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- Ventura-Juárez, Javier, et al. Show all 9 Authors
- Biotechnology letters 2017 v.39 no.8 pp. 1149-1157
- hamsters; transgenes; Komagataella pastoris; antigens; glycosylation; antibodies; polyacrylamide gel electrophoresis; polymerase chain reaction; recombinant fusion proteins; Entamoeba histolytica; staining; Western blotting; clones; plasmids; synthetic genes; vaccines; immunization; Pseudomonas aeruginosa; exotoxins
- ... OBJECTIVE: To generate an immunogenic chimeric protein containing the Entamoeba histolytica LC3 fragment fused to the retrograde delivery domains of exotoxin A of Pseudomonas aeruginosa and KDEL3 for use as an effective vaccine. RESULTS: A codon-optimized synthetic gene encoding the PEΔIII-LC3-KDEL3 fusion construct was designed for expression in Pichia pastoris. This transgene was subcloned into ...