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... The primary objective of this study was to determine how various crop species differ in their cation-anion balance and thus in their effects on soil reaction and on the acidifying effects of N fertilizers on the base status of soils. Samples of 27 species of cereal, grass, legume, and miscellaneous field and vegetable crops were collected from a wide range of environmental conditions in the field. ...
... The auxin-like properties of 4-amino-3,5,6-trichloropicolinic acid (picloram) were compared with those of other auxins in tissue cultures of wheat (Triticum aestivum), soybeans (Glycine max), tobacco (Nicotiana tabacum), and jackbean (Canavalia ensiformis) explants. Certain problems associated with the use of 2,4-dichlorophenoxyacetic acid (2,4-D) in tissue culture were not encountered when piclor ...
... In eubacterial and eukaryotic tRNAs specific for Asn, Asp, His and Tyr the modified deazaguanosine-derivative queuosine occurs in position 34, the first position of the anticodon. Analysis of unfractionated tRNAs from wheat and from tobacco leaves shows that these tRNAs contain high amounts of guanosine (G) in place of queuosine (Q). This was measured by the exchange of G34 for [3H]guanine catalys ...
... Purified spinach (Spinacea oleraced L.) and barley (Hordeum vulgare L.) ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase supported 50 to 100% activation of substrate-bound Rubisco from spinach, barley, wheat (Triticum aestivum L.), soybean (Glycine max L.), pea (Pisum sativum L.), Arabidopsis thaliana, maize (Zea mays L.), and Chlamydomonas reinhardtii but supported only 10 to 35 ...
ribulose-bisphosphate carboxylase; carbon dioxide; radionuclides; isotope labeling; Hordeum vulgare; chemical reactions; gas production (biological); oxygenases; photosynthates; radiometry; carbon; Triticum aestivum; Nicotiana tabacum; photosynthesis; oxygen
Abstract:
... A radiogasometric method for the determination of the rates of intracellular decarboxylation of primary and end products of steady-state photosynthesis in the light has been worked out. On the basis of oxygen dependence of the CO2 evolution, two types of decarboxylation, oxygenase (photorespiration) and oxidase (dark respiration), have been distinguished. Oxygenase decarboxylation is linearly depe ...
... Two different methods were used to investigate the abundance of cytosine methylation at the outer (5') position in 5'-CCG-3' trinucleotides in angiosperm genomes. MspI is unable to cut its target site if the outer cytosine is methylated (5'-mCCGG-3'). Using MspI restriction analysis, it was shown that 5'-mCCG-3' is present in all angiosperm genomes examined, and that the amount of cytosine methyla ...
... Analyses of a series of allelic chlorina mutants of wheat (Triticum aestivum L.), which have partial blocks in chlorophyll (Chl) synthesis and, therefore, a limited Chl supply, reinforce the principle that Chl is required for the stable accumulation of Chl-binding proteins and that only reaction centers accumulate when the supply of Chl is severely limited. Depending on the rate of Chl accumulatio ...
... We investigated the role of actin microfilaments in nonhost resistance of higher plants. Here we present several lines of evidence to indicate that microfilaments are indeed involved in blocking fungal penetration of nonhost plants. Erysiphe pisi, a pathogen of pea, normally fails to penetrate into nonhost plants such as barley, wheat, cucumber and tobacco. When tissues of these nonhost plants wer ...
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...
... Hexaploid wheat (Triticum aestivum L.) has very low constitutive glutathione S-transferase (GST) activity when assayed with the chloroacetamide herbicide dimethenamid as a substrate, which may account for its low tolerance to dimethenamid in the field. Treatment of seeds with the herbicide safener fluxofenim increased the total GST activity extracted from T. aestivum shoots 9-fold when assayed wit ...
... The high cash value of burley tobacco (Nicotiana tabacum L.) may lead to ineffective excess fertilization Of the crop. The effects of N management on fertilizer N use efficiency by hurley tobacco and recovery of residual fertilizer N by a winter cover crop were determined. Tobacco was grown in 1993 at two locations with 0 to 336 kg N ha⁻¹ broadcast applied before transplanting or 168 kg N ha⁻¹ sid ...
... Wild-type and mutated forms of the wheat (Triticum aestivum L.) storage protein gamma-gliadin were expressed in transgenic tobacco (Nicotiana tabacum L. cv. NVS) under the control of the 35S cauliflower mosaic virus (CaMV) promoter in order to determine what, if any, endogenous targeting signals are present in the wild-type gamma-gliadin protein. The mutant forms of the protein were modified by th ...
... A cDNA library made from mRNA of Al-treated roots of an Al-sensitive wheat (Triticum aestivum cv Victory) cultivar was screened with a degenerate oligonucleotide probe derived from the partial amino acid sequence of the Al-induced protein TAl-18. Of seven clones that initially hybridized with the probe, one encoded a novel 1,3-beta-glucanase having a calculated molecular weight of 46.3 and an isoe ...
... Dichloroacetamide safeners protect maize (Zea mays L.) against injury from chloroacetanilide and thiocarbamate herbicides. Etiolated maize seedlings have a high-affinity cytosolic-binding site for the safener [3H](R,S)-3-dichloroacetyl-2,2,5-trimethyl-1, 3-oxazolidine ([3H]Saf), and this safener-binding activity (SafBA) is competitively inhibited by the herbicides. The safener-binding protein (Saf ...
... A comparison of the wild-type firefly luciferase reporter gene to a codon-modified gene, available from Promega, demonstrates that in tobacco cell cultures, an increase in G+C content of 1.8%, as a consequence of 36 A/T leads to G/C synonymous codon alterations and removal of the lysosomal targeting sequence, has no significant effect on expression. In maize Black Mexican Sweet cells and wheat scu ...
... Transcription of Cab genes has been previously shown to be regulated by a circadian oscillator coupled to the red light-absorbing plant photoreceptor phytochrome in various plant species. In addition, it has recently been suggested that rhythmic expression of the Cab genes could also be affected by a phytochrome-independent circadian oscillator in a developmentally regulated fashion. This study ha ...
... Immunofluorescence staining with antisera raised against p35, a basic nuclear protein that accumulates in the pollen nuclei of Lilium longiflorum, specifically stained the nucleoli in interphase nuclei of somatic tissues, including root and leaf, and in pachytene nuclei during meiotic division, whereas antisera raised against histone H1 uniformly stained the entire chromatin domain with the except ...
... Although cytokinins (CKs) affect a number of processes connected with chloroplasts, it has never been rigorously proven that chloroplasts contain CKs. We isolated intact chloroplasts from tobacco (Nicotiana tabacum L. cvSR1) and wheat (Triticum aestivum L. cv Ritmo) leaves and determined their CKs by liquid chromatography/tandem mass spectroscopy. Chloroplasts from both species contained a whole s ...