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You searched for: Subject term Rotavirus Remove constraint Subject term: Rotavirus Subject term color Remove constraint Subject term: color Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. Development and application of a loop-mediated isothermal amplification assay on rapid and sensitive detection of rotavirus in fecal samples and artificially seeded oysters

Author:
Xiaoxia Kou; Hongying Fan; Qingping Wu; Liang Xue; Jumei Zhang
Source:
Food control 2014 v.41 pp. 151-157
ISSN:
0956-7135
Subject:
RNA; Rotavirus; betaine; color; cross reaction; detection limit; feces; gel electrophoresis; genome; magnesium sulfate; monitoring; oysters; reverse transcription loop-mediated isothermal amplification; risk assessment; turbidity
Abstract:
... A reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of rotavirus. Four primers were designed to recognize six distinct regions based on a highly conserved sequence in the VP7 region of the rotavirus genome. The optimal conditions for the LAMP assay were determined to 62 °C for 60 min with 8.0 mM MgSO4, 0.2 M betaine, 0.8 mM dNTPs, 0.2 μM each ...
DOI:
10.1016/j.foodcont.2014.01.013

2. Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes

Author:
Paloma Juárez; Silvia Presa; Joaquín Espí; Benito Pineda; María T. Antón; Vicente Moreno; Javier Buesa; Antonio Granell; Diego Orzaez
Source:
Plant biotechnology journal 2012 v.10 no.3 pp. 341-352
ISSN:
1467-7644
Subject:
Antirrhinum majus; Rotavirus; anthocyanins; antibodies; color; fruits; humans; immunoglobulin A; neutralization tests; pathogens; tomatoes; transcription (genetics); transcription factors; transgenes
Abstract:
... Edible fruits are inexpensive biofactories for human health‐promoting molecules that can be ingested as crude extracts or partially purified formulations. We show here the production of a model human antibody for passive protection against the enteric pathogen rotavirus in transgenically labelled tomato fruits. Transgenic tomato plants expressing a recombinant human immunoglobulin A (hIgA_2A1) sel ...
DOI:
10.1111/j.1467-7652.2011.00666.x
PubMed:
22070155