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1. A Rapid and Sensitive Reverse Transcription–Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Detection of Indian Citrus Ringspot Virus

Author:
Amol D. Kokane; Sunil B. Kokane; Ashish J. Warghane; Mrugendra G. Gubyad; Ashwani Kumar Sharma; M. Krishna Reddy; Dilip Kumar Ghosh
Source:
Plant disease 2021 v.105 no.05 pp. 1346-1355
ISSN:
0191-2917
Subject:
Citrus; Indian citrus ringspot virus; RNA; coat proteins; color; cost effectiveness; cross reaction; genes; pathogens; quarantine; rapid methods; reverse transcriptase polymerase chain reaction; reverse transcription loop-mediated isothermal amplification; India
Abstract:
... Indian citrus ringspot virus (ICRSV) is a devastating pathogen that has a particularly deleterious effect on the ‘Kinnow mandarin’, a commercial citrus crop cultivated in the northwest of India. ICRSV belongs to the Mandarivirus genus within the family of Alphaflexiviridae and has a positive sense single-stranded RNA (ssRNA) genome consisting of six open reading frames (ORFs). Severe cases of ICRS ...
DOI:
10.1094/PDIS-06-20-1349-RE

2. A SYBR green I–based quantitative RT-PCR assay for bovine ephemeral fever virus and its utility for evaluating viral kinetics in cattle

Author:
Shandian Gao; Junzheng Du; Zhancheng Tian; Qingli Niu; Dexuan Huang; Jidong Wang; Jianxun Luo; Guangyuan Liu; Hong Yin
Source:
Journal of veterinary diagnostic investigation 2020 v.32 no.1 pp. 44-50
ISSN:
1943-4936
Subject:
Bovine ephemeral fever virus; RNA; blood sampling; cattle; cross reaction; detection limit; genes; peptidylprolyl isomerase; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; reverse transcription; viruses
Abstract:
... We developed a SYBR green I–based reverse-transcription quantitative PCR (RT-qPCR) assay for bovine ephemeral fever virus (BEFV). Analytical sensitivity of the assay was ~ 100 times higher than conventional RT-PCR. The precision of the RT-qPCR established for RNA standards was high, with intra-assay and inter-assay coefficients of variation of 0.23–0.89% and 0.23–1.02%, respectively. The test was ...
DOI:
10.1177/1040638719895460
PubMed:
31845623
PubMed Central:
PMC7003230

3. A comparative study of marine salmonid alphavirus subtypes 1-6 using an experimental cohabitation challenge model

Author:
Graham, D.A.; Frost, P.; McLaughlin, K.; Rowley, H.M.; Gabestad, I.; Gordon, A.; McLoughlin, M.F.
Source:
Journal of fish diseases 2011 v.34 no.4 pp. 273-286
ISSN:
0140-7775
Subject:
RNA; Salmo salar; Salmon pancreas disease virus; antibodies; cross reaction; disease models; feces; fish; freshwater; heart; histopathology; mucus; neutralization; pancreas; tanks; virus transmission; viruses
Abstract:
... A comparative challenge study of six marine isolates representing subtypes 1-6 of salmonid alphavirus (salmon pancreas disease virus, Genus Alphavirus, Family Togaviridae) was conducted in Atlantic salmon in a fresh water cohabitation trial. Histopathological lesions typical of pancreas disease were observed with all subtypes, and virus was re-isolated from serum of cohabitant fish in each case. U ...
DOI:
10.1111/j.1365-2761.2010.01234.x
PubMed:
21294751

4. A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the BVDV and BPIV3

Author:
Sen Yang; Qian-Ying Wang; Bin Tan; Peng-Fei Shi; Lian-Jiang Qiao; Zhi-Jie Li; Ke-Xin Liu; Zhi-Gang Cao; Shu-Qin Zhang; Fei-Yan Sun
Source:
Journal of virological methods 2022 v.299 pp. 114343
ISSN:
0166-0934
Subject:
Bovine alphaherpesvirus 1; Bovine respirovirus 3; Bovine viral diarrhea virus 1; Classical swine fever virus; RNA; ambient temperature; bovine respiratory disease; cattle; cross reaction; diagnostic techniques; feedlots; genes; phosphoproteins; recombinase polymerase amplification; China
Abstract:
... Bovine respiratory disease complex (BRDC) is a serious disease affecting feedlot cattle in China and likely other places worldwide. Bovine viral diarrhea virus (BVDV) and bovine parainfluenza virus type 3 (BPIV3) are principally responsible for causing BRDC, and are a major strain to the industrial economy. Eradication of these viruses/disease requires swift viral identification and treatment. Hen ...
DOI:
10.1016/j.jviromet.2021.114343

5. A loop-mediated isothermal amplification assay for the detection and quantification of JC polyomavirus in cerebrospinal fluid: a diagnostic and clinical management tool and technique for progressive multifocal leukoencephalopathy

Author:
Hitomi Kinoshita; Kazuo Nakamichi; Chang-Kweng Lim; Mutsuyo Takayama-Ito; Lixin Wang; Itoe Iizuka; Ichiro Kurane; Masayuki Saijo
Source:
Virology journal 2018 v.15 no.1 pp. 136
ISSN:
1743-422X
Subject:
quantitative polymerase chain reaction; cross reaction; Human polyomavirus 2; central nervous system; genome; prognosis; therapeutics; cerebrospinal fluid; plasmids; encephalitis; patients; loop-mediated isothermal amplification; RNA
Abstract:
... BACKGROUND: JC polyomavirus (JCV) is the causative agent of progressive multifocal leukoencephalopathy (PML), a demyelinating disease of the central nervous system in immunosuppressed patients. PML usually has a poor prognosis. Detection and quantification of the JCV genome in cerebrospinal fluid (CSF) is an efficacious tool for the diagnosis and management of PML, for which proper therapeutic int ...
DOI:
10.1186/s12985-018-1046-z
PubMed:
30170628
PubMed Central:
PMC6119251

6. A novel ViewRNA in situ hybridization method for the detection of the dynamic distribution of Classical Swine Fever Virus RNA in PK15 cells

Author:
Qianyi Zhang; Lu Xu; Yujie Zhang; Tuanjie Wang; Xingqi Zou; Yuanyuan Zhu; Yan Zhao; Cui Li; Kai Chen; Yongfang Sun; Junxiang Sun; Qizu Zhao; Qin Wang
Source:
Virology journal 2017 v.14 no.1 pp. 81
ISSN:
1743-422X
Subject:
Classical swine fever virus; Ungulate protoparvovirus 1; cross reaction; Suid alphaherpesvirus 1; detection limit; immunohistochemistry; fluorescence; viruses; cytoplasm; genotype; formalin; actin; virulence; hog cholera; in situ hybridization; cell nucleus; fluorescent antibody technique; swine; tissue culture; proteinases; cultured cells; Bovine viral diarrhea virus 1; essential genes; RNA; China
Abstract:
... BACKGROUND: Classical swine fever (CSF) is a highly contagious fatal infectious disease caused by classical swine fever virus (CSFV). A better understanding of CSFV replication is important for the study of pathogenic mechanism of CSF. With the development of novel RNA in situ Hybridization method, quantitatively localization and visualization of the virus RNA molecular in cultured cell or tissue ...
DOI:
10.1186/s12985-017-0734-4
PubMed:
28420390
PubMed Central:
PMC5395781

7. A novel method of real‐time reverse‐transcription loop‐mediated isothermal amplification developed for rapid and quantitative detection of a new genotype (YHV‐8) of yellow head virus

Author:
H.‐L. Yang; L. Qiu; Q. Liu; X.‐Y. Wan; S. Liu; L.‐L. Zhu; B. Yang; Q.‐L. Zhang; J. Huang
Source:
Letters in applied microbiology 2016 v.63 no.2 pp. 103-110
ISSN:
0266-8254
Subject:
Fenneropenaeus chinensis; Gill-associated virus; Litopenaeus vannamei; Macrobrachium rosenbergii; Marsupenaeus japonicus; Protoparvovirus; RNA; Taura syndrome virus; White spot syndrome virus; correlation; cross reaction; detection limit; farms; genome; genotype; magnesium; monitoring; mortality; necrosis; reverse transcription loop-mediated isothermal amplification; shrimp; specific pathogen-free animals; virulence; viruses; China
Abstract:
... A new genotype of yellow head virus (YHV), designated as YHV‐8, was found in farmed shrimp Fenneropenaeus chinensis suffering suspectedly from EMS/AHPNS (early mortality disease/acute hepatopancreatic necrosis disease) in China in 2012. In this study, a one‐step, real‐time reverse‐transcription loop‐mediated isothermal amplification (rRT‐LAMP) assay was developed for better detection of both genot ...
DOI:
10.1111/lam.12591

8. A one-step molecular biology method for simple and rapid detection of grass carp Ctenopharyngodon idella reovirus (GCRV) HZ08 strain

Author:
W. W. Zeng; Q. Wang; Y. Y. Wang; D. H. Xu; S. Q. Wu
Source:
Journal of fish biology 2013 v.82 no.5 pp. 1545-1555
ISSN:
1095-8649
Subject:
Ctenopharyngodon idella; DNA primers; RNA; Reoviridae; cross reaction; detection limit; fish; fluorescence; genes; loop-mediated isothermal amplification; molecular biology; reverse transcriptase polymerase chain reaction; reverse transcription; viruses
Abstract:
... Six reverse-transcription loop-mediated isothermal amplification (RT-LAMP) primers designed against conserved regions of segment 6 (s6) gene were used for the detection of grass carp Ctenopharyngodon idella reovirus (GCRV) HZ08 subtype. The entire amplification could be completed within 40 min at 62·3◦ C. The RT-LAMP showed higher sensitivity than reverse-transcription polymerase chain reaction (R ...
Handle:
10113/60501
DOI:
10.1111/jfb.12088
PubMed:
23639153

9. A one-step, one-pot CRISPR nucleic acid detection platform (CRISPR-top): Application for the diagnosis of COVID-19

Author:
Shijun Li; Junfei Huang; Lijuan Ren; Weijia Jiang; Ming Wang; Li Zhuang; Qinni Zheng; Rui Yang; Yi Zeng; Laurence Don Wai Luu; Yi Wang; Jun Tai
Source:
Talanta 2021 v.233 pp. 122591
ISSN:
0039-9140
Subject:
COVID-19 infection; DNA; RNA; Severe acute respiratory syndrome coronavirus 2; cross contamination; cross reaction; detection limit; etiological agents; fluorescence; nucleoproteins; oropharynx; risk; temperature
Abstract:
... The existing CRISPR-mediated diagnostic tests require a two-step procedure (DNA or RNA amplification followed by CRISPR-mediated sequence-specific detection) for nucleic acid detection, which increases complexity and the risk of sample cross-contamination. Here, we report a new CRISPR-mediated test, called CRISPR-top (CRISPR-mediated testing in one-pot), which integrates simultaneous target pre-am ...
DOI:
10.1016/j.talanta.2021.122591

10. A panel of real-time PCR assays for the detection of Bourbon virus, Heartland virus, West Nile virus, and Trypanosoma cruzi in major disease–transmitting vectors

Author:
Anushri Warang; Michael Zhang; Shuping Zhang; Zhenyu Shen
Source:
Journal of veterinary diagnostic investigation 2021 v.33 no.6 pp. 1115-1122
ISSN:
1943-4936
Subject:
DNA; RNA; Trypanosoma cruzi; West Nile virus; animal health; cross reaction; monitoring; pathogens; public health; quantitative polymerase chain reaction; reverse transcription; viruses
Abstract:
... Vector-borne pathogens, such as Bourbon virus (BRBV), Heartland virus (HRTV), West Nile virus (WNV), and Trypanosoma cruzi (TCZ) are a great threat to public health and animal health. We developed a panel of TaqMan real-time PCR assays for pathogen surveillance. PCR targets were selected based on nucleic acid sequences deposited in GenBank. Primers and probes were either designed de novo or select ...
DOI:
10.1177/10406387211039549

11. A pre- and during Pandemic Survey of Sars-Cov-2 Infection in Stray Colony and Shelter Cats from a High Endemic Area of Northern Italy

Author:
Eva Spada; Fabrizio Vitale; Federica Bruno; Germano Castelli; Stefano Reale; Roberta Perego; Luciana Baggiani; Daniela Proverbio
Source:
Viruses 2021 v.13 no.4 pp. -
ISSN:
1999-4915
Subject:
Anaplasma phagocytophilum; COVID-19 infection; Chlamydophila felis; Felid alphaherpesvirus 1; Feline calicivirus; Feline immunodeficiency virus; Feline parvovirus; Leishmania infantum; RNA; Rickettsia; Severe acute respiratory syndrome coronavirus 2; Toxoplasma gondii; blood serum; cats; cross reaction; disease susceptibility; enzymes; humans; nucleocapsid; pandemic; peritonitis; pharynx; reverse transcriptase polymerase chain reaction; seroprevalence; surveys; Italy
Abstract:
... Cats are susceptible to infection with severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2). Whilst a number of studies have been performed worldwide on owned cats, limited data are available on stray, colony or shelter cats. We investigated SARS-CoV-2 infection in a stray cat population before and during human outbreaks of SARS-CoV-2 in cities in the Lombardy region in northern Italy, a h ...
DOI:
10.3390/v13040618

12. A rapidly new-typed detection of norovirus based on F0F1-ATPase molecular motor biosensor

Author:
Zhuo Zhao; Jie Zhang; Mei-Ling Xu; Zhi-Peng Liu; Hua Wang; Ming Liu; Yan-Yan Yu; Li Sun; Hui Zhang; Hai-Yan Wu
Source:
Biotechnology and bioprocess engineering 2016 v.21 no.1 pp. 128-133
ISSN:
1226-8372
Subject:
H-transporting ATP synthase; Norovirus; RNA; biosensors; cross reaction; kinesin; rapid methods; reverse transcriptase polymerase chain reaction; viruses
Abstract:
... In order to adapt port rapid detection of food borne norovirus, presently we developed a new typed detection method based on F₀F₁-ATPase molecular motor biosensor. A specific probe was encompassed the conservative region of norovirus and F₀F₁-ATPase within chromatophore was constructed as a molecular motor biosensor through the “ε-subunit antibody-streptomycin-biotin-probe” system. Norovirus was c ...
DOI:
10.1007/s12257-015-0384-6
PubMed:
32218681
PubMed Central:
PMC7091097

13. A real-time RT-PCR assay for molecular identification and quantitation of feline morbillivirus RNA from biological specimens

Author:
Eliana De Luca; Paolo Emidio Crisi; Marco Di Domenico; Daniela Malatesta; Giacomo Vincifori; Morena Di Tommaso; Giovanni Di Guardo; Gabriella Di Francesco; Antonio Petrini; Giovanni Savini; Andrea Boari; Alessio Lorusso
Source:
Journal of virological methods 2018 v.258 pp. 24-28
ISSN:
0166-0934
Subject:
Morbillivirus; RNA; blood sampling; cats; cross reaction; diagnostic techniques; genes; immunohistochemistry; pathogens; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; tissues; urine; viral antigens
Abstract:
... The aim of this study was to develop a real-time RT-PCR to detect and quantitate feline morbillivirus (FeMV) RNA in biological samples. Primers and probe were targeted on a conserved region of FeMV P/V/C gene. To validate the assay with field samples, a total number of specimens of cats have been recruited including 264 urine and blood samples and compared with a generic RT-PCR targeting the L pro ...
DOI:
10.1016/j.jviromet.2018.05.002

14. A reverse transcription loop‐mediated isothermal amplification assay for the detection of strawberry mottle virus

Author:
Junda Ren; Jia Wang; Yadong Zhu; Chenggui Han; Qiaoxia Shang
Source:
Phytopathologische Zeitschrift 2021 v.169 no.5 pp. 295-302
ISSN:
0931-1785
Subject:
RNA; Strawberry mottle virus; agarose; betaine; coat proteins; cross reaction; electrophoresis; gels; gene amplification; genes; rapid methods; reverse transcription loop-mediated isothermal amplification; strawberries; temperature; viruses
Abstract:
... Strawberry mottle virus (SMoV) is one of the most important viruses infecting strawberry all over the world, seriously affecting the yield and quality of strawberry especially in the occasion of compound infection. Loop‐mediated isothermal amplification (LAMP) as a new method of nucleic acid amplification has the advantages of rapid detection, simple operation and high sensitivity. Therefore, four ...
DOI:
10.1111/jph.12985

15. A smartphone-based visual biosensor for CRISPR-Cas powered SARS-CoV-2 diagnostics

Author:
Long Ma; Lijuan Yin; Xiaoyan Li; Si Chen; Lei Peng; Guozhen Liu; Shengying Ye; Wenlu Zhang; Shuli Man
Source:
Biosensors & bioelectronics 2022 v.195 pp. 113646
ISSN:
0956-5663
Subject:
COVID-19 infection; CRISPR-Cas systems; Pseudoviridae; RNA; Severe acute respiratory syndrome coronavirus 2; biosensors; color; cross reaction; detection limit; diagnostic techniques; genes; mobile telephones; nanogold; pandemic; single-stranded DNA
Abstract:
... The pandemic of coronavirus disease 2019 (COVID-19) resulted from novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a worldwide concern. It is imperative to develop rapid, sensitive, and specific biosensing methods. Herein, we developed a CRISPR-Cas12a powered visual biosensor with a smartphone readout for ultrasensitive and selective detection of SARS-CoV-2. Simply, th ...
DOI:
10.1016/j.bios.2021.113646

16. A two-tube multiplex real-time RT-PCR assay for the detection of four hemorrhagic fever viruses: severe fever with thrombocytopenia syndrome virus, Hantaan virus, Seoul virus, and dengue virus

Author:
Zhifeng Li; Xian Qi; Minghao Zhou; Changjun Bao; Jianli Hu; Bin Wu; Shenjiao Wang; Zhongmin Tan; Jianguang Fu; Jun Shan; Yefei Zhu; Fenyang Tang
Source:
Archives of virology 2013 v.158 no.9 pp. 1857-1863
ISSN:
0304-8608
Subject:
screening; cross reaction; humans; hospitals; Seoul orthohantavirus; pathogens; viruses; Dengue virus; patients; Hantaan orthohantavirus; RNA; diagnostic sensitivity; fluorescent antibody technique; fever
Abstract:
... The aim of this study was to develop and evaluate a two-tube multiplex real-time RT-PCR assay for the detection and identification of four viral hemorrhagic fever (VHF) pathogens, severe fever with thrombocytopenia syndrome virus (SFTSV), Hantaan virus (HTNV), Seoul virus (SEOV), and dengue virus (DENV), from human clinical samples. The two-tube multiplex real-time RT-PCR assay we developed has a ...
DOI:
10.1007/s00705-013-1677-8
PubMed:
23532380

17. An encodable multiplex microsphere-phase amplification sensing platform detects SARS-CoV-2 mutations

Author:
Zecheng Zhong; Jin Wang; Shuizhen He; Xiaosong Su; Weida Huang; Mengyuan Chen; Zhihao Zhuo; Xiaomei Zhu; Mujin Fang; Tingdong Li; Shiyin Zhang; Shengxiang Ge; Jun Zhang; Ningshao Xia
Source:
Biosensors & bioelectronics 2022 v.203 pp. 114032
ISSN:
0956-5663
Subject:
COVID-19 infection; RNA; Severe acute respiratory syndrome coronavirus 2; biosensors; cross reaction; detection limit; diagnostic techniques; fluorescence; microparticles; mutation; pandemic; pathogenicity; pathogens; polymerase chain reaction
Abstract:
... SARS-CoV-2 variants of concern (VOCs) contain several single-nucleotide variants (SNVs) at key sites in the receptor-binding region (RBD) that enhance infectivity and transmission, as well as cause immune escape, resulting in an aggravation of the coronavirus disease 2019 (COVID-19) pandemic. Emerging VOCs have sparked the need for a diagnostic method capable of simultaneously monitoring these SNV ...
DOI:
10.1016/j.bios.2022.114032

18. An engineered anti-idiotypic antibody-derived killer peptide (KP) early activates swine inflammatory monocytes, CD3+CD16+ natural killer T cells and CD4+CD8α+ double positive CD8β+ cytotoxic T lymphocytes associated with TNF-α and IFN-γ secretion

Author:
Luca Ferrari; Paolo Martelli; Roberta Saleri; Elena De Angelis; Giulia Ferrarini; Valeria Cavalli; Benedetta Passeri; Gianluca Bazzoli; Giulia Ogno; Walter Magliani; Paolo Borghetti
Source:
Comparative immunology, microbiology, and infectious diseases 2020 v.72 pp. 101523
ISSN:
0147-9571
Subject:
Porcine circovirus-2; antibodies; cross reaction; cytotoxicity; dose response; flow cytometry; immunomodulation; immunomodulators; interferon-gamma; memory; monocytes; phenotype; porcine reproductive and respiratory syndrome; secretion; swine; toxins; tumor necrosis factor-alpha; vaccine adjuvants; viruses; yeasts
Abstract:
... This study evaluated the early modulation of the phenotype and cytokine secretion in swine immune cells treated with an engineered killer peptide (KP) based on an anti-idiotypic antibody functionally mimicking a yeast killer toxin. The influence of KP on specific immunity was investigated using porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) as ex v ...
DOI:
10.1016/j.cimid.2020.101523

19. Analysis of small RNAs derived from tomato yellow leaf curl Sardinia virus reveals a cross reaction between the major viral hotspot and the plant host genome

Author:
Laura Miozzi; Vitantonio Pantaleo; József Burgyán; Gian Paolo Accotto; Emanuela Noris
Source:
Virus research 2013 v.178 no.2 pp. 287-296
ISSN:
0168-1702
Subject:
RNA; RNA interference; Solanum lycopersicum var. lycopersicum; Tomato yellow leaf curl Sardinia virus; Tomato yellow leaf curl virus; bioinformatics; coat proteins; cross reaction; genes; high-throughput nucleotide sequencing; host plants; nucleotides; pathogens; ribonucleases; tomatoes; transcription (genetics); viruses; Mediterranean region
Abstract:
... RNA silencing is a defense mechanism exploited by plants against viruses. Upon infection, viral genomes and their transcripts are processed by Dicer-like (DCL) ribonucleases into viral small interfering RNAs (vsRNAs) of 21–24 nucleotides that further guide silencing of viral transcripts. To get an insight into the molecular interaction between tomato and the monopartite phloem-limited begomovirus ...
DOI:
10.1016/j.virusres.2013.09.029

20. Antigenic variation of foot and mouth disease virus - an overview

Author:
Longjam, Neeta; Tayo, Tilling
Source:
Veterinary world 2011 v.4 no.10 pp. 475-479
ISSN:
0972-8988
Subject:
Foot-and-mouth disease virus; RNA; amino acids; antigenic variation; cross reaction; foot-and-mouth disease; genetic recombination; livestock; mutation; serotypes; surface proteins; vaccines; viruses
Abstract:
... Foot and mouth disease (FMD) is a highly contagious viral diseases of cloven-hoofed animals, caused by FMD virus, a single molecule of linear positive sense, with single stranded RNA of size 7.2– 8.4 kb. Antigenic variation is one of the striking characters of FMD virus. It is a process by which an infectious organism alters its surface proteins in order to evade a host immune response and is asso ...

21. Application of cDNA Macroarray for Simultaneous Detection of 12 Potato Viruses

Author:
Maoka, T.; Sugiyama, S.; Maruta, Y.; Hataya, T.
Source:
Plant disease 2010 v.94 no.10 pp. 1248-1254
ISSN:
0191-2917
Subject:
plant viruses; cross reaction; diagnostic techniques; disease detection; complementary DNA; plant extracts; reverse transcriptase polymerase chain reaction; plasmids; pathogen identification; enzyme-linked immunosorbent assay; nucleotide sequences; RNA; nucleic acid hybridization; microbial genetics; disease diagnosis
Abstract:
... A complementary DNA (cDNA) macroarray was developed for simultaneous detection of 12 different potato viruses. A suitable region in the viral genome for each was selected for Alfalfa mosaic virus, Cucumber mosaic virus, Potato aucuba mosaic virus, Potato leafroll virus, Potato mop-top virus, Potato virus A, Potato virus M, Potato virus S, Potato virus X, Potato virus Y, Tomato ringspot virus, and ...
DOI:
10.1094/PDIS-12-09-0787

22. Aspergillus derived mycotoxins in food and the environment: Prevalence, detection, and toxicity

Author:
Vishwambar Navale; Koteswara Rao Vamkudoth; Shanthipriya Ajmera; Vaibhavi Dhuri
Source:
Toxicology reports 2021 v.8 pp. 1008-1030
ISSN:
2214-7500
Subject:
Aspergillus; RNA; aflatoxins; aflatrem; antibodies; bioaerosols; chemotypes; citrinin; cross reaction; cyclopiazonic acid; genomics; gliotoxins; human health; hypersensitivity; hyphae; ligands; metabolomics; mortality; ochratoxin A; oligonucleotides; patulin; point-of-care systems; proteomics; public health; risk; single-stranded DNA; sterigmatocystin; systematic evolution of ligands by exponential enrichment; toxicity; toxicology; transcriptomics; uncertainty
Abstract:
... Aspergillus species are the paramount ubiquitous fungi that contaminate various food substrates and produce biochemicals known as mycotoxins. Aflatoxins (AFTs), ochratoxin A (OTA), patulin (PAT), citrinin (CIT), aflatrem (AT), secalonic acids (SA), cyclopiazonic acid (CPA), terrein (TR), sterigmatocystin (ST) and gliotoxin (GT), and other toxins produced by species of Aspergillus plays a major rol ...
DOI:
10.1016/j.toxrep.2021.04.013

23. Assessment of cross-protection induced by a bluetongue virus (BTV) serotype 8 vaccine towards other BTV serotypes in experimental conditions

Author:
Ludovic Martinelle; Fabiana Dal Pozzo; Christine Thys; Ilse De Leeuw; Willem Van Campe; Kris De Clercq; Etienne Thiry; Claude Saegerman
Source:
Veterinary research 2018 v.49 no.1 pp. 63
ISSN:
1297-9716
Subject:
Bluetongue virus; Holstein; RNA; blood; bluetongue; calves; cross immunity; cross reaction; hosts; males; necropsy; neutralizing antibodies; serotypes; signs and symptoms (animals and humans); vaccination; vaccines; veterinary medicine; viruses; Europe
Abstract:
... Bluetongue disease is caused by bluetongue virus (BTV) and BTV serotype 8 (BTV8) caused great economic damage in Europe during the last decade. From 1998 to 2007, in addition to BTV8, Europe had to face the emergence of BTV1, 2, 4, 9, and 16, spreading in countries where the virus has never been detected before. These unprecedented outbreaks trigger the need to evaluate and compare the clinical, v ...
DOI:
10.1186/s13567-018-0556-4
PubMed:
30012223
PubMed Central:
PMC6048908

24. Avian paramyoxvirus-8 immunization reduces viral shedding after homologous APMV-8 challenge but fails to protect against Newcastle disease

Author:
Christian Grund; Constanze Steglich; Eva Huthmann; Martin Beer; Thomas C Mettenleiter; Angela Römer-Oberdörfer
Source:
Virology journal 2014 v.11 no.1 pp. 179
ISSN:
1743-422X
Subject:
chickens; cross reaction; Newcastle disease; specific pathogen-free animals; Orthorubulavirus; morbidity; Influenza A virus; vaccines; chimerism; Avian orthoavulavirus 1; hemagglutinins; mortality; viruses; immunization; viral shedding; virus replication; RNA; virulence; glycoproteins
Abstract:
... BACKGROUND: Protection against infection by Newcastle disease virus (NDV), also designated as avian paramyxovirus subtype-1 (APMV-1), is mediated by immune responses to the two surface glycoproteins, hemagglutinin-neuraminidase (HN) and fusion (F) protein. Thus, a chimeric APMV-1 based vaccine that encodes APMV-8 HN- and F-proteins and expresses the hemagglutinin of avian influenza virus (AIV) H5N ...
DOI:
10.1186/1743-422X-11-179
PubMed:
25297904
PubMed Central:
PMC4203933

25. Clinical evaluation of the automated Abbott RealTime SARS-CoV-2, Alinity m SARS-CoV-2, and Alinity m Resp-4-Plex assays

Author:
Robert Ehret; Stefan Breuer; Jens Dhein; Birgit Reinhardt; Martin Obermeier
Source:
Journal of virological methods 2022 v.299 pp. 114338
ISSN:
0166-0934
Subject:
RNA; Severe acute respiratory syndrome coronavirus 2; automation; clinical examination; cross reaction; influenza; throat
Abstract:
... Detection of SARS-CoV-2 infections relies on the use of sensitive, accurate and high throughput RT-PCR assays.We assessed the analytical performance of the Abbott RealTime SARS-CoV-2 (RT-SARS), Alinity m SARS-CoV-2 (AlinSARS) assays and compared the clinical performance of the RT-SARS, AlinSARS, and Alinity m Resp-4-Plex (Alin4Plex) assays to the Seegene Allplex assay (Allplex) and an inhouse test ...
DOI:
10.1016/j.jviromet.2021.114338

26. Cloning, Expression, and the Effects of Processing on Sarcoplasmic-Calcium-Binding Protein: An Important Allergen in Mud Crab

Author:
Meng-Jun Hu; Guang-Yu Liu; Yang Yang; Tzu-Ming Pan; Yi-Xiang Liu; Le-Chang Sun; Min-Jie Cao; Guang-Ming Liu
Source:
Journal of agricultural and food chemistry 2017 v.65 no.30 pp. 6247-6257
ISSN:
0021-8561
Subject:
Escherichia coli; Maillard reaction; RNA; Scylla paramamosain; allergenicity; allergens; amino acids; calcium-binding proteins; circular dichroism spectroscopy; complementary DNA; crabs; cross reaction; crosslinking; desorption; enzyme-linked immunosorbent assay; food processing; hypersensitivity; immunoglobulin E; mass spectrometry; muscles; open reading frames; patients; scanning electron microscopy; seafoods; shellfish
Abstract:
... Shellfish allergy is a prevalent, long-lasting disorder usually persisting throughout life. However, the allergen information is incomprehensive in crab. This study aimed to identify a novel allergen in crab, show its potential in diagnosis and reduce the allergenicity by food processing. A 21-kDa protein was purified from Scylla paramamosain and confirmed as sarcoplasmic calcium binding protein ( ...
DOI:
10.1021/acs.jafc.7b02381

27. Comparative Sequences of Canine and Feline Endothelin-1

Author:
Biondo, Alexander W.; Wiedmeyer, Charles E.; Sisson, David D.; Solter, Phillip F.
Source:
Veterinary clinical pathology 2003 v.32 no.4 pp. 188-194
ISSN:
0275-6382
Subject:
DNA; RNA; amino acid sequences; analytical kits; antibodies; blood; cardiovascular diseases; cats; cross reaction; diagnostic techniques; dogs; endothelins; humans; immunoassays; leucine; methionine; mice; rats; reverse transcriptase polymerase chain reaction
Abstract:
... Background: Endothelin-1 (ET-1, “mature ET-1”) is a potent vasoconstrictor peptide that is made along with “big ET-1” from its precursor, preproET-1. Increased plasma concentrations of ET-1 and big ET-1 occur with various forms of cardiovascular disease in humans. Our laboratory is investigating plasma endothelins as diagnostic tests of cardiovascular disease in dogs and cats; however, commercial ...
DOI:
10.1111/j.1939-165X.2003.tb00334.x

28. Comparative analyses of SARS-CoV-2 binding (IgG, IgM, IgA) and neutralizing antibodies from human serum samples

Author:
Livia Mazzini; Donata Martinuzzi; Inesa Hyseni; Linda Benincasa; Eleonora Molesti; Elisa Casa; Giulia Lapini; Pietro Piu; Claudia Maria Trombetta; Serena Marchi; Ilaria Razzano; Alessandro Manenti; Emanuele Montomoli
Source:
Journal of immunological methods 2021 v.489 pp. 112937
ISSN:
0022-1759
Subject:
COVID-19 infection; RNA; Severe acute respiratory syndrome coronavirus 2; biosecurity; blood serum; cross reaction; human population; humans; humoral immunity; immunogenicity; monitoring; neutralization; neutralization tests; viruses; China
Abstract:
... A newly identified coronavirus, named SARS-CoV-2, emerged in December 2019 in Hubei Province, China, and quickly spread throughout the world; so far, it has caused more than 49.7 million cases of disease and 1,2 million deaths. The diagnosis of SARS-CoV-2 infection is currently based on the detection of viral RNA in nasopharyngeal swabs by means of molecular-based assays, such as real-time RT-PCR. ...
DOI:
10.1016/j.jim.2020.112937

29. Comparison of four RT-PCR assays for detection of bovine respiratory syncytial virus

Author:
W. Socha; J. Rola
Source:
Polish journal of veterinary sciences 2011 v.14 no.3 pp. 449-451
ISSN:
1505-1773
Subject:
cross reaction; nucleoproteins; Bovine orthopneumovirus; reverse transcriptase polymerase chain reaction; viruses; genes; RNA; glycoproteins
Abstract:
... RT-PCR assays for detection of BRSV, based on four different sets of primers were optimized and evaluated for their sensitivity and specificity. Primers used in this study were specific for genes encoding three BRSV proteins, nucleoprotein N and glycoproteins F and G. Our results indicated that RT-PCR with primers B7:B8 for G protein was the most efficient in detecting BRSV. Starters B7:B8 reacted ...
DOI:
10.2478/v10181-011-0066-z

30. Contamination-free visual detection of SARS-CoV-2 with CRISPR/Cas12a: A promising method in the point-of-care detection

Author:
Yanju Chen; Ya Shi; Yin Chen; Zhangnv Yang; Hui Wu; Zhihui Zhou; Jue Li; Jianfeng Ping; Luping He; Hong Shen; Zhengxin Chen; Jian Wu; Yunsong Yu; Yanjun Zhang; Huan Chen
Source:
Biosensors & bioelectronics 2020 v.169 pp. 112642
ISSN:
0956-5663
Subject:
COVID-19 infection; Centers for Disease Control and Prevention; DNA; Orthomyxoviridae; RNA; Severe acute respiratory syndrome coronavirus 2; aerosols; biosensors; cross reaction; fluorescence; mobile telephones; point-of-care systems; China
Abstract:
... The outbreaks of the infectious disease COVID-19 caused by SARS-CoV-2 seriously threatened the life of humans. A rapid, reliable and specific detection method was urgently needed. Herein, we reported a contamination-free visual detection method for SARS-CoV-2 with LAMP and CRISPR/Cas12a technology. CRISPR/Cas12a reagents were pre-added on the inner wall of the tube lid. After LAMP reaction, CRISPR ...
DOI:
10.1016/j.bios.2020.112642

31. Dengue serotyping with a label-free DNA sensor

Author:
S. K. Chan; Y. S. Choong; D. Perera; T. S. Lim
Source:
Analytical methods 2018 v.10 no.2 pp. 214-222
ISSN:
1759-9679
Subject:
Culicidae; DNA; Dengue virus; RNA; analytical methods; arboviruses; cross reaction; dengue hemorrhagic fever; detection limit; fluorescence; human resources; nanosilver; nucleic acid hybridization; nucleotide sequences; serotypes; subtropics; ultraviolet radiation
Abstract:
... Dengue virus (DENV) is one of the most important mosquito-borne viruses in tropical and subtropical regions. Development of severe forms of dengue viral infection such as dengue fever (DF) and dengue hemorrhagic fever (DHF) has claimed many lives. The standard methods for detecting dengue virus are time consuming, laborious, and require skilful personnel. In this study, we propose a method whereby ...
DOI:
10.1039/c7ay02131c

32. Design and assessment of a real time reverse transcription-PCR method to genotype single-stranded RNA male-specific coliphages (Family Leviviridae)

Author:
Stephanie D. Friedman; Emilie M. Cooper; Kevin R. Calci; Fred J. Genthner
Source:
Journal of virological methods 2011 v.173 no.2 pp. 196-202
ISSN:
0166-0934
Subject:
Leviviridae; RNA; animal wastes; coliphages; cross reaction; databases; genotype; humans; nucleotide sequences; quality control; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; sewage
Abstract:
... A real-time, reverse transcription-PCR (RT-qPCR) assay was developed to differentiate the four genogroups of male-specific ssRNA coliphages (FRNA) (family Leviviridae). As FRNA display a trend of source-specificity (human sewage or animal waste) at the genogroup level, this assay provides a tool to help identify the origin of fecal contamination. Primers and probes were designed using complete gen ...
DOI:
10.1016/j.jviromet.2011.02.005

33. Detecting Infectious Bursal Disease Using a VP1 Gene-Based RT-qPCR Assay Compared to Standard Methods of Virus Isolation, ELISA, and Histopathology

Author:
Cintia H. Okino; Daiane Voss-Rech; Fátima R. F. Jaenisch; Iara M. Trevisol; Raquel Rebelatto; Arlei Coldebella; Marcos A. Z. Mores; Rodrigo Giglioti; Clarissa S. L. Vaz
Source:
Current microbiology 2020 v.77 no.6 pp. 1043-1050
ISSN:
0343-8651
Subject:
Infectious bursal disease virus; RNA; analytical specificity; cross reaction; detection limit; disease prevention; enzyme-linked immunosorbent assay; genes; histopathology; hydrolysis; immunosuppression; infectious bursal disease; monitoring; pathogens; poultry production; reverse transcriptase polymerase chain reaction; vaccination; viruses
Abstract:
... Infectious bursal disease (IBD) is an immunosuppressive viral disease of chickens, associated with severe economic losses and major threats to poultry production worldwide. Disease prevention programs rely on unequivocal identification of the pathogen, as well as vaccination programs. This study developed a sensitive, one-step, real-time, quantitative reverse transcription polymerase chain reactio ...
DOI:
10.1007/s00284-020-01906-7

34. Detection and characterization of hepatitis A virus in water samples in Thailand

Author:
Kittigul, L.; Uthaisin, A.; Ekchaloemkiet, S.; Utrarachkij, F.; Luksamijarulkul, P.
Source:
Journal of applied microbiology 2006 v.100 no.6 pp. 1318-1323
ISSN:
1364-5072
Subject:
water supply; hepatitis A; nucleotides; cross reaction; risk; detection limit; DNA; viruses; genotype; people; Escherichia coli; coliform bacteria; sequence analysis; drinking; polymerase chain reaction; tap water; swamps; rivers; Hepatovirus A; nucleotide sequences; RNA; containers; Thailand
Abstract:
... Outbreaks of hepatitis A in Thailand have been reported continuely and associated with water supply. However, the genetic analysis of hepatitis A virus (HAV) in water is limited. This study described the application of virus concentration method and reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) to detect HAV RNA and analyse the genetic sequence of the virus in environmenta ...
DOI:
10.1111/j.1365-2672.2006.02876.x

35. Detection and differentiation of respiratory syncytial virus subgroups A and B with colorimetric toehold switch sensors in a paper-based cell-free system

Author:
Mengcen Cao; Qiuli Sun; Xu Zhang; Yi Ma; Jufang Wang
Source:
Biosensors & bioelectronics 2021 v.182 pp. 113173
ISSN:
0956-5663
Subject:
Human coronavirus HKU1; RNA; Respiratory syncytial virus; Severe acute respiratory syndrome coronavirus 2; biosensors; cell free system; colorimetry; cross reaction; detection limit; genes; humans; infectious diseases; rapid methods
Abstract:
... Respiratory syncytial virus (RSV) infection is the most common clinical infectious disease threatening the safety of human life. Herein, we provided a sensitive and specific method for detection and differentiation of RSV subgroups A (RSVA) and B (RSVB) with colorimetric toehold switch sensors in a paper-based cell-free system. In this method, we applied the toehold switch, an RNA-based riboswitch ...
DOI:
10.1016/j.bios.2021.113173

36. Detection of Aichi virus with antibody targeting of conserved viral protein 1 epitope

Author:
Yao-Shen Chen; Bao-Chen Chen; You-Sheng Lin; Jenn-Tzong Chang; Tsi-Shu Huang; Jih-Jung Chen; Tsung-Hsien Chang
Source:
Applied microbiology and biotechnology 2013 v.97 no.19 pp. 8529-8536
ISSN:
0175-7598
Subject:
gastroenteritis; cross reaction; immunoblotting; antigens; humans; polyclonal antibodies; enzyme-linked immunosorbent assay; Kobuvirus; viral proteins; virology; viruses; neonates; RNA; fluorescent antibody technique; interferons; tropisms; Taiwan
Abstract:
... Aichi virus (AiV) is an emerging single-stranded, positive-sense, non-enveloped RNA virus in the Picornaviridae that causes acute gastroenteritis in humans. The first case of AiV infection in Taiwan was diagnosed in a human neonate with enterovirus-associated symptoms; the virus was successfully isolated and propagated. To establish a method to detect AiV, we analyzed the antigen epitope and gener ...
DOI:
10.1007/s00253-012-4644-5
PubMed:
23306637

37. Detection of Cattle Infected with Bovine Viral Diarrhea Virus Using Nucleic Acid Hybridization

Author:
Karen L. Roberts; James K. Collins; Jane Carman; Carol D. Blair
Source:
Journal of veterinary diagnostic investigation v.3 no.1 pp. 10-15
ISSN:
1040-6387
Subject:
Venezuelan equine encephalitis virus; cross reaction; Equine arteritis virus; Bluetongue virus; Japanese encephalitis virus; Bovine orthopneumovirus; cattle; cell culture; genome; leukocytes; viruses; Bovine viral diarrhea virus 1; border disease; Dengue virus; nucleotide sequences; RNA; nucleic acid hybridization; nylon
Abstract:
... A ribonucleic acid (RNA) hybridization assay to identify cattle infected by bovine viral diarrhea virus (BVDV) is described. The RNA probe was derived from the coding region at the 3′ end of the genome of the NADL strain of BVDV. Total RNA from infected cell cultures or peripheral blood leukocytes from suspect animals was extracted and applied to nylon membranes with a slot blot apparatus. Periphe ...
DOI:
10.1177/104063879100300103

38. Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Author:
Kazuya Shirato; Takuya Yano; Syouhei Senba; Shigehiro Akachi; Takashi Kobayashi; Takamichi Nishinaka; Tsugunori Notomi; Shutoku Matsuyama
Source:
Virology journal 2014 v.11 no.1 pp. 139
ISSN:
1743-422X
Subject:
DNA primers; Middle East respiratory syndrome coronavirus; RNA; World Health Organization; cross reaction; diagnostic techniques; disease diagnosis; humans; loop-mediated isothermal amplification; microbial detection; monitoring; nucleocapsid proteins; pathogens; reverse transcriptase polymerase chain reaction; reverse transcription; temperature; viruses; Saudi Arabia
Abstract:
... BACKGROUND: The first documented case of Middle East Respiratory Syndrome coronavirus (MERS-CoV) occurred in 2012, and outbreaks have continued ever since, mainly in Saudi Arabia. MERS-CoV is primarily diagnosed using a real-time RT-PCR assay, with at least two different genomic targets required for a positive diagnosis according to the case definition of The World Health Organization (WHO) as of ...
DOI:
10.1186/1743-422X-11-139
PubMed:
25103205
PubMed Central:
PMC4132226

39. Detection of SARS-CoV-2-specific antibodies via rapid diagnostic immunoassays in COVID-19 patients

Author:
Jira Chansaenroj; Ritthideach Yorsaeng; Nawarat Posuwan; Jiratchaya Puenpa; Natthinee Sudhinaraset; Chintana Chirathaworn; Yong Poovorawan
Source:
Virology journal 2021 v.18 no.1 pp. 52
ISSN:
1743-422X
Subject:
COVID-19 infection; RNA; Severe acute respiratory syndrome coronavirus 2; blood donors; blood serum; chemiluminescence; cross reaction; microparticles; nucleocapsid; reverse transcriptase polymerase chain reaction; seroprevalence; virology
Abstract:
... BACKGROUND: Efficient monitoring and control of coronavirus disease 2019 (COVID-19) require access to diagnostic tests, and serological diagnostic testing is desirable. In the current study, antibodies were investigated in patients recently diagnosed with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. METHODS: Cross-sectional data were obtained from 245 patients in whom SA ...
DOI:
10.1186/s12985-021-01530-2

40. Detection of swine transmissible gastroenteritis coronavirus using loop-mediated isothermal amplification

Author:
Qin Chen; Jian Li; Xue-En Fang; Wei Xiong
Source:
Virology journal 2010 v.7 no.1 pp. 206
ISSN:
1743-422X
Subject:
RNA; Transmissible gastroenteritis virus; cross reaction; detection limit; digestion; genes; loop-mediated isothermal amplification; nucleocapsid; transmissible gastroenteritis; virology
Abstract:
... A conserved nucleic acid fragment of the nucleocapsid gene of Swine Transmissible Gastroenteritis Coronavirus (TGEV) was chosen as the target, six special primers were designed successfully. Loop-mediated isothermal amplification (LAMP) was developed to detect the TGEV by incubation at 60°C for 1 h and the product specificity was confirmed by Hph I digestion. Standard curves with high accuracy for ...
DOI:
10.1186/1743-422X-7-206

41. Detection of the allergenic celery protein component (Api g 1.01) in foods by immunoassay

Author:
Haiyan Wang; Gang Li; Fei Yuan; Yajun Wu; Ying Chen
Source:
European food research & technology 2011 v.233 no.6 pp. 1023-1028
ISSN:
1438-2377
Subject:
G-proteins; RNA; adverse effects; allergens; carrots; celery; cross reaction; enzyme-linked immunosorbent assay; foods; hypersensitivity; monoclonal antibodies; protein sources; protein synthesis
Abstract:
... Among food allergens, celery is a frequent cause for adverse food reactions in allergic patients. In this study, the celery allergen protein Api g 1.01 RNA was amplified by RT-PCR and cloned into the pET-32a expression vector. The recombinant plasmid was transformed into E.coli BL21(DE3) pLys for the expression of protein Api g 1.01. Monoclonal antibodies were prepared against the expressed purifi ...
DOI:
10.1007/s00217-011-1597-3

42. Detection of the toxic marine diatom Pseudo-nitzschia multiseries using the RuBisCO small subunit (rbcS) gene in two real-time RNA amplification formats

Author:
Jennifer A. Delaney; Robert M. Ulrich; John H. Paul
Source:
Harmful algae 2011 v.11 no. pp. 54-64
ISSN:
1568-9883
Subject:
RNA; cross reaction; databases; detection limit; domoic acid; genes; humans; mortality; nucleotide sequences; photoperiod; reverse transcriptase polymerase chain reaction; ribulose-bisphosphate carboxylase; toxicity; wildlife
Abstract:
... The marine diatom genus Pseudo-nitzschia includes species that produce domoic acid, a neurotoxin responsible for illness and mortality in both humans and marine wildlife. Because of the expertise and time required for the microscopic discrimination of species, molecular methods that monitor environmental concentrations of Pseudo-nitzschia provide a rapid alternative for the early detection of bloo ...
DOI:
10.1016/j.hal.2011.07.005

43. Development and application of a loop-mediated isothermal amplification assay on rapid and sensitive detection of rotavirus in fecal samples and artificially seeded oysters

Author:
Xiaoxia Kou; Hongying Fan; Qingping Wu; Liang Xue; Jumei Zhang
Source:
Food control 2014 v.41 pp. 151-157
ISSN:
0956-7135
Subject:
RNA; Rotavirus; betaine; color; cross reaction; detection limit; feces; gel electrophoresis; genome; magnesium sulfate; monitoring; oysters; reverse transcription loop-mediated isothermal amplification; risk assessment; turbidity
Abstract:
... A reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of rotavirus. Four primers were designed to recognize six distinct regions based on a highly conserved sequence in the VP7 region of the rotavirus genome. The optimal conditions for the LAMP assay were determined to 62 °C for 60 min with 8.0 mM MgSO4, 0.2 M betaine, 0.8 mM dNTPs, 0.2 μM each ...
DOI:
10.1016/j.foodcont.2014.01.013

44. Development and evaluation of a broadly reactive reverse transcription recombinase polymerase amplification assay for rapid detection of murine norovirus

Author:
Lei Ma; Fanwen Zeng; Feng Cong; Bihong Huang; Yujun Zhu; Miaoli Wu; Fengjiao Xu; Wen Yuan; Ren Huang; Pengju Guo
Source:
BMC veterinary research 2018 v.14 no.1 pp. 399
ISSN:
1746-6148
Subject:
Norovirus; RNA; cross reaction; feces; mice; monitoring; mutants; pathogens; point mutation; quantitative polymerase chain reaction; rapid methods; reverse transcriptase polymerase chain reaction; reverse transcription; viruses
Abstract:
... BACKGROUND: Murine norovirus (MNV) is recognized as the most prevalent viral pathogen in captive mouse colonies. The rapid detection assay for MNV would be a useful tool for monitoring and preventing MNV infection. A recombinase polymerase amplification (RPA) assay was established in this study to provide a solution for rapid and sensitive detection of MNV. RESULTS: The detection limit of the RT-R ...
DOI:
10.1186/s12917-018-1736-1
PubMed:
30547776
PubMed Central:
PMC6295035

45. Development and evaluation of a real-time RT-PCR assay for detection of a novel avian influenza A (H5N6) virus

Author:
Rusheng Zhang; Dong Yao; Jingfang Chen; Wen Ye; Xinhua Ou; Tianmu Chen; Biancheng Sun
Source:
Journal of virological methods 2018 v.257 pp. 79-84
ISSN:
0166-0934
Subject:
Orthomyxoviridae; RNA; animal pathogens; avian influenza; birds; cross reaction; detection limit; genetic analysis; genome; hemagglutinins; human diseases; humans; markets; nucleotide sequences; oligodeoxyribonucleotides; patients; poultry; public health; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; risk; sialidase; throat; viruses; China
Abstract:
... As of Aug 25, 2017, 17 incidences of human infection and 6 deaths due to the novel H5N6 virus have been reported in China. Genetic analysis of the viral genome revealed that this reassortant virus is highly pathogenic to poultry, and that the virus has a risk of transmission to humans. Accordingly, the development of a rapid, sensitive, and specific molecular diagnostic assay is critical for publi ...
DOI:
10.1016/j.jviromet.2018.05.001

46. Development and evaluation of indirect double-antibody sandwich ELISA for rapid detection of Salmonid Alphavirus using Baculoviridae expressed E1 Protein

Author:
Shuai Gao; Na Wang; Jiawei Yang; Jinhui Sun; Yuting Wang; Dong Xia; Jingwen Tian; Yuntong Zhao; Ying Feng; Ying Zhou; Xueting Guan; Wen Shi; Min Liu
Source:
Aquaculture 2021 v.544 pp. 737081
ISSN:
0044-8486
Subject:
Alphavirus; Baculoviridae; Oncorhynchus mykiss; RNA; Salmo salar; cross reaction; enzyme-linked immunosorbent assay; epidemiology; fluorescent antibody technique; insects; mortality; pathogens; quantitative polymerase chain reaction; rapid methods; salmon; trade; viruses; Europe
Abstract:
... Salmon alphavirus (SAV) is a widespread virus that infects Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) in Europe. It has a fatality rate of ≤48% and can cause pancreatic and sleeping disease. With the frequent exchange of salmon farming trade, it may become a global pathogen; therefore, a sensitive, effective, and high-throughput detection method is necessary. The serolog ...
DOI:
10.1016/j.aquaculture.2021.737081

47. Development and performance evaluation of the first in-house multiplex rRT-PCR assay in Bangladesh for highly sensitive detection of SARS-CoV-2

Author:
Farjana Akther Noor; Kazi Sarjana Safain; Md. Walid Hossain; Khalid Arafath; Kaiissar Mannoor; Mazbahul Kabir
Source:
Journal of virological methods 2021 v.293 pp. 114147
ISSN:
0166-0934
Subject:
COVID-19 infection; RNA; Severe acute respiratory syndrome coronavirus 2; cost effectiveness; cross reaction; detection limit; equipment; genes; humans; nucleocapsid; pandemic; reverse transcriptase polymerase chain reaction; ribonucleases; Bangladesh
Abstract:
... The emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic is posing a great threat to global health and economy. Due to the lack of broad diagnostic setup, consistent reagent supply lines, and access to laboratory instruments and equipment, it is undoubtedly an enormous burden for developing countries to face the crisis. To develop a cost-effective, reliable and sensit ...
DOI:
10.1016/j.jviromet.2021.114147

48. Development and validation of sensitive real-time RT-PCR assay for broad detection of rabies virus

Author:
Martin Faye; Laurent Dacheux; Manfred Weidmann; Sylvie Audrey Diop; Cheikh Loucoubar; Hervé Bourhy; Amadou Alpha Sall; Ousmane Faye
Source:
Journal of virological methods 2017 pp. -
ISSN:
0166-0934
Subject:
quantitative polymerase chain reaction; cross reaction; nucleoproteins; detection limit; Rabies lyssavirus; monitoring; rabies; epizootic diseases; hosts; brain; reverse transcriptase polymerase chain reaction; viruses; encephalitis; RNA; pathogens
Abstract:
... Rabies virus (RABV) remains one of the most important global zoonotic pathogens. RABV causes rabies, an acute encephalomyelitis associated with a high rate of mortality in humans and animals and affecting different parts of the world, particularly in Asia and Africa. Confirmation of rabies diagnosis relies on laboratory diagnosis, in which molecular techniques such as detection of viral RNA by rev ...
DOI:
10.1016/j.jviromet.2016.12.019

49. Development of A Super-Sensitive Diagnostic Method for African Swine Fever Using CRISPR Techniques

Author:
Meishen Ren; Hong Mei; Ming Zhou; Zhen F. Fu; Heyou Han; Dingren Bi; Fuhu Peng; Ling Zhao
Source:
Virologica Sinica 2021 v.36 no.2 pp. 220-230
ISSN:
1674-0769
Subject:
African swine fever; African swine fever virus; RNA; cross reaction; diagnostic techniques; fever; fluorescence; food security; mortality; plasmids; pork industry; quantitative polymerase chain reaction; quarantine; recombinase polymerase amplification; swine
Abstract:
... African swine fever (ASF) is an infectious disease caused by African swine fever virus (ASFV) with clinical symptoms of high fever, hemorrhages and high mortality rate, posing a threat to the global swine industry and food security. Quarantine and control of ASFV is crucial for preventing swine industry from ASFV infection. In this study, a recombinase polymerase amplification (RPA)-CRISPR-based n ...
DOI:
10.1007/s12250-020-00323-1

50. Development of RNA aptamers for detection of Salmonella Enteritidis

Author:
Ji-Yeon Hyeon; Jung-Whan Chon; In-Soo Choi; Chankyu Park; Dong-Eun Kim; Kun-Ho Seo
Source:
Journal of microbiological methods 2012 v.89 no.1 pp. 79-82
ISSN:
0167-7012
Subject:
RNA; Salmonella Enteritidis; cross reaction; oligonucleotides; screening; serotypes
Abstract:
... We developed and evaluated RNA aptamers to analyze their potential for use in detecting Salmonella Enteritidis. The selected aptamer was observed to specifically bind to Salmonella Enteritidis without any cross-reactivity to other Salmonella serovars. Thus, this study suggests that aptamers specific to Salmonella Enteritidis have a high potential for use in presumptive presumptive screening method ...
DOI:
10.1016/j.mimet.2012.01.014