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1. Identification of active methanotrophs in a landfill cover soil through detection of expression of 16S rRNA and functional genes

Author:
Chen, Yin; Dumont, Marc G.; Cébron, Aurélie; Murrell, J. Colin
Source:
Environmental microbiology 2007 v.9 no.11 pp. 2855-2869
ISSN:
1462-2912
Subject:
Methylocella,   etc   ; Methylobacter; Methylocystis; Methylomonas; Methylosarcina; complementary DNA; denaturing gradient gel electrophoresis; gene expression; genes; landfills; messenger RNA; methane; methanol; methanotrophs; oxidation; reverse transcriptase polymerase chain reaction; ribosomal RNA; soil;     Show all 18 Subjects
Abstract:
... Active methanotrophs in a landfill soil were revealed by detecting the 16S rRNA of methanotrophs and the mRNA transcripts of key genes involved in methane oxidation. New 16S rRNA primers targeting type I and type II methanotrophs were designed and optimized for analysis by denaturing gradient gel electrophoresis. Direct extraction of RNA from soil enabled the analysis of the expression of the func ...
DOI:
10.1111/j.1462-2920.2007.01401.x
PubMed:
17922768