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You searched for: Subject term Rotavirus Remove constraint Subject term: Rotavirus Subject term RNA Remove constraint Subject term: RNA
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1. Comparative Analysis of Public RNA-Sequencing Data from Human Intestinal Enteroid (HIEs) Infected with Enteric RNA Viruses Identifies Universal and Virus-Specific Epithelial Responses

Author:
Roberto J. Cieza; Jonathan L. Golob; Justin A. Colacino; Christiane E. Wobus
Source:
Viruses 2021 v.13 no.6 pp. -
ISSN:
1999-4915
Subject:
Astroviridae; Norovirus; RNA; Rotavirus; biomimetics; biopsy; burden of disease; data collection; gastroenteritis; humans; interferons; intestinal mucosa; organoids; sequence analysis; transcription (genetics); viruses
Abstract:
... Acute gastroenteritis (AGE) has a significant disease burden on society. Noroviruses, rotaviruses, and astroviruses are important viral causes of AGE but are relatively understudied enteric pathogens. Recent developments in novel biomimetic human models of enteric disease are opening new possibilities for studying human-specific host–microbe interactions. Human intestinal enteroids (HIE), which ar ...
DOI:
10.3390/v13061059

2. Genomic analysis of group A rotavirus G12P[8] including a new Japanese strain revealed evidence for intergenotypic recombination in VP7 and VP4 genes

Author:
Tung Phan; Tomihiko Ide; Satoshi Komoto; Pattara Khamrin; Ngan Thi Kim Pham; Shoko Okitsu; Koki Taniguchi; Shuichi Nishimura; Niwat Maneekarn; Satoshi Hayakawa; Hiroshi Ushijima
Source:
Infection, genetics, and evolution 2021 v.87 pp. 104656
ISSN:
1567-1348
Subject:
Japan; RNA; Rotavirus A; evolution; gastroenteritis; genes; genetic analysis; humans; infection; metagenomics; swine
Abstract:
... Group A rotavirus is a leading cause of severe acute gastroenteritis worldwide. In this study, the first complete coding sequences of 11 RNA segments of human group A rotavirus G12P[8] in Japan were determined by an unbiased viral metagenomics. Its genomic constellation (VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5 genes) was identified as G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1. When performing t ...
DOI:
10.1016/j.meegid.2020.104656

3. Genetic and Antigenic Characterization and Retrospective Surveillance of Bovine Influenza D Viruses Identified in Hokkaido, Japan from 2018 to 2020

Author:
Jun Hayakawa; Tomomi Masuko; Tae Takehana; Tohru Suzuki
Source:
Viruses 2020 v.12 no.8 pp. -
ISSN:
1999-4915
Subject:
Influenza D virus; RNA; Rotavirus; cattle; cell culture; cross reaction; genotype; monitoring; neutralization tests; phylogeny; respiratory tract diseases; viral antigens; Japan
Abstract:
... Influenza D virus (IDV), which is a new member of the Orthomyxoviridae family, is potentially involved in bovine respiratory diseases (BRDs). Bovine IDVs (BIDVs) from Japan have been distributed nationwide since 2010 and are genetically distinct from foreign IDVs. We isolated BIDVs from three BRD outbreaks, in Hokkaido during 2018–2020, to understand their genetic and antigenic characteristics. Re ...
DOI:
10.3390/v12080877

4. Porcine Astrovirus Type 5-Associated Enteritis in Pigs

Author:
Tanja Opriessnig; Chao-Ting Xiao; Patrick G. Halbur
Source:
Journal of comparative pathology 2020 v.181 pp. 38-46
ISSN:
0021-9975
Subject:
Mamastrovirus 3; RNA; Rotavirus; antigens; biosafety; enteritis; enterocytes; genes; genomics; necropsy; neonates; pathogenesis
Abstract:
... Astroviruses (AstVs) are emerging, potentially zoonotic pathogens, associated with enteric and neurological signs in various animal species. In pigs, five lineages have been identified, porcine AstVs (PoAstVs) 1–5. We now report PoAstV5-associated atrophic enteritis in a colostrum-deprived (CD) pig and a similar disease in field cases of enteritis. Four newborn CD piglets were housed under biosafe ...
DOI:
10.1016/j.jcpa.2020.09.014

5. Prevalence and molecular characterization of parechovirus A in children with acute gastroenteritis in Shenzhen, 2016–2018

Author:
Ya-Nan Zhu; Yu-Hui Ye; Zhen Zhang; Yan-Jie Wu; Long Chen; Jing Wang; Yi-Jun Tang; Jun Meng; Hai-Long Zhang; Gui-Fang Hu
Source:
Archives of virology 2020 v.165 no.6 pp. 1377-1384
ISSN:
0304-8608
Subject:
Adenoviridae; Parechovirus A; RNA; Rotavirus; gastroenteritis; genetic variation; genotype; genotyping; mixed infection; molecular epidemiology; phylogeny; summer; virology; viruses; China
Abstract:
... Parechovirus A (PeV-A), which causes a wide variety of diseases, is prevalent among young children. However, little is currently known about PeV-A infections in children with acute gastroenteritis in mainland China. In this study, we investigated the molecular epidemiology of acute gastroenteritis in Shenzhen, southern China, with an emphasis on PeV-A infections. A total of 1220 stool specimens fr ...
DOI:
10.1007/s00705-020-04587-6

6. Whole genome sequence of an uncommon G9P[4] species A rotavirus containing DS-1-like (genotype 2) genes in Japan

Author:
Tung Phan; Riona Hatazawa; Satoshi Komoto; Shuichi Nishimura; Pattara Khamrin; Ngan Thi Kim Pham; Shoko Okitsu; Takashi Kobayashi; Niwat Maneekarn; Satoshi Hayakawa; Hiroshi Ushijima
Source:
Archives of virology 2022 v.167 no.7 pp. 1603-1606
ISSN:
0304-8608
Subject:
Japan; RNA; Rotavirus; children; diarrhea; feces; gastroenteritis; genomics; genotype; nucleotide sequences; virology
Abstract:
... Species A rotaviruses (RVAs) have been recognized as one of the leading causes of acute gastroenteritis in humans worldwide. Here, the complete coding sequences of 11 RNA segments of an uncommon G9P[4] RVA strain, which was detected in feces of a diarrheal child in Japan, were determined by next-generation sequencing technology. Its genomic constellation, VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP ...
DOI:
10.1007/s00705-022-05462-2

7. Epidemiology and genetic diversity of group A rotavirus in acute diarrhea patients in pre-vaccination era in Himachal Pradesh, India

Author:
Shipra Gupta; Sanjeev Chaudhary; Parvesh Bubber; Pratima Ray
Source:
Vaccine 2019 v.37 no.36 pp. 5350-5356
ISSN:
0264-410X
Subject:
RNA; Rotavirus A; children; developing countries; diarrhea; epidemiology; feces; gastroenteritis; genetic variation; genomics; genotype; morbidity; mortality; patients; reverse transcriptase polymerase chain reaction; strain differences; vaccination; vaccines; vomiting; India
Abstract:
... Acute gastroenteritis due to Group A rotaviruses remains the leading cause of mortality and morbidity in children in developing countries. India introduced its indigenous rotavirus vaccine Rotavac® in 2016 and Himachal Pradesh (HP) the first state to launch it. The present study aimed to evaluate rotavirus strain diversity associated with AGE prior to vaccine introduction in HP. A total of 331 fec ...
DOI:
10.1016/j.vaccine.2019.07.037

8. Experimental Methods to Study the Pathogenesis of Human Enteric RNA Viruses

Author:
Somya Aggarwal; Ebrahim Hassan; Megan T. Baldridge
Source:
Viruses 2021 v.13 no.6 pp. -
ISSN:
1999-4915
Subject:
Norovirus; RNA; Rotavirus; diarrhea; digestive tract; gastroenteritis; humans; mice; pathogenesis
Abstract:
... Every year, millions of children are infected with viruses that target the gastrointestinal tract, causing acute gastroenteritis and diarrheal illness. Indeed, approximately 700 million episodes of diarrhea occur in children under five annually, with RNA viruses norovirus, rotavirus, and astrovirus serving as major causative pathogens. Numerous methodological advancements in recent years, includin ...
DOI:
10.3390/v13060975

9. Free Chlorine Disinfection Mechanisms of Rotaviruses and Human Norovirus Surrogate Tulane Virus Attached to Fresh Produce Surfaces

Author:
Miyu Fuzawa; Elbashir Araud; Jianrong Li; Joanna L. Shisler; Thanh H. Nguyen
Source:
Environmental science & technology 2019 v.53 no.20 pp. 11999-12006
ISSN:
1520-5851
Subject:
Norovirus; RNA; Rotavirus; Tulane virus; capsid; chlorine; disinfection; environmental science; fresh produce; humans; kale; viral genome; viruses
Abstract:
... To fill the knowledge gap on how effective free chlorine is against viral-contaminated produce, we inoculated the surfaces of outdoor- or greenhouse-grown kale and mustard with Rotavirus (RV) or a human norovirus surrogate (Tulane virus, TV) and then disinfected the leaves with free chlorine. Disinfection efficacies for RV strain OSU and Wa were approximately 1-log₁₀ higher when attached to mustar ...
DOI:
10.1021/acs.est.9b03461
CHORUS:
10.1021/acs.est.9b03461

10. Genetic diversity and molecular epidemiological characterization of group A rotaviruses in raw sewage in Jinan by next generation sequencing

Author:
Haibo Du; Ping Xiong; Feng Ji; Xiaojuan Lin; Suting Wang; Li Zhang; Yao Liu; Yanyan Song; Zexin Tao; Aiqiang Xu
Source:
Infection, genetics, and evolution 2021 v.91 pp. 104814
ISSN:
1567-1348
Subject:
RNA; Rotavirus A; autumn; environmental monitoring; genetic variation; infection; molecular epidemiology; phylogeny; sewage; winter
Abstract:
... To understand the genetic diversity and molecular epidemiology characteristics of group A Rotavirus (RVA) in domestic sewage through next generation sequencing (NGS), and to explore the feasibility and necessity of NGS method for RVA environmental surveillance. In this study, two sewage samples from Jinan each quarter in 2019 were selected for concentration, RNA extraction, and then RT-PCR reactio ...
DOI:
10.1016/j.meegid.2021.104814

11. Persistence of murine norovirus, bovine rotavirus, and hepatitis A virus on stainless steel surfaces, in spring water, and on blueberries

Author:
Danielle Leblanc; Marie-Josée Gagné; Élyse Poitras; Julie Brassard
Source:
Food microbiology 2019 v.84 pp. 103257
ISSN:
0740-0020
Subject:
Hepatovirus A; Norovirus; RNA; Rotavirus; blueberries; bottled water; capsid; control methods; pathogenicity; ribonucleases; stainless steel; temperature; viability; viruses
Abstract:
... The viability of murine norovirus (MNV-1), bovine rotavirus (boRV), and hepatitis A virus (HAV) was evaluated at 21 °C, 4 °C, and −20 °C on stainless steel surfaces, in bottled water, and on blueberries for up to 21 days. After 14 days of incubation at 21 °C on stainless steel, a viability loss >4 log for MNV-1, >8 log for boRV, and >1 log for HAV was observed. Losses were observed for MNV-1 (>1 l ...
DOI:
10.1016/j.fm.2019.103257

12. Runting and Stunting Syndrome in Broiler Chickens: Histopathology and Association With a Novel Picornavirus

Author:
Letícia B. de Oliveira; James B. Stanton; Jian Zhang; Corrie Brown; Salman Latif Butt; Kiril Dimitrov; Claudio L. Afonso; Jeremy D. Volkening; Leonardo J. C. Lara; Camila Stefanie F. de Oliveira; Roselene Ecco
Source:
Veterinary pathology 2021 v.58 no.1 pp. 123-135
ISSN:
1544-2217
Subject:
Avian nephritis virus; RNA; Rotavirus; animal pathology; atrophy; epidemiology; epithelium; etiology; genes; genetic similarity; histology; histopathology; hybridization; ileum; jejunum; pathogenesis; serosa
Abstract:
... Runting stunting syndrome (RSS) in commercial chickens has been reported worldwide, and although several studies have attempted to clarify the cause and describe the lesions, there are gaps in knowledge of the epidemiology, pathogenesis, and etiology. The study objective was to use commercial chicks naturally affected by RSS to describe the histologic changes of RSS in all segments of the small in ...
DOI:
10.1177/0300985820969971

13. Understanding the Central Nervous System Symptoms of Rotavirus: A Qualitative Review

Author:
Arash Hellysaz; Marie Hagbom
Source:
Viruses 2021 v.13 no.4 pp. -
ISSN:
1999-4915
Subject:
RNA; Rotavirus; blood-brain barrier; brain; cytokines; diarrhea; encephalitis; encephalopathy; enterotoxins; fever; intestines; pathogens; serotonin
Abstract:
... This qualitative review on rotavirus infection and its complications in the central nervous system (CNS) aims to understand the gut–brain mechanisms that give rise to CNS driven symptoms such as vomiting, fever, feelings of sickness, convulsions, encephalitis, and encephalopathy. There is substantial evidence to indicate the involvement of the gut–brain axis in symptoms such as vomiting and diarrh ...
DOI:
10.3390/v13040658

14. Virological Characterization of Roof-Harvested Rainwater of Densely Urbanized Low-Income Region

Author:
Tatsuo Shubo; Adriana Maranhão; Fernando César Ferreira; Tulio Machado Fumian; Márcia Maria Araújo Pimenta; Cláudia do Rosário Vaz Morgado; Simon Toze; Warish Ahmed; Jatinder Sidhu; Marize Pereira Miagostovich
Source:
Food and environmental virology 2021 v.13 no.3 pp. 412-420
ISSN:
1867-0334
Subject:
DNA; Mastadenovirus; Norovirus; Polyomaviridae; RNA; Rotavirus; birds; flocculation; genome; genotype; human health; nucleotide sequences; rain; skim milk; urbanization; virology; viruses; Brazil
Abstract:
... Roof-harvested rainwater (RHRW) is considered relatively clean water, even though the possible presence of pathogens in the water may pose human health risks. In this study, we investigated the occurrence of enteric viruses in the first flush (10 mm) of RHRW from a densely populated and low-income urbanized region of Rio de Janeiro. One hundred samples (5 L) were collected from 10 rainfall events ...
DOI:
10.1007/s12560-021-09484-y

15. A Comprehensive Review on Human Aichi Virus

Author:
Enrique Rivadulla; Jesús L. Romalde
Source:
Virologica Sinica 2020 v.35 no.5 pp. 501-516
ISSN:
1674-0769
Subject:
Japan; Kobuvirus; RNA; Rotavirus; gastroenteritis; genome; human health; humans; nucleotides; pathogens; seroprevalence; viruses; France; Germany; Spain; Tunisia
Abstract:
... Although norovirus, rotavirus, adenovirus and Astrovirus are considered the most important viral agents transmitted by food and water, in recent years other viruses, such as Aichi virus (AiV), have emerged as responsible for gastroenteritis outbreaks associated with different foods. AiV belongs to the genus Kobuvirus of the family Picornaviridae. It is a virus with icosahedral morphology that pres ...
DOI:
10.1007/s12250-020-00222-5

16. Equine-like G3 rotavirus strains as predominant strains among children in Indonesia in 2015–2016

Author:
Takako Utsumi; Rury Mega Wahyuni; Yen Hai Doan; Zayyin Dinana; Soegeng Soegijanto; Yoshiki Fujii; Juniastuti; Laura Navika Yamani; Chieko Matsui; Lin Deng; Takayuki Abe; Soetjipto; Maria Inge Lusida; Koji Ishii; Hiroyuki Shimizu; Kazuhiko Katayama; Ikuo Shoji
Source:
Infection, genetics, and evolution 2018 v.61 pp. 224-228
ISSN:
1567-1348
Subject:
RNA; Rotavirus A; antigens; children; feces; females; gastroenteritis; genes; genotype; high-throughput nucleotide sequencing; hospitals; humans; immunoaffinity chromatography; males; patients; phylogeny; polyacrylamide gel electrophoresis; Australia; Brazil; Hungary; Indonesia; Spain
Abstract:
... Rotavirus A (RVA) is a major cause of acute gastroenteritis in humans and animals worldwide. As a result of the segmented nature of the rotavirus genome, genetic reassortment commonly occurs. This study aims to clarify the genetic characteristics of RVAs circulating in Indonesia. From June 2015 through August 2016, stool samples were collected from 134 children aged <5 years (71 male and 63 female ...
DOI:
10.1016/j.meegid.2018.03.027

17. Human viral pathogens are pervasive in wastewater treatment center aerosols

Author:
Evelyne Brisebois; Marc Veillette; Vanessa Dion-Dupont; Jacques Lavoie; Jacques Corbeil; Alexander Culley; Caroline Duchaine
Source:
Journal of environmental sciences (China) 2018 v.67 pp. 45-53
ISSN:
1001-0742
Subject:
Human alphaherpesvirus 1; Mastadenovirus; RNA; Rotavirus; aerosols; common cold; influenza; metagenomics; microbial communities; nucleotide sequences; pathogens; samplers; viruses; wastewater; wastewater treatment
Abstract:
... Wastewater treatment center (WTC) workers may be vulnerable to diseases caused by viruses, such as the common cold, influenza and gastro-intestinal infections. Although there is a substantial body of literature characterizing the microbial community found in wastewater, only a few studies have characterized the viral component of WTC aerosols, despite the fact that most diseases affecting WTC work ...
DOI:
10.1016/j.jes.2017.07.015
PubMed:
29778173
PubMed Central:
PMC7128102

18. Long-term monitoring of G1P[8] rotaviruses circulating without vaccine pressure in Nizhny Novgorod, Russia, 1984-2019

Author:
N. A. Novikova; T. A. Sashina; N. V. Epifanova; A. U. Kashnikov; O. V. Morozova
Source:
Archives of virology 2020 v.165 no.4 pp. 865-875
ISSN:
0304-8608
Subject:
RNA; Rotavirus; Russia; genes; genotype; genotyping; phylogeny; polyacrylamide gel electrophoresis; vaccines; virology
Abstract:
... The G1P[8] genotype is one of the most common among rotaviruses circulating in the last 40 years. Therefore, this genotype is a component of rotavirus vaccines licensed throughout the world. This paper presents the results of a 35-year (1984–2019) observation of the circulation of G1P[8] rotaviruses among children under 14 in one region (Nizhny Novgorod, Russia) without vaccine pressure. Several c ...
DOI:
10.1007/s00705-020-04553-2

19. Metagenomic Analysis of the Enteric RNA Virome of Infants from the Oukasie Clinic, North West Province, South Africa, Reveals Diverse Eukaryotic Viruses

Author:
Milton T. Mogotsi; Peter N. Mwangi; Phillip A. Bester; M. Jeffrey Mphahlele; Mapaseka L. Seheri; Hester G. O’Neill; Martin M. Nyaga
Source:
Viruses 2020 v.12 no.11 pp. -
ISSN:
1999-4915
Subject:
Astroviridae; Enterovirus C; Norovirus; Parechovirus; RNA; Rotavirus; community structure; digestive system; edible vaccines; feces; humans; infants; intestinal microorganisms; live vaccines; longitudinal studies; metagenomics; sample size; viral shedding; viruses; South Africa
Abstract:
... Establishing a diverse gut microbiota after birth is essential for preventing illnesses later in life. However, little knowledge exists about the total viral population (virome) present in the gut of infants during the early developmental stage, with RNA viruses being generally overlooked. Therefore, this small pilot longitudinal study investigated the diversity and changes in the enteric RNA viro ...
DOI:
10.3390/v12111260

20. Characterization of Norovirus and Other Human Enteric Viruses in Sewage and Stool Samples Through Next-Generation Sequencing

Author:
Sofia Strubbia; My V. T. Phan; Julien Schaeffer; Marion Koopmans; Matthew Cotten; Françoise S. Le Guyader
Source:
Food and environmental virology 2019 v.11 no.4 pp. 400-409
ISSN:
1867-0334
Subject:
Enterovirus; Norovirus; RNA; Rotavirus; Sapovirus; binding properties; capsid; feces; genome; high-throughput nucleotide sequencing; humans; molecular epidemiology; polyethylene glycol; sewage
Abstract:
... This study aimed to optimize a method to identify human enteric viruses in sewage and stool samples using random primed next-generation sequencing. We tested three methods, two employed virus enrichment based on the binding properties of the viral capsid using pig-mucin capture or by selecting viral RNA prior to library preparation through a capture using the SureSelect target enrichment. The thir ...
DOI:
10.1007/s12560-019-09402-3
PubMed:
31446609
PubMed Central:
PMC6848244

21. Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine

Author:
Sylvia F. S. Guerra; Priscylla C. M. S. Fecury; Delana A. M. Bezerra; Patricia S. Lobo; Edvaldo T. Penha Júnior; Edivaldo C. Sousa Júnior; Joana D’Arc P. Mascarenhas; Luana S. Soares; Maria Cleonice A. Justino; Alexandre C. Linhares
Source:
Archives of virology 2019 v.164 no.8 pp. 2107-2117
ISSN:
0304-8608
Subject:
RNA; Rotavirus; children; feces; gastroenteritis; genotype; infants; monitoring; reverse transcriptase polymerase chain reaction; sequence analysis; vaccines; Brazil
Abstract:
... Species A rotavirus still remains a major cause of acute gastroenteritis in infants and young children. Globally, six genotypes (G1P[8], G2P[4], G3P[8], G4P[8], G9P[8] and G12P[8]) account for >90% of circulating strains; however, genotype G12 in combination with P[6] or P[9] has been detected at increasing rates. We sought to broaden our knowledge about the rotavirus strains circulating during th ...
DOI:
10.1007/s00705-019-04295-w

22. In situ hybridization detection and subtyping of rotaviruses in swine samples

Author:
Talita P. Resende; Douglas Marthaler; Fabio A. Vannucci
Source:
Journal of veterinary diagnostic investigation 2019 v.31 no.1 pp. 113-117
ISSN:
1943-4936
Subject:
RNA; Rotavirus; antibodies; enteritis; immunohistochemistry; in situ hybridization; pathogenesis; reverse transcriptase polymerase chain reaction; swine; swine diseases; viruses
Abstract:
... Rotavirus groups A, B, and C (RVA, RVB, and RVC, respectively) have been the most prevalent and pathogenic in pigs. To date, immunohistochemistry is only available for RVA because of the lack of commercial antibodies for RVB and RVC. We developed a novel in situ hybridization RNA-based chromogenic technique (ISH-RNA) to detect and subtype RVA, RVB, and RVC. We evaluated 33 samples that were revers ...
DOI:
10.1177/1040638718817502
PubMed:
30541408
PubMed Central:
PMC6505766

23. The actin cytoskeleton is important for rotavirus internalization and RNA genome replication

Author:
Oscar Trejo-Cerro; Nayeli Aguilar-Hernández; Daniela Silva-Ayala; Susana López; Carlos F. Arias
Source:
Virus research 2019 v.263 pp. 27-33
ISSN:
0168-1702
Subject:
RNA; Rotavirus; genome; guanosinetriphosphatase; microfilament proteins; microfilaments; signal transduction; viruses
Abstract:
... Numerous host factors are required for the efficient replication of rotavirus, including the activation and inactivation of several cell signaling pathways. One of the cellular structures that are reorganized during rotavirus infection is the actin cytoskeleton. In this work, we report that the dynamics of the actin microfilaments are important at different stages of the virus life cycle, specific ...
DOI:
10.1016/j.virusres.2019.01.003
PubMed:
30639190
PubMed Central:
PMC7173133

24. The prevalence of enteric RNA viruses in stools from diarrheic and non-diarrheic people in southwestern Alberta, Canada

Author:
Danielle Leblanc; G. Douglas Inglis; Valerie F. Boras; Julie Brassard; Alain Houde
Source:
Archives of virology 2017 v.162 no.1 pp. 117-128
ISSN:
0304-8608
Subject:
Norovirus; RNA; Rotavirus; Sapovirus; children; diarrhea; elderly; enteritis; gastroenteritis; humans; livestock; people; sequence analysis; viruses; Alberta
Abstract:
... Southwestern Alberta is a region of Canada that has high rates of enteritis as well as high densities of livestock. The presence of enteric RNA viruses, specifically norovirus (NoV) GI, GII, GIII, GIV; sapovirus (SaV); rotavirus (RV); and astrovirus (AstV), was evaluated in stools from diarrheic (n = 2281) and non-diarrheic (n = 173) people over a 1-year period in 2008 and 2009. Diarrheic individu ...
DOI:
10.1007/s00705-016-3063-9

25. An integrated cell absorption process and quantitative PCR assay for the detection of the infectious virus in water

Author:
Xuan Guo; Shang Wang; Chong-lin Zhao; Jun-wen Li; Jin-yi Zhong
Source:
Science of the total environment 2018 v.635 pp. 964-971
ISSN:
0048-9697
Subject:
Adenoviridae; DNA; Enterovirus C; RNA; Rotavirus; absorption; cell culture; quantitative polymerase chain reaction; reverse transcription; river water; rivers; viruses
Abstract:
... Here we developed an integrated cell absorption process and quantitative (reverse transcription) polymerase chain reaction (ICAP-q(RT)PCR) assay to detect infectious viruses, which based on the detection of the viral nucleic acid (RNA or DNA) in the early stage of viral attachment and entry towards cells. The results showed that the poliovirus or adenovirus whose concentration was as low as 0.2 TC ...
DOI:
10.1016/j.scitotenv.2018.04.223

26. Detection of mammalian-like group a rotavirus in diarrhoeic poultry using RNA-PAGE In Kerala, India

Author:
Rinsha Balan; M. Mini; P.M Priya; Siju Joseph; Surya Sankar
Source:
Indian journal of animal research 2018 v.52 no.11 pp. 1654-1655
ISSN:
0976-0555
Subject:
RNA; Rotavirus A; animal research; birds; diarrhea; polyacrylamide gel electrophoresis; poultry; viruses; India
Abstract:
... The present work was conducted to determine the prevalence of rotavirus infection among poultry birds having diarrhoea. A total of 143 faecal samples were collected from different parts of Kerala and screened for the presence of rotavirus using RNA polyacrylamide gel electrophoresis (RNA-PAGE). Out of 143 samples, 5 (3.49%) were found to be positive in RNA PAGE with a migration pattern 4: 2: 3: 2 ...
DOI:
10.18805/ijar. B-3415

27. Magnetic bead-based nucleic acid purification kit: Clinical application and performance evaluation in stool specimens

Author:
Jihoon G. Yoon; Jin Seok Kang; Seung Yong Hwang; Jaewoo Song; Seok Hoon Jeong
Source:
Journal of microbiological methods 2016 v.124 pp. 62-68
ISSN:
0167-7012
Subject:
Clostridium difficile; DNA; RNA; analytical kits; automation; detection limit; feces
Abstract:
... Two different methods – the semi-automated magnetic bead-based kit (SK, Stool DNA/RNA Purification kit®) and the manual membrane column-based kit (QS, QIAamp® DNA Stool Mini kit) – for purifying nucleic acids from clinical stool samples were compared and evaluated. The SK kit was more user-friendly than QS due to the reduced manual processing, partial automation, and short turnaround time with hal ...
DOI:
10.1016/j.mimet.2016.03.011

28. Use of rapid needs assessment as a tool to identify vaccination delays in Guatemala and Peru

Author:
Katie K. D’Ardenne; Juliana Darrow; Anna Furniss; Catia Chavez; Herminio Hernandez; Stephen Berman; Edwin J. Asturias
Source:
Vaccine 2016 v.34 no.14 pp. 1719-1725
ISSN:
0264-410X
Subject:
RNA; children; educational status; gender; households; needs assessment; risk factors; surveys; vaccination; vaccines; Guatemala; Peru
Abstract:
... To explore the use of rapid needs assessment (RNA) surveys to determine the prevalence and factors contributing to delays in vaccination of children in two low middle-income countries (LMIC).Data from two RNA surveys performed as part of program improvement evaluations in Guatemala and Peru were used for this analysis. The primary endpoint was the timeliness of immunization with delay defined as a ...
DOI:
10.1016/j.vaccine.2016.01.060

29. Changing molecular epidemiology of rotavirus infection after introduction of monovalent rotavirus vaccination in Scotland

Author:
Indrani Mukhopadhya; Heather Murdoch; Susan Berry; Alison Hunt; Miren Iturriza-Gomara; Alison Smith-Palmer; J. Claire Cameron; Georgina L. Hold
Source:
Vaccine 2017 v.35 no.1 pp. 156-163
ISSN:
0264-410X
Subject:
RNA; Rotavirus; chi-square distribution; childhood; children; cross immunity; feces; gastroenteritis; genes; genotyping; molecular epidemiology; monitoring; phylogeny; polymerase chain reaction; vaccination; vaccines; virology; Scotland
Abstract:
... Rotaviruses (RV) are the leading cause of gastroenteritis in children less than five years of age worldwide. Rotarix®, a live attenuated monovalent vaccine containing a RV strain of G1P[8] specificity has been included in the childhood immunisation schedule from June 2013 in Scotland. This study aimed to characterise the prevalent RV strains in Scotland before and after the introduction of the RV ...
DOI:
10.1016/j.vaccine.2016.11.028

30. Colocalization of intracellular specific IgA (icIgA) with influenza virus in patients’ nasopharyngeal aspirate cells

Author:
Tuck-Weng Kok; Maurizio Costabile; Gregory A. Tannock; Peng Li
Source:
Journal of virological methods 2017 pp. -
ISSN:
0166-0934
Subject:
Influenza A virus; RNA; Rotavirus; antibodies; antiserum; epithelial cells; fluorescence microscopy; guinea pigs; hemagglutinins; immunization; immunoglobulin A; in vitro studies; influenza; mice; mucosal immunity; neutralization; patients; peptides; staining; vaccines; virion; virus replication; viruses
Abstract:
... Inhibition of viral replication by icIgA antibodies has only been observed with in vitro studies using epithelial cell lines in transwell cultures. This effect appears to involve an interaction between polymeric immunoglobulin A (pIgA) and viral particles within an intracellular compartment, since IgA is transported across polarized cells. Polyclonal guinea pig antisera against purified influenza ...
DOI:
10.1016/j.jviromet.2017.10.022

31. Evaluation of Virus Reduction by Ultrafiltration with Coagulation–Sedimentation in Water Reclamation

Author:
Suntae Lee; Akihiko Hata; Naoyuki Yamashita; Hiroaki Tanaka
Source:
Food and environmental virology 2017 v.9 no.4 pp. 453-463
ISSN:
1867-0334
Subject:
Rotavirus; quantitative polymerase chain reaction; risk; water reuse; detection limit; humans; risk management; t-test; ultrafiltration; models; bacteriophages; feces; Kobuvirus; Norovirus; RNA; Pepper mild mottle virus; Ryukyu Archipelago
Abstract:
... The evaluation of virus reduction in water reclamation processes is essential for proper assessment and management of the risk of infection by enteric viruses. Ultrafiltration (UF) with coagulation–sedimentation (CS) is potentially effective for efficient virus removal. However, its performance at removing indigenous viruses has not been evaluated. In this study, we evaluated the reduction of indi ...
DOI:
10.1007/s12560-017-9301-9

32. Evaluation of real-time RT-PCR assays for detection and quantification of norovirus genogroups I and II

Author:
Kitwadee Rupprom; Porntip Chavalitshewinkoon-Petmitr; Pornphan Diraphat; Leera Kittigul
Source:
Virologica Sinica 2017 v.32 no.2 pp. 139-146
ISSN:
1674-0769
Subject:
DNA; Hepatovirus A; Norovirus; RNA; Rotavirus; cross reaction; detection limit; gastroenteritis; reverse transcriptase polymerase chain reaction
Abstract:
... Noroviruses are the leading cause of acute gastroenteritis in humans. Real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) is a promising molecular method for the detection of noroviruses. In this study, the performance of three TaqMan real-time RT-PCR assays was assessed, which were one commercially available real-time RT-PCR kit (assay A: Norovirus Real Time RT-PCR kit) a ...
DOI:
10.1007/s12250-016-3863-9

33. Increased detection of G3P[9] and G6P[9] rotavirus strains in hospitalized children with acute diarrhea in Bulgaria

Author:
Zornitsa Mladenova; Sameena Nawaz; Balasubramanian Ganesh; Miren Iturriza-Gomara
Source:
Infection, genetics, and evolution 2015 v.29 pp. 118-126
ISSN:
1567-1348
Subject:
RNA; Rotavirus; birds; children; diarrhea; disease transmission; genome; human population; humans; monitoring; morbidity; mortality; pathogens; phylogeny; sequence analysis; vaccination; vaccines; Bulgaria
Abstract:
... Rotavirus severe disease in children is now vaccine-preventable and the roll-out of vaccination programs globally is expected to make a significant impact in the reduction of morbidity and mortality in children <5years of age. Rotavirus is also a pathogen of other mammals and birds, and its segmented RNA genome can lead to the emergence of new or unusual strains in human population via interspecie ...
DOI:
10.1016/j.meegid.2014.11.011

34. Molecular detection of enteric viruses from diarrheic calves in Egypt

Author:
Fakry F. Mohamed; Shimaa M. G. Mansour; Iman E. El-Araby; Sunil K. Mor; Sagar M. Goyal
Source:
Archives of virology 2017 v.162 no.1 pp. 129-137
ISSN:
0304-8608
Subject:
Rotavirus; Coronavirinae; phylogeny; diarrhea; farms; financial economics; feces; viruses; genotype; epidemiological studies; morbidity; transcription (genetics); control methods; Norovirus; calves; Bovine astrovirus; mortality; Torovirus; reverse transcriptase polymerase chain reaction; Bovine viral diarrhea virus 1; nucleotide sequences; RNA; sequence homology; Egypt
Abstract:
... Neonatal calf diarrhea (NCD) is a major cause of morbidity, mortality and economic losses in the beef and dairy industries. This study was conducted to investigate the existence of enteric viruses in two Egyptian farms with a history of recurrent diarrhea. Fecal samples were collected from 25 diarrheic calves. RNA was extracted and tested by reverse transcription polymerase chain reaction (RT-PCR) ...
DOI:
10.1007/s00705-016-3088-0
PubMed:
27686074
PubMed Central:
PMC7086814

35. Detection of the first G6P[14] human rotavirus strain in an infant with diarrhoea in Ghana

Author:
Susan Damanka; Belinda Lartey; Chantal Agbemabiese; Francis E. Dennis; Theophilus Adiku; Kofi Nyarko; Michael Ofori; George E. Armah
Source:
Virology journal 2016 v.13 no.1 pp. 183
ISSN:
1743-422X
Subject:
DNA; RNA; Rotavirus; amino acid sequences; amino acids; cattle; computer software; diarrhea; gastroenteritis; genes; genotype; humans; models; monitoring; nucleotide sequences; phylogeny; reverse transcriptase polymerase chain reaction; sequence analysis; vaccines; Belgium; France; Ghana; Italy; Spain
Abstract:
... BACKGROUND: Rotaviruses with G6P[14] specificity are mostly isolated in cattle and have been established as a rare cause of gastroenteritis in humans. This study reports the first detection of G6P[14] rotavirus strain in Ghana from the stool of an infant during a hospital-based rotavirus surveillance study in 2010. METHODS: Viral RNA was extracted and rotavirus VP7 and VP4 genes amplified by one s ...
DOI:
10.1186/s12985-016-0643-y
PubMed:
27832798
PubMed Central:
PMC5103419

36. Development of a quantitative real-time PCR assay for sapovirus in children under 5-years-old in Regina Margherita Hospital of Turin, Italy

Author:
Massimiliano Bergallo; Ilaria Galliano; Paola Montanari; Martina Rosa Brusin; Serena Finotti; Giulia Paderi; Clara Gabiano
Source:
Canadian journal of microbiology 2016 v.63 no.4 pp. 296-302
ISSN:
1480-3275
Subject:
DNA; Norovirus; RNA; Rotavirus; Sapovirus; children; diarrhea; feces; females; fever; gastroenteritis; genome; hospitals; humans; males; pain; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; transcription (genetics); viral load; vomiting; Italy
Abstract:
... Gastroenteritis is a common disease in children. It is characterized by diarrhea, vomiting, abdominal pain, and fever. Sapovirus (SaV) is a causative agent of acute gastroenteritis, but it causes milder illness than do rotavirus and norovirus. There is high variability in the analytical performance of quantitative PCR-based assays among clinical laboratories. This study developed a reverse transcr ...
DOI:
10.1139/cjm-2016-0482

37. Farm-level prevalence and risk factors for detection of hepatitis E virus, porcine enteric calicivirus, and rotavirus in Canadian finisher pigs

Author:
Barbara Wilhelm; Danielle Leblanc; David Leger; Sheryl Gow; Anne Deckert; David L. Pearl; Robert Friendship; Andrijana Raji; Alian Houde; Scott McEwen
Source:
Canadian journal of veterinary research 2016 v.80 no.2 pp. 95-105
ISSN:
0830-9000
Subject:
Rotavirus; pork; biosecurity; farm management; Orthohepevirus A; Porcine enteric calicivirus; farms; feces; viruses; feeder pigs; humans; herds; risk factors; FoodNet (CDC); Norovirus; quantitative polymerase chain reaction; questionnaires; antibiotic resistance; reverse transcriptase polymerase chain reaction; regression analysis; RNA; Canada
Abstract:
... Hepatitis E virus (HEV), norovirus (NoV), and rotavirus (RV) are all hypothesized to infect humans zoonotically via exposure through swine and pork. Our study objectives were to estimate Canadian farm-level prevalence of HEV, NoV [specifically porcine enteric calicivirus (PEC)], and RV in finisher pigs, and to study risk factors for farm level viral detection. Farms were recruited using the Canadi ...

38. Identification of OP354-like human rotavirus strains with subtype P[8]b in Ghanaian children with diarrhoea

Author:
Susan Damanka; Francis E. Dennis; Chantal Agbemabiese; Belinda Lartey; Theophilus Adiku; Kofi Nyarko; Christabel C. Enweronu-Laryea; Kwamena W. Sagoe; Michael Ofori; Onike Rodrigues; George E. Armah
Source:
Virology journal 2016 v.13 no.1 pp. 69
ISSN:
1743-422X
Subject:
RNA; Rotavirus; World Health Organization; algorithms; children; complementary DNA; computer software; diagnostic techniques; diarrhea; genes; genetic databases; genotype; genotyping; humans; models; nucleotide sequences; phylogeny; reverse transcriptase polymerase chain reaction; Ghana
Abstract:
... BACKGROUND: Rotaviruses with the P[8] genotype have been associated with majority of infections. Recent improvements in molecular diagnostics have delineated the P[8] genotype into P[8]a and P[8]b subtypes. P[8]a is the previously known P[8] genotype which is common whilst P[8]b subtype also known as OP354-like strain is genetically distinct, rarely detected and reported from a few countries. In a ...
DOI:
10.1186/s12985-016-0523-5
PubMed:
27103227
PubMed Central:
PMC4841057

39. Metagenomic analysis of viruses associated with field-grown and retail lettuce identifies human and animal viruses

Author:
Tiong Gim Aw; Samantha Wengert; Joan B. Rose
Source:
International journal of food microbiology 2016 v.223 pp. 50-56
ISSN:
0168-1605
Subject:
DNA viruses; Picobirnavirus; RNA; Rotavirus; bacteria; bioinformatics; community structure; farm to fork; food safety; fresh produce; genetic variation; high-throughput nucleotide sequencing; hosts; humans; lettuce; metagenomics; microbial communities; microbial detection; microbiome; safety testing; vertebrate viruses; viruses
Abstract:
... The emergence of culture- and sequence-independent metagenomic methods has not only provided great insight into the microbial community structure in a wide range of clinical and environmental samples but has also proven to be powerful tools for pathogen detection. Recent studies of the food microbiome have revealed the vast genetic diversity of bacteria associated with fresh produce. However, no w ...
DOI:
10.1016/j.ijfoodmicro.2016.02.008
CHORUS:
10.1016/j.ijfoodmicro.2016.02.008
Chorus Open Access:
10.1016/j.ijfoodmicro.2016.02.008

40. Molecular epidemiology of rotavirus in children and animals and characterization of an unusual G10P[15] strain associated with bovine diarrhea in south India

Author:
Priya Rajendran; Gagandeep Kang
Source:
Vaccine 2014 v.32 pp. A89
ISSN:
0264-410X
Subject:
RNA; Rotavirus A; birds; burden of disease; children; cows; dairy farming; data collection; diarrhea; disease transmission; enteropathogens; feces; genotype; genotyping; goats; hospitals; hosts; human diseases; humans; mixed infection; molecular epidemiology; reverse transcriptase polymerase chain reaction; vaccines; veterinary clinics; India
Abstract:
... Rotaviruses are enteric pathogens causing acute, watery, dehydrating diarrhea in various host species, including birds and mammals. This study collected data on the disease burden and strain prevalence of Group A rotavirus in animals and humans in Vellore and investigated interspecies transmission by comparison of circulating genotypes. Stool samples from children aged less than 5 years, admitted ...
DOI:
10.1016/j.vaccine.2014.03.026

41. Numbers of coliforms, Escherichia coli, F-RNA phage, rotavirus, bovine enteric calicivirus and presence of non-O157 STEC on commercial vacuum packaged beef

Author:
T.H. Jones; F.M. Nattress; B. Dilts; D. Olsen; V. Muehlhauser
Source:
Food microbiology 2014 v.42 pp. 225-231
ISSN:
0740-0020
Subject:
RNA; Rotavirus; Shiga toxin-producing Escherichia coli; Vesivirus; beef; cattle; coliform bacteria; coliphages; humans; pathogenicity; risk; vacuum packaging
Abstract:
... The numbers of coliforms, Escherichia coli, F-RNA coliphages, bovine enteric calicivirus (BEC) and rotavirus (RV) and presence of non-O157 shiga toxigenic E. coli (STEC) were determined on commercial vacuum packaged beef subprimals at the retail level from swabs obtained from the entire surfaces of 150 cuts that originated from federally and provincially registered plants. The prevalence and log m ...
DOI:
10.1016/j.fm.2014.04.001

42. PolyAdenine cryogels for fast and effective RNA purification

Author:
Kazım Köse; Kadir Erol; ErdoĿan ÿzgür; Lokman Uzun; Adil Denizli
Source:
Colloids and surfaces 2016 v.146 pp. 678-686
ISSN:
0927-7765
Subject:
Fourier transform infrared spectroscopy; RNA; adenine; adsorption; batch systems; colloids; denaturation; gene expression; pH; scanning electron microscopy; surface area; temperature; thermogravimetry; translation (genetics)
Abstract:
... Cryogels are used effectively for many diverse applications in a variety of fields. The isolation or purification of RNA, one of the potential utilizations for cryogels, is crucial due to their vital roles such as encoding, decoding, transcription and translation, and gene expression. RNA principally exists within every living thing, but their tendency to denaturation easily is still the most chal ...
DOI:
10.1016/j.colsurfb.2016.07.003

43. Sensitive and specific nested PCR assay for detection of rotavirus A in samples with a low viral load

Author:
Slavica Mijatovic-Rustempasic; Mathew D. Esona; Alice L. Williams; Michael D. Bowen
Source:
Journal of virological methods 2016 v.236 pp. 41-46
ISSN:
0166-0934
Subject:
RNA; Rotavirus A; feces; genotype; genotyping; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; viral load; viruses
Abstract:
... Techniques such as the real-time reverse transcription-polymerase chain reaction (qRT-PCR) can detect RNA in samples with a low viral load. However, these amplicons typically are either too short or at insufficient concentrations for use in subsequent sequencing reactions for genotyping and detection confirmation. The assay developed in this study detects rotavirus G genotypes and P genotypes with ...
DOI:
10.1016/j.jviromet.2016.07.007
PubMed:
27421626
PubMed Central:
PMC5075964

44. Comparative detection of rotavirus RNA by conventional RT-PCR, TaqMan RT-PCR and real-time nucleic acid sequence-based amplification

Author:
Qiu-Hua Mo; Hai-Bo Wang; Hua Tan; Bi-Mei Wu; Zi-Li Feng; Qi Wang; Ji-Can Lin; Ze Yang
Source:
Journal of virological methods 2015 v.213 pp. 1-4
ISSN:
0166-0934
Subject:
RNA; Rotavirus; detection limit; diarrhea; feces; molecular biology; pathogens; reverse transcriptase polymerase chain reaction; viruses
Abstract:
... Rotavirus is one of the major viral pathogens leading to diarrhea. Diagnosis has been conducted by either traditional cultural, serological methods or molecular biology techniques, which include RT-PCR and nucleic acid sequence-based amplification (NASBA). However, their differences regarding accuracy and sensitivity remain unknown. In this study, an in-house conventional RT-PCR assay and more imp ...
DOI:
10.1016/j.jviromet.2014.11.006

45. Comparative evaluation of commercially available manual and automated nucleic acid extraction methods for rotavirus RNA detection in stools

Author:
Mathew D. Esona; Sharla McDonald; Shifaq Kamili; Tara Kerin; Rashi Gautam; Michael D. Bowen
Source:
Journal of virological methods 2013 v.194 no.1-2 pp. 242-249
ISSN:
0166-0934
Subject:
RNA; Rotavirus; absorbance; children; enzyme immunoassays; feces; gastroenteritis; genotyping; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction
Abstract:
... Rotaviruses are a major cause of viral gastroenteritis in children. For accurate and sensitive detection of rotavirus RNA from stool samples by reverse transcription-polymerase chain reaction (RT-PCR), the extraction process must be robust. However, some extraction methods may not remove the strong RT-PCR inhibitors known to be present in stool samples. The objective of this study was to evaluate ...
DOI:
10.1016/j.jviromet.2013.08.023
PubMed:
24036075
PubMed Central:
PMC4603280

46. Detection and quantification of classic and emerging viruses by skimmed-milk flocculation and PCR in river water from two geographical areas

Author:
Byron Calgua; Tulio Fumian; Marta Rusiñol; Jesus Rodriguez-Manzano; Viviana A. Mbayed; Silvia Bofill-Mas; Marize Miagostovich; Rosina Girones
Source:
Water research 2013 v.47 pp. 2797-2810
ISSN:
0043-1354
Subject:
Adenoviridae; Polyomaviridae; Rotavirus; cost effectiveness; flocculation; freshwater; gastroenteritis; humans; quantitative polymerase chain reaction; river water; rivers; seawater; urban areas; vertebrate viruses; viruses; water quality; Brazil; Spain
Abstract:
... Molecular techniques and virus concentration methods have shown that previously unknown viruses are shed by humans and animals, and may be transmitted by sewage-contaminated water. In the present study, 10-L river-water samples from urban areas in Barcelona, Spain and Rio Janeiro, Brazil, have been analyzed to evaluate the viral dissemination of human viruses, validating also a low-cost concentrat ...
DOI:
10.1016/j.watres.2013.02.043
PubMed:
23535378

47. First report of a feather loss condition in Adelie penguins (Pygoscelis adeliae) on Ross Island, Antarctica, and a preliminary investigation of its cause

Author:
Wray W. Grimaldi; Richard J. Hall; Daniel D. White; Jing Wang; Melanie Massaro; Daniel M. Tompkins
Source:
Emu 2015 v.115 no.2 pp. 185-189
ISSN:
1448-5540
Subject:
Astroviridae; Beak and feather disease virus; Picornaviridae; Pygoscelis; RNA; Rotavirus; adults; alopecia; blood; ectoparasites; feathers; high-throughput nucleotide sequencing; molting; penguins; polymerase chain reaction; scanning electron microscopy; viruses; Antarctica
Abstract:
... Since the mid-1980s, observations of marine animals with alopecia have been reported worldwide, although in most cases specific causes and consequences have not been elucidated. Adding to that list, an unprecedented feather loss condition affecting ∼1 in 1000 adult Adelie penguins was observed at the beginning of December 2011 at each of three colonies on Ross Island, Antarctica. Feather loss was ...
DOI:
10.1071/MU14068

48. H2 genotypes of G4P[6], G5P[7], and G9[23] porcine rotaviruses show super-short RNA electropherotypes

Author:
Makoto Nagai; Saya Shimada; Yoshiki Fujii; Hiromitsu Moriyama; Mami Oba; Yukie Katayama; Shinobu Tsuchiaka; Sachiko Okazaki; Tsutomu Omatsu; Tetsuya Furuya; Satoshi Koyama; Junsuke Shirai; Kazuhiko Katayama; Tetsuya Mizutani
Source:
Veterinary microbiology 2015 v.176 no.3-4 pp. 250-256
ISSN:
0378-1135
Subject:
RNA; Rotavirus A; diarrhea; disease transmission; genes; genotype; humans; livestock and meat industry; monitoring; nucleotides; phylogeny; polyacrylamide gel electrophoresis; reverse transcriptase polymerase chain reaction; sequence analysis; swine; Japan
Abstract:
... During group A rotavirus (RVA) surveillance of pig farms in Japan, we detected three RVA strains (G4P[6], G5P[7], and G9P[23] genotypes), which showed super-short RNA patterns by polyacrylamide gel electrophoresis, in samples from a healthy eight-day-old pig and two pigs of seven and eight days old with diarrhea from three farms. Reverse transcription PCR and sequencing revealed that the full-leng ...
DOI:
10.1016/j.vetmic.2015.02.002

49. Multiplexed one-step RT-PCR VP7 and VP4 genotyping assays for rotaviruses using updated primers

Author:
Mathew D. Esona; Rashi Gautam; Ka Ian Tam; Alice Williams; Slavica Mijatovic-Rustempasic; Michael D. Bowen
Source:
Journal of virological methods 2015 v.223 pp. 96-104
ISSN:
0166-0934
Subject:
RNA; Rotavirus; antigens; detection limit; gastroenteritis; genotype; genotyping; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; viruses
Abstract:
... The current two-step VP7 and VP4 genotyping RT-PCR assays for rotaviruses have been linked consistently to genotyping failure in an estimated 30% of RVA positive samples worldwide. We have developed a VP7 and VP4 multiplexed one-step genotyping assays using updated primers generated from contemporary VP7 and VP4 sequences. To determine assay specificity and sensitivity, 17 reference virus strains, ...
DOI:
10.1016/j.jviromet.2015.07.012
PubMed:
26231786
PubMed Central:
PMC5818709

50. Pathogenicity of porcine G9P[23] and G9P[7] rotaviruses in piglets

Author:
Ha-Hyun Kim; Jun-Gyu Park; Jelle Matthijnssens; Hyun-Jeong Kim; Hyung-Jun Kwon; Kyu-Yeol Son; Eun-Hye Ryu; Deok-Song Kim; Woo Song Lee; Mun-Il Kang; Dong-Kun Yang; Ju-Hwan Lee; Su-Jin Park; Kyoung-Oh Cho
Source:
Veterinary microbiology 2013 v.166 no.1-2 pp. 123-137
ISSN:
0378-1135
Subject:
RNA; antigens; choroid plexus; disease reservoirs; feces; hepatocytes; humans; intestinal mucosa; lungs; lymph nodes; model validation; necrosis; pathogenesis; pathogenicity; pathogens; piglets; small intestine; viremia
Abstract:
... G9 group A rotaviruses (RVAs) are considered important pathogens in pigs and humans, and pigs are hypothesized to be a potential host reservoir for human. However, intestinal and extra-intestinal pathogenicity and viremia of porcine G9 RVAs has remained largely unreported. In this study, colostrum-deprived piglets were orally infected with a porcine G9P[23] or G9P[7] strain. Histopathologically, b ...
DOI:
10.1016/j.vetmic.2013.05.024
PubMed:
23827353