essential oils (clove oil, 1 g/L; origanum oil, 0.50 g/L, and peppermint oil, 1 g/L), and quillaja saponin (at concentration of 0 and 6 g/L) in vitro. Methane production, degradabilities of feed su ...
DOI:
10.1007/s00253-013-4930-x
PubMed:
23624710

83. Efficacy and mode of action of selected non-ionophore antibiotics and direct-fed microbials in relation to Megasphaera elsdenii NCIMB 41125 during in vitro fermentation of an acidosis-causing substrate

Author:
H.H. Meissner; P.H. Henning; K.-J. Leeuw; F.M. Hagg; C.H. Horn; A. Kettunen; J.H.A. Apajalahti
Source:
Livestock science 2014 v.162 pp. 115-125
ISSN:
1871-1413
Subject:
Megasphaera elsdenii; Saccharomyces cerevisiae; bacitracin; bacteria; bambermycin; butyrates; fermentation; hydrolysates; in vitro studies; lactic acid; mechanism of action; oxytetracycline; propionic acid; ruminal acidosis; tylosin; yeasts; zinc
Abstract:
... The efficacy of prominent in-feed antibiotics and direct-fed microbials (DFM) to prevent or mitigate ruminal acidosis and lactate accumulation, in addition to whether their presence will enhance or inhibit Megasphaera elsdenii strain NCIMB 41125 (Me) were studied in vitro. The antibiotics studied were aureomycin+sulfamethazine as AS-700 (AS), terramycin as TM-200 (TM), zinc bacitracin (ZB), flavom ...
DOI:
10.1016/j.livsci.2014.01.026

84. Fermentable non-starch polysaccharides increases the abundance of Bacteroides–Prevotella–Porphyromonas in ileal microbial community of growing pigs

Author:
E. Ivarsson; S. Roos; H. Y. Liu; J. E. Lindberg
Source:
Animal 2014 v.8 no.11 pp. 1777-1787
ISSN:
1751-732X
Subject:
Enterobacteriaceae; Lactobacillus reuteri; Megasphaera elsdenii; acetic acid; arabinose; arabinoxylan; butyric acid; cannulas; chicory; correlation; dietary fiber; digesta; feces; fermentation; forage; genes; grasses; ileum; ingredients; intestinal microorganisms; lactic acid; microbial communities; pectins; quantitative polymerase chain reaction; sugar beet pulp; swine; swine feeding; swine production; wheat bran; xylan 1,4-beta-xylosidase; xylose
Abstract:
... Most plant-origin fiber sources used in pig production contains a mixture of soluble and insoluble non-starch polysaccharides (NSP). The knowledge about effects of these sources of NSP on the gut microbiota and its fermentation products is still scarce. The aim of this study was to investigate effects of feeding diets with native sources of NSP on the ileal and fecal microbial composition and the ...
DOI:
10.1017/S1751731114001827
PubMed:
25046106

85. Impact of organic loading rate on biohydrogen production in an up-flow anaerobic packed bed reactor (UAnPBR)

Author:
Antônio Djalma Nunes Ferraz Júnior; Marcelo Zaiat; Medhavi Gupta; Elsayed Elbeshbishy; Hisham Hafez; George Nakhla
Source:
Bioresource technology 2014 v.164 pp. 371-379
ISSN:
0960-8524
Subject:
Clostridium beijerinckii; Clostridium butyricum; Megasphaera elsdenii; Propionispira arboris; anaerobic digesters; biomass; denaturing gradient gel electrophoresis; glucose; hydrogen; hydrogen production; polymerase chain reaction
Abstract:
... This study assesses the impact of organic loading rate on biohydrogen production from glucose in an up-flow anaerobic packed bed reactor (UAnPBR). Two mesophilic UAPBRs (UAnPBR1 and 2) were tested at organic loading rates (OLRs) ranging from 6.5 to 51.4gCODL−1d−1. To overcome biomass washout, design modifications were made in the UAnPBR2 to include a settling zone to capture the detached biomass. ...
DOI:
10.1016/j.biortech.2014.05.011

86. Megasphaera indica sp. nov., an obligate anaerobic bacteria isolated from human faeces

Author:
V. B. Lanjekar; N. P. Marathe; V. Venkata Ramana; Y. S. Shouche; D. R. Ranade
Source:
International journal of systematic and evolutionary microbiology 2014 v.64 no.Pt 7 pp. 2250-2256
ISSN:
1466-5034
Subject:
Clostridium; DNA; Gram-negative bacteria; Megasphaera elsdenii; Sporomusa; carbon; cellobiose; energy; feces; fermentation; galactose; glucose; hexanoic acid; humans; lactose; nucleic acid hybridization; nucleotide sequences; phylogeny; rhamnose; ribosomal RNA; sequence homology; succinic acid; sucrose; temperature; volatile fatty acids; volunteers
Abstract:
... Two coccoid, non-motile, obligately anaerobic, Gram-stain-negative bacteria, occurring singly or in pairs, or as short chains, with a mean size of 1.4–2.5 µm were isolated from the faeces of two healthy human volunteers, aged 26 and 56 years, and were designated NMBHI-10T and BLPYG-7, respectively. Both the strains were affiliated to the sub-branch Sporomusa of the class Clostridia as revealed by ...
DOI:
10.1099/ijs.0.059816-0

87. Organic loading rate impact on biohydrogen production and microbial communities at anaerobic fluidized thermophilic bed reactors treating sugarcane stillage

Author:
Samantha Christine Santos; Paula Rúbia Ferreira Rosa; Isabel Kimiko Sakamoto; Maria Bernadete Amâncio Varesche; Edson Luiz Silva
Source:
Bioresource technology 2014 v.159 pp. 55-63
ISSN:
0960-8524
Subject:
Lactobacillus; Megasphaera; butyric acid; fermenters; fluidized beds; hydrogen production; lactic acid; microbial communities; sugarcane
Abstract:
... This study aimed to evaluate the effect of high organic loading rates (OLR) (60.0–480.00kgCODm−3d−1) on biohydrogen production at 55°C, from sugarcane stillage for 15,000 and 20,000mgCODL−1, in two anaerobic fluidized bed reactors (AFBR1 and AFBR2). It was obtained, for H2 yield and content, a decreasing trend by increasing the OLR. The maximum H2 yield was observed in AFBR1 (2.23mmolg CODadded-1) ...
DOI:
10.1016/j.biortech.2014.02.051

88. Synthesis and Biological Evaluation of 7α,7α,7α,8α,8α,8α- Hexafluororiboflavin and 7α,7α,7α,8α,8α,8α-Hexafluoro-FMN

Author:
Peter Nielsen; Adelbert Bacher; Diana Darling; Mark Cushman
Source:
Zeitschrift für Naturforschung 2014 v.38 no.9-10 pp. 701-707
ISSN:
1865-7125
Subject:
Bacillus subtilis; Lactobacillus casei; Megasphaera elsdenii; Photobacterium; dissociation; electrodes; hydrogen; luciferase; redox potential; riboflavin
Abstract:
... 7α,7α,7α,8α,8α,8α-Hexafluororiboflavin (4) has been prepared and its oxidation-reduction potential determined polarographically to be + 0.02 V with respect to the standard hydrogen electrode. Compound 4 does not by itself promote the growth of Lactobacillus casei. However, in the presence of low riboflavin (1) concentrations, the hexafluoro analog 4 has some growth enhancing activity. The FMN anal ...
DOI:
10.1515/znc-1983-9-1006

89. The effect of Bioflavex® and its pure flavonoid components on in vitro fermentation parameters and methane production in rumen fluid from steers given high concentrate diets

Author:
A.R. Seradj; L. Abecia; J. Crespo; D. Villalba; M. Fondevila; J. Balcells
Source:
Animal feed science and technology 2014 v.197 pp. 85-91
ISSN:
0377-8401
Subject:
Megasphaera elsdenii; Methanosarcina; Selenomonas ruminantium; Streptococcus bovis; acetates; ammonium nitrogen; bacteria; bottles; feed concentrates; fermentation; flavonoids; in vitro studies; inoculum; lactic acid; methane; methane production; methanogens; pH; propionic acid; quantitative polymerase chain reaction; rumen fermentation; rumen fluids; steers; volatile fatty acids
Abstract:
... An in vitro assay was designed to analyze the effect of either Bioflavex® (BF) or each of its pure flavonoid components [Neoeriocitrine (NE), Naringine (NG), Isonaringine (IN), Hesperidine (HS), Neohesperidine (NH), Poncirine (PC)] added at 200μg/g dry matter (DM) incubated substrate on rumen fermentation, methane production (CH4) and microbial population. A treatment without flavonoids was also i ...
DOI:
10.1016/j.anifeedsci.2014.08.013

90. Effects of Increasing Non-Fiber Carbohydrate to Neutral Detergent Fiber Ratio on Rumen Fermentation and Microbiota in Goats

Author:
Da-cheng LIU; Xiang-li ZHOU; Pei-ting ZHAO; Min GAO; Hao-qi HAN; Hong-lian HU
Source:
Journal of integrative agriculture 2013 v.12 no.2 pp. 319-326
ISSN:
2095-3119
Subject:
Lactobacillus; Megasphaera elsdenii; Protozoa; Streptococcus bovis; bacteria; butyrates; fluorescence; goats; lactation; lactic acid; monitoring; neutral detergent fiber; pH; quantitative polymerase chain reaction; rumen; rumen fermentation; ruminal acidosis; volatile fatty acids
Abstract:
... Six rumen-cannulated lactating Guanzhong goats were used to investigate changes in ruminal fermentation pattern and the microbiota following a subacute ruminal acidosis (SARA) inducing procedure. Induction of SARA was performed by increasing dietary non-fiber carbohydrate (NFC) to neutral detergent fiber (NDF) ratio from 1.02 (stage 1) to 1.24 (stage 2), 1.63 (stage 3) and 2.58 (stage 4). A dynami ...
DOI:
10.1016/S2095-3119(13)60231-2

91. Fermentation of alfalfa wet-fractionation liquids to volatile fatty acids by Streptococcus bovis and Megasphaera elsdenii

Author:
P.J. Weimer; M.F. Digman
Source:
Bioresource technology 2013 v.142 pp. 88-94
ISSN:
0960-8524
Subject:
Megasphaera elsdenii; Streptococcus bovis; alfalfa; coculture; fermentation; juices; lactic acid bacteria; leaves; nutrients; pH; proteins; temperature; volatile fatty acids
Abstract:
... “Green juice”, obtained by squeezing fresh alfalfa leaves inoculated with lactic acid bacteria, was fermented at room temperature for 7–21d to obtain 12–47glactic acidL−1. Inoculation of green juice with Streptococcus bovis and incubation at 39°C reduced fermentation time to 8–12h. The resulting “brown juice” from either fermentation had a pH of ∼4.5 and a protein precipitate. Upon adjustment to p ...
DOI:
10.1016/j.biortech.2013.05.016
PubMed:
23732922
CHORUS:
10.1016/j.biortech.2013.05.016

92. In Situ Biphasic Extractive Fermentation for Hexanoic Acid Production from Sucrose by Megasphaera elsdenii NCIMB 702410

Author:
Kieun Choi; Byoung Seung Jeon; Byung-Chun Kim; Min-Kyu Oh; Youngsoon Um; Byoung-In Sang
Source:
Applied biochemistry and biotechnology 2013 v.171 no.5 pp. 1094-1107
ISSN:
0273-2289
Subject:
Megasphaera elsdenii; alcohols; bacteria; carbon; cell growth; direct contact; economics; fermentation; hexanoic acid; sodium; sucrose
Abstract:
... Hexanoic acid production by a bacterium using sucrose as an economic carbon source was studied under conditions in which hexanoic acid was continuously extracted by liquid–liquid extraction. Megasphaera elsdenii NCIMB 702410, selected from five M. elsdenii strains, produced 4.69 g l⁻¹hexanoic acid in a basal medium containing sucrose. Production increased to 8.19 g l⁻¹when the medium was supplemen ...
DOI:
10.1007/s12010-013-0310-3
PubMed:
23754557

93. One-Pot Microbial Production, Mechanical Properties, and Enzymatic Degradation of Isotactic P[(R)-2-hydroxybutyrate] and Its Copolymer with (R)-Lactate

Author:
Matsumoto Ken’ichiro; Terai Satsuki; Ishiyama Ayako; Sun Jian; Kabe Taizo; Song Yuyang; Nduko John Masani; Iwata Tadahisa; Taguchi Seiichi
Source:
Biomacromolecules 2013 v.14 no.6 pp. 1913-1918
ISSN:
1526-4602
Subject:
Escherichia coli; Megasphaera elsdenii; Pseudomonas; carboxylic ester hydrolases; composite polymers; condensation reactions; culture flasks; economic costs; enzyme activity; films (materials); glucose; mechanical properties; molecular weight; polyhydroxyalkanoates; polylactic acid; protein synthesis; proteinases; synthesis
Abstract:
... P[(R)-2-hydroxybutyrate] [P((R)-2HB)] is an aliphatic polyester analogous to poly(lactic acid) (PLA). However, little has been known for its properties because of a high cost of commercially available chiral 2HB as a starting substance for chemical polymer synthesis. In this study, P[(R)-2HB] and P[(R)-2HB-co-(R)-lactate] [P((R)-2HB-co-(R)-LA)] with a new monomer combination were successfully synt ...
DOI:
10.1021/bm400278j

94. Quantitative analysis of growth and volatile fatty acid production by the anaerobic ruminal bacterium Megasphaera elsdenii T81

Author:
P. J. Weimer; G. N. Moen
Source:
Applied microbiology and biotechnology 2013 v.97 no.9 pp. 4075-4081
ISSN:
0175-7598
Subject:
Megasphaera elsdenii; acetates; anaerobic digestion; bacteria; butyrates; carbohydrate metabolism; culture media; dose response; fermentation; glucose; glycogen; hexanoic acid; lactates; lipid metabolism; microbial growth; pH; propionates; quantitative analysis; rumen bacteria; valeric acid; volatile fatty acids
Abstract:
... Megasphaera elsdenii T81 grew on either DL-lactate or D-glucose at similar rates (0.85 h⁻¹) but displayed major differences in the fermentation of these substrates. Lactate was fermented at up to 210-mM concentration to yield acetic, propionic, butyric, and valeric acids. The bacterium was able to grow at much higher concentrations of D-glucose (500 mM), but never removed more than 80 mM of glucos ...
Handle:
10113/56869
DOI:
10.1007/s00253-012-4645-4
PubMed:
23271673

95. The Use of Quantitative Real Time Polymerase Chain Reaction to Quantify Some Rumen Bacterial Strains in an In Vitro Rumen System

Author:
Lucy Onime; Cristina Zanfi; Chiara Agostinis; Roberta Bulla; Mauro Spanghero
Source:
Italian journal of animal science 2013 v.12 no.3 pp. e58
ISSN:
1828-051X
Subject:
Butyrivibrio fibrisolvens; Megasphaera elsdenii; Ruminococcus albus; Streptococcus bovis; fermentation; forage; liquids; pH; rumen
Abstract:
... The aim of this work was to quantify four rumen bacterial strains (Butyrivibrio fibrisolvens, Ruminococcus albus, Streptococcus bovis, Megasphaera elsdenii) in an in vitro batch rumen fermentative system by quantitative real time polymerase chain reaction (qPCR). The experiment was a 2×2 factorial arrangement with two types of liquid rumen, collected from dairy cows (DC) and fattening bulls (FB) a ...
DOI:
10.4081/ijas.2013.e58

96. Changes in ruminal bacterial community composition following feeding of alfalfa ensiled with a lactic acid bacterial inoculant

Author:
R. Mohammed; D.M. Stevenson; K.A. Beauchemin; R.E. Muck; P.J. Weimer
Source:
Journal of dairy science 2012 v.95 no.1 pp. 328-339
ISSN:
0022-0302
Subject:
DNA; Holstein; Lactobacillus plantarum; Megasphaera elsdenii; acetates; alfalfa; alfalfa silage; bacterial communities; cannulas; community structure; corn; corn silage; correspondence analysis; cows; cross-over studies; crude protein; diet; digesta; dry matter intake; feed quality; genes; lipid content; milk; milk fat yield; minerals; neutral detergent fiber; pH; plate count; population size; propionic acid; quantitative polymerase chain reaction; ribosomal RNA; rumen; rumen bacteria; silage making; soybean hulls; vitamins; volatile fatty acids
Abstract:
... Some silage inoculants help to improve silage quality and promote an increase in milk production, possibly through altering the rumen microflora. We hypothesized that rumen bacterial community composition (BCC) would be different in cows fed alfalfa ensiled with the inoculant Lactobacillus plantarum MTD/1 (LP) compared with those fed alfalfa ensiled without the inoculant (Ctrl). Eight ruminally ca ...
DOI:
10.3168/jds.2011-4492
PubMed:
22192212
CHORUS:
10.3168/jds.2011-4492

97. Correlation between Composition of the Bacterial Community and Concentration of Volatile Fatty Acids in the Rumen during the Transition Period and Ketosis in Dairy Cows

Author:
Xiaoxu Wang; Xiaobing Li; Chenxu Zhao; Pan Hu; Hui Chen; Zhaoxi Liu; Guowen Liu; Zhe Wang
Source:
Applied and environmental microbiology 2012 v.78 no.7 pp. 2386-2392
ISSN:
0099-2240
Subject:
Lactobacillus; Megasphaera elsdenii; Selenomonas ruminantium; Streptococcus bovis; bacteria; body fat; community structure; dairy cows; energy balance; fatty acid composition; fatty liver; genes; glucose; ketosis; lactic acid; polymerase chain reaction; propionates; restriction fragment length polymorphism; ribosomal RNA; rumen; volatile fatty acids
Abstract:
... The transition period is a severe challenge to dairy cows. Glucose supply cannot meet demand and body fat is mobilized, potentially leading to negative energy balance (NEB), ketosis, or fatty liver. Propionate produces glucose by gluconeogenesis, which depends heavily on the number and species of microbes. In the present study, we analyzed the rumen microbiome composition of cows in the transition ...
DOI:
10.1128/AEM.07545-11
PubMed:
22267666
PubMed Central:
PMC3302620

98. Effects of a combination of hinokitiol (β‐thujaplicin) and an organic acid mixture on ruminal fermentation in heifers fed a high‐grain diet

Author:
Junichiro ISHII; Hiroshi OMURA; Tadao MITSUI; Norichika EGUCHI; Takashi UENO; Hisaya GOTO; Hiroshi ITO
Source:
Animal science journal = 2012 v.83 no.1 pp. 36-42
ISSN:
1344-3941
Subject:
Megasphaera elsdenii; Streptococcus bovis; Thujopsis dolabrata; alfalfa meal; antibacterial properties; bacteria; barley; citrates; diet; heifers; minimum inhibitory concentration; rumen fermentation; soybean meal; volatile fatty acids
Abstract:
... This study evaluated the effects of hinokitiol (a natural antibacterial compound extracted from Thujopsis dolabrata var. hondai) and an organic acid mixture (citrate content 50%) on ruminal fermentation. Antibacterial properties were examined by measuring minimal inhibitory concentration. Hinokitiol at 1.56 µg/mL or an organic acid mixture at 1600 µg/mL inhibited Streptococcus bovis growth. The co ...
DOI:
10.1111/j.1740-0929.2011.00915.x
PubMed:
22250737

99. Hydrogen fermentation using lactate as the sole carbon source: Solution for ‘blind spots’ in biofuel production

Author:
Akihiro Ohnishi; Yuji Hasegawa; Shinko Abe; Yukiko Bando; Naoshi Fujimoto; Masaharu Suzuki
Source:
RSC advances 2012 v.2 no.22 pp. 8332-8340
ISSN:
2046-2069
Subject:
Megasphaera elsdenii; carbon; fermenters; fuel production; glucose; heat stress; hydrogen; hydrogen production; inoculum; lactic acid; lactic acid bacteria; lactic fermentation; metabolites; methane; starch; xylose
Abstract:
... Lactate is not believed to be a suitable substrate for hydrogen fermentation, although glucose and starch are. To eliminate this ‘blind spot’ in hydrogen fermentation, we showed that it is feasible to produce hydrogen with lactate as the sole carbon source by using mixed microflora as well as a single culture. Microflora were isolated from a methane fermentation system, and their hydrogen producti ...
DOI:
10.1039/c2ra20590d

100. Inclusion of Chicory (Cichorium intybus L.) in Pigs' Diets Affects the Intestinal Microenvironment and the Gut Microbiota

Author:
Haoyu Liu; Emma Ivarsson; Johan Dicksved; Torbjörn Lundh; Jan Erik Lindberg
Source:
Applied and environmental microbiology 2012 v.78 no.12 pp. 4102-4109
ISSN:
0099-2240
Subject:
Cichorium intybus; Megasphaera elsdenii; Prevotella; acetic acid; bacterial communities; body weight; chicory; colon; community structure; correlation; diet; digesta; fermentation; forage; ileum; lactic acid bacteria; pH; plant fibers; restriction fragment length polymorphism; roots; swine; swine feeding
Abstract:
... The content and composition of prebiotic plant fiber in the diet is important in promoting gut-related health. This study investigated the effects of the dietary inclusion of chicory forage and roots on the intestinal microenvironment of pigs. Thirty-seven-week-old pigs were fed 1 of 5 diets for 18 days, including a cereal-based control diet and 4 diets with the inclusion of 80 and 160 g kg–1 of b ...
DOI:
10.1128/AEM.07702-11
PubMed:
22492453
PubMed Central:
PMC3370552