Subject term: Western blotting and adults

1. Expression of Leˣ antigen in Schistosoma japonicum and S.haematobium and immune responses to Leˣ in infected animals: Lack of Leˣ expression in other trematodes and nematodes

Author:: A. Kwame Nyame; Russell Debose-Boyd; Troy D. Long; Victor C.W. Tsang; Richard D. Cummings
Source:: Glycobiology 1998 v.8 no.6 pp. 615-624
ISSN:: 1460-2423
Subject:: Caenorhabditis elegans; Dirofilaria immitis; Fasciola hepatica; Haemonchus contortus; Lotus tetragonolobus; Schistosoma japonicum; Schistosoma mansoni; Western blotting; Wisteria floribunda; adults; agglutinins; antigens; autoimmunity; detergents; dogs; enzyme-linked immunosorbent assay; free-living nematodes; glycoproteins; heartworms; humans; immune response; intestines; molecular weight; monoclonal antibodies; polysaccharides; rodents; ruminants
Abstract:: ... Adults of the human parasitic trematode Schistosoma mansoni, which causes hepatosplenic/intestinal complications in humans, synthesize glycoconjugates containing the Lewis x (Leˣ) Galβ1→4(Fucα1→3)GlcNAcβ1→R, but not sialyl Lewis x (sLeˣ), antigen. We now report on our analyses of Leˣ and sLeˣ expression in S.haematobium and S.japonicum, which are two other major species of human schistosomes that ...
DOI:: 10.1093/glycob/8.6.615
: https://doi.org/10.1093/glycob/8.6.615

2. Expression of the Lewis group carbohydrate antigens during Xenopus development

Author:: Chikako Yoshida-Noro; Janet Heasman; Kim Goldstone; Lucinda Vickers; Chris Wylie
Source:: Glycobiology 1999 v.9 no.12 pp. 1323-1330
ISSN:: 1460-2423
Subject:: Western blotting; Xenopus laevis; adults; animal ovaries; antibodies; antigens; brain; cell adhesion; cement glands; digestive system; embryo (animal); endothelial cells; glycoproteins; head; kidneys; leukocytes; ligands; lungs; mice; muscles; neurites; retina; spleen; stem cells; tadpoles; testes; tissues; toads
Abstract:: ... We have examined the pattern of expression of the Lewis group carbohydrate antigens during the development of African toad Xenopus laevis. One of these antigens, Lewis x (Leˣ, also known as SSEA-1), was previously shown to be involved in cell-cell adhesion in early mouse embryos and teratocarcinoma stem cells. Recently another member of these antigens, sialyl-Leˣ, was found to be one of the major ...
DOI:: 10.1093/glycob/9.12.1323
: https://doi.org/10.1093/glycob/9.12.1323

3. Ontogeny and subcellular localization of rat liver mitochondrial branched chain amino‐acid aminotransferase

Author:: Nimbe Torres; Carolina Vargas; Rogelio Hernández‐Pando; Héctor Orozco; Susan M. Hutson; Armando R. Tovar
Source:: European journal of biochemistry 2001 v.268 no.23 pp. 6132-6139
ISSN:: 0014-2956
Subject:: Western blotting; adults; antibodies; blood cells; chemical structure; gene expression; heart; hepatocytes; liver; messenger RNA; mitochondria; ontogeny; pregnancy; proteins; rats
Abstract:: ... Branched chain amino‐acid aminotransferase (BCAT) activity is present in fetal liver but the developmental pattern of mitochondrial BCAT (BCATm) expression in rat liver has not been studied. The aim of this study was to determine the activity, protein and mRNA concentration of BCATm in fetal and postnatal rat liver, and to localize this enzyme at the cellular and subcellular levels at both develop ...
DOI:: 10.1046/j.0014-2956.2001.02563.x
: https://doi.org/10.1046/j.0014-2956.2001.02563.x

4. UV‐A‐related alterations of young and adult lens water‐insoluble α‐crystallin, plasma membranous and cytoskeletal proteins

Author:: O. Weinreb; A. Dovrat; I. Dunia; E. L. Benedetti; H. Bloemendal
Source:: European journal of biochemistry 2001 v.268 no.3 pp. 536-543
ISSN:: 0014-2956
Subject:: Northern blotting; Western blotting; actin; adults; cattle; cytoskeleton; fiber cells; heat shock proteins; irradiation; membrane proteins; messenger RNA; organ culture; polymerase chain reaction; two-dimensional gel electrophoresis; ultraviolet radiation; vimentin
Abstract:: ... The damaging effects of UV‐A irradiation on lens water‐insoluble α‐crystallin, plasma membranous and cytoskeletal proteins derived from bovine lenses were studied. Young and adult bovine lenses were kept viable for 2 months in organ culture. After 24 h of incubation they were irradiated, and analyses of the proteins by one‐dimensional and two‐dimensional gel electrophoresis followed by Western blo ...
DOI:: 10.1046/j.1432-1327.2001.01885.x
: https://doi.org/10.1046/j.1432-1327.2001.01885.x

5. Cellular Distribution of Anionic Antimicrobial Peptide in Normal Lung and during Acute Pulmonary Inflammation

Author:: A. J. Fales-Williams; K. A. Brogden; E. Huffman; J. M. Gallup; M. R. Ackermann
Source:: Veterinary pathology v.39 no.6 pp. 706-711
ISSN:: 1544-2217
Subject:: Mannheimia; Pasteurella; Pseudomonas aeruginosa; Western blotting; adults; animal pathology; antibodies; antimicrobial peptides; bacteria; bronchioles; calves; cows; humans; immunohistochemistry; inflammation; inoculum; lipopolysaccharides; respiratory tract diseases; sheep
Abstract:: ... Anionic peptides (APs) are small antimicrobial peptides present in human and ovine lung. In this study APs were also detected in bovine lung, and production of APs in lungs with acute inflammation induced by various stimuli was determined. The distribution and intensity of APs were determined by immunohistochemistry in lungs of 1) neonatal calves (1-3 days of age) inoculated with Mannheimia (Paste ...
DOI:: 10.1354/vp.39-6-706
: https://doi.org/10.1354/vp.39-6-706

6. Acute effects of ethanol on feeding behavior and leptin-induced STAT3 phosphorylation in rat hypothalamus

Author:: Fujita, N.; Sakamaki, H.; Uotani, S.; Takahashi, R.; Kuwahara, H.; Kita, A.; Oshima, K.; Yamasaki, H.; Yamaguchi, Y.; Eguchi, K.
Source:: International journal of obesity 2003 v.27 no.1 pp. 55-59
ISSN:: 0307-0565
Subject:: Western blotting; acute effects; adults; appetite; body weight; drinking; energy balance; ethanol; feeding behavior; food intake; hypothalamus; intraperitoneal injection; leptin; phosphorylation; rats; satiety; signal transduction; transcription factors; tyrosine
Abstract:: ... OBJECTIVE: Drinking ethanol stimulates the appetite, producing a positive energy balance. The mechanism by which ethanol regulates the appetite in the central nervous system, however, has not been fully understood. The aim of this study is to investigate the interaction of ethanol with the satiety effect of leptin, a hormone which suppresses the appetite in the hypothalamic region. DESIGN: Leptin ...
DOI:: 10.1038/sj.ijo.0802194
PubMed:: 12532154
: https://doi.org/10.1038/sj.ijo.0802194

7. Humoral and cellular response in lambs vaccinated against Haemonchus contortus with p26/23

Author:: Domı́nguez-Toraño, I.A.; Fernández-Pérez, F.J.; Gómez-Muñoz, M.T.; Alunda, J.M.; Cuquerella, M.
Source:: Small ruminant research 2003 v.50 no.1-2 pp. 29-37
ISSN:: 0921-4488
Subject:: Haemonchus contortus; Western blotting; adults; antibodies; antigens; correlated responses; lambs; lymph nodes; parasites; peptides
Abstract:: ... Antigen recognition patterns (Western blotting) and lymphoproliferative response (local and peripheral) have been determined in lambs immunised and partially protected against haemonchosis with p26/23, a purified somatic fraction of Haemonchus contortus. Immunoprotective response induced by p26/23 was accompanied by both specific lymphoproliferative response and serum anti-Haemonchus antibodies. W ...
DOI:: 10.1016/S0921-4488(03)00105-6
: https://doi.org/10.1016/S0921-4488(03)00105-6

8. Monoclonal anti-equine IgE antibodies with specificity for different epitopes on the immunoglobulin heavy chain of native IgE

Author:: Wagner, Bettina; Radbruch, Andreas; Rohwer, Jens; Leibold, Wolfgang
Source:: Veterinary immunology and immunopathology 2003 v.92 no.1-2 pp. 45-60
ISSN:: 0165-2427
Subject:: Icelandic (horse breed); Western blotting; adults; basophils; blood serum; eczema; enzyme-linked immunosorbent assay; eosinophils; epitopes; fluorescent antibody technique; foals; hypersensitivity; immunoglobulin A; immunoglobulin E; immunoglobulin G; immunoglobulin M; immunoglobulin heavy chains; lymphocytes; mice; monoclonal antibodies; monocytes; sorting
Abstract:: ... In this study we describe the generation of monoclonal antibodies (mAbs), which recognize different epitopes of the equine IgE constant heavy chain. Equi-murine recombinant IgE (rIgE), composed of the murine V(H)186.2 heavy chain variable region, linked to the equine IgE constant heavy chain and expressed together with the murine λ1 chain in J558L cells was used to immunize BALB/C mice. A total of ...
DOI:: 10.1016/S0165-2427(03)00007-2
: https://doi.org/10.1016/S0165-2427(03)00007-2

9. Phase- and Age-Related Differences in Protein Tyrosine Phosphorylation in Sequoia sempervirens

Author:: L.-C. Huang; S.-Y. Pu; T. Murashige; S.-F. Fu; T.-T. Kuo; D.-D. Huang; H.-J. Huang
Source:: Biologia plantarum 2003 v.47 no.4 pp. 601-603
ISSN:: 0006-3134
Subject:: Sequoia sempervirens; Western blotting; adults; antibodies; juveniles; phosphorylation; proteins; shoots; signal transduction; tissues; tyrosine
Abstract:: ... Apical and basal halves of 3 cm long apical segments of in vitro cultured juvenile, adult and rejuvenated Sequoia sempervirens shoots were analyzed for total and tyrosine phosphorylated proteins. The latter was detected by a phosphotyrosine specific antibody. Younger tissues, or the apical halves of shoot terminals, showed larger amounts of 36, 44, 46 kDa proteins and lesser amounts of 29 kDa prot ...
DOI:: 10.1023/B:BIOP.0000041070.08300.63
: https://doi.org/10.1023/B:BIOP.0000041070.08300.63

10. Regulation of L-leucine transport in rat kidney by dexamethasone and triiodothyronine

Author:: M. Schwertfeger; K. Pissowotzki; Ch. Fleck; P. M. Taylor
Source:: Amino acids 2003 v.25 no.1 pp. 75-83
ISSN:: 0939-4451
Subject:: Western blotting; absorption; adults; density gradient centrifugation; dexamethasone; females; filtration; glucocorticoids; kidneys; messenger RNA; microvilli; rats; reverse transcriptase polymerase chain reaction; triiodothyronine
Abstract:: ... We have investigated the transport mechanisms involved in the stimulation of renal tubular reabsorption of large amino acids by glucocorticoids in vivo through the examination of activity and expression of specific transport systems L and y⁺L for L-leucine in membrane preparations of rat kidneys. Kidneys were removed from adult female Wistar rats treated with dexamethasone or triiodothyronine, and ...
DOI:: 10.1007/s00726-002-0348-y
: https://doi.org/10.1007/s00726-002-0348-y

11. Effect of daily administration of pyrantel tartrate in preventing infection in horses experimentally challenged with Sarcocystis neurona

Author:: Rossano, M.G.; Schott, H.C. II.; Kaneene, J.B.; Murphy, A.J.; Kruttlin, E.A.; Hines, M.T.; Sellon, D.C.; Patterson, J.S.; Elsheikha, H.M.; Dubey, J.P.
Source:: American journal of veterinary research 2005 v.66 no.5 pp. 846-852
ISSN:: 0002-9645
Subject:: disease detection; weanlings; pyrantel; horses; disease prevention; gender; equine protozoal myeloencephalitis; sporocysts (Protozoa); drug evaluation; Western blotting; mice; seroconversion; opossums; Sarcocystis neurona; adults
DOI:: 10.2460/ajvr.2005.66.846
PubMed:: 15934612
: https://doi.org/10.2460/ajvr.2005.66.846

12. Expression of Col1a1, Col1a2 and procollagen I in germ cells of immature and adult mouse testis

Author:: He, Zuping; Feng, Lixin; Zhang, Xiaodong; Geng, Yixun; Parodi, Daniela A; Suarez-Quian, Carlos; Dym, Martin
Source:: Reproduction 2005 v.130 no.3 pp. 333-341
ISSN:: 1470-1626
Subject:: Sertoli cells; Western blotting; adults; basement membrane; immunohistochemistry; in vitro culture; messenger RNA; mice; microarray technology; pachytene stage; reverse transcriptase polymerase chain reaction; seminiferous tubules; spermatids; spermatocytes; spermatogenesis; spermatogonia
Abstract:: ... The objective of this study was to compare the expression of Col1a1, Col1a2, and procollagen I in the seminiferous tubules of immature and adult mice and to characterize the cellular expression pattern of procollagen I in germ cells during spermatogenesis in order to provide necessary groundwork for further functional studies in the process of spermatogenesis. Microarray analysis demonstrated that ...
DOI:: 10.1530/rep.1.00694
PubMed:: 16123240
: https://doi.org/10.1530/rep.1.00694

13. In vitro translation of RNA to lactase during postnatal development of rat intestine

Author:: Jaspreet Kaur; Kamaljit Kaur; Akhtar Mahmood; Safrun Mahmood
Source:: Journal of biosciences 2005 v.30 no.2 pp. 183-189
ISSN:: 0250-5991
Subject:: Northern blotting; Western blotting; adults; antibodies; antigens; beta-galactosidase; enzyme activity; genes; intestines; messenger RNA; microvilli; molecular weight; postnatal development; pups; rats; translation (genetics); weaning
Abstract:: ... mRNA levels encoding lactase were detected by Northern blot analysis using two different probes in developing rat intestine. Probe I and probe II corresponding to second half of prolactase gene showed a 6.8 kb mRNA transcript in 7, 14, 21 and 30 day old rat intestine. There was no change in quantity of lactase mRNA detected using probe II, but hybridization with probe I showed a progressive decrea ...
DOI:: 10.1007/BF02703698
: https://doi.org/10.1007/BF02703698

14. Cloning and Characterization of a Novel, Human Cellular Retinaldehyde-binding Protein CRALBP-like (CRALBPL) Gene

Author:: Kong, Ya-Hui; Ye, Guang-Ming; Qu, Kai; Pan, Wen-Qi; Liu, Xiang-Hua; Wan, Bo; Guo, Jin-Hu; Yu, Long
Source:: Biotechnology letters 2006 v.28 no.17 pp. 1327-1333
ISSN:: 0141-5492
Subject:: Western blotting; adults; amino acids; brain; cDNA libraries; complementary DNA; genes; humans; molecular weight; open reading frames
Abstract:: ... Cellular retinaldehyde-binding protein (CRALBP) plays a role in the vertebrate visual process as a substrate-routing protein. It belongs to a widespread lipid-binding SEC14-like protein family. All the members of the family have the lipid-binding domain called CRAL-TRIO. Here we have isolated a new human CRAL-TRIO domain containing a CRALBP-like (CRALBPL) gene from the cDNA library of human adult ...
DOI:: 10.1007/s10529-006-9096-5
PubMed:: 16802092
: https://doi.org/10.1007/s10529-006-9096-5

15. Selective expression of the scaffold protein JSAP1 in spermatogonia and spermatocytes

Author:: Bayarsaikhan, Munkhuu; Shiratsuchi, Akiko; Gantulga, Davaakhuu; Nakanishi, Yoshinobu; Yoshioka, Katsuji
Source:: Reproduction 2006 v.131 no.4 pp. 711-719
ISSN:: 1470-1626
Subject:: Western blotting; adults; immunohistochemistry; mice; mitogen-activated protein kinase; postnatal development; proteins; signal transduction; somatic cells; spermatids; spermatocytes; spermatogenesis; spermatogonia; testes
Abstract:: ... Scaffold proteins of mitogen-activated protein kinase (MAPK) intracellular signal transduction pathways mediate the efficient and specific activation of the relevant MAPK signaling modules. Previously, our group and others have identified c-Jun NH₂-terminal kinase (JNK)/stress-activated protein kinase-associated protein 1 (JSAP1, also known as JNK-interacting protein 3) as a scaffold protein for J ...
DOI:: 10.1530/rep.1.00939
PubMed:: 16595722
: https://doi.org/10.1530/rep.1.00939

16. Faithful tissue-specific expression of the human chromosome 21-linked COL6A1 gene in BAC-transgenic mice

Author:: Xing, Luzhou; Salas, Martha; Lin, Chyuan-Sheng; Zigman, Warren; Silverman, Wayne; Subramaniyam, Shivakumar; Murty, Vundavalli V.; Tycko, Benjamin
Source:: Mammalian genome 2007 v.18 no.2 pp. 113-122
ISSN:: 0938-8990
Subject:: Down syndrome; Western blotting; abnormal development; adults; bacterial artificial chromosomes; chromosome mapping; collagen; enhancer elements; gene dosage; gene expression; genetically modified organisms; heart; humans; immunohistochemistry; messenger RNA; mice; phenotype; transgenes
Abstract:: ... We created transgenic mice with a bacterial artificial chromosome (BAC) containing the human COL6A1 gene. In high-copy and low-copy transgenic lines, we found correct temporal and spatial expression of COL6A1 mRNA, paralleling the expression of the murine Col6a1 gene in a panel of nine adult and four fetal organs. The only exception was the fetal lung, in which the transgene was expressed poorly c ...
DOI:: 10.1007/s00335-006-0082-y
: https://doi.org/10.1007/s00335-006-0082-y

17. Molecular characterization and serodiagnosis analysis of a novel lysophospholipase from Clonorchis sinensis

Author:: Ma, Changling; Hu, Xuchu; Hu, Fengyu; Li, Yanwen; Chen, Xiaoxiang; Zhou, Zhenwen; Lu, Fangli; Xu, Jin; Wu, Zhongdao; Yu, Xinbing
Source:: Parasitology research 2007 v.101 no.2 pp. 419-425
ISSN:: 0932-0113
Subject:: Clonorchis sinensis; Escherichia coli; Western blotting; adults; antigens; cDNA libraries; complementary DNA; enzyme activity; enzyme-linked immunosorbent assay; immunoglobulins; lysophosphatidylcholine; lysophospholipase; molecular weight; parasites; patients; schistosomiasis; serodiagnosis
Abstract:: ... A cDNA clone encoding a novel lysophospholipase with a predicted molecular weight of 25.2 kDa was isolated from a Clonorchis sinensis adult cDNA library. The enzyme activity of the recombinant protein expressed in Escherichia coli was determined using phosphatidylcholine and lysophosphatidylcholine as substrates. Western blotting analysis indicated that it belonged to excretory/secretory proteins ...
DOI:: 10.1007/s00436-007-0481-3
PubMed:: 17318582
: https://doi.org/10.1007/s00436-007-0481-3

18. Preliminary characterization of an adult worm “vomit” preparation of Schistosoma mansoni and its potential use as antigen for diagnosis

Author:: Planchart, Sandra; Incani, Renzo Nino; Cesari, Italo Mario
Source:: Parasitology research 2007 v.101 no.2 pp. 301-309
ISSN:: 0932-0113
Subject:: temperature; chemical composition; drug therapy; antigens; humans; Schistosoma mansoni; drugs; parasitoses; chromatography; Western blotting; digestion; mice; adults; people; enzyme activity; blood; digestive system; alkaline phosphatase; cysteine proteinases
Abstract:: ... Schistosoma mansoni is a parasitic trematode of the portal-mesenteric veins with a closed-end intestine. Adult worms regurgitate their intestinal content after digestion, together with constituents of the lining gut. Some of these molecules circulate in the blood and are antigenic. We obtain a “vomit” preparation and preliminary evaluate its biochemical composition and antigenic capacity. The “vom ...
DOI:: 10.1007/s00436-007-0482-2
PubMed:: 17310394
: https://doi.org/10.1007/s00436-007-0482-2

19. Proline-rich Gla protein 2 is a cell-surface vitamin K-dependent protein that binds to the transcriptional coactivator Yes-associated protein

Author:: Kulman, John D.; Harris, Jeff E.; Xie, Ling; Davie, Earl W.
Source:: Proceedings of the National Academy of Sciences of the United States of America 2007 v.104 no.21 pp. 8767-8772
ISSN:: 0027-8424
Subject:: Western blotting; adults; antagonists; carboxylation; flow cytometry; gamma-carboxyglutamic acid; mammals; proteins; signal transduction; therapeutics; tissues; transcription (genetics); two hybrid system techniques; vitamin K; warfarin
Abstract:: ... Proline-rich Gla protein 2 (PRGP2) is one of four known vertebrate transmembrane γ-carboxyglutamic acid (Gla) proteins. Members of this protein family are broadly expressed in fetal and adult human tissues and share a common architecture consisting of a predicted propeptide and Gla domain, a single-pass transmembrane segment, and tandem Pro/Leu-Pro-Xaa-Tyr (PY) motifs near their C termini. Using a ...
DOI:: 10.1073/pnas.0703195104
PubMed:: 17502622
PubMed Central:: PMC1885577
: https://doi.org/10.1073/pnas.0703195104

20. Thermally induced and developmentally regulated expression of a small heat shock protein in Trichinella spiralis

Author:: Wu, Z.; Nagano, I.; Boonmars, T.; Takahashi, Y.
Source:: Parasitology research 2007 v.101 no.1 pp. 201-212
ISSN:: 0932-0113
Subject:: Trichinella spiralis; Western blotting; adults; amino acids; citrate (si)-synthase; cold; cold stress; complementary DNA; esophagus; genes; heat shock proteins; heat stress; integument; muscle larvae; myocytes; neonates; sequence analysis
Abstract:: ... A cDNA encoding a small heat shock protein of Trichinella spiralis, Ts-sHsp, was cloned and expressed and is herein characterized. This cDNA encoded a predicted protein of 165 amino acids, which had a high sequence identity in α crystallin domain with various small heat shock proteins of other organisms. A Western blot analysis indicated that anti-Ts-sHsp recombinant antibody recognized the protei ...
DOI:: 10.1007/s00436-007-0462-6
PubMed:: 17268805
: https://doi.org/10.1007/s00436-007-0462-6

21. ArHsp22, a developmentally regulated small heat shock protein produced in diapause-destined Artemia embryos, is stress inducible in adults

Author:: Qiu, Zhijun; MacRae, Thomas H.
Source:: FEBS journal 2008 v.275 no.14 pp. 3556-3566
ISSN:: 1742-464X
Subject:: Artemia franciscana; Western blotting; adults; arginine; citrate (si)-synthase; denaturation; diapause; gene expression; heat shock proteins; insulin; quantitative polymerase chain reaction; stress tolerance; thermal stress
Abstract:: ... Diapause embryos of the crustacean Artemia franciscana exhibit extreme stress tolerance, a property thought to involve molecular chaperones known as small heat shock proteins. To further explore this idea, the structure, function and synthesis of ArHsp22, an Artemia small heat shock protein, were characterized. ArHsp22 contains amino-terminal WXDPF motifs, an α-crystallin domain with a highly cons ...
DOI:: 10.1111/j.1742-4658.2008.06501.x
: https://doi.org/10.1111/j.1742-4658.2008.06501.x

22. CCDC134, a novel secretory protein, inhibits activation of ERK and JNK, but not p38 MAPK

Author:: Huang, J.; Shi, T.; Ma, T.; Zhang, Y.; Ma, X.; Lu, Y.; Song, Q.; Liu, W.; Ma, D.; Qiu, X.
Source:: Cellular and molecular life sciences 2008 v.65 no.2 pp. 338-349
ISSN:: 1420-682X
Subject:: Northern blotting; Western blotting; adults; amino acids; genes; humans; immunohistochemistry; messenger RNA; mitogen-activated protein kinase; nucleotides; phosphorylation; reverse transcriptase polymerase chain reaction; small interfering RNA; tissues; transcription (genetics)
Abstract:: ... In this study, we report a novel gene, CCDC134 (coiled-coil domain containing 134), that encodes a secretory protein that can inhibit the MAPK pathway as a novel human MAPK-regulating protein. The CCDC134 mRNA contains 1280 nucleotides, encoding a protein of 229 amino acids. CCDC134 is a classical secretory protein. Expression profile analysis by Northern blot, RT-PCR, immunohistochemistry and Wes ...
DOI:: 10.1007/s00018-007-7448-5
: https://doi.org/10.1007/s00018-007-7448-5

23. Cloning and characterization of a cDNA fragment encoding a Schistosoma mansoni actin-binding protein (Smfilamin)

Author:: M. R. Mohamed; K. A. Shalaby; P. T. LoVerde; N. M. Abd Allah; A. M. Karim
Source:: Parasitology research 2008 v.102 no.5 pp. 1035-1042
ISSN:: 0932-0113
Subject:: Northern blotting; Schistosoma mansoni; Western blotting; adults; amino acids; antiserum; cDNA libraries; clones; complementary DNA; filamin; fluorescence; fluorescent antibody technique; genes; humans; immunoglobulin G; rabbits; reverse transcriptase polymerase chain reaction; schistosomula
Abstract:: ... To identify vaccine candidates for Schistosoma mansoni, the IgG fraction of rabbit antiserum raised against immature female worms affinity purified over a NP-40 extract of 3-h schistosomula was used to immunoscreen a cercarial λgt11 cDNA library. One clone with a 1.5-kb cDNA insert revealed an encoded peptide of 479 amino acids, which bears homology to human actin-binding protein (ABP-280 = filami ...
DOI:: 10.1007/s00436-007-0872-5
PubMed:: 18283496
: https://doi.org/10.1007/s00436-007-0872-5

24. Essential Role of Chitinase in the Development of the Filarial Nematode Acanthocheilonema viteae

Author:: Tachu, Babila; Pillai, Smitha; Lucius, Richard; Pogonka, Thomas
Source:: Infection and immunity 2008 v.76 no.1 pp. 221-228
ISSN:: 0019-9567
Subject:: RNA interference; Western blotting; genes; adults; Southern blotting; clones; hatching; pathogens; DNA libraries; complementary DNA; Acanthocheilonema viteae; double-stranded RNA; Northern blotting; quantitative polymerase chain reaction; microfilariae; vaccines; genomics; molting; death; rodents; chitinase
Abstract:: ... Chitinases of pathogens have been proposed as potential targets of vaccines or specific inhibitors. We studied the genomic organization, transcript levels, developmental expression, and biological function of chitinases in the rodent filarial nematode Acanthocheilonema viteae, a model organism for human-pathogenic filarial worms. Characterization of nine genomic clones from an A. viteae phage libr ...
DOI:: 10.1128/IAI.00701-07
PubMed:: 17938220
PubMed Central:: PMC2223640
: https://doi.org/10.1128/IAI.00701-07

25. Foot-shock Stress-Induced Regional Iron Accumulation and Altered Iron Homeostatic Mechanisms in Rat Brain

Author:: Ma, Long; Wang, Wanyin; Zhao, Min; Li, Min
Source:: Biological trace element research 2008 v.126 no.1-3 pp. 204-213
ISSN:: 0163-4984
Subject:: Western blotting; adults; atomic absorption spectrometry; blood serum; cortex; ferritin; hippocampus; homeostasis; iron; lactoferrin; liver; models; rats; transferrin
Abstract:: ... Like in other organs, iron in the brain plays an important role in various biological processes. Previous studies have shown that systemic iron homeostasis in mammalians was changed under specific stress conditions. The present study aimed to investigate effects of stress on brain iron homeostasis in rats using a foot-shock stress model. Young adult male Sprague-Dawley rats were randomly assigned ...
DOI:: 10.1007/s12011-008-8197-5
PubMed:: 18709494
: https://doi.org/10.1007/s12011-008-8197-5

26. In Vitro Induction of 60-kDa and 70-kDa Heat Shock Proteins by Endosulphan and Monocrotophos in Sheep Blowfly Lucilia cuprina

Author:: Sharma, Sunita; Rohilla, Manoj Singh; Reddy, P. V. J.; Tiwari, P. K.
Source:: Archives of environmental contamination and toxicology 2008 v.55 no.1 pp. 57-69
ISSN:: 0090-4341
Subject:: Lucilia cuprina; Western blotting; adults; biomarkers; digestive system; heat shock proteins; larvae; monitoring; monocrotophos; stress response; toxicity
Abstract:: ... The effect of two most commonly used and highly toxic organic pesticides, endosulphan (organochlorine) and monocrotophos (organophosphate), was studied in a blowfly, Lucilia cuprina, to test whether these pesticides induce the stress response and, if so, whether the intensity of the response, in terms of induction of heat shock proteins (HSPs), HSP60 and HSP70 in particular, is pesticide concentra ...
DOI:: 10.1007/s00244-007-9093-2
: https://doi.org/10.1007/s00244-007-9093-2

27. Molecular Characterization and Expression Pattern of Fetuin-B in Gibel Carp (Carassius auratus gibelio)

Author:: Liu, Jing-Xia; Zhai, Yan-Hua; Geng, Fan-Suo; Xia, Jian-Hong; Gui, Jian-Fang
Source:: Biochemical genetics 2008 v.46 no.9-10 pp. 620-633
ISSN:: 0006-2928
Subject:: Carassius auratus; Western blotting; adults; brain; carp; embryogenesis; hepatocytes; kidneys; larvae; liver; organogenesis; reverse transcriptase polymerase chain reaction
Abstract:: ... Fetuin-B has recently been cloned and identified from rats, mice, and humans; their expression patterns, however, have not been elucidated yet. In this study, Cagfetuin-B has been cloned in gibel carp. RT-PCR and Western blot detection revealed that Cagfetuin-B is first transcribed from the blastula stage and at a relatively stable level afterward during embryogenesis and the larval stage. Cagfetu ...
DOI:: 10.1007/s10528-008-9176-4
: https://doi.org/10.1007/s10528-008-9176-4

28. Recognition and characterization of TGF-β receptor interacting protein 1 (TRIP-1) containing WD40 repeats from Clonorchis sinensis by bioinformatics, cloning, and expression in Escherichia coli

Author:: Hu, Xuchu; Zhou, Hongjuan; Hu, Fengyu; Xu, Jin; Zhao, Yuli; Yu, Xinbing
Source:: Parasitology research 2008 v.103 no.5 pp. 1151-1158
ISSN:: 0932-0113
Subject:: Clonorchis sinensis; Escherichia coli; Western blotting; adults; antigens; bioinformatics; complementary DNA; eukaryotic cells; messenger RNA; parasites; pathogenesis; patients; phosphorylation; plasmids; ribosomes; transforming growth factor beta; translation (genetics)
Abstract:: ... A cDNA clone encoding a homologue of transforming growth factor beta (TGF-β) receptor interacting protein 1 (TRIP-1) was recognized and isolated from full-length cDNA plasmid library of Clonorchis sinensis adult. TRIP-1 is a bifunctional molecule in all eukaryote, which modulates the signaling pathway of TGF-β as a phosphorylation substrate of TGF-β type II receptor kinase and controls ribosome as ...
DOI:: 10.1007/s00436-008-1109-y
: https://doi.org/10.1007/s00436-008-1109-y

29. The secretome of the filarial parasite, Brugia malayi: Proteomic profile of adult excretory-secretory products

Author:: Hewitson, James P.; Harcus, Yvonne M.; Curwen, Rachel S.; Dowle, Adam A.; Atmadja, Agnes K.; Ashton, Peter D.; Wilson, Alan; Maizels, Rick M.
Source:: Molecular and biochemical parasitology 2008 v.160 no.1 pp. 8-21
ISSN:: 0166-6851
Subject:: Acanthocheilonema viteae; Brugia malayi; Western blotting; adults; endosymbionts; excretory-secretory products; filariasis; fructose-bisphosphate aldolase; genome; glutathione peroxidase; glycolysis; glycoproteins; humans; immune response; leucyl aminopeptidase; macrophage migration inhibitory factors; monoclonal antibodies; parasites; phenotype; proteomics; triose-phosphate isomerase; two-dimensional gel electrophoresis
Abstract:: ... The secretome of a parasite in its definitive host can be considered to be its genome in trans, to the extent that secreted products encoded by the parasite fulfill their function in the host milieu. The 'extended phenotype' of the filarial parasite, Brugia malayi, is of particular interest because of the evidence that infection results in potent down-modulation of the host immune response. We col ...
DOI:: 10.1016/j.molbiopara.2008.02.007
: https://doi.org/10.1016/j.molbiopara.2008.02.007

30. Amphibian larvae and zinc sulphate: a suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium

Author:: Yovanovich, Carola A. M.; Jungblut, Lucas D.; Heer, Tamara; Pozzi, Andrea G.; Paz, Dante A.
Source:: Cell and tissue research 2009 v.336 no.1 pp. 1-9
ISSN:: 0302-766X
Subject:: Western blotting; adults; amphibians; antiserum; epithelium; experimental design; image analysis; immunohistochemistry; models; neurons; tadpoles; zinc sulfate
Abstract:: ... The vertebrate olfactory system has fascinated neurobiologists over the last six decades because of its ability to replace its neurons and synaptic connections continuously throughout adult life, under both physiological and pathological conditions. Among the factors that are proposed to be involved in this regenerative potential, brain-derived neurotrophic factor (BDNF) is a candidate for having ...
DOI:: 10.1007/s00441-009-0752-7
PubMed:: 19221803
: https://doi.org/10.1007/s00441-009-0752-7

31. Analysis of OCT4 expression in an extended panel of human tumor cell lines from multiple entities and in human mesenchymal stem cells

Author:: Mueller, T.; Luetzkendorf, J.; Nerger, K.; Schmoll, H.-J.; Mueller, L. P.
Source:: Cellular and molecular life sciences 2009 v.66 no.3 pp. 495-503
ISSN:: 1420-682X
Subject:: Western blotting; adults; embryonic stem cells; germ cells; humans; immunocytochemistry; immunohistochemistry; neoplasm cells; reverse transcriptase polymerase chain reaction
Abstract:: ... OCT4 is considered a main regulator of embryonic stem cell pluripotency and self renewal capacity. It was shown that relevant OCT4 expression only occurs in cells of embryonic pluripotent nature. However, several recent publications claimed to have demonstrated OCT4 expression in human somatic tumor cells, human adult stem or progenitor cells and differentiated cells.We analysed 42 human tumor cel ...
DOI:: 10.1007/s00018-008-8623-z
: https://doi.org/10.1007/s00018-008-8623-z

32. Angiogenic properties of adult human thymus fat

Author:: Salas, Julián; Montiel, Mercedes; Jiménez, Eugenio; Valenzuela, Miguel; Valderrama, José Francisco; Castillo, Rafael; González, Sergio; El Bekay, Rajaa
Source:: Cell and tissue research 2009 v.338 no.2 pp. 313-318
ISSN:: 0302-766X
Subject:: Western blotting; adipose tissue; adults; angiogenesis; cell movement; endothelial cells; genes; humans; immunohistochemistry; lipogenesis; protein synthesis; proteins; quantitative polymerase chain reaction; umbilical cord; vascular endothelial growth factor A
Abstract:: ... The endogenous proangiogenic properties of adipose tissue are well recognized. Although the adult human thymus has long been known to degenerate into fat tissue, it has never been considered as a potential source of angiogenic factors. We have investigated the expression of diverse angiogenic factors, including vascular endothelial growth factor A and B, angiopoietin 1, and tyrosine-protein kinase ...
DOI:: 10.1007/s00441-009-0867-x
PubMed:: 19760288
: https://doi.org/10.1007/s00441-009-0867-x

33. Characterization of phosphodiesterase-5 as a surface protein in the tegument of Schistosoma mansoni

Author:: Rofatto, Henrique K.; Tararam, Cibele A.; Borges, William C.; Wilson, R. Alan; Leite, Luciana C.C.; Farias, Leonardo P.
Source:: Molecular and biochemical parasitology 2009 v.166 no.1 pp. 32-41
ISSN:: 0166-6851
Subject:: Schistosoma mansoni; Western blotting; adults; antibodies; cercariae; developing countries; disease control; enzyme activity; gene expression regulation; hydrolysis; mammals; proteomics; reverse transcriptase polymerase chain reaction; schistosomiasis; signal peptide; surface proteins; vaccines
Abstract:: ... Schistosoma mansoni is a major causative agent of schistosomiasis, an important parasitic disease that constitutes a severe health problem in developing countries. Even though an effective treatment exists, it does not prevent re-infection and the development of an effective vaccine still remains the most desirable means of control for this disease. In this work we describe the cloning and charact ...
DOI:: 10.1016/j.molbiopara.2009.02.006
: https://doi.org/10.1016/j.molbiopara.2009.02.006

34. Cloning, expression and functional analysis of a general odorant-binding protein 2 gene of the rice striped stem borer, Chilo suppressalis (Walker) (Lepidoptera: Pyralidae)

Author:: Gong, Z.-J.; Zhou, W.-W.; Yu, H.-Z.; Mao, C.-G.; Zhang, C.-X.; Cheng, J.A.; Zhu, Z.-R.
Source:: Insect molecular biology 2009 v.18 no.3 pp. 405-417
ISSN:: 0962-1075
Subject:: Chilo suppressalis; Escherichia coli; Pyralidae; Western blotting; adults; affinity chromatography; amino acids; antennae; benzaldehyde; binding proteins; complementary DNA; females; fluorescence; gel chromatography; genes; males; messenger RNA; odor compounds; open reading frames; pheromones; rapid amplification of cDNA ends; reverse transcriptase polymerase chain reaction; rice
Abstract:: ... A full-length cDNA encoding a general odorant binding protein 2 (GOBP2) was cloned from the antennae of the rice striped stem borer, Chilo suppressalis (Lepidoptera: Pyralidae), by the combination of reverse transcription PCR (RT-PCR) and rapid amplification of cDNA ends PCR (RACE-PCR). The cDNA contains a 489 bp open reading frame, which encodes a 162 amino acid protein, termed as Ch. suppressali ...
DOI:: 10.1111/j.1365-2583.2009.00886.x
PubMed:: 19523072
: https://doi.org/10.1111/j.1365-2583.2009.00886.x

35. Cloning, molecular characterization of a 13-kDa antigen from Schistosoma japonicum, Sj13, a putative salivary diagnosis candidate for Schistosomiasis japonica

Author:: Zhou, Yan-ping; Wu, Zhong-dao; Yang, Lin-lin; Sun, Xi; You, Xu; Yu, Xin-bing; Hu, Wei; Zheng, Huan-qin; Lv, Zhi-yue
Source:: Parasitology research 2009 v.105 no.5 pp. 1435-1444
ISSN:: 0932-0113
Subject:: Escherichia coli; Schistosoma japonicum; Western blotting; adults; amino acid sequences; amino acids; antibodies; antibody detection; antigens; cDNA libraries; clones; complementary DNA; databases; egg shell; eggs; enzyme-linked immunosorbent assay; epidemiological studies; immunoglobulin G; infectious diseases; molecular weight; open reading frames; patients; reverse transcriptase polymerase chain reaction; saliva
Abstract:: ... Saliva has been suggested as an easily accessible and a noninvasive diagnostic alternative for detection of antibodies. To identify and characterize Schistosoma japonicum (S. japonicum) antigens that are recognized by saliva of infected host, we have used a pool of saliva from infected patients to immunoscreen an egg cDNA library of S. japonicum. The open reading frame of the isolated two clones e ...
DOI:: 10.1007/s00436-009-1575-x
PubMed:: 19639340
: https://doi.org/10.1007/s00436-009-1575-x

36. Differential localization of α' and β subunits of protein kinase CK2 during rat spermatogenesis

Author:: Alvarado-Díaz, Carlos P.; Tapia, Julio C.; Antonelli, Marcelo; Moreno, Ricardo D.
Source:: Cell and tissue research 2009 v.338 no.1 pp. 139-149
ISSN:: 0302-766X
Subject:: Sertoli cells; Western blotting; acrosome; adulthood; adults; apoptosis; embryogenesis; flagellum; humans; immunohistochemistry; meiosis; protein kinases; protein subunits; rats; serine; spermatocytes; spermatogenesis; spermatogonia; threonine; yeasts
Abstract:: ... Protein kinase CK2 is a serine/threonine kinase expressed in organisms from yeast to human and is composed of a catalytic subunit (α or α') and a regulatory subunit (β) forming a holoenzyme with the possible subunit combinations α₂β₂, α'₂β₂, or αα'β₂. This kinase has been shown to be involved in embryonic development and gametogenesis. We have studied the expression of the CK2α' and CK2β subunits ...
DOI:: 10.1007/s00441-009-0847-1
: https://doi.org/10.1007/s00441-009-0847-1

37. Expression and characterization of aspartic protease gene in eggs and larvae stage of Ancylostoma caninum

Author:: Yang, Yurong; Wei, Hua; Qin, Weiwen; Zheng, Jing
Source:: Parasitology research 2009 v.104 no.6 pp. 1327-1333
ISSN:: 0932-0113
Subject:: Ancylostoma caninum; Escherichia coli; Western blotting; adults; antiserum; aspartic proteinases; developing countries; digestion; eggs; gelatin; genes; hookworms; immunoassays; larvae; messenger RNA; public health; reverse transcriptase polymerase chain reaction
Abstract:: ... Hookworm infection is still a public health problem in developing countries. Aspartic protease plays an important role in parasite invasion and migration in the host. Aspartic protease gene from Ancylostoma caninum has been reported (Biochem Biophys Res Commun 227:294-302, 1996), but the activity of Acasp in eggs and larvae stage has not been studied. This paper reported the cloning of Acasp, expr ...
DOI:: 10.1007/s00436-009-1332-1
PubMed:: 19169710
: https://doi.org/10.1007/s00436-009-1332-1

38. Expression profile, localization of an 8-kDa calcium-binding protein from Schistosoma japonicum (SjCa8), and vaccine potential of recombinant SjCa8 (rSjCa8) against infections in mice

Author:: Lv, Zhi-yue; Yang, Lin-lin; Hu, Shao-min; Sun, Xi; He, Han-jiang; He, Si-jie; Li, Zheng-yu; Zhou, Yan-ping; Fung, Ming-Chiu; Yu, Xin-bing; Zheng, Huan-qin; Cao, Ai-lian; Wu, Zhong-dao
Source:: Parasitology research 2009 v.104 no.4 pp. 733-743
ISSN:: 0932-0113
Subject:: Schistosoma japonicum; Western blotting; adults; calcium; cercariae; desorption; dialysis; digestion; eggs; genes; immune response; ionization; mice; permeability; protective effect; reverse transcriptase polymerase chain reaction; spectrometers; tail; thrombin; vaccination
Abstract:: ... Researches on genes expressed in a cercarial stage-specific manner may help us understand the molecular events and functions during schistosome invasion of skin. A genomic clone encoding 8-kDa calcium-binding protein (SjCa8) specifically expressed in cercariae and skin-stage schistosomulum (transformed within 3 h) was obtained from cercariae. Recombinant protein was expressed in vector pET32a (+) ...
DOI:: 10.1007/s00436-008-1249-0
: https://doi.org/10.1007/s00436-008-1249-0

39. Fat diet affects leptin receptor levels in the rat cerebellum

Author:: Koros, Christos; Boukouvalas, George; Gerozissis, Kyriaki; Kitraki, Efthimia
Source:: Nutrition 2009 v.25 no.1 pp. 85-87
ISSN:: 0899-9007
Subject:: metabolic syndrome; high fat diet; leptin; nutrition-genotype interaction; puberty; hormone receptors; weaning; gender differences; Western blotting; sexual dimorphism; energy expenditure; animal models; cerebellum; rats; postpartum period; adults; adolescence
Abstract:: ... Objective: The role of leptin receptors (Ob-Rs) within the hypothalamus in the control of energy expenditure has well been established. However, their role and regulation in other brain areas, including the cerebellum, is largely unexplored. In the present study we examined whether Ob-R levels in the rat cerebellum are influenced by a high-fat diet and if these changes are sexually divergent durin ...
DOI:: 10.1016/j.nut.2008.06.033
: https://doi.org/10.1016/j.nut.2008.06.033

40. Glycotope analysis in miracidia and primary sporocysts of Schistosoma mansoni: Differential expression during the miracidium-to-sporocyst transformation

Author:: Peterson, Nathan A.; Hokke, Cornelis H.; Deelder, André M.; Yoshino, Timothy P.
Source:: International journal for parasitology 2009 v.39 no.12 pp. 1331-1344
ISSN:: 0020-7519
Subject:: Biomphalaria glabrata; Schistosoma mansoni; Western blotting; adults; confocal laser scanning microscopy; epitopes; fluorescence microscopy; gene expression regulation; glycoproteins; glycosylation; hosts; immune response; larvae; miracidia; monoclonal antibodies; parasites; snails; somatic cells
Abstract:: ... Fucosylated carbohydrate epitopes (glycotopes) expressed by larval and adult schistosomes are thought to modulate the host immune response and possibly mediate parasite evasion in intermediate and definitive hosts. While previous studies showed glycotope expression is developmentally and stage-specifically regulated, relatively little is known regarding their occurrence in miracidia and primary sp ...
DOI:: 10.1016/j.ijpara.2009.06.002
PubMed:: 19545571
PubMed Central:: PMC3740939
: https://doi.org/10.1016/j.ijpara.2009.06.002

41. Identification and characterization of antimicrobial peptide, defensin, in the taiga tick, Ixodes persulcatus

Author:: Saito, Y.; Konnai, S.; Yamada, S.; Imamura, S.; Nishikado, H.; Ito, T.; Onuma, M.; Ohashi, K.
Source:: Insect molecular biology 2009 v.18 no.4 pp. 531-539
ISSN:: 0962-1075
Subject:: Bacillus subtilis; Corynebacterium renale; Escherichia coli O157; Gram-negative bacteria; Gram-positive bacteria; Ixodes persulcatus; Staphylococcus aureus; Western blotting; adults; amino acid sequences; antibacterial properties; antimicrobial peptides; blood meal; cDNA libraries; complementary DNA; females; humans; messenger RNA; midgut; nymphs; salivary glands; synthetic peptides; taiga; tick-borne diseases; ticks; Japan
Abstract:: ... Ixodes persulcatus is the primary vector for human tick-borne diseases in Japan. A cDNA library was constructed from whole body homogenates of fed nymphs of I. persulcatus. From this library, one cDNA encoding defensin-like antimicrobial peptide was identified. The amino-acid sequence showed high similarity to those of the defensins of other ticks and arthropods. I. persulcatus defensin mRNA trans ...
DOI:: 10.1111/j.1365-2583.2009.00897.x
PubMed:: 19604312
: https://doi.org/10.1111/j.1365-2583.2009.00897.x

42. Isolation and immunolocalization of a putative protective antigen (p26/23) from adult Haemonchus contortus

Author:: García-Coiradas, L.; Angulo-Cubillán, F.; Méndez, S.; Larraga, V.; de la Fuente, C.; Cuquerella, M.; Alunda, J. M.
Source:: Parasitology research 2009 v.104 no.2 pp. 363-369
ISSN:: 0932-0113
Subject:: Haemonchus contortus; Western blotting; adults; affinity chromatography; amino acid sequences; amino acids; antigens; helminths; lambs; larvae; molecular weight; nylon; polyacrylamide gel electrophoresis; proteinase inhibitors; proteins; sodium dodecyl sulfate
Abstract:: ... The putative protective antigen, p26/23, from adult Haemonchus contortus was isolated. A soluble extract from adult helminths obtained from the abomasa of hyperinfected (12,000 infective larvae) female Manchego lambs and treated with a mixture of protease inhibitors was subjected to affinity chromatography (hexylgluthatione) to eliminate the enzyme gluthatione S-transferase. The eluate was analyze ...
DOI:: 10.1007/s00436-008-1204-0
PubMed:: 18825413
: https://doi.org/10.1007/s00436-008-1204-0

43. Molecular characterization of a novel Clonorchis sinensis secretory phospholipase A₂ and investigation of its potential contribution to hepatic fibrosis

Author:: Hu, Fengyu; Hu, Xuchu; Ma, Changling; Zhao, Junhong; Xu, Jin; Yu, Xinbing
Source:: Molecular and biochemical parasitology 2009 v.167 no.2 pp. 127-134
ISSN:: 0166-6851
Subject:: Clonorchis sinensis; Escherichia coli; Western blotting; active sites; adults; calcium; cell cycle; cell membranes; collagen; complementary DNA; enzyme activity; fibrosis; fluorescent antibody technique; gene expression; gene expression regulation; genes; membrane proteins; phosphatidylcholines; phospholipase A2; plasmids; polyacrylamide gel electrophoresis; prediction; receptors; recombinant proteins; reverse transcriptase polymerase chain reaction; signal peptide
Abstract:: ... A gene encoding a homologue of phospholipase A₂ was identified from the Clonorchis sinensis adult cDNA plasmid library. The deduced amino acid sequence including a signal peptide that has 28-46% identity with secretory phospholipase A₂, group III (group III sPLA₂) of other species. It also has typical features of group III sPLA₂s including 10 cysteines, the key residues of the Ca²⁺ loop and cataly ...
DOI:: 10.1016/j.molbiopara.2009.05.003
: https://doi.org/10.1016/j.molbiopara.2009.05.003

44. Molecular cloning and analysis of stage and tissue-specific expression of Cathepsin L-like protease from Clonorchis sinensis

Author:: Li, Yanwen; Hu, Xuchu; Liu, Xiaoquan; Xu, Jing; Hu, Fengyu; Ma, Changling; Yu, Xinbing
Source:: Parasitology research 2009 v.105 no.2 pp. 447-452
ISSN:: 0932-0113
Subject:: Clonorchis sinensis; Escherichia coli; Western blotting; adults; antigens; bioinformatics; cathepsin L; complementary DNA; denaturation; digestion; drugs; excretory-secretory products; excystation; fish; foods; humans; molecular cloning; parasites; reverse transcriptase polymerase chain reaction; serodiagnosis; signal peptide
Abstract:: ... Cathepsin L of parasite plays multiple roles in growth, food uptake, and invasion into host and pathogenesis, which makes it a valuable target for diagnosis, vaccine, and drug. In this study, we identified a cDNA encoding cathepsin L homolog (CsCPL) from the library of Clonorchis sinensis adult by bioinformatics analysis. Sequence encoding proenzyme of CsCPL (removal of signal peptide, CsproCPL) w ...
DOI:: 10.1007/s00436-009-1406-0
PubMed:: 19308452
: https://doi.org/10.1007/s00436-009-1406-0

45. The functional expression and characterisation of a cysteine peptidase from the invasive stage of the neuropathogenic schistosome Trichobilharzia regenti

Author:: Dolečková, Kateřina; Kašný, Martin; Mikeš, Libor; Cartwright, Jared; Jedelský, Petr; Schneider, Eric L.; Dvořák, Jan; Mountford, Adrian P.; Craik, Charles S.; Horák, Petr
Source:: International journal for parasitology 2009 v.39 no.2 pp. 201-211
ISSN:: 0020-7519
Subject:: parasites; Trichobilharzia; Komagataella pastoris; elastase; serine; Western blotting; genes; adults; cercariae; cross reaction; antibodies; cathepsin B; antiserum; cysteine; blood serum; humans; yeasts; schistosomula; transcription (genetics); birds; proteins; Schistosoma haematobium; Schistosoma japonicum; Schistosoma mansoni; pH; sequence homology
Abstract:: ... A transcriptional product of a gene encoding cathepsin B-like peptidase in the bird schistosome Trichobilharzia regenti was identified and cloned. The enzyme was named TrCB2 due to its 77% sequence similarity to cathepsin B2 from the important human parasite Schistosoma mansoni. The zymogen was expressed in the methylotropic yeast Pichia pastoris; procathepsin B2 underwent self-processing in yeast ...
DOI:: 10.1016/j.ijpara.2008.06.010
PubMed:: 18708063
PubMed Central:: PMC2625449
: https://doi.org/10.1016/j.ijpara.2008.06.010

46. The ubiquitin-proteasome system in Strongyloididae. Biochemical evidence for developmentally regulated proteolysis in Strongyloides venezuelensis

Author:: de Paula, Fabiana M.; Castro-Borges, William; Júnior, Olavo S. Pereira; de Souza Gomes, Matheus; Ueta, Marlene T.; Rodrigues, Vanderlei
Source:: Parasitology research 2009 v.105 no.2 pp. 567-576
ISSN:: 0932-0113
Subject:: Strongyloides; Western blotting; adults; expressed sequence tags; humans; hydrolysis; intestinal mucosa; larvae; parasites; pathogens; proteasome endopeptidase complex; proteolysis; synthetic peptides
Abstract:: ... Nematode parasites from the genus Strongyloides spp. are important pathogens of the intestinal mucosa of animals and humans. Their complex life cycles involve alternating developmental adaptations between larvae stages and the adult parthenogenetic female. Here, we report, primarily through homology-based searching, the existence of the major components of the ubiquitin-proteasome system in this g ...
DOI:: 10.1007/s00436-009-1430-0
PubMed:: 19352707
: https://doi.org/10.1007/s00436-009-1430-0

47. Weak effect of trans-10, cis-12-conjugated linoleic acid on body fat accumulation in adult hamsters

Author:: Miranda, Jonatan; Churruca, Itziar; Fernández-Quintela, Alfredo; Rodríguez, Victor Manuel; Macarulla, María Teresa; Simón, Edurne; Portillo, María Puy
Source:: British journal of nutrition 2009 v.102 no.11 pp. 1583-1589
ISSN:: 0007-1145
Subject:: hamsters; lipoprotein lipase; fatty-acid synthase; adipose tissue; reverse transcriptase polymerase chain reaction; Western blotting; lipogenesis; animal models; acetyl-CoA carboxylase; heat production; conjugated linoleic acid; spectroscopy; adults; body fat
Abstract:: ... It has been proposed that young animals and subjects are more responsive to conjugated linoleic acid (CLA) than the adults. Nevertheless, there is very little information concerning the effectiveness of CLA in adult animals. In the present study we aimed to explore the effects of trans-10, cis-12-CLA on body fat accumulation in adult hamsters, as well as on some of the molecular mechanisms describ ...
DOI:: 10.1017/S0007114509990912
PubMed:: 19619365
: https://doi.org/10.1017/S0007114509990912

48. Aquaporin AQP11 in the testis: molecular identity and association with the processing of residual cytoplasm of elongated spermatids

Author:: Yeung, C.H.; Cooper, T.G.
Source:: Reproduction 2010 v.139 no.1 pp. 209-216
ISSN:: 1470-1626
Subject:: Sertoli cells; Western blotting; adults; aquaporins; databases; gene banks; gene expression; humans; immunohistochemistry; kidneys; messenger RNA; mice; nucleotides; open reading frames; organelles; polymerase chain reaction; protein synthesis; rats; spermatids; spermatogenesis; spermatozoa; spermiation; tail
Abstract:: ... AQP11 is one of the latest aquaporin (AQP) family members found, which differs from the other AQPs by its intracellular localisation and unusual water pore nucleotides with unclear function. Despite the highest mRNA expression among organs having been reported in the testis, the testicular molecule has not been studied in detail. Immunohistochemistry of rat adult testis localised AQP11 to the elon ...
DOI:: 10.1530/REP-09-0298
PubMed:: 19812234
: https://doi.org/10.1530/REP-09-0298

49. Effect of RH-2485 on development, metamorphosis and synthesis of major proteins in female silkworm Bombyx mori

Author:: Ayyamperumal Rajathi; Jeyaraj Pandiarajan; Muthukalingan Krishnan
Source:: Biologia 2010 v.65 no.5 pp. 903-913
ISSN:: 1336-9563
Subject:: moths; pupae; silk; polyacrylamide gel electrophoresis; silkworms; Western blotting; hemolymph; methoxyfenozide; adults; sublethal effects; fat body; choriogenesis; vitellogenin; females; storage proteins; vitellogenesis; wings; insect pests; lethal dose 50; probit analysis; models; pupation; larvae; eggs; oocytes; mortality; abdomen; instars; spinning; ovarian development; Bombyx mori; egg production
Abstract:: ... Toxicological data on silkworm Bombyx mori are quite comparable to those of other lepidopteran pest insects, therefore, it is considered as a suitable model for exploring effects of any new synthetic formulations. In this study, female V instar larvae of silk moth B. mori were chosen to evaluate the lethal and sublethal toxicity effects of RH-2485 (methoxyfenozide), a non-steroidal ecdysteroid ago ...
DOI:: 10.2478/s11756-010-0104-9
: https://doi.org/10.2478/s11756-010-0104-9

50. Expression and functional characterization of a Rho-family small GTPase CDC42 from Trichinella spiralis

Author:: Yang, Yurong; Qin, Weiwen; Tian, Guimei; Jian, Wei
Source:: Parasitology research 2010 v.107 no.1 pp. 153-162
ISSN:: 0932-0113
Subject:: Escherichia coli; RNA interference; Trichinella spiralis; Western blotting; adults; complementary DNA; early development; genes; guanosinetriphosphatase; muscle larvae; parasites; polyclonal antibodies; progeny
Abstract:: ... A full-length cDNA encoding a Rho-family small GTPase gene cdc42 of Trichinella spiralis was expressed in E. coli. The recombinant protein of TsCDC42 was purified and used to raise the polyclonal antibodies. The expression of TsCDC42 in different stages of parasite was investigated. The western blot showed that TsCDC42 was expressed in all stages of T. spiralis, including newborn larvae, muscle la ...
DOI:: 10.1007/s00436-010-1851-9
PubMed:: 20369253
: https://doi.org/10.1007/s00436-010-1851-9

51. Expression of Frizzled 2 in the mouse ovary during oestrous cycle

Author:: Wang, S.B.; Xing, B.S.; Yi, L.; Wang, W.; Xu, Y.X.
Source:: Journal of animal physiology and animal nutrition 2010 v.94 no.4 pp. 437-445
ISSN:: 0931-2439
Subject:: Western blotting; adults; cell differentiation; cell proliferation; corpus luteum; diestrus; estrus; gene expression; granulosa cells; immunohistochemistry; in situ hybridization; luteinization; messenger RNA; mice; oocytes; proestrus; proteins; signal transduction
Abstract:: ... The Wnt/β-catenin pathway is a conserved signalling pathway that regulates gene expression and controls diverse developmental processes such as cell fate specification, cell proliferation and cell differentiation. To our knowledge, the potential role of this pathway in the adult ovary has been poorly addressed. To this end, we investigated the expression pattern of Frizzled 2 in the mouse ovary du ...
DOI:: 10.1111/j.1439-0396.2009.00927.x
PubMed:: 19663975
: https://doi.org/10.1111/j.1439-0396.2009.00927.x

52. Generation of recombinant nucleocapsid protein of human metapneumovirus in baculovirus for detecting antibodies in the Beijing population

Author:: Liu, Liying; Qian, Yuan; Zhu, Runan; Zhao, Linqing; Deng, Jie
Source:: Archives of virology 2010 v.155 no.1 pp. 47-54
ISSN:: 0304-8608
Subject:: Human metapneumovirus; Western blotting; adults; antibodies; antibody detection; antigens; children; fluorescent antibody technique; insects; nucleocapsid; people; protein synthesis; recombinant proteins; seroprevalence; viruses; China
Abstract:: ... Human metapneumovirus (hMPV) is associated with respiratory infections in both adults and children. Recombinant nucleocapsid (N) proteins of hMPV from two major groups of hMPV in Beijing with a 6× His-tag at the C terminus were constructed in a baculovirus and expressed in transfected Sf21 insect cells. The antigenicity and specificity of the expressed recombinant proteins were examined by immunof ...
DOI:: 10.1007/s00705-009-0551-1
PubMed:: 19937067
: https://doi.org/10.1007/s00705-009-0551-1

53. Germ cell dynamics in the testis of the postnatal common marmoset monkey (Callithrix jacchus)

Author:: Albert, S.; Ehmcke, J.; Wistuba, J.; Eildermann, K.; Behr, R.; Schlatt, S.; Gromoll, J.
Source:: Reproduction 2010 v.140 no.5 pp. 733-742
ISSN:: 1470-1626
Subject:: Callithrix jacchus; Western blotting; adults; cell physiology; genetic markers; germ cells; humans; immunohistochemistry; messenger RNA; models; monkeys; morphometry; neonates; quantitative analysis; reverse transcriptase polymerase chain reaction; seminiferous epithelium; spermatogenesis; stem cells; testicular development
Abstract:: ... The seminiferous epithelium in the nonhuman primate Callithrix jacchus is similarly organized to man. This monkey has therefore been used as a preclinical model for spermatogenesis and testicular stem cell physiology. However, little is known about the developmental dynamics of germ cells in the postnatal primate testis. In this study, we analyzed testes of newborn, 8-week-old, and adult marmosets ...
DOI:: 10.1530/REP-10-0235
PubMed:: 20729334
: https://doi.org/10.1530/REP-10-0235

54. Identification of Dictyothrips betae as the vector of Polygonum ring spot virus

Author:: Ciuffo, M.; Mautino, G.C.; Bosco, L.; Turina, M.; Tavella, L.
Source:: Annals of applied biology 2010 v.157 no.2 pp. 299-307
ISSN:: 0003-4746
Subject:: Fallopia convolvulus; Frankliniella occidentalis; Polygonum; Thrips tabaci; Western blotting; adults; cytochrome-c oxidase; insects; larvae; leaves; phylogeny; reverse transcriptase polymerase chain reaction; ribosomal DNA; viruses
Abstract:: ... Dictyothrips betae (Thysanoptera: Thripidae) is the predominant thrips species on Polygonum convolvulus and Polygonum dumetorum plants infected with a recently described tospovirus species, Polygonum ring spot virus (PolRSV). Laboratory transmission experiments (leaf disk assays) with adults collected directly in the field demonstrated the competence of this thrips to transmit PolRSV, although onl ...
DOI:: 10.1111/j.1744-7348.2010.00428.x
: https://doi.org/10.1111/j.1744-7348.2010.00428.x

55. Immunoproteomic analysis of whole proteins from male and female adult Haemonchus contortus

Author:: Yan, Fengbin; Xu, Lixin; Liu, Liheng; Yan, Ruofeng; Song, Xiaokai; Li, Xiangrui
Source:: Veterinary journal 2010 v.185 no.2 pp. 174-179
ISSN:: 1090-0233
Subject:: Western blotting; sheep diseases; gastrointestinal nematodes; mass spectrometry; adults; proteome; nematode infections; males; actin; females; antigens; bioassays; immunization; vaccine development; proteomics; immunogenetics; gender differences; immunoglobulins; Haemonchus contortus; digestive system diseases; goat diseases
Abstract:: ... Whole proteins of male and female adult Haemonchus contortus were analysed by immunoproteomic techniques. Approximately 662 and 680 spots were detected on proteome maps of male and female nematodes, respectively, stained with Coomassie brilliant blue G-250. There were 609 shared spots. Approximately 193 and 196 spots were recognised on Western blot maps of male and female nematodes, respectively, ...
DOI:: 10.1016/j.tvjl.2009.05.021
PubMed:: 19560953
: https://doi.org/10.1016/j.tvjl.2009.05.021

56. Lung defects in neonatal and adult stromal-derived factor-1 conditional knockout mice

Author:: Chen, Wen-Cheng; Tzeng, Yi-Shiuan; Li, Hung; Tien, Wei-Sheng; Tsai, Ying-Chieh
Source:: Cell and tissue research 2010 v.342 no.1 pp. 75-85
ISSN:: 0302-766X
Subject:: Western blotting; adults; arginine; chemoattractants; fetal development; fluorescence; hematopoiesis; lungs; mice; neonates; nitric oxide; nitric oxide synthase; organogenesis; perinatal period; phosphorylation; tissue repair; ultrastructure
Abstract:: ... Stromal-derived factor (SDF)-1/CXCL12 is a cytokine that is involved in organogenesis, hematopoiesis, chemoattraction, and wound healing. An SDF-1 knockout mouse (SDF-1⁻/⁻) has provided important insights into the role of SDF-1 in fetal development. Because the SDF-1 knockout is lethal in the perinatal period, we have created a conditional SDF-1 knockout mouse. In the present study, we induced con ...
DOI:: 10.1007/s00441-010-1035-z
PubMed:: 20824284
: https://doi.org/10.1007/s00441-010-1035-z

57. Molecular and functional characterization of a mortalin-like protein from Schistosoma japonicum (SjMLP/hsp70) as a member of the HSP70 family

Author:: He, Sijie; Yang, Linlin; Lv, Zhiyue; Hu, Wei; Cao, Jianping; Wei, Jie; Sun, Xi; Yang, Jie; Zheng, Huanqin; Wu, Zhongdao
Source:: Parasitology research 2010 v.107 no.4 pp. 955-966
ISSN:: 0932-0113
Subject:: Escherichia coli; Schistosoma japonicum; Western blotting; adults; amino acids; bioinformatics; cDNA libraries; cercariae; complementary DNA; eggs; enzyme-linked immunosorbent assay; heat; heat shock proteins; heat tolerance; humans; immune response; interferon-gamma; intestines; mass spectrometry; mice; molecular weight; open reading frames; parasites; pathogenesis; recombinant vaccines; reverse transcriptase polymerase chain reaction; schistosomiasis; schistosomula; secretion; splenocytes
Abstract:: ... Schistosomes are the causative agent of schistosomiasis. The 70-kDa heat-shock proteins (HSP70) are considered the predominant HSP family and play a key regulatory role in parasite development and pathogenesis. Based on the published sequences in Genbank/EMBL, an open-reading frame (ORF) encoding 653 amino acids (XP_002581385.1) and belonging to the Schistosoma HSP70 protein family with a molecula ...
DOI:: 10.1007/s00436-010-1960-5
PubMed:: 20602114
: https://doi.org/10.1007/s00436-010-1960-5

58. Molecular cloning and characterization of a gene encoding methionine aminopeptidase 2 of Schistosoma japonicum

Author:: Peng, Jinbiao; Han, Hongxiao; Hong, Yang; Fu, Zhiqiang; Liu, Jinming; Lin, Jiaojiao
Source:: Parasitology research 2010 v.107 no.4 pp. 939-946
ISSN:: 0932-0113
Subject:: Microtus; Schistosoma japonicum; Western blotting; adults; gene expression; gene expression regulation; genes; hosts; methionine; mice; molecular cloning; praziquantel; rats; reverse transcriptase polymerase chain reaction; schistosomiasis; vaccination
Abstract:: ... Methionine aminopeptidase 2 (MAP2) is an essential enzyme that is involved in protein maturation. It plays a key role in the removal of the initiating methionine residue from nascent polypeptide chains. In the present study, a gene encoding methionine aminopeptidase 2 of Schistosoma japonicum (SjMAP2) was cloned and characterized. Real-time RT-PCR and Western blot analysis revealed that this was e ...
DOI:: 10.1007/s00436-010-1956-1
PubMed:: 20582436
: https://doi.org/10.1007/s00436-010-1956-1

59. Molecular cloning and characterization of cystatin, a cysteine protease inhibitor, from Angiostrongylus cantonensis

Author:: Liu, Yu-hong; Han, Yan-ping; Li, Zheng-yu; Wei, Jie; He, Han-jiang; Xu, Chang-zhi; Zheng, Huan-qin; Zhan, Xi-mei; Wu, Zhong-dao; Lv, Zhi-yue
Source:: Parasitology research 2010 v.107 no.4 pp. 915-922
ISSN:: 0932-0113
Subject:: Escherichia coli; Parastrongylus cantonensis; Western blotting; adults; cDNA libraries; cathepsin B; complementary DNA; cystatins; interferon-gamma; larvae; macrophages; mice; molecular cloning; nitric oxide; parasites; rats; reverse transcriptase polymerase chain reaction
Abstract:: ... Cystatins are thiol proteinase inhibitors ubiquitously present in mammalian body and serve various important physiological functions. In the present study, a novel cystatin molecule (AcCystatin) was cloned from a cDNA library of Angiostrongylus cantonensis fourth-stage larvae. The putative 14-kDa protein contained 120 residues with cystatin-conserved motifs known to interact with the active site o ...
DOI:: 10.1007/s00436-010-1952-5
PubMed:: 20567985
: https://doi.org/10.1007/s00436-010-1952-5

60. Molecular cloning and functional characterization of Schistosoma japonicum enolase which is highly expressed at the schistosomulum stage

Author:: Yang, Jianmei; Qiu, Chunhui; Xia, Yanxun; Yao, Lixiao; Fu, Zhiqiang; Yuan, Chunxiu; Feng, Xingang; Lin, Jiaojiao
Source:: Parasitology research 2010 v.107 no.3 pp. 667-677
ISSN:: 0932-0113
Subject:: Schistosoma japonicum; Western blotting; adults; antigens; eggs; enzyme activity; enzyme-linked immunosorbent assay; fecal egg count; fluorescent antibody technique; genes; glycolysis; humans; immunoglobulin G; liver; mice; molecular cloning; parasites; plasminogen; quantitative polymerase chain reaction; rabbits
Abstract:: ... Enolase is a key enzyme in the glycolytic pathway; recent studies have also shown that enolase is found on the surface of several parasites, where it acts as a plasminogen-binding protein. In the present study, the enolase of Schistosoma japonicum has been cloned and expressed. In western blot analysis, the recombinant enolase from S. japonicum ( rSjENO) was recognized by rabbit sera directed agai ...
DOI:: 10.1007/s00436-010-1913-z
PubMed:: 20512506
: https://doi.org/10.1007/s00436-010-1913-z

61. NAMPT (visfatin) in the chicken testis: influence of sexual maturation on cellular localization, plasma levels and gene and protein expression

Author:: Ocón-Grove, Olga M.; Krzysik-Walker, Susan M.; Maddineni, Sreenivasa R.; Hendricks, Gilbert L. III.; Ramachandran, Ramesh
Source:: Reproduction 2010 v.139 no.1 pp. 217-226
ISSN:: 1470-1626
Subject:: Leydig cells; NAD (coenzyme); Sertoli cells; Western blotting; adults; chickens; complementary DNA; cytoplasm; enzyme immunoassays; genes; immunohistochemistry; messenger RNA; nicotinamide phosphoribosyltransferase; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; seminal plasma; spermatids; spermatocytes; spermatogenesis; spermatozoa; steroidogenesis
Abstract:: ... Nicotinamide phosphoribosyltransferase (NAMPT) is a cytokine hormone and rate-limiting enzyme involved in production of NAD and therefore affects a variety of cellular functions requiring NAD. Spermatogenesis and testicular steroidogenesis are likely to depend on NAD-dependent reactions and may therefore be affected by changes in testicular NAMPT expression. The objectives of the present study are ...
DOI:: 10.1530/REP-08-0377
PubMed:: 19736255
: https://doi.org/10.1530/REP-08-0377

62. Red wine consumption improves in vitro migration of endothelial progenitor cells in young, healthy individuals

Author:: Hamed, Saher; Alshiek, Jonia; Aharon, Anat; Brenner, Benjamin; Roguin, Ariel
Source:: American journal of clinical nutrition 2010 v.92 no.1 pp. 161-169
ISSN:: 0002-9165
Subject:: signal transduction; Western blotting; receptors; cell differentiation; chemokines; adults; cardioprotective effect; gene expression; gene expression regulation; apoptosis; vascular tissues; in vitro studies; cell proliferation; red wines; food intake; endothelium; protein synthesis; endothelial cells; nitric oxide; biochemical pathways
Abstract:: ... BACKGROUND: Endothelial progenitor cells (EPCs) contribute to the maintenance of vascular endothelial function. The moderate consumption of red wine provides cardiovascular protection. OBJECTIVE: We investigated the underlying molecular mechanism of EPC migration in young, healthy individuals who drank red wine. DESIGN: Fourteen healthy volunteers consumed 250 mL red wine daily for 21 consecutive ...
DOI:: 10.3945/ajcn.2009.28408
PubMed:: 20392892
: https://doi.org/10.3945/ajcn.2009.28408

63. Schistosoma mansoni host-exposed surface antigens characterized by sera and recombinant antibodies from schistosomiasis-resistant rats

Author:: Sepulveda, Jorge; Tremblay, Jacqueline M.; DeGnore, Jon P.; Skelly, Patrick J.; Shoemaker, Charles B.
Source:: International journal for parasitology 2010 v.40 no.12 pp. 1407-1417
ISSN:: 0020-7519
Subject:: DNA libraries; Schistosoma mansoni; Western blotting; adults; epitopes; hosts; mice; proteomics; rats; recombinant antibodies; schistosomula; surface antigens
Abstract:: ... Antibodies from Schistosoma mansoni-infected rats, unlike mice, show a higher titer for schistosome apical tegumental antigens compared with non-apical membrane antigens. These antibodies bind to the surface of living lung-stage worms and to formaldehyde-fixed adult worms. We produced a single-chain antibody Fv domain (scFv) phage library displaying the antibody repertoire of rats highly immune to ...
DOI:: 10.1016/j.ijpara.2010.04.019
PubMed:: 20600071
PubMed Central:: PMC2939210
: https://doi.org/10.1016/j.ijpara.2010.04.019

64. Selective targeting of a TNFR decoy receptor pharmaceutical to the primate brain as a receptor-specific IgG fusion protein

Author:: Boado, Ruben J.; Hui, Eric Ka-Wai; Lu, Jeff Zhiqiang; Zhou, Qing-Hui; Pardridge, William M.
Source:: Journal of biotechnology 2010 v.146 no.1-2 pp. 84-91
ISSN:: 0168-1656
Subject:: Macaca mulatta; Western blotting; adults; biotechnology; blood-brain barrier; brain; chimerism; drugs; electrophoresis; gel chromatography; humans; immunoglobulin G; insulin receptors; monoclonal antibodies; radiolabeling; tumor necrosis factor-alpha
Abstract:: ... Decoy receptors, such as the human tumor necrosis factor receptor (TNFR), are potential new therapies for brain disorders. However, decoy receptors are large molecule drugs that are not transported across the blood-brain barrier (BBB). To enable BBB transport of a TNFR decoy receptor, the human TNFR-II extracellular domain was re-engineered as a fusion protein with a chimeric monoclonal antibody ( ...
DOI:: 10.1016/j.jbiotec.2010.01.011
PubMed:: 20100527
PubMed Central:: PMC2832096
: https://doi.org/10.1016/j.jbiotec.2010.01.011

65. Stage and tissue specific differences in SjBMI1, a Polycomb protein in Schistosoma japonicum

Author:: Hu, Dong; Wu, Jing; Hu, Fengyu; Yang, Yabo; Liang, Chi; Chen, Jingfang; Wang, Lexun; Wang, Peng; Wang, Xiaoyun; Xu, Jin; Hu, Xuchu; Yu, Xinbing
Source:: Parasitology research 2010 v.106 no.3 pp. 677-682
ISSN:: 0932-0113
Subject:: Schistosoma japonicum; Schistosoma mansoni; Western blotting; adults; amino acid sequences; animal ovaries; cercariae; eggs; genes; humans; reverse transcriptase polymerase chain reaction; stem cells; testes
Abstract:: ... Polycomb group protein BMI1, plays a central role in the stem cell pluripotency and development in metazoans. A gene encoding BMI1 homologue in the Schistosoma japonicum (SjBMI1) was cloned and identified. The deduced amino acid sequence shows high identity to the homologues from Schistosoma mansoni and Homo sapiens. Quantitative real time polymerase chain reaction (RT-PCR) and Western blot analys ...
DOI:: 10.1007/s00436-010-1734-0
PubMed:: 20098996
: https://doi.org/10.1007/s00436-010-1734-0

66. Toxic effects of lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on development of male reproductive system: Involvement of antioxidants, oxidants, and p53 protein

Author:: Jin, Mei Hua; Hong, Chang Hee; Lee, Hye Young; Kang, Hyo Jin; Han, Sang Won
Source:: Environmental toxicology 2010 v.25 no.1 pp. 1-8
ISSN:: 1520-4081
Subject:: Western blotting; milk; body length; oxidative stress; adults; mothers; hydrogen peroxide; spermatozoa; testes; tetrachlorodibenzo-p-dioxin; body weight; superoxide dismutase; mice; oxidants; progeny; testosterone; toxicity; antioxidant activity; adverse effects; lactation
Abstract:: ... 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a potent endocrine disruptor compound and induces multiple organ dysfunctions. The effect of TCDD exposure both in adults and in utero has been well established. However, little is known about the effects of TCDD acquired through mother's milk on the development of the male reproductive system. The aim of this study was to investigate the effects and m ...
DOI:: 10.1002/tox.20466
PubMed:: 19085997
: https://doi.org/10.1002/tox.20466

67. Transcriptional factor Pitx2: Localization during triton retina regeneration

Author:: Avdonin, P. P.; Grigoryan, E. N.; Markitantova, Yu. V.
Source:: Biology bulletin of the Russian Academy of Sciences 2010 v.37 no.3 pp. 231-235
ISSN:: 1062-3590
Subject:: Western blotting; adults; epithelium; hybridization; retina; transcription factors
Abstract:: ... For the first time immune-chemical analysis of transcriptional factor Pitx2 localization during triton retina regeneration after its removal and also in tissues of a nonoperated eye of an adult triton has been carried out. Protein Pitx2 has been found in the nucleus of the earliest neuroblasts that form the germ of the retina. At a later stage of retina regeneration, Pitx2 was found in the nucleus ...
DOI:: 10.1134/S1062359010030039
: https://doi.org/10.1134/S1062359010030039

68. Activation of calcium-sensing receptor increases TRPC3 expression in rat cardiomyocytes

Author:: Shan-Li Feng; Ming-Rui Sun; Ting-Ting Li; Xin Yin; Chang-Qing Xu; Yi-Hua Sun
Source:: Biochemical and biophysical research communications 2011 v.406 no.2 pp. 278-284
ISSN:: 0006-291X
Subject:: messenger RNA; confocal microscopy; sarcoplasmic reticulum; calcium; spermine; reverse transcriptase polymerase chain reaction; cardiomyocytes; Western blotting; adults; rats; receptors; gadolinium; apoptosis
Abstract:: ... Transient receptor potential (TRP) channels are expressed in cardiomyocytes, which gate a type of influx of extracellular calcium, the capacitative calcium entry. TRP channels play a role in mediating Ca²⁺ overload in the heart. Calcium-sensing receptors (CaR) are also expressed in rat cardiac tissue and promote the apoptosis of cardiomyocytes by Ca²⁺ overload. However, data about the link between ...
DOI:: 10.1016/j.bbrc.2011.02.033
: https://doi.org/10.1016/j.bbrc.2011.02.033

69. Characterization of cathepsin B proteinase (AcCP-2) in eggs and larvae stages of hookworm Ancylostoma caninum

Author:: Yurong Yang; Weiwen Qin; Hua Wei; Jianxi Ying; Jing Zhen
Source:: Experimental parasitology 2011 v.129 no.3 pp. 215-220
ISSN:: 0014-4894
Subject:: Ancylostoma caninum; Escherichia coli; Western blotting; adults; cathepsin B; complementary DNA; digestion; early development; eggs; enzyme activity; gelatin; hookworms; larvae; parasitology
Abstract:: ... Cathepsin B proteinase constitutes a large multigenes family in parasitic and non-parasitic nematodes. The localization of cathepsin B proteinases (AcCP-1 and AcCP-2) in adult worm of Ancylostoma caninum has been characterized (Harrop et al., 1995), but the localization and function in eggs and larval stages remained undiscovered. Here we described the expressing of cathepsin B proteinase (AcCP-2) ...
DOI:: 10.1016/j.exppara.2011.08.018
: https://doi.org/10.1016/j.exppara.2011.08.018

70. Cloning and expression analysis of a G‐protein subunit— in the rice water weevil lissorhoptrus oryzophilus Kuschel

Author:: Kang, Gui‐Juan; Gong, Zhong‐Jun; Cheng, Jia‐An; Mao, Cun‐Gui; Zhu, Zeng‐Rong
Source:: Archives of insect biochemistry and physiology 2011 v.76 no.1 pp. 43-54
ISSN:: 0739-4462
Subject:: G-proteins; Lissorhoptrus oryzophilus; Western blotting; adults; amino acids; antennae; dendrites; messenger RNA; molecular weight; open reading frames; parthenogenesis; quantitative polymerase chain reaction; sensilla; signal transduction
Abstract:: ... The open reading frame (ORF) encoding a novel G protein α subunit, Lo Gαo, was cloned from the parthenogenetic rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae). The Lo Gαo ORF encodes a protein of 354 amino acid residues. The deduced protein sequence shares high homology with Gαo from other species. The expression patterns of Lo Gαo in various adult tissues were ind ...
DOI:: 10.1002/arch.20403
: https://doi.org/10.1002/arch.20403

71. Cloning and expression of 21.1-kDa tegumental protein of Clonorchis sinensis and human antibody response to it as a trematode-nematode pan-specific serodiagnosis antigen

Author:: Chen, Jiajia; Xu, Hongxu; Zhang, Zhaoping; Zeng, Suxiang; Gan, Wenjia; Yu, Xinbing; Hu, Xuchu
Source:: Parasitology research 2011 v.108 no.1 pp. 161-168
ISSN:: 0932-0113
Subject:: Clonorchis sinensis; Escherichia coli; Fasciola hepatica; Nematoda; Schistosoma japonicum; Toxoplasma gondii; Western blotting; adults; affinity chromatography; antigens; bioinformatics; complementary DNA; cross reaction; enzyme-linked immunosorbent assay; humans; immunoglobulin G; immunohistochemistry; nematode infections; patients; plasmids; serodiagnosis
Abstract:: ... A complete cDNA encoding a 21.1-kDa tegumental protein (CsTP21.1) was recognized from Clonorchis sinensis adult full-length cDNA plasmid library by bioinformatics analysis. Recombinant CsTP21.1 was highly expressed in Escherichia coli, purified by affinity chromatography, and identified by Western blotting. Immunohistochemistry demonstrated that CsTP21.1 is localized in the tegument of the adult w ...
DOI:: 10.1007/s00436-010-2050-4
PubMed:: 20865432
: https://doi.org/10.1007/s00436-010-2050-4

72. Clonorchis sinensis enolase: Identification and biochemical characterization of a glycolytic enzyme from excretory/secretory products

Author:: Xiaoyun Wang; Wenjun Chen; Fengyu Hu; Chuanhuan Deng; Chenhui Zhou; Xiaoli Lv; Yongxiu Fan; Jingtao Men; Yan Huang; Jiufeng Sun; Dong Hu; Jingfang Chen; Yabo Yang; Chi Liang; Huanqin Zheng; Xuchu Hu; Jin Xu; Zhongdao Wu; Xinbing Yu
Source:: Molecular and biochemical parasitology 2011 v.177 no.2 pp. 135-142
ISSN:: 0166-6851
Subject:: Anopheles sinensis; Clonorchis sinensis; Escherichia coli; NAD (coenzyme); Western blotting; adults; blood serum; cDNA libraries; catalytic activity; cercariae; complementary DNA; eggs; energy metabolism; enzyme activity; excretory-secretory products; genes; glycolysis; immunohistochemistry; kynurenine pathway; monitoring; parasites; parasitology; transcription (genetics); vaccines
Abstract:: ... Enolase plays a key role in energy metabolism and development of most organisms. We isolated a gene encoding enolase from Clonorchis sinensis (C. sinensis) adult cDNA library and expressed the recombinant protein in Escherichia coli. C. sinensis enolase (Csenolase) was identified as both an excretory/secretory product and a tegumental component of C. sinensis by western blot analysis. The transcri ...
DOI:: 10.1016/j.molbiopara.2011.02.011
: https://doi.org/10.1016/j.molbiopara.2011.02.011

73. Deep bradycardia and heart block caused by inducible cardiac-specific knockout of the pacemaker channel gene Hcn4

Author:: Baruscotti, Mirko; Bucchi, Annalisa; Viscomi, Carlo; Mandelli, Giacomo; Consalez, Giacomo; Gnecchi-Rusconi, Tomaso; Montano, Nicola; Casali, Karina Rabello; Micheloni, Stefano; Barbuti, Andrea; DiFrancesco, Dario
Source:: Proceedings of the National Academy of Sciences of the United States of America 2011 v.108 no.4 pp. 1705-1710
ISSN:: 0027-8424
Subject:: Western blotting; adults; animal models; cardiac arrest; death; fluorescent antibody technique; genes; heart; hydrogen cyanide; mice; myocytes
Abstract:: ... Cardiac pacemaking generation and modulation rely on the coordinated activity of several processes. Although a wealth of evidence indicates a relevant role of the If ("funny," or pacemaker) current, whose molecular constituents are the hyperpolarization-activated, cyclic nucleotide-gated (HCN) channels and particularly HCN4, work with mice where Hcn genes were knocked out, or functionally modified ...
DOI:: 10.1073/pnas.1010122108
PubMed:: 21220308
PubMed Central:: PMC3029742
: https://doi.org/10.1073/pnas.1010122108

74. Expression of Nephrin, Podocin, α-Actinin-4 and α3-Integrin in Canine Renal Glomeruli

Author:: R. Kobayashi; J. Kamiie; K. Yasuno; K. Ogihara; K. Shirota
Source:: Journal of comparative pathology 2011 v.145 no.2-3 pp. 220-225
ISSN:: 0021-9975
Subject:: Beagle; Western blotting; adults; dog diseases; dogs; fluorescence microscopy; gene expression; kidneys; pathogenesis; proteins; reverse transcriptase polymerase chain reaction
Abstract:: ... The biological features of podocytes that contribute to the pathogenesis of proteinuria have not been investigated in dogs. The aim of this study was to investigate the expression and localization of nephrin, podocin, α-actinin-4 and α3-integrin in canine renal glomeruli. Renal cortical tissue was collected from the kidneys of five normal adult beagles. Western blotting and immunofluorescence micr ...
DOI:: 10.1016/j.jcpa.2011.01.007
: https://doi.org/10.1016/j.jcpa.2011.01.007

75. Expression of cyclin E in postmitotic neurons during development and in the adult mouse brain

Author:: Yayoi Ikeda; Yuko Matsunaga; Masahito Takiguchi; Masa-Aki Ikeda
Source:: Gene expression patterns 2011 v.11 no.1-2 pp. 64-71
ISSN:: 1567-133X
Subject:: Western blotting; adulthood; adults; cell cycle; cell nucleus; cerebellum; cerebral cortex; cultured cells; cyclins; cytoplasm; embryogenesis; fluorescent antibody technique; gene expression; immunohistochemistry; in situ hybridization; messenger RNA; mice; neonates; neurons; postnatal development; proliferating cell nuclear antigen
Abstract:: ... Cyclin E, a member of the G1 cyclins, is essential for the G1/S transition of the cell cycle in cultured cells, but its roles in vivo are not fully defined. The present study characterized the spatiotemporal expression profile of cyclin E in two representative brain regions in the mouse, the cerebral and cerebellar cortices. Western blotting showed that the levels of cyclin E increased towards adu ...
DOI:: 10.1016/j.gep.2010.09.004
: https://doi.org/10.1016/j.gep.2010.09.004

76. Expression of the IgSF protein Kirre in the rat central nervous system

Author:: Lingwei Kong; Roy C.Y. Choi; Heidi Q. Xie; Xidao Wang; Guangdun Peng; Zhiqin Xie; Karl W.K. Tsim; Naihe Jing
Source:: Life sciences 2011 v.88 no.13-14 pp. 590-597
ISSN:: 0024-3205
Subject:: Drosophila; Northern blotting; Western blotting; adults; amino acid sequences; brain stem; cDNA libraries; cerebellum; cerebral cortex; complementary DNA; genes; hippocampus; immunoglobulins; in situ hybridization; messenger RNA; neurites; neurodevelopment; rats; screening; spinal cord
Abstract:: ... AIMS: Immunoglobulin superfamily (IgSF) proteins play a critical role in development of the nervous system. Here, a new member of IgSF gene family was cloned from rat brain, which was subsequently identified as rat homolog of Drosophila Kirre. This new molecule was named as rat Kirre (rKirre). We aimed to reveal the developmental expression of rKirre, both at mRNA and protein levels, in the centra ...
DOI:: 10.1016/j.lfs.2011.01.014
: https://doi.org/10.1016/j.lfs.2011.01.014

77. Function and immuno-localization of aquaporins in the Antarctic midge Belgica antarctica

Author:: Shu-Xia Yi; Joshua B. Benoit; Michael A. Elnitsky; Nancy Kaufmann; Jeffrey L. Brodsky; Mark L. Zeidel; David L. Denlinger; Richard E. Lee Jr.
Source:: Journal of insect physiology 2011 v.57 no.8 pp. 1096-1105
ISSN:: 0022-1910
Subject:: Chironomidae; Drosophila; Malpighian tubules; Western blotting; adults; aquaporins; cell membranes; cold tolerance; fat body; freezing; mercuric chloride; midges; midgut; rehydration; Antarctic region
Abstract:: ... Aquaporin (AQP) water channel proteins play key roles in water movement across cell membranes. Extending previous reports of cryoprotective functions in insects, this study examines roles of AQPs in response to dehydration, rehydration, and freezing, and their distribution in specific tissues of the Antarctic midge, Belgica antarctica (Diptera, Chironomidae). When AQPs were blocked using mercuric ...
DOI:: 10.1016/j.jinsphys.2011.02.006
PubMed:: 21315725
: https://doi.org/10.1016/j.jinsphys.2011.02.006

78. Glyceraldehyde-3-phosphate dehydrogenase is a reliable internal control in Western blot analysis of leukocyte subpopulations from children

Author:: Hong-Ren Yu; Ho-Chang Kuo; Hsin-Chun Huang; Li-Tung Huang; You-Lin Tain; Chih-Cheng Chen; Chi-Di Liang; Jiunn-Ming Sheen; I-Chun Lin; Chi-Chiang Wu; Chia-Yu Ou; Kuender D. Yang
Source:: Analytical biochemistry 2011 v.413 no.1 pp. 24-29
ISSN:: 0003-2697
Subject:: Western blotting; actin; adults; children; glyceraldehyde-3-phosphate dehydrogenase; immunity; messenger RNA; monocytes; neonates; population; tubulin
Abstract:: ... To study differences in the development of immunity, leukocytes from cord blood are often compared with those from adult peripheral blood. Western blot analysis is a common method for detecting proteins. In this study, we investigated the reliability of using different housekeeping proteins (β-actin, β-tubulin, and glyceraldehyde-3-phosphate dehydrogenase [GAPDH]) as internal controls for differen ...
DOI:: 10.1016/j.ab.2011.01.037
: https://doi.org/10.1016/j.ab.2011.01.037

79. Hepatic effects of a highly purified 2,2′,3,4,4′,5,5′-heptachlorbiphenyl (PCB 180) in male and female rats

Author:: Robert Roos; Patrik L. Andersson; Krister Halldin; Helen Hǻkansson; Emma Westerholm; Timo Hamers; Gerd Hamscher; Päivi Heikkinen; Merja Korkalainen; Heather A. Leslie; Marjo Niittynen; Satu Sankari; Hans-Joachim Schmitz; Leo T.M. van der Ven; Matti Viluksela; Dieter Schrenk
Source:: Toxicology 2011 v.284 no.1-3 pp. 42-53
ISSN:: 0300-483X
Subject:: Western blotting; adults; alanine transaminase; blood serum; cytochrome P-450; females; gene expression; humans; hypertrophy; liver; males; messenger RNA; metabolism; polychlorinated biphenyls; rats; retinoids; toxicity; toxicology
Abstract:: ... PCB 180 (2,2′,3,4,4′,5,5′-heptachlorobiphenyl) is a persistent and accumulating polychlorinated biphenyl abundantly present in food and the environment. In this study, we used highly purified PCB 180 (dioxinlike impurities: 2.7ng TEQWHO/g PCB 180) in a 28-day toxicity study in young adult Sprague-Dawley rats. Male and female rats were given total doses of 3, 10, 30, 100, 300, 1000 or 1700mg/kg b.w ...
DOI:: 10.1016/j.tox.2011.03.013
: https://doi.org/10.1016/j.tox.2011.03.013

80. Hypermethylation of estrogen receptor promoter region in adult testis of rats exposed neonatally to bisphenol A

Author:: Tanvi Doshi; Smita Salian Mehta; Vikas Dighe; Nafisa Balasinor; Geeta Vanage
Source:: Toxicology 2011 v.289 no.2-3 pp. 74-82
ISSN:: 0300-483X
Subject:: DNA; DNA methylation; Western blotting; adulthood; adults; adverse effects; bisphenol A; epigenetics; epoxides; estrogen receptors; gene expression; manufacturing; methyltransferases; plastics; promoter regions; rats; resins; reverse transcriptase polymerase chain reaction; sesame oil; spermatogenesis; testes; toxicology
Abstract:: ... BACKGROUND: Bisphenol A (BPA) is an estrogenic endocrine disruptor commonly used in manufacture of polycarbonate plastics and epoxy resins. Due to its ubiquitous presence in the environment, health concerns are increasing. Earlier studies from our group have shown that neonatal exposure of male rats to BPA affected spermatogenesis leading to impairment in fertility during adulthood. Further we als ...
DOI:: 10.1016/j.tox.2011.07.011
: https://doi.org/10.1016/j.tox.2011.07.011

81. Identification of Brugia malayi immunogens by an immunoproteomics approach

Author:: Sirichit Wongkamchai; Wararat Chiangjong; Supachok Sinchaikul; Shui-Tein Chen; Wej Choochote; Visith Thongboonkerd
Source:: Journal of proteomics 2011 v.74 no.9 pp. 1607-1613
ISSN:: 1874-3919
Subject:: Brugia malayi; Western blotting; adults; antigens; data collection; filariasis; immunoglobulin G; immunoglobulin M; lymphangitis; patients
Abstract:: ... Filariasis remains a health problem in tropical countries. Identification of immunogens from its causative organism would lead to development of a better diagnostic test, as well as vaccine discovery to effectively prevent this disease. We applied immunoproteomics to define potential immunogens of adult Brugia malayi that were recognized by IgM, IgG1 and IgG4 in sera of patients with four distinct ...
DOI:: 10.1016/j.jprot.2011.06.012
PubMed:: 21704748
: https://doi.org/10.1016/j.jprot.2011.06.012

82. Identification of immuno-reactive adult Angiostrongylus vasorum proteins using mass spectrometry

Author:: Ryan Jefferies; Eric R. Morgan; Susan Shaw; Kate Heesom
Source:: Molecular and biochemical parasitology 2011 v.180 no.1 pp. 56-61
ISSN:: 0166-6851
Subject:: Angiostrongylus vasorum; Western blotting; adults; antibodies; aspartic proteinases; biosecurity; carnivores; dogs; electrophoresis; enzyme-linked immunosorbent assay; mass spectrometry; parasites; parasitology; proteinase inhibitors; proteins; vaccines; Western European region
Abstract:: ... Angiostrongylus vasorum is an emerging parasite of dogs and related carnivores throughout western Europe and presents a biosecurity threat to many countries worldwide. Infections are difficult to diagnose due to a high variability of clinical signs and can be fatal if left untreated. Previous attempts to develop indirect ELISA as a diagnostic test for antibody presence in dogs have been limited by ...
DOI:: 10.1016/j.molbiopara.2011.07.009
: https://doi.org/10.1016/j.molbiopara.2011.07.009

83. Identification of porcine polycystic kidney disease 1 (PKD1) gene: Molecular cloning, expression profile, and implication in disease model

Author:: Jin He; Qingsong Wang; Jianhua Ye; Xiaoxiang Hu; Ning Li
Source:: Gene 2011 v.490 no.1-2 pp. 37-46
ISSN:: 0378-1119
Subject:: autosomal dominant polycystic kidney disease; disease models; Western blotting; polycystins; promoter regions; adults; polypeptides; immunohistochemistry; methylation; pathogenesis; molecular weight; introns; transcription factors; alternative splicing; genomic islands; complementary DNA; humans; mice; molecular cloning; kidneys; exons; swine; animal models; binding sites
Abstract:: ... The polycystic kidney disease 1 (PKD1) gene, which accounts for ~85% of human autosomal dominant polycystic kidney disease (ADPKD) cases, has been extensively studied in human and mouse. Much information about the pathogenesis of and treatments for ADPKD has been gained from the use of mouse models. However, because mouse models pose some limitations, further studies in other model systems are nee ...
DOI:: 10.1016/j.gene.2011.08.027
PubMed:: 21945688
: https://doi.org/10.1016/j.gene.2011.08.027

84. Impact of Dermatophagoides pteronyssinus mite body raw material on house dust mite allergy diagnosis in a Serbian population

Author:: BURAZER, L.; MILOVANOVIC, K.; MILOVANOVIC, M.; VUCKOVIC, O.; VELICKOVIC, T.C.; GAVROVIC-JANKULOVIC, M.
Source:: Medical and veterinary entomology 2011 v.25 no.1 pp. 77-83
ISSN:: 0269-283X
Subject:: Dermatophagoides pteronyssinus; Western blotting; adults; dust; dust mites; hypersensitivity; immunoglobulin E; immunotherapy; patients; raw materials; selection criteria; serodiagnosis; Australia; Europe; South America
Abstract:: ... House dust mite (HDM) allergy has different clinical and immunological patterns in different geographic regions. The impact of raw material of commercial Dermatophadoides pteronyssinus (Acari: Pyroglyphidae) mite bodies on the quality of allergen extracts for allergy diagnosis in the Serbian population has not been previously evaluated. House dust mite bodies obtained from manufacturers in Europe, ...
DOI:: 10.1111/j.1365-2915.2010.00906.x
PubMed:: 20819152
: https://doi.org/10.1111/j.1365-2915.2010.00906.x

85. In vitro effects of gonadotropin-releasing hormone (GnRH) on Leydig cells of adult alpaca (Lama pacos) testis: GnRH receptor immunolocalization, testosterone and prostaglandin synthesis, and cyclooxygenase activities

Author:: M. Zerani; G. Catone; L. Quassinti; E. Maccari; M. Bramucci; A. Gobbetti; M. Maranesi; C. Boiti; F. Parillo
Source:: Domestic animal endocrinology 2011 v.40 no.1 pp. 51-59
ISSN:: 0739-7240
Subject:: Leydig cells; Sertoli cells; Western blotting; adults; alpacas; antagonists; aspirin; cytoplasm; enzyme activity; gonadotropin-releasing hormone; in vitro studies; phospholipase C; prostaglandin synthase; prostaglandins; spermatids; spermatocytes; spermatogonia; testosterone
Abstract:: ... The main objective of this study was to examine the modulatory in vitro effects of gonadotropin-releasing hormone (GnRH) on isolated Leydig cells of adult alpaca (Lama pacos) testis. We first evaluated the presence of GnRH receptor (GnRHR) and cyclooxygenase (COX) 1 and COX2 in alpaca testis. We then studied the in vitro effects of buserelin (GnRH analogue), antide (GnRH antagonist), and buserelin ...
DOI:: 10.1016/j.domaniend.2010.08.006
PubMed:: 20961724
: https://doi.org/10.1016/j.domaniend.2010.08.006

86. Larval fat body cells die during the early pupal stage in the frame of metamorphosis remodelation in Bombyx mori

Author:: Yu Kaneko; Thippawan Yasanga; Misa Suzuki; Sho Sakurai
Source:: Journal of insect physiology 2011 v.57 no.12 pp. 1715-1722
ISSN:: 0022-1910
Subject:: Bombyx mori; DNA fragmentation; Diptera; Western blotting; adults; apoptosis; caspase-3; ecdysterone; fat body; in vitro culture; insects; juvenile hormones; larvae; pupation
Abstract:: ... In holometabolus insects, morphology of the larval fat body is remodeled during metamorphosis. In higher Diptera, remodeling of the fat body is achieved by cell death of larval fat body cells and differentiation of the adult fat body from primordial cells. However, little is known about remodeling of the fat body at pupal metamorphosis in Lepidoptera. In this study, we found that cell death of the ...
DOI:: 10.1016/j.jinsphys.2011.09.013
PubMed:: 21971017
: https://doi.org/10.1016/j.jinsphys.2011.09.013

87. Localization of Insulin-Like Growth Factor-I (IGF-I) and IGF-I Receptor (IGF-IR) in Equine Testes

Author:: Yoon, MJ; Berger, T.; Roser, JF
Source:: Reproduction in domestic animals 2011 v.46 no.2 pp. 221-228
ISSN:: 0936-6768
Subject:: Leydig cells; Sertoli cells; Western blotting; adults; animal age; immunohistochemistry; insulin-like growth factor I; polyclonal antibodies; spermatocytes; spermatogenesis; spermatogonia; spermatozoa; stallions
Abstract:: ... The insulin-like growth factor-I (IGF-I) is a key regulator of reproductive functions. IGF-I actions are primarily mediated by IGF-IR. The main objective of this research was to evaluate the presence of IGF-I and IGF-I Receptor (IGF-IR) in stallion testicular tissue. The hypotheses of this study were (i) IGF-I and IGF-IR are present in stallion testicular cells including Leydig, Sertoli, and devel ...
DOI:: 10.1111/j.1439-0531.2010.01643.x
PubMed:: 20546172
: https://doi.org/10.1111/j.1439-0531.2010.01643.x

88. Malaria vaccine candidate: Design of a multivalent subunit α-helical coiled coil poly-epitope

Author:: Sope Olugbile; Viviane Villard; Sylvie Bertholet; Ali Jafarshad; Caroline Kulangara; Christian Roussilhon; Geraldine Frank; George W. Agak; Ingrid Felger; Issa Nebie; Karidia Konate; Andrey V. Kajava; Peter Schuck; Pierre Druilhe; François Spertini; Giampietro Corradin
Source:: Vaccine 2011 v.29 no.40 pp. 7090-7099
ISSN:: 0264-410X
Subject:: Western blotting; adults; antibodies; antigens; erythrocytes; fluorescent antibody technique; humans; malaria; mice; mononuclear leukocytes; parasites; peptides; proteins; vaccines; Senegal
Abstract:: ... A new strategy for the rapid identification of new malaria antigens based on protein structural motifs was previously described. We identified and evaluated the malaria vaccine potential of fragments of several malaria antigens containing α-helical coiled coil protein motifs. By taking advantage of the relatively short size of these structural fragments, we constructed different poly-epitopes in w ...
DOI:: 10.1016/j.vaccine.2011.06.122
PubMed:: 21803099
PubMed Central:: PMC4165486
: https://doi.org/10.1016/j.vaccine.2011.06.122

89. Molecular characterization of cathepsin B from Clonorchis sinensis excretory/secretory products and assessment of its potential for serodiagnosis of clonorchiasis

Author:: Wenjun Chen; Xiaoyun Wang; Xuerong Li; Xiaoli Lv; Chenhui Zhou; Chuanhuan Deng; Huali Lei; Jingtao Men; Yongxiu Fan; Chi Liang; Xinbing Yu
Source:: Parasites & vectors 2011 v.4 no.1 pp. 397
ISSN:: 1756-3305
Subject:: Clonorchis sinensis; Escherichia coli; Fasciola hepatica; Schistosoma japonicum; Western blotting; adults; amino acid sequences; cathepsin B; enzyme-linked immunosorbent assay; helminthiasis; humans; immunohistochemistry; parasites; pathogenesis; reverse transcriptase polymerase chain reaction; serodiagnosis; vaccines
Abstract:: ... BACKGROUND: Cathepsin cysteine proteases play multiple roles in the life cycle of parasites such as food uptake, immune invasion and pathogenesis, making them valuable targets for diagnostic assays, vaccines and drugs. The purpose of this study was to identify a cathepsin B of Clonorchis sinensis (CsCB) and to investigate its diagnostic value for human helminthiases. RESULTS: The predicted amino a ...
DOI:: 10.1186/1756-3305-4-149
PubMed:: 21794140
PubMed Central:: PMC3163202
: https://doi.org/10.1186/1756-3305-4-149

90. Molecular cloning, characterization, and immunolocalization of two lactate dehydrogenase homologous genes from Taenia solium

Author:: Wuying Du; Fengyu Hu; Yabo Yang; Dong Hu; Xuchu Hu; Xinbing Yu; Jin Xu; Jialin Dai; Xinjiang Liao; Jiang Huang
Source:: Parasitology research 2011 v.109 no.3 pp. 567-574
ISSN:: 0932-0113
Subject:: Danio rerio; Escherichia coli; Schistosoma japonicum; Sus scrofa; Taenia solium; Western blotting; Xenopus laevis; adults; amino acid sequences; antigens; cDNA libraries; complementary DNA; cross reaction; genes; humans; lactate dehydrogenase; molecular cloning; nucleotide sequences; physicochemical properties; rats; swine; tapeworms
Abstract:: ... Two novel genes encoding lactate dehydrogenase A (LDHA) and B (LDHB) homologues, respectively, were identified from the cDNA libraries of adult Taenia solium (T. solium). The two deduced amino acid sequences both show more than 50% identity to the homologues for Danio rerio, Xenopus laevis, Schistosoma japonicum, Sus scrofa, Homo sapiens, et al. The identity of the amino acid sequence between TsLD ...
DOI:: 10.1007/s00436-011-2285-8
PubMed:: 21331787
: https://doi.org/10.1007/s00436-011-2285-8

91. Molecular cloning, expression, and characterization of cyclophilin A from Clonorchis sinensis

Author:: Weihua Wu; Jiajia Chen; Suxiang Zeng; Zhaoping Zhang; Wenjia Gan; Xinbing Yu; Xuchu Hu
Source:: Parasitology research 2011 v.109 no.2 pp. 345-351
ISSN:: 0932-0113
Subject:: correlation; immunoglobulin G; liver; antigens; immunohistochemistry; secretion; inflammation; epithelium; Western blotting; enzyme-linked immunosorbent assay; genes; cDNA libraries; antiserum; molecular cloning; Clonorchis sinensis; patients; cyclophilins; signal peptide; adults; hyperplasia
Abstract:: ... This study described the recognization, cloning, and recombinant expression of cyclophilin A-like gene from Clonorchis sinensis adult complementary DNA library (CsCyPA) and its expression and secretion in adult. Western blotting demonstrated the recombinant CsCyPA could be recognized by sera of clonorchiasis patients and a sole protein of the same size in the excretory-secretory antigens of in vit ...
DOI:: 10.1007/s00436-011-2262-2
PubMed:: 21360097
: https://doi.org/10.1007/s00436-011-2262-2

92. Molecular identification and expression analysis of lipocalins from blood feeding taiga tick, Ixodes persulcatus Schulze

Author:: Satoru Konnai; Hideto Nishikado; Shinji Yamada; Saiki Imamura; Takuya Ito; Misao Onuma; Shiro Murata; Kazuhiko Ohashi
Source:: Experimental parasitology 2011 v.127 no.2 pp. 467-474
ISSN:: 0014-4894
Subject:: Ixodes persulcatus; Ixodes ricinus; Western blotting; adults; antibodies; blood; engorgement; expressed sequence tags; gene expression; gene expression regulation; immune response; mice; parasitology; reverse transcriptase polymerase chain reaction; saliva; salivary glands; taiga; ticks; vaccination
Abstract:: ... Lipocalins have been known for their several biological activities in blood-sucking arthropods. Recently, the identification and characterization of lipocalins from Ixodes ricinus (LIRs) have been reported and functions of lipocalins are well documented. In this study, we have characterized four Ixodes persulcatus lipocalins that were discovered while analyzing I. persulcatus tick salivary gland E ...
DOI:: 10.1016/j.exppara.2010.10.002
: https://doi.org/10.1016/j.exppara.2010.10.002

93. Molecular mechanism underlying the differential MYF6 expression in postnatal skeletal muscle of Duroc and Pietrain breeds

Author:: Huitao Fan; Mehmet Ulas Cinar; Chirawath Phatsara; Dawit Tesfaye; Ernst Tholen; Christian Looft; Karl Schellander
Source:: Gene 2011 v.486 no.1-2 pp. 8-14
ISSN:: 0378-1119
Subject:: DNA; DNA methylation; Duroc; Pietrain; Western blotting; acetylation; adults; breeding; epigenetics; genes; histones; loins (meat cut); lysine; messenger RNA; muscles; phenotype; pork; protein synthesis; reverse transcriptase polymerase chain reaction; skeletal muscle; swine; transcriptional activation
Abstract:: ... Among modern western pigs, Duroc (high meat fat ratio) and Pietrain (low meat fat ratio) breeds extensively utilized in commercial pork production differ extremely for their muscle phenotypes. The molecular mechanism, especially the epigenetic mechanism, underlying these breed-specific differences is poorly known. Myogenic factor 6 (MYF6) is the most abundantly expressed myogenic factor in adult m ...
DOI:: 10.1016/j.gene.2011.06.031
PubMed:: 21749918
: https://doi.org/10.1016/j.gene.2011.06.031

94. Morphological and functional alterations in adult boar epididymis: Effects of prenatal and postnatal administration of flutamide

Author:: Lydka, Marta; Kopera-Sobota, Ilona; Kotula-Balak, Malgorzata; Chojnacka, Katarzyna; Zak, Dorota; Bilinska, Barbara
Source:: Acta veterinaria scandinavica 2011 v.53 pp. 115-122
ISSN:: 1751-0147
Subject:: Western blotting; adults; apoptosis; boars; epididymis; histology; parturition; proteins; stromal cells
Abstract:: ... Background: The dynamic cross-talk between epididymal cells is hormonally regulated and, in part, through direct cell-to-cell interactions. To date, no information is available regarding possible impact of anti-androgens on the proteins involved in the gap junctional communication within the boar epididymis. Thus, a question arised whether prenatal or postnatal exposure to an anti-androgen flutami ...
DOI:: 10.1186/1751-0147-53-12
PubMed:: 21342526
PubMed Central:: PMC3050768
: https://doi.org/10.1186/1751-0147-53-12

95. Occurrence of a conserved domain in ATP diphosphohydrolases from pathogenic organisms associated to antigenicity in human parasitic diseases

Author:: Ana Carolina Ribeiro Gomes Maia; Michelle Lima Detoni; Gabriane Nascimento Porcino; Thais Vieira Soares; Michelia Antônia do Nascimento Gusmão; Melissa Regina Fessel; Marcos José Marques; Maria Aparecida Souza; Paulo Marcos Zech Coelho; Juliana Assis Silva Gomes Estanislau; Manoel Otávio da Costa Rocha; Marcelo de Oliveira Santos; Priscila Faria-Pinto; Eveline Gomes Vasconcelos
Source:: Developmental and comparative immunology 2011 v.35 no.10 pp. 1059-1067
ISSN:: 0145-305X
Subject:: Leishmania braziliensis; Schistosoma mansoni; Western blotting; adults; antibodies; apyrase; coevolution; enzyme-linked immunosorbent assay; humans; immune response; immunoglobulin G; leishmaniasis; mice; parasites; patients; polypeptides; potatoes; protozoan proteins; schistosomiasis
Abstract:: ... A polypeptide (r78–117) belonging to the potato apyrase was identified as a conserved domain shared with apyrase-like proteins from distinct pathogenic organisms, and was obtained as a 6xHis tag polypeptide (r-Domain B). By ELISA, high IgG, and IgG1 and IgG2a subtypes levels were detected in BALB/c mice pre-inoculated with r-Domain B. In Schistosoma mansoni adult worm or Leishmania (V.) braziliens ...
DOI:: 10.1016/j.dci.2011.03.026
: https://doi.org/10.1016/j.dci.2011.03.026

96. Ontogenesis of UDP-glucuronosyltransferase enzymes in sheep

Author:: Manoja Pretheeban; Geoff Hammond; Stelvio Bandiera; Wayne Riggs; Dan Rurak
Source:: Comparative biochemistry and physiology 2011 v.159 no.2 pp. 159-166
ISSN:: 1095-6433
Subject:: transcription factors; messenger RNA; metabolism; liver; complementary DNA; humans; drugs; antibodies; enzymes; ontogeny; rapid amplification of cDNA ends; Western blotting; neonates; fetus; lambs; nucleotide sequences; cortisol; adults
Abstract:: ... UDP glucuronosyltransferases (UGTs) are important for the metabolism of many drugs. To understand the ontogeny of these enzymes in sheep (Ovis aries), knowledge of their expression levels at different developmental stages is important. cDNA sequences of six UGTs (UGT1A1, UGT1A3, UGT1A4, UGT1A6 and UGT1A9) were cloned in sheep liver by rapid amplification of cDNA ends. The mRNA and protein levels o ...
DOI:: 10.1016/j.cbpa.2011.02.014
: https://doi.org/10.1016/j.cbpa.2011.02.014

97. Oxidative stress increases SNAT1 expression and stimulates cysteine uptake in freshly isolated rat cardiomyocytes

Author:: King, Nicola; Lin, Hua; Suleiman, M.-Saadeh
Source:: Amino acids 2011 v.40 no.2 pp. 517-526
ISSN:: 0939-4451
Subject:: Western blotting; adults; alanine; cardiomyocytes; cysteine; filtration; glutamine; glutathione; hydrogen peroxide; males; oils; oxidative stress; radionuclides; rats; reverse transcriptase polymerase chain reaction; serine; sulfur; transporters
Abstract:: ... Intracellular cysteine availability is an important rate-limiting factor governing glutathione synthesis in the heart. This is also dependent on the magnitude and rate of cysteine uptake into cardiomyocytes, which has been little studied. This study investigated the hypothesis that changes to cysteine transporter expression and activity during oxidative stress influence cardiomyocyte glutathione l ...
DOI:: 10.1007/s00726-010-0664-6
PubMed:: 20602128
PubMed Central:: PMC3044454
: https://doi.org/10.1007/s00726-010-0664-6

98. Pattern of expression of the CREG gene and CREG protein in the mouse embryo

Author:: Yang, Guitang; Han, Yaling; Tian, Xiaoxiang; Tao, Jie; Sun, Mingyu; Kang, Jian; Yan, Chenghui
Source:: Molecular biology reports 2011 v.38 no.3 pp. 2133-2140
ISSN:: 0301-4851
Subject:: Western blotting; actin; adults; blood cells; blood vessels; brain; cell proliferation; embryogenesis; endothelial cells; genes; glycoproteins; heart; immunohistochemistry; intestines; kidneys; liver; lungs; mice; myocytes; reverse transcriptase polymerase chain reaction; smooth muscle
Abstract:: ... The cellular repressor of E1A-stimulated genes (CREG) is a secreted glycoprotein that inhibits cell proliferation and/or enhances differentiation. CREG is widely expressed in adult tissues such as the brain, heart, lungs, liver, intestines and kidneys in mice. We investigated the level of CREG expression during mouse embryogenesis and its distribution at 18.5 days post coitus (dpc) using immunohis ...
DOI:: 10.1007/s11033-010-0340-7
: https://doi.org/10.1007/s11033-010-0340-7

99. Pituitary Adenylate Cyclase‐Activating Polypeptide‐Immunoreactive Cells in the Ageing Gerbil Hippocampus

Author:: P. Du; C. H. Lee; J. H. Choi; K. ‐Y. Yoo; Y. L. Lee; I. ‐J. Kang; I. K. Hwang; J. ‐D. Kim; M. ‐H. Won
Source:: Anatomia, histologia, embryologia 2011 v.40 no.5 pp. 389-396
ISSN:: 0340-2096
Subject:: Western blotting; adults; gerbils; hippocampus
Abstract:: ... With 4 figures and 2 tables SUMMARY: In the present study, we investigated age‐related changes in pituitary adenylate cyclase‐activating polypeptide (PACAP) immunoreactivity and its protein levels in the gerbil hippocampus at various ages using immunohistochemistry and western blot analysis. In the post‐natal month 1 (PM 1) group, PACAP‐immunoreactive cells were found in all hippocampal subregions ...
DOI:: 10.1111/j.1439-0264.2011.01083.x
PubMed:: 21545645
: https://doi.org/10.1111/j.1439-0264.2011.01083.x

100. Pre-treatment with isoflurane ameliorates renal ischemic–reperfusion injury in mice

Author:: Lei Zhang; Han Huang; Jian Cheng; Jin Liu; Hailin Zhao; Marcela P. Vizcaychipi; Daqing Ma
Source:: Life sciences 2011 v.88 no.25-26 pp. 1102-1107
ISSN:: 0024-3205
Subject:: Western blotting; adults; air; apoptosis; blood plasma; creatinine; erythropoietin; hydrodynamics; hypoxia; isoflurane; kidneys; mice; oxygen; pretreatment; protective effect; small interfering RNA; survival rate; tail; urea
Abstract:: ... AIMS: Perioperative renal dysfunction is associated with a high mortality. The aim of this study was to investigate whether isoflurane preconditioning provides a protection against renal ischemic–reperfusion injury and whether hypoxia inducible factor 1α (HIF-1α) is responsible for the protection afforded by isoflurane in mice. MAIN METHODS: Adult male C57BL/6 mice received vehicle (PBS), scramble ...
DOI:: 10.1016/j.lfs.2011.04.004
: https://doi.org/10.1016/j.lfs.2011.04.004

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1. Expression of Leˣ antigen in Schistosoma japonicum and S.haematobium and immune responses to Leˣ in infected animals: Lack of Leˣ expression in other trematodes and nematodes

2. Expression of the Lewis group carbohydrate antigens during Xenopus development

3. Ontogeny and subcellular localization of rat liver mitochondrial branched chain amino‐acid aminotransferase

4. UV‐A‐related alterations of young and adult lens water‐insoluble α‐crystallin, plasma membranous and cytoskeletal proteins

5. Cellular Distribution of Anionic Antimicrobial Peptide in Normal Lung and during Acute Pulmonary Inflammation

6. Acute effects of ethanol on feeding behavior and leptin-induced STAT3 phosphorylation in rat hypothalamus

7. Humoral and cellular response in lambs vaccinated against Haemonchus contortus with p26/23

8. Monoclonal anti-equine IgE antibodies with specificity for different epitopes on the immunoglobulin heavy chain of native IgE

9. Phase- and Age-Related Differences in Protein Tyrosine Phosphorylation in Sequoia sempervirens

10. Regulation of L-leucine transport in rat kidney by dexamethasone and triiodothyronine

11. Effect of daily administration of pyrantel tartrate in preventing infection in horses experimentally challenged with Sarcocystis neurona

12. Expression of Col1a1, Col1a2 and procollagen I in germ cells of immature and adult mouse testis

13. In vitro translation of RNA to lactase during postnatal development of rat intestine

14. Cloning and Characterization of a Novel, Human Cellular Retinaldehyde-binding Protein CRALBP-like (CRALBPL) Gene

15. Selective expression of the scaffold protein JSAP1 in spermatogonia and spermatocytes

16. Faithful tissue-specific expression of the human chromosome 21-linked COL6A1 gene in BAC-transgenic mice

17. Molecular characterization and serodiagnosis analysis of a novel lysophospholipase from Clonorchis sinensis

18. Preliminary characterization of an adult worm “vomit” preparation of Schistosoma mansoni and its potential use as antigen for diagnosis

19. Proline-rich Gla protein 2 is a cell-surface vitamin K-dependent protein that binds to the transcriptional coactivator Yes-associated protein

20. Thermally induced and developmentally regulated expression of a small heat shock protein in Trichinella spiralis

21. ArHsp22, a developmentally regulated small heat shock protein produced in diapause-destined Artemia embryos, is stress inducible in adults

22. CCDC134, a novel secretory protein, inhibits activation of ERK and JNK, but not p38 MAPK

23. Cloning and characterization of a cDNA fragment encoding a Schistosoma mansoni actin-binding protein (Smfilamin)

24. Essential Role of Chitinase in the Development of the Filarial Nematode Acanthocheilonema viteae

25. Foot-shock Stress-Induced Regional Iron Accumulation and Altered Iron Homeostatic Mechanisms in Rat Brain

26. In Vitro Induction of 60-kDa and 70-kDa Heat Shock Proteins by Endosulphan and Monocrotophos in Sheep Blowfly Lucilia cuprina

27. Molecular Characterization and Expression Pattern of Fetuin-B in Gibel Carp (Carassius auratus gibelio)

28. Recognition and characterization of TGF-β receptor interacting protein 1 (TRIP-1) containing WD40 repeats from Clonorchis sinensis by bioinformatics, cloning, and expression in Escherichia coli

29. The secretome of the filarial parasite, Brugia malayi: Proteomic profile of adult excretory-secretory products

30. Amphibian larvae and zinc sulphate: a suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium

31. Analysis of OCT4 expression in an extended panel of human tumor cell lines from multiple entities and in human mesenchymal stem cells

32. Angiogenic properties of adult human thymus fat

33. Characterization of phosphodiesterase-5 as a surface protein in the tegument of Schistosoma mansoni

34. Cloning, expression and functional analysis of a general odorant-binding protein 2 gene of the rice striped stem borer, Chilo suppressalis (Walker) (Lepidoptera: Pyralidae)

35. Cloning, molecular characterization of a 13-kDa antigen from Schistosoma japonicum, Sj13, a putative salivary diagnosis candidate for Schistosomiasis japonica

36. Differential localization of α' and β subunits of protein kinase CK2 during rat spermatogenesis

37. Expression and characterization of aspartic protease gene in eggs and larvae stage of Ancylostoma caninum

38. Expression profile, localization of an 8-kDa calcium-binding protein from Schistosoma japonicum (SjCa8), and vaccine potential of recombinant SjCa8 (rSjCa8) against infections in mice

39. Fat diet affects leptin receptor levels in the rat cerebellum

40. Glycotope analysis in miracidia and primary sporocysts of Schistosoma mansoni: Differential expression during the miracidium-to-sporocyst transformation

41. Identification and characterization of antimicrobial peptide, defensin, in the taiga tick, Ixodes persulcatus

42. Isolation and immunolocalization of a putative protective antigen (p26/23) from adult Haemonchus contortus

43. Molecular characterization of a novel Clonorchis sinensis secretory phospholipase A₂ and investigation of its potential contribution to hepatic fibrosis

44. Molecular cloning and analysis of stage and tissue-specific expression of Cathepsin L-like protease from Clonorchis sinensis

45. The functional expression and characterisation of a cysteine peptidase from the invasive stage of the neuropathogenic schistosome Trichobilharzia regenti

46. The ubiquitin-proteasome system in Strongyloididae. Biochemical evidence for developmentally regulated proteolysis in Strongyloides venezuelensis

47. Weak effect of trans-10, cis-12-conjugated linoleic acid on body fat accumulation in adult hamsters

48. Aquaporin AQP11 in the testis: molecular identity and association with the processing of residual cytoplasm of elongated spermatids

49. Effect of RH-2485 on development, metamorphosis and synthesis of major proteins in female silkworm Bombyx mori

50. Expression and functional characterization of a Rho-family small GTPase CDC42 from Trichinella spiralis

51. Expression of Frizzled 2 in the mouse ovary during oestrous cycle

52. Generation of recombinant nucleocapsid protein of human metapneumovirus in baculovirus for detecting antibodies in the Beijing population

53. Germ cell dynamics in the testis of the postnatal common marmoset monkey (Callithrix jacchus)

54. Identification of Dictyothrips betae as the vector of Polygonum ring spot virus

55. Immunoproteomic analysis of whole proteins from male and female adult Haemonchus contortus

56. Lung defects in neonatal and adult stromal-derived factor-1 conditional knockout mice

57. Molecular and functional characterization of a mortalin-like protein from Schistosoma japonicum (SjMLP/hsp70) as a member of the HSP70 family

58. Molecular cloning and characterization of a gene encoding methionine aminopeptidase 2 of Schistosoma japonicum

59. Molecular cloning and characterization of cystatin, a cysteine protease inhibitor, from Angiostrongylus cantonensis

60. Molecular cloning and functional characterization of Schistosoma japonicum enolase which is highly expressed at the schistosomulum stage

61. NAMPT (visfatin) in the chicken testis: influence of sexual maturation on cellular localization, plasma levels and gene and protein expression

62. Red wine consumption improves in vitro migration of endothelial progenitor cells in young, healthy individuals

63. Schistosoma mansoni host-exposed surface antigens characterized by sera and recombinant antibodies from schistosomiasis-resistant rats

64. Selective targeting of a TNFR decoy receptor pharmaceutical to the primate brain as a receptor-specific IgG fusion protein

65. Stage and tissue specific differences in SjBMI1, a Polycomb protein in Schistosoma japonicum

66. Toxic effects of lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on development of male reproductive system: Involvement of antioxidants, oxidants, and p53 protein

67. Transcriptional factor Pitx2: Localization during triton retina regeneration

68. Activation of calcium-sensing receptor increases TRPC3 expression in rat cardiomyocytes

69. Characterization of cathepsin B proteinase (AcCP-2) in eggs and larvae stages of hookworm Ancylostoma caninum

70. Cloning and expression analysis of a G‐protein subunit— in the rice water weevil lissorhoptrus oryzophilus Kuschel