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Haematococcus pluvialis; Nannochloropsis; Porphyridium cruentum; antioxidants; bioactive compounds; carotenoids; cholinesterase; cytokines; cytotoxicity; health promotion; microalgae; research
Abstract:
... Microalgae represent one of the most promising sources of bioactive compounds for the pharmaceutical and food industries. Among these bioactives, carotenoids could be highlighted due to their health-promoting effects, including anti-inflammatory, neuroprotective or antioxidant properties. In this study, diverse microalgae extracts enriched in carotenoids, including extracts from Haematococcus pluv ...
Rocio Janeth Aguilar‐Ruiz; Dalia Isabel Sánchez‐Machado; María del Rosario Martinez‐Macias; Jaime López‐Cervantes; Omar Nateras‐Ramírez; Olga Nydia Campas‐Baypoli; Yedidia Villegas‐Peralta
Nannochloropsis; Porphyridium cruentum; alpha-tocopherol; amino acids; aquaculture; carbohydrates; culture media; light intensity; microalgae; nutritive value; protein content; research
Abstract:
... Microalgae are considered sources of compounds of high nutritional value. This study determined whether 400 μE/m²/s (HL) and 80 μE/m²/s (LL) light intensities and algal medium (AM) and f/2 Guillard medium (f/2GM) influence kinetic parameters, moisture, ash, lipids, carbohydrate, protein, amino acid, vitamins contents of the microalgae Nannochloropsis oculata and Porphyridium cruentum. N. oculata r ...
... Changes in light wavelength can alter the phycobiliprotein content in photosynthetic organisms. These molecules are involved in harvesting light energy for photosynthesis and are interesting microalgal products. Furthermore, increasing or modifying phycobiliprotein content in eukaryotic microalgae is possible. Specifically, exposure to different wavelengths triggers their ability to switch their m ...
... A novel and green one-step simultaneous extraction process of phycobiliproteins and polyunsaturated fatty acids (PUFAs) from wet Porphyridium biomass has been done and optimized by using three phase partitioning (TPP) process. Results showed that the coupling of ammonium sulfate and protein buoyancy-promoting t-butanol afforded the best TPP to extract phycobiliproteins and PUFAs in term of the ext ...
Miroslava Anna Šefcová; Francisco Santacruz; César Marcelo Larrea-Álvarez; Christian Vinueza-Burgos; David Ortega-Paredes; Gabriel Molina-Cuasapaz; Jessica Rodríguez; William Calero-Cáceres; Viera Revajová; Esteban Fernández-Moreira; Marco Larrea-Álvarez
... This pilot investigation aimed at studying the feasibility of using a low dose (0.2%) of dietary microalgae as a means of improving intestinal morphometry, body weight, and selected meat quality parameters in broilers. A total of 72 one-day-old ROSS 308 male chicks were randomly separated into four groups; three experimental pens in which the birds were fed with biomass from Tysochrysis lutea, Tet ...
... The exopolysaccharides (EPS) excreted by the unicellular red alga, Porphyridium, have potential applications in medicine, cosmetics, and health care products. In the present study, two different Porphyridium stains, i.e., Porphyridium cruentum CCALA 415 and Porphyridium purpureum FACHB 806, were selected and their EPS productivity, chemical characteristics, and antioxidant activities were compared ...
... This study aimed to investigate the harvesting of microalgae by microfiltration (MF) on a ceramic membrane at relatively high cross-flow velocity (CFV) of interest for commercial processes. Pilot-scale harvesting was conducted with algal suspensions (Chlorella vulgaris and Tisochrysis lutea (T-Iso)) and algal supernatants (Porphyridium cruentum) to assess the effect of feedstock characteristics an ...
... Phycobiliproteins are colored, active molecules with potential use in different industries. They are the union of proteins and bilins (Chromophores). The primary source of phycobiliproteins is algae; however, the traditional algae culture has production restrictions. The production in bacterial models can be a more efficient alternative to produce these molecules. However, the lack of knowledge in ...
... Silver nanoparticles (AgNPs) have gained considerable attention amongst researchers in various applications, owing to their physiochemical and biological properties. Due to disadvantages of previous synthesis methods, various green synthesis methods have been tried recently. In this study, a one-pot method for the synthesis of silver nanoparticles (AgNPs) was investigated through an environmentall ...
... In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production. The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively. Furthermore, simultaneous applicatio ...
DNA; Porphyridium cruentum; antioxidants; cations; cluster analysis; emulsions; exopolysaccharides; free radicals; lipid peroxidation; livestock and meat industry; microorganisms; minced beef; principal component analysis; refrigeration; shelf life; storage time
Abstract:
... Sulfated exopolysaccharides (EPS) from Porphyridium cruentum strain were extracted and their antioxidant and anti-bacterial potentials were evaluated based on DPPH free radical, ABTS•⁺ radical cation and DNA nicking assays, and against four foodborne pathogenic bacteria, respectively. They showed also interesting functional, foaming and emulsion properties. Moreover, microbiological and chemical e ...
Porphyridium cruentum; animal disease models; biomass; blood glucose; body weight; diabetes; food intake; glibenclamide; glycemic effect; health effects assessments; intestines; islets of Langerhans; microalgae; polysaccharides; rats; streptozotocin; villi
Abstract:
... Porphyridium cruentum, known as red microalga, is able to produce extra-cellular polysaccharides (EPs) that have beneficial health effects. In this study, the effect of P. cruentum biomass and EPs with various doses was studied in streptozotocin (STZ)-induced diabetic rats to determine their antihyperglycemic activity and its potential mechanism. The doses of biomass were 600, 1200 and 1800 mg/kg ...
... For the first time, the microalga Porphyridium cruentum was tested for its ability to produce silver nanoparticles. To characterize formed silver nanoparticles UV–vis Spectrometry, Scanning Electron Microscopy, Energy-dispersive analysis of X-rays and X-ray diffraction were used. It was shown that after biomass exposure to silver nitrate solution the extracellular formation of spherical-like nanop ...
... Porphyridium cruentum, a cell-wall-free marine Rhodophyta microalga was cultured under a 5-day cold stress at 0 °C and 15 °C, after reaching the late logarithmic growth phase. Compared with the control at 25 °C, the cold stress treatment significantly (p < 0.05) increased the microalgal biomass (1.21-fold); the amounts of total polyunsaturated fatty acids (1.22-fold); individual fatty acids includ ...
... The effect of co-cultivation of Porphyridium cruentum UTEX 161 with Pseudoalteromonas sp. MEBiC 03485 on P. cruentum growth and its sulfated polysaccharide (EPS) production were examined. The strain MEBiC 03485 had beneficial effects on P. cruentum growth, EPS production, and EPS quality. These effects were due to a compound secreted by the strain MEBiC 03485. Notably, secretory compound treatment ...
... Microalgal biomass is a promising candidate for biogas production, however; cell characteristics may prevent accessibility of anaerobic microorganisms to organic matter and limits methane production from microalgae. Pretreatments provide enhancement in methane yields via cell wall disruption and cell membrane solubilization. In this study, improvement of methane production from Porphyridium cruent ...
... Exopolysaccharides, or extracellular polysaccharides (EPS, sPS), represent a valuable metabolite compound synthesized from red microalgae. It is a non-toxic natural agent and can be applied as an immunostimulant. The toxicity test of exopolysaccharides from Porphyridium has been done in vivo using zebrafish (Danio rerio) embryonic model, or the ZET (zebrafish embryotoxicity test). The administrati ...
... The red microalga Porphyridium cruentum is exploited industrially for its exopolysaccharides (EPS) and pigments production. EPS produced by P. cruentum are partially released and dissolved into the surrounding environment, they can be recovered from the culture medium after removing the cells. This paper presents a parametric study of the ultrafiltration of EPS solutions on organic membrane. The E ...
... Porphyridium cruentum is a versatile cell factory to produce various bioactive and vital substances for food, feed, cosmetics and pharmaceuticals. In this study, a novel two-stage cultivation was the first time to establish for microalgal growth and bioactive compounds production by regulating light-emitting diodes wavelengths using marine microalga Porphyridium cruentum. Firstly, the best light l ...
... Microalgae can potentially be used as a food ingredient due to its biochemical composition that can enrich the bioactive compound content of the food products. In the present study, spray-dried Nannochloropsis oculata, Porphyridium cruentum and Diacronema vlkianum microalgae species were added to ice cream formulation at different concentrations (0.10, 0.20 and 0.30 g 100 g⁻¹). The effect of micro ...
... The carotenoid profiles of biomass from five eukaryotic microalgae, Porphyridium cruentum, Isochrysis galbana, Phaeodactylum tricornutum, Tetraselmis suecica and Nannochloropsis gaditana, produced at an industrial plant in outdoor photobioreactors, were investigated. Pigments were solvent-extracted after an ultrasonic pre-treatment and separated by HPLC-photodiode-array using a reversed-phase C18 ...
... The aim of the study was to extract B-phycoerythrin from Porphyridium cruentum while preserving its structure. The high hydrostatic pressure treatments were chosen as extraction technology. Different methods have been used to observe the effects of the treatment: spectrophotometry and confocal laser scanning microscopy followed by image processing analysis. Image processing led to the generation o ...
Porphyridium cruentum; cosmetics; dyes; electrophoresis; emulsions; fluorescence; high pressure treatment; medicine; microalgae; protein structure; texture; turbidity; yields
Abstract:
... High hydrostatic pressure (HHP) treatments are trending as “green” stabilization and extraction process. The extraction of B-phycoerythrin from microalgae is getting more and more interest due to its numerous potentialities in foods, cosmetics and medicine. Thus, the effects of high pressure on the structural characteristics of B-phycoerythrin extracted from Porphyridium cruentum are explored in t ...
... Microalgae are considered prolific sources of bioactive compounds that can be useful for nutraceuticals. In this study, the potential of ultra-high pressure extraction (UHPE) for the simultaneous cell disruption and extraction of bioactives from two microalgae species, Haematococcus pluvialis and Porphyridium cruentum, was evaluated. The variables studied to extract carotenoids for H. pluvialis we ...
... There is great microalgae biodiversity and their rediscovery as an inexhaustible source of biotechnological resources for all types of applications is leading to the already demonstrated benefits of their bioactive compounds which has boosted their industrial development. For this development, it is essential to have available technologies to easily control large-scale cultures. In this study, a h ...
... In this study, Pavlova lutheri, Chlorella vulgaris, and Porphyridium cruentum were cultured using modified F/2 media in a 1 L flask culture. Various nitrate concentrations were tested to determine an optimal nitrate concentration for algal growth. Subsequently, the effect of light emitted at a specific wavelength on biomass and lipid production by three microalgae was evaluated using various wavel ...
Tom M.M. Bernaerts; Lore Gheysen; Clare Kyomugasho; Zahra Jamsazzadeh Kermani; Stéphanie Vandionant; Imogen Foubert; Marc E. Hendrickx; Ann M. Van Loey
... Microalgae are rich in several nutritional and health-beneficial components, showing great potential as functional food ingredients. To this extent, knowledge of the biomass composition is essential in the selection of suitable microalgae species for specific food applications. Surprisingly, although cell wall polysaccharides are generally reported to play a role in functionality, limited attentio ...
... Exopolysaccharides (EPS) from cell-free Porphyridium cruentum media were concentrated then purified (diafiltration) on a 0.14 μm ceramic membrane. The influence of cross-flow velocities on filtration performances was investigated. Mean permeate fluxes equal to 49.8, 68.9 and 81.9 L h⁻¹. m⁻² were obtained during the concentration at 4 bars for respectively cross-flow velocities inside the membrane ...
... This study systematically examined the effect of nitrogen and phosphorous stress on the formation of linoleic acid (LA), arachidonic acid (ARA), and eicosapentaenoic acid (EPA) in Porphyridium cruentum gy-h56. P. cruentum was cultivated in six different media conferring different conditions of nitrogen (N) sufficiency/deprivation and phosphorous (P) sufficiency/limitation/deprivation. Over a 16-da ...
M. del Pilar Sánchez-Saavedra; Fátima Y. Castro-Ochoa; Viridiana Margarita Nava-Ruiz; Duahmet A. Ruiz-Güereca; Ana Laura Villagómez-Aranda; Fabián Siqueiros-Vargas; Ceres A. Molina-Cárdenas
... We determined the effects of two nitrogen sources (ammonium and nitrate) and two irradiance levels (50 and 200 μmol photons m⁻² s⁻¹) on the growth rate, cell size, proximate composition, pigment content, and photosynthesis of the unicellular red alga, Porphyridium cruentum. Irradiance significantly affects growth rate, as well as carbohydrate, protein, and phycoerythrin content. Nitrogen form sign ...
... BACKGROUND: The selection of hydrodynamic properties of microalgae such as agitation speed is usually done according to empirical values without a technical basis, and the selected agitation speeds are not consistent with each other in many references. In this study, we aimed to provide a possible technical basis for the setup of the agitation speed of microalgae suspensions based on basic rheolog ...
... During the extraction processes of B-phycoerythrin from P. cruentum, the possibility of the occurrence of extraction-inhibiting B-phycoerythrin/exopolysaccharide (EPS) complexes was highlighted. The use of confocal laser scanning microscopy allowed the observation of the cells including their sheath and their chloroplast, the bound EPS matrix, and extracellular vesicles involving phycobiliproteins ...
... Cell wall related polysaccharides of the red microalga Porphyridium sp. were shown to be a promising source of new sustainable thickening agents. Isolated extracellular polysaccharides (EPS) consisted of high molecular weight polymers, showing a higher intrinsic viscosity compared to several commercially used hydrocolloids. Aqueous solutions of EPS (2% w/w) were characterized by substantial viscos ...
Porphyridium cruentum; autolysis; biomass; cell membranes; dissociation; electric field; electroporation; microalgae; permeability; phycoerythrin; pulsed electric fields
Abstract:
... This paper assesses the extraction of β-phycoerythrin (BPE) into aqueous media by the application of pulsed electric field treatments (PEF) to the fresh biomass of Porphyridium cruentum.An increase in electric field strength from 2 to 10 kV/cm, or a prolongation of treatment time increased electroporation and inactivation of P. cruentum in the range investigated (2–10 kV/cm; 30–150 μs). A perfect ...
... Membrane chromatography was exploited to purify allophycocyanin, phycocyanin and B-phycoerythrin from aqueous extracts of fresh or freeze-dried biomass of Arthrospira platensis (Cyanobacteria) and Porphyridium cruentum (Rhodophyta). The method can be tuned to obtain products having various degree of purity and avoids expensive and time consuming column chromatography and ultrafiltration steps. A c ...
... Microalgae biomass are useful resources in biofuel production. The objective of this study was to evaluate bioethanol production in response to Porphyridium cruemtum culture conditions. Enzymatic hydrolysis of seawater P. cruemtum (SPC) and freshwater P. cruemtum (FPC, 1% substrate loading, w/v) resulted in glucose conversion yields of 89.8 and 85.3%, respectively, without any pretreatment. Howeve ...
... Phycoerythrin is the major light‐harvesting pigment protein in red algae and is nowadays widely used as a fluorescent probe in biotechnological applications such as flow cytometry and immunofluorescence microscopy. In addition, it has had substantial economic impact due to its potential as a natural food colorant. However, knowledge on the precise molecular composition of phycoerythrin is limited. ...
... Microalgae are attracting much attention as superior biodiesel producers. In particular, under stressful conditions, they accumulate organic compounds consisting entirely of carbon and hydrogen. The aim of this work was to increase intracellular fatty acid content in Dunaliella tertiolecta (Chlorophyceae), Nannochloropsis oculata (Eustigmatophyceae), and Porphyridium cruentum (Rhodophyceae) using ...
Porphyridium cruentum; aeration; biomass; carbon dioxide; energy use and consumption; exopolysaccharides; light intensity; lighting; methodology; microalgae; mixing; nutrition; photobioreactors; pigments; ponds; viscosity
Abstract:
... Porphyridium cruentum is one of the most valued microalgae species able to produce both pigments and exopolysaccharides. Conventional liquid suspended cultivation in ponds and photobioreactors show its disadvantages in lower cultivation efficiency and higher stirring power consumption due to the high viscosity of the medium by the accumulation of polysaccharides. In this work, a new method of cult ...
... Microalgae are a promising and sustainable source for enhancing the nutritional value of food products. Moreover, incorporation of the total biomass might contribute to the structural properties of the enriched food product. Our previous study demonstrated the potential of Porphyridium cruentum and Chlorella vulgaris as multifunctional food ingredients, as they displayed interesting rheological pr ...
Ângelo Paggi Matos; Rafael Feller; Elisa Helena Siegel Moecke; José Vladimir de Oliveira; Agenor Furigo Junior; Roberto Bianchini Derner; Ernani Sebastião Sant’Anna
... Microalgae contain high levels of proteins, carbohydrates, and lipids, and have found a useful application in enhancing the nutritional value of foods. These organisms can also synthesize long-chain fatty acids in the form of triacylglycerols, such as α-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), linolenic acid (LA), γ-linolenic acid (GLA) and arachidonic acid (A ...
... Microalgae show great potential for use as novel ingredients in food products, as they are rich in several nutritional and health-beneficial components. However, addition of total microalgal biomass might alter the structural properties of the food system. Therefore, information is required about their rheological characteristics towards selection of microalgae species for specific food products. ...
... A method based on normal‐phase high performance liquid chromatography combined with evaporative light‐scattering detector was developed to quantify major neutral lipids from microalgae. A binary gradient enabled a good resolution of hydrocarbons and major neutral lipids by classes in less than 13 min. A good linearity was observed from 0.9 to 30 μg. Limits of detection (LOD) and quantification (LO ...
... The availability of nitrogen is one of the most important determinants that can limit the growth of photosynthetic organisms including plants and algae; however, direct observations on the supramolecular architecture of photosynthetic membranes in response to nitrogen stress are still lacking. Red algae are an important evolutionary group of algae which contain phycobilisomes (PBSs) on their thyla ...
... Marine microorganisms such as phytoplanktons are a rich resource of bioactive components with antioxidant and anti-proliferative activities that can act as novel functional food ingredients. In this study, the pigment profiles, total mycosporine-like amino acids (MAAs) and total phenolic contents (TPCs) in solvent extracts including 90% acetone and methanol from five marine phytoplanktons includin ...
... The large-scale production of microalgae poses a number of challenges, including a costly fertilizer demand. While wastewater provides a concentrated source of nutrients, the presence of biological contaminants and the expense of heat treatment are challenging for large-scale production. The goal of this study was to use ultrafiltration to purify wastewater for use in the cultivation of microalgae ...
... Representatives of phycobiliprotein-containing algae such as Anabaena cylindrica, Anacystis ni- dulans, Gloeotrichia echinulata (Cyanophyceae), Chroomonas spec., Hemiselmis rufescens, Rhodomonas spec. (Cryptophyceae), Porphyridium cruentum, Rhodella violacea (Rhodophyceae) along with the unicellular Cyanidium caldarium (unspecified systematic status) have been investigated for their typical photoa ...
... The presence of thioredoxin was demonstrated in 20 strains of cyanobacteria as well as in one phototrophic bacterium Rhodopseudomonas sulfidophila and in Thiobacillus denitrificans. Thioredoxin activity was not found in Cyanophora paradoxa and in Porphyridium cruentum using the thioredoxin-dependent PAPS-sulfotransferase activity from Synechococcus 6301 as assay system. ...
... The influence of two culture media and three different concentrations of sulphate in the medium on the growth of two strains of Porphyridium cruentum and on the production, composition and viscoelastic characteristics, and antimicrobial properties of the sulphated exopolysaccharide (EPS) were studied.A Bohlin C50 rheometer was used to evaluate the viscosity and elasticity of the EPS solutions. HSV ...
... Variations in the exogenous nitrogen level are known to significantly affect the physiological status and metabolism of microalgae. However, responses of red, green and yellow-green algae to nitrogen (N) availability have not been compared yet. Porphyridium cruentum, Scenedesmus incrassatulus and Trachydiscus minutus were cultured in the absence of N in the medium and subsequent resupply of N to t ...