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You searched for: Subject term Triticum aestivum Remove constraint Subject term: Triticum aestivum Subject term beta-glucuronidase Remove constraint Subject term: beta-glucuronidase
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1. pEmu: an improved promoter for gene expression in cereal cells

Author:
Last, D.I.; Brettell, R.I.S.; Chamberlain, D.A.; Chaudhury, A.M.; Larkin, P.J.; Marsh, E.L.; Peacock, W.J.; Dennis, E.S.
Source:
Theoretical and applied genetics 1991 v.81 no.5 pp. 581-588
ISSN:
0040-5752
Subject:
Triticum aestivum; Zea mays; Oryza sativa; Triticum monococcum; Lolium multiflorum; Nicotiana plumbaginifolia; genetic transformation; gene expression; protoplasts; direct DNA uptake; electroporation; recombinant DNA; reporter genes; beta-glucuronidase; promoter regions
Abstract:
... A recombinant promoter, pEmu, has been constructed to give a high level of gene expression in monocots. It is based on a truncated maize Adh1 promoter, with multiple copies of the Anaerobic Responsive Element from the maize Adh1 gene and ocs-elements from the octopine synthase gene of Agrobacterium tumefaciens. The pEmu promoter was one of 12 different promoter constructs that were linked to the b ...
DOI:
10.1007/BF00226722
PubMed:
24221371

2. Factors affecting transient gene expression in protoplasts isolated from very slowly growing embryogenic callus cultures of wheat (Triticum aestivum L.)

Author:
Zaghmout, O.M.F.; Trolinder, N.L.
Source:
Theoretical and applied genetics 1993 v.86 no.6 pp. 721-730
ISSN:
0040-5752
Subject:
alcohol dehydrogenase; protoplasts; genetic transformation; Tobacco mosaic virus; recombinant DNA; transgenic plants; enzyme activity; plasmid vectors; gene expression; histochemistry; Triticum aestivum; reporter genes; Cauliflower mosaic virus; Vitis vinifera; embryogenesis; promoter regions; beta-glucuronidase; callus; electroporation
Abstract:
... Protoplasts isolated from embryogenic ('Mustang' and 'Chinese Spring') and non-embryogenic ('Mit') calli of wheat (Triticum aestivum L.) genotypes transiently expressed beta-glucuronidase (GUS) activity when electroporated with a plasmid containing the GUS gene and driven by an enhanced 35S promoter and a TMV leader sequence. Conditions for the maximum expression of GUS activity were: electroporat ...
DOI:
10.1007/BF00222662
PubMed:
24193782

3. Spatial-temporal colonization patterns of Azospirillum brasilense on the wheat root surface and expression of the bacterial nifH gene during association

Author:
Broek, A.V.; Michiels, J.; Gool, A. van.; Vanderleyden, J.
Source:
Molecular plant-microbe interactions 1993 v.6 no.5 pp. 592-600
ISSN:
0894-0282
Subject:
temporal variation; promoter regions; nitrogen fixation; recombinant DNA; spatial distribution; Azospirillum brasilense; histochemistry; Triticum aestivum; adhesion; acetylene; reporter genes; nitrogenase; roots; beta-glucuronidase
DOI:
10.1094/MPMI-6-592

4. Fertile transgenic wheat from microprojectile bombardment of scutellar tissue

Author:
Becker, D.; Brettschneider, R.; Lorz, H.
Source:
plant journal 1994 v.5 no.2 pp. 299-307
ISSN:
0960-7412
Subject:
genetic transformation; transgenic plants; embryo (plant); Oryza sativa; plasmids; gene expression; Triticum aestivum; genes; artificial regeneration; actin; enzyme activity; beta-glucuronidase; genetic markers; tissue culture
DOI:
10.1046/j.1365-313X.1994.05020299.x
PubMed:
8148881

5. Transformation of protoplasts and intact cells from slowly growing embryogenic callus of wheat (Triticum aestivum L.)

Author:
Zaghmout, O.M.F.
Source:
Theoretical and applied genetics 1994 v.89 no.5 pp. 577-582
ISSN:
0040-5752
Subject:
protoplasts; genetic transformation; Agrobacterium radiobacter; direct DNA uptake; gene expression; polyethylene glycol; Triticum aestivum; reporter genes; embryogenesis; beta-glucuronidase; callus; electroporation
Abstract:
... A procedure for culturing protoplasts from slowly growing embryogenic calli of wheat was developed. The procedure was dependent on the ability to isolate large numbers of culturable protoplasts from slowly growing embryogenic callus. Approximately 68% of the isolated protoplasts divided, and 22% formed colonies; of the latter, 67% continued to proliferate. Plating efficiency was reduced when proto ...
DOI:
10.1007/BF00222451
PubMed:
24177933

6. Adenine methylation at dam sites increases transient gene expression in plant cells

Author:
Graham, M.W.; Larkin, P.J.
Source:
Transgenic research 1995 v.4 no.5 pp. 324-331
ISSN:
0962-8819
Subject:
Triticum aestivum; Nicotiana plumbaginifolia; Trifolium subterraneum; DNA methylation; adenine; Escherichia coli; gene transfer; genetic transformation; gene expression; reporter genes; beta-glucuronidase; recombinant DNA; protoplasts; electroporation; promoter regions
DOI:
10.1007/BF01972529
PubMed:
8589735

7. Molecular characterization of the fate of transgenes in transformed wheat (Triticum aestivum L.)

Author:
Srivastava, V.; Vasil, V.; Vasil, I.K.
Source:
Theoretical and applied genetics 1996 v.92 no.8 pp. 1031-1037
ISSN:
0040-5752
Subject:
Southern blotting; gene transfer; DNA methylation; phosphinothricin acetyltransferase; transgenic plants; gene expression; Triticum aestivum; reporter genes; inheritance (genetics); genetic variation; enzyme activity; histochemistry; beta-glucuronidase
Abstract:
... Molecular analysis of the transgenes bar and gus was carried out over successive generations in six independent transgenic lines of wheat, until the plants attained homozygosity. Data on expression and integration of the transgenes is presented. Five of the lines were found to be stably transformed, duly transferring the transgenes to the next generation. The copy number of the transgenes varied f ...
DOI:
10.1007/BF00224045
PubMed:
24166632

8. Cooperative of two distinct cis-acting elements is necessary for the S phase-specific activation of the wheat histone H3 promoter

Author:
Ohtsubo, N.; Nakayama, T.; Kaya, H.; Terada, R.; Shimamoto, K.; Meshi, T.; Iwabuchi, M.
Source:
plant journal 1997 v.11 no.6 pp. 1219-1225
ISSN:
0960-7412
Subject:
promoter regions; messenger RNA; histones; Oryza sativa; transgenic plants; plasmid vectors; mutation; gene expression; Triticum aestivum; interphase; beta-glucuronidase
Abstract:
... We have previously shown that the proximal promoter region (-185 to +57) of the wheat histone H3 gene (TH012) is sufficient for regulating S phase-expression of a reporter GUS gene. To define the cis-acting element(s) responsible for S phase-specific expression, GUS fusion genes under the control of wild-type or variously mutated H3 promoters were stably introduced into cultured rice Oc cells and ...
DOI:
10.1046/j.1365-313X.1997.11061219.x

9. Genetic transformation of wheat mediated by Agrobacterium tumefaciens

Author:
Cheng, M.; Fry, J.E.; Pang, S.Z.; Zhou, H.P.; Hironaka, C.M.; Duncan, D.R.; Conner, T.W.; Wan, Y.C.
Source:
Plant physiology 1997 v.115 no.3 pp. 971-980
ISSN:
0032-0889
Subject:
developmental stages; neomycin; inheritance (genetics); genetic markers; callus; Agrobacterium radiobacter; explants; gene expression; Triticum aestivum; plant development; phosphotransferases (phosphomutases); enzyme activity; histochemistry; genetic transformation; gene transfer; transgenic plants; embryo (plant); rapid methods; plant morphology; genetic vectors; beta-glucuronidase
Abstract:
... A rapid Agrobacterium tumefaciens-mediated transformation system for wheat was developed using freshly isolated immature embryos, precultured immature embryos, and embryogenic calli as explants. The explants were inoculated with a disarmed A. tumefaciens strain C58 (ABI) harboring the binary vector pMON18365 containing the beta-glucuronidase gene with an intron, and a selectable marker, the neomyc ...

10. Importance of the chiral centers of jasmonic acid in the responses of plants. Activities and antagonism between natural and synthetic analogs

Author:
Holbrook, L.; Tung, P.; Ward, K.; Reid, D.M.; Abrams, S.; Lamb, N.; Quail, J.W.; Moloney, M.M.
Source:
Plant physiology 1997 v.114 no.2 pp. 419-428
ISSN:
0032-0889
Subject:
Helianthus annuus; Brassica napus; measurement; chemical degradation; dose response; jasmonic acid; gene expression; Triticum aestivum; Nicotiana tabacum; esters; stereochemistry; enzyme activity; chemical structure; seed germination; transgenic plants; cotyledons; proteinase inhibitors; reporter genes; chemical constituents of plants; chlorophyll; beta-glucuronidase
Abstract:
... The importance of the two chiral centers at C-3 and C-7 in the molecular structure of jasmonic acid in plant responses was investigated. We separated methyl jasmonate (MeJA) into (3R)- and (3S)-isomers with a fixed stereochemistry at C-3, but epimerization at C-7 is possible. The four isomers of the nonepimerizable analog 7-methyl MeJA were synthesized. These six esters and their corresponding aci ...

11. A transient assay for evaluating promoters in wheat endosperm tissue

Author:
Clarke, B.C.; Appels, R.
Source:
Genome = 1998 v.41 no.6 pp. 865-871
ISSN:
0831-2796
Subject:
genetic transformation; biolistics; chromosomes; assays; prolamins; gene expression; endosperm; Triticum aestivum; reporter genes; enzyme activity; promoter regions; beta-glucuronidase
Abstract:
... A transient assay was developed for the evaluation of promoter sequences in wheat endosperm tissue. A deletion series from an omega-secalin gene promoter, located on chromosome 1RS.1DL of specific wheat lines, were translationally fused to a uidA reporter gene. These promoters were evaluated for expression in wheat endosperm tissue after integration of the DNA into the cell using microprojectile b ...
DOI:
10.1139/gen-41-6-865
PubMed:
9924795

12. Endophytic establishment of diazotrophic bacteria in auxin-induced tumors of cereal crops

Author:
Christiansen-Weniger, C.
Source:
Critical reviews in plant sciences 1998 v.17 no.1 pp. 55-76
ISSN:
0735-2689
Subject:
Triticum aestivum; Zea mays; Oryza sativa; root nodules; auxins; hypocotyls; neoplasms; root meristems; Azospirillum; Rhizobium; nitrogen-fixing bacteria; cortex; infection; cytoplasm; endophytes; nitrogenase; enzyme activity; carbon; histology; beta-glucuronidase; genetic markers; nitrogen fixation; photosynthesis; translocation (plant physiology); Azorhizobium caulinodans; ammonium compounds
DOI:
10.1080/07352689891304168

13. A transient assay system for the functional assessment of defense-related genes in wheat

Author:
Schweizer, P.; Pokorny, J.; Abderhalden, O.; Dudler, R.
Source:
Molecular plant-microbe interactions 1999 v.12 no.8 pp. 647-654
ISSN:
0894-0282
Subject:
biolistics; phenotype; molecular weight; assays; transgenic plants; plasmid vectors; plant proteins; Blumeria graminis f. sp. tritici; gene expression; fungi; Triticum aestivum; reporter genes; cell wall components; enzyme activity; histochemistry; beta-glucuronidase; leaves; defense mechanisms
Abstract:
... The generation and characterization of transgenic wheat plants is a tedious and time-consuming process that limits the number of putatively important transgenes that can be tested. We therefore established a transient assay system based on wheat leaves to study the effect of transiently expressed genes on the interaction with the wheat powdery mildew fungus Erysiphe (syn. Blumeria) graminis f. sp. ...
DOI:
10.1094/MPMI.1999.12.8.647

14. Analysis of particle bombardment parameters to optimise DNA delivery into wheat tissues

Author:
Rasco-Gaunt, S.; Riley, A.; Barcelo, P.; Lazzeri, P.A.
Source:
Plant cell reports 1999 v.19 no.2 pp. 118-127
ISSN:
0721-7714
Subject:
calcium chloride; genetic transformation; inflorescences; inorganic ions; biolistics; transgenic plants; calcium; plasmid vectors; spermidine; particle size; gene expression; Triticum aestivum; reporter genes; beta-glucuronidase; tissue culture
Abstract:
... The objective of this study was to identify the major parameters controlling DNA delivery by particle bombardment to wheat (Triticum aestivum L.) scutellum and inflorescence tissue. The main factors studied were the DNA/gold precipitation process, bombardment parameters and tissue culture variables. Efficiency of DNA (uidA gene) delivery was assessed by scoring transient GUS expression in bombarde ...
DOI:
10.1007/s002990050721

15. Transient gene expression and influence of promoters on foreign gene expression in Arabidopsis thaliana

Author:
Gandhi, R.; Maheshwari, S.C.; Khurana, P.
Source:
In vitro cellular & developmental biology 1999 v.35 no.3 pp. 232-237
ISSN:
1054-5476
Subject:
Arabidopsis thaliana; gene expression; transgenic plants; mesophyll; protoplasts; polyethylene glycol; beta-glucuronidase; plasmid vectors; DNA; magnesium; cations; enzyme activity; reporter genes; Oryza sativa; Triticum aestivum; Brassica; promoter regions; inorganic ions; calcium
DOI:
10.1007/s11627-999-0084-z

16. Variations in the maize Ac transposase transcript level and the Ds excision frequency in transgenic wheat callus lines

Author:
Takumi, S.; Murai, K.; Mori, N.; Nakamura, C.
Source:
Genome = 1999 v.42 no.6 pp. 1234-1241
ISSN:
0831-2796
Subject:
Triticum aestivum; Zea mays; messenger RNA; transgenic plants; callus; transposons; beta-glucuronidase; reporter genes; biolistics; gene expression; nucleotidyltransferases; plasmids; promoter regions
Abstract:
... To investigate the excision of a maize transposable element in wheat cells, plasmid DNAs containing a Dissociation (Ds) element located between a rice actin 1 gene promoter and a beta-glucuronidase (GUS) gene (gus) were introduced into wheat callus lines by microprojectile bombardment, and transient GUS expression was assayed. The gus-expressing cells after Ds excision were detected only when the ...
DOI:
10.1139/g99-077

17. A matrix attachment region is located upstream from the high-molecular-weight glutenin gene Bx7 in wheat (Triticum aestivum L.)

Author:
Rampitsch, C.; Jordan, M.C.; Cloutier, S.
Source:
Genome 2000 v.43 no.3 pp. 483-486
ISSN:
0831-2796
Subject:
glutenins; alleles; loci; biolistics; transgenic plants; polymerase chain reaction; codons; gene expression; chromosome mapping; Triticum aestivum; reporter genes; DNA-binding domains; molecular weight; promoter regions; beta-glucuronidase; leaves
Abstract:
... A 2.2-kb nucleotide sequence rich in AT, located upstream from Bx7 allele of the high-molecular-weight glutenin Glu-B1 locus in wheat (Triticum aestivum cv. Glenlea) was cloned following amplification by PCR. The 5' region of this sequence contains motifs typically found in matrix attachment regions (MARs) in other plants. We have shown that part of the 2.2-kb DNA binds to wheat nuclear matrix (NM ...
DOI:
10.1139/g00-011
PubMed:
10902712

18. A plant virus vector for systemic expression of foreign genes in cereals

Author:
Choi, I.R.; Stenger, D.C.; Morris, T.J.; French, R.
Source:
plant journal 2000 v.23 no.4 pp. 547-555
ISSN:
0960-7412
Subject:
Wheat streak mosaic virus; seedlings; roots; interspecific variation; Hordeum vulgare; open reading frames; gene expression; Triticum aestivum; virulence; leaves; infection; coat proteins; Zea mays; polymerase chain reaction; histochemistry; genetic transformation; Avena sativa; reporter genes; genetic vectors; beta-glucuronidase
DOI:
10.1046/j.1365-313x.2000.00820.x
PubMed:
10972881

19. Elucidation of Septoria tritici x wheat interactions using GUS-expressing isolates

Author:
Pnini-Cohen, S.; Zilberstein, A.; Schuster, S.; Sharon, A.; Eyal, Z.
Source:
Phytopathology 2000 v.90 no.3 pp. 297-304
ISSN:
0031-949X
Subject:
Triticum aestivum; Mycosphaerella graminicola; plant pathogenic fungi; host-parasite relationships; virulence; beta-glucuronidase; enzyme activity; diagnostic techniques; genetic transformation; gene transfer; genetic markers; reporter genes; gene expression
Abstract:
... Isolate ISR398 of Septoria tritici (which produces none to few pycnidia on the wheat cv. Seri 82 and high coverage on cv. Shafir) and isolate ISR8036 (which is virulent on both cultivars) were genetically cotransformed using the selectable marker gene hph, which confers resistance to hygromycin B (hygB), and the reporter gene uidA, encoding beta-glucuronidase (GUS). Most of the genetically transfo ...
DOI:
10.1094/PHYTO.2000.90.3.297
PubMed:
18944623

20. Evaluation of Dasypyrum villosum populations for resistance to cereal eyespot and stripe rust pathogens

Author:
Yildirim, A.; Jones, S.S.; Murray, T.D.; Line, R.F.
Source:
Plant disease 2000 v.84 no.1 pp. 40-44
ISSN:
0191-2917
Subject:
germplasm; rust diseases; population; genetic resistance; strain differences; Oculimacula yallundae; chromosome mapping; plant genetic resources; disease resistance; Dasypyrum villosum; Triticum aestivum; provenance; chromosome translocation; Puccinia striiformis; beta-glucuronidase; Italy; Greece; Yugoslavia; Turkey (country)
Abstract:
... Resistance to Pseudocercosporella herpotrichoides (cause of eyespot) and Puccinia striiformis (cause of stripe rust) was evaluated in a germ plasm collection of Dasypyrum villosum (syn. Haynaldia villosa) and a set of disomic addition lines, a substitution, and a translocation line of D. villosum chromosomes in a wheat background. Three races of P. striiformis and a beta-glucuronidase-transformed ...
Handle:
10113/48436
DOI:
10.1094/PDIS.2000.84.1.40