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You searched for: "Xue, Liang" Remove constraint "Xue, Liang" Subject term RNA Remove constraint Subject term: RNA
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1. LION: a simple and rapid method to achieve CRISPR gene editing

Author:
Xue Liang,   et al.   ; Xi Xiang; Lidan Luo; Michał Nodzyński; Conghui Li; Peng Han; Hongwei Dou; Trine Skov Petersen; Xiaoguang Pan; Kunli Qu; Ling Yang; Yonghui Dang; Xin Liu; Lars Bolund; Xiuqing Zhang; Guangdong Tong; Yufeng Xing; Yonglun Luo; Lin Lin;     Show all 19 Authors
Source:
Cellular and molecular life sciences 2019 v.76 no.13 pp. 2633-2645
ISSN:
1420-682X
Subject:
CRISPR-Cas systems; Felidae; RNA; cost effectiveness; gene editing; gene targeting; humans; plasmids; rapid methods
Abstract:
... The RNA-guided CRISPR–Cas9 technology has paved the way for rapid and cost-effective gene editing. However, there is still a great need for effective methods for rapid generation and validation of CRISPR/Cas9 gRNAs. Previously, we have demonstrated that highly efficient generation of multiplexed CRISPR guide RNA (gRNA) expression array can be achieved with Golden Gate Assembly (GGA). Here, we pres ...
DOI:
10.1007/s00018-019-03064-x

2. Preliminary serological evidence for Schmallenberg virus infection in China

Author:
Xue-Liang Zhu,   et al.   ; Shao-Lun Zhai; Dian-Hong Lv; Xiao-Hui Wen; Yan-Qiu Yang; Qin-Ling Chen; Wen-Kang Wei;     Show all 7 Authors
Source:
Tropical animal health and production 2018 v.50 no.2 pp. 449-453
ISSN:
0049-4747
Subject:
quantitative polymerase chain reaction; buffaloes; blood serum; antibodies; goats; subtropics; Schmallenberg orthobunyavirus; arboviruses; dairy cattle; enzyme-linked immunosorbent assay; pathogenesis; RNA; West Asia; Africa; Europe; China
Abstract:
... Schmallenberg virus (SBV) is an emerging and rampant arbovirus in Europe, and even Africa and West Asia. Investigating whether SBV existed in new regions or countries, it was very helpful for the early warning and control of SBV. In this study, we collected 317 serum samples (n = 242 for dairy cattle, n = 13 for yellow cattle, n = 21 for buffalo, and n = 41 for goats) from Guangdong province of so ...
DOI:
10.1007/s11250-017-1433-2