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You searched for: Subject term Streptomyces Remove constraint Subject term: Streptomyces Subject term acetone Remove constraint Subject term: acetone Text availability Citation in PubAg Remove constraint Text availability: Citation in PubAg
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1. Antibacterial Cyclic Lipopeptide Enamidonins with an Enamide-Linked Acyl Chain from a Streptomyces Species

Author:
Sangkeun Son; Sung-Kyun Ko; Seung Min Kim; Eun Kim; Gil Soo Kim; Byeongsan Lee; In-Ja Ryoo; Won-Gon Kim; Jung-Sook Lee; Young-Soo Hong; Jae-Hyuk Jang; Jong Seog Ahn
Source:
Journal of natural products 2018 v.81 no.11 pp. 2462-2469
ISSN:
1520-6025
Subject:
Gram-positive bacteria; Staphylococcus aureus; Streptomyces; acetone; amino acids; antibacterial properties; antibiotic resistance; derivatization; linkage groups; lipopeptides; liquid chromatography; mass spectrometry; methicillin; nuclear magnetic resonance spectroscopy; Korean Peninsula
Abstract:
... Three cyclic lipopeptides, including one known (1) and two new (2 and 3) compounds, that possess the rare enamide linkage group were discovered from Streptomyces sp. KCB14A132, an actinobacterium isolated from a soil sample collected from Jeung Island, Korea. The NMR and MS-based characterization showed that they differed in the amino acid residues in the peptide backbone. Application of Marfey’s ...
DOI:
10.1021/acs.jnatprod.8b00497

2. Cloning, heterologous expression and characterization of a novel streptomyces trypsin in Bacillus subtilis SCK6

Author:
Zhikuan Wang; Xiaoguang Li; Jiewei Tian; Yiwen Chu; Yongqiang Tian
Source:
International journal of biological macromolecules 2020 v.147 pp. 890-897
ISSN:
0141-8130
Subject:
Bacillus subtilis; EDTA (chelating agent); Streptomyces; acetone; amidase; amino acids; calcium; chloroform; dimethyl sulfoxide; enzyme activity; enzyme inhibition; esterases; fermentation; glycerol; heterologous gene expression; hexane; iron; magnesium; methanol; pH; plasmids; potassium; temperature; trypsin
Abstract:
... A novel streptomyces trypsin GM2938 was selected as the object of study. The active GM2938 contains 223 amino acid residues. Constructing recombinant plasmid and transforming Bacillus subtilis SCK6, the heterogenous expression of GM2938 was achieved. Through optimization of fermentation conditions, the expression level of GM2938 reached 1622.2 U/mL (esterase activity) and 33.8 U/mL (amidase activi ...
DOI:
10.1016/j.ijbiomac.2019.09.248

3. Construction of a novel expression vector in Pseudonocardia autotrophica and its application to efficient biotransformation of compactin to pravastatin, a specific HMG-CoA reductase inhibitor

Author:
Yoshikazu Fujii; Koji Norihisa; Tadashi Fujii; Yasuhide Aritoku; Yusuke Kagawa; Khalid Ibrahim Sallam; Osamu Johdo; Akira Arisawa; Tomohiro Tamura
Source:
Biochemical and biophysical research communications 2011 v.404 no.1 pp. 511-516
ISSN:
0006-291X
Subject:
Pseudonocardia autotrophica; acetaldehyde dehydrogenase; acetone; anticholesteremic agents; biotransformation; cytochrome P-450; gene expression; genes; genetic transformation; hosts; hypercholesterolemia; plasmid vectors; plasmids; polyacrylamide gel electrophoresis; promoter regions; replication origin; spectral analysis
Abstract:
... The novel plasmid vector (pTAOR4-Rev) suitable for gene expression in actinomycete strains of Pseudonocardia autotrophica was constructed from 2 P. autotrophica genetic elements, the novel replication origin and the acetone-inducible promoter. The replication origin was isolated from the endogenous plasmid of strain DSM 43082 and the acetone-inducible promoter was determined by analysis of the ups ...
DOI:
10.1016/j.bbrc.2010.12.013
PubMed:
21144838

4. Eco-friendly approach using marine actinobacteria and its compounds to control ticks and mosquitoes

Author:
Mohankumar Thenmozhi; Jannu Vinay Gopal; Krishnan Kannabiran; Govindasamy Rajakumar; Kanayairam Velayutham; Abdul Abdul Rahuman
Source:
Parasitology research 2013 v.112 no.2 pp. 719-729
ISSN:
0932-0113
Subject:
ticks; acetates; high performance liquid chromatography; acaricidal properties; Streptomyces; Anopheles subpictus; mass spectrometry; malaria; chloroform; blood; acetone; Haemaphysalis; acids; mosquito control; synergism; databases; Culex quinquefasciatus; silica gel; larvae; methanol; Rhipicephalus; gas chromatography; thin layer chromatography
Abstract:
... Ticks and mosquitoes are ectoparasitic arthropods that can transmit a variety of diseases to humans and animals during blood feeding and causing serious infectious disorders. The purpose of the present study was to assess the acaricidal and insecticidal property of ethyl acetate extract and its compounds isolated from marine actinobacteria, Streptomyces VITSTK7 sp. against the larvae of cattle tic ...
DOI:
10.1007/s00436-012-3192-3
PubMed:
23180127

5. Organic solvent tolerance of an alkaline protease from salt-tolerant alkaliphilic Streptomyces clavuligerus strain Mit-1

Author:
Thumar, Jignasha T.; Singh, Satya P.
Source:
Journal of industrial microbiology & biotechnology 2009 v.36 no.2 pp. 211-218
ISSN:
1367-5435
Subject:
Streptomyces clavuligerus; acetone; benzene; butanol; carbon; catalytic activity; chloroform; ethanol; nucleotide sequences; pH; proteinases; ribosomal RNA; secretion; solvents; xylene; India
Abstract:
... A salt-tolerant alkaliphilic actinomycete, Mit-1 was isolated from Mithapur, coastal region of Gujarat, India. The strain was identified as Streptomyces clavuligerus and based on 16S rRNA gene sequence (EU146061) homology; it was related to Streptomyces sp. (AY641538.1). The organism could grow with up to 15% salt and pH 11, optimally at 5% and pH 9. It was able to tolerate and secrete alkaline pr ...
DOI:
10.1007/s10295-008-0487-6
PubMed:
18941814

6. Preparation and characterization of cross-linked enzyme aggregates (CLEAs) of recombinant poly-3-hydroxybutyrate depolymerase from Streptomyces exfoliatus

Author:
Daniel Hormigo; Javier García-Hidalgo; Carmen Acebal; Isabel de la Mata; Miguel Arroyo
Source:
Bioresource technology 2012 v.115 no. pp. 177-182
ISSN:
0960-8524
Subject:
Streptomyces exfoliatus; acetone; bovine serum albumin; catalytic activity; crosslinking; enzymes; hydrolysis; microscopy; pH; recycling; solvents; temperature
Abstract:
... Cross-linked enzyme aggregates of poly-3-hydroxybutyrate (PHB) depolymerase from Streptomyces exfoliatus (PhaZSₑₓ–CLEAs) have been prepared. Acetone was used as the precipitating agent, while addition of bovine serum albumin (BSA) facilitated CLEAs formation. Conditions for enzyme precipitation and cross-linking have been optimized, and confocal scanning microscopy showed a homogeneous enzyme dist ...
DOI:
10.1016/j.biortech.2011.09.035
PubMed:
21974880

7. Production, purification and characterization of cholesterol oxidase from a newly isolated Streptomyces sp.

Author:
Ram Niwas; Vineeta Singh; Rajbir Singh; Divya Tripathi; C. K. M. Tripathi
Source:
World journal of microbiology & biotechnology 2013 v.29 no.11 pp. 2077-2085
ISSN:
0959-3993
Subject:
Streptomyces; acetone; benzene; chemical oxygen demand; chloroform; cholesterol; dithiothreitol; enzyme activity; ethanol; fermentation; isopropyl alcohol; lactose; molecular weight; pH; sodium dodecyl sulfate; solvents; soybean meal; sucrose; temperature; yeast extract
Abstract:
... Cholesterol oxidase production (COD) by a new isolate characterized as Streptomyces sp. was studied in different production media and fermentation conditions. Individual supplementation of 1 % maltose, lactose, sucrose, peptone, soybean meal and yeast extract enhanced COD production by 80-110 % in comparison to the basal production medium (2.4 U/ml). Supplementation of 0.05 % cholesterol (inducer) ...
DOI:
10.1007/s11274-013-1371-8
PubMed:
23700127

8. Purification and Characterization of the Enzyme Cholesterol Oxidase from a New Isolate of Streptomyces sp.

Author:
Vandana Praveen; Akanksha Srivastava; C. K. M. Tripathi
Source:
Applied biochemistry and biotechnology 2011 v.165 no.5-6 pp. 1414-1426
ISSN:
0273-2289
Subject:
acetone; molecular weight; enzymes; ion exchange; sodium dodecyl sulfate; cholesterol; gel chromatography; oxidation; benzene; chloroform; ethanol; enzyme activity; Streptomyces parvus
Abstract:
... An extracellular cholesterol oxidase (cho) enzyme was isolated from the Streptomyces parvus, a new source and purified 18-fold by ion exchange and gel filtration chromatography. Specific activity of the purified enzyme was found to be 20 U/mg with a 55 kDa molecular mass. The enzyme was stable at pH 7.2 and 50 °C. The enzyme activity was inhibited in the presence of Pb2+, Ag2+, Hg2+, and Zn2+ and ...
DOI:
10.1007/s12010-011-9360-6
PubMed:
21909628

9. Purification and characterization of the protease enzymes of Streptomyces thermovulgaris grown in rapemeal-derived media

Author:
Yeoman, K.H.; Edwards, C.
Source:
Journal of applied microbiology 1997 v.82 no.2 pp. 149-156
ISSN:
1364-5072
Subject:
Streptomyces thermovulgaris; acetone; calcium; enzyme activity; ions; molecular weight; serine proteinases; thermal stability; ultrafiltration; yeast extract
Abstract:
... When grown in a particulate-free, protein-rich medium derived from rapemeal (termed medium B), Streptomyces thermovulgaris produced multiple protease enzymes. The main protease activity was attributed to two types of serine protease, denoted as SV1 and SV2. A metallo protease component (SV3) and an azocaseinase component (SV4) were also present. Protease SV1 had a molecular weight of 30 kDa and a ...
DOI:
10.1111/j.1365-2672.1997.tb03567.x
PubMed:
12452588

10. Screening and characterization of microbial inhibitors against eukaryotic protein phosphatases (PP1 and PP2A)

Author:
Ong, S.M.; Voo, L.Y.C.; Lai, N.S.; Stark, M.J.R.; Ho, C.C.
Source:
Journal of applied microbiology 2007 v.102 no.3 pp. 680-692
ISSN:
1364-5072
Subject:
Penicillium; Streptomyces; acetone; forest soils; hypersensitivity; mammals; reversed-phase high performance liquid chromatography; screening; yeasts; Borneo
Abstract:
... To identify novel microbial inhibitors of protein phosphatase 1 (PP1). 750 actinomycetes and 408 microfungi were isolated from Sabah forest soils and screened for production of potential PP1 inhibitors using an in vivo screening system, in which candidate inhibitors were identified through mimicking the properties of PP1-deficient yeast cells. Acetone extracts of two fungi, H9318 (Penicillium) and ...
DOI:
10.1111/j.1365-2672.2006.03135.x
PubMed:
17309617

11. Screening, isolation and evaluation of a nematicidal compound from actinomycetes against the pine wood nematode, Bursaphelenchus xylophilus

Author:
Min‐Jiao Liu; Byung‐Soon Hwang; Chun‐Zhi Jin; Wen‐Jun Li; Dong‐Jin Park; Sang‐Tae Seo; Chang‐Jin Kim
Source:
Pest management science 2019 v.75 no.6 pp. 1585-1593
ISSN:
1526-498X
Subject:
Bursaphelenchus xylophilus; Pinus densiflora; Streptomyces; acetone; active ingredients; coniferous forests; disease control; endoparasites; financial economics; forest ecosystems; forest soils; fractionation; greenhouse production; greenhouses; lethal concentration 50; metabolites; mycelium; nematicidal properties; nematicides; screening; soil sampling; trees; vascular wilt
Abstract:
... BACKGROUND: Bursaphelenchus xylophilus is a migratory endoparasitic nematode known to cause severe environmental damage and economic losses in pine forest ecosystems. This present study investigated the nematicidal metabolites of actinomycetes in vitro and evaluated the disease control efficacy of the active compound and metabolites under greenhouse and field conditions. RESULTS: Five thousand typ ...
DOI:
10.1002/ps.5272

12. Sugar Ester Synthesis by Thermostable Lipase from Streptomyces thermocarboxydus ME168

Author:
Aran H-Kittikun; Poonsuk Prasertsan; Wolfgang Zimmermann; Phisit Seesuriyachan; Thanongsak Chaiyaso
Source:
Applied biochemistry and biotechnology 2012 v.166 no.8 pp. 1969-1982
ISSN:
0273-2289
Subject:
dioxane; thermal stability; acetates; polyacrylamide gel electrophoresis; gel chromatography; acetone; hexanoic acid; sulfates; glucose; EDTA (chelating agent); Streptomyces thermocarboxydus; molecular weight; half life; butyrates; dithiothreitol; triacylglycerol lipase; temperature; ethanol; ion exchange chromatography; pH; solvents
Abstract:
... The extracellular lipase from Streptomyces thermocarboxydus ME168 was purified to 9.5-fold with 20% yield, following concentration by acetone precipitation, ion exchange chromatography (Resource Q) and gel filtration chromatography (Superdex 200), respectively. The purified enzyme had an apparent molecular mass of 21 kDa by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The ...
DOI:
10.1007/s12010-012-9624-9
PubMed:
22434352