Subject term: Western blotting and adults

1. 2,5-Hexanedione altered the degradation of low-molecular-weight neurofilament in rat nerve tissues

Author:: Fuyong Song; Qingguo Zhang; Ruirui Kou; Chaoshuang Zou; Yuan Gao; Keqin Xie
Source:: Food and chemical toxicology 2012 v.50 no.12 pp. 4277-4284
ISSN:: 0278-6915
Subject:: Western blotting; adults; autophagy; hexane; nerve tissue; occupational exposure; pathogenesis; peripheral nervous system diseases; rats; toxicology
Abstract:: ... Occupational exposure to n-hexane produces a central–peripheral distal axonopathy, which is characterized by giant axonal swellings filled with neurofilaments (NFs). To investigate the change of NFs degradation and their possible role in n-hexane neuropathy, adult male Wistar rats were administered intraperitoneally at a dosage of 400mg/kg/day 2,5-hexanedione (2,5-HD) for 4weeks. The time course o ...
DOI:: 10.1016/j.fct.2012.08.049
: https://doi.org/10.1016/j.fct.2012.08.049

2. 20(S)-Ginsenoside Rh2 Induce the Apoptosis and Autophagy in U937 and K562 Cells

Author:: Jianjian Zhuang; Juxin Yin; Chaojian Xu; Ying Mu; Shaowu Lv
Source:: Nutrients 2018 v.10 no.3 pp. -
ISSN:: 2072-6643
Subject:: Panax ginseng; Western blotting; adults; apoptosis; autophagy; bioactive compounds; cell proliferation; flow cytometry; fluorescence microscopy; myeloid leukemia; quantitative polymerase chain reaction; transmission electron microscopy
Abstract:: ... Acute myeloid leukemia (AML) and Chronic myelogenous leukemia (CML) are common leukemia in adults. 20(S)-GRh2 is an important bioactive substance that is present in Panax ginseng. However, there are no investigations that deal with the comparison of apoptosis, the occurrence of autophagy, and the relationship between apoptosis and autophagy after being treated with 20(S)-GRh2 in AML and CML. In th ...
DOI:: 10.3390/nu10030328
PubMed:: 29518056
PubMed Central:: PMC5872746
: https://doi.org/10.3390/nu10030328

3. 41.5-kDa Cathepsin L protease from Clonorchis sinensis: expression, characterization, and serological reactivity of one excretory–secretory antigen

Author:: Yanwen Li; Yan Huang; Xuchu Hu; Xiaoquan Liu; Changling Ma; Junhong Zhao; Zhongdao Wu; Jin Xu; Xinbing Yu
Source:: Parasitology research 2012 v.111 no.2 pp. 673-680
ISSN:: 0932-0113
Subject:: Clonorchis sinensis; Escherichia coli; Schistosoma japonicum; Western blotting; adults; amino acid sequences; amino acids; antigens; cDNA libraries; cathepsin L; complementary DNA; eggs; enzyme-linked immunosorbent assay; excystation; immune response; immunoglobulin G; inclusion bodies; intestines; pathogenicity; patients; serodiagnosis; tail; uterus
Abstract:: ... Cysteine proteases (CPs) were associated with the pathogenicity and excystment of Clonorchis sinensis. Most of them were potential antigens for the immunodiagnosis of clonorchiasis. More researches on CPs will let us know more about their functions, and further employ them for the development of more efficient diagnostic reagent and prevention strategies. In the current study, a full-length sequen ...
DOI:: 10.1007/s00436-012-2885-y
PubMed:: 22406988
: https://doi.org/10.1007/s00436-012-2885-y

4. A Serological Survey About Zoonoses in the Verkhoyansk Area, Northeastern Siberia (Sakha Republic, Russian Federation)

Author:: Magnaval Jean-François; Leparc-Goffart Isabelle; Gibert Morgane; Gurieva Alla; Outreville Jonathan; Dyachkovskaya Praskovia; Fabre Richard; Fedorova Sardana; Nikolaeva Dariya; Dubois Damien; Melnitchuk Olga; Daviaud-Fabre Pascale; Marty Marie; Alekseev Anatoly; Crubezy Eric
Source:: Vector borne and zoonotic diseases 2016 v.16 no.2 pp. 103-109
ISSN:: 1557-7759
Subject:: immunoglobulin M; Western blotting; cysticercosis; adults; immunoglobulin G; seroprevalence; enzyme-linked immunosorbent assay; tick-borne encephalitis; volunteers; trichinosis; echinococcosis; Q fever; toxoplasmosis; toxocariasis; serological surveys; Lyme disease; zoonoses; West Nile virus; villages; Arctic region; Siberia
Abstract:: ... In 2012, a seroprevalence survey concerning 10 zoonoses, which were bacterial (Lyme borreliosis and Q fever), parasitic (alveolar echinococcosis [AE] and cystic echinococcosis [CE], cysticercosis, toxoplasmosis, toxocariasis, and trichinellosis), or arboviral (tick-borne encephalitis and West Nile virus infection), was conducted among 77 adult volunteers inhabiting Suordakh and Tomtor Arctic villa ...
DOI:: 10.1089/vbz.2015.1828
: https://doi.org/10.1089/vbz.2015.1828

5. A Sertoli cell-specific connexin43 knockout leads to altered interstitial connexin expression and increased Leydig cell numbers

Author:: Joanna Noelke; Joachim Wistuba; Oliver S. Damm; Daniela Fietz; Jonathan Gerber; Marion Gaehle; Ralph Brehm
Source:: Cell and tissue research 2015 v.361 no.2 pp. 633-644
ISSN:: 0302-766X
Subject:: quantitative polymerase chain reaction; spermatogonia; messenger RNA; translation (genetics); Leydig cells; immunohistochemistry; connexins; steroidogenesis; tissue analysis; Western blotting; mice; testosterone; reverse transcription; in vitro studies; Sertoli cells; adults; hyperplasia
Abstract:: ... The Sertoli cell (SC)-specific knockout (KO) of connexin43 (Cx43) results in spermatogenic arrest at the level of spermatogonia and/or SC-only syndrome. Histology of the interstitial compartment suggests Leydig cell (LC) hyperplasia. Our aim has been to investigate possible effects of the SC-specific KO of Cx43 (SCCx43KO) on interstitial LC. We therefore counted LC via the optical dissector method ...
DOI:: 10.1007/s00441-015-2126-7
: https://doi.org/10.1007/s00441-015-2126-7

6. A glutathione S-transferase gene associated with antioxidant properties isolated from Apis cerana cerana

Author:: Shuchang Liu; Feng Liu; Haihong Jia; Yan Yan; Hongfang Wang; Xingqi Guo; Baohua Xu
Source:: Naturwissenschaften 2016 v.103 no.5-6 pp. 43
ISSN:: 0028-1042
Subject:: Apis cerana; Escherichia coli; Western blotting; abiotic stress; adults; amino acid sequences; antioxidant activity; antioxidants; gene expression; genes; glutathione; glutathione transferase; insecticide resistance; insecticides; insects; microbial growth; oxidative stress; phylogeny; quantitative polymerase chain reaction
Abstract:: ... Glutathione S-transferases (GSTs) are an important family of multifunctional enzymes in aerobic organisms. They play a crucial role in the detoxification of exogenous compounds, especially insecticides, and protection against oxidative stress. Most previous studies of GSTs in insects have largely focused on their role in insecticide resistance. Here, we isolated a theta class GST gene designated A ...
DOI:: 10.1007/s00114-016-1362-3
: https://doi.org/10.1007/s00114-016-1362-3

7. A kinetic characterization of the gill V(H+)-ATPase in juvenile and adult Macrobrachium amazonicum, a diadromous palaemonid shrimp

Author:: Malson N. Lucena; Marcelo R. Pinto; Daniela P. Garçon; John C. McNamara; Francisco A. Leone
Source:: Comparative Biochemistry and Physiology, Part B 2015 v.181 pp. 15-25
ISSN:: 1096-4959
Subject:: Macrobrachium; Western blotting; adenosine triphosphate; adenosinetriphosphatase; adults; diadromous fish; fluorescent antibody technique; freshwater; gene expression; juveniles; kinetics; magnesium; messenger RNA; ontogeny; protein synthesis; protons; shrimp; Amazon River
Abstract:: ... Novel kinetic properties of a microsomal gill V(H+)-ATPase from juvenile and adult Amazon River shrimp, Macrobrachium amazonicum, are described. While protein expression patterns are markedly different, Western blot analysis reveals a sole immunoreactive band, suggesting a single V(H+)-ATPase subunit isoform, distributed in membrane fractions of similar density in both ontogenetic stages. Immunofl ...
DOI:: 10.1016/j.cbpb.2014.11.002
: https://doi.org/10.1016/j.cbpb.2014.11.002

8. A methyl-deficient diet fed to rats during the pre- and peri-conception periods of development modifies the hepatic proteome in the adult offspring

Author:: Christopher A. Maloney; Susan M. Hay; Martin D. Reid; Gary Duncan; Fergus Nicol; Kevin D. Sinclair; William D. Rees
Source:: Genes & nutrition 2013 v.8 no.2 pp. 181-190
ISSN:: 1555-8932
Subject:: Western blotting; adults; amino acid metabolism; choline; diet; embryogenesis; energy metabolism; folic acid; gel electrophoresis; glucose; isoelectric point; liver; mass spectrometry; messenger RNA; post-translational modification; pregnancy; progeny; proteins; proteome; rats
Abstract:: ... A methyl-deficient diet (MD) lacking folic acid and the associated methyl donors choline and methionine, fed to the laboratory rat during the periods of oocyte and embryo development, has been shown to programme glucose metabolism in the offspring. The hepatic proteome of the male offspring of female rats fed MD diets for 3 weeks prior to mating and for the first 5 days of gestation has been exami ...
DOI:: 10.1007/s12263-012-0314-6
PubMed:: 22907820
PubMed Central:: PMC3575887
: https://doi.org/10.1007/s12263-012-0314-6

9. A proteomic analysis of Nipponia nippon (ID#162)

Author:: Mitsuru Oyanagi; Kentaro Kaneko; Yoshinori Kaneko; Maiko Sasaki; Chizuko Nishida; Yoichi Matsuda; Toshiaki Mitsui
Source:: Animal science journal = 2014 v.85 no.8 pp. 814-832
ISSN:: 1344-3941
Subject:: Gallus gallus; National Center for Biotechnology Information; Nipponia nippon; Western blotting; adults; antibodies; brain; databases; duodenum; heart; hens; liver; mass spectrometry; muscles; neck; polypeptides; proteome; proteomics; proventriculus; stomach
Abstract:: ... We investigated the proteome of a female Crested Ibis (Nipponia nippon, ID#162) that died on March 10, 2010 at the Sado Japanese Crested Ibis Conservation Center. Protein preparations from the brain, trachea, liver, heart, lung, proventriculus, muscular stomach, small intestine, duodenum, ovary and neck muscle were subjected to in‐solution shotgun mass spectrometry (MS)/MS analyses using an LTQ Or ...
DOI:: 10.1111/asj.12214
PubMed:: 24961376
: https://doi.org/10.1111/asj.12214

10. A rapid, targeted, neuron-selective, in vivo knockdown following a single intracerebroventricular injection of a novel chemically modified siRNA in the adult rat brain

Author:: Hidemitsu Nakajima; Takeya Kubo; Yuko Semi; Masanori Itakura; Mitsuru Kuwamura; Takeshi Izawa; Yasu-Taka Azuma; Tadayoshi Takeuchi
Source:: Journal of biotechnology 2012 v.157 no.2 pp. 326-333
ISSN:: 0168-1656
Subject:: RNA interference; Western blotting; adults; biotechnology; cerebellum; cortex; gene expression; glyceraldehyde-3-phosphate dehydrogenase; hippocampus; immunohistochemistry; inflammation; neurons; proteins; rats; small interfering RNA; toxicity; transfection
Abstract:: ... There has been a dramatic expansion of the literature on RNA interference and with it, increasing interest in the potential clinical utility of targeted inhibition of gene expression and associated protein knockdown. However, a critical factor limiting the experimental and therapeutic application of RNA interference is the ability to deliver small interfering RNAs (siRNAs), particularly in the cen ...
DOI:: 10.1016/j.jbiotec.2011.10.003
: https://doi.org/10.1016/j.jbiotec.2011.10.003

11. A salivary sheath protein essential for the interaction of the brown planthopper with rice plants

Author:: Hai-Jian Huang; Cheng-Wen Liu; Ye-Fang Cai; Min-Zhu Zhang; Yan-Yuan Bao; Chuan-Xi Zhang
Source:: Insect biochemistry and molecular biology 2015 v.66 pp. 77-87
ISSN:: 0965-1748
Subject:: Nilaparvata lugens; Oryza sativa; RNA interference; Western blotting; adults; alternative splicing; artificial diets; death; gels; genes; host plants; immunohistochemistry; insects; models; phenotype; rice; saliva; salivary glands; sap; staining; stylets
Abstract:: ... Salivary secretions, including gel saliva and watery saliva, play crucial roles in the interaction between the insect and plant during feeding. In this study, we identified a salivary gland-specific gene encoding a salivary sheath protein (NlShp) in Nilaparvata lugens. NlShp has two alternative splicing variants; both are expressed at high levels during the nymph and adult stages. Immunohistochemi ...
DOI:: 10.1016/j.ibmb.2015.10.007
: https://doi.org/10.1016/j.ibmb.2015.10.007

12. A splicing variant leads to complete loss of function of betaine–homocysteine methyltransferase (BHMT) gene in hepatocellular carcinoma

Author:: Hélène Pellanda; Farès Namour; Ma’atem Fofou-Caillierez; Aude Bressenot; Jean-Marc Alberto; Céline Chéry; Ahmet Ayav; Jean-Pierre Bronowicki; Jean-Louis Guéant; Thierry Forges
Source:: international journal of biochemistry & cell biology 2012 v.44 no.2 pp. 385-392
ISSN:: 1357-2725
Subject:: choline; hepatoma; Western blotting; betaine; adults; carcinogenesis; immunohistochemistry; methionine synthase; tissues; stop codon; human cell lines; humans; enzyme activity; exons; quantitative polymerase chain reaction; protein synthesis; liver; reverse transcriptase polymerase chain reaction; animal models; betaine-homocysteine S-methyltransferase; methionine
Abstract:: ... The remethylation of homocyteine into methionine is catalyzed either by methionine synthase (MTR) or by betaine–homocysteine methyltransferase (BHMT), in the liver. Choline/betaine deficiency and impaired BHMT pathway have been associated with hepatocellular carcinogenesis, in animal models. The molecular mechanisms that impair the BHMT pathway are unknown. We aimed to investigate BHMT, BHMT2, and ...
DOI:: 10.1016/j.biocel.2011.11.014
: https://doi.org/10.1016/j.biocel.2011.11.014

13. ATF3 Expression in the Corpus Luteum: Possible Role in Luteal Regression†

Author:: Dagan Mao; Xiaoying Hou; Heather Talbott; Robert Cushman; Andrea Cupp; John S. Davis
Source:: Molecular Endocrinology 2013 v.27 no.12 pp. 2066-2079
ISSN:: 0888-8809
Subject:: Western blotting; adults; cAMP-dependent protein kinase; cell nucleus; cell viability; cholesterol; cows; cyclic AMP; enzyme activity; gene expression; immunohistochemistry; in situ hybridization; in vitro studies; in vivo studies; luciferase; luteal cells; luteinizing hormone; messenger RNA; mitogen-activated protein kinase; phosphorylation; pregnancy; progesterone; prostaglandins; protein synthesis; puberty; quantitative polymerase chain reaction; secretion; transcription factors; tumor necrosis factors
Abstract:: ... The present study investigated the induction and possible role of activating transcription factor 3 (ATF3) in the corpus luteum. Postpubertal cattle were treated at midcycle with prostaglandin F2α(PGF) for 0-4 hours. Luteal tissue was processed for immunohistochemistry, in situ hybridization, and isolation of protein and RNA. Ovaries were also collected from midluteal phase and first-trimester pre ...
Handle:: 10113/59660
DOI:: 10.1210/me.2013-1274
PubMed:: 24196350
PubMed Central:: PMC3857195
: https://doi.org/10.1210/me.2013-1274

14. Aberrant fatty acid metabolism in skeletal muscle contributes to insulin resistance in zinc transporter 7 (znt7)-knockout mice

Author:: Liping Huang; Surapun Tepaamorndech; Catherine P. Kirschke; John W. Newman; William R. Keyes; Theresa L. Pedersen; Jureeporn Dumnil
Source:: Journal of Biological Chemistry 2018 v.293 no.20 pp. 7549-7563
ISSN:: 0021-9258
Subject:: Golgi apparatus; Western blotting; adults; chemistry; fatty acid metabolism; fatty acid-binding proteins; immunohistochemistry; inflammation; insulin resistance; males; mitochondria; models; muscles; oxidative stress; oxylipins; phenotype; protein synthesis; skeletal muscle; zinc
Abstract:: ... ZnT7 (Slc30a7) is a widely expressed zinc transporter involved in sequestration of zinc into the Golgi apparatus and vesicular compartments. znt7-knockout (KO) mice are mildly zinc-deficient and lean. Despite their lean phenotype, adult male znt7-KO mice are prone to insulin resistance. We hypothesized that fat partitioning from adipose to nonadipose tissues causes insulin resistance in znt7-KO mi ...
Handle:: 10113/7344772
DOI:: 10.1074/jbc.m117.817692
CHORUS:: 10.1074/jbc.m117.817692
: https://doi.org/10.1074/jbc.m117.817692

15. Acid-sensing ion channels (ASICs) 2 and 4.2 are expressed in the retina of the adult zebrafish

Author:: E. Viña; V. Parisi; C. Sánchez-Ramos; R. Cabo; M. C. Guerrera; L. M. Quirós; A. Germanà; J. A. Vega; O. García-Suárez
Source:: Cell and tissue research 2015 v.360 no.2 pp. 223-231
ISSN:: 0302-766X
Subject:: Danio rerio; Western blotting; adults; animal models; ganglia; immunohistochemistry; in situ hybridization; ion channels; mammals; messenger RNA; nerve tissue; nociception; photoreceptors; polymerase chain reaction; retina; synaptic transmission
Abstract:: ... Acid-sensing ion channels (ASICs) are H⁺-gated, voltage-insensitive cation channels involved in synaptic transmission, mechanosensation and nociception. Different ASICs have been detected in the retina of mammals but it is not known whether they are expressed in adult zebrafish, a commonly used animal model to study the retina in both normal and pathological conditions. We study the expression and ...
DOI:: 10.1007/s00441-014-2084-5
: https://doi.org/10.1007/s00441-014-2084-5

16. Activation of calcium-sensing receptor increases TRPC3 expression in rat cardiomyocytes

Author:: Shan-Li Feng; Ming-Rui Sun; Ting-Ting Li; Xin Yin; Chang-Qing Xu; Yi-Hua Sun
Source:: Biochemical and biophysical research communications 2011 v.406 no.2 pp. 278-284
ISSN:: 0006-291X
Subject:: messenger RNA; confocal microscopy; sarcoplasmic reticulum; calcium; spermine; reverse transcriptase polymerase chain reaction; cardiomyocytes; Western blotting; adults; rats; receptors; gadolinium; apoptosis
Abstract:: ... Transient receptor potential (TRP) channels are expressed in cardiomyocytes, which gate a type of influx of extracellular calcium, the capacitative calcium entry. TRP channels play a role in mediating Ca²⁺ overload in the heart. Calcium-sensing receptors (CaR) are also expressed in rat cardiac tissue and promote the apoptosis of cardiomyocytes by Ca²⁺ overload. However, data about the link between ...
DOI:: 10.1016/j.bbrc.2011.02.033
: https://doi.org/10.1016/j.bbrc.2011.02.033

17. Activation of the Wnt/β-catenin signaling pathway is associated with glial proliferation in the adult spinal cord of ALS transgenic mice

Author:: Yanchun Chen; Yingjun Guan; Huancai Liu; Xin Wu; Li Yu; Shanshan Wang; Chunyan Zhao; Hongmei Du; Xin Wang
Source:: Biochemical and biophysical research communications 2012 v.420 no.2 pp. 397-403
ISSN:: 0006-291X
Subject:: Western blotting; adults; astrocytes; cell membranes; cell proliferation; fluorescent antibody technique; gene expression regulation; gene therapy; genes; genetically modified organisms; labeling techniques; messenger RNA; mice; motor neurons; neurodegenerative diseases; reverse transcriptase polymerase chain reaction; sclerosis; signal transduction; spinal cord
Abstract:: ... Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by the progressive and fatal loss of motor neurons. In ALS, there is a significant cell proliferation in response to neurodegeneration; however, the exact molecular mechanisms of cell proliferation and differentiation are unclear. The Wnt signaling pathway has been shown to be involved in neurodegenerative processes. ...
DOI:: 10.1016/j.bbrc.2012.03.006
: https://doi.org/10.1016/j.bbrc.2012.03.006

18. Acute effects of ethanol on feeding behavior and leptin-induced STAT3 phosphorylation in rat hypothalamus

Author:: Fujita, N.; Sakamaki, H.; Uotani, S.; Takahashi, R.; Kuwahara, H.; Kita, A.; Oshima, K.; Yamasaki, H.; Yamaguchi, Y.; Eguchi, K.
Source:: International journal of obesity 2003 v.27 no.1 pp. 55-59
ISSN:: 0307-0565
Subject:: Western blotting; acute effects; adults; appetite; body weight; drinking; energy balance; ethanol; feeding behavior; food intake; hypothalamus; intraperitoneal injection; leptin; phosphorylation; rats; satiety; signal transduction; transcription factors; tyrosine
Abstract:: ... OBJECTIVE: Drinking ethanol stimulates the appetite, producing a positive energy balance. The mechanism by which ethanol regulates the appetite in the central nervous system, however, has not been fully understood. The aim of this study is to investigate the interaction of ethanol with the satiety effect of leptin, a hormone which suppresses the appetite in the hypothalamic region. DESIGN: Leptin ...
DOI:: 10.1038/sj.ijo.0802194
PubMed:: 12532154
: https://doi.org/10.1038/sj.ijo.0802194

19. Adverse Effects of Rice gall dwarf virus upon its Insect Vector Recilia dorsalis (Hemiptera: Cicadellidae)

Author:: Chen Yong; Lu Chengcong; Li Manman; Wu Wei; Zhou Guohui; Wei Taiyun
Source:: Plant disease 2016 v.100 no.4 pp. 784-790
ISSN:: 0191-2917
Subject:: life history; Western blotting; insect vectors; Rice gall dwarf virus; adults; longevity; fecundity; viruses; vitellogenin; gene expression regulation; females; crop production; monitoring; eggs; adverse effects; overwintering; survival rate; Recilia dorsalis; reverse transcriptase polymerase chain reaction; South East Asia; China
Abstract:: ... Rice gall dwarf virus (RGDV), a plant reovirus that threatens rice production in Southeast Asia and Southern China, is transmitted by the leafhopper vector Recilia dorsalis in a persistent-propagative manner. To assess the direct effects of RGDV on R. dorsalis, we established an infected leafhopper population from eggs laid by viruliferous females using the water-soaked filter paper culture method ...
DOI:: 10.1094/PDIS-06-15-0713-RE
: https://doi.org/10.1094/PDIS-06-15-0713-RE

20. Age-related changes in the morphology and protein expression of the thymus of healthy yaks (Bos grunniens)

Author:: Qian Zhang; Kun Yang; Pan Yangyang; Junfeng He; Sijiu Yu; Yan Cui
Source:: American journal of veterinary research 2016 v.77 no.6 pp. 567-574
ISSN:: 0002-9645
Subject:: Western blotting; adults; caspase-3; connective tissues; cortex; dendritic cells; euthanasia; humans; immunohistochemistry; juveniles; males; messenger RNA; neonates; plateaus; protein synthesis; quantitative polymerase chain reaction; staining; thymocytes; thymus gland; transmission electron microscopy; yaks
Abstract:: ... OBJECTIVE To evaluate age-related changes in the morphology and expression of cluster of differentiation 3 (CD3), S100 β, and caspase-3 of the thymus of healthy yaks (Bos grunniens). ANIMALS 15 healthy male yaks of various ages from highland plateaus. PROCEDURES Yaks were allocated to 3 groups on the basis of age (newborn [1 to 7 days old; n = 5], juvenile [5 to 7 months old, 5], and adult [3 to 4 ...
DOI:: 10.2460/ajvr.77.6.567
: https://doi.org/10.2460/ajvr.77.6.567

21. Aging and retinoid X receptor agonists on masculinization of female Pomacea canaliculata, with a critical appraisal of imposex evaluation in the Ampullariidae

Author:: Maximiliano Giraud-Billoud; Alfredo Castro-Vazquez
Source:: Ecotoxicology and environmental safety 2019 v.169 pp. 573-582
ISSN:: 0147-6513
Subject:: Pomacea canaliculata; Western blotting; adults; agonists; females; masculinization; peroxisome proliferator-activated receptor gamma; retinoic acid; sex differentiation disorders; tributyltin
Abstract:: ... Ampullariidae are unique among gastropods in that females normally show a primordium of the copulatory apparatus (CApp). The aims of this study were (a) to quantitatively evaluate the development and growth of the female CApp with age; (b) to compare the effects of RXR and PPARγ agonists in adult females of known age and (c) to explore the effect of masculinizing RXR agonists on the expression of ...
DOI:: 10.1016/j.ecoenv.2018.10.096
: https://doi.org/10.1016/j.ecoenv.2018.10.096

22. Alteration in iron efflux affects male sex hormone testosterone biosynthesis in a diet-induced obese rat model

Author:: Adi Lukas KurniawanThese authors contributed equally to this work.; Yu-Chieh Lee; Chun-Kuang Shih; Rong-Hong Hsieh; Seu-Hwa Chen; Jung-Su Chang
Source:: Food & function 2019 v.10 no.7 pp. 4113-4123
ISSN:: 2042-650X
Subject:: Western blotting; adults; animal disease models; apoptosis; biogenesis; biosynthesis; diet; germ cells; hepcidin; iron; iron overload; laboratory animals; lipid content; males; men; mitochondria; obesity; proteins; rats; staining; testes; testosterone; therapeutics
Abstract:: ... This study was motivated by clinical observations that dysmetabolic iron overload syndrome (DIOS) and an androgen deficiency are common features observed in obese adult men; however, the molecular mechanism underlying the effects of DIOS on androgen deficiency remains to be elucidated. We established a DIOS animal model by feeding Sprague-Dawley rats an iron/fat-enriched diet (50% fat plus 0.25, 1 ...
DOI:: 10.1039/c8fo01870g
: https://doi.org/10.1039/c8fo01870g

23. Alterations of protein expression in serum of infants with intrauterine growth restriction and different gestational ages

Author:: María D. Ruis-González; María D. Cañete; José L. Gómez-Chaparro; Nieves Abril; Ramón Cañete; Juan López-Barea
Source:: Journal of proteomics 2015 v.119 pp. 169-182
ISSN:: 1874-3919
Subject:: Western blotting; adults; albumins; apolipoprotein E; blood groups; blood serum; brain; cytoskeleton; gestational age; image analysis; keratin; morbidity; neonates; protein synthesis; proteome; solutes; transferrin
Abstract:: ... Intrauterine growth restriction (IUGR) is associated with increased morbidity and metabolic anomalies in adults. The serum proteome of venous blood was compared in 43 IUGR and 45 adequate gestational age (AGA) infants, separated into three gestational age groups, “Very Preterm” 29–32weeks, “Moderate Preterm” 33–36w, and, “Term” ≥37weeks, in samples drawn three times from birth to 1month of life. A ...
DOI:: 10.1016/j.jprot.2015.02.003
: https://doi.org/10.1016/j.jprot.2015.02.003

24. Ameliorative effect of Gastrodia elata Blume extracts on depression in zebrafish and cellular models through modulating reticulon 4 receptors and apoptosis

Author:: Rongchun Wang; Qingyu Ren; Daili Gao; Yam Nath Paudel; Xia Li; Lizhen Wang; Pengyu Zhang; Baokun Wang; Xueliang Shang; Meng Jin
Source:: Journal of ethnopharmacology 2022 v.289 pp. 115018
ISSN:: 0378-8741
Subject:: Danio rerio; Gastrodia elata; Oriental traditional medicine; Western blotting; adults; antidepressants; apoptosis; cell viability; diet; fluorescent antibody technique; gene expression; models; pharmacology; protein synthesis; China
Abstract:: ... Gastrodia elata Blume (G. elata), a traditional Chinese herb, known as “Tian Ma”, is widely used as a common medicine and diet ingredient for treating or preventing neurological disorders for thousands of years in China. However, the anti-depressant effect of G. elata and the underlying mechanism have not been fully evaluated. The study is aimed to investigate the anti-depressant effect and the mo ...
DOI:: 10.1016/j.jep.2022.115018
: https://doi.org/10.1016/j.jep.2022.115018

25. Amphibian larvae and zinc sulphate: a suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium

Author:: Yovanovich, Carola A. M.; Jungblut, Lucas D.; Heer, Tamara; Pozzi, Andrea G.; Paz, Dante A.
Source:: Cell and tissue research 2009 v.336 no.1 pp. 1-9
ISSN:: 0302-766X
Subject:: Western blotting; adults; amphibians; antiserum; epithelium; experimental design; image analysis; immunohistochemistry; models; neurons; tadpoles; zinc sulfate
Abstract:: ... The vertebrate olfactory system has fascinated neurobiologists over the last six decades because of its ability to replace its neurons and synaptic connections continuously throughout adult life, under both physiological and pathological conditions. Among the factors that are proposed to be involved in this regenerative potential, brain-derived neurotrophic factor (BDNF) is a candidate for having ...
DOI:: 10.1007/s00441-009-0752-7
PubMed:: 19221803
: https://doi.org/10.1007/s00441-009-0752-7

26. An antigenic recombinant serine protease from Trichinella spiralis induces protective immunity in BALB/c mice

Author:: Xin Li; Jian-ping Yao; Ai-hua Pan; Wei Liu; Xu-chu Hu; Zhong-dao Wu; Xing-wang Zhou
Source:: Parasitology research 2013 v.112 no.9 pp. 3229-3238
ISSN:: 0932-0113
Subject:: Escherichia coli; Trichinella spiralis; Western blotting; adults; antibodies; antigens; chromatography; complementary DNA; genes; immune response; immunization; immunoglobulin E; immunoglobulin G; mice; muscle larvae; neonates; polymerase chain reaction; serine proteinases
Abstract:: ... In this study, we report the cloning and characterization of a cDNA encoding a Trichinella serine protease gene (TspSP-1.3) from GenBank. The recombinant TspSP-1.3 protein (rTspSP-1.3) was expressed in an Escherichia coli expression system and purified with Ni-affinity chromatography. Real-time quantitative PCR analysis revealed that TspSP-1.3 was expressed at significantly higher levels in muscle ...
DOI:: 10.1007/s00436-013-3500-6
PubMed:: 23828191
: https://doi.org/10.1007/s00436-013-3500-6

27. Analysis of OCT4 expression in an extended panel of human tumor cell lines from multiple entities and in human mesenchymal stem cells

Author:: Mueller, T.; Luetzkendorf, J.; Nerger, K.; Schmoll, H.-J.; Mueller, L. P.
Source:: Cellular and molecular life sciences 2009 v.66 no.3 pp. 495-503
ISSN:: 1420-682X
Subject:: Western blotting; adults; embryonic stem cells; germ cells; humans; immunocytochemistry; immunohistochemistry; neoplasm cells; reverse transcriptase polymerase chain reaction
Abstract:: ... OCT4 is considered a main regulator of embryonic stem cell pluripotency and self renewal capacity. It was shown that relevant OCT4 expression only occurs in cells of embryonic pluripotent nature. However, several recent publications claimed to have demonstrated OCT4 expression in human somatic tumor cells, human adult stem or progenitor cells and differentiated cells.We analysed 42 human tumor cel ...
DOI:: 10.1007/s00018-008-8623-z
: https://doi.org/10.1007/s00018-008-8623-z

28. Analysis of an expressed sequence tag library from Dicrocoelium dentriticum

Author:: A.M. Martínez-Ibeas; M.J. Perteguer; C. González-Lanza; T. Gárate; M.Y. Manga-González
Source:: Experimental parasitology 2013 v.135 no.2 pp. 287-296
ISSN:: 0014-4894
Subject:: parasites; bacteriophages; Western blotting; parasitology; ruminants; adults; recombinant antigens; recombinant proteins; financial economics; enzyme-linked immunosorbent assay; gene expression; screening; databases; Escherichia coli; Dicrocoelium dendriticum; messenger RNA; complementary DNA; cDNA libraries; protein synthesis; serodiagnosis; vaccines; expressed sequence tags; liver diseases
Abstract:: ... Dicrocoeliosis caused by Dicrocoelium dendriticum is an important liver disease, which affects ruminants all around the world. Despite the significant economic losses caused by this trematode, molecular knowledge is very scarce. In fact, there is no information in the expressed sequence tag (EST) database about the parasite. Furthermore, the immunological diagnosis of dicrocoeliosis remains unsati ...
DOI:: 10.1016/j.exppara.2013.07.005
: https://doi.org/10.1016/j.exppara.2013.07.005

29. Analysis of the developmental expression of small VCP-interacting protein and its interaction with steroidogenic acute regulatory protein in Leydig cells

Author:: Gulben Akcan; Ebru Alimogullari; Radwan Abu-Issa; Sevil Cayli
Source:: Reproductive Biology 2020 v.20 no.1 pp. 88-96
ISSN:: 1642-431X
Subject:: Leydig cells; Sertoli cells; Western blotting; adults; amino acids; androgens; cytoplasm; droplets; immunohistochemistry; lipids; mice; protein degradation; protein synthesis; puberty; small interfering RNA; staining; steroidogenic acute regulatory protein
Abstract:: ... Small VCP-interacting protein (SVIP) is a 9-kDa protein that is composed of 76 amino acids, and it plays a role in the endoplasmic reticulum-associated protein degradation (ERAD) pathway. Recent studies have shown that SVIP is an androgen-responsive protein and its expression is regulated by androgens. Because no data are available regarding the cellular localization and expression of SVIP in the ...
DOI:: 10.1016/j.repbio.2020.01.006
: https://doi.org/10.1016/j.repbio.2020.01.006

30. Analysis of the stem cell characteristics of adult stem cells from Arbas white Cashmere goat

Author:: Yu Ren; Haiqing Wu; Xiao Wang; Na Xue; Hao Liang; Dongjun Liu
Source:: Biochemical and biophysical research communications 2014 v.448 pp. 121-128
ISSN:: 0006-291X
Subject:: GATA transcription factors; Western blotting; adult stem cells; adults; appendages; biomarkers; bone marrow; embryonic stem cells; gene expression; goats; immunocytochemistry; in vitro culture; interleukin-6; messenger RNA; mice; neoplasms; reverse transcriptase polymerase chain reaction
Abstract:: ... Studies have shown that multipotent adult stem cells possess differentiation characteristics similar to embryonic stem cells and pluripotent stem cells. We aimed to explore these similarities further by examining the expression of the pluripotency and stemness biomarkers, AKP, IL-6, Nanog, Oct-4, Rex-1, Sox-2 and TERT, as well as the triploblastic biomarkers, Sox-1, Myod1 and Gata-6 in adipose-der ...
DOI:: 10.1016/j.bbrc.2013.12.044
: https://doi.org/10.1016/j.bbrc.2013.12.044

31. Analysis of the structural proteins from the Musca domestica hytrosavirus with an emphasis on the major envelope protein

Author:: D.G. Boucias; F. Deng; Z. Hu; A. Garcia-Maruniak; V.-U. Lietze
Source:: Journal of invertebrate pathology 2013 v.112 pp. S44
ISSN:: 0022-2011
Subject:: Musca domestica; Western blotting; adults; antibodies; bioassays; electron microscopy; hypertrophy; molecular weight; neutralization; nuclear membrane; nucleocapsid; open reading frames; polyacrylamide gel electrophoresis; rough endoplasmic reticulum; salivary glands; structural proteins; viral antigens; viruses
Abstract:: ... The Musca domestica hytrosavirus (MdHV), a member of the family Hyrosaviridae, is a large, dsDNA, enveloped virus that infects adult house flies and causes a diagnostic hypertrophy of the salivary gland. Herein, studies were directed at identifying key structural components of the viral envelope and nucleocapsid. SDS-PAGE of detergent-treated virus fractions identified protein bands unique to the ...
DOI:: 10.1016/j.jip.2012.03.016
: https://doi.org/10.1016/j.jip.2012.03.016

32. Androgen receptor, aromatase, oestrogen receptor α/β and G protein‐coupled receptor 30 expression in the testes and epididymides of adult sheep

Author:: Wenbo Ge; Longfei Xiao; Hongwei Duan; Yuting Jiang; Jianshu Lv; Ziqiang Ding; Junjie Hu; Xingxu Zhao; Yong Zhang
Source:: Reproduction in domestic animals 2020 v.55 no.4 pp. 460-468
ISSN:: 0936-6768
Subject:: G-protein coupled receptors; Leydig cells; Western blotting; adults; androgen receptors; androgens; animal reproduction; aromatase; cell proliferation; epididymis; epithelium; estradiol; estrogen receptors; immunohistochemistry; reverse transcriptase polymerase chain reaction; sexual maturity; sheep; smooth muscle; spermatozoa
Abstract:: ... The androgen receptor (AR) plays a key role in reproduction, and aromatase (P450arom), nuclear oestrogen receptors (ERs) α and β, and G protein‐coupled receptor 30 (GPR30) are important for testicular and epididymal cell proliferation and development. In the study, we have investigated the expression and localization of AR, P450arom, ERα, ERβ and GPR30 in testes and epididymides of sexually mature ...
DOI:: 10.1111/rda.13638
: https://doi.org/10.1111/rda.13638

33. Angiogenic properties of adult human thymus fat

Author:: Salas, Julián; Montiel, Mercedes; Jiménez, Eugenio; Valenzuela, Miguel; Valderrama, José Francisco; Castillo, Rafael; González, Sergio; El Bekay, Rajaa
Source:: Cell and tissue research 2009 v.338 no.2 pp. 313-318
ISSN:: 0302-766X
Subject:: Western blotting; adipose tissue; adults; angiogenesis; cell movement; endothelial cells; genes; humans; immunohistochemistry; lipogenesis; protein synthesis; proteins; quantitative polymerase chain reaction; umbilical cord; vascular endothelial growth factor A
Abstract:: ... The endogenous proangiogenic properties of adipose tissue are well recognized. Although the adult human thymus has long been known to degenerate into fat tissue, it has never been considered as a potential source of angiogenic factors. We have investigated the expression of diverse angiogenic factors, including vascular endothelial growth factor A and B, angiopoietin 1, and tyrosine-protein kinase ...
DOI:: 10.1007/s00441-009-0867-x
PubMed:: 19760288
: https://doi.org/10.1007/s00441-009-0867-x

34. Anoctamin 1 expression in the mouse auditory brainstem

Author:: Sang Jae Cho; Ji Hyun Jeon; Do Ill Chun; Sang Won Yeo; In-Beom Kim
Source:: Cell and tissue research 2014 v.357 no.3 pp. 563-569
ISSN:: 0302-766X
Subject:: RNA-directed DNA polymerase; Western blotting; adults; brain stem; chloride channels; ganglia; immunohistochemistry; mice; polymerase chain reaction; retina; synaptic transmission
Abstract:: ... Calcium-activated chloride channels (CaCCs) are involved in numerous physiological functions, including the epithelial movement of fluid. Anoctamin 1 (ANO1) has recently been cloned and characterized as a CaCC and is known to be expressed in various secretory epithelia and in nervous tissues such as the dorsal root ganglia and retina. However, data regarding the expression, function, and cellular ...
DOI:: 10.1007/s00441-014-1897-6
PubMed:: 24853671
: https://doi.org/10.1007/s00441-014-1897-6

35. Anti-Müllerian hormone receptor type 2 (AMHR2) expression in bovine oviducts and endometria: comparison of AMHR2 mRNA and protein abundance between old Holstein and young and old Wagyu females

Author:: Raihana Nasrin Ferdousy; Onalenna Kereilwe; Hiroya Kadokawa
Source:: Reproduction, fertility, and development 2020 v.32 no.8 pp. 738-747
ISSN:: 1031-3613
Subject:: Holstein; Wagyu; Western blotting; adults; anti-Mullerian hormone; antibodies; cows; endometrium; glycoproteins; gonadotropins; granulosa cells; hormone receptors; hormone secretion; immunohistochemistry; messenger RNA; mucosa; oviducts; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction
Abstract:: ... Anti-Müllerian hormone (AMH) is a glycoprotein produced by granulosa cells of preantral and small antral follicles that has multiple important roles in the ovaries. Recent studies have revealed extragonadal AMH regulation of gonadotrophin secretion from bovine gonadotrophs. In this study we investigated whether the primary receptor for AMH, AMH receptor type 2 (AMHR2), is expressed in bovine ovidu ...
DOI:: 10.1071/RD19121
: https://doi.org/10.1071/RD19121

36. Aquaporin AQP11 in the testis: molecular identity and association with the processing of residual cytoplasm of elongated spermatids

Author:: Yeung, C.H.; Cooper, T.G.
Source:: Reproduction 2010 v.139 no.1 pp. 209-216
ISSN:: 1470-1626
Subject:: Sertoli cells; Western blotting; adults; aquaporins; databases; gene banks; gene expression; humans; immunohistochemistry; kidneys; messenger RNA; mice; nucleotides; open reading frames; organelles; polymerase chain reaction; protein synthesis; rats; spermatids; spermatogenesis; spermatozoa; spermiation; tail
Abstract:: ... AQP11 is one of the latest aquaporin (AQP) family members found, which differs from the other AQPs by its intracellular localisation and unusual water pore nucleotides with unclear function. Despite the highest mRNA expression among organs having been reported in the testis, the testicular molecule has not been studied in detail. Immunohistochemistry of rat adult testis localised AQP11 to the elon ...
DOI:: 10.1530/REP-09-0298
PubMed:: 19812234
: https://doi.org/10.1530/REP-09-0298

37. ArHsp22, a developmentally regulated small heat shock protein produced in diapause-destined Artemia embryos, is stress inducible in adults

Author:: Qiu, Zhijun; MacRae, Thomas H.
Source:: FEBS journal 2008 v.275 no.14 pp. 3556-3566
ISSN:: 1742-464X
Subject:: Artemia franciscana; Western blotting; adults; arginine; citrate (si)-synthase; denaturation; diapause; gene expression; heat shock proteins; insulin; quantitative polymerase chain reaction; stress tolerance; thermal stress
Abstract:: ... Diapause embryos of the crustacean Artemia franciscana exhibit extreme stress tolerance, a property thought to involve molecular chaperones known as small heat shock proteins. To further explore this idea, the structure, function and synthesis of ArHsp22, an Artemia small heat shock protein, were characterized. ArHsp22 contains amino-terminal WXDPF motifs, an α-crystallin domain with a highly cons ...
DOI:: 10.1111/j.1742-4658.2008.06501.x
: https://doi.org/10.1111/j.1742-4658.2008.06501.x

38. Arginine kinase from Haemonchus contortus decreased the proliferation and increased the apoptosis of goat PBMCs in vitro

Author:: Muhammad Ehsan; WenXiang Gao; Javaid Ali Gadahi; MingMin Lu; XinChao Liu; YuJian Wang; RuoFeng Yan; LiXin Xu; XiaoKai Song; XiangRui Li
Source:: Parasites & vectors 2017 v.10 no.1 pp. 311
ISSN:: 1756-3305
Subject:: Escherichia coli; Haemonchus contortus; Western blotting; adults; affinity chromatography; apoptosis; arginine kinase; cell movement; cell proliferation; digestive system; dose response; enzyme activity; fluorescent antibody technique; genes; goats; immune response; immunohistochemistry; immunomodulators; interferon-gamma; interleukin-10; interleukin-17; interleukin-4; invertebrates; mononuclear leukocytes; nitric oxide; open reading frames; rats; recombinant proteins; reverse transcriptase polymerase chain reaction; transforming growth factor beta 1
Abstract:: ... BACKGROUND: Arginine kinase (AK), an important member of phosphagen kinase family has been extensively studied in various vertebrates and invertebrates. Immunologically, AKs are important constituents of different body parts, involved in various biological and cellular functions, and considered as immune-modulator and effector for pro-inflammatory cytokines. However, immunoregulatory changes of ho ...
DOI:: 10.1186/s13071-017-2244-z
PubMed:: 28651566
PubMed Central:: PMC5485575
: https://doi.org/10.1186/s13071-017-2244-z

39. Aristoyunnolin H attenuates extracellular matrix secretion in cardiac fibroblasts by inhibiting calcium influx

Author:: Shao-rui Chen; Wen-ping Zhang; Jing-mei Bao; Zhong-bin Cheng; Sheng Yin
Source:: Archives of pharmacal research 2017 v.40 no.1 pp. 122-130
ISSN:: 0253-6269
Subject:: Aristolochia; Oriental traditional medicine; Western blotting; adults; angiotensin II; calcium; calcium signaling; collagen; extracellular matrix; fibroblasts; fibronectins; phosphorylation; protein kinases; rats; secretion; sesquiterpenoids
Abstract:: ... Aristoyunnolin H is a novel aristophyllene sesquiterpenoid isolated from the traditional Chinese medicine Aristolochia yunnanensis Franch. The present research was designed to explore the anti-fibrotic effects of aristoyunnolin H in adult rat cardiac fibroblasts (CFs) stimulated with angiotensin II (Ang II). Western blot analysis data showed that aristoyunnolin H reduced the upregulation of fibron ...
DOI:: 10.1007/s12272-016-0843-4
: https://doi.org/10.1007/s12272-016-0843-4

40. Astragaloside IV inhibits Angiotensin II-stimulated proliferation of rat vascular smooth muscle cells via the regulation of CDK2 activity

Author:: Deng-qing Zhang; Jin-song Li; Yu-mei Zhang; Feng Gao; Ruo-zhu Dai
Source:: Life sciences 2018 v.200 pp. 105-109
ISSN:: 0024-3205
Subject:: Oriental traditional medicine; Western blotting; actin; adults; angiotensin II; astragalosides; atherosclerosis; cell cycle; cyclin-dependent kinase; enzyme activity; flow cytometry; muscles; myocytes; phenotype; rats; reverse transcriptase polymerase chain reaction; smooth muscle
Abstract:: ... Astragaloside IV (AS-IV) is the central active component extracted from Radix astragali, an herbal remedy widely used in traditional Chinese medicine for the treatment of cardiovascular diseases. Aberrant proliferation of vascular smooth muscle cells (VSMCs) is closely involved in the initiation and progression of cardiovascular complications, such as atherosclerosis. Here we investigated whether ...
DOI:: 10.1016/j.lfs.2018.03.036
: https://doi.org/10.1016/j.lfs.2018.03.036

41. Autophagic activity in the mouse urinary bladder urothelium as a response to starvation

Author:: Andreja Erman; Nataša Resnik; Rok Romih
Source:: Protoplasma 2013 v.250 no.1 pp. 151-160
ISSN:: 0033-183X
Subject:: Western blotting; adults; autophagy; bladder; homeostasis; mice; neonates; quantitative analysis; starvation; toxicity; urine; vacuoles
Abstract:: ... The urinary bladder urothelium is subjected to mechanical forces during cycles of distension and contraction, and its superficial cells are constantly flushed by toxic urine. Yet, the urothelium shows a very slow turnover of cells and superficial cells are extremely long lived. Autophagy has a well-known role in tissue homeostasis and serves as a protective mechanism against cellular stress. There ...
DOI:: 10.1007/s00709-012-0387-5
PubMed:: 22407469
: https://doi.org/10.1007/s00709-012-0387-5

42. Autophagy can repair endoplasmic reticulum stress damage of the passive Heymann nephritis model as revealed by proteomics analysis

Author:: Liyuan Wang; Quan Hong; Yang Lv; Zhe Feng; Xueguang Zhang; Lingling Wu; Shaoyuan Cui; Kai Hou; Huabin Su; Zhiyong Huang; Di Wu; Xiangmei Chen
Source:: Journal of proteomics 2012 v.75 no.13 pp. 3866-3876
ISSN:: 1874-3919
Subject:: Western blotting; adults; animal models; autophagy; cytoskeleton; endoplasmic reticulum; fluorescent antibody technique; nephritis; nephrotic syndrome; pathogenesis; proteins; proteome; proteomics; tunicamycin
Abstract:: ... Membranous nephropathy is a common cause of nephrotic syndrome in adults. Although many mechanisms have been proposed, whole proteomic research is still lacking. We analyzed the passive Heymann nephritis animal model using label-free quantitative proteome technology. Results showed 160 differential proteins between control and PHN model groups at days 14 and 21. The expression level of endoplasmic ...
DOI:: 10.1016/j.jprot.2012.04.016
PubMed:: 22595305
: https://doi.org/10.1016/j.jprot.2012.04.016

43. Autophagy of hepatic stellate cell induced by Clonorchis sinensis

Author:: Bao Zheng; Zhiyan Gao; Liumei Liang; Yunyu Lu; Yongting Kong; Wanting Chen; Keying Lin; Wanqi Chen; Jingying Mai; Yanwen Li; Changling Ma
Source:: Molecular biology reports 2022 v.49 no.3 pp. 1895-1902
ISSN:: 0301-4851
Subject:: Clonorchis sinensis; Western blotting; adults; autophagosomes; autophagy; cell proliferation; chloroquine; collagen; humans; liver cirrhosis; molecular biology; parasites; pathogenesis; plasmids; risk factors; secretion; tetrazolium
Abstract:: ... BACKGROUND: Clonorchis sinensis was a food-borne zoonotic parasite in the worldwide and also an important risk factor of hepatic fibrosis. Excretory/secretion products of C. sinensis (CsESPs) are involved in parasite-host interactions and contribute to the development of hepatic damage. The aim of the present study was to investigate whether CsESPs and CsTP (adult protein) could induce autophagy o ...
DOI:: 10.1007/s11033-021-07001-9
: https://doi.org/10.1007/s11033-021-07001-9

44. BRCA-1 Gene Expression and Comparative Proteomic Profile of Primordial Follicles from Young and Adult Buffalo (Bubalus bubalis) Ovaries

Author:: Vijayakumar Govindaraj; Harshini Krishnagiri; Manmohan Singh Chauhan; A. J. Rao
Source:: Animal biotechnology 2017 v.28 no.2 pp. 94-103
ISSN:: 1532-2378
Subject:: Bubalus bubalis; messenger RNA; DNA damage; buffaloes; protein synthesis; cell viability; humans; tumor suppressor proteins; reverse transcriptase polymerase chain reaction; Western blotting; gene expression; genes; DNA repair; mitochondria; rats; proteomics; gene expression regulation; oocytes; adults
Abstract:: ... In our previous study, we demonstrated that the repair efficiency of DNA double-strand breaks declines with increasing age in rat primordial follicles. In the present study, we extended our studies to buffalo (Bubalus bubalis) wherein we studied the expression of BRCA-1 related DNA repair genes in primordial follicles of young (12 months-22 months) and adult (72–96 months) buffaloes. The relative ...
DOI:: 10.1080/10495398.2016.1210613
: https://doi.org/10.1080/10495398.2016.1210613

45. Berberine protects against ischemia/reperfusion injury after orthotopic liver transplantation via activating Sirt1/FoxO3α induced autophagy

Author:: Yuanbang Lin; Mingwei Sheng; Yiqi Weng; Rubin Xu; Ning Lu; Hongyin Du; Wenli Yu
Source:: Biochemical and biophysical research communications 2017 v.483 pp. 885-891
ISSN:: 0006-291X
Subject:: Western blotting; acetylation; adults; apoptosis; autophagy; berberine; gene expression; histopathology; ischemia; liver; liver function; liver transplant; males; messenger RNA; oxidative stress; precipitin tests; protective effect; rats; transmission electron microscopy
Abstract:: ... The effects and mechanism of berberine (BBR) on hepatic injury after orthotopic liver transplantation (OLT) have not been well characterized. We examined the role of Sirt1/FoxO3α axis in the protective effect of BBR on ischemia/reperfusion injury after OLT. Adult male Wistar rats were randomly allocated into four groups: Sham, OLT, OLT with BBR pretreatment (BBR), OLT with BBR and Sirt1 inhibitor ...
DOI:: 10.1016/j.bbrc.2017.01.028
: https://doi.org/10.1016/j.bbrc.2017.01.028

46. Bio-guided identification of proteins for the diagnosis of cysticercosis in swine

Author:: Priscilla Nativel; Anjanirina Rahantamalala; Sitraka Ramiandrisoa; Virginie Rasoamampianinaa; Magalie Duchateau; Julia Chamot-Rooke; Remy Guebey; Harentsoaniaina Rasamoelina-Andriamanivo; Ronan Jambou
Source:: Veterinary parasitology 2016 v.220 pp. 23-27
ISSN:: 0304-4017
Subject:: Taenia solium; Western blotting; adults; antigens; brain; cysticerci; diagnostic techniques; disease control; eggs; enzyme-linked immunosorbent assay; expressed sequence tags; feces; food contamination; gels; humans; immunoaffinity chromatography; ingestion; ion exchange chromatography; larvae; mass spectrometry; neurocysticercosis; proteins; rural areas; seizures; swine; tapeworms
Abstract:: ... Neurocysticercosis (NCC) is one of the most prevalent parasitic infection of the brain and the most common cause of seizures in adults in tropical countries. Cysticercosis is caused by larvae of Taenia solium, a human tapeworm. Pig or humans are infected by ingestion of eggs in food contaminated by human feces. Diagnosis and treatment of pigs is a pillar of the control of the disease in a country. ...
DOI:: 10.1016/j.vetpar.2016.02.021
: https://doi.org/10.1016/j.vetpar.2016.02.021

47. Bioinformatics, tissue distribution, and subcellular localization analyses of fk506 binding protein 12b from silkworms

Author:: Si Li; JingJing Fei; DanDan Cheng; Yongfeng Jin; Wenping Zhang; Yaozhou Zhang; Zhengbing Lv
Source:: Archives of insect biochemistry and physiology 2016 v.91 no.2 pp. 109-123
ISSN:: 0739-4462
Subject:: silk; silkworms; bioinformatics; Western blotting; receptors; digestive system; adults; polyclonal antibodies; tissue distribution; gene expression; reverse transcription; cytoplasm; nuclear membrane; introns; Escherichia coli; complementary DNA; chromosomes; polymerase chain reaction; silk glands; exons; Bombyx mori
Abstract:: ... FK506 binding proteins (FKBPs) are intracellular receptors of the immunosuppressant FK506 and play important roles in the correct folding of new proteins and the self‐assembly of biological macromolecules. FKBP12 is a member of the FKBP family that is widely expressed and highly conserved in many species. In this study, we identified the complete cDNA sequence encoding the FKBP12 ortholog in Bomby ...
DOI:: 10.1002/arch.21312
: https://doi.org/10.1002/arch.21312

48. Bisphenol A and dibutyl phthalate affect the expression of juxtacrine signaling factors in rat testis

Author:: Alicja Kamińska; Laura Pardyak; Sylwia Marek; Ewelina Górowska-Wójtowicz; Małgorzata Kotula-Balak; Barbara Bilińska; Anna Hejmej
Source:: Chemosphere 2018 v.199 pp. 182-190
ISSN:: 0045-6535
Subject:: Western blotting; adults; bisphenol A; dibutyl phthalate; explants; immunohistochemistry; ligands; males; messenger RNA; models; quantitative polymerase chain reaction; rats; reverse transcriptase polymerase chain reaction; seminiferous epithelium; signal transduction
Abstract:: ... The study was designed to examine the effects of model plastic derived compounds, bisphenol A (BPA) and dibutyl phthalate (DBP), on juxtacrine communication in adult rat testis, by evaluating the expression of Notch pathway components. Testicular explant were exposed in vitro to BPA (5 × 10⁻⁶ M, 2.5 × 10⁻⁵ M, 5 × 10⁻⁵ M) or DBP (10⁻⁶ M, 10⁻⁵ M, 10⁻⁴ M) for 24 h. To determine the expression of Notc ...
DOI:: 10.1016/j.chemosphere.2018.02.011
: https://doi.org/10.1016/j.chemosphere.2018.02.011

49. Bisphenol A induces spermatocyte apoptosis in rare minnow Gobiocypris rarus

Author:: Yingying Zhang; Mengqian Cheng; Lang Wu; Guo Zhang; Zaizhao Wang
Source:: Aquatic toxicology 2016 v.179 pp. 18-26
ISSN:: 0166-445X
Subject:: Gobiocypris rarus; Western blotting; adults; apoptosis; bisphenol A; endocrine-disrupting chemicals; gene expression; genes; males; mammals; meiosis; messenger RNA; minnows; mitochondria; pro-apoptotic proteins; quantitative polymerase chain reaction; spermatocytes; testes; transmission electron microscopes; transmission electron microscopy
Abstract:: ... Bisphenol A (BPA) is an endocrine disruptor, and could induce germ cells apoptosis in the testis of mammals. But whether it could affect fish in the same mechanism has not⿿ been studied till now. In the present study, to investigate the influence of BPA on testis germ cells in fish, adult male rare minnow Gobiocypris rarus were exposed to 225μgL⿿1 (0.99μM) BPA for 1, 3 and 9 weeks. Through TdT-med ...
DOI:: 10.1016/j.aquatox.2016.08.013
: https://doi.org/10.1016/j.aquatox.2016.08.013

50. CCDC134, a novel secretory protein, inhibits activation of ERK and JNK, but not p38 MAPK

Author:: Huang, J.; Shi, T.; Ma, T.; Zhang, Y.; Ma, X.; Lu, Y.; Song, Q.; Liu, W.; Ma, D.; Qiu, X.
Source:: Cellular and molecular life sciences 2008 v.65 no.2 pp. 338-349
ISSN:: 1420-682X
Subject:: Northern blotting; Western blotting; adults; amino acids; genes; humans; immunohistochemistry; messenger RNA; mitogen-activated protein kinase; nucleotides; phosphorylation; reverse transcriptase polymerase chain reaction; small interfering RNA; tissues; transcription (genetics)
Abstract:: ... In this study, we report a novel gene, CCDC134 (coiled-coil domain containing 134), that encodes a secretory protein that can inhibit the MAPK pathway as a novel human MAPK-regulating protein. The CCDC134 mRNA contains 1280 nucleotides, encoding a protein of 229 amino acids. CCDC134 is a classical secretory protein. Expression profile analysis by Northern blot, RT-PCR, immunohistochemistry and Wes ...
DOI:: 10.1007/s00018-007-7448-5
: https://doi.org/10.1007/s00018-007-7448-5

51. Can the activation of plasminogen/plasmin system of the host by metabolic products of Dirofilaria immitis participate in heartworm disease endarteritis?

Author:: Javier González-Miguel; Rodrigo Morchón; Elena Carretón; José Alberto Montoya-Alonso; Fernando Simón
Source:: Parasites & vectors 2015 v.8 no.1 pp. 194
ISSN:: 1756-3305
Subject:: Dirofilaria immitis; Western blotting; adults; antigens; cats; cell movement; cell proliferation; collagen; dogs; endothelial cells; extracellular matrix; fibrinolysis; gelatinase A; gentian violet; heartworms; models; myocytes; parasitoses; plasmin; plasminogen; smooth muscle; tissue repair
Abstract:: ... BACKGROUND: Proliferative endarteritis is one of the key pathological mechanisms of cardiopulmonary dirofilariosis, a cosmopolitan parasitosis caused by Dirofilaria immitis affecting dogs and cats around the world. It has been shown that the excretory/secretory antigens from D. immitis adult worms (DiES) bind plasminogen (PLG) and activate fibrinolysis, which can lead to a survival mechanism for t ...
DOI:: 10.1186/s13071-015-0799-0
PubMed:: 25888952
PubMed Central:: PMC4391138
: https://doi.org/10.1186/s13071-015-0799-0

52. Capsaicin protects against testicular torsion injury through mTOR-dependent mechanism

Author:: Nasim Javdan; Seyed Abdulmajid Ayatollahi; Muhammad Iqbal Choudhary; Safaa Al-Hasani; Farzad Kobarfard; Ata Athar; Hamidreza Pazoki-Toroudi
Source:: Theriogenology 2018 v.113 pp. 247-252
ISSN:: 0093-691X
Subject:: Western blotting; adults; analgesia; antioxidant activity; apoptosis; capsaicin; neoplasms; oxidative stress; peppers; phosphorylation; rapamycin; rats; signal transduction; testes
Abstract:: ... Testicular torsion and subsequent release of reactive oxygen species (ROS) can cause infertility in adults. Oxidative stress following testicular torsion plays an important role in the ýonset and development of apoptotic cell death through dysregulation of the cellular signaling pathways. Anti-inflammatory and antioxidant properties of capsaicin, a bioactive composition present in red peppers, has ...
DOI:: 10.1016/j.theriogenology.2018.03.012
: https://doi.org/10.1016/j.theriogenology.2018.03.012

53. Caracterization of glutathione S-transferase of Dermacantor marginatus and effect of the recombinant antigen as a potential anti-tick vaccine

Author:: Huercha; Ruiqi Song; Min Li; Xinli Fan; Zhengxiang Hu; Lijiang Wu; Yongchang Li; Wei Zhang; Yang Zhang; Yuhui Ma; Chahan Bayin
Source:: Veterinary parasitology 2020 v.279 pp. 109043
ISSN:: 0304-4017
Subject:: Dermacentor marginatus; Dermacentor variabilis; Western blotting; acaricide resistance; acaricides; adults; affinity chromatography; amino acid sequences; amino acids; antigens; control methods; eggs; engorgement; environmental assessment; enzyme activity; females; genes; glutathione transferase; hatching; hematophagy; livestock; nucleotide sequences; pathogens; polyclonal antibodies; rabbits; recombinant proteins; tick control; tick infestations; ticks; transcription (genetics); vaccination; vaccines; China
Abstract:: ... Dermacentor marginatus is one of the main tick species in northwestern China, and is a vector of various tick-borne pathogens. Tick control method largely depends on chemical agents, but the disadvantages of using such approach would cause environmental damage and the risk of developing tick resistance to acaricides. Vaccination of tick protective antigen is an eco-friendly approach which is an al ...
DOI:: 10.1016/j.vetpar.2020.109043
: https://doi.org/10.1016/j.vetpar.2020.109043

54. Cellular Distribution of Anionic Antimicrobial Peptide in Normal Lung and during Acute Pulmonary Inflammation

Author:: A. J. Fales-Williams; K. A. Brogden; E. Huffman; J. M. Gallup; M. R. Ackermann
Source:: Veterinary pathology v.39 no.6 pp. 706-711
ISSN:: 1544-2217
Subject:: Mannheimia; Pasteurella; Pseudomonas aeruginosa; Western blotting; adults; animal pathology; antibodies; antimicrobial peptides; bacteria; bronchioles; calves; cows; humans; immunohistochemistry; inflammation; inoculum; lipopolysaccharides; respiratory tract diseases; sheep
Abstract:: ... Anionic peptides (APs) are small antimicrobial peptides present in human and ovine lung. In this study APs were also detected in bovine lung, and production of APs in lungs with acute inflammation induced by various stimuli was determined. The distribution and intensity of APs were determined by immunohistochemistry in lungs of 1) neonatal calves (1-3 days of age) inoculated with Mannheimia (Paste ...
DOI:: 10.1354/vp.39-6-706
: https://doi.org/10.1354/vp.39-6-706

55. Characterisation of major vault protein during the life cycle of the human parasite Schistosoma mansoni

Author:: Eneida V. Reis; Roberta V. Pereira; Matheus Gomes; Liana K. Jannotti-Passos; Elio H. Baba; Paulo Marcos Zech Coelho; Ana C.A. Mattos; Flávia F.B. Couto; William Castro-Borges; Renata Guerra-Sá
Source:: Parasitology international 2014 v.63 no.1 pp. 120-126
ISSN:: 1383-5769
Subject:: Caenorhabditis elegans; Drosophila melanogaster; Schistosoma mansoni; Western blotting; adults; bioinformatics; cercariae; eukaryotic cells; females; gene expression regulation; genes; genomics; humans; males; models; multiple drug resistance; non-coding RNA; parasites; phenotype; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; ribonucleoproteins; schistosomula; signal transduction
Abstract:: ... Vaults are ribonucleoproteins (13 MDa) highly conserved among lower and higher eukaryotes. Their association produces a complex composed of three proteins named Major Vault Protein (MVP), vault (PolyADP-ribose) polymerase (VPARP) and Telomerase-associated protein (TEP1), plus a small untranslated RNA. The exact function of this complex is unknown, although the biological role of vaults has been as ...
DOI:: 10.1016/j.parint.2013.10.005
: https://doi.org/10.1016/j.parint.2013.10.005

56. Characteristics and function of cathepsin L3 from Schistosoma japonicum

Author:: Wenling Huang; Mengjie Gu; Wenjun Cheng; Qin Ping Zhao; Zhenping Ming; Huifen Dong
Source:: Parasitology research 2020 v.119 no.5 pp. 1619-1628
ISSN:: 0932-0113
Subject:: Schistosoma japonicum; Western blotting; adjuvants; adults; animal diseases; cathepsins; cytotoxicity; digestive tract; egg shell; eggs; fluorescent antibody technique; immunization; liver; livestock; macrophages; mice; quantitative polymerase chain reaction; schistosomiasis; schistosomula; vaccine development; vaccines
Abstract:: ... Schistosomiasis is still prevalent and seriously endangering the health of people and livestock in many countries. There have been great efforts to develop vaccines against schistosomiasis for prolonged protection in epidemic areas. Molecules from lung-stage schistosomula have been regarded as potential vaccine candidates against schistosomiasis. Our previous work has shown that cathepsin L3 from ...
DOI:: 10.1007/s00436-020-06647-x
: https://doi.org/10.1007/s00436-020-06647-x

57. Characterization and dietary regulation of oligopeptide transporter (PepT1) in different ploidy fishes

Author:: Zhen Liu; Yi Zhou; Shaojun Liu; Qiong Zhao; Junchang Feng; Shuangqing Lu; Gang Xiong; Dizhi Xie; Jianshe Zhang; Yun Liu
Source:: Peptides 2014 v.52 pp. 149-156
ISSN:: 0196-9781
Subject:: Carassius; Western blotting; absorption; additives; adults; breeding season; complementary DNA; diploidy; eggs; embryogenesis; fish; fish meal; gene expression; genes; intestinal mucosa; messenger RNA; microvilli; muscle contraction; peptide transporters; protein content; protein sources; quantitative polymerase chain reaction; sequence homology; sodium butyrate; soybean meal; tetraploidy; triploidy
Abstract:: ... The oligopeptide transporter (PepT1) is located on the brush-border membrane of the intestinal epithelium which has been regarded as a mediator of protein absorption. Here, we cloned and characterized PepT1 genes from diploid (red crucian carp), triploid and tetraploid fish. Then, the PepT1 expression pattern in different tissues and embryogenesis were assayed. Meanwhile, using real-time PCR and w ...
DOI:: 10.1016/j.peptides.2013.12.017
: https://doi.org/10.1016/j.peptides.2013.12.017

58. Characterization and expression analysis of an intelectin gene from Megalobrama amblycephala with excellent bacterial binding and agglutination activity

Author:: Zhujin Ding; Xiaoheng Zhao; Qifeng Zhan; Lei Cui; Qianhui Sun; Li Lin; Weimin Wang; Hong Liu
Source:: Fish & shellfish immunology 2017 v.61 pp. 100-110
ISSN:: 1050-4648
Subject:: Aeromonas hydrophila; Escherichia coli; Megalobrama amblycephala; Western blotting; adults; agglutination; amphibians; complementary DNA; cysteine; embryogenesis; fish; gene expression regulation; genes; gills; hatching; immunohistochemistry; innate immunity; intestines; iron absorption; kidneys; lectins; liver; mammals; messenger RNA; sequence analysis; shellfish; spleen; tissues
Abstract:: ... Intelectin is a recently discovered lectin that plays vital roles in the innate immune response, iron metabolism and early embryogenesis. The structure, expression pattern and function of intelectin in mammals and amphibians have been well studied, while not well known in fish. In this study, we cloned a intelectin (MamINTL) gene from blunt snout bream (Megalobrama amblycephala), examined its expr ...
DOI:: 10.1016/j.fsi.2016.12.023
: https://doi.org/10.1016/j.fsi.2016.12.023

59. Characterization and expression of cathepsin B2 in Fasciola gigantica

Author:: Pathanin Chantree; Chaitip Wanichanon; Manussabhorn Phatsara; Krai Meemon; Prasert Sobhon
Source:: Experimental parasitology 2012 v.132 no.2 pp. 249-256
ISSN:: 0014-4894
Subject:: Komagataella pastoris; Western blotting; digestion; parasitology; gelatin; epithelium; flukes; adults; juveniles; cross reaction; immunoglobulin G; metacercariae; cathepsin B; antiserum; fluorometry; antigens; Fasciola gigantica; collagen; connective tissues; mice; immune system; enzyme activity; fascioliasis; protein synthesis; vaccines
Abstract:: ... Fasciola gigantica cathepsin B belongs to a family of cysteine proteases which is involved in invasion of host tissues. In this study, the recombinant cathepsin B2 (rFgCatB2), synthesized in Pichia pastoris, showed enzymatic activity on a fluorometric substrate Z-Phe-Arg-AMC and gelatin. Furthermore, this recombinant enzyme could degrade IgG and type I collagen. Mouse antiserum against rFgCatB2 re ...
DOI:: 10.1016/j.exppara.2012.07.011
: https://doi.org/10.1016/j.exppara.2012.07.011

60. Characterization and localization of antigens for serodiagnosis of human paragonimiasis

Author:: Kurt C. Curtis; Kerstin Fischer; Young-Jun Choi; Makedonka Mitreva; Gary J. Weil; Peter U. Fischer
Source:: Parasitology research 2021 v.120 no.2 pp. 535-545
ISSN:: 0932-0113
Subject:: Escherichia coli; Paragonimus heterotremus; Paragonimus kellicotti; Paragonimus miyazakii; Paragonimus westermani; Philippines; Western blotting; adults; amino acids; antigens; cough; cysteine proteinases; egg yolk; eggs; fascioliasis; ferritin; fever; genome; humans; lung flukes; lung neoplasms; myoglobin; paragonimiasis; parasitology; patients; praziquantel; research; schistosomiasis; serodiagnosis; transcription (genetics); tuberculosis
Abstract:: ... Paragonimiasis is a foodborne trematode infection that affects 23 million people, mainly in Asia. Lung fluke infections lead frequently to chronic cough with fever and hemoptysis, and are often confused with lung cancer or tuberculosis. Paragonimiasis can be efficiently treated with praziquantel, but diagnosis is often delayed, and patients are frequently treated for other conditions. To improve d ...
DOI:: 10.1007/s00436-020-06990-z
: https://doi.org/10.1007/s00436-020-06990-z

61. Characterization of Melanoplus sanguinipes oviposition stimulating protein expression and re-examination of its potential role in stimulating oviposition

Author:: E.A. Sieminska; J.A. Andres; C.D. Todd; M.A. Erlandson
Source:: Journal of insect physiology 2015 v.73 pp. 37-46
ISSN:: 0022-1910
Subject:: Melanoplus sanguinipes; Western blotting; adults; aedeagus; amino acid sequences; antibodies; copulation; grasshoppers; insect physiology; males; mechanism of action; messenger RNA; open reading frames; oviducts; oviposition; precipitin tests; protein synthesis; sequence homology; spermatheca; spermatophores; tissues; virgin females
Abstract:: ... Melanoplus sanguinipes oviposition stimulating protein (MsOSP) was characterized and its role in stimulating oviposition in virgin females was examined. A 967nt MsOSP mRNA sequence with homology to previously characterized N-terminal amino acid sequence data for MsOSP was identified in a RNAseq library generated from an mRNA pool from the long hyaline tubule (LHT) of the male accessory gland compl ...
DOI:: 10.1016/j.jinsphys.2015.01.003
: https://doi.org/10.1016/j.jinsphys.2015.01.003

62. Characterization of Ostertagia ostertagi annexin-like proteins at different developmental stages

Author:: Pooja Sharma; Mark Jenkins; Dante Zarlenga; Ray Fetterer; Zhengguo Xiao; Wenbin Tuo
Source:: Parasitology research 2017 v.116 no.5 pp. 1515-1522
ISSN:: 0932-0113
Subject:: Ostertagia ostertagi; Western blotting; abomasum; absorbance; adults; amino acid sequences; antibodies; calves; eukaryotic cells; gastric mucosa; host-parasite relationships; immune response; larvae; leukocytes; parasites; receptors; staining; temperate zones; vaccines
Abstract:: ... Ostertagiosis remains an economically important parasitic disease in cattle in the temperate regions of the world. Repeated exposures to Ostertagia ostertagi in calves cause significant pathology in the abomasum but elicit little protective immunity. The larvae use the host’s gastric glands as a niche for development, where the parasite completes its parasitic stages, while in the gastric glands, ...
Handle:: 10113/5726150
DOI:: 10.1007/s00436-017-5428-8
CHORUS:: 10.1007/s00436-017-5428-8
: https://doi.org/10.1007/s00436-017-5428-8

63. Characterization of TsDAF-21/HSP90 protein from the parasitic nematode Trichinella spiralis

Author:: Yurong Yang; Weiwen Qin; Hengtong Qiu; Yan Liu
Source:: Parasitology research 2014 v.113 no.6 pp. 2209-2217
ISSN:: 0932-0113
Subject:: Escherichia coli; Trichinella spiralis; Western blotting; adults; ampicillin; antigens; blood serum; cold stress; complementary DNA; gentamicin; heat stress; mice; muscle larvae; neonates; temperature; trichinosis
Abstract:: ... The Tsdaf-21 cDNA was cloned into pET-32a and subsequently expressed in Escherichia coli. The recombinant protein TsDAF-21 was purified and evaluated as an antigen in Western blot. The serum from mouse 14, 21, and 28 days after being infected with Trichinella spiralis recognized rTsDAF-21, but the serum from mouse 7 days post infection of T. spiralis could not react with rTsDAF-21. It implied that ...
DOI:: 10.1007/s00436-014-3874-0
PubMed:: 24710664
: https://doi.org/10.1007/s00436-014-3874-0

64. Characterization of TsMRP-L28, a mitochondrial ribosomal protein L28 from the parasitic nematode Trichinella spiralis

Author:: Yurong Yang; Wei Jian; Weiwen Qin; Guimei Tian
Source:: Gene 2013 v.512 pp. 496-504
ISSN:: 0378-1119
Subject:: Caenorhabditis elegans; Trichinella spiralis; Western blotting; adults; amino acids; complementary DNA; cytoplasm; early development; muscle larvae; neonates; phylogeny; protein synthesis
Abstract:: ... A cDNA encoding mitochondrial ribosomal protein (MRP)-L28 was isolated and cloned from Trichinella spiralis, an economically important parasitic nematode. The predicted TsMRP-L28 protein consists of 276 amino acids. Phylogenetic analysis revealed that TsMRP-L28 was closely related to Caenorhabditis elegans mitochondrial ribosomal protein L28. The TsMRP-L28 transcript was expressed in newborn larva ...
DOI:: 10.1016/j.gene.2012.10.010
PubMed:: 23089495
: https://doi.org/10.1016/j.gene.2012.10.010

65. Characterization of a Trichinella spiralis 31kDa protein and its potential application for the serodiagnosis of trichinellosis

Author:: Jing Cui; Li Wang; Ge Ge Sun; Li Na Liu; Shuai Bing Zhang; Ruo Dan Liu; Xi Zhang; Peng Jiang; Zhong Quan Wang
Source:: Acta tropica 2015 v.142 pp. 57-63
ISSN:: 0001-706X
Subject:: Escherichia coli; Trichinella spiralis; Western blotting; adults; antibodies; antigens; blood serum; enzyme-linked immunosorbent assay; genes; humans; immunoglobulin G; mice; molecular cloning; muscle larvae; neonates; parasites; patients; serodiagnosis; trichinosis
Abstract:: ... The Trichinella spiralis 31kDa protein (Ts31) was screened from the excretory-secretory (ES) proteins of muscle larvae (ML) by immunoproteomics using serum from mice infected with T. spiralis at 18 days post infection (dpi). The aim of this study was to characterize the Ts31 protein and to evaluate the potential of the recombinant Ts31 protein (rTs31) for serodiagnosis of human trichinellosis. Ts3 ...
DOI:: 10.1016/j.actatropica.2014.10.017
: https://doi.org/10.1016/j.actatropica.2014.10.017

66. Characterization of a Trichinella spiralis cathepsin X and its promotion for the larval invasion of mouse intestinal epithelial cells

Author:: Shu Wei Yan; Yuan Yuan Hu; Yan Yan Song; Hua Nan Ren; Jia Ming Shen; Ruo Dan Liu; Shao Rong Long; Peng Jiang; Jing Cui; Zhong Quan Wang
Source:: Veterinary parasitology 2021 v.297 pp. 109160
ISSN:: 0304-4017
Subject:: Escherichia coli; Trichinella spiralis; Western blotting; adults; antibodies; confocal microscopy; cytoplasm; mice; muscles; neonates; nucleotide sequences; veterinary parasitology
Abstract:: ... The aim of this study was to ascertain the characteristics of a Trichinella spiralis cathepsin X (TsCX) and its role on larval invasion of intestinal epithelial cells (IECs). The full-length of TsCX cDNA sequence was cloned and expressed in Escherichia coli BL21. The results of RT-PCR, IFA and Western blot revealed that TsCX was expressed at T. spiralis muscle larvae (ML), intestinal infective lar ...
DOI:: 10.1016/j.vetpar.2020.109160
: https://doi.org/10.1016/j.vetpar.2020.109160

67. Characterization of a chymotrypsin-like enzyme from Trichinella spiralis and its facilitation of larva penetration into the host's enteral epithelial cells

Author:: Jia Xu; Ruo Dan Liu; Shao Rong Long; Yan Yan Song; Peng Jiang; Xi Zhang; Jing Cui; Zhong Quan Wang
Source:: Research in veterinary science 2020 v.128 pp. 1-8
ISSN:: 0034-5288
Subject:: Escherichia coli; Trichinella spiralis; Western blotting; adults; antibodies; complementary DNA; confocal microscopy; cytoplasm; enzyme-linked immunosorbent assay; enzymes; epithelial cells; epithelium; intestines; muscle larvae; nucleotide sequences; parasites; proteins; reverse transcriptase polymerase chain reaction; vaccines
Abstract:: ... The aim of this work was to identify the molecular characteristics of a chymotrypsin-like enzyme from Trichinella spiralis (Tschy) and its facilitation of larval penetration into enteral epithelial cells (EECs). The complete Tschy cDNA sequence was cloned and expressed in Escherichia coli BL21. RT-PCR, IIFA and western blotting showed that Tschy was expressed at the T. spiralis muscle larvae (ML), ...
DOI:: 10.1016/j.rvsc.2019.10.018
: https://doi.org/10.1016/j.rvsc.2019.10.018

68. Characterization of a novel aspartyl protease inhibitor from Haemonchus contortus

Author:: Baojie Li; Javaid Ali Gadahi; Wenxiang Gao; Zhenchao Zhang; Muhammad Ehsan; Lixin Xu; Xiaokai Song; Xiangrui Li; Ruofeng Yan
Source:: Parasites & vectors 2017 v.10 no.1 pp. 191
ISSN:: 1756-3305
Subject:: Ascaris suum; Brugia malayi; DNA fragmentation; Haemonchus contortus; Ostertagia ostertagi; Western blotting; adults; amino acid sequences; blood serum; enzyme inhibition; epithelial cells; females; gastrointestinal system; gene expression regulation; genes; goats; immune response; in vitro studies; interferon-gamma; interleukin-10; interleukin-4; larvae; molecular weight; mononuclear leukocytes; nematode infections; pH; pepsin; proteinase inhibitors; proteins; proteolysis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; temperature
Abstract:: ... BACKGROUND: Aspartyl protease inhibitor (API) was thought to protect intestinal parasitic nematodes from their hostile proteolytic environment. Studies on Ostertagia ostertagi, Ascaris suum and Brugia malayi indicated that aspins might play roles in nematode infection. In a recent study, proteins differentially expressed between free-living third-stage larvae (L3) and activated L3 (xL3) of Haemonc ...
DOI:: 10.1186/s13071-017-2137-1
PubMed:: 28420411
PubMed Central:: PMC5395858
: https://doi.org/10.1186/s13071-017-2137-1

69. Characterization of a piRNA binding protein Miwi in mouse oocytes

Author:: Xiaofang Ding; Huangtao Guan; Honggang Li
Source:: Theriogenology 2013 v.79 no.4 pp. 610-615.e1
ISSN:: 0093-691X
Subject:: Western blotting; adults; animal ovaries; binding proteins; cytoplasmic granules; females; fluorescent antibody technique; follicular development; messenger RNA; mice; microRNA; oocytes; quantum dots; reverse transcriptase polymerase chain reaction; small interfering RNA; spermatogenesis; stem cells
Abstract:: ... Argonaute proteins and Piwi proteins bind with microRNA (mRNA) and Piwi-interacting RNA (piRNA), respectively, to form functional complexes. Piwi proteins are mostly restricted to germ cells and stem cells, and the Piwi-piRNA pathway is required for normal spermatogenesis. Although piRNAs were also recently identified in mammalian oocytes, expression of Piwi proteins in the ovary has not been well ...
DOI:: 10.1016/j.theriogenology.2012.11.013
PubMed:: 23244769
: https://doi.org/10.1016/j.theriogenology.2012.11.013

70. Characterization of a secreted cystatin from the tick Rhipicephalus haemaphysaloides

Author:: Yujian Wang; Xinmao Yu; Jie Cao; Yongzhi Zhou; Haiyan Gong; Houshuang Zhang; Xiangrui Li; Jinlin Zhou
Source:: Experimental & applied acarology 2015 v.67 no.2 pp. 289-298
ISSN:: 0168-8162
Subject:: Escherichia coli; Rhipicephalus; Western blotting; acarology; adults; amino acids; cathepsin L; complementary DNA; cystatins; eggs; enzyme activity; genes; glutathione; isoelectric point; midgut; molecular weight; open reading frames; papain; recombinant proteins; reverse transcriptase polymerase chain reaction; saliva; sequence analysis; signal peptide; ticks
Abstract:: ... A novel cystatin, designated RHcyst-2, was isolated from the tick Rhipicephalus haemaphysaloides. The full-length cDNA of RHcyst-2 is 773 bp, including an intact open reading frame encoding an expected protein of 139 amino acids and consisting of a 23 amino acids signal peptide. Predicted RHcyst-2 mature protein molecular weight is about 13 kDa, isoelectric point is 4.96. A sequence analysis showe ...
DOI:: 10.1007/s10493-015-9946-8
: https://doi.org/10.1007/s10493-015-9946-8

71. Characterization of an MLP Homologue from Haemaphysalis longicornis (Acari: Ixodidae) Ticks

Author:: Jin Luo; Hui Shen; Qiaoyun Ren; Guiquan Guan; Bo Zhao; Hong Yin; Ronggui Chen; Hongying Zhao; Jianxun Luo; Xiangrui Li; Guangyuan Liu
Source:: Pathogens 2020 v.9 no.4 pp. -
ISSN:: 2076-0817
Subject:: Escherichia coli; Haemaphysalis longicornis; Western blotting; adults; antiserum; engorgement; gene expression; genes; immunity; information; integument; lead; mortality; muscle development; muscles; myocytes; pathogens; quantitative polymerase chain reaction; rabbits; rapid amplification of cDNA ends; thermal stress; ticks; tissues; transcription (genetics); vaccination; vaccines; weight
Abstract:: ... Members of the cysteine-rich protein (CRP) family are known to participate in muscle development in vertebrates. Muscle LIM protein (MLP) belongs to the CRP family and has an important function in the differentiation and proliferation of muscle cells. In this study, the full-length cDNA encoding MLP from Haemaphysalis longicornis (H. longicornis; HLMLP) ticks was obtained by 5′ ...
DOI:: 10.3390/pathogens9040284
: https://doi.org/10.3390/pathogens9040284

72. Characterization of an antigenic serine protease in the Trichinella spiralis adult

Author:: He Gao; Bin Tang; Xue Bai; Libo Wang; Xiuping Wu; Haining Shi; Xuelin Wang; Xiaolei Liu; Mingyuan Liu
Source:: Experimental parasitology 2018 v.195 pp. 8-18
ISSN:: 0014-4894
Subject:: Escherichia coli; Trichinella spiralis; Western blotting; adults; blood coagulation; cDNA libraries; cell nucleolus; coagulation; complementary DNA; enzyme activity; fluorescence; genes; helminthiasis; hosts; muscle larvae; muscles; neonates; parasites; plasminogen; serine; serine proteinases
Abstract:: ... Serine proteases have been identified as important molecules that are involved in many parasitic infections, and these molecules have also been suggested to play important roles in Trichinella spiralis infections. In the present study, the antigenic serine protease gene Ts-ADSp-7, which was screened from a cDNA library of Trichinella spiralis Adults at 3 days post-infection (p.i.), was cloned and ...
DOI:: 10.1016/j.exppara.2018.09.009
: https://doi.org/10.1016/j.exppara.2018.09.009

73. Characterization of cathepsin B proteinase (AcCP-2) in eggs and larvae stages of hookworm Ancylostoma caninum

Author:: Yurong Yang; Weiwen Qin; Hua Wei; Jianxi Ying; Jing Zhen
Source:: Experimental parasitology 2011 v.129 no.3 pp. 215-220
ISSN:: 0014-4894
Subject:: Ancylostoma caninum; Escherichia coli; Western blotting; adults; cathepsin B; complementary DNA; digestion; early development; eggs; enzyme activity; gelatin; hookworms; larvae; parasitology
Abstract:: ... Cathepsin B proteinase constitutes a large multigenes family in parasitic and non-parasitic nematodes. The localization of cathepsin B proteinases (AcCP-1 and AcCP-2) in adult worm of Ancylostoma caninum has been characterized (Harrop et al., 1995), but the localization and function in eggs and larval stages remained undiscovered. Here we described the expressing of cathepsin B proteinase (AcCP-2) ...
DOI:: 10.1016/j.exppara.2011.08.018
: https://doi.org/10.1016/j.exppara.2011.08.018

74. Characterization of excretory-secretory antigens of adult Toxocara canis by western blotting

Author:: N. R. Sudhakar; S. Samanta; Shivani Sahu; O. K. Raina; S. C. Gupta; T. K. Goswami; K. M. Lokesh; Ashok Kumar
Source:: Journal of parasitic diseases 2014 v.38 no.2 pp. 166-169
ISSN:: 0971-7196
Subject:: Toxocara canis; Western blotting; adults; ammonium sulfate; antigens; antiserum; blood serum; children; diagnostic techniques; dogs; helminths; humans; polyacrylamide gel electrophoresis; polypeptides; proteins; public health; rabbits; sodium; toxocariasis
Abstract:: ... Toxocara canis is one of the most common helminth worm of dogs which continues to stimulate both public health concern alongside the higher scientific interest. It may cause visceral and ocular damage in humans especially in children. The identification of specific antigens of T. canis is important so as to develop better diagnostic techniques. Excretory-secretory (ES) antigens were prepared by cu ...
DOI:: 10.1007/s12639-012-0209-x
PubMed:: 24808645
PubMed Central:: PMC4000380 [Available on 06/01/15]
: https://doi.org/10.1007/s12639-012-0209-x

75. Characterization of four urotensin II receptors (UTS2Rs) in chickens

Author:: Lin Cui; Can Lv; Jiannan Zhang; Juan Li; Yajun Wang
Source:: Peptides 2021 v.138 pp. 170482
ISSN:: 0196-9781
Subject:: Western blotting; adults; amino acid sequences; brain; calcium; chickens; genes; humans; luciferase; peptides; quantitative polymerase chain reaction; sequence analysis; spinal cord; spleen
Abstract:: ... Urotensin II receptor (UTS2R) is suggested to mediate the actions of urotensin II (UTS2) and UTS2-related peptide (URP, also called UTS2B) in mammals. However, the information regarding the gene structure, functionality and tissue expression of UTS2/URP receptor remains largely unknown in non-mammalian vertebrates including birds. In this study, using RACE-PCR, we cloned the full-length cDNAs of f ...
DOI:: 10.1016/j.peptides.2020.170482
: https://doi.org/10.1016/j.peptides.2020.170482

76. Characterization of genes with a putative key role in the parasitic lifestyle of the nematode Strongyloides ratti

Author:: W. G. SPINNER; F. J. THOMPSON; D. C. EMERY; M. E. VINEY
Source:: Parasitology 2012 v.139 no.10 pp. 1317-1328
ISSN:: 1469-8161
Subject:: excretory-secretory products; parasitism; Strongyloides; humans; microarray technology; recombinant proteins; vaccines; models; expressed sequence tags; reverse transcriptase polymerase chain reaction; Western blotting; gastrointestinal nematodes; gene expression; pathogens; rats; people; adults
Abstract:: ... Parasitic nematodes are significant pathogens of humans and other animals. The molecular and genetic basis of animal parasitism is not yet fully understood. Strongyloides spp. are a genus of gastrointestinal nematodes of which species infect approximately 100–200 million people worldwide. S. ratti is a natural parasite of the rat, and a useful and amenable laboratory model. Previous EST and ...
DOI:: 10.1017/S0031182012000637
PubMed:: 22717055
: https://doi.org/10.1017/S0031182012000637

77. Characterization of phosphodiesterase-5 as a surface protein in the tegument of Schistosoma mansoni

Author:: Rofatto, Henrique K.; Tararam, Cibele A.; Borges, William C.; Wilson, R. Alan; Leite, Luciana C.C.; Farias, Leonardo P.
Source:: Molecular and biochemical parasitology 2009 v.166 no.1 pp. 32-41
ISSN:: 0166-6851
Subject:: Schistosoma mansoni; Western blotting; adults; antibodies; cercariae; developing countries; disease control; enzyme activity; gene expression regulation; hydrolysis; mammals; proteomics; reverse transcriptase polymerase chain reaction; schistosomiasis; signal peptide; surface proteins; vaccines
Abstract:: ... Schistosoma mansoni is a major causative agent of schistosomiasis, an important parasitic disease that constitutes a severe health problem in developing countries. Even though an effective treatment exists, it does not prevent re-infection and the development of an effective vaccine still remains the most desirable means of control for this disease. In this work we describe the cloning and charact ...
DOI:: 10.1016/j.molbiopara.2009.02.006
: https://doi.org/10.1016/j.molbiopara.2009.02.006

78. Characterization of primary structure and post-hatching increase in chicken cytosolic acetoacetyl-coA thiolase in the liver

Author:: N. Nakao; H. Kaneda; N. Tsushima; M. Tanaka
Source:: Poultry science 2016 v.95 no.6 pp. 1406-1410
ISSN:: 0032-5791
Subject:: Western blotting; acetyl coenzyme A; acetyl-CoA acetyltransferase; adrenal glands; adults; amino acid sequences; amino acids; cholesterol; diet; enzyme activity; humans; hydrolysis; jejunum; kidneys; liver; nucleotide sequences; protein synthesis; quantitative polymerase chain reaction; rats; tissue distribution
Abstract:: ... Acetoacetyl-CoA thiolase (EC 2.3.1.9) catalyzes the cleavage of acetoacetyl-CoA into acetyl-CoA and its reverse reaction, the synthesis of acetoacetyl-CoA. Cytosolic acetoacetyl-CoA thiolase (CT) is a key enzyme in the initial step of the cholesterol synthesis pathway. In the present study, we characterized the amino acid sequence of chicken CT and the tissue distribution of its mRNA and protein, ...
DOI:: 10.3382/ps/pew045
: https://doi.org/10.3382/ps/pew045

79. Characterization of red-spotted grouper nervous necrosis virus isolated from ovarian fluids of asymptomatic wild Asian seabass, Lates calcarifer

Author:: Venkata Satyanarayana Nallala; M. Makesh; K. Radhika; T. Sathish Kumar; P. Raja; R. Subburaj; M. Kailasam; K.K. Vijayan
Source:: Aquaculture 2021 v.542 pp. 736846
ISSN:: 0044-8486
Subject:: Lates calcarifer; Redspotted grouper nervous necrosis virus; Western blotting; adults; aquaculture; bioinformatics; brackish water; cell culture; cell lines; cesium; chlorides; coat proteins; freshwater; inactivated vaccines; juveniles; mortality; necrosis; polyacrylamide gel electrophoresis; ultracentrifugation; viral genome; virus replication; viruses
Abstract:: ... Viral nervous necrosis (VNN) caused by nervous necrosis virus is a serious disease affecting a wide range of marine, brackishwater and freshwater fishes. The disease causes acute mortalities in larval and early juvenile stages while adult fishes are asymptomatic and remain as carriers of the virus. In the present study, nervous necrosis virus was isolated from ovarian fluids of asymptomatic wild-c ...
DOI:: 10.1016/j.aquaculture.2021.736846
: https://doi.org/10.1016/j.aquaculture.2021.736846

80. Characterization of the equine blood–testis barrier during tubular development in normal and cryptorchid stallions

Author:: K. Rode; H. Sieme; P. Richterich; R. Brehm
Source:: Theriogenology 2015 v.84 no.5 pp. 763-772
ISSN:: 0093-691X
Subject:: Sertoli cells; Western blotting; adherens junctions; adults; apoptosis; cadherins; castration; cryptorchidism; cytoplasm; gap junctions; immunohistochemistry; men; mice; puberty; seminiferous tubules; spermatocytes; spermatogenesis; stallions; testicular development; tight junctions
Abstract:: ... The formation of the blood–testis barrier (BTB) is defined as occurring with the first appearance of spermatocytes at around puberty and is vital for normal spermatogenesis. This barrier between two adjacent Sertoli cells (SCs) consists of a cell junctional protein complex, which includes tight junctions (TJs), adherens junctions, and gap junctions. In many mammalian species, BTB composition has a ...
DOI:: 10.1016/j.theriogenology.2015.05.009
: https://doi.org/10.1016/j.theriogenology.2015.05.009

81. Characterization of the small hive beetle transcriptome focused on the insecticide target site and RNA interference genes

Author:: Kyungmun Kim; Sang Hyeon Kim; Kyungjae Andrew Yoon; Yun Sang Cho; Mi-Sun Yoo; Si Hyeock Lee
Source:: Journal of Asia-Pacific entomology 2018 v.21 no.4 pp. 1256-1261
ISSN:: 1226-8615
Subject:: Aethina tumida; Leptinotarsa decemlineata; RNA interference; Western blotting; acetylcholinesterase; adults; apiaries; carbamate pesticides; control methods; electrophoresis; genes; honey bee colonies; insecticides; larvae; mutation; overwintering; pests; polyacrylamide; pyrethrins; sodium channels; transcriptome; Korean Peninsula
Abstract:: ... The small hive beetle (SHB), Aethina tumida, is an invasive pest species in most Northern Hemisphere countries, including Korea. SHB causes serious damage to apiaries by destroying overwintering honey bee colonies. To obtain basic information for efficient management of SHB, genes encoding conventional insecticide targets, specifically the voltage-sensitive sodium channel α-subunit (VSSC) and acet ...
DOI:: 10.1016/j.aspen.2018.09.013
: https://doi.org/10.1016/j.aspen.2018.09.013

82. Characterization of vasa in the gonads of different ploidy fish

Author:: Fan Yu; Huan Zhong; Gang Liu; Shaojun Liu; Zhuohui Zhang; Yi Zhou; Min Tao; Yun Liu
Source:: Gene 2015 v.574 pp. 337-344
ISSN:: 0378-1119
Subject:: Western blotting; adults; breeding season; diploidy; embryogenesis; fish; genes; germ cells; immunohistochemistry; messenger RNA; testes; tetraploidy; triploidy
Abstract:: ... Vasa is an essential gene for germ cell development belonging to the DEAD-box family. In this study, we comparatively analyzed the expression characteristics of vasa in diploids, triploids, and tetraploids. The sequences showed high similarity among these fish and other vertebrates, with characteristic domains. Tissue expression analysis revealed that vasa was expressed exclusively in the gonad of ...
DOI:: 10.1016/j.gene.2015.08.016
: https://doi.org/10.1016/j.gene.2015.08.016

83. Characterizing the relative abundance of circadian transcription factors in diapausing and nondiapausing Northern house mosquitoes

Author:: Caitlin Peffers; Megan E. Meuti
Source:: Journal of insect physiology 2022 pp. 104404
ISSN:: 0022-1910
Subject:: Culex pipiens; West Nile virus; adults; autumn; diapause; insect physiology; photoperiod; summer
Abstract:: ... The Northern house mosquito (Culex pipiens) is a major vector of West Nile virus. To survive harsh conditions in winter adult females of Cx. pipiens enter a state of arrested reproductive development called diapause. Diapause is triggered by the short daylengths of late summer and early fall. The methods by which Cx. pipiens measures daylength are still unknown. However, it is suspected that clock ...
DOI:: 10.1016/j.jinsphys.2022.104404
: https://doi.org/10.1016/j.jinsphys.2022.104404

84. Chicken GRIFIN: A homodimeric member of the galectin network with canonical properties and a unique expression profile

Author:: Gabriel García Caballero; Herbert Kaltner; Malwina Michalak; Nadezhda Shilova; Michelle Yegres; Sabine André; Anna-Kristin Ludwig; Joachim C. Manning; Sebastian Schmidt; Martina Schnölzer; Nicolai V. Bovin; Dietmar Reusch; Jürgen Kopitz; Hans-Joachim Gabius
Source:: Biochimie 2016 v.128-129 pp. 34-47
ISSN:: 0300-9084
Subject:: Western blotting; adults; agglutinins; chickens; genes; humans; immunohistochemistry; lactose; mass spectrometry; microarray technology; peptides; rats; reverse transcriptase polymerase chain reaction
Abstract:: ... Occurrence of the adhesion/growth-regulatory galectins as family sets the challenge to achieve a complete network analysis. Along this route taken for a well-suited model organism (chicken), we fill the remaining gap to characterize its seventh member known from rat as galectin-related inter-fiber protein (GRIFIN) in the lens. Its single-copy gene is common to vertebrates, with one or more deviati ...
DOI:: 10.1016/j.biochi.2016.06.001
: https://doi.org/10.1016/j.biochi.2016.06.001

85. Chromatin-Modifying Agents Reactivate Embryonic Renal Stem/Progenitor Genes in Human Adult Kidney Epithelial Cells but Abrogate Dedifferentiation and Stemness

Author:: Omer Dorit; Harari-Steinberg Orit; Buzhor Ella; Metsuyanim Sally; Pleniceanu Oren; Zundelevich Adi; Gal-Yam Einav Nili; Dekel Benjamin
Source:: Cellular reprogramming 2013 v.15 no.4 pp. 281-292
ISSN:: 2152-4998
Subject:: Western blotting; acetylation; adults; antagonists; cadherins; cell senescence; epigenetics; epithelial cells; flow cytometry; gene activation; gene expression; genes; hematopoietic stem cells; histone deacetylase; histones; induced pluripotent stem cells; kidneys; lysine; messenger RNA; methylation; reverse transcriptase polymerase chain reaction; synergism; valproic acid; vimentin
Abstract:: ... Recent studies have suggested that epigenetic modulation with chromatin-modifying agents can induce stemness and dedifferentiation and increase developmental plasticity. For instance, valproic acid (VPA), a histone deacetylase (HDAC) inhibitor, has been shown to promote self-renewal/expansion of hematopoietic stem cells and facilitate the generation of induced pluripotent stem cells (iPSCs). Previ ...
DOI:: 10.1089/cell.2012.0087
PubMed:: 23841748
PubMed Central:: PMC3725946
: https://doi.org/10.1089/cell.2012.0087

86. Chronic administration tetrahydroxystilbene glucoside promotes hippocampal memory and synaptic plasticity and activates ERKs, CaMKII and SIRT1/miR-134 in vivo

Author:: Tao Chen; Yuan-Jian Yang; Yan-Kun Li; Jing Liu; Peng-Fei Wu; Fang Wang; Jian-Guo Chen; Li-Hong Long
Source:: Journal of ethnopharmacology 2016 v.190 pp. 74-82
ISSN:: 0378-8741
Subject:: Oriental traditional medicine; Polygonum; Reynoutria multiflora; Western blotting; adults; anti-aging properties; calcium-calmodulin-dependent protein kinase; electrophysiology; fearfulness; glucosides; hippocampus; memory; mice; mitogen-activated protein kinase; neuroplasticity; phosphorylation; polyphenols; quantitative polymerase chain reaction; staining
Abstract:: ... Polygonum multiflorum Thunb is a traditional Chinese medicine with anti-aging effect. 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (TSG) is generally considered as the main active component in Polygonum multiflorum Thunb. However, the effect of TSG on memory in adult is unclear till now.2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside (TSG) is a polyphenols compound from Polygonum multiﬂorum Thu ...
DOI:: 10.1016/j.jep.2016.06.012
: https://doi.org/10.1016/j.jep.2016.06.012

87. Chronic ethanol exposure induces neuroinflammation in H4 cells through TLR3 / NF-κB pathway and anxiety-like behavior in male C57BL/6 mice

Author:: Xiaolong Wang; Hao Yu; Changliang Wang; Yang Liu; Jiabin You; Pengfei Wang; Guohui Xu; Hui Shen; Hui Yao; Xinze Lan; Rui Zhao; Xu Wu; Guohua Zhang
Source:: Toxicology 2020 v.446 pp. 152625
ISSN:: 0300-483X
Subject:: RNA interference; Toll-like receptor 3; Toll-like receptor 4; Western blotting; adults; alcohol abuse; ammonium; animal experimentation; cell culture; culture media; drinking; elevated plus-maze test; enzyme-linked immunosorbent assay; ethanol; genes; glioma; hippocampus; human diseases; interleukin-1beta; interleukin-6; males; mice; neoplasm cells; public health; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:: ... Chronic alcoholism has become a major public health problem. Long-term and excessive drinking can lead to a variety of diseases. Chronic ethanol exposure can induce neuroinflammation and anxiety-like behavior, and this may be induced through the Toll-like receptor 3/nuclear factor-κB (TLR3/NF-κB) pathway. Animal experiments were performed using healthy adult male C57BL/6 N mice given 10 % (m/V) or ...
DOI:: 10.1016/j.tox.2020.152625
: https://doi.org/10.1016/j.tox.2020.152625

88. Chronic inhibition of nuclear factor kappa B attenuates aldosterone/salt-induced renal injury

Author:: Wei Ding; Lei Yang; Minmin Zhang; Yong Gu
Source:: Life sciences 2012 v.90 no.15-16 pp. 600-606
ISSN:: 0024-3205
Subject:: Western blotting; adults; aldosterone; collagen; ethanol; gene expression regulation; hypertension; immunohistochemistry; messenger RNA; pathogenesis; protein synthesis; quantitative polymerase chain reaction; rats; signal transduction; sodium chloride; transcription factor NF-kappa B; transforming growth factor beta
Abstract:: ... AIMS: Recent studies suggested that nuclear factor kappa B (NF-κB) plays a key role in the pathogenesis of renal injury. This study investigated whether NF-κB inhibition attenuates progressive renal damage in aldosterone/salt-induced renal injury and its mechanisms. MAIN METHODS: Adult male rats were uninephrectomized and treated with one of the following for 4weeks: vehicle (0.5% ethanol, subcuta ...
DOI:: 10.1016/j.lfs.2012.02.022
: https://doi.org/10.1016/j.lfs.2012.02.022

89. Chronic social stress induces cardiomyocyte contractile dysfunction and intracellular Ca²⁺ derangement in rats

Author:: Subat Turdi; Ming Yuan; Gail M. Leedy; Zhenbiao Wu; Jun Ren
Source:: Physiology & behavior 2012 v.105 no.2 pp. 498-509
ISSN:: 0031-9384
Subject:: Ca2-transporting ATPase; Western blotting; phosphorylation; mitogen-activated protein kinase; oxidative stress; adults; fibrosis; fluorescence; psychological stress; phenylephrine; calcium; histology; superoxide dismutase; models; psychosocial factors; rats; antioxidants; cardiovascular diseases; cardiomyocytes; reticulum; hypertrophy
Abstract:: ... Chronic psychosocial stress triggers cardiovascular diseases although underlying mechanisms are still elusive. This study examined the effect of social stress on cardiomyocyte contractile function and pathological changes in myocardium using the visible burrow system (VBS) model. Chronic social stress was induced using a mixed-sex VBS housing in adult Sprague–Dawley (SD) rats. Contractile and intr ...
DOI:: 10.1016/j.physbeh.2011.09.012
PubMed:: 21952229
PubMed Central:: PMC3225694
: https://doi.org/10.1016/j.physbeh.2011.09.012

90. Cloning and Characterization of a Novel, Human Cellular Retinaldehyde-binding Protein CRALBP-like (CRALBPL) Gene

Author:: Kong, Ya-Hui; Ye, Guang-Ming; Qu, Kai; Pan, Wen-Qi; Liu, Xiang-Hua; Wan, Bo; Guo, Jin-Hu; Yu, Long
Source:: Biotechnology letters 2006 v.28 no.17 pp. 1327-1333
ISSN:: 0141-5492
Subject:: Western blotting; adults; amino acids; brain; cDNA libraries; complementary DNA; genes; humans; molecular weight; open reading frames
Abstract:: ... Cellular retinaldehyde-binding protein (CRALBP) plays a role in the vertebrate visual process as a substrate-routing protein. It belongs to a widespread lipid-binding SEC14-like protein family. All the members of the family have the lipid-binding domain called CRAL-TRIO. Here we have isolated a new human CRAL-TRIO domain containing a CRALBP-like (CRALBPL) gene from the cDNA library of human adult ...
DOI:: 10.1007/s10529-006-9096-5
PubMed:: 16802092
: https://doi.org/10.1007/s10529-006-9096-5

91. Cloning and characterization of a cDNA fragment encoding a Schistosoma mansoni actin-binding protein (Smfilamin)

Author:: M. R. Mohamed; K. A. Shalaby; P. T. LoVerde; N. M. Abd Allah; A. M. Karim
Source:: Parasitology research 2008 v.102 no.5 pp. 1035-1042
ISSN:: 0932-0113
Subject:: Northern blotting; Schistosoma mansoni; Western blotting; adults; amino acids; antiserum; cDNA libraries; clones; complementary DNA; filamin; fluorescence; fluorescent antibody technique; genes; humans; immunoglobulin G; rabbits; reverse transcriptase polymerase chain reaction; schistosomula
Abstract:: ... To identify vaccine candidates for Schistosoma mansoni, the IgG fraction of rabbit antiserum raised against immature female worms affinity purified over a NP-40 extract of 3-h schistosomula was used to immunoscreen a cercarial λgt11 cDNA library. One clone with a 1.5-kb cDNA insert revealed an encoded peptide of 479 amino acids, which bears homology to human actin-binding protein (ABP-280 = filami ...
DOI:: 10.1007/s00436-007-0872-5
PubMed:: 18283496
: https://doi.org/10.1007/s00436-007-0872-5

92. Cloning and characterization of thioredoxin peroxidases from Trichinella spiralis

Author:: N.Z. Zhang; J.Y. Liu; W.H. Li; L. Li; Z.G. Qu; T.T. Li; J.M. Cui; Y. Yang; W.Z. Jia; B.Q. Fu
Source:: Veterinary parasitology 2016 v.231 pp. 53-58
ISSN:: 0304-4017
Subject:: Trichinella spiralis; Western blotting; adults; gene expression; gene expression regulation; genes; helminths; hydrogen peroxide; muscle larvae; neonates; open reading frames; oxidative stress; peroxidases; proteins; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; swine
Abstract:: ... The intracellular parasitic nematode, Trichinella spiralis, can initiate a high level of oxidative stress, especially during rapid growth and generative propagation phases. Thioredoxin peroxidases (TPXs) protect helminths against oxidative stress, but none has been identified in T. spiralis. Here, 3 members of the TPX family were cloned from T. spiralis muscle larvae (ML). The lengths of TsTPX ORF ...
DOI:: 10.1016/j.vetpar.2016.05.027
: https://doi.org/10.1016/j.vetpar.2016.05.027

93. Cloning and expression analysis of a G‐protein subunit— in the rice water weevil lissorhoptrus oryzophilus Kuschel

Author:: Kang, Gui‐Juan; Gong, Zhong‐Jun; Cheng, Jia‐An; Mao, Cun‐Gui; Zhu, Zeng‐Rong
Source:: Archives of insect biochemistry and physiology 2011 v.76 no.1 pp. 43-54
ISSN:: 0739-4462
Subject:: G-proteins; Lissorhoptrus oryzophilus; Western blotting; adults; amino acids; antennae; dendrites; messenger RNA; molecular weight; open reading frames; parthenogenesis; quantitative polymerase chain reaction; sensilla; signal transduction
Abstract:: ... The open reading frame (ORF) encoding a novel G protein α subunit, Lo Gαo, was cloned from the parthenogenetic rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae). The Lo Gαo ORF encodes a protein of 354 amino acid residues. The deduced protein sequence shares high homology with Gαo from other species. The expression patterns of Lo Gαo in various adult tissues were ind ...
DOI:: 10.1002/arch.20403
: https://doi.org/10.1002/arch.20403

94. Cloning and expression of 21.1-kDa tegumental protein of Clonorchis sinensis and human antibody response to it as a trematode-nematode pan-specific serodiagnosis antigen

Author:: Chen, Jiajia; Xu, Hongxu; Zhang, Zhaoping; Zeng, Suxiang; Gan, Wenjia; Yu, Xinbing; Hu, Xuchu
Source:: Parasitology research 2011 v.108 no.1 pp. 161-168
ISSN:: 0932-0113
Subject:: Clonorchis sinensis; Escherichia coli; Fasciola hepatica; Nematoda; Schistosoma japonicum; Toxoplasma gondii; Western blotting; adults; affinity chromatography; antigens; bioinformatics; complementary DNA; cross reaction; enzyme-linked immunosorbent assay; humans; immunoglobulin G; immunohistochemistry; nematode infections; patients; plasmids; serodiagnosis
Abstract:: ... A complete cDNA encoding a 21.1-kDa tegumental protein (CsTP21.1) was recognized from Clonorchis sinensis adult full-length cDNA plasmid library by bioinformatics analysis. Recombinant CsTP21.1 was highly expressed in Escherichia coli, purified by affinity chromatography, and identified by Western blotting. Immunohistochemistry demonstrated that CsTP21.1 is localized in the tegument of the adult w ...
DOI:: 10.1007/s00436-010-2050-4
PubMed:: 20865432
: https://doi.org/10.1007/s00436-010-2050-4

95. Cloning and expression of a 16-kDa recombinant protein from Angiostrongylus cantonensis for use in immunoblot diagnosis of human angiostrongyliasis

Author:: Apichat Vitta; Paron Dekumyoy; Chalit Komalamisra; Thareerat Kalambaheti; Timothy P. Yoshino
Source:: Parasitology research 2016 v.115 no.11 pp. 4115-4122
ISSN:: 0932-0113
Subject:: Gnathostoma; Parastrongylus cantonensis; Taenia solium; Western blotting; adults; antibodies; antigens; blood serum; clones; complementary DNA; humans; meningoencephalitis; molecular weight; parasites; patients; peptides; protein synthesis; recombinant proteins; serodiagnosis
Abstract:: ... Angistrongylus cantonensis is a zoonotic nematode parasite and causative agent of human angiostrongyliasis, which clinically presents as eosinophilic meningitis or meningoencephalitis. Diagnosis of the disease is problematic since parasitologic findings are infrequent, and infection determinations must be based on the clinical symptoms and serological tests with limited specificities and sensitivi ...
DOI:: 10.1007/s00436-016-5184-1
: https://doi.org/10.1007/s00436-016-5184-1

96. Cloning and expression of a Trichinella spiralis putative glutathione S-transferase and its elicited protective immunity against challenge infections

Author:: Chun Ying Liu; Yan Yan Song; Hua Na Ren; Ge Ge Sun; Ruo Dan Liu; Peng Jiang; Shao Rong Long; Xi Zhang; Zhong Quan Wang; Jing Cui
Source:: Parasites & vectors 2017 v.10 no.1 pp. 448
ISSN:: 1756-3305
Subject:: Escherichia coli; Trichinella spiralis; Western blotting; adults; amino acid sequences; amino acids; antibodies; antigens; blood serum; complementary DNA; enzyme activity; enzyme-linked immunosorbent assay; fluorescent antibody technique; glutathione transferase; immune response; immunoglobulin G; mice; molecular weight; multigene family; muscle larvae; neonates; nucleotide sequences; reverse transcriptase polymerase chain reaction; spectroscopy; vaccination; vaccines
Abstract:: ... BACKGROUND: Glutathione-S-transferase (GST) is a widespread multigene family of detoxification enzymes. The vaccination of mice with recombinant GST of 24 kDa from Trichinella spiralis elicited a low immune protection against challenge infection. The objective of this study was to characterize the T. spiralis putative GST gene (TspGST) encoding a 30.8 kDa protein and to evaluate its potential as a ...
DOI:: 10.1186/s13071-017-2384-1
PubMed:: 28962639
PubMed Central:: PMC5622431
: https://doi.org/10.1186/s13071-017-2384-1

97. Cloning of cDNA encoding a Bombyx mori homolog of human oxidation resistance 1 (OXR1) protein from diapause eggs, and analyses of its expression and function

Author:: Noriko Kobayashi; Masaki Takahashi; Shouhei Kihara; Teruyuki Niimi; Okitsugu Yamashita; Toshinobu Yaginuma
Source:: Journal of insect physiology 2014 v.68 pp. 58-68
ISSN:: 0022-1910
Subject:: silkworms; Western blotting; blastoderm; insect physiology; adults; hydrogen peroxide; males; gene expression; diapause; mitochondria; oxidation; gene expression regulation; Drosophila melanogaster; messenger RNA; complementary DNA; humans; fruit flies; double-stranded RNA; antioxidant activity; eggs; quantitative polymerase chain reaction; diet; rearing; reverse transcriptase polymerase chain reaction; Bombyx mori
Abstract:: ... To better understand the molecular mechanisms of diapause initiation, we used the sensitive cDNA subtraction (selective amplification via biotin- and restriction-mediated enrichment) method and isolated a novel gene expressed abundantly in diapause eggs of the silkworm, Bombyx mori, which encodes a homolog of the human oxidation resistance 1 (OXR1) protein. Quantitative real-time polymerase chain ...
DOI:: 10.1016/j.jinsphys.2014.06.020
: https://doi.org/10.1016/j.jinsphys.2014.06.020

98. Cloning, characterisation and expression profiling of the cDNA encoding the ryanodine receptor in diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

Author:: Lei Guo; Baozhen Tang; Wei Dong; Pei Liang; Xiwu Gao
Source:: Pest management science 2012 v.68 no.12 pp. 1605-1614
ISSN:: 1526-498X
Subject:: Plutella xylostella; Western blotting; adults; alternative splicing; amino acids; antibodies; complementary DNA; digestive system; genes; head; insecticide resistance; insecticides; integument; larvae; open reading frames; prepupae; pupae; rapid amplification of cDNA ends; receptors; resistance mechanisms; reverse transcriptase polymerase chain reaction
Abstract:: ... BACKGROUND: The rynodine receptors (RyRs) are the main targets of diamide insecticides such as chlorantraniliprole. To provide the basis for a good understanding of the molecular mechanisms of diamide insecticide resistance, an RyR gene from Plutella xylostella was cloned and characterised in the present paper. RESULTS: A full‐length cDNA sequence of RyR was cloned from P. xylostella through RT‐PC ...
DOI:: 10.1002/ps.3357
PubMed:: 22761165
: https://doi.org/10.1002/ps.3357

99. Cloning, expression and characterization of a Trichinella spiralis serine protease gene encoding a 35.5kDa protein

Author:: Bin Wang; Zhong Quan Wang; Jing Jin; Hui Jun Ren; Li Na Liu; Jing Cui
Source:: Experimental parasitology 2013 v.134 no.2 pp. 148-154
ISSN:: 0014-4894
Subject:: Escherichia coli; Trichinella spiralis; Western blotting; adults; antigens; blood serum; excretory-secretory products; genes; immunity; intestinal mucosa; mice; muscle larvae; muscles; neonates; parasites; parasitology; serine proteinases; vaccines
Abstract:: ... Serine proteases are found in the excretory-secretory (ES) products from Trichinella spiralis muscle larvae, have collagenolytic and elastolytic activities, and may be related to the larval invasion of intestinal epithelial cells. In this study, the serine protease gene (TspSP-1.2, GenBank accession No. EU302800) encoding a 35.5kDa protein from T. spiralis was cloned, and recombinant TspSP-1.2 pro ...
DOI:: 10.1016/j.exppara.2013.03.004
: https://doi.org/10.1016/j.exppara.2013.03.004

100. Cloning, expression and functional analysis of a general odorant-binding protein 2 gene of the rice striped stem borer, Chilo suppressalis (Walker) (Lepidoptera: Pyralidae)

Author:: Gong, Z.-J.; Zhou, W.-W.; Yu, H.-Z.; Mao, C.-G.; Zhang, C.-X.; Cheng, J.A.; Zhu, Z.-R.
Source:: Insect molecular biology 2009 v.18 no.3 pp. 405-417
ISSN:: 0962-1075
Subject:: Chilo suppressalis; Escherichia coli; Pyralidae; Western blotting; adults; affinity chromatography; amino acids; antennae; benzaldehyde; binding proteins; complementary DNA; females; fluorescence; gel chromatography; genes; males; messenger RNA; odor compounds; open reading frames; pheromones; rapid amplification of cDNA ends; reverse transcriptase polymerase chain reaction; rice
Abstract:: ... A full-length cDNA encoding a general odorant binding protein 2 (GOBP2) was cloned from the antennae of the rice striped stem borer, Chilo suppressalis (Lepidoptera: Pyralidae), by the combination of reverse transcription PCR (RT-PCR) and rapid amplification of cDNA ends PCR (RACE-PCR). The cDNA contains a 489 bp open reading frame, which encodes a 162 amino acid protein, termed as Ch. suppressali ...
DOI:: 10.1111/j.1365-2583.2009.00886.x
PubMed:: 19523072
: https://doi.org/10.1111/j.1365-2583.2009.00886.x

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1. 2,5-Hexanedione altered the degradation of low-molecular-weight neurofilament in rat nerve tissues

2. 20(S)-Ginsenoside Rh2 Induce the Apoptosis and Autophagy in U937 and K562 Cells

3. 41.5-kDa Cathepsin L protease from Clonorchis sinensis: expression, characterization, and serological reactivity of one excretory–secretory antigen

4. A Serological Survey About Zoonoses in the Verkhoyansk Area, Northeastern Siberia (Sakha Republic, Russian Federation)

5. A Sertoli cell-specific connexin43 knockout leads to altered interstitial connexin expression and increased Leydig cell numbers

6. A glutathione S-transferase gene associated with antioxidant properties isolated from Apis cerana cerana

7. A kinetic characterization of the gill V(H+)-ATPase in juvenile and adult Macrobrachium amazonicum, a diadromous palaemonid shrimp

8. A methyl-deficient diet fed to rats during the pre- and peri-conception periods of development modifies the hepatic proteome in the adult offspring

9. A proteomic analysis of Nipponia nippon (ID#162)

10. A rapid, targeted, neuron-selective, in vivo knockdown following a single intracerebroventricular injection of a novel chemically modified siRNA in the adult rat brain

11. A salivary sheath protein essential for the interaction of the brown planthopper with rice plants

12. A splicing variant leads to complete loss of function of betaine–homocysteine methyltransferase (BHMT) gene in hepatocellular carcinoma

13. ATF3 Expression in the Corpus Luteum: Possible Role in Luteal Regression†

14. Aberrant fatty acid metabolism in skeletal muscle contributes to insulin resistance in zinc transporter 7 (znt7)-knockout mice

15. Acid-sensing ion channels (ASICs) 2 and 4.2 are expressed in the retina of the adult zebrafish

16. Activation of calcium-sensing receptor increases TRPC3 expression in rat cardiomyocytes

17. Activation of the Wnt/β-catenin signaling pathway is associated with glial proliferation in the adult spinal cord of ALS transgenic mice

18. Acute effects of ethanol on feeding behavior and leptin-induced STAT3 phosphorylation in rat hypothalamus

19. Adverse Effects of Rice gall dwarf virus upon its Insect Vector Recilia dorsalis (Hemiptera: Cicadellidae)

20. Age-related changes in the morphology and protein expression of the thymus of healthy yaks (Bos grunniens)

21. Aging and retinoid X receptor agonists on masculinization of female Pomacea canaliculata, with a critical appraisal of imposex evaluation in the Ampullariidae

22. Alteration in iron efflux affects male sex hormone testosterone biosynthesis in a diet-induced obese rat model

23. Alterations of protein expression in serum of infants with intrauterine growth restriction and different gestational ages

24. Ameliorative effect of Gastrodia elata Blume extracts on depression in zebrafish and cellular models through modulating reticulon 4 receptors and apoptosis

25. Amphibian larvae and zinc sulphate: a suitable model to study the role of brain-derived neurotrophic factor (BDNF) in the neuronal turnover of the olfactory epithelium

26. An antigenic recombinant serine protease from Trichinella spiralis induces protective immunity in BALB/c mice

27. Analysis of OCT4 expression in an extended panel of human tumor cell lines from multiple entities and in human mesenchymal stem cells

28. Analysis of an expressed sequence tag library from Dicrocoelium dentriticum

29. Analysis of the developmental expression of small VCP-interacting protein and its interaction with steroidogenic acute regulatory protein in Leydig cells

30. Analysis of the stem cell characteristics of adult stem cells from Arbas white Cashmere goat

31. Analysis of the structural proteins from the Musca domestica hytrosavirus with an emphasis on the major envelope protein

32. Androgen receptor, aromatase, oestrogen receptor α/β and G protein‐coupled receptor 30 expression in the testes and epididymides of adult sheep

33. Angiogenic properties of adult human thymus fat

34. Anoctamin 1 expression in the mouse auditory brainstem

35. Anti-Müllerian hormone receptor type 2 (AMHR2) expression in bovine oviducts and endometria: comparison of AMHR2 mRNA and protein abundance between old Holstein and young and old Wagyu females

36. Aquaporin AQP11 in the testis: molecular identity and association with the processing of residual cytoplasm of elongated spermatids

37. ArHsp22, a developmentally regulated small heat shock protein produced in diapause-destined Artemia embryos, is stress inducible in adults

38. Arginine kinase from Haemonchus contortus decreased the proliferation and increased the apoptosis of goat PBMCs in vitro

39. Aristoyunnolin H attenuates extracellular matrix secretion in cardiac fibroblasts by inhibiting calcium influx

40. Astragaloside IV inhibits Angiotensin II-stimulated proliferation of rat vascular smooth muscle cells via the regulation of CDK2 activity

41. Autophagic activity in the mouse urinary bladder urothelium as a response to starvation

42. Autophagy can repair endoplasmic reticulum stress damage of the passive Heymann nephritis model as revealed by proteomics analysis

43. Autophagy of hepatic stellate cell induced by Clonorchis sinensis

44. BRCA-1 Gene Expression and Comparative Proteomic Profile of Primordial Follicles from Young and Adult Buffalo (Bubalus bubalis) Ovaries

45. Berberine protects against ischemia/reperfusion injury after orthotopic liver transplantation via activating Sirt1/FoxO3α induced autophagy

46. Bio-guided identification of proteins for the diagnosis of cysticercosis in swine

47. Bioinformatics, tissue distribution, and subcellular localization analyses of fk506 binding protein 12b from silkworms

48. Bisphenol A and dibutyl phthalate affect the expression of juxtacrine signaling factors in rat testis

49. Bisphenol A induces spermatocyte apoptosis in rare minnow Gobiocypris rarus

50. CCDC134, a novel secretory protein, inhibits activation of ERK and JNK, but not p38 MAPK

51. Can the activation of plasminogen/plasmin system of the host by metabolic products of Dirofilaria immitis participate in heartworm disease endarteritis?

52. Capsaicin protects against testicular torsion injury through mTOR-dependent mechanism

53. Caracterization of glutathione S-transferase of Dermacantor marginatus and effect of the recombinant antigen as a potential anti-tick vaccine

54. Cellular Distribution of Anionic Antimicrobial Peptide in Normal Lung and during Acute Pulmonary Inflammation

55. Characterisation of major vault protein during the life cycle of the human parasite Schistosoma mansoni

56. Characteristics and function of cathepsin L3 from Schistosoma japonicum

57. Characterization and dietary regulation of oligopeptide transporter (PepT1) in different ploidy fishes

58. Characterization and expression analysis of an intelectin gene from Megalobrama amblycephala with excellent bacterial binding and agglutination activity

59. Characterization and expression of cathepsin B2 in Fasciola gigantica

60. Characterization and localization of antigens for serodiagnosis of human paragonimiasis

61. Characterization of Melanoplus sanguinipes oviposition stimulating protein expression and re-examination of its potential role in stimulating oviposition

62. Characterization of Ostertagia ostertagi annexin-like proteins at different developmental stages

63. Characterization of TsDAF-21/HSP90 protein from the parasitic nematode Trichinella spiralis

64. Characterization of TsMRP-L28, a mitochondrial ribosomal protein L28 from the parasitic nematode Trichinella spiralis

65. Characterization of a Trichinella spiralis 31kDa protein and its potential application for the serodiagnosis of trichinellosis

66. Characterization of a Trichinella spiralis cathepsin X and its promotion for the larval invasion of mouse intestinal epithelial cells

67. Characterization of a chymotrypsin-like enzyme from Trichinella spiralis and its facilitation of larva penetration into the host's enteral epithelial cells

68. Characterization of a novel aspartyl protease inhibitor from Haemonchus contortus

69. Characterization of a piRNA binding protein Miwi in mouse oocytes

70. Characterization of a secreted cystatin from the tick Rhipicephalus haemaphysaloides