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1. 11'-α-Tocomonoenol is the major α-tocomonoenol isomer in cyanobacteria and microalgae from Costa Rica

Author:
Alexander Montoya-Arroyo; Katja Lehnert; Peter E. Lux; Víctor M. Jiménez; Patricia Esquivel; Ana Margarita Silva-Benavides; Walter Vetter; Jan Frank
Source:
Journal of food composition and analysis 2022 v.107 pp. 104325
ISSN:
0889-1575
Subject:
Arthrospira platensis; Chlorella vulgaris; Nannochloropsis; Tetraselmis; alpha-tocopherol; fatty acids; food composition; freeze drying; isomers; microalgae; photosynthesis; Costa Rica
Abstract:
... α-Tocomonoenols are vitamin E-derivatives with a single double-bond in the tocochromanol sidechain. Currently, two congeners are known: 11′-α-tocomonoenol and 12′-α-tocomonoenol, the later better known as “marine-derived tocopherol” (MDT). Information regarding their presence in photosynthetic aquatic organisms is scarce. We thus determined vitamin E congeners and derivatives in microalgae and cya ...
DOI:
10.1016/j.jfca.2021.104325

2. A CRISPR/dCas9-based transcription activated system developed in marine microalga Nannochloropsis oceanica

Author:
Li Wei; Zhouyi Jiang; Bingqing Liu
Source:
Aquaculture 2022 v.546 pp. 737064
ISSN:
0044-8486
Subject:
DNA; Nannochloropsis; RNA; RNA interference; aquaculture; carbon dioxide; feeds; gene overexpression; genes; methylation; methyltransferases; microalgae; photosynthesis; transactivators; transcription (genetics); transcriptional activation
Abstract:
... Nannochloropsis is one of the most important microalgae utilized as the feedstuff in aquaculture and is emerging model oleaginous microalgae. Recently, different genetic tools such as RNA interference, gene overexpression and gene editing have been developed in Nannochloropsis. However, the available genetic modification methods based on CRISPR technology are rather limited for modulating gene exp ...
DOI:
10.1016/j.aquaculture.2021.737064

3. A novel approach to build algal consortia for sustainable biomass production

Author:
Shovon Mandal; Alina A. Corcoran
Source:
Algal research 2022 v.65 pp. 102734
ISSN:
2211-9264
Subject:
Nannochloropsis; biofuels; biomass production; computer simulation; feedstocks; functional diversity; laboratory experimentation; lipid content; microalgae; phenotype; research; salinity; specific growth rate; temperature
Abstract:
... In the last decade, microalgae have reemerged as a feedstock for biofuels and a diverse suite of bioproducts. Yet, considerable challenges must be overcome before algal biofuels and bioproducts become technoeconomically viable. At present, single algal strains selected for particular phenotypic traits, such as maximum specific growth rate or lipid content, are commonly scaled for cultivation in op ...
DOI:
10.1016/j.algal.2022.102734

4. Accumulation of medium chain fatty acids in Nannochloropsis oceanica by heterologous expression of Cuphea palustris thioesterase FatB1

Author:
Christian Südfeld; Aamna Kiyani; Hortense Buckens; Michal Hubáček; René H. Wijffels; Maria J. Barbosa; Sarah D'Adamo
Source:
Algal research 2022 v.64 pp. 102665
ISSN:
2211-9264
Subject:
Cuphea; Elaeis guineensis; Nannochloropsis; biodiversity; deforestation; gene expression; health promotion; heterologous gene expression; lipid content; microalgae; oils; research; therapeutics
Abstract:
... Medium chain fatty acids (MCFAs) are compounds of considerable commercial interest that have applications in food, feed, and the production of industrially relevant chemicals. Due to their antipathogenic and health-promoting effects, they are further discussed as potential therapeutic agents for disease treatment and infection prevention and control. Domesticated agricultural crops that can grow i ...
DOI:
10.1016/j.algal.2022.102665

5. Antifungal properties of aqueous microalgal extracts

Author:
Benjamin Schmid; Luísa Coelho; Peter S.C. Schulze; Hugo Pereira; Tamára Santos; Inês B. Maia; Mário Reis; João Varela
Source:
Bioresource technology reports 2022 v.18 pp. 101096
ISSN:
2589-014X
Subject:
Alternaria alternata; Athelia rolfsii; Botrytis cinerea; Chlorella vulgaris; Nannochloropsis; Phaeodactylum tricornutum; Scenedesmus obliquus; Spirulina; Thanatephorus cucumeris; antifungal properties; food production; microalgae; mycelium; technology
Abstract:
... Microalgae are considered a promising source of eco-friendly antifungal agents that can potentially reduce the usage of synthetic fungicides. Hence, properties of aqueous extracts from Nannochloropsis sp., Phaeodactylum tricornutum, Scenedesmus obliquus, Chlorella vulgaris and Spirulina sp. were investigated in vitro regarding their antagonistic activity towards the phytopathogenic fungi Sclerotiu ...
DOI:
10.1016/j.biteb.2022.101096

6. Application of genetic disruption of a Nannochloropsis oceanica cell wall synthesizing gene to n-3 HUFA enrichment of Brachionus plicatilis

Author:
Hideaki Matsui; Shinji Sugihara; Mayumi Wada; Tatsurou Ozaki; Takeshi Saitoh; Tomonari Kotani
Source:
Aquaculture 2022 v.552 pp. 738022
ISSN:
0044-8486
Subject:
Brachionus plicatilis; Nannochloropsis; cell walls; cellulose synthase; diet; eicosapentaenoic acid; fish oils; genes; genetically modified organisms; homologous recombination; larviculture; live feeds; marine fish; microalgae; nutritive value; omega-3 fatty acids; phospholipids; ultrastructure
Abstract:
... It is believed that microalgae of the genus Nannochloropsis can replace fish oil as an n-3 highly unsaturated fatty acid (n-3 HUFA) source in live feed for sustainable marine fish larviculture. This is due to the fact that these microalgae are rich in eicosapentaenoic acid (EPA). On the other hand, the cell wall of microalgae is an obstacle to enriching live feed with n-3 HUFA. Manipulation of the ...
DOI:
10.1016/j.aquaculture.2022.738022

7. Bacterial diversity in different outdoor pilot plant photobioreactor types during production of the microalga Nannochloropsis sp. CCAP211/78

Author:
Jie Lian; Georg Steinert; Jeroen de Vree; Sven Meijer; Christa Heryanto; Rouke Bosma; René H. Wijffels; Maria J. Barbosa; Hauke Smidt; Detmer Sipkema
Source:
Applied microbiology and biotechnology 2022 v.106 no.5-6 pp. 2235-2248
ISSN:
0175-7598
Subject:
Bacteroidetes; Nannochloropsis; alpha-Proteobacteria; bacterial communities; community structure; correspondence analysis; delta-Proteobacteria; genes; inoculum; microalgae; nitrates; photobioreactors
Abstract:
... As large-scale outdoor production cannot be done in complete containment, cultures are (more) open for bacteria, which may affect the productivity and stability of the algae production process. We investigated the bacterial diversity in two indoor reactors and four pilot-scale outdoor reactors for the production of Nannochloropsis sp. CCAP211/78 spanning four months of operation from July to Octob ...
DOI:
10.1007/s00253-022-11815-3

8. Biodiesel production from Nannochloropsis oculata cultured at stressful carbon dioxide concentration and light illumination

Author:
Jun Zhang; Wen-Yi Tsai; Chao-Hung Hsu; Ching-An Peng
Source:
Biofuels 2022 v.13 no.4 pp. 527-535
ISSN:
1759-7277
Subject:
Nannochloropsis; biodiesel; carbon dioxide; cell growth; gas chromatography; light intensity; lighting; lipid content; lipids; microalgae; photobioreactors; transesterification
Abstract:
... Nannochloropsis oculata is a promising source of biodiesel due to the production of cellular lipid which can be converted to biodiesel through transesterification reaction. In this study, N. oculata cells were cultured under a medium light intensity and high CO₂ concentrations to improve cell growth and cellular lipid content. With an appropriate initial cell concentration, a 1.5-fold increase in ...
DOI:
10.1080/17597269.2020.1787699

9. Bioethanol production from defatted biomass of Nannochloropsis oculata microalgae grown under mixotrophic conditions

Author:
Nashwa A. H. Fetyan; Abo El-Khair B. El-Sayed; Fatma M. Ibrahim; Yasser A. Attia; Mahmoud W. Sadik
Source:
Environmental science and pollution research 2022 v.29 no.2 pp. 2588-2597
ISSN:
0944-1344
Subject:
Nannochloropsis; Saccharomyces cerevisiae; acid hydrolysis; agricultural wastes; biomass; carbon; carbon dioxide; economic feasibility; environmental sustainability; enzymatic hydrolysis; enzymatic treatment; ethanol; ethanol production; fermentation; glucose; hydrolysates; microalgae; nutrition; pollution; polysaccharides; research; sugarcane bagasse; wet digestion method
Abstract:
... In order to improve the economic feasibility and environmental sustainability of microalgal bioethanol production, a nontoxic, copious agricultural waste, sugarcane bagasse aqueous extract (SBAE) was used for cultivating Nannochloropsis oculata microalga (NNO-1 UTEX Culture LB 2164) as potential sources of substitutes for traditional nutrition to reduce the costs in cultivation through acid digest ...
DOI:
10.1007/s11356-021-15758-6

10. Circulating exosome miRNA, is it the novel nutrient molecule through cross-kingdom regulation mediated by food chain transmission from microalgae to bivalve?

Author:
Zhe Zheng; Zhijie Xu; Caixia Cai; Yongshan Liao; Chuangye Yang; Xiaodong Du; Ronglian Huang; Yuewen Deng
Source:
Comparative biochemistry and physiology 2022 pp. 101004
ISSN:
1744-117X
Subject:
Nannochloropsis; Pinctada fucata; apoptosis; biomineralization; endocytosis; energy metabolism; exosomes; food chain; gene expression; hemolymph; mammals; microRNA; microalgae; oysters; sequence analysis; signal transduction
Abstract:
... MicroRNAs (miRNAs) can efficiently regulate gene expression at intracellular and extracellular levels. Plant-derived miRNAs are highly enriched in animal haemolymph and regulate mammalian gene expression. However, evidence for food-derived miRNAs in Mollusca species is lacking. In this study, we fed the microalga Nannochloropsis oculata to the pearl oyster Pinctada fucata martensii and detected di ...
DOI:
10.1016/j.cbd.2022.101004

11. Comparative Life Cycle Assessment of a microalgae-based oil metal working fluid with its petroleum-based and vegetable-based counterparts

Author:
Mélanie Guiton; David Suárez-Montes; Rubén Sánchez; Paul Baustert; Christos Soukoulis; Burcu Saner Okan; Tommaso Serchi; Sébastien Cambier; Enrico Benetto
Source:
Journal of cleaner production 2022 v.338 pp. 130506
ISSN:
0959-6526
Subject:
Nannochloropsis; algae culture; bioeconomics; biofuels; biomass; climate change; coproducts; electric energy consumption; electricity; environmental impact; food production; inventories; microalgae; oils; petroleum; photobioreactors; rapeseed; vegetables
Abstract:
... Microalgae recently emerged as an alternative resource to terrestrial plant-based biomass for the bio-economy, limiting the competition with food production. Whereas its main field of application is into biofuels and bioenergy products, recent developments have shown the potential of microalgae biomass for product manufacturing, including Metal Working Fluids (MWF). The environmental impact of suc ...
DOI:
10.1016/j.jclepro.2022.130506

12. Computer-Aided Exergy Evaluation of Hydrothermal Liquefaction for Biocrude Production from Nannochloropsis sp.

Author:
Ziba Borazjani; Reza Azin; Shahriar Osfouri; Markus Lehner; Markus Ellersdorfer
Source:
BioEnergy research 2022 v.15 no.1 pp. 141-153
ISSN:
1939-1234
Subject:
Nannochloropsis; algae; biofuels; biomass; exergy; heat exchangers; heat transfer; hydrothermal liquefaction; research; temperature
Abstract:
... Biomass (especially algae) is a renewable energy source that can be a great alternative to fossil fuels. Wet algal biomass converts into products such as solid, aqueous, and gaseous phases as well as biocrude in hydrothermal liquefaction (HTL). The aim of this work was to provide detailed exergy analyses of the production of biocrude from Nannochloropsis sp. by HTL. Physical and chemical exergy of ...
DOI:
10.1007/s12155-021-10297-x

13. Cytotoxicity and 1H NMR metabolomics analyses of microalgal extracts for synergistic application with Tamoxifen on breast cancer cells with reduced toxicity against Vero cells

Author:
Hanaa Ali Hussein; Murni Nur Islamiah Kassim; M. Maulidiani; Faridah Abas; Mohd Azmuddin Abdullah
Source:
Heliyon 2022 v.8 no.3 pp. e09192
ISSN:
2405-8440
Subject:
Chlorella; Nannochloropsis; Tetraselmis suecica; alanine; apoptosis; biopharmaceuticals; breast neoplasms; caspases; chloroform; cytotoxicity; ethanol; freshwater; fumaric acid; healthy diet; hexane; lactic acid; linoleic acid; metabolomics; methanol; microalgae; oleic acid; principal component analysis; solvents; tamoxifen
Abstract:
... This study evaluated the cytotoxic activity of Tamoxifen (TMX), an anti-estrogen drug, with microalgal crude extracts (MCEs) in single and synergistic application (TMX-MCEs) on MCF-7 and 4T1 breast cancer cells, and non-cancerous Vero cells. The MCEs of Nannochloropsis oculata, Tetraselmis suecica and Chlorella sp. from five different solvents (methanol, MET; ethanol, ETH; water, W; chloroform, CH ...
DOI:
10.1016/j.heliyon.2022.e09192

14. Determination of intracellular lipid and main fatty acids of Nannochloropsis oceanica by ATR-FTIR spectroscopy

Author:
Dongmei Zhang; Qijun Li; Chenghu Yan; Wei Cong
Source:
Journal of applied phycology 2022 v.34 no.1 pp. 343-352
ISSN:
0921-8971
Subject:
Nannochloropsis; algology; biomass; carbohydrates; cooking fats and oils; eicosapentaenoic acid; fatty acid composition; least squares; light intensity; lipid content; microalgae; models; nitrogen content; palmitic acid; palmitoleic acid; prediction
Abstract:
... Fatty acid profile was one of the important characteristic parameters of edible oil. With the intensification of interest in microalgae as a potential source of edible oil, rapid methods are needed to determine the fatty acid composition of microalgae. Attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) was applied to determine the lipid and fatty acid contents of Nannoc ...
DOI:
10.1007/s10811-021-02607-9

15. Effect of adding microalgae to whiteleg shrimp culture on water quality, shrimp development and yield

Author:
Chen Huang; Yuanyuan Luo; Guoquan Zeng; Penglong Zhang; Ruibing Peng; Xiamin Jiang; Maowang Jiang
Source:
Aquaculture reports 2022 v.22 pp. 100916
ISSN:
2352-5134
Subject:
Litopenaeus vannamei; Nannochloropsis; Thalassiosira pseudonana; ammonium nitrogen; dissolved oxygen; feed conversion; growth performance; microalgae; nitrate nitrogen; nitrite nitrogen; pH; sediments; shrimp; shrimp culture; water quality; weight gain
Abstract:
... This study investigated the effects of microalgae addition on the water quality, accumulation of Vibrios in the water and sediment, and growth performance of whiteleg shrimp (Litopenaeus vannamei) in the intensive culture system. To evaluate the effects of two monospecific microalgae in whiteleg shrimp culture, an 84-day experiment was performed in concrete tanks seeded with Nannochloropsis oculat ...
DOI:
10.1016/j.aqrep.2021.100916

16. Effect of different feeds on reproduction performance, nutritional components and fatty acid composition of cladocer water flea (Daphnia magna)

Author:
Gülşah Turcihan; Melek Isinibilir; Yusuf Gufran Zeybek; Kamil Mert Eryalçın
Source:
Aquaculture research 2022 v.53 no.6 pp. 2420-2430
ISSN:
1355-557X
Subject:
Botryococcus braunii; Chlorella vulgaris; Daphnia magna; Nannochloropsis; adults; aquaculture; bakers yeast; biomass; diet; eggs; fatty acid composition; females; freshwater; herbivores; juveniles; lipids; microalgae; population density; reproductive performance; research; spray drying; zooplankton
Abstract:
... This research evaluated the growth, survival, proximate and fatty acid composition of water flea (Daphnia magna) fed baker's yeast (BY) and three types of microalgae diets. In this study, two freshwater microalgae; Botrycoccus braunii (BB) and Chlorella vulgaris (CV), one marine spray‐dried microalgae, Nannochloropsis oculata (SDN), and baker's yeast were evaluated for herbivorous zooplankton Daph ...
DOI:
10.1111/are.15759

17. Effects of LED lighting on Nannochloropsis oceanica grown in outdoor raceway ponds

Author:
M. Carneiro; I.B. Maia; P. Cunha; I. Guerra; T. Magina; T. Santos; P.S.C. Schulze; H. Pereira; F.X. Malcata; J. Navalho; J. Silva; A. Otero; J. Varela
Source:
Algal research 2022 v.64 pp. 102685
ISSN:
2211-9264
Subject:
Nannochloropsis; biomass; carbohydrates; chlorophyll; economic analysis; light intensity; lighting; metabolites; microalgae; photosynthesis; research; solar radiation
Abstract:
... Growth in most microalgal mass cultivation systems is light-limited, particularly in raceway ponds (RWP) where the light path is higher. Artificial lighting can be a promising solution to diminishing dark zones and enhance microalgal productivity. Therefore, our goal was to prevent the cell shift from photosynthesis to a respiration-only stage by resorting to LED illumination. Nannochloropsis ocea ...
DOI:
10.1016/j.algal.2022.102685

18. Effects of Structural and Compositional Changes of Nanochloropsis oceania after Enzyme Treatment on EPA-Rich Lipids Extraction

Author:
Kangyu Zhao; Meilan Zhang; Hua Tian; Fenfen Lei; Dongping He; Jingcheng Zheng; Liwei Zhang
Source:
Marine drugs 2022 v.20 no.3 pp. -
ISSN:
1660-3397
Subject:
Nannochloropsis; cellulose; crystal structure; eicosapentaenoic acid; endo-1,4-beta-glucanase; enzymatic treatment; ethanol; hemicellulose; laccase; microalgae; Pacific Ocean Islands
Abstract:
... Improved methods for the extraction of eicosapentaenoic acid (EPA), an essential and economically important polyunsaturated fatty acid, are urgently required. However, lipid extraction rates using food-grade solvents such as ethanol are usually low. To improve the ethanol-based extraction rate, and to elucidate the relevant mechanisms, we used cellulase and laccase to treat powdered Nannochloropsi ...
DOI:
10.3390/md20030160

19. Effects of alternative and sustainable ingredients, insect meal, microalgae and protein and lipid from tuna cooking water, on meagre (Argyrosomus regius) growth, food conversion and muscle and liver composition

Author:
A. Estévez; B. Blanco; L. Fernández; M. Ferreira; M. Soula
Source:
Aquaculture 2022 v.548 pp. 737549
ISSN:
0044-8486
Subject:
Acheta domesticus; Argyrosomus regius; Isochrysis galbana; Nannochloropsis; Rhodomonas; animal growth; aquaculture; aquaculture feeds; diet; digestibility; feed conversion; food conversion; growth performance; insect meal; liver; microalgae; muscle protein; muscles; nutritive value; oils; omega-3 fatty acids; omega-6 fatty acids; protein content; tuna
Abstract:
... This study aimed to evaluate the effects of alternative feed ingredients: Insect meal (DI) using non-defatted meal obtained from Acheta domesticus, Microalgae (DM) using a mix of four marine microalgae ((Nannochloropsis gaditana, Tisochrysis lutea, Rhodomonas lens, Isochrysis galbana), Protein and oil from water of tuna canning process (DP&L) and a Mixture (DMix) of the three ingredients, on the g ...
DOI:
10.1016/j.aquaculture.2021.737549

20. Effects of demineralization on the composition of microalgae pyrolysis volatiles in py-GC–MS

Author:
Qi Niu; Stef Ghysels; Nannan Wu; Diederik P.L. Rousseau; Jan Pieters; Wolter Prins; Frederik Ronsse
Source:
Energy conversion and management 2022 v.251 pp. 114979
ISSN:
0196-8904
Subject:
Nannochloropsis; Scenedesmus; administrative management; amides; cell walls; chemical structure; energy conversion; feedstocks; freshwater; furans; heterocyclic nitrogen compounds; hydrolysis; microalgae; nitriles; principal component analysis; pyrolysis; ultrasonic treatment
Abstract:
... This study compared the volatiles distribution in analytical scale pyrolysis (py-GC–MS) of Nannochloropsis gaditana (marine microalgae) and Scenedesmus almeriensis (freshwater microalgae) and their demineralized counterparts. The role of inorganic elements and their removal via ultrasonic treatment, water washing and (in)organic acid leaching were elucidated. Principal component analysis (PCA) and ...
DOI:
10.1016/j.enconman.2021.114979

21. Effects of outdoor and indoor cultivation on the polar lipid composition and antioxidant activity of Nannochloropsis oceanica and Nannochloropsis limnetica: A lipidomics perspective

Author:
Daniela Couto; Tiago A. Conde; Tânia Melo; Bruna Neves; Margarida Costa; Pedro Cunha; Inês Guerra; Nádia Correia; Joana T. Silva; Hugo Pereira; João Varela; Joana Silva; Rosário Domingues; Pedro Domingues
Source:
Algal research 2022 v.64 pp. 102718
ISSN:
2211-9264
Subject:
Nannochloropsis; antioxidant activity; betaine; biomarkers; biomass; dietary supplements; eicosapentaenoic acid; freshwater; gas chromatography-mass spectrometry; glycolipids; lipidomics; liquid chromatography; microalgae; oleic acid; phospholipids; photobioreactors; research; temperature; value added
Abstract:
... Nannochloropsis is a genus of eicosapentaenoic acid-rich microalgae with high levels of value-added polar lipids. However, the polar lipid composition of microalgal biomass is highly dependent on culture conditions (e.g., light or temperature), which are significantly different under indoor and outdoor culture conditions. In this study, we sought to investigate the plasticity of the polar lipid pr ...
DOI:
10.1016/j.algal.2022.102718

22. Elucidating the ameliorative effects of the cyanobacterium Spirulina (Arthrospira platensis) and several microalgal species against the negative impacts of contaminants in freshwater fish: A review

Author:
Hany M.R. Abdel-Latif; Saeed El-Ashram; Alaa El-Din H. Sayed; Mahmoud Alagawany; Mustafa Shukry; Mahmoud A.O. Dawood; Dariusz Kucharczyk
Source:
Aquaculture 2022 v.554 pp. 738155
ISSN:
0044-8486
Subject:
Amphora; Arthrospira platensis; Chlorella pyrenoidosa; Chlorella vulgaris; Dunaliella salina; Haematococcus pluvialis; Nannochloropsis; Spirulina; agricultural runoff; antioxidant activity; antioxidants; aquaculture; aquaculture industry; farmed fish; feed composition; fish health; freshwater fish; genotoxicity; growth retardation; immunity; industrialization; microalgae; mortality; oxidative stress; phytochemicals; toxic substances; water pollution
Abstract:
... Generally, fishes are continuously exposed to several aquatic pollutants originating from agricultural runoff, mining activities, discharges from industrialized regions, or other sources that throw their polluted water into the aquatic ecosystems. Fish exposure to pollutants such as heavy metals, pesticides, herbicides, and other toxic chemicals will negatively affect their health and welfare. The ...
DOI:
10.1016/j.aquaculture.2022.738155

23. Enriching table eggs with n-3 polyunsaturated fatty acids through dietary supplementation with the phototrophically grown green algae Nannochloropsis limnetica: effects of microalgae on nutrient retention, performance, egg characteristics and health parameters

Author:
A.J.W. Mens; M.M. van Krimpen; S.K. Kar; F.J. Guiscafre; L. Sijtsma
Source:
Poultry science 2022 v.101 no.6 pp. 101869
ISSN:
0032-5791
Subject:
Nannochloropsis; dietary supplements; docosahexaenoic acid; egg yolk; eggs; eicosapentaenoic acid; fatty acid composition; feed intake; haptoglobins; intestines; microalgae; morphometry; nutrient retention; omega-3 fatty acids; rapeseed; rapeseed meal
Abstract:
... The fatty acid content of microalgae, especially the high content of omega-3 fatty acids such as eicosapentaenoic acid (EPA, C20:5) and docosahexaenoic acid (DHA, C22:6), could enrich eggs when fed to laying hens. Moreover, the properties and bioactive components of omega-3 fatty acids could positively influence the health and production performance of laying hens. In this study, the effects of dr ...
DOI:
10.1016/j.psj.2022.101869

24. Evaluating the effects of agitation by shaking, stirring and air sparging on growth and accumulation of biochemical compounds in microalgae cells

Author:
Sheriff Olalekan Ajala; Matthew L. Alexander
Source:
Biofuels 2022 v.13 no.3 pp. 371-381
ISSN:
1759-7277
Subject:
Dunaliella salina; Nannochloropsis; Neochloris; Oocystis; aeration; agitation; air; biodiesel; biomass production; carbohydrates; economic feasibility; ethanol production; feeds; lipid content; lipids; microalgae; protein sources; salinity; temperature
Abstract:
... The economic feasibility of microalgae biomass production for biofuels is evaluated based on biomass concentration and productivity, and accumulation of pigments, lipid, carbohydrate, and protein. These parametric indicators are commonly promoted in microalgae cultures by manipulating the growth conditions, such as altering the growth nutrients, temperature, salinity, and mixing intensity. In view ...
DOI:
10.1080/17597269.2020.1714161

25. Evaluation of Nannochloropsis gaditana raw and hydrolysed biomass at low inclusion level as dietary functional additive for gilthead seabream (Sparus aurata) juveniles

Author:
María Isabel Sáez; Alba Galafat; Antonio Jesús Vizcaíno; Elena Chaves-Pozo; María Dolores Ayala; Marta Arizcun; Francisco Javier Alarcón; María Dolores Suárez; Tomás Francisco Martínez
Source:
Aquaculture 2022 v.556 pp. 738288
ISSN:
0044-8486
Subject:
Nannochloropsis; Sparus aurata; animal growth; aquaculture; bioavailability; biomass; body composition; body weight; cellulose; color; digestive enzymes; digestive physiology; endo-1,4-beta-glucanase; enzymatic hydrolysis; fatty acids; fish; hydrolysis; intestinal mucosa; juveniles; lipid peroxidation; liver; microalgae; muscles; nutritional intervention; phenolic compounds; proximate composition
Abstract:
... Abundant research is being carried out in the last years aimed at exploring microalgal biomass as nutrient source for different species of aquacultured fish. Some microalgae species, such as Nannochloropsis gaditana, have thick cell walls rich in cellulose, which might well reduce the bioavailability of intracellular active compounds. Among the alternatives aimed at overcoming this limitation, cel ...
DOI:
10.1016/j.aquaculture.2022.738288

26. Experimental and modeling assessment of large-scale cultivation of microalgae Nannochloropsis sp. PTCC 6016 to reach high efficiency lipid extraction

Author:
N. Hajinajaf; Y. Rabbani; A. Mehrabadi; O. Tavakoli
Source:
International journal of environmental science and technology 2022 v.19 no.6 pp. 5511-5528
ISSN:
1735-1472
Subject:
Nannochloropsis; biodiesel; biomass; carbon dioxide; cell walls; climate change; cost effectiveness; energy; feedstocks; fuel production; greenhouse gases; lipids; microalgae; neural networks; prediction; regression analysis; solvents; ultrasonic treatment; wastewater; Persian Gulf
Abstract:
... Microalgae have been highlighted as one of the promising feedstocks for renewable fuel production owing to their high growth rate, ability to grow on wastewater, and to convert CO₂ into lipids, the main source of biodiesel. However, lipid extraction from microalgal cells is cumbersome due to its thick cell wall which precludes commercial biodiesel production from microalgae, and to improve the lip ...
DOI:
10.1007/s13762-021-03760-x

27. Growth, productivity and proximate profiling of indigenous marine microalgae from southern coast of Bangladesh as potential feedstuff for animal feed

Author:
Zahidul Islam; Helena Khatoon; Mohammad Redwanur Rahman; Sanzib Kumar Barman; Shahadat Hossain; Sabiha Zaman Usha; Shanur Jahedul Hasan; Jakia Hasan
Source:
Bioresource technology reports 2022 v.18 pp. 101025
ISSN:
2589-014X
Subject:
Chaetoceros; Chlorella; Nannochloropsis; Tetraselmis; animals; biomass production; carbohydrates; coasts; feeds; microalgae; nutrient content; protein composition; proximate composition; Bangladesh
Abstract:
... In this study, purely isolated marine microalgae from southern coast of Bangladesh were cultured for animal feedstuff. A total of four species were isolated from different coastal aqua-farms. Among the four identified species three species were green microalgae (Chlorella sp., Nannochloropsis sp., and Tetraselmis sp.) and one was brown microalgae (Chaetoceros sp.). Growth curves, productivity and ...
DOI:
10.1016/j.biteb.2022.101025

28. Hydrothermal co-liquefaction of rice straw and Nannochloropsis: The interaction effect on mechanism, product distribution and composition

Author:
Jia Xia; Long Han; Chengkun Zhang; Hui Guo; Nai Rong; Humair Ahmed Baloch; Pingjiang Wu; Guoqiang Xu; Kaili Ma
Source:
Journal of analytical and applied pyrolysis 2022 v.161 pp. 105368
ISSN:
0165-2370
Subject:
Nannochloropsis; catalysts; liquefaction; pyrolysis; raw materials; rice straw; solvents; synergism; temperature
Abstract:
... Catalytic co-liquefaction of rice straw and Nannochloropsis has been investigated in this study, using alkali catalyst (K₂CO₃) in glycerol-water solvent at different temperature, residence time and raw material mass ratios. The distribution of co-liquefaction products was examined in relation to the main reaction conditions. The synergistic effect of rice straw and Nannochloropsis co-liquefaction ...
DOI:
10.1016/j.jaap.2021.105368

29. Identification and expression analysis of genome-wide long noncoding RNA responsive CO2 fluctuated environment in marine microalga Nannochloropsis oceanica

Author:
Li Wei; Bingqing Liu; Danmei Liu; Zhengru Xu; Ruiping Wang; Wenfei Zhang
Source:
Marine pollution bulletin 2022 v.176 pp. 113419
ISSN:
0025-326X
Subject:
Nannochloropsis; carbon dioxide; cellulose; epigenetics; gene expression; genes; growth and development; marine pollution; microalgae; non-coding RNA; plant growth; protein synthesis; transcription (genetics)
Abstract:
... Long non-coding RNAs (lncRNAs) have been demonstrated to participate in plant growth and development as well as response to different biotic and abiotic stresses. However, the knowledge of lncRNA was limited in microalgae. In this study, by RNA deep sequencing, 134 lncRNAs were identified in marine Nannochloropsis oceanica in response to carbon dioxide fluctuation. Among them, there were 51 lncRNA ...
DOI:
10.1016/j.marpolbul.2022.113419

30. Improving the Nutritional, Structural, and Sensory Properties of Gluten-Free Bread with Different Species of Microalgae

Author:
Muhammad Waqas Qazi; Inês Gonçalves de Sousa; Maria Cristiana Nunes; Anabela Raymundo
Source:
Foods 2022 v.11 no.3 pp. -
ISSN:
2304-8158
Subject:
Chlorella vulgaris; Nannochloropsis; Tetraselmis chuii; air; antioxidant activity; biomass; dough; ethanol; gluten-free bread; microalgae; mineral content; nutritive value; odors; rheology
Abstract:
... Microalgae are an enormous source of nutrients that can be utilized to enrich common food of inherently low nutritional value, such as gluten-free (GF) bread. Addition of the algae species: Tetraselmis chuii (Tc), Chlorella vulgaris (Cv), and Nannochloropsis gaditana (Ng) biomass led to a significant increase in proteins, lipids, minerals (Ca, Mg, K, P, S, Fe, Cu, Zn, Mn), and antioxidant activity ...
DOI:
10.3390/foods11030397

31. Influence of waste plastic on pyrolysis of low-lipid microalgae: A study on thermokinetics, behaviors, evolved gas characteristics, and products distribution

Author:
Akash Kumar; Beibei Yan; Junyu Tao; Jian Li; Lata Kumari; Belay Tafa Oba; Moses Akintayo Aborisade; Guanyi Chen
Source:
Renewable energy 2022 v.185 pp. 416-430
ISSN:
0960-1481
Subject:
Nannochloropsis; activation energy; microalgae; plastics; polystyrenes; pyrolysis; renewable energy sources; synergism
Abstract:
... In this study, co-pyrolysis of low-lipid Nannochloropsis sp. (NS) and waste polystyrene (PS) was deeply investigated using TG-MS-FTIR and Fixed bed reactor to characterize their thermal behaviors, kinetics, evolved gases, and products distribution. The (D)TG investigation of the co-pyrolysis process revealed two distinct stages of thermal decomposition: the first stage (150–420 °C) was mostly caus ...
DOI:
10.1016/j.renene.2021.12.087

32. Inhibitory effect of marine microalgae used in shrimp hatcheries on Vibrio parahaemolyticus responsible for acute hepatopancreatic necrosis disease

Author:
Sonia Araceli Soto‐Rodriguez; Paola Magallón‐Servín; Melissa López‐Vela; Mario Nieves Soto
Source:
Aquaculture research 2022 v.53 no.4 pp. 1337-1347
ISSN:
1355-557X
Subject:
Chaetoceros calcitrans; Nannochloropsis; Tetraselmis suecica; Thalassiosira; Vibrio parahaemolyticus; antibacterial properties; antibiotics; aquaculture; avirulent strains; bacterial growth; carbohydrate metabolism; cell growth; coculture; hydrophilicity; microalgae; necrotizing hepatopancreatitis; research; seawater; shrimp; virulence; virulent strains
Abstract:
... Marine microalgae are potential producers of antimicrobial compounds. Monospecific, vibrio‐free co‐cultures of marine microalgae, Chaetoceros calcitrans, Tetraselmis suecica, Nannochloropsis sp. and Thalassiosira weissflogii, at the exponential phase of growth were inoculated with two Vibrio parahaemolyticus (Vp) strains. Vp M0904, a highly virulent strain responsible for acute hepatopancreatic ne ...
DOI:
10.1111/are.15668

33. Kinetic Modeling of Cd(II) and Pb(II) Biosorption from Aqueous Solution by Inactive Biomass of Nannochloropsis oculata Microalgae

Author:
Omar Nateras-Ramírez; Jaime López-Cervantes; Dalia I. Sánchez-Machado; Rocio J. Aguilar-Ruiz; María R. Martínez-Macias
Source:
Water, air, and soil pollution 2022 v.233 no.6 pp. 184
ISSN:
0049-6979
Subject:
Nannochloropsis; adsorption; air; aqueous solutions; biomass; biosorption; heavy metals; kinetics; microalgae; pH; soil pollution; water pollution
Abstract:
... The development of heavy metal treatment technologies plays a crucial role in avoiding contamination of water bodies. Nannochloropsis oculata biomass was used for Pb²⁺ and Cd²⁺ biosorption. The biosorption capacity (g metal × g biomass⁻¹) was determined by evaluating the effects of pH and biomass amount. The maximum biosorption capacities for Pb²⁺ and Cd²⁺ were 1087.20 ± 9.12 mg g⁻¹ and 934.44 ± 1 ...
DOI:
10.1007/s11270-022-05636-3

34. Larval cultivation of Periglypta chemnitzii based on microalgal dietary selection

Author:
Zhenghua Deng; Yuwei Zhang; Haijun Wei; Wang Zhao; Mingqiang Chen; Xingmei Huang; Youning Li; Gang Yu; Yu Wang
Source:
Aquaculture 2022 v.559 pp. 738444
ISSN:
0044-8486
Subject:
Bivalvia; Chaetoceros muelleri; Chlorella vulgaris; Dunaliella salina; Isochrysis galbana; Nannochloropsis; Pavlova; aquaculture; clams; diet; digestion; embryogenesis; ingestion; larvae; larval development; light microscopes; microalgae; plankton; progeny; shellfish; survival rate
Abstract:
... Periglypta chemnitzii is an edible shellfish whose hardy nature makes it an ideal species for cultivation by aquaculture. The crucial factor determining whether a species is appropriate for aquaculture is successful breeding of the offspring. In this study, we gathered information regarding reproduction and larval cultivation of P. chemnitzii and assessed the suitability of different microalgae di ...
DOI:
10.1016/j.aquaculture.2022.738444

35. Levels of taurine, hypotaurine and homotaurine, and amino acids profiles in selected commercial seaweeds, microalgae, and algae-enriched food products

Author:
Carlos Terriente-Palacios; Massimo Castellari
Source:
Food chemistry 2022 v.368 pp. 130770
ISSN:
0308-8146
Subject:
Chlorella vulgaris; Gracilaria; Nannochloropsis; Palmaria palmata; Porphyra; Ulva lactuca; Undaria pinnatifida; animals; chromatography; food chemistry; macroalgae; markets; microalgae; pasta; protein content; sulfonic acids; taurine
Abstract:
... Amino acids and sulfonic acid derivatives (Taurine-Tau; Hypotaurine-HypTau; Homotaurine-HTau) of 26 different species of commercial macroalgae, microalgae and 10 algae-enriched food products from the market were quantified in a single chromatographic run. Tau and analogues were predominantly distributed in red species followed by green and brown species. Palmaria palmata, Gracilaria longissima and ...
DOI:
10.1016/j.foodchem.2021.130770

36. Long‐term incorporation of Selenium and Zinc in microalgae Isochrysis galbana and Nannochloropsis oculata and its effects on rotifer

Author:
Nasrollah Ahmadifard; Sirwe Ghaderpour; Naser Agh; Zakaria Vahabzadeh; Alicia Estevez
Source:
Aquaculture research 2022 v.53 no.8 pp. 3188-3201
ISSN:
1355-557X
Subject:
Copepoda; Isochrysis galbana; Nannochloropsis; Rotifera; aquaculture; cell division; color; eggs; microalgae; population density; research; selenium; specific growth rate; zinc
Abstract:
... Rotifers are widely used in hatcheries to feed small‐sized aquatic larvae although one of their disadvantages is the lack of zinc and selenium 5‐ and 30‐fold lower than in copepods, respectively. To improve the rotifers quality, different concentrations of zinc and selenium (2, 4, 5 and 10 mg L⁻¹ of each mineral) were added to the medium of the microalgae Isochrysis aff. galbana and Nannochloropsi ...
DOI:
10.1111/are.15831

37. Manipulation of triacylglycerol biosynthesis in Nannochloropsis oceanica by overexpressing an Arabidopsis thaliana diacylglycerol acyltransferase gene

Author:
Lin Zhang; Song Wang; Ji-Chang Han; Guan-Pin Yang; Ke-Hou Pan; Ji-Lin Xu
Source:
Algal research 2022 v.61 pp. 102590
ISSN:
2211-9264
Subject:
Arabidopsis thaliana; Nannochloropsis; biofuels; biosynthesis; commercialization; diacylglycerol acyltransferase; electroporation; feedstocks; fossil fuels; gas chromatography-mass spectrometry; genes; genetic engineering; heat stress; microalgae; research; triacylglycerols
Abstract:
... Given the depleting fossil fuel resource, oleaginous microalgae have a great potential as feedstocks for sustainable and environment-friendly biofuels. Improvement of neutral lipid, especially triacylglycerol (TAG), accumulation, without a cost of growth is considerably desirable for the commercial feasibility of microalgae-derived biofuels. Genetic engineering emerges as a feasible and effective ...
DOI:
10.1016/j.algal.2021.102590

38. Metabolic engineering of the oleaginous alga Nannochloropsis for enriching eicosapentaenoic acid in triacylglycerol by combined pulling and pushing strategies

Author:
Jin Liu; Meijing Liu; Yufang Pan; Ying Shi; Hanhua Hu
Source:
Metabolic engineering 2022 v.69 pp. 163-174
ISSN:
1096-7176
Subject:
Nannochloropsis; algae; biosynthesis; chloroplasts; diacylglycerol acyltransferase; eicosapentaenoic acid; genes; photosynthesis; triacylglycerols
Abstract:
... The marine alga Nannochloropsis oceanica has been considered as a promising photosynthetic cell factory for synthesizing eicosapentaenoic acid (EPA), yet the accumulation of EPA in triacylglycerol (TAG) is restricted to an extreme low level. Poor channeling of EPA to TAG was observed in N. oceanica under TAG induction conditions, likely due to the weak activity of endogenous diacylglycerol acyltra ...
DOI:
10.1016/j.ymben.2021.11.015

39. Nannochloropsis oceanica biomass enriched by electrocoagulation harvesting with promising agricultural applications

Author:
Daniel Figueiredo; Alice Ferreira; Florinda Gama; Luisa Gouveia
Source:
Bioresource technology reports 2022 v.17 pp. 100979
ISSN:
2589-014X
Subject:
Nannochloropsis; Solanum lycopersicum; bioavailability; biomass; dewatering; electrocoagulation; electrodes; energy use and consumption; microalgae; models; nutrient content; tomatoes
Abstract:
... Electrocoagulation is a promising technology to harvest and concentrate microalgae while saving costs on secondary dewatering steps. However, the sacrificial electrodes release salts that impact the media and the harvested biomass. This study evaluated the effects of Fe, Zn, and Mg electrodes on Nannochloropsis oceanica harvesting and elementary composition of biomass and supernatants. Moreover, p ...
DOI:
10.1016/j.biteb.2022.100979

40. Nannochloropsis oceanica harvested using electrocoagulation with alternative electrodes – An innovative approach on potential biomass applications

Author:
Daniel Figueiredo; Alice Ferreira; Pedro Quelhas; Peter S.C. Schulze; Luisa Gouveia
Source:
Bioresource technology 2022 v.344 pp. 126222
ISSN:
0960-8524
Subject:
Nannochloropsis; aluminum; biomass; corrosion; electrocoagulation; electrodes; iron; magnesium; microalgae; pH; zinc
Abstract:
... Electrocoagulation is a promising technology to harvest microalgal biomass. However, the commonly used aluminum electrodes release undesired salts that decrease biomass value. In this study, alternative iron, zinc, and magnesium electrodes and operational parameters pH, time and current density were studied to harvest Nannochloropsis oceanica. For recovery efficiency and concentration factor the i ...
DOI:
10.1016/j.biortech.2021.126222

41. Oxidative torrefaction of microalga Nannochloropsis Oceanica activated by potassium carbonate for solid biofuel production

Author:
Congyu Zhang; Fanghua Li; Shih-Hsin Ho; Wei-Hsin Chen; Duleeka Sandamali Gunarathne; Pau Loke Show
Source:
Environmental research 2022 v.212 pp. 113389
ISSN:
0013-9351
Subject:
Nannochloropsis; biofuels; biomass; carbohydrates; feedstocks; fuel production; microalgae; potassium carbonate; research; torrefaction
Abstract:
... Oxidative torrefaction is a promising way for biomass upgrading and solid biofuel production. Alkali metals are considered to be efficient activators for enhancing biofuel upgrading during the thermal reaction process. Herein, the microalga Nannochloropsis Oceanica is selected as the feedstock for assessing potassium carbonate activated effect on solid biofuel production through oxidative torrefac ...
DOI:
10.1016/j.envres.2022.113389

42. Phenoplate: An innovative method for assessing interacting effects of temperature and light on non-photochemical quenching in microalgae under chemical stress

Author:
Andrei Herdean; Donna L. Sutherland; Peter J. Ralph
Source:
New biotechnology 2022 v.66 pp. 89-96
ISSN:
1871-6784
Subject:
Nannochloropsis; Tetraselmis; Thalassiosira pseudonana; biotechnology; microalgae; phenotype; phosphates; photobiology; photosynthesis; physiological state; radiation resistance; temperature
Abstract:
... Rapid light curves are one of the most widely used methods for assessing the physiological state of photosynthetic organisms. While the method has been applied in a range of physiological studies over the last 20 years, little progress has been made in adapting it for the new age of multi-parametric phenotyping. In order to advance research that is aimed at evaluating the physiological impact of m ...
DOI:
10.1016/j.nbt.2021.10.004

43. Potentiality of Nannochloropsis sp. as partial dietary replacement of fishmeal on growth, proximate composition, pigment and breeding performance in guppy (Poecilia reticulata)

Author:
Razia Sultana; Helena Khatoon; Mohammad Redwanur Rahman; Mohammad Ekramul Haque; Zannatul Nayma; Fardous Ara Mukta
Source:
Bioresource technology reports 2022 v.18 pp. 101112
ISSN:
2589-014X
Subject:
Nannochloropsis; Poecilia reticulata; average daily gain; carbohydrate content; carotenoids; comparative study; condition factor; feed conversion; fish; fish meal; lipid composition; muscles; progeny; proximate composition; reproductive performance; specific growth rate; technology
Abstract:
... In this study, fishmeal was partially replaced by Nannochloropsis sp. to evaluate its effect on growth, proximate composition, pigment and reproductive performance in guppy. Commercial feed and formulated diets with Nannochloropsis sp. incorporated at different levels were used for comparative study. Results showed that the lipid profile was significantly (P < 0.05) higher in 15 % inclusion of Nan ...
DOI:
10.1016/j.biteb.2022.101112

44. Promoter prediction in nannochloropsis based on densely connected convolutional neural networks

Author:
Pi-Jing Wei; Zhen-Zhen Pang; Lin-Jie Jiang; Da-Yu Tan; Yan-Sen Su; Chun-Hou Zheng
Source:
Methods 2022 v.204 pp. 38-46
ISSN:
1046-2023
Subject:
DNA; DNA-directed RNA polymerase; Nannochloropsis; data collection; microalgae; neural networks; prediction; synthetic biology; transcription (genetics); transcription initiation site
Abstract:
... Promoter is a key DNA element located near the transcription start site, which regulates gene transcription by binding RNA polymerase. Thus, the identification of promoters is an important research field in synthetic biology. Nannochloropsis is an important unicellular industrial oleaginous microalgae, and at present, some studies have identified some promoters with specific functions by biologica ...
DOI:
10.1016/j.ymeth.2022.03.017

45. Research on the thermochemical conversion utilization of nitrogen-rich microalgae: Two-step catalytic pyrolysis of Nannochloropsis sp over ZSM-5

Author:
Liqiang Zhang; Jiaojiao Liu; Dawei Li; Zhengda Yang; Xinwei Wang; Riyi Lin
Source:
Energy conversion and management 2022 v.258 pp. 115475
ISSN:
0196-8904
Subject:
Nannochloropsis; activation energy; administrative management; energy conversion; heterocyclic nitrogen compounds; kinetics; microalgae; models; nitriles; pyrolysis; pyrolysis gas chromatography; temperature; thermogravimetry; weight loss
Abstract:
... To explore an effective method of utilizing nitrogen-rich microalgae, the two-step pyrolysis (TSP) characteristic of Nannochloropsis sp (NCS) over ZSM-5 were studied by pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) and thermogravimetric analyzer (TG). The results showed that the TG process of NCS was classified into three stages. ZSM-5 increased the final weight loss and the pyrolysis ...
DOI:
10.1016/j.enconman.2022.115475

46. Seawater with Added Monosodium Glutamate Residue (MSGR) Is a Promising Medium for the Cultivation of Two Commercial Marine Microalgae

Author:
Siteng Yu; Ze Yu; Qingjie Hou; Haiyan Pei
Source:
Water 2022 v.14 no.6 pp. -
ISSN:
2073-4441
Subject:
Nannochloropsis; Phaeodactylum tricornutum; biobased products; biomass; culture media; lipids; metabolites; microalgae; monosodium glutamate; nitrogen; prices; seawater; wastewater
Abstract:
... Phaeodactylum tricornutum and Nannochloropsis oceanica, with their satisfactory performance in accumulating lipids and other high-value products, have been successfully used for commercial production in recent years. However, costly chemicals in culture media greatly increase the price of the resulting bioproducts. To control the cultivation cost, this paper assessed the potential of seawater supp ...
DOI:
10.3390/w14060975

47. Separation of microalgae using a compacted magnetite-containing gel bed

Author:
Takehiro Washino; Mikihide Demura; Shintaro Morisada; Keisuke Ohto; Hidetaka Kawakita
Source:
Bioprocess and biosystems engineering 2022 v.45 no.2 pp. 321-331
ISSN:
1615-7591
Subject:
Anabaena; Desmodesmus; Monoraphidium; Nannochloropsis; bioprocessing; differential equation; gels; microalgae
Abstract:
... Separation of microalgae of various sizes and shapes is an important process that enables subsequent production of useful compounds. Herein, the separation of microalgae was accomplished using a magnetite-containing gel (42 μm) packed into a column. An algal suspension was injected into the top of the gel bed, after which water was passed through the column. The pressure generated during the proce ...
DOI:
10.1007/s00449-021-02662-z

48. Supercritical fluid extraction and pressurized liquid extraction processes applied to eicosapentaenoic acid-rich polar lipid recovery from the microalga Nannochloropsis sp

Author:
María J. Jiménez Callejón; Alfonso Robles Medina; María D. Macías Sánchez; Pedro A. González Moreno; Elvira Navarro López; Luis Esteban Cerdán; Emilio Molina Grima
Source:
Algal research 2022 v.61 pp. 102586
ISSN:
2211-9264
Subject:
Nannochloropsis; atmospheric pressure; biomass; carbon dioxide; eicosapentaenoic acid; ethanol; fractionation; glycolipids; hexane; liquids; microalgae; phospholipids; research; solid phase extraction; supercritical fluid extraction
Abstract:
... This work studies the simultaneous extraction and fractionation of saponifiable lipids into neutral saponifiable lipids and polar lipids (phospholipids and glycolipids) from the microalga Nannochloropsis sp. (41.9% neutral saponifiable lipids, 58.1% polar lipids, 32.5% eicosapentaenoic acid, EPA) to obtain EPA-rich polar lipid fractions, using green techniques. Several two-step extraction processe ...
DOI:
10.1016/j.algal.2021.102586

49. Synergistic effect of ultrasound and switchable hydrophilicity solvent promotes microalgal cell disruption and lipid extraction for biodiesel production

Author:
Hao Guo; Jun Cheng; Yuxiang Mao; Lei Qian; Weijuan Yang; Ji-Yeon Park
Source:
Bioresource technology 2022 v.343 pp. 126087
ISSN:
0960-8524
Subject:
Nannochloropsis; biodiesel; cell membranes; cell walls; cellulose; electrostatic interactions; hydrogen bonding; hydrophilicity; microalgae; phospholipids; solvents; synergism; ultrasonic treatment; ultrasonics; zeta potential
Abstract:
... To facilitate the lipid extraction from Nannochloropsis oceanica with thick cell wall using switchable hydrophilicity solvent, ultrasound-assisted N, N, N', N'-tetraethyl-1,3-propanediamine (TEPDA) was used to effectively destruct the cell wall. TEPDA cations were adsorbed on the cells via electrostatic force and formed the electron-donor–acceptor (EDA) complex with the hydroxyl groups in cellulos ...
DOI:
10.1016/j.biortech.2021.126087

50. Techno-economic assessment of microalgae production, harvesting and drying for food, feed, cosmetics, and agriculture

Author:
Bárbara Vázquez-Romero; José Antonio Perales; Hugo Pereira; Maria Barbosa; Jesús Ruiz
Source:
Science of the total environment 2022 v.837 pp. 155742
ISSN:
0048-9697
Subject:
Nannochloropsis; Phaeodactylum tricornutum; algae culture; biomass; centrifugation; cosmetics; environment; freeze drying; live feeds; ultrafiltration; Portugal
Abstract:
... The objective of this techno-economic analysis is to define the costs for an industrial microalgae production process, comparing different operation strategies (Nannochloropsis oceanica cultivation during the whole year or cultivation of two species, where Phaeodactylum tricornutum and Tisochrysis lutea alternate), production scales (1 and 10 ha), harvesting technologies (centrifugation or ultrafi ...
DOI:
10.1016/j.scitotenv.2022.155742

51. The Effect of Trophic Modes on Biomass and Lipid Production of Five Microalgal Strains

Author:
Andonia Nicodemou; Michalis Kallis; Anastasia Agapiou; Androulla Markidou; Michalis Koutinas
Source:
Water 2022 v.14 no.2 pp. -
ISSN:
2073-4441
Subject:
Isochrysis galbana; Nannochloropsis; Scenedesmus obliquus; Tetraselmis suecica; biomass production; biorefining; glucose; linoleic acid; microalgae; nutrition; palmitic acid; water
Abstract:
... Five microalgae strains, namely Isochrysis galbana, Microchloropsis gaditana, Scenedesmus obliquus, Nannochloropsis oculata and Tetraselmis suecica, were selected as potential candidates for polyunsaturated fatty acids’ production, evaluating biomass productivity and their capacity to accumulate high lipid contents under different trophic modes. Microalgae strains were cultivated in the presence o ...
DOI:
10.3390/w14020240

52. The effect of cell disruption on the extraction of oil and protein from concentrated microalgae slurries

Author:
Ronald Halim; Ioannis Papachristou; George Q. Chen; Huining Deng; Wolfgang Frey; Clemens Posten; Aude Silve
Source:
Bioresource technology 2022 v.346 pp. 126597
ISSN:
0960-8524
Subject:
Nannochloropsis; biomass; mass transfer; microalgae; oils; osmotic stress; slurries; technology; water solubility
Abstract:
... Novel cell-disruption combinations (autolytic incubation and hypotonic osmotic shock combined with HPH or pH12) were used to investigate the fundamental mass transfer of lipids and proteins from Nannochloropsis slurries (140 mg biomass/g slurry). Since neutral lipids exist as cytosolic globules, their mass transfer was directly dependent on disintegration of cell walls. Complete recovery was obtai ...
DOI:
10.1016/j.biortech.2021.126597

53. The effectiveness of Arthrospira platensis and microalgae in relieving stressful conditions affecting finfish and shellfish species: An overview

Author:
Hany M.R. Abdel-Latif; Saeed El-Ashram; Sevdan Yilmaz; Mohammed A.E. Naiel; Zulhisyam Abdul Kari; Noor Khalidah Abdul Hamid; Mahmoud A.O. Dawood; Joanna Nowosad; Dariusz Kucharczyk
Source:
Aquaculture reports 2022 v.24 pp. 101135
ISSN:
2352-5134
Subject:
Arthrospira platensis; Chlorella vulgaris; Dunaliella salina; Haematococcus pluvialis; Lobosphaera incisa; Nannochloropsis; Tetraselmis; ammonia; antioxidant activity; antioxidants; aquaculture; disease resistance; farmed fish; food shortages; hypoxia; immunity; microalgae; nitrites; pH; phytochemicals; shellfish; shrimp
Abstract:
... Farmed fish and shrimp are continuously challenged by multiple stressors during their life stages, such as hypoxia, pH fluctuations, different salinities, high nitrite, un-ionized ammonia, injury during handling, inadequate nutrition, or food shortage, which can eventually adversely impact their health, welfare, and growth rates. Besides, these stressors can weaken production and decrease their re ...
DOI:
10.1016/j.aqrep.2022.101135

54. The nucleolus as a genomic safe harbor for strong gene expression in Nannochloropsis oceanica

Author:
Christian Südfeld; Ana Pozo-Rodríguez; Sara A. Manjavacas Díez; René H. Wijffels; Maria J. Barbosa; Sarah D’Adamo
Source:
Molecular plant 2022 v.15 no.2 pp. 340-353
ISSN:
1674-2052
Subject:
DNA-directed RNA polymerase; Nannochloropsis; biofuels; cell nucleolus; economic feasibility; feedstocks; gene expression; genomics; industrial applications; internal ribosome entry sites; microalgae; transcription (genetics)
Abstract:
... Microalgae are used in food and feed, and they are considered a potential feedstock for sustainably produced chemicals and biofuel. However, production of microalgal-derived chemicals is not yet economically feasible. Genetic engineering could bridge the gap to industrial application and facilitate the production of novel products from microalgae. Here, we report the discovery of a novel gene expr ...
DOI:
10.1016/j.molp.2021.11.003

55. Tocochromanol Profiles in Chlorella sorokiniana, Nannochloropsis limnetica and Tetraselmis suecica Confirm the Presence of 11′-α-Tocomonoenol in Cultured Microalgae Independently of Species and Origin

Author:
Alexander Montoya-Arroyo; Katja Lehnert; Alejandra Muñoz-González; Ulrike Schmid-Staiger; Walter Vetter; Jan Frank
Source:
Foods 2022 v.11 no.3 pp. -
ISSN:
2304-8158
Subject:
Chlorella sorokiniana; Nannochloropsis; Tetraselmis suecica; fluorescence; freeze drying; isomers; liquid chromatography; microalgae; photosynthesis; vitamin E
Abstract:
... 11′-α-Tocomonoenol (11′-αT1) is structurally related to vitamin E and has been quantified in the microalgae Tetraselmis sp. and Nannochloropsis oceanica. However, it is not known whether 11′-αT1 is present in other microalgae independent of species and origin. The aim of this study was to analyze the tocochromanol profiles of Chlorella sorokiniana, Nannochloropsis limnetica, and Tetraselmis suecic ...
DOI:
10.3390/foods11030396

56. Transcriptome analysis of lipid metabolism in response to cerium stress in the oleaginous microalga Nannochloropsis oculata

Author:
Di Wu; Yuyong Hou; Jie Cheng; Tong Han; Nahui Hao; Bingjie Zhang; Xiang Fan; Xiang Ji; Fangjian Chen; Donghui Gong; Lei Wang; Patrick McGinn; Lei Zhao; Shulin Chen
Source:
Science of the total environment 2022 v.838 pp. 156420
ISSN:
0048-9697
Subject:
Nannochloropsis; acetyl coenzyme A; acyl-CoA oxidase; biogenesis; biomass; carbon; carbon dioxide fixation; cerium; environment; glycerol kinase; glycolysis; lipid content; lipid metabolism; metabolites; microalgae; photosynthesis; pyruvic acid; ribosomes; transcriptome; transcriptomics; triacylglycerol lipase; triacylglycerols
Abstract:
... Nannochloropsis oculata can accumulate large amounts of lipids under rare earth element (REE) conditions. However, the lipid accumulation mechanism responsible for REE stress has not been elucidated. In this study, the effects of cerium (the most abundant REE) on the growth and lipid accumulation of N. oculata were investigated. The de novo transcriptome data of N. oculata under cerium conditions ...
DOI:
10.1016/j.scitotenv.2022.156420

57. Ultrasound-Assisted Extraction of Nannochloropsis oculata with Ethanol and Betaine: 1,2-Propanediol Eutectic Solvent for Antioxidant Pigment-Rich Extracts Retaining Nutritious the Residual Biomass

Author:
Maria D. Gkioni; Vasilis Andriopoulos; Eleni Koutra; Sophia Hatziantoniou; Michael Kornaros; Fotini N. Lamari
Source:
Antioxidants 2022 v.11 no.6 pp. -
ISSN:
2076-3921
Subject:
Nannochloropsis; antheraxanthin; antioxidants; aquaculture feeds; betaine; biomass; chlorophyll; colorimetry; cosmetics; encapsulation; ethanol; gas chromatography; humidity; maceration; microalgae; pH; solvents; ultrasonic treatment; violaxanthin; wastes
Abstract:
... The aim of this study was the development of an efficient “green” extraction method of Nannochloropsis oculata to produce antioxidant extracts and nutritious residual biomass. Twenty-one extraction methods were evaluated by measuring the reactivity with the Folin–Ciocalteu reagent: ultrasonication or maceration at different temperatures with different organic solvents, extraction at different pH v ...
DOI:
10.3390/antiox11061103

58. Using spray-dried and encapsulated Nannochloropsis oculata biomasses in white spread

Author:
Derya Genc Polat; Yasar Durmaz; Nevzat Konar; Haniyeh Rasouli Pirouzian; Omer Said Toker; Ibrahim Palabiyik; Murat Tasan
Source:
Journal of applied phycology 2022 v.34 no.1 pp. 375-383
ISSN:
0921-8971
Subject:
Nannochloropsis; algology; biomass; chlorophyll; color; encapsulation; ingredients; microalgae; sensory evaluation; shelf life; spray drying; water activity; white chocolate
Abstract:
... In this study, dried or encapsulated Nannochloropsis oculata microalgal biomass was used in spread samples (0.00–0.75 g (100 g)⁻¹ spread) as natural pigment source. Physicochemical properties, color stability, and rheological and sensory properties were investigated to assess the possibility of using microalgae biomass in white chocolate spread (WCS). The results showed that the chlorophyll-a amou ...
DOI:
10.1007/s10811-021-02669-9

59. Variables that intervene in the weight of rotifer biomass and fatty acids

Author:
Carla Patricia Aguilar Samanamud; Wilmer Gaspar; Gheraldine Inga; Lennin Flores; Pedro Sanchez; Hanna Hernandez Acevedo; Roberta Borda Soares; Alfredo Olivera Galvez
Source:
Journal of the World Aquaculture Society 2022 v.53 no.3 pp. 741-753
ISSN:
0893-8849
Subject:
Isochrysis galbana; Nannochloropsis; Rotifera; aquaculture; biomass; docosahexaenoic acid; eicosapentaenoic acid; fatty acid composition
Abstract:
... The biomass of rotifers is used as live food in aquaculture; their quality is determined by the content of the main polyunsaturated fatty acids and the weight it acquires over time. The objective of this work was to evaluate the variables involved in this process to achieve higher quality. Within the rotifer culture, the following evaluations were carried out nine times (0, 12, 24, 48, 72, 96, 120 ...
DOI:
10.1111/jwas.12848

60. Zeocin treatment significantly elevated transformation efficiency of Nannochloropsis oceanica

Author:
Zhongyi Zhang; Li Guo; Hang Liu; Xiaohui Pan; Lixian Liu; Yanan Zong; Guanpin Yang
Source:
Journal of applied phycology 2022 v.34 no.3 pp. 1587-1594
ISSN:
0921-8971
Subject:
DNA; Nannochloropsis; algology; genetic engineering; genetic transformation; hygromycin B; microalgae; resistance genes
Abstract:
... Genetic transformation is critical to microalgal genetic engineering which generally prefers a high transformation efficiency. Here, we describe a simple and efficient method of elevating the transformation efficiency of Nannochloropsis oceanica. Algal cells were treated ahead of transformation with zeocin at 0.2 μg mL⁻¹ for 48 h to cause random double-strand breaks (DSB) of DNA. Active hygromycin ...
DOI:
10.1007/s10811-022-02703-4

61. A Multi-Species Investigation of Sponges’ Filtering Activity towards Marine Microalgae

Author:
Despoina Varamogianni-Mamatsi; Thekla I. Anastasiou; Emmanouela Vernadou; Nikos Papandroulakis; Nicolas Kalogerakis; Thanos Dailianis; Manolis Mandalakis
Source:
Marine drugs 2021 v.20 no.1 pp. -
ISSN:
1660-3397
Subject:
Isochrysis; Nannochloropsis; Phaeodactylum tricornutum; Sarcotragus; adverse effects; environmental impact; eutrophication; fish culture; microalgae; organic matter; phytoplankton; seawater
Abstract:
... Chronic discharge of surplus organic matter is a typical side effect of fish aquaculture, occasionally leading to coastal eutrophication and excessive phytoplankton growth. Owing to their innate filter-feeding capacity, marine sponges could mitigate environmental impact under integrated multitrophic aquaculture (IMTA) scenarios. Herein, we investigated the clearance capacity of four ubiquitous Med ...
DOI:
10.3390/md20010024

62. A co-cultivation process of Nannochloropsis oculata and Tisochrysis lutea induces morpho-physiological and biochemical variations potentially useful for biotechnological purposes

Author:
Michele Maglie; Costanza Baldisserotto; Alessandra Guerrini; Alessandra Sabia; Lorenzo Ferroni; Simonetta Pancaldi
Source:
Journal of applied phycology 2021 v.33 no.5 pp. 2817-2832
ISSN:
0921-8971
Subject:
Haptophyta; Nannochloropsis; algae culture; algology; biomass production; carotenoids; coculture; cosmetics; energy; fatty acid composition; gas chromatography-mass spectrometry; microalgae; phylogeny; phytoremediation; polysaccharides
Abstract:
... The biotechnological potential of microalgae has gained considerable importance in many applied fields: biomass production for food and feed, cosmeceutical and pharmaceutical products, energy and phytoremediation. The driving force that inspires the progress in microalgae production is the need for new cultivation systems to obtain simultaneously the maximum yield, reduction of water and nutrients ...
DOI:
10.1007/s10811-021-02511-2

63. A photobioreactor using Nannochloropsis oculata marine microalgae for removal of polycyclic aromatic hydrocarbons and sorption of metals in produced water

Author:
Isadora Machado Marques; Adna Caroline Vale Oliveira; Olivia Maria Cordeiro de Oliveira; Emerson Andrade Sales; Ícaro Thiago Andrade Moreira
Source:
Chemosphere 2021 v.281 pp. 130775
ISSN:
0045-6535
Subject:
Nannochloropsis; bioaccumulation; biodegradation; biodiesel; biomass; bioplastics; cell growth; culture media; iron; microalgae; naphthalene; oils; petroleum; photobioreactors; saline water; sorption; value added; zinc
Abstract:
... The objective of the present work is to evaluate the potential of the removal of PAHs and metal sorption for the treatment of petroleum produced water using a photobioreactor system with Nannochloropsis oculata microalgae. A set of photobioreactors with different gradients of produced water concentration diluted in saline water was designed, establishing five gradients (v/v): 0, 25, 50, 75 and 100 ...
DOI:
10.1016/j.chemosphere.2021.130775

64. A validated thermal and biological model for predicting algal productivity in large scale outdoor cultivation systems

Author:
Jonah M. Greene; David Quiroz; Samuel Compton; Peter J. Lammers; Jason C. Quinn
Source:
Algal research 2021 v.54 pp. 102224
ISSN:
2211-9264
Subject:
Chlorella vulgaris; Desmodesmus; Nannochloropsis; algae; carbon dioxide fixation; feedstocks; geometry; growth models; meteorological data; model validation; photobioreactors; research; spatial data; spring; summer; temperature; winter
Abstract:
... Proper assessment of the sustainability of algal products requires an understanding of algal growth rate with regional and temporal resolution. This study developed a bulk growth model that utilizes geospatial data, reactor geometry inputs, and a maximum of six strain-specific parameters to predict algal productivity with temporal and regional resolution. The model requires local weather data to c ...
DOI:
10.1016/j.algal.2021.102224

65. Acclimation of photosynthesis and lipids biosynthesis to prolonged nitrogen and phosphorus limitation in Nannochloropsis gaditana

Author:
Nicolò Fattore; Alessandra Bellan; Luca Pedroletti; Nicola Vitulo; Tomas Morosinotto
Source:
Algal research 2021 v.58 pp. 102368
ISSN:
2211-9264
Subject:
Nannochloropsis; acclimation; biomass production; biosynthesis; carbon; microalgae; nitrogen; phosphorus; photosynthesis; research; seawater; triacylglycerols
Abstract:
... Microalgae represent a potential sustainable source of molecules and materials and the improvement of the knowledge of their metabolic regulation is essential to maximize their potential. Nutrients deprivation stimulates the accumulation of reserve lipids, triacylglycerols but also inhibits photosynthesis with a negative impact on biomass production and therefore overall productivity.In this work, ...
DOI:
10.1016/j.algal.2021.102368

66. Accurate method for rapid biomass quantification based on specific absorbance of microalgae species with biofuel importance

Author:
D.L. Ambriz‐Pérez; E.E. Orozco‐Guillen; N.D. Galán‐Hernández; K.D. Luna‐Avelar; A. Valdez‐Ortiz; D.U. Santos‐Ballardo
Source:
Letters in applied microbiology 2021 v.73 no.3 pp. 343-351
ISSN:
0266-8254
Subject:
Chaetoceros muelleri; Dunaliella tertiolecta; Nannochloropsis; absorbance; algae culture; automation; biofuels; biomass; cell growth; computer software; feedstocks; fuel production; logit analysis; microalgae; microbiology; operating costs; prediction; spectroscopy
Abstract:
... The development of microalgae culture technology has been an integral part to produce biomass feedstock to biofuel production. Due to this, numerous attempts have been made to improve some operational parameters of microalgae production. Despite this, specialized research in cell growth monitoring, considered as a fundamental parameter to achieve profitable applications of microalgae for biofuels ...
DOI:
10.1111/lam.13519

67. Advanced mass balance characterization and fractionation of algal biomass composition

Author:
Stefanie Van Wychen; Steven M. Rowland; Kaitlin C. Lesco; Peter V. Shanta; Tao Dong; Lieve M. L. Laurens
Source:
Journal of applied phycology 2021 v.33 no.5 pp. 2695-2708
ISSN:
0921-8971
Subject:
Monoraphidium; Nannochloropsis; Scenedesmus; algology; biomass production; feedstocks; fractionation; microalgae
Abstract:
... Opportunities associated with biomass production and bioproduct isolation from algae-derived feedstocks are plentiful and promising; however, there are challenges associated with realizing these applications. One of the most important, and often overlooked, challenges is the lack of availability of a strong foundation of compositional analysis methods validated on microalgal biomass. Currently, co ...
DOI:
10.1007/s10811-021-02508-x

68. Algal Lysis by Sagittula stellata for the Production of Intracellular Valuables

Author:
Meng Wang; Wen qiao Yuan; Shibao Chen; Lifu Wang; Shuwen Zhao; Shanshan Li
Source:
Applied biochemistry and biotechnology 2021 v.193 no.8 pp. 2516-2533
ISSN:
0273-2289
Subject:
Nannochloropsis; Sagittula stellata; algicides; bacteria; biotechnology; cell membranes; fluorescence; genes; heat shock proteins; lipid content; marine environment; microalgae; polyunsaturated fatty acids; predation; secretion
Abstract:
... The purpose of this study was to examine the efficacy of the algicidal bacterium Sagittula stellata on the cell lysis of Nannochloropsis oceanica, a microalga found in the marine environment, in order to extract intracellular valuables. Algicidal bacteria are capable of lysing algal cell walls while keeping lipids and proteins intact yet separated. We obtained these microbes from locations with co ...
DOI:
10.1007/s12010-021-03502-2

69. Anti-listerial activity of microalgal fatty acid methyl esters and their possible applications as chicken marinade

Author:
Jeyakumar Balakrishnan; Prakash Ganapathi; Suganya Kannan; Murugan Marudhamuthu; Kathiresan Shanmugam
Source:
International journal of food microbiology 2021 v.339 pp. 109027
ISSN:
0168-1605
Subject:
Caenorhabditis elegans; Chromulina; Listeria monocytogenes; Nannochloropsis; antibacterial properties; biofilm; cell membrane structures; chicken meat; chickens; cold storage; fluorescence microscopy; food microbiology; food safety; intestines; mechanism of action; membrane permeability; metabolism; microalgae; minimum inhibitory concentration; model food systems; propidium; refrigeration; therapeutics; transmission electron microscopy
Abstract:
... Fatty acid methyl esters (FAMEs) from marine microalgae have been reported to possess antimicrobial activities against several Gram positive and Gram negative bacteria, but a majority of them needs to be explored. The objective of this study was to investigate the antibacterial activity, mechanism of FAMEs from selected marine microalgae against Listeria monocytogenes, and to elucidate its efficac ...
DOI:
10.1016/j.ijfoodmicro.2020.109027

70. Application of enriched Cyclops abyssorum divergens with mixed algal diet compared to Artemia franciscana for improving larval growth and body composition of Dicentrarchus labrax

Author:
Heba S. El-Sayed; Amal Z. Ghonim; Gihan M. El-Khodary; Mohamed A. El-Sheikh; Hanan M. Khairy
Source:
Aquaculture reports 2021 v.20 pp. 100715
ISSN:
2352-5134
Subject:
Artemia franciscana; Chlorella; Dicentrarchus labrax; Isochrysis galbana; Nannochloropsis; Tetraselmis chuii; algae; aquaculture; bass; biomass; body composition; carcass composition; diet; histidine; isoleucine; larval development; linolenic acid; methionine; palmitic acid; survival rate
Abstract:
... Larval rearing techniques with suitable nursery feed should be prioritized for better survival and growth. We evaluate the potential use of Cyclops abyssorum divergens as a natural live food candidate instead of the costly traditional Artemia franciscana in the feeding strategy for Dicentrarchus labrax larvae. C. abyssorum divergens and A. franciscana were enriched with mixed valuable algae strain ...
DOI:
10.1016/j.aqrep.2021.100715

71. Basic oxygen furnace slag as a support material for the cultivation of indigenous marine microalgae

Author:
Chun-Yen Chen; Kai-Wei Zhuang; Yu-Han Chang; Dillirani Nagarajan; Chieh-Chen Huang; Jo-Shu Chang
Source:
Bioresource technology 2021 v.342 pp. 125968
ISSN:
0960-8524
Subject:
Chlorella sorokiniana; Nannochloropsis; Tetraselmis suecica; agitation; biomass production; furnaces; light intensity; microalgae; oxygen; slags; sodium acetate
Abstract:
... Attached cultivation of microalgae is a suitable strategy for attaining high biomass productivity with effortless harvesting. This study evaluates the feasibility of using Basic Oxygen Furnace Slag (BOFS) as a carrier for microalgae cultivation. Among the three indigenous microalgae (namely, Chlorella sorokiniana PTC13, Tetraselmis suecica SC5, and Nannochloropsis oceanica DG), which were examined ...
DOI:
10.1016/j.biortech.2021.125968

72. Biobased Solvents for Pressurized Liquid Extraction of Nannochloropsis gaditana Omega-3 Lipids

Author:
Cristina Blanco-Llamero; F. Javier Señoráns
Source:
Marine drugs 2021 v.19 no.2 pp. -
ISSN:
1660-3397
Subject:
Isochrysis galbana; Nannochloropsis; Tetraselmis chuii; biomass; ethanol; fatty acid composition; fractionation; glycolipids; liquids; microalgae; oils; solvents; ultrasonics
Abstract:
... To develop greener extraction alternatives for microalgae biomass, ultrasound assisted extraction (UAE) and pressurized liquid extraction (PLE) with different biobased solvents were investigated, demonstrating that both techniques are useful alternatives for algal lipid extraction. Specifically, Nannochloropsis gaditana lipids were extracted by UAE and PLE at different temperatures and extraction ...
DOI:
10.3390/md19020107

73. Biosafety evaluation of Nannochloropsis oculata and Schizochytrium sp. oils as novel human milk fat substitutes

Author:
Changyang Qiu; Yongjin He; Zicheng Huang; Weisang Qiu; Jian Huang; Mingzi Wang; Bilian Chen
Source:
Food & function 2021 v.12 no.7 pp. 2972-2984
ISSN:
2042-650X
Subject:
Nannochloropsis; Schizochytrium; behavior; biosafety; body weight; cell viability; chemistry; corn oil; cytotoxicity; diet study techniques; feedstocks; food consumption; hematology; human milk fat; infant formulas; microalgae; necropsy; rats
Abstract:
... The biosafety assessment of novel human milk fat substitutes (HMFs) from microalgae oils of Nannochloropsis oculata and Schizochytrium sp. was evaluated by testing the cytotoxic activity using IEC-6 cells, and by conducting a sub-chronic 28-day dietary study using Sprague–Dawley (SD) suckling rats in this study. The results of the cytotoxic activity of IEC-6 cells treated with HMFs showed no appar ...
DOI:
10.1039/d0fo03000g

74. CRISPR/Cas9 disruption of glucan synthase in Nannochloropsis gaditana attenuates accumulation of β-1,3-glucose oligomers

Author:
Brian W. Vogler; Amy Ashford; Matthew C. Posewitz
Source:
Algal research 2021 v.58 pp. 102385
ISSN:
2211-9264
Subject:
CRISPR-Cas systems; Nannochloropsis; algae; beta-glucans; biomass; fuel production; genes; glucose; nitrogen; oligosaccharides; research; starvation; triacylglycerols
Abstract:
... Nannochloropsis species have garnered significant interest for biofuel production due to their ability to accumulate high levels of triacylglycerols (TAGs), especially following nitrogen-starvation. However, the first response to nutrient starvation is the synthesis of chrysolaminarin, a soluble β-1,3-glucan with β-1,6-branching. We employ CRISPR/Cas9 to knock out two key enzymes responsible for t ...
DOI:
10.1016/j.algal.2021.102385

75. Cell wall and organelle modifications during nitrogen starvation in Nannochloropsis oceanica F&M-M24

Author:
Bianca Roncaglia; Alessio Papini; Graziella Chini Zittelli; Liliana Rodolfi; Mario R. Tredici
Source:
Journal of applied phycology 2021 v.33 no.4 pp. 2069-2080
ISSN:
0921-8971
Subject:
Nannochloropsis; algology; biomass; cell walls; lipid content; macroautophagy; mitochondria; nitrogen; nutrient availability; photosynthesis; starvation; thylakoids; ultrastructure
Abstract:
... Nannochloropsis oceanica F&M-M24 is able to increase its lipid content during nitrogen starvation to more than 50% of the total biomass. We investigated the ultrastructural changes and the variation in the content of main cell biomolecules that accompany the final phase of lipid accumulation. Nitrogen starvation induced a first phase of thylakoid disruption followed by chloroplast macroautophagy a ...
DOI:
10.1007/s10811-021-02416-0

76. Changes in biochemical composition and fatty acid accumulation of Nannochloropsis oculata in response to different iron concentrations

Author:
Soheila Sabzi; Mehdi Shamsaie Mehrgan; Houman Rajabi Islami; Seyed Pezhman Hosseini Shekarabi
Source:
Biofuels 2021 v.12 no.1 pp. 1-7
ISSN:
1759-7277
Subject:
Nannochloropsis; algae; biofuels; carotenoids; chemical composition; culture media; fatty acids; iron; photosynthesis; research; triacylglycerols
Abstract:
... In this research, standard growth medium of Nannochloropsis oculata was manipulated with 5-, 10-, 15- and 20-fold increased (15.8, 31.6, 47 and 63 mg/L) and decreased (0.63, 0.316, 0.21, and 0.15 mg/L) chelated FeCl₃. The standard medium (control group) contained 3.16 mg/L FeCl₃. The results revealed that the highest iron concentration (63 mg/L) partially restricted photosynthetic efficiency (0.67 ...
DOI:
10.1080/17597269.2018.1489672

77. Changes in the Development and Reproductive Output of Nitokra lacustris pacifica (Crustacea: Copepoda) Yeatman, 1983 Under Short and Long Term Exposure to Synthetic and Biodegradable Microbeads

Author:
Annisa Nursabrina Jaapar; Roswati Md Amin; Kesaven Bhubalan; Erqa Shazira Sohaimi
Source:
Journal of polymers and the environment 2021 v.29 no.12 pp. 4060-4072
ISSN:
1566-2543
Subject:
Copepoda; Nannochloropsis; adults; algae; biodegradability; bioplastics; chronic exposure; composite polymers; egg production; eggs; ingestion; marine environment; microbeads; microplastics; nauplii; polyhydroxyalkanoates; polystyrenes; reproductive performance
Abstract:
... Microplastics have become a significant problem in the marine environment where a wide range of marine organisms can ingest them. Biodegradable plastics or bioplastics, which are now being widely sourced as an alternative to conventional non-biodegradable plastics, are also known to breakdown into microparticles. This study aims to investigate the interaction between two types of microbeads toward ...
DOI:
10.1007/s10924-021-02165-8

78. Chemical composition and apparent digestibility of a panel of dried microalgae and cyanobacteria biomasses in rainbow trout (Oncorhynchus mykiss)

Author:
R. Cerri; A. Niccolai; G. Cardinaletti; F. Tulli; F. Mina; E. Daniso; T. Bongiorno; G. Chini Zittelli; N. Biondi; M.R. Tredici; E. Tibaldi
Source:
Aquaculture 2021 v.544 pp. 737075
ISSN:
0044-8486
Subject:
Arthrospira platensis; Chlorella sorokiniana; Nannochloropsis; Nostoc; Oncorhynchus mykiss; Phaeodactylum tricornutum; Porphyridium; Tetraselmis suecica; acid insoluble ash; amino acid composition; aquaculture; biodiversity; biomass; carnivores; cost effectiveness; crude protein; digestibility; digestive enzymes; energy; essential amino acids; feces; fish; industry; microalgae; nutritive value; organic matter; soybean meal
Abstract:
... Despite a growing interest in microalgae and cyanobacteria as potential sources of nutrients in aquafeeds, little information is presently available on their nutritive value for carnivorous fish species. The aim of this study was to evaluate chemical composition and nutrient digestibility of a panel of microalgae and cyanobacteria dried biomasses (MACB), using rainbow trout (Oncorhynchus mykiss W. ...
DOI:
10.1016/j.aquaculture.2021.737075

79. Chemical composition of selected marine microalgae, with emphasis on lipid and carbohydrate production for potential use as feed resources

Author:
Kjell Inge Reitan; Gunvor Øie; Håvard Jørgensen; Xinxin Wang
Source:
Journal of applied phycology 2021 v.33 no.6 pp. 3831-3842
ISSN:
0921-8971
Subject:
Bacillariophyceae; Chaetoceros calcitrans; Nannochloropsis; Pavlova lutheri; Skeletonema costatum; Tetraselmis; algology; carbon; docosahexaenoic acid; eicosapentaenoic acid; lipid content; microalgae; nitrogen; nitrogen content; specific growth rate
Abstract:
... Marine microalgae are a promising sustainable source of lipids, omega-3 fatty acids, and carbohydrates. Selected microalgae species belonging to the Bacillariophyceae, Haptophyceae, Eustigmatophyceae, and Prasinophyceae were characterised for cellular content of carbon and nitrogen, and for production yields of lipids, fatty acids, total carbohydrates, and β-glucans. Carbon and nitrogen content sh ...
DOI:
10.1007/s10811-021-02586-x

80. Chemical looping staged conversion of microalgae with calcium ferrite as oxygen carrier: Pyrolysis and gasification characteristics

Author:
Yongzhuo Liu; Tao Wang; Xintao Zhang; Xiude Hu; Tao Liu; Qingjie Guo
Source:
Journal of analytical and applied pyrolysis 2021 v.156 pp. 105129
ISSN:
0165-2370
Subject:
Nannochloropsis; aromatic compounds; biofuels; biomass; calcium; ferrimagnetic materials; gasification; liquids; microalgae; oxidation; oxygen; pyrolysis; steam; synthesis gas
Abstract:
... Aiming to take full advantage of biomass featuring high volatiles and high oxygen content, we propose two chemical looping staged conversion (CLSC) schemes by decoupling pyrolysis and gasification into two distinct stages. In current work, the pyrolysis and gasification characteristics of microalgae Nannochloropsis sp. with calcium ferrite as oxygen carrier were performed by TG-FTIR analysis and e ...
DOI:
10.1016/j.jaap.2021.105129

81. Combination of Synergic Enzymes and Ultrasounds as an Effective Pretreatment Process to Break Microalgal Cell Wall and Enhance Algal Oil Extraction

Author:
Cristina Blanco-Llamero; Paz García-García; Francisco Javier Señoráns
Source:
Foods 2021 v.10 no.8 pp. -
ISSN:
2304-8158
Subject:
Nannochloropsis; algal oils; biomass; cell walls; dietary supplements; glycolipids; microalgae; pH; sonication; subtilisin; synergism; ultrasonic treatment; ultrasonics
Abstract:
... Microalgal biomass is a sustainable source of bioactive lipids with omega-3 fatty acids. The efficient extraction of neutral and polar lipids from microalgae requires alternative extraction methods, frequently combined with biomass pretreatment. In this work, a combined ultrasound and enzymatic process using commercial enzymes Viscozyme, Celluclast, and Alcalase was optimized as a pretreatment met ...
DOI:
10.3390/foods10081928

82. Comparative indexes, fuel characterization and thermogravimetric- Fourier transform infrared spectrometer-mass spectrogram (TG-FTIR-MS) analysis of microalga Nannochloropsis Oceanica under oxidative and inert torrefaction

Author:
Congyu Zhang; Shih-Hsin Ho; Wei-Hsin Chen; Rupeng Wang
Source:
Energy 2021 v.230 pp. 120824
ISSN:
0360-5442
Subject:
Fourier transform infrared spectroscopy; Nannochloropsis; biofuels; elemental composition; energy efficiency; lignite; microalgae; palmitoleic acid; peat; pyrolysis; torrefaction
Abstract:
... The torrefaction performances of microalga Nannochloropsis Oceanica under oxidative and inert atmospheres are characterized and compared with each other based on several operating parameters. By conducting several comparative indexes, the results suggest that oxidative torrefaction is more capable of upgrading microalgae due to its relatively lower solid yield and energy input, as well as relative ...
DOI:
10.1016/j.energy.2021.120824

83. Comparative study of different algae pyrolysis using photoionization mass spectrometry and gas chromatography/mass spectrometry

Author:
Qi Niu; Jinglan Wang; Congcong Cao; Zhanjun Cheng; Yanan Zhu; Wu Wen; Jian Wang; Yang Pan; Beibei Yan; Guanyi Chen; Frederik Ronsse
Source:
Journal of analytical and applied pyrolysis 2021 v.155 pp. 105068
ISSN:
0165-2370
Subject:
Nannochloropsis; Sargassum; Spirulina; alkenes; cellulose; comparative study; cycloaddition reactions; dehydrogenation; hemicellulose; heterocyclic nitrogen compounds; pyrolysis
Abstract:
... Pyrolysis of three algae, i.e. Nannochloropsis, Spirulina, and Sargassum was investigated by the combination of fixed bed pyrolysis with gas chromatography/mass spectrometry and pyrolysis photoionization mass spectrometry (py-PIMS) methods. Lipid, protein, and carbohydrate are the dominant components of the three algae, respectively. Mass spectrum profiles at different temperatures (400 °C, 500 °C ...
DOI:
10.1016/j.jaap.2021.105068

84. Data on Growth, survivability, water quality and hemato-biochemical indices of Nile Tilapia (Oreochromis niloticus) fry fed with selected marine microalgae

Author:
Kafia Islam Amira; Mohammad Redwanur Rahman; Suchandan Sikder; Helena Khatoon; Jinat Afruj; Mohammad Ekramul Haque; Tashrif Mahmud Minhaz
Source:
Data in Brief 2021 v.38 pp. 107422
ISSN:
2352-3409
Subject:
Nannochloropsis; Oreochromis niloticus; Tetraselmis; body weight; environmental factors; fish meal; glass; hematology; microalgae; photometry; survival rate; water quality
Abstract:
... Data of this article describes growth, survival rate, water quality and hemato-biochemical indices of Nile Tilapia (Oreochromis niloticus) fry. To collect the data, the Nile Tilapia fry was reared in 30 L glass aquarium (18 fish/ tank) for 56-days under controlled environmental condition. Feed was prepared with 25 and 50% replacement of commercial fish meal with Nannochloropsis sp. and Tetraselmis ...
DOI:
10.1016/j.dib.2021.107422

85. Data on growth, productivity, pigments and proximate composition of indigenous marine microalgae isolated from Cox's Bazar Coast

Author:
Zahidul Islam; Helena Khatoon; Tashrif Mahmud Minhaz; Mohammad Redwanur Rahman; Shanur Hasan; Yahia Mahmud; Md Shahadat Hossain; Joyshri Sarker
Source:
Data in Brief 2021 v.35 pp. 106860
ISSN:
2352-3409
Subject:
Chaetoceros; Chlorella; Nannochloropsis; Tetraselmis; absorbance; carbohydrates; carotenoids; chlorophyll; coasts; culture media; growth curves; growth performance; lipids; microalgae; nutrient content; phycobiliprotein; proximate composition
Abstract:
... Data on growth, productivity, pigments and proximate composition of the four different indigenous marine microalgae (isolated from Cox's Bazar Coast) were collected to compare the growth performance, pigments and nutritional composition. Chlorella sp., Nannochloropsis sp., Tetraselmis sp. and Chaetoceros sp. are the four different marine microalgae. Growth curve was determined as the prerequisite ...
DOI:
10.1016/j.dib.2021.106860

86. Determination of transgene stability in Nannochloropsis sp. transformed with immunogenic peptide for oral vaccination against vibriosis

Author:
Aisamuddin Ardi Zainal Abidin; Nur Adila Othman; Fatimah Md. Yusoff; Zetty Norhana Balia Yusof
Source:
Aquaculture international 2021 v.29 no.2 pp. 477-486
ISSN:
0967-6120
Subject:
DNA; Nannochloropsis; RNA; Vibrio; aquaculture; fish feeds; genetically modified organisms; microalgae; oral vaccination; outer membrane proteins; peptides; protein kinases; risk; transgenes; vaccines; vibriosis
Abstract:
... Vibriosis is one of the common diseases caused by gram-negative bacteria from the genus Vibrio. To treat vibriosis, vaccination has been proven to be the most effective treatment as it can avoid the risk of drugs or antibiotics resistance. Microalgae are commonly used as feed for aquatic organisms and Nannochloropsis sp. is one of the highly utilized species for fish feed. This study focused on th ...
DOI:
10.1007/s10499-020-00634-w

87. Development of High-Protein Vegetable Creams by Using Single-Cell Ingredients from Some Microalgae Species

Author:
Fatma Boukid; Josep Comaposada; Albert Ribas-Agustí; Massimo Castellari
Source:
Foods 2021 v.10 no.11 pp. -
ISSN:
2304-8158
Subject:
Chlorella vulgaris; Nannochloropsis; Tetraselmis chuii; color; cream; microalgae; pH; principal component analysis; protein content; vegetables; water activity; water content
Abstract:
... The aim of this paper was to develop high-protein vegetable creams through the incorporation of microalgae. Single-cell ingredients from Arthrospiraplatensis (spirulina), Chlorella vulgaris, Tetraselmis chui, and Nannochloropsis oceanica were incorporated at two levels of addition (1.5% and 3.0%) to a standard vegetable cream (STD). Effects of incorporation were assessed in terms of physicochemica ...
DOI:
10.3390/foods10112550

88. Development of a Phaeodactylum tricornutum biorefinery to sustainably produce omega-3 fatty acids and protein

Author:
Yi Cui; Skye R. Thomas-Hall; Elvis T. Chua; Peer M. Schenk
Source:
Journal of cleaner production 2021 v.300 pp. 126839
ISSN:
0959-6526
Subject:
Nannochloropsis; Phaeodactylum tricornutum; biomass; biorefining; cost effectiveness; eicosapentaenoic acid; ethanol; humans; magnesium; microalgae; oils; omega-3 fatty acids; photobioreactors; protein content
Abstract:
... Phaeodactylum tricornutum is a genetically well-studied microalga but commercial production of omega-3 fatty acids and protein had not been established. Here, a biorefinery platform to cost-effectively culture, harvest and process this diatom has been developed. Using a sea salt substitute supplemented with magnesium enabled low-cost land-based cultivation, reaching biomass yields of 0.17 g L⁻¹day ...
DOI:
10.1016/j.jclepro.2021.126839

89. Development of the “Applied Proteomics” Concept for Biotechnology Applications in Microalgae: Example of the Proteome Data in Nannochloropsis gaditana

Author:
Rafael Carrasco-Reinado; María Bermudez-Sauco; Almudena Escobar-Niño; Jesús M. Cantoral; Francisco Javier Fernández-Acero
Source:
Marine drugs 2021 v.20 no.1 pp. -
ISSN:
1660-3397
Subject:
Nannochloropsis; antineoplastic activity; biotechnology; microalgae; proteome; proteomics
Abstract:
... Most of the marine ecosystems on our planet are still unknown. Among these ecosystems, microalgae act as a baseline due to their role as primary producers. The estimated millions of species of these microorganisms represent an almost infinite source of potentially active biocomponents offering unlimited biotechnology applications. This review considers current research in microalgae using the “omi ...
DOI:
10.3390/md20010038

90. Different Dietary N-3 Polyunsaturated Fatty Acid Formulations Distinctively Modify Tissue Fatty Acid and N-Acylethanolamine Profiles

Author:
Elisabetta Murru; Paula A. Lopes; Gianfranca Carta; Claudia Manca; Armita Abolghasemi; José L. Guil-Guerrero; José A. M. Prates; Sebastiano Banni
Source:
Nutrients 2021 v.13 no.2 pp. -
ISSN:
2072-6643
Subject:
Buglossoides arvensis; Nannochloropsis; Schizochytrium; adipose tissue; arachidonic acid; brain; correlation; docosahexaenoic acid; eicosapentaenoic acid; fish oils; linseed oil; liver; metabolites; microalgae; milk; omega-3 fatty acids; palmitic acid; peas; rats; soybean oil; stearic acid
Abstract:
... We investigated the influence of different dietary formulation of n-3 polyunsaturated fatty acids (PUFA) on rat tissue fatty acid (FA) incorporation and consequent modulation of their bioactive metabolite N-acylethanolamines (NAE). For 10 weeks, rats were fed diets with 12% of fat from milk + 4% soybean oil and 4% of oils with different n-3 PUFA species: soybean oil as control, linseed oil rich in ...
DOI:
10.3390/nu13020625

91. Direct Air Capture of CO₂ by Microalgae with Buoyant Beads Encapsulating Carbonic Anhydrase

Author:
Xiaoyin Xu; Sandra E. Kentish; Gregory J. O. Martin
Source:
ACS sustainable chemistry & engineering 2021 v.9 no.29 pp. 9698-9706
ISSN:
2168-0485
Subject:
Nannochloropsis salina; air; biomass production; calcium alginate; carbon; carbon dioxide; carbonate dehydratase; crosslinking; dyeing; encapsulation; feedstocks; glutaraldehyde; green chemistry; greenhouse gases; microalgae; photosynthesis
Abstract:
... Microalgae cultures have promise as a CO₂ sink for atmospheric carbon and as a sustainable source of food and chemical feedstocks. However, large-scale microalgae cultivation is currently limited by the need to provide carbon dioxide from point sources, as the diffusion of atmospheric CO₂ is too slow. Carbonic anhydrase (CA) is an effective enzyme to facilitate the dissolution of atmospheric CO₂ t ...
DOI:
10.1021/acssuschemeng.1c01618

92. Droplet-based microfluidic screening and sorting of microalgal populations for strain engineering applications

Author:
Ziyi Yu; Katrin Geisler; Tina Leontidou; Rosanna E.B. Young; Sofie E. Vonlanthen; Saul Purton; Chris Abell; Alison G. Smith
Source:
Algal research 2021 v.56 pp. 102293
ISSN:
2211-9264
Subject:
Nannochloropsis; Phaeodactylum tricornutum; chlorophyll; encapsulation; fluorescence; genetic transformation; green fluorescent protein; microalgae; phenotype; research
Abstract:
... The application of microfluidic technologies to microalgal research is particularly appealing since these approaches allow the precise control of the extracellular environment and offer a high-throughput approach to studying dynamic cellular processes. To expand the portfolio of applications, here we present a droplet-based microfluidic method for analysis and screening of Phaeodactylum tricornutu ...
DOI:
10.1016/j.algal.2021.102293

93. Edible Microalgae and Their Bioactive Compounds in the Prevention and Treatment of Metabolic Alterations

Author:
Sara Ramos-Romero; Joan Ramon Torrella; Teresa Pagès; Ginés Viscor; Josep Lluís Torres
Source:
Nutrients 2021 v.13 no.2 pp. -
ISSN:
2072-6643
Subject:
Arthrospira; Chlorella; Isochrysis; Nannochloropsis; Spirulina; Tetraselmis; agricultural land; antioxidants; biomass; carbon dioxide fixation; carotenoids; diabetes; docosahexaenoic acid; eicosapentaenoic acid; folic acid; freshwater; human nutrition; humans; insulin resistance; knowledge; land use; metabolic syndrome; microalgae; obesity; paper; peptides; phenolic compounds; phycocyanin; polysaccharides; prebiotics
Abstract:
... Marine and freshwater algae and their products are in growing demand worldwide because of their nutritional and functional properties. Microalgae (unicellular algae) will constitute one of the major foods of the future for nutritional and environmental reasons. They are sources of high-quality protein and bioactive molecules with potential application in the modern epidemics of obesity and diabete ...
DOI:
10.3390/nu13020563

94. Effect of Hg2+ on the microphysical and chemical properties of oil-producing Nannochloropsis sp.

Author:
Jing Sun; Jun Cheng; Zongbo Yang; Junhu Zhou
Source:
Algal research 2021 v.60 pp. 102525
ISSN:
2211-9264
Subject:
Nannochloropsis; adsorption; bioenergy; carboxylic acids; cell walls; heavy metals; lipid content; microalgae; oils; porosity; research; sewage treatment; surface area
Abstract:
... The influence of the adsorption of heavy metal Hg²⁺ on the microphysical and chemical properties of microalgal cells and the content and composition of oil were analysed in this paper. Experimental studies showed that the microalgal cells that adsorbed heavy metals showed grooves and folds on the surface of the cell wall and had 5.8 times increase in specific surface area, 59.09% decrease in pore ...
DOI:
10.1016/j.algal.2021.102525

95. Effect of ammonium formate washing on the elemental composition determination in Nannochloropsis oceanica

Author:
Bárbara O. Guimarães; Pieter Gremmen; René H. Wijffels; Maria J. Barbosa; Sarah D'Adamo
Source:
Aquaculture 2021 v.538 pp. 736526
ISSN:
0044-8486
Subject:
Nannochloropsis; ammonium; aquaculture; biomass; cell culture; centrifugation; culture media; elemental composition; equipment; formates; microalgae; spectroscopy
Abstract:
... This study investigated the effect of salt presence and removal on the integrity and elemental determination of the marine microalgae Nannochloropsis oceanica. Cells were harvested by centrifugation and washed with ammonium formate five times and subjected to mineral analysis after each washing step. Supernatants from each washing step were also analysed. Ca, Cu, Fe, Mg, Mn, Na, P, K, S, Zn were d ...
DOI:
10.1016/j.aquaculture.2021.736526

96. Effect of density, temperature and diet on the growth, survival and development of larvae and juveniles of Isostichopus sp.

Author:
Ernesto J. Acosta; Adriana Rodríguez‐Forero; Bernd Werding; Andreas Kunzmann
Source:
Aquaculture research 2021 v.52 no.2 pp. 611-624
ISSN:
1355-557X
Subject:
Chaetoceros calcitrans; Isochrysis galbana; Isostichopus; Nannochloropsis; Pavlova; Tetraselmis chuii; aquaculture; diet; larvae; metamorphosis; microalgae; mortality; new species; research; temperature; Caribbean; Colombia
Abstract:
... Isostichopus sp. is a variety of Isostichopus badionotus, proposed as a new species, which has been intensely fished in the Colombian Caribbean, arousing interest for its aquaculture. This study evaluated the effect of two culture densities (1–3 larvae ml⁻¹), two temperatures (23°C and 26°C) and two microalgae diets (Isochrysis galbana, Chaetoceros calcitrans, Nannochloropsis oculata 1:1:1 and I. ...
DOI:
10.1111/are.14918

97. Effect of high pressure homogenization on microstructural and rheological properties of A. platensis, Isochrysis, Nannochloropsis and Tetraselmis species

Author:
Johannes Magpusao; Stephen Giteru; Indrawati Oey; Biniam Kebede
Source:
Algal research 2021 v.56 pp. 102327
ISSN:
2211-9264
Subject:
Isochrysis; Nannochloropsis; Tetraselmis; biomass; food composition; homogenization; hydrophilicity; hydrophobicity; microalgae; nutrient content; particle size; research; viscosity
Abstract:
... The rich nutritional profile of microalgae biomass has made it a promising clean label food ingredient that has the potential to fortify incorporated food with simultaneous modification of the structural properties. This work investigated the effect of high pressure homogenization (HPH) at 300, 600, and 900 bar on the microstructural and rheological properties of four microalgal suspensions, namel ...
DOI:
10.1016/j.algal.2021.102327

98. Effect of the inclusion of microalgae on the physical properties of extruded feed for gilthead seabream (Sparus aurata L.)

Author:
R. Alcaraz; A. Hernández-Contreras; P. Iglesias; M.D. Hernández
Source:
Algal research 2021 v.53 pp. 102167
ISSN:
2211-9264
Subject:
Chlorella vulgaris; Nannochloropsis; Sparus aurata; Tetraselmis chuii; density; diet; durability; fish feeds; gelatinization; hardness; ingredients; microalgae; mixtures; pellets; research; starch; temperature profiles; velocity; water solubility; water uptake
Abstract:
... The inclusion of new ingredients in fish feed can affect the physical quality of the pellets. In order to study the effect of the addition of microalgae on the physical properties of the pellets, 15 extruded diets were studied that included three different species of microalgae (Nannochloropsis gaditana, Tetraselmis chuii and Chlorella vulgaris) at five different inclusion levels (0, 2.5, 5, 10 an ...
DOI:
10.1016/j.algal.2020.102167

99. Effective Cu/Re promoted Ni-supported γ-Al2O3 catalyst for upgrading algae bio-crude oil produced by hydrothermal liquefaction

Author:
Kanokthip Pongsiriyakul; Worapon Kiatkittipong; Sushil Adhikari; Jun Wei Lim; Su Shiung Lam; Kunlanan Kiatkittipong; Apiwat Dankeaw; Prasert Reubroycharoen; Navadol Laosiripojana; Kajornsak Faungnawakij; Suttichai Assabumrungrat
Source:
Fuel processing technology 2021 v.216 pp. 106670
ISSN:
0378-3820
Subject:
Nannochloropsis; alcohols; alloys; amination; biofuels; biomass; carbon; catalysts; energy recovery; feedstocks; hydrogenation; hydrothermal liquefaction
Abstract:
... Catalytic hydrotreating of algae bio-crude oil from hydrothermal liquefaction (HTL) of Nannochloropsis sp. was performed. Different Ni-based catalysts, including Ni/γ-Al₂O₃, Ni-Cu/γ-Al₂O₃, Ni-Re/γ-Al₂O₃, and Ni-Cu-Re/γ-Al₂O₃, (10%Ni, 5%Cu, 2.5%Re) were used in upgrading of the bio-crude oil. Most catalytic systems could effectively eliminate S and decrease the N and O contents, and enhance more th ...
DOI:
10.1016/j.fuproc.2020.106670

100. Effects of Chlorella vulgaris, Nannochloropsis oceanica and Tetraselmis sp. supplementation levels on in vitro rumen fermentation

Author:
Denis J. Meehan; Ana R.J. Cabrita; Joana L. Silva; António J.M. Fonseca; Margarida R.G. Maia
Source:
Algal research 2021 v.56 pp. 102284
ISSN:
2211-9264
Subject:
Chlorella vulgaris; Nannochloropsis; Tetraselmis; cell walls; corn silage; diet; digestive tract; energy; fermentation; haylage; inoculum; methane; methane production; microalgae; nitrogen; nonprotein nitrogen; polysaccharides; research; ruminant nutrition
Abstract:
... Microalgae constitute an underexploited feed resource for ruminant nutrition. The unique digestive tract of ruminants makes them best suited to utilise complex polysaccharides and non-protein nitrogen of eukaryotic microalgae, though contrasting effects on rumen fermentation have been reported. The current study aimed to assess the effects of microalgae species, inclusion level and basal substrate ...
DOI:
10.1016/j.algal.2021.102284