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... The impact of insect artificial diet on the egg production of females was examined for L29 consequently generations of laboratory populations Perillus bioculatus (F.) (Hemiptera: Pentatomidae, Asopinae). Particular attention is paid to the overwintered generation, which plays a key role in the rehabilitation of the predator populations after hibernation. It was shown that with an increase in the n ...
... Stage specific mortality and sex ratio adjustment in Zygogramma bicolorata Pallister (Coleoptera: chrysomelidae), were evaluated monthly for two consecutive years, 2010 and 2011, in field conditions on Parthenium hysterophorus L. Development of immature stages was considerably affected during the cold seasons of 2010 and 2011, and it was completely arrested in January. Similarly, the mortality sur ...
Coccinella septempunctata; Hippodamia convergens; Leptinotarsa decemlineata; Perillus bioculatus; eggs; larvae; life tables; methyl salicylate; mortality; natural enemies; phytophagous insects; population dynamics; potatoes; predatory arthropods; Virginia
Abstract:
... Methyl salicylate is an organic compound produced by potato and other plants in response to insect herbivory. In other studies the compound has been shown to be attractive to numerous predatory arthropods. Experiments were conducted in Virginia to evaluate methyl salicylate lures for attracting natural enemies in potato plots to manage populations of Leptinotarsa decemlineata (Say). Abundance of p ...
... The paper reviews the diversity of seasonal cycles known in stink bugs (Heteroptera, Pentatomidae) from the temperate zone and is based on the data of 43 pentatomid species studied in detail up to date (Saulich and Musolin, 2011). All the seasonal cycles realized by pentatomids in the temperate zone can be divided into two large groups: univoltine and multivoltine cycles. In univoltine cycles, onl ...
... Marking biological control agents facilitates studies of dispersal and predation. This study examines the effect of a biological solvent, dimethyl sulfoxide (DMSO), on retention of immunoglobulin G (IgG) protein solutions applied to Diorhabda carinulata (Desbrochers) (Coleoptera: Chrysomelidae), an important biological control agent of saltcedar, either internally by feeding them protein‐labeled f ...
Ambrosia artemisiifolia; Leptinotarsa decemlineata; Perillus bioculatus; acclimation; biological control; biological control agents; habitats; periodicity; phytophagous insects; weeds; Russia
Abstract:
... The explosive spread of the common ragweed induced by disturbance of the natural habitats in the postwar years created an ideal vacant niche for acclimation of North American phytophagous insects introduced to the South of Russia in the 1960–70s. The propagation of these species resulted in restoration of natural succession periodicity and promoted the subsequent acclimation of the predatory stink ...
DNA; Leptinotarsa decemlineata; Perillus bioculatus; biological control; phylogeny; random amplified polymorphic DNA technique; reproduction
Abstract:
... The biology and bioregulatory activity of a population of the North American predatory two-spotted stink bug Perillus bioculatus F. are studied and the genetic similarity between this species of predatory bugs and other representatives of the family Pentatomidae is analyzed. It is established that P. bioculatus is of great importance for biological control of the Colorado potato beetle by the natu ...
... Froeschner (1988) records 23 species of stink bugs (Hemiptera: Heteroptera: Pentatomidae) as occurring in Washington state. Based onmaterial primarily housed in the M.T. James Entomological Collection at Washington State University, the number of species is increased to 47. Species recorded by Froeschner (1988) but not found in our collections are: Apateticus crocatus (Uhler), Chlorochroa rossiana ...
... Determining relative strengths of trophic links is critical for ranking predators for conservation biological control. Molecular gut-content analysis enables ranking by incidence of prey remains in the gut, but differential digestive rates bias such rankings toward predators with slower rates. This bias can be reduced by indexing each predator's half-life to that of the middle-most half-life in a ...
... Habitat manipulation in agroecosystems can influence predator–prey interactions. In this study, we collected foliar predators from field potato plots with different mulch treatments and assayed them for DNA of the target prey, Leptinotarsa decemlineata (Say), using species-specific primers. Concurrently, L. decemlineata larval abundance and plant damage were recorded from the same plots. Predator ...
... Cold storage of insects is a common practice used when transporting insects or to assist with efforts to increase the size of a colony. However, cold storage affects egg, nymphal and adult survival, fecundity, and egg viability. We evaluated the influence of nutrient source on the response of eggs, nymphs and adults of Perillus bioculatus when exposed to two temperatures most likely to be used for ...
... Plant cystatins, similar to other defense proteins, include hypervariable, positively selected amino acid sites presumably impacting their biological activity. Using 29 single mutants of the eighth domain of tomato (Solanum lycopersicum) multicystatin, SlCYS8, we assessed here the potential of site-directed mutagenesis at positively selected amino acid sites to generate cystatin variants with impr ...
... Fragments of two artificial diet up-regulated and two prey up-regulated transcripts were isolated from the predatory Pentatomid Perillus bioculatus using suppression subtractive hybridization. A BlastX search found similarities for two diet-upregulated clones, i.e., the tyrosine-3-monooxygenase gene and the gene for the chitin binding protein, Gasp. The probe generated from the tyrosine-3-monooxyg ...
... Recent developments in the area of nutrigenomics hold the promise of providing valuable information about the impact of nutrition on a wide range of biochemical parameters by investigating how nutrition alters global gene expression patterns. Our goal is to use a nutrigenomics approach to identify insect molecular markers that could be used as early indicators of insect responses to different nutr ...
predatory insects; biological control agents; developmental stages; Solanum tuberosum; group size; body size; Leptinotarsa decemlineata; predation; Perillus bioculatus; insect development; mortality; predator-prey relationships
Abstract:
... Nymphs of Perillus bioculatus F., a specialist pentatomid predator of the Colorado potato beetle, Leptinotarsa decemlineata (Say), are smaller than comparable instars of their prey. In laboratory experiments, P. bioculatus fed small prey exhibited greater capture success, increased survivorship, and faster development to adulthood than individuals fed large prey. An increase of potato beetle prey ...
... Selected morphological and physiological properties of the corpus allatum (CA)––corpus cardiacum (CC) complex from the two-spotted stinkbug, Perillus bioculatus (Hemiptera: Pentatomidae), were studied. The CAs play an important role in insect physiology because of their production of the juvenile hormones (JHs), i.e., key hormones involved in development and reproduction. We found that the P. bioc ...
... The effect of juvenile hormone (JH) III on the hemolymph composition of vitellogenin was examined in Perillus bioculatus. Adult females were treated topically with JH III, and the premature presence of vitellogenin in the hemolymph was then detected using electrophoresis and Western blot analyses. JH III treatment resulted in a dose-dependent early production of vitellogenin that was detectable 48 ...
... The impact of a zoophytogenous, insect-free artificial diet on the developmental rate, life history parameters, and fertility was examined over 11 consecutive generations for domesticated Perillus bioculatus (F.) (Heteroptera: Pentatomidae). This study showed that when fed an insect-free artificial diet during both the nymphal and adult stages, developmental times were prolonged, and the net repro ...
... Perillus bioculatus (F.) can be maintained in the laboratory on a diet of Heliothis virescens F. larvae if supplemented with Colorado potato beetle (CPB) eggs. Here we demonstrate that an artificial diet can replace the CPB eggs and maintain the colony for at least three generations. This enables us to maintain our Perillus colonies at high numbers independent of the normal fluctuations in our CPB ...
... Control females of the twospotted stink bug, Perillus bioculatus (F.), and those given an artificial diet mated repeatedly over their lifetimes when held with males. The average number of observed matings was 4 and 8.4 in the controls and those given the artificial diet, respectively. Mating and an adequate diet are prerequisites for laying a full compliment of eggs in this insect. Virgins laid an ...
ovarian follicles; artificial diets; biological control agents; age; predators; ovarioles; sexual reproduction; Perillus bioculatus; mating behavior; vitellogenesis
Abstract:
... A method is presented to quantitatively score the degree of ovarian maturation in a predacious pentatomid with asynchronous follicle development. The effects of artificial diet and mating status on ovarian maturation rates were examined. Ovarian scores were not influenced by mating status but were significantly lower in females fed the artificial diet. Ovarian follicles start forming when females ...
... Plant resistance to insect pests based on recombinant proteinase inhibitors (PIs) could interfere with natural enemies of target pests, as their own proteolytic systems may also be sensitive to large spectrum PIs. Oryzacystatin I (OCI) is potential insect pest resistance factor currently engineered into a variety of crop plants, including potato Solanum tuberosum. Potential for OCI interfering wit ...
... Seven agricultural gels were tested as potential carriers for the eggs of 2 predators, Perillus bioculatus (F.) and Podisus maculiventris (Say) (Heteroptera: Pentatomidae). Gels were tested for their effects on egg hatch in petri dishes, covered and uncovered, using 3 viscosities. Egg hatch in petri dishes was generally higher in polyacrylamide gels (Aquastore F and Terra-sorb GB) and starch-based ...
... Sublethal effects of Bacillus thuringiensis on pests could synergize with natural enemies through extended larval development and reduced defense against predators. Mortality ranged from 18 to 76% for 4th instar Colorado potato beetles feeding on potato foliage dipped in M-Trak at 0.2-3.2 ml/liter H2O. Survivors resumed feeding in 48 h, but feeding was prolonged and time to pupation was extended 3 ...
biological control agents; developmental stages; ambient temperature; predators; Leptinotarsa decemlineata; Perillus bioculatus; group effect
Abstract:
... The dispersal potential of natural enemies is critical to the success of biological control by mass release. Movement of Perillus bioculatus (F), a predator of the Colorado potato beetle, Leptinotarsa decemlineata (Say), was studied under both laboratory and field conditions. Factors studied were temperature, number of P. bioculatus nymphs released, their nymphal stage and physiological age affect ...
... In laboratory and microplot consumption tests, spined soldier bug, Podisus maculiventris (Say), ate at least as many Colorado potato beetle, Leptinotarsa decemlineata (Say), eggs and larvae as did the twospotted stink bug, Perillus bioculatus (F.). P. maculiventris also remained in field microplots infested with Colorado potato beetle egg masses for slightly longer than did P. bioculatus. The 2 pr ...
... Field studies during 1986-1988 in southwestern Michigan showed that horsenettle, Solanum carolinense L., could be an important reservoir of Colorado potato beetle, Leptinotarsa decemlineata (Say), populations when potatoes, Solanum tuberosum L., are not planted. Early in the season, Colorado potato beetle laid most of its eggs on nonhost plants. Accumulated total larval mortality ranged between 82 ...
... Fifth instars of the hemipteran predator Perillus bioculatus (F.) were observed as they attacked neonate Leptinotarsa decemlineata (Say) larvae on potato plants. Observations were conducted at prey densities ranging from one to 100 prey per plant, and standard functional response models were fit to the predation rates obtained. Neither the type II nor the type III functional-response curves provid ...
Perillus bioculatus; Solanum tuberosum; Podisus maculiventris; predators; Leptinotarsa decemlineata; population density; larvae; insect control; mortality; biological control
Abstract:
... Field cage tests in 1987 established that releases of 5-10 Perillus bioculatus (F.) and Podisus macutiventris (Say) per plant were able to reduce high density populations (approximately 450 per plant) of Colorado potato beetle by approximatey 50%. P. bioculatus greater foliage protection than P. maculiventris. In 1988, in similar cage tests, P. bioculatus released at rates of 2-8 per plant reduced ...
... Endosulfan, oxamyl, and esfenvalarate all were highly toxic to Perillus bioculatus (F.), a predator of the Colorado potato beetle, Leptinotarsa decemlineata (Say). Toxicity assays consisted of topical application to eggs, nymphs, and adults; exposure of nymphs to treated foliage; and ingestion by P. bioculatus nymphs of Colorado potato beetle larvae previously exposed to treated foliage. P. biocul ...
sex pheromones; Oplomus dichrous; bioassays; spectral analysis; Perillus bioculatus; synthesis
Abstract:
... Racemic 6,10,13-trimethyl-1-tetradecanol has been synthesized. Successive elaboration of the molecule using 5-methyl-2-hexanone as the carbon-terminal fragment gave the desired alcohol in a 4% overall yield over 13 steps. Neglecting stereochemistry, the product was shown to be identical to the alcohol previously identified from the sternal glands of maleStiretrus anchorago. It attracts both adult ...