Subject: Western blotting - PubAg Search Results

1. Development and evaluation of recombinant GRA8 protein for the serodiagnosis of Toxoplasma gondii infection in goats

Author:: Jirapattharasate, Charoonluk; Udonsom, Ruenruetai; Prachasuphap, Apichai; Jongpitisub, Kodcharad; Dhepakson, Panadda
Source:: BMC veterinary research 2021 v.17 no.1 pp. 27
ISSN:: 1746-6148
Subject:: Escherichia coli; Toxoplasma gondii; Western blotting; antibodies; antigens; blood serum; cell culture; enzyme-linked immunosorbent assay; extraction; genomics; goats; humans; infection; laboratory animals; latex agglutination test; molecular weight; plasmids; polyacrylamide gel electrophoresis; protein synthesis; recombinant proteins; sampling; serodiagnosis; synthesis; synthetic genes; tachyzoites; toxoplasmosis; veterinary medicine
Abstract:: ... BACKGROUND: The development of sensitive and specific methods for detecting Toxoplasma gondii infection is critical for preventing and controlling toxoplasmosis in humans and other animals. Recently, various recombinant proteins have been used in serological tests for diagnosing toxoplasmosis. The production of these antigens is associated with live tachyzoites obtained from cell cultures or labor ...
DOI:: 10.1186/s12917-020-02719-3
: https://dx.doi.org/10.1186/s12917-020-02719-3

2. Identification of a universal antigen epitope of influenza A virus using peptide microarray

Author:: Wang, Qiuxia; Sun, Zhihao; Li, Jingzhi; Qin, Tao; Ma, Hongwei; Chen, Sujuan; Peng, Daxin; Liu, Xiufan
Source:: BMC veterinary research 2021 v.17 no.1 pp. 22
ISSN:: 1746-6148
Subject:: Anas platyrhynchos; CD4-positive T-lymphocytes; Influenza A virus; Western blotting; amino acid substitution; amino acids; antiserum; blood serum; databases; detection; epitopes; hemagglutinins; interleukin-2; peptide microarrays; silica gel; surface proteins; vaccine development; veterinary medicine; viruses
Abstract:: ... BACKGROUND: Hemagglutinin is a major surface protein in influenza A virus (IAV), and HA2 is relative conserved among different IAVs. It will be meaningful to identify broad-spectrum epitopes based on the HA2 protein. RESULTS: Overlapping peptides of the HA2 protein of the H5N1 IAV A/Mallard/Huadong/S/2005 were synthesized and loaded on modified silica gel film to form a microarray, and antisera ag ...
DOI:: 10.1186/s12917-020-02725-5
: https://dx.doi.org/10.1186/s12917-020-02725-5

3. Filamin A is required for somatostatin receptor type 5 expression and pasireotide-mediated signaling in pituitary corticotroph tumor cells

Author:: Treppiedi, Donatella; Di Muro, Genesio; Mangili, Federica; Catalano, Rosa; Giardino, Elena; Barbieri, Anna Maria; Locatelli, Marco; Arosio, Maura; Spada, Anna; Peverelli, Erika; Mantovani, Giovanna
Source:: Molecular and cellular endocrinology 2021 v.524 pp. 111159
ISSN:: 0303-7207
Subject:: Western blotting; apoptosis; cell proliferation; corticotropin; cytoskeleton; filamin; hormone secretion; humans; mice; neoplasm cells; neoplasms; somatostatin; somatostatin receptors
Abstract:: ... Somatostatin receptor type 5 (SST5) represents the main pharmacological target in the treatment of adrenocorticotroph hormone (ACTH)-secreting tumors. However, molecular predictors of responsiveness to pasireotide require further investigation. The cytoskeleton protein filamin A (FLNA) modulates the responsiveness to somatostatin analogs (SSA) treatment in other types of pituitary tumors by regula ...
DOI:: 10.1016/j.mce.2021.111159
: https://dx.doi.org/10.1016/j.mce.2021.111159

4. A lipid-soluble extract of Pinellia pedatisecta Schott orchestrates intratumoral dendritic cell-driven immune activation through SOCS1 signaling in cervical cancer

Author:: Wang, Yumeng; Lu, Chong; Huang, Haixia; Yao, Sheng; Xu, Congjian; Ye, Yang; Gui, Suiqi; Li, Guiling
Source:: Journal of ethnopharmacology 2021 v.267 pp. 112837
ISSN:: 0378-8741
Subject:: Pinellia; Western blotting; apoptosis; biomarkers; bone marrow; dendritic cells; drugs; flow cytometry; gene silencing; genetic markers; humans; interleukin-12; medicinal plants; mice; models; neoplasm cells; non-specific protein-tyrosine kinase; phenotype; phosphorylation; quantitative polymerase chain reaction; traditional medicine; uterine cervical neoplasms
Abstract:: ... Pinellia pedatisecta Schott extract (PE) is generated from Pinellia pedatisecta Schott, a traditional Chinese medicinal plant. PE suppresses cervical tumor growth and exhibits effects on dendritic cells (DCs) that lead to modulation of antitumor CD4⁺ and CD8⁺ responses.To explore the underlying mechanisms by which PE modulates tumor-associated dendritic cell (TADC) activation and function.DCs and ...
DOI:: 10.1016/j.jep.2020.112837
: https://dx.doi.org/10.1016/j.jep.2020.112837

5. Activation of MAPK signaling in response to nitrogen deficiency in Ulva prolifera (Chlorophyta)

Author:: Yang, Juanjuan; Yin, Yi; Yu, Dachun; He, Lihong; Shen, Songdong
Source:: Algal research 2021 v.53 pp. 102153
ISSN:: 2211-9264
Subject:: Ulva prolifera; Western blotting; algal blooms; apoptosis; assays; fluorescence; glutamine; macroalgae; mortality; nitrate reductase; nitrite reductase; nitrogen; protoplasts; research; staining
Abstract:: ... Ulva prolifera (Ulvophyceae, Chlorophyta), one of the main seaweeds species that cause “green tides”, suffers changes in nitrogen levels during drifting. However, the mechanisms of how this alga resist to nitrogen deficiency remains unclear. Transcripts encoding nitrate reductase, nitrite reductase, and glutamine synthase involved in nitrogen assimilation were amplified by RT-PCR, suggesting that ...
DOI:: 10.1016/j.algal.2020.102153
: https://dx.doi.org/10.1016/j.algal.2020.102153

6. Characterisation of novel regulatory sequences compatible with modular assembly in the diatom Phaeodactylum tricornutum

Author:: Windhagauer, Matthias; Abbriano, Raffaela M.; Ashworth, Justin; Barolo, Lorenzo; Jaramillo-Madrid, Ana Cristina; Pernice, Mathieu; Doblin, Martina A.
Source:: Algal research 2021 v.53 pp. 102159
ISSN:: 2211-9264
Subject:: Phaeodactylum tricornutum; Western blotting; algae; databases; flow cytometry; fluorescence; gene expression; genetic engineering; genome; heterologous gene expression; metabolic engineering; models; nitrogen; photoperiod; plasmids; promoter regions; protein content; research; synthetic biology; transcriptomics
Abstract:: ... Phaeodactylum tricornutum is a model pennate diatom for molecular studies due to its sequenced genome, genetic tractability, and capacity to produce a myriad of compounds of biotechnological interest. While tools for genetic engineering and heterologous gene expression have been developed for this species, the available set of characterised promoters remains limited. A diverse and well-understood ...
DOI:: 10.1016/j.algal.2020.102159
: https://dx.doi.org/10.1016/j.algal.2020.102159

7. Validation of a monoclonal antibody directed against the human sphingosine 1-phosphate receptor type 1

Author:: Thuy, Andreas V.; Jeya Paul, Jefri; Weigel, Cynthia; Ziegler, Anke C.; Guntinas-Lichius, Orlando; Gräler, Markus H.
Source:: Journal of immunological methods 2021 v.490 pp. 112953
ISSN:: 0022-1759
Subject:: B-lymphocytes; Western blotting; agonists; antagonists; antibody specificity; cell lines; epitopes; flow cytometry; human umbilical vein endothelial cells; humans; immunoglobulin M; mice; monoclonal antibodies; precipitin tests; sphingosine; staining; tonsils
Abstract:: ... The sphingosine 1-phosphate receptor type 1 (S1PR1) has several important functions, including stabilizing endothelial barrier and maintaining lymphocyte circulation. These functions are critically dependent on the regulation of S1PR1 cell surface expression. Currently available antibodies against human S1PR1 are not able to pick up cell surface expression on living cells by flow cytometry due to ...
DOI:: 10.1016/j.jim.2020.112953
: https://dx.doi.org/10.1016/j.jim.2020.112953

8. Ozone exposure promotes pyroptosis in rat lungs via the TLR2/4-NF-κB-NLRP3 signaling pathway

Author:: Tian, Lei; Yan, Jun; Li, Kang; Zhang, Wei; Lin, Bencheng; Lai, Wenqing; Bian, Liping; Liu, Huanliang; Xi, Zhuge; Liu, Xiaohua
Source:: Toxicology 2021 v.450 pp. 152668
ISSN:: 0300-483X
Subject:: Toll-like receptors; Western blotting; air pollutants; animal experimentation; assays; blood; caspase-1; edema; epithelial cells; females; histopathology; hyaluronic acid; inflammasomes; inflammation; lungs; males; nucleoproteins; ozone; pyroptosis; rats; respiratory tract diseases; signal transduction; toxicology; transcription factor NF-kappa B
Abstract:: ... Ozone has become a major air pollutant in recent years, which leading to a variety of lung diseases. This study aimed to explore the mechanisms of pyroptosis and related signaling pathways in ozone-induced lung injury.We exposed 120 Wistar rats to ozone, 20 in each group (half male and half female). Ozone exposure concentrations were 0, 0.12, 0.5, 1.0, 2.0 and 4.0 ppm for 6 h. At the same time, we ...
DOI:: 10.1016/j.tox.2020.152668
: https://dx.doi.org/10.1016/j.tox.2020.152668

9. Identification of lipidomic responses to GCRV infection in Ctenopharyngodon idellus kidney (CIK) cells

Author:: Zhang, Xing; Huang, Jingcai; Shen, Zeen; Wang, Yan; Wang, Yujun; Gong, Chengliang; Hu, Xiaolong; Wang, Chonglong
Source:: Aquaculture 2021 v.533 pp. 736106
ISSN:: 0044-8486
Subject:: Ctenopharyngodon idella; Reoviridae; Western blotting; aquaculture; autophagy; biochemical pathways; droplets; financial economics; fish diseases; immune response; kidney cells; kidneys; lipid composition; lipid metabolism; lipidomics; pathogens; staining; triacylglycerols; virus replication; viruses
Abstract:: ... Grass carp reovirus (GCRV) is a causing pathogen of grass carp hemorrhagic disease, whose outbreak often brings out a huge economic loss in aquaculture filed. Cellular lipids play crucial roles in many biological processes, such as virus replication, maturation and transmission. Presently, it remains to elucidate the roles of these lipids in the GCRV infection. In this study, Here, a lipidomic app ...
DOI:: 10.1016/j.aquaculture.2020.736106
: https://dx.doi.org/10.1016/j.aquaculture.2020.736106

10. Development and characterization of novel cell lines from kidney and eye of rainbow trout, Oncorhynchus mykiss for virological studies

Author:: Suryakodi, S.; Majeed, S. Abdul; Taju, G.; Vimal, S.; Sivakumar, S.; Ahmed, A. Nafeez; Shah, Feroz Ahmad; Bhat, Saqib Ahmad; Sarma, Dandadhar; Begum, Ajima; Hameed, A.S. Sahul
Source:: Aquaculture 2021 v.532 pp. 736027
ISSN:: 0044-8486
Subject:: Cyprinid herpesvirus 2; Nodaviridae; Oncorhynchus mykiss; Tilapia lake virus; Western blotting; cell growth; cell lines; cryopreservation; enzyme-linked immunosorbent assay; epithelium; eyes; fibronectins; fish; fluorescent antibody technique; kidneys; reverse transcriptase polymerase chain reaction; sequence analysis; spleen; viruses; India
Abstract:: ... Two novel cell lines were developed from eye and kidney of rainbow trout, Oncorhynchus mykiss in addition to another cell line from spleen. All these cell lines were characterized and cryopreserved successfully at different passage levels. The cells of eye, kidney and spleen were sub-cultured continuously at an interval of 2–3 days and attained the passage level of 112, 106 and 112, respectively o ...
DOI:: 10.1016/j.aquaculture.2020.736027
: https://dx.doi.org/10.1016/j.aquaculture.2020.736027

11. Development and characterization of polyclonal antibodies against subtype specific vitellogenin of the dojo loach, Misgurnus anguillicaudatus

Author:: Wang, Weilong; Lian, Qingping; Chen, Yuange; Hiramatsu, Naoshi; Wu, Meiqin
Source:: Aquaculture 2021 v.532 pp. 736089
ISSN:: 0044-8486
Subject:: Misgurnus anguillicaudatus; Western blotting; antiserum; aquatic environment; biomarkers; chemiluminescence; estradiol; estrogenic properties; fish; genes; lowest observed effect level; males; polyclonal antibodies; vitellogenin
Abstract:: ... Fish vitellogenin (Vtg) has become an important biomarker for assessing the estrogenic potency of chemicals and the exposure of animals to estrogenic contaminants present in aquatic environments. In the current study, the polyclonal antibodies against subtype-specific Vtgs of the dojo loach (Misgurnus anguillicaudatus) were developed and identified by gene recombination and expression. Immuno-bioc ...
DOI:: 10.1016/j.aquaculture.2020.736089
: https://dx.doi.org/10.1016/j.aquaculture.2020.736089

12. Gli activation by the estrogen receptor in breast cancer cells: Regulation of cancer cell growth by Gli3

Author:: Massah, Shabnam; Foo, Jane; Li, Na; Truong, Sarah; Nouri, Mannan; Xie, Lishi; Prins, Gail S.; Buttyan, Ralph
Source:: Molecular and cellular endocrinology 2021 v.522 pp. 111136
ISSN:: 0303-7207
Subject:: Western blotting; androgen receptors; breast neoplasms; cell growth; coprecipitation; estradiol; estrogen receptors; extracts; genes; ligands; neoplasm cells; precipitin tests; prostatic neoplasms; proteolysis
Abstract:: ... Gli is an oncogenic transcription factor family thought to be involved in breast cancer (BrCa) cell growth. Gli activity is regulated by a post-translational proteolytic process that is suppressed by Hedgehog signaling. In prostate cancer cells, however, Gli activation is mediated by an interaction of active androgen receptor proteins with Gli3 that stabilizes Gli3 in its un-proteolyzed form. Here ...
DOI:: 10.1016/j.mce.2020.111136
: https://dx.doi.org/10.1016/j.mce.2020.111136

13. Polystyrene microplastics cause granulosa cells apoptosis and fibrosis in ovary through oxidative stress in rats

Author:: An, Ru; Wang, Xifeng; Yang, Long; Zhang, Jinjin; Wang, Nana; Xu, Feibo; Hou, Yun; Zhang, Hongqin; Zhang, Lianshuang
Source:: Toxicology 2021 v.449 pp. 152665
ISSN:: 0300-483X
Subject:: Western blotting; acetylcysteine; actin; anti-Mullerian hormone; apoptosis; blood; enzyme-linked immunosorbent assay; females; fibronectins; fibrosis; flow cytometry; granulosa cells; immunohistochemistry; microplastics; muscles; oxidative stress; pollutants; polystyrenes; rats; reactive oxygen species; reproductive toxicology; signal transduction; staining; testing
Abstract:: ... Microplastics (MPs) are receiving increased attention as a harmful environmental pollutant. Studies have investigated that MPs have reproductive toxicity, but the mechanism is little known. Here, we aimed to investigate the effects of polystyrene microplastics (PS-MPs) on ovary in rats and the underlying molecular mechanisms. in vivo, thirty-two female Wistar rats were exposed to 0.5 μm PS-MPs at ...
DOI:: 10.1016/j.tox.2020.152665
: https://dx.doi.org/10.1016/j.tox.2020.152665

14. Anti-depressant effect of Zhi-zi-chi decoction on CUMS mice and elucidation of its signaling pathway

Author:: Chai, Chuan; Jin, Bo; Yan, Yinyu; Yuan, Qi; Wen, Hongmei; Tao, Weiwei; Cui, Xiaobing; Shan, Chenxiao; Yu, Sheng
Source:: Journal of ethnopharmacology 2021 v.266 pp. 113283
ISSN:: 0378-8741
Subject:: Oriental traditional medicine; Western blotting; amino acid metabolism; antidepressants; arginine; aspartic acid; biochemical pathways; biomarkers; blood serum; body weight; cAMP-dependent protein kinase; carbohydrate metabolism; fluoxetine; forced swimming test; gamma-aminobutyric acid; glutamic acid; glutamine; hippocampus; lipid metabolism; males; metabolites; metabolomics; mice; models; pharmacokinetics; phosphatidylcholines; proline; protein synthesis; signal transduction; sucrose; swimming; tail suspension test
Abstract:: ... Zhi-zi-chi decoction (ZZCD) is used for treating depression as an effectively traditional Chinese medicine. Until now, studies on pharmacological research of ZZCD have mostly been centered in pharmacokinetic level. Little was known about its pharmacological mechanism of relieving depression.This study was to evaluate the effect of ZZCD on relieving depression via behavioral tests, serum metabolomi ...
DOI:: 10.1016/j.jep.2020.113283
: https://dx.doi.org/10.1016/j.jep.2020.113283

15. Nanoparticle delivery improves the pharmacokinetic properties of cyclic dinucleotide STING agonists to open a therapeutic window for intravenous administration

Author:: Wehbe, Mohamed; Wang-Bishop, Lihong; Becker, Kyle W.; Shae, Daniel; Baljon, Jessalyn J.; He, Xinyi; Christov, Plamen; Boyd, Kelli L.; Balko, Justin M.; Wilson, John T.
Source:: Journal of controlled release 2021 v.330 pp. 1118-1129
ISSN:: 0168-3659
Subject:: CD8-positive T-lymphocytes; Western blotting; adenocarcinoma; agonists; antibodies; breast neoplasms; encapsulation; gene expression regulation; genes; guanosine; half life; immunosuppression; interferons; intravenous injection; ligands; liver; medicinal properties; melanoma; models; monitoring; nanocapsules; nanocarriers; nanomedicine; nanoparticles; pharmacokinetics; spleen
Abstract:: ... The stimulator of interferon genes (STING) pathway plays an important role in the immune surveillance of cancer and, accordingly, agonists of STING signaling have recently emerged as promising therapeutics for remodeling of the immunosuppressive tumor microenvironment (TME) and enhancing response rates to immune checkpoint inhibitors. 2′3′-cyclic guanosine monophosphate–adenosine monophosphate (2′ ...
DOI:: 10.1016/j.jconrel.2020.11.017
: https://dx.doi.org/10.1016/j.jconrel.2020.11.017

16. Antidiabetic activity of a Flavonoid-Rich extract from flowers of Wisteria sinensis in type 2 diabetic mice via activation of the IRS-1/PI3K/Akt/GLUT4 pathway

Author:: Huang, Yun; Zhou, Tongxi; Zhang, Yirui; Huang, Huiqi; Ma, Yuanren; Wu, Chaoqun; Wang, Qiang; Lin, Qinxiong; Yang, Xinzhou; Pang, Kejian
Source:: Journal of functional foods 2021 v.77 pp. 104338
ISSN:: 1756-4646
Subject:: Western blotting; Wisteria sinensis; adipocytes; fatty liver; flowers; functional foods; glucose; glucose transporters; glycemic effect; histopathology; hyperglycemia; hyperinsulinemia; hyperlipidemia; hypertrophy; insulin; insulin resistance; islets of Langerhans; metabolism; mice; noninsulin-dependent diabetes mellitus; phosphatidylinositol 3-kinase; phosphorylation; plasma membrane; serine; tissues
Abstract:: ... The present study aimed to investigate anti-diabetic activities of a flavonoid-rich extract from Wisteria sinensis (WS-FRE). In vitro, WS-FRE significantly promote the translocation of GLUT4 (Glucose transporter 4) to the plasma membrane. Mechanisms analysis revealed that WS-FRE enhanced glucose uptake via activation of Akt and GLUT4. In vivo, 4-week treatment of WS-FRE alleviated the typical symp ...
DOI:: 10.1016/j.jff.2020.104338
: https://dx.doi.org/10.1016/j.jff.2020.104338

17. Grifola frondosa GF5000 improves insulin resistance by modulation the composition of gut microbiota in diabetic rats

Author:: Xiao, Chun; Jiao, Chunwei; Xie, Yizhen; Ye, Linhui; Li, Qianqing; Wu, Qingping
Source:: Journal of functional foods 2021 v.77 pp. 104313
ISSN:: 1756-4646
Subject:: Grifola frondosa; Western blotting; area; blood glucose; blood lipids; dysbiosis; epididymis; functional foods; genes; glucose tolerance tests; glycemic effect; inflammation; insulin resistance; intestinal microorganisms; intestines; protein content; rats; white adipose tissue
Abstract:: ... The aim was to investigate whether Grifola frondosa fraction (Mw > 5000D) (GF5000) could restore the gut microbiota dysbiosis to improves insulin resistance in diabetic rats. Oral glucose tolerance test and serum biochemical parameter were assessed of the hypoglycemic activity of GF5000 (112.5 and 675 mg/kg/d, i.g.) in diabetic rats for 48 d. The mRNA and protein levels of inflammatory markers in ...
DOI:: 10.1016/j.jff.2020.104313
: https://dx.doi.org/10.1016/j.jff.2020.104313

18. Mitochondrial dynamics in Angiostrongylus cantonensis-infected mouse brain

Author:: Lam, Ho Yin Pekkle; Chen, Cheng-Chi; Chen, Tina Tu-Wen; Chang, Kai-Chih; Wu, Wen-Jui; Yang, Ting-Hua; Liang, Ting-Ruei; Cheng, Po-Ching; Peng, Shih-Yi
Source:: Parasitology international 2021 v.80 pp. 102231
ISSN:: 1383-5769
Subject:: Angiostrongylus; B-cell lymphoma; Parastrongylus cantonensis; Western blotting; apoptosis; biogenesis; brain; caspases; dynamics; exhibitions; infection; inflammation; lead; meningoencephalitis; mice; mitochondria; molecular biology; nucleotidyltransferases; occurrence; parasites; parasitology; pathogenesis; polymerase chain reaction; staining; transmission electron microscopy
Abstract:: ... Angiostrongylus cantonensis is one of the most widespread parasites causing central nervous system (CNS) diseases in mammals. Since the mitochondrion is an essential cell organelle responsible for both physiological and pathological processes, its dysfunction might lead to inflammation and multiple disorders. In this study we aimed to investigate the changes in mitochondrial dynamics that occur in ...
DOI:: 10.1016/j.parint.2020.102231
: https://dx.doi.org/10.1016/j.parint.2020.102231

19. Apigenin attenuates TGF-β1-stimulated cardiac fibroblast differentiation and extracellular matrix production by targeting miR-155-5p/c-Ski/Smad pathway

Author:: Wang, Feng; Fan, Ke; Zhao, Ying; Xie, Mei-Lin
Source:: Journal of ethnopharmacology 2021 v.265 pp. 113195
ISSN:: 0378-8741
Subject:: 3' untranslated regions; Matricaria; Western blotting; actin; apigenin; cardiovascular diseases; chamomile; collagen; enzyme activity; extracellular matrix; fibroblasts; luciferase; muscles; mutants; quantitative polymerase chain reaction; reporter genes; synergism; traditional medicine; transfection; transforming growth factor beta 1
Abstract:: ... Apigenin is a natural flavonoid compound present in chamomile (Matricaia chamomilla L.) from the Asteraceae family, which is used in the treatment of cardiovascular diseases by traditional healers, but its effects on differentiation and extracellular matrix (ECM) production of cardiac fibroblasts (CFs) induced by transforming growth factor beta 1 (TGF-β1) are poorly understood.This study aimed to ...
DOI:: 10.1016/j.jep.2020.113195
: https://dx.doi.org/10.1016/j.jep.2020.113195

20. Fucoxanthin extracted from Laminaria Japonica inhibits metastasis and enhances the sensitivity of lung cancer to Gefitinib

Author:: Ming, Jia Xiong; Wang, Zhao Cong; Huang, Yi; Ohishi, Hifumi; Wu, Rong Ji; Shao, Yan; Wang, Hui; Qin, Ming Yang; Wu, Ze Liang; Li, Yi Yong; Chang Zhou, Shun; Chen, Hui; Liu, Hui; Xu, Rong
Source:: Journal of ethnopharmacology 2021 v.265 pp. 113302
ISSN:: 0378-8741
Subject:: Oriental traditional medicine; RNA interference; Saccharina japonica; Western blotting; antineoplastic activity; cadherins; cell lines; cell proliferation; cell viability; drugs; fibronectins; fluorescent antibody technique; gene expression; human diseases; immunohistochemistry; lung neoplasms; lungs; metastasis; models; neoplasm cells; phenotype; phosphatidylinositol 3-kinase; staining; tissue repair; transcription factor NF-kappa B; xenotransplantation
Abstract:: ... Laminaria japonica, a brown seaweed, has been used in Traditional Chinese Medicine (TCM) to treat a variety of diseases including lung cancer.To demonstrate the effects of Fucoxanthin (FX), a major active component extracted from Laminaria japonica on metastasis and Gefitinib (Gef) sensitivity in human lung cancer cells both in vitro and in vivo.Invasion and migration of lung cancer cells were det ...
DOI:: 10.1016/j.jep.2020.113302
: https://dx.doi.org/10.1016/j.jep.2020.113302

21. Myosin light chain kinase mediates intestinal barrier dysfunction following simulated microgravity based on proteomic strategy

Author:: Wang, Shibo; Wang, Rui; Li, George Q.; Cho, Jun-Lae; Deng, Yulin; Li, Yujuan
Source:: Journal of proteomics 2021 v.231 pp. 104001
ISSN:: 1874-3919
Subject:: Western blotting; adhesion; animal models; etiology; extracellular space; focal adhesions; gene expression regulation; goblet cells; histology; intestinal mucosa; microfilaments; microgravity; myosin; myosin light chain kinase; permeability; phosphorylation; protein synthesis; proteomics; rats; small intestine; therapeutics
Abstract:: ... Microgravity induces injury of intestinal barrier. However, the underlying mechanism remains unclear. The present study aimed to investigate the pathological change of intestinal mucosa induced by long term simulated microgravity and to explore its etiological mechanism using a proteomic approach. The well accepted tail-suspended rat model was used to simulate microgravity. The damage of rat small ...
DOI:: 10.1016/j.jprot.2020.104001
: https://dx.doi.org/10.1016/j.jprot.2020.104001

22. Quantitative proteomics of cerebrospinal fluid using tandem mass tags in dogs with recurrent epileptic seizures

Author:: Baka, Rania; Eckersall, David; Horvatic, Anita; Gelemanovic, Andrea; Mrljak, Vladimir; McLaughlin, Mark; Athanasiou, Labrini V.; Papaioannou, Nikolaos; Stylianaki, Ioanna; Hanh, Han Quang; Chadwick, Christopher C.; Polizopoulou, Zoe
Source:: Journal of proteomics 2021 v.231 pp. 103997
ISSN:: 1874-3919
Subject:: Canis lupus; Western blotting; blood-brain barrier; brain; cerebrospinal fluid; data analysis; dogs; drug therapy; epilepsy; gene ontology; immunoassays; prospective studies; proteins; proteome; proteomics; seizures; spectrometers
Abstract:: ... This prospective study included four dog groups (group A: healthy dogs, groups B: dogs with idiopathic epilepsy under antiepileptic medication (AEM), C: idiopathic epilepsy dogs without AEM administration, D: dogs with structural epilepsy). The purpose of the study was to compare the proteomic profile among the four groups. Samples were analyzed by a quantitative Tandem Mass Tags approach using a ...
DOI:: 10.1016/j.jprot.2020.103997
: https://dx.doi.org/10.1016/j.jprot.2020.103997

23. Saliva-omics in plasma cell disorders- Proof of concept and potential as a non-invasive tool for monitoring disease burden

Author:: Tierney, Ciara; Bazou, Despina; Lê, Giao; Dowling, Paul; O'Gorman, Peter
Source:: Journal of proteomics 2021 v.231 pp. 104015
ISSN:: 1874-3919
Subject:: B-lymphocytes; Western blotting; anemia; biomarkers; blood serum; burden of disease; disease progression; enzyme-linked immunosorbent assay; hypercalcemia; liquid chromatography; monitoring; myeloma; patients; precision medicine; protein synthesis; proteins; relapse; saliva; tandem mass spectrometry
Abstract:: ... Multiple Myeloma (MM), the second most common lymphoid cancer worldwide, is characterised by the uninhibited proliferation of terminally differentiated B-lymphocytes. Leading to The diagnosis typically requires the presence of a monoclonal protein (M protein) and the demonstration of CRAB features (hypercalcemia, renal impairment, anaemia and bone lesions). MM is considered incurable as, due to se ...
DOI:: 10.1016/j.jprot.2020.104015
: https://dx.doi.org/10.1016/j.jprot.2020.104015

24. Ameliorative effects of Qingganjiuwei powder, a traditional Mongolian medicine, against CCl4-induced liver fibrosis in rats

Author:: Ge, Hongyan; Wang, Anqing; Su, Ya; Yu, ChunLei; Gao, Lu; Li, Yuxin
Source:: Journal of ethnopharmacology 2021 v.264 pp. 113226
ISSN:: 0378-8741
Subject:: Western blotting; actin; alanine transaminase; aspartate transaminase; blood serum; carbon tetrachloride; collagen; drugs; enzyme activity; fibrosis; gelatinase A; gene expression regulation; hematologic tests; histopathology; immunohistochemistry; liver; liver cirrhosis; messenger RNA; mitogen-activated protein kinase; models; muscles; necrosis; phosphorylation; protein synthesis; quantitative polymerase chain reaction; rats; signal transduction; silymarin; traditional medicine; China
Abstract:: ... Qingganjiuwei powder (QGJWS) is a well-known traditional drug containing nine kinds of medicinal materials. This drug is commonly used in the Inner Mongolia region and exerts remarkable clinical effects on hepatic protection.To investigate whether QGJWS inhibits liver fibrosis in rats and to reveal its potential mechanisms.Liver fibrosis model was induced by CCl₄ for 8 weeks in SD rats. Next, rats ...
DOI:: 10.1016/j.jep.2020.113226
: https://dx.doi.org/10.1016/j.jep.2020.113226

25. Berberine inhibits intestinal epithelial barrier dysfunction in colon caused by peritoneal dialysis fluid by improving cell migration

Author:: Zhang, Dongliang; Jiang, Lan; Wang, Mengling; Jin, Meiping; Zhang, Xuemei; Liu, Difa; Wang, Zhangwei; Yang, Licai; Xu, Xudong
Source:: Journal of ethnopharmacology 2021 v.264 pp. 113206
ISSN:: 0378-8741
Subject:: Coptis chinensis; Oriental traditional medicine; Western blotting; adhesion; berberine; cell movement; colon; dialysis; diarrhea; epithelial cells; gastrointestinal diseases; herbal medicines; histology; intestinal mucosa; medicinal plants; mice; microvilli; models; plant extracts; protective effect; rats; renal failure; rhizomes; staining; therapeutics; tight junctions; tissue repair; transmission electron microscopy
Abstract:: ... Berberine is generally extracted from Rhizoma Coptidis (Coptis chinensis Franch), a traditional Chinese medicine, which can be used in the treatment of intestinal diseases, respiratory infections and cardiovascular diseases. Berberine is especially effective for the treatment of gastrointestinal disorders such as diarrhea because of the effect of heat-clearing and detoxifying in traditional Chines ...
DOI:: 10.1016/j.jep.2020.113206
: https://dx.doi.org/10.1016/j.jep.2020.113206

26. Effect of weimaining on apoptosis and Caspase-3 expression in a breast cancer mouse model

Author:: Ke, Haoliang; Wang, Xiuping; Zhou, Zhen; Ai, Wang; Wu, Zhaoyan; Zhang, Yingwen
Source:: Journal of ethnopharmacology 2021 v.264 pp. 113363
ISSN:: 0378-8741
Subject:: Fagopyrum dibotrys; Western blotting; animal models; antineoplastic activity; apoptosis; bioluminescence; breast neoplasms; breasts; buckwheat; caspase-3; gene expression; gene expression regulation; heparin; herbal medicines; image analysis; intraperitoneal injection; messenger RNA; mice; neoplasm cells; nucleotidyltransferases; photons; proanthocyanidins; protein content; reverse transcriptase polymerase chain reaction; roots; sodium chloride; traditional medicine; xenotransplantation
Abstract:: ... Weimaining (WMN) is a condensed Tannin compound extracted from Fagopyrum cymosum (Trevir.) Meisn., which comes from the roots of buckwheat, a type of Chinese herbal medicine, was first recorded in “Bencao Shiyi”. WMN has inhibitory effects on multiple cancer types and is widely used in clinical practice; however, the mechanism underlying the anti-tumor effect of WMN is still unclear.To investigate ...
DOI:: 10.1016/j.jep.2020.113363
: https://dx.doi.org/10.1016/j.jep.2020.113363

27. Ethanol extract of Liriodendron chinense (Hemsl.) Sarg barks attenuates hyperuricemic nephropathy by inhibiting renal fibrosis and inflammation in mice

Author:: Pan, Jing; Zhang, Chunle; Shi, Min; Guo, Fan; Liu, Jing; Li, Lingzhi; Ren, Qian; Tao, Sibei; Tang, Minghai; Ye, Haoyu; Ma, Liang; Fu, Ping
Source:: Journal of ethnopharmacology 2021 v.264 pp. 113278
ISSN:: 0378-8741
Subject:: ABC transporters; Liriodendron tulipifera; Oriental traditional medicine; Western blotting; adenine; animal models; blood serum; creatinine; ethanol; excretion; fibrosis; fluorescent antibody technique; gout; histochemistry; histopathology; inflammation; kidney diseases; kidneys; mice; mitogen-activated protein kinase; non-specific protein-tyrosine kinase; oral administration; potassium; protective effect; quantitative polymerase chain reaction; renal function; reverse transcriptase polymerase chain reaction; signal transduction; transcription factor NF-kappa B; tumor necrosis factor-alpha; urea nitrogen; uric acid; urine; China
Abstract:: ... Liriodendron chinense (Hemsl.) Sarg, known as the Chinese tulip tree, has a long history of cultivation and utilization in many Asia countries, especially in China to use in traditional Chinese medicine for expelling “wind and dampness”, a term corresponding to rheumatic fever and rheumatoid arthritis. Interestingly, the barks of Liriodendron chinense (Hemsl.) Sarg was also found in folk to treat ...
DOI:: 10.1016/j.jep.2020.113278
: https://dx.doi.org/10.1016/j.jep.2020.113278

28. Kanglaite enhances the efficacy of cisplatin in suppression of hepatocellular carcinoma via inhibiting CKLF1 mediated NF-κB pathway and regulating transporter mediated drug efflux

Author:: Chen, Chen; Ai, Qi-di; Wei, Yu-hui
Source:: Journal of ethnopharmacology 2021 v.264 pp. 113388
ISSN:: 0378-8741
Subject:: Coix lacryma-jobi; Western blotting; cell viability; cisplatin; drug therapy; fluorescent antibody technique; gene overexpression; genes; hepatoma; human cell lines; inflammation; interleukin-1beta; models; neoplasm cells; neoplasm progression; quantitative polymerase chain reaction; radiotherapy; staining; synergism; tissue repair; traditional medicine; transcription factor NF-kappa B; transporters; tumor necrosis factor-alpha
Abstract:: ... Kanglaite (KLT) is an active extract of the Coix lacryma-jobi seed, which can benefit Qi and nourish Yin, and disperse the accumulation of evils. It is used as a biphasic broad-spectrum anti-cancer drug, and shows synergistic effects with radiotherapy and chemotherapy. However, the mechanism of KLT combined with cisplatin (CDDP) against hepatocellular carcinoma (HCC) has not been elucidated.The ai ...
DOI:: 10.1016/j.jep.2020.113388
: https://dx.doi.org/10.1016/j.jep.2020.113388

29. Kuijieling decoction suppresses NLRP3-Mediated pyroptosis to alleviate inflammation and experimental colitis in vivo and in vitro

Author:: Jie, Fengming; Xiao, Suting; Qiao, Yang; You, Yihui; Feng, Yuan; Long, Yu; Li, Sixin; Wu, Yanli; Li, Yanwu; Du, Qun
Source:: Journal of ethnopharmacology 2021 v.264 pp. 113243
ISSN:: 0378-8741
Subject:: Western blotting; amino acid sequences; animal models; autoimmune diseases; blood serum; caspase-1; cell culture; colon; colorectal neoplasms; dextran sulfate; enzyme-linked immunosorbent assay; fluorescent antibody technique; immunohistochemistry; inflammation; interleukin-18; interleukin-1beta; messenger RNA; mice; morbidity; mortality; protein content; protein synthesis; pyroptosis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; staining; traditional medicine; ulcerative colitis
Abstract:: ... Ulcerative colitis (UC) is an autoimmune disease. Although the mortality rate of UC is not very high, it has a considerable morbidity rate and an unsatisfactory cure rate. Without effective treatment, UC is likely to develop into colon cancer. Kuijieling (KJL) is an effective empirical formula to treat UC in the clinical setting, and it has been proven to have curative effects against UC.In a prev ...
DOI:: 10.1016/j.jep.2020.113243
: https://dx.doi.org/10.1016/j.jep.2020.113243

30. Lophatherum gracile Brongn. attenuates neutrophilic inflammation through inhibition of JNK and calcium

Author:: Lai, Kuei-Hung; Chen, Po-Jen; Chen, Chih-Chuan; Yang, Sien-Hung; El-Shazly, Mohamed; Chang, Yu-Chia; Wu, Yi-Hsuan; Wu, Yi-Hsiu; Wang, Yi-Hsuan; Hsieh, Hsi-Lung; Hwang, Tsong-Long
Source:: Journal of ethnopharmacology 2021 v.264 pp. 113224
ISSN:: 0378-8741
Subject:: Western blotting; adhesion; anti-inflammatory activity; calcium; chemotaxis; elastase; enzyme inhibition; enzyme inhibitors; heat; herbal medicines; human cell lines; humans; inflammation; mitogen-activated protein kinase; neutrophils; non-specific serine/threonine protein kinase; phosphorylation; signal transduction; spectroscopy; superoxide anion; traditional medicine
Abstract:: ... Lophatherum gracile Brongn. (L. gracile) has been long used in traditional herbal medicine to clinically clear heat, disinhibit dampness, and treat inflammation. However, the effect of L. gracile on the activation of human neutrophils remains unclear.The aim of current study is to investigate the anti-inflammatory properties of L. gracile extract (LGE) in N-formyl-methionyl-leucyl-phenylalanine (f ...
DOI:: 10.1016/j.jep.2020.113224
: https://dx.doi.org/10.1016/j.jep.2020.113224

31. Repair function of essential oil from Crocodylus Siamensis (Schneider, 1801) on the burn wound healing via up-regulated growth factor expression and anti-inflammatory effect

Author:: Li, Hua-Liang; Liu, Xiao-Tian; Huang, Shao-Min; Xiong, You-Xiong; Zhang, Zi-Ran; Zheng, Ya-Hui; Chen, Qing-Xi; Chen, Qiong-Hua
Source:: Journal of ethnopharmacology 2021 v.264 pp. 113286
ISSN:: 0378-8741
Subject:: Crocodylus siamensis; Western blotting; anti-inflammatory activity; burns (injuries); collagen; crocodiles; dermis; enzyme-linked immunosorbent assay; essential oils; immunohistochemistry; interleukin-1beta; interleukin-6; melanin; messenger RNA; mitogen-activated protein kinase; nitric oxide; oils; phosphorylation; proliferating cell nuclear antigen; rats; staining; tissue repair; traditional medicine; transforming growth factor beta 1; transmission electron microscopy; tumor necrosis factor-alpha; ultrastructure
Abstract:: ... Crocodile oil has been used by traditional physicians around the world to treat wound healing and inflammation. However, the scientific rationale and mechanism behind its use in vivo has not been fully researched.We mainly investigated the mechanism during crocodile oil treatment of up-regulated growth factor expression and anti-inflammatory on burn wound healing in rats.The moisture and nitric ox ...
DOI:: 10.1016/j.jep.2020.113286
: https://dx.doi.org/10.1016/j.jep.2020.113286

32. San Wu Huangqin decoction regulates inflammation and immune dysfunction induced by influenza virus by regulating the NF-κB signaling pathway in H1N1-infected mice

Author:: Ma, Qin-hai; Ren, Meng-yue; Luo, Jia-bo
Source:: Journal of ethnopharmacology 2021 v.264 pp. 112800
ISSN:: 0378-8741
Subject:: B-lymphocytes; CD4-positive T-lymphocytes; IKappaB kinase; Influenza A virus; Rehmannia glutinosa; Scutellaria baicalensis; Sophora flavescens; Western blotting; animal models; blood serum; cytotoxicity; fever; immune response; immunohistochemistry; inflammation; interferon-gamma; interleukin-1beta; interleukin-4; interleukin-6; lungs; macrophages; mice; natural killer cells; protein synthesis; signal transduction; traditional medicine; transcription factor NF-kappa B; tubers; tumor necrosis factor-alpha
Abstract:: ... The San Wu Huangqin Decoction (SWHD), which is made from the dried root of Sophora flavescens Aiton (Kushen in Chinese), the dried root of Scutellaria baicalensis Georgi (Huangqin in Chinese), and the dried root tuber of Rehmannia glutinosa (Gaertn.) DC. (Dihuang in Chinese), is a traditional Chinese formula used to treat prolonged fever and inflammatory diseases in clinics and proven to inhibit i ...
DOI:: 10.1016/j.jep.2020.112800
: https://dx.doi.org/10.1016/j.jep.2020.112800

33. TMT-based proteomics analysis reveals the efficacy of jiangzhuo formula in improving the lipid profiles of dyslipidemia rats

Author:: Pan, Linlin; Tian, Yuan; Sun, Haiyang; Wang, Yao; Liu, Guirong
Source:: Journal of ethnopharmacology 2021 v.264 pp. 113390
ISSN:: 0378-8741
Subject:: Western blotting; apolipoprotein B; apolipoprotein E; bile secretion; blood lipids; blood serum; cholesterol; cholesterol metabolism; drugs; gene expression regulation; gene ontology; genes; high fat diet; hydroxymethylglutaryl-CoA synthase; hyperlipidemia; lipid composition; liquid chromatography; liver; mass spectrometry; peroxisome proliferator-activated receptors; polymerase chain reaction; protein synthesis; protein-protein interactions; proteomics; rats; signal transduction; staining; traditional medicine
Abstract:: ... Jiangzhuo Formula (JZF) is a traditional Chinese herbal prescription that is clinically applied to treat dyslipidemia. However, the mechanism underlying its efficacy remains unexplored.This study aims to elucidate the underlying mechanisms, explore potential pathways, and identify the key proteins of JZF for the treatment of dyslipidemia.In this work, Q-Orbitrap high-resolution liquid chromatograp ...
DOI:: 10.1016/j.jep.2020.113390
: https://dx.doi.org/10.1016/j.jep.2020.113390

34. Xiaoyaosan decoction alleviated rat liver fibrosis via the TGFβ/Smad and Akt/FoxO3 signaling pathways based on network pharmacology analysis

Author:: Zhou, Yuan; Wu, Rong; Cai, Fei-Fei; Zhou, Wen-Jun; Lu, Yi-Yu; Zhang, Hui; Chen, Qi-Long; Su, Shi-Bing
Source:: Journal of ethnopharmacology 2021 v.264 pp. 113021
ISSN:: 0378-8741
Subject:: Oriental traditional medicine; Western blotting; blood serum; carbon tetrachloride; collagen; fibrosis; genes; hepatitis B; hepatoma; herbs; histopathology; liver; liver cirrhosis; liver failure; liver function; mitogen-activated protein kinase; models; peroxisome proliferator-activated receptors; pharmacology; phosphorylation; rats; signal transduction; transcriptomics; transforming growth factor beta 1
Abstract:: ... Liver fibrosis is an outcome of many chronic liver diseases and often results in cirrhosis, liver failure, and even hepatocarcinoma. Xiaoyaosan decoction (XYS) as a classical Traditional Chinese Medicine (TCM) formula is used to liver fibrosis in clinical practice while its mechanism is unclear.The aim of this study was to investigate the anti-fibrosis effect of XYS and to explore the molecular me ...
DOI:: 10.1016/j.jep.2020.113021
: https://dx.doi.org/10.1016/j.jep.2020.113021

35. Proteomic analysis of bEnd.3 cells infected with wild-type and stk-deficient strains of Streptococcus suis serotype 2 reveals protein and pathway regulation

Author:: Li, Weiyi; Yin, Yifan; Meng, Yu; Zhou, Hong; Ma, Zhe; Lin, Huixing; Fan, Hongjie
Source:: Journal of proteomics 2021 v.230 pp. 103983
ISSN:: 1874-3919
Subject:: RNA; Streptococcus suis; Western blotting; bacteria; blood-brain barrier; cytoskeleton; gene expression regulation; genes; human diseases; immune response; meningitis; models; pathogens; protein synthesis; protein-serine-threonine kinases; proteins; proteomics; quantitative polymerase chain reaction; serine; serotypes; swine; threonine; tight junctions; transcription (genetics)
Abstract:: ... Streptococcus suis serotype 2 (SS2) is a zoonotic pathogen causing meningitis in humans and pigs. However, information on the comparative protein expression of the blood-brain barrier (BBB) following SS2 infection is limited. Deletion of the serine/threonine kinase (stk) gene can decrease the ability of SS2 to invade the BBB. In the present study, bEnd.3 cells were used as the BBB model, and a SIL ...
DOI:: 10.1016/j.jprot.2020.103983
: https://dx.doi.org/10.1016/j.jprot.2020.103983

36. Diallyl disulfide improves lipid metabolism by inhibiting PCSK9 expression and increasing LDL uptake via PI3K/Akt-SREBP2 pathway in HepG2 cells

Author:: Wu, Ya-Ru; Li, Liang; Sun, Xian-Chang; Wang, Jun; Ma, Chun-Yan; Zhang, Yue; Qu, Hui-Lin; Xu, Rui-Xia; Li, Jian-Jun
Source:: Nutrition, metabolism, and cardiovascular diseases 2021 v.31 no.1 pp. 322-332
ISSN:: 0939-4753
Subject:: Western blotting; assays; atorvastatin; cardiovascular diseases; diallyl disulfide; dose response; drugs; garlic; human cell lines; lipid metabolism; lipopolysaccharides; nutrition; quantitative polymerase chain reaction; signal transduction; sulfides
Abstract:: ... Diallyl disulfide (DADS), a volatile sulfide extracted from garlic, has been suggested as a chemical of anti-atherosclerotic drugs, while its molecular mechanism for this benefit has not fully been understood. The aim of the present study was to investigate the effects of DADS on lipid metabolism and its potential mechanisms in HepG2 cells induced by lipopolysaccharides (LPS).HepG2 cells were trea ...
DOI:: 10.1016/j.numecd.2020.08.012
: https://dx.doi.org/10.1016/j.numecd.2020.08.012

37. An ecofriendly synthesized gold nanoparticles induces cytotoxicity via apoptosis in HepG2 cells

Author:: Nandhini, Joseph T.; Ezhilarasan, Devaraj; Rajeshkumar, Shanmugam
Source:: Environmental toxicology 2021 v.36 no.1 pp. 24-32
ISSN:: 1520-4081
Subject:: Enterococcus; Western blotting; apoptosis; assays; cytochrome c; cytosol; cytotoxicity; ecotoxicology; hepatoma; human cell lines; humans; microorganisms; mitochondria; mortality; nanogold; nanoparticles; neoplasm cells; proliferating cell nuclear antigen; propidium; protein synthesis; reactive oxygen species; staining; synthesis
Abstract:: ... Microbes have long been used for the synthesis of a variety of nanoparticles. Hepatocellular carcinoma (HCC) is the primary liver cancer and it is the second leading cause of cancer‐related mortality worldwide. In this study, we have synthesized Enterococcus mediated gold nanoparticles (AuNPs) and investigated their cytotoxic potential against human hepatocellular cancer cell line (HepG2). AuNPs w ...
DOI:: 10.1002/tox.23007
: http://dx.doi.org/10.1002/tox.23007

38. Cardiac glycosides from the roots of Streblus asper Lour. and their apoptosis-inducing activities in A549 cells

Author:: Zhang, Bo-dou; Zhu, Wan-fang; Akihisa, Toshihiro; Kikuchi, Takashi; Ukiya, Motohiko; Maya, Fukuda; Xu, Jian; Liu, Wen-yuan; Feng, Feng; Zhang, Jie
Source:: Phytochemistry 2021 v.181 pp. 112544
ISSN:: 0031-9422
Subject:: Streblus asper; Western blotting; antineoplastic activity; apoptosis; cardiac glycosides; caspase-3; caspase-8; caspase-9; cell proliferation; cytotoxicity; enzyme activation; inhibitory concentration 50; lung neoplasms; mass spectrometry; neoplasm cells; nuclear magnetic resonance spectroscopy; phytopharmaceuticals; roots
Abstract:: ... Phytochemical investigation of the roots of Streblus asper Lour. resulted in the isolation of six previously undescribed cardiac glycosides, designated 2′-de-O-methylstrebloside (1), cannogenol-3α-O-β-D-gluopyranosyl-(1 → 4)-6-deoxy −2,3-dimethoxyl-β-D-fucopyranoside (2), periplogenin-3-O-α-L-rhamnopyranosyl -(1 → 4)-6-deoxy-β-D-allopyranoside (3), 5-de-O-hydroxylstrebloside (4), 5βH-16β-hydroxylk ...
DOI:: 10.1016/j.phytochem.2020.112544
: https://dx.doi.org/10.1016/j.phytochem.2020.112544

39. D‐galactose‐induced toxicity associated senescence mitigated by alpinate oxyphyllae fructus fortified adipose‐derived mesenchymal stem cells

Author:: Lin, Hung‐Jen; Ramesh, Samiraj; Chang, Yung‐Ming; Tsai, Chuan‐Te; Tsai, Chin‐Chuan; Shibu, Marthandam Asokan; Tamilselvi, Shanmugam; Mahalakshmi, B.; Kuo, Wei‐Wen; Huang, Chih‐Yang
Source:: Environmental toxicology 2021 v.36 no.1 pp. 86-94
ISSN:: 1520-4081
Subject:: Alpinia oxyphylla; Western blotting; collagen; ecotoxicology; eosin; extracts; fibrosis; heart; histopathology; hypertrophy; immunohistochemistry; interleukin-6; males; mesenchymal stromal cells; oxidative stress; rats; staining; stem cells; toxicity
Abstract:: ... This study addresses the effect of D‐galactose‐induced toxicity associated senescence mitigated by alpinate oxyphyllae fructus (AOF; Alpinia oxyphylla Miq) extracts fortified with adipose‐derived mesenchymal stem cells (ADMSCs) in rats. Male 18 week‐old Wistar Kyoto (WKY) rats were used in this study. We analyzed cardiac fibrosis by Masson's trichrome staining. The tissue sections were dyed using ...
DOI:: 10.1002/tox.23014
: http://dx.doi.org/10.1002/tox.23014

40. Prolactin neuroprotective action against excitotoxic insult in the hippocampus of male mice

Author:: Anagnostou, Ilektra; Muñoz-Mayorga, Daniel; Morales, Teresa
Source:: Peptides 2021 v.135 pp. 170425
ISSN:: 0196-9781
Subject:: Western blotting; adults; cell death; cell viability; females; hippocampus; immunohistochemistry; males; mice; mitogen-activated protein kinase; neurodegenerative diseases; neurons; neuroprotective effect; prolactin; protein content; rats
Abstract:: ... Prolactin (PRL) is known to exert neuroprotective effects against excitotoxic damage in the hippocampus of female rats, both in vitro and in vivo. It is still unknown whether this effect can be seen in the male hippocampus and intracellular signaling mediating such action. To assess this, adult male CD-1 mice were subjected to excitotoxic damage with kainic acid (KA; i.c.v.), after a) no manipulat ...
DOI:: 10.1016/j.peptides.2020.170425
: https://dx.doi.org/10.1016/j.peptides.2020.170425

41. Rumen-bypassed tributyrin alleviates heat stress by reducing the inflammatory responses of immune cells

Author:: Guo, Wenjin; Liu, Juxiong; Yang, Yuanxi; Ma, He; Gong, Qian; Kan, Xingchi; Ran, Xin; Cao, Yu; Wang, Jianfa; Fu, Shoupeng; Hu, Guiqiu
Source:: Poultry science 2021 v.100 no.1 pp. 348-356
ISSN:: 0032-5791
Subject:: Western blotting; albumins; aspartate transaminase; dairy cows; enzyme inhibition; globulins; heat stress; hematologic tests; hemoglobin; humidity; inflammation; interleukin-1beta; interleukin-6; lymphocytes; mitogen-activated protein kinase; quantitative polymerase chain reaction; respiratory rate; reverse transcriptase polymerase chain reaction; signal transduction; summer; temperature; tributyrin; tumor necrosis factor-alpha
Abstract:: ... Heat stress (HS) in summer will seriously affect the health and performance of dairy cows. To alleviate the injury to dairy cows caused by HS, we added the rumen-bypassed tributyrin to the feed. We determined whether cows were in a heat-stressed environment by testing the temperature humidity index in the morning, at noon, and in the evening. The detection of anal temperature and respiratory frequ ...
DOI:: 10.1016/j.psj.2020.10.006
: https://dx.doi.org/10.1016/j.psj.2020.10.006

42. The entomotoxin Jack Bean Urease changes cathepsin D activity in nymphs of the hematophagous insect Dipetalogaster maxima (Hemiptera: Reduviidae)

Author:: Moyetta, Natalia R.; Fruttero, Leonardo L.; Leyria, Jimena; Ramos, Fabian O.; Carlini, Célia R.; Canavoso, Lilián
Source:: Comparative biochemistry and physiology 2021 v.251 pp. 110511
ISSN:: 1096-4959
Subject:: Canavalia ensiformis; Dipetalogaster; Western blotting; catalytic activity; cathepsin D; enzyme activity; fat body; fluorescent antibody technique; hematophagous insects; hemocytes; in vitro studies; messenger RNA; midgut; models; nymphs; protein content; reproduction; seeds; urease
Abstract:: ... In insects, cathepsin D is a lysosomal aspartic endopeptidase involved in several functions such as digestion, defense and reproduction. Jack Bean Urease (JBU) is the most abundant urease isoform obtained from the seeds of the plant Canavalia ensiformis. JBU is a multifunctional protein with entomotoxic effects unrelated to its catalytic activity, by mechanisms not yet fully understood. In this wo ...
DOI:: 10.1016/j.cbpb.2020.110511
: https://dx.doi.org/10.1016/j.cbpb.2020.110511

43. The p53/RMRP/miR122 signaling loop promotes epithelial-mesenchymal transition during the development of silica-induced lung fibrosis by activating the notch pathway

Author:: Huang, Ruixue; Bai, Chenjun; Liu, Xiaodan; Zhou, Yao; Hu, Sai; Li, Decheng; Xiang, Jing; Chen, Jihua; Zhou, Pingkun
Source:: Chemosphere 2021 v.263 pp. 128133
ISSN:: 0045-6535
Subject:: Western blotting; assays; chromatin immunoprecipitation; fibrosis; lungs; microarray technology; mitochondrial RNA; non-coding RNA; quantitative polymerase chain reaction; reporter genes; silica; therapeutics
Abstract:: ... Understanding the roles of long noncoding RNAs (lncRNAs) in EMT would help with establishing novel avenues for further uncovering the mechanisms of lung fibrosis and identifying preventative and therapeutic targets. This study aimed to identify silica-induced specific lncRNAs and investigate the feedback loop regulation among their upstream and downstream genes.A microarray assay, quantitative rea ...
DOI:: 10.1016/j.chemosphere.2020.128133
: https://dx.doi.org/10.1016/j.chemosphere.2020.128133

44. The relationship between the mTOR signaling pathway and ovarian aging in peak-phase and late-phase laying hens

Author:: Hao, Er-Ying; Wang, De-He; Chen, Yi-Fan; Zhou, Rong-Yan; Chen, Hui; Huang, Ren-Lu
Source:: Poultry science 2021 v.100 no.1 pp. 334-347
ISSN:: 0032-5791
Subject:: Western blotting; agonists; antioxidants; apoptosis; developmental stages; egg production; egg quality; enzyme-linked immunosorbent assay; follicular development; gene expression; genes; glutathione peroxidase; granulosa cells; immunoglobulin G; immunoglobulin M; laying hens; messenger RNA; protein kinase C; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction; superoxide dismutase
Abstract:: ... The molecular mechanisms underlying reproductive aging in avian species are poorly understood. Previous studies have shown the importance of mechanistic target of rapamycin (mTOR) signaling pathway in aging. In this study, we investigated the relationship between the mTOR signaling pathway and ovarian aging in the peak phase and late phase of egg production in laying hens. The egg production rate ...
DOI:: 10.1016/j.psj.2020.10.005
: https://dx.doi.org/10.1016/j.psj.2020.10.005

45. Chronic ethanol exposure induces neuroinflammation in H4 cells through TLR3 / NF-κB pathway and anxiety-like behavior in male C57BL/6 mice

Author:: Wang, Xiaolong; Yu, Hao; Wang, Changliang; Liu, Yang; You, Jiabin; Wang, Pengfei; Xu, Guohui; Shen, Hui; Yao, Hui; Lan, Xinze; Zhao, Rui; Wu, Xu; Zhang, Guohua
Source:: Toxicology 2020 v.446 pp. 152625
ISSN:: 0300-483X
Subject:: RNA interference; Toll-like receptor 3; Toll-like receptor 4; Western blotting; adults; alcohol abuse; ammonium; animal experimentation; cell culture; culture media; drinking; elevated plus-maze test; enzyme-linked immunosorbent assay; ethanol; genes; glioma; hippocampus; human diseases; interleukin-1beta; interleukin-6; males; mice; neoplasm cells; public health; transcription factor NF-kappa B; tumor necrosis factor-alpha
Abstract:: ... Chronic alcoholism has become a major public health problem. Long-term and excessive drinking can lead to a variety of diseases. Chronic ethanol exposure can induce neuroinflammation and anxiety-like behavior, and this may be induced through the Toll-like receptor 3/nuclear factor-κB (TLR3/NF-κB) pathway. Animal experiments were performed using healthy adult male C57BL/6 N mice given 10 % (m/V) or ...
DOI:: 10.1016/j.tox.2020.152625
: https://dx.doi.org/10.1016/j.tox.2020.152625

46. Quantitative comparative analysis uncovered the role of E2 in Nile tilapia GSD + TE

Author:: Teng, Jian; Chen, Hong Ju; Xu, Gao Peng; Wang, Yao Yao; Zhao, Yan; Ji, Xiang Shan
Source:: Aquaculture 2020 v.529 pp. 735656
ISSN:: 0044-8486
Subject:: Oreochromis niloticus; Western blotting; aromatase; enzyme activity; estradiol; females; fish; gene expression regulation; genes; heat treatment; larvae; males; masculinization; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; sex determination; sex ratio; sex reversal; temperature; transcription factors
Abstract:: ... Nile tilapia is a GSD + TE (genotypic sex determination plus temperature effect) fish species. However, the role of 17β-estradiol (E2) during high-temperature-induced masculinization is unknown in fish species exhibiting GSD + TE. Because letrozole can specifically downregulate E2, the differences of the E2 level and sex ratio between the high-temperature-treated groups and various doses of letroz ...
DOI:: 10.1016/j.aquaculture.2020.735656
: https://dx.doi.org/10.1016/j.aquaculture.2020.735656

47. Phoenix dactylifera L. Seed Extract Exhibits Antioxidant Effects and Attenuates Melanogenesis in B16F10 Murine Melanoma Cells by Downregulating PKA Signaling

Author:: Huang, Huey-Chun; Wang, Shr-Shiuan; Tsai, Tsang-Chi; Ko, Wang-Ping; Chang, Tsong-Min
Source:: Antioxidants 2020 v.9 no.12
ISSN:: 2076-3921
Subject:: 2,2-diphenyl-1-picrylhydrazyl; Phoenix dactylifera; Western blotting; alpha-melanocyte-stimulating hormone; antioxidant activity; assays; cAMP-dependent protein kinase; cells; exhibitions; extraction; iron; mechanism of action; melanin; melanogenesis; melanoma; mice; mushrooms; reactive oxygen species; seed extracts; seeds; signal transduction; sulfonic acids; transcription factors; ultrasonics
Abstract:: ... Background: The mode of action of Phoenix dactylifera seed extract in skin care has never been explored. Methods: P. dactylifera L. seeds were extracted by ultrasonic extraction. The antioxidant characteristics of the extract were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) (ABTS⁺) assays and scavenging methods. The total phenolic cont ...
DOI:: 10.3390/antiox9121270
: http://dx.doi.org/10.3390/antiox9121270

48. Unconventional Secretion of Nigerolysins A from Aspergillus Species

Author:: Kraševec, Nada; Novak, Maruša; Barat, Simona; Skočaj, Matej; Sepčić, Kristina; Anderluh, Gregor
Source:: Microorganisms 2020 v.8 no.12
ISSN:: 2076-2607
Subject:: Aspergillus niger; Pleurotus ostreatus; Western blotting; bioinformatics; cytoplasm; fungi; hyphae; image analysis; immunocytochemistry; lipids; loci; phylogeny; prediction; protein secretion; proteins; Niger
Abstract:: ... Aegerolysins are small lipid-binding proteins particularly abundant in fungi. Aegerolysins from oyster mushrooms interact with an insect-specific membrane lipid and, together with MACPF proteins produced by the same organism, form pesticidal pore-forming complexes. The specific interaction with the same membrane lipid was recently demonstrated for nigerolysin A2 (NigA2), an aegerolysin from Asperg ...
DOI:: 10.3390/microorganisms8121973
: http://dx.doi.org/10.3390/microorganisms8121973

49. Fluorescence Detection of Type III Secretion Using a Glu-CyFur Reporter System in Citrobacter rodentium

Author:: Pendergrass, Heather A.; Johnson, Adam L.; Hotinger, Julia A.; May, Aaron E.
Source:: Microorganisms 2020 v.8 no.12
ISSN:: 2076-2607
Subject:: Citrobacter rodentium; Western blotting; animal models; assays; bacteria; cell culture; cells; detection; diarrhea; ellagic acid; enteropathogenic Escherichia coli; epigallocatechin gallate; fluorescence; infection; laboratories; mammals; quorum sensing; rapid methods; secretion; tannins; therapeutics; type III secretion system; virulence
Abstract:: ... Enteropathogenic Escherichia coli (EPEC) is a major cause of infantile diarrhea worldwide. EPEC and the closely related murine model of EPEC infection, Citrobacter rodentium, utilize a type III secretion system (T3SS) to propagate the infection. Since the T3SS is not essential for the bacteria to survive or propagate, inhibiting the virulence factor with a therapeutic would treat the infection wit ...
DOI:: 10.3390/microorganisms8121953
: http://dx.doi.org/10.3390/microorganisms8121953

50. An isoflavone extract from soybean cake suppresses 2,4-dinitrochlorobenzene-induced contact dermatitis

Author:: Chu, Thomas; Wu, Nan-Lin; Hsiao, Chien-Yu; Li, Hsin-Ju; Lin, Tung-Yi; Ku, Cheng-Hung; Hung, Chi-Feng
Source:: Journal of ethnopharmacology 2020 v.263 pp. 113037
ISSN:: 0378-8741
Subject:: IKappaB kinase; Western blotting; animal models; cell viability; chronic diseases; clinical trials; contact dermatitis; enzyme-linked immunosorbent assay; erythema; gene expression; in vitro studies; in vivo studies; inflammation; interleukin-8; isoflavones; keratinocytes; leukocytes; messenger RNA; mice; mitogen-activated protein kinase; oral administration; phosphorylation; photography; powders; protective effect; reverse transcriptase polymerase chain reaction; soybean cake; toxicity testing; traditional medicine
Abstract:: ... Numerous epidemiological and clinical studies have demonstrated the protective role of dietary isoflavones against development of several chronic diseases. ISO-1, one fraction of isoflavone powders derived from soybean cake, is reported to attenuate inflammation and photodamage.Contact dermatitis is a common inflammatory skin disease, which accounts for most occupational skin disorders. Instead of ...
DOI:: 10.1016/j.jep.2020.113037
: https://dx.doi.org/10.1016/j.jep.2020.113037

51. Danlou tablet inhibits the inflammatory reaction of high-fat diet-induced atherosclerosis in ApoE knockout mice with myocardial ischemia via the NF-κB signaling pathway

Author:: Gao, Shan; Xue, Xiaoxue; Yin, Jia; Gao, Lina; Li, Zhu; Li, Lin; Gao, Shuming; Wang, Shuo; Liang, Ru; Xu, Yilan; Yu, Chunquan; Zhu, Yan
Source:: Journal of ethnopharmacology 2020 v.263 pp. 113158
ISSN:: 0378-8741
Subject:: Oriental traditional medicine; Western blotting; animal models; anti-inflammatory activity; apolipoprotein E; atherosclerosis; coronary artery disease; echocardiography; enzyme-linked immunosorbent assay; eosin; herbal medicines; high fat diet; histopathology; hypoxia; immunohistochemistry; interleukin-1beta; interleukin-6; interleukin-8; isoprenaline; knockout mutants; medicinal plants; mice; necrosis; patients; protein synthesis; signal transduction; staining; therapeutics; transcription factor NF-kappa B; tumor necrosis factor-alpha; China
Abstract:: ... Danlou tablet (DLT), a traditional herbal formula, has been used to treat chest discomfort (coronary atherosclerosis) in China. Although the anti-inflammatory activities of DLT have been proposed previously, the mechanisms of DLT in treating atherosclerosis with myocardial ischemia (AWMI) remain unknown.Atherosclerosis can result in heart disease caused by stenosis or occlusion of the lumen, resul ...
DOI:: 10.1016/j.jep.2020.113158
: https://dx.doi.org/10.1016/j.jep.2020.113158

52. Holistic quality evaluation of Qingwen Baidu Decoction and its anti-inflammatory effects

Author:: Zheng, Yuanru; Liu, Shanhong; Fan, Chunlin; Zeng, Huhu; Huang, Hefei; Tian, Chunyang; Lu, Zibin; Cao, Huihui; Liu, Junshan; Yu, Linzhong
Source:: Journal of ethnopharmacology 2020 v.263 pp. 113145
ISSN:: 0378-8741
Subject:: Danio rerio; Oriental traditional medicine; Western blotting; anti-inflammatory activity; baicalin; chemometrics; cluster analysis; diodes; dose response; herbs; high performance liquid chromatography; macrophages; mechanism of action; models; monitoring; neutrophils; principal component analysis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction; staining; transcription factor NF-kappa B
Abstract:: ... Qingwen Baidu Decoction (QBD), a famous traditional Chinese medicine prescription with heat-clearing and detoxifying efficacies, is widely used in the treatment of inflammatory diseases. However, due to lack of holistic quality evaluation research, the further study on the detailed molecular mechanisms of action are still insufficient.This study aimed to evaluate the overall quality of QBD and to ...
DOI:: 10.1016/j.jep.2020.113145
: https://dx.doi.org/10.1016/j.jep.2020.113145

53. In vitro cultured calculus bovis attenuates cerebral ischaemia-reperfusion injury by inhibiting neuronal apoptosis and protecting mitochondrial function in rats

Author:: Lu, Feibiao; Wang, Lingfeng; Chen, Yanyue; Zhong, Xiaoming; Huang, Zhen
Source:: Journal of ethnopharmacology 2020 v.263 pp. 113168
ISSN:: 0378-8741
Subject:: Alzheimer disease; Western blotting; apoptosis; brain; caspase-3; caspase-9; cattle; cholelithiasis; cortex; edema; encephalopathy; eosin; histopathology; in vitro culture; infarction; mitochondria; neurons; protein content; proteins; rats; signal transduction; staining; stroke; traditional medicine; transmission electron microscopy; ultrastructure
Abstract:: ... In vitro cultured calculus bovis (ICCB), which is produced based on the formation mechanism of bovine gallstones, is used to replace the natural bezoar. It has been used in the clinic to treat brain diseases, including stroke, Alzheimer's disease and depression.ICCB is used to treat encephalopathy in the clinic. We explored the effects of ICCB on cerebral ischaemia-reperfusion injury (CIRI) and th ...
DOI:: 10.1016/j.jep.2020.113168
: https://dx.doi.org/10.1016/j.jep.2020.113168

54. Pharmacoproteomics reveals the mechanism of Chinese dragon's blood in regulating the RSK/TSC2/mTOR/ribosome pathway in alleviation of DSS-induced acute ulcerative colitis

Author:: Lin, Yilong; Xiong, Wanna; Xiao, Simeng; Li, Fang; Lu, Zhuo; Yan, Jiongyi; Fang, Xuewan; Cui, Xiaojuan; Wen, Yilei; Liang, Jianqin; Yu, Kai
Source:: Journal of ethnopharmacology 2020 v.263 pp. 113221
ISSN:: 0378-8741
Subject:: Dracaena cochinchinensis; Western blotting; animal models; bioinformatics; dextran sulfate; drinking water; gene expression regulation; histopathology; immunohistochemistry; intestines; liquid chromatography; mice; protein synthesis; proteins; proteomics; rapamycin; ribosomes; tandem mass spectrometry; traditional medicine; ulcerative colitis
Abstract:: ... Chinese dragon's blood (CDB), a crude drug extracted from Dracaena cochinchinensis (Lour.) S.C. Chen, has been historically applied for the treatment of various diseases, including ulcerative colitis (UC). Unfortunately, the underlying molecular mechanism remains unclear.In this paper, the effects of CDB treatment on a mouse model of acute UC and proteomic variation in colonic tissue were investig ...
DOI:: 10.1016/j.jep.2020.113221
: https://dx.doi.org/10.1016/j.jep.2020.113221

55. Shenqi Yizhi granules protect hippocampus of AD transgenic mice by modulating on multiple pathological processes

Author:: Ren, Jianting; Wei, Dongfeng; An, Haiting; Zhang, Junying; Zhang, Zhanjun
Source:: Journal of ethnopharmacology 2020 v.263 pp. 112869
ISSN:: 0378-8741
Subject:: Alzheimer disease; Astragalus membranaceus; DNA repair; Panax ginseng; Scutellaria baicalensis; Western blotting; amino acid metabolism; cognition; cognitive disorders; cytoskeleton; energy metabolism; fluorescence; fluorescent antibody technique; granules; herbal medicines; herbs; hippocampus; malate dehydrogenase; medicinal plants; mice; neurons; neuroplasticity; neuroprotective effect; patients; proteins; pyruvate kinase; signal transduction; stress response; synaptic transmission; therapeutics; traditional medicine; transgenic animals
Abstract:: ... Chinese herbal medicine (CHM) draws more attention to explore effective therapeutic strategy for Alzheimer's disease (AD). CHM usually uses combinations of herbs or herbal ingredients to treat diseases, with the components targeting different disease processes. CHM might improve cognition in AD and MCI patients by optimizing network activity, promoting neural plasticity and repairing damaged neuro ...
DOI:: 10.1016/j.jep.2020.112869
: https://dx.doi.org/10.1016/j.jep.2020.112869

56. Systematic biological and proteomics strategies to explore the regulation mechanism of Shoutai Wan on recurrent spontaneous Abortion's biological network

Author:: Zeng, Liuting; Yang, Kailin; Liu, Liting; Zhang, Tianqing; Liu, Huiping; Tan, Zhanwang; Lei, Lei
Source:: Journal of ethnopharmacology 2020 v.263 pp. 113156
ISSN:: 0378-8741
Subject:: Western blotting; abortion (animals); animal experimentation; bioinformatics; clinical trials; computer software; desorption; drugs; embryonic mortality; gene expression regulation; immunohistochemistry; matrix-assisted laser desorption-ionization mass spectrometry; mice; models; pH; peptides; phosphopyruvate hydratase; pregnancy; protein content; proteomics; signal transduction; therapeutics; traditional medicine; transferrin
Abstract:: ... Shoutai Wan (STW) is a classic herbal formula for the treatment of recurrent spontaneous abortion (RSA), and clinical studies have shown the effectiveness of STW on RSA. However, the molecular mechanism of STW treatment of RSA is still unclear.(1) Animal experiments: The normal pregnancy model was established with CBA/J*BALB/C, and the RSA model was established by CBA/J*DBA/2. The RSA model CBA/J* ...
DOI:: 10.1016/j.jep.2020.113156
: https://dx.doi.org/10.1016/j.jep.2020.113156

57. Wild simulated ginseng activates mouse macrophage, RAW264.7 cells through TRL2/4-dependent activation of MAPK, NF-κB and PI3K/AKT pathways

Author:: Um, Yurry; Eo, Hyun Ji; Kim, Hyun Jun; Kim, Kiyoon; Jeon, Kwon Seok; Jeong, Jin Boo
Source:: Journal of ethnopharmacology 2020 v.263 pp. 113218
ISSN:: 0378-8741
Subject:: Panax ginseng; Toll-like receptor 2; Toll-like receptor 4; Western blotting; herbal medicines; immunomodulators; inducible nitric oxide synthase; interleukin-1beta; interleukin-6; macrophage activation; macrophages; messenger RNA; mice; mitogen-activated protein kinase; nitric oxide; phagocytosis; phosphatidylinositol 3-kinase; reverse transcriptase polymerase chain reaction; signal transduction; toxicity testing; traditional medicine; transcription factor NF-kappa B; tumor necrosis factor-alpha; viability
Abstract:: ... Ginseng (Panax ginseng Meyer) is a very well-known traditional herbal medicine that has long been used to enhance the body's immunity. Because it is a type of ginseng, it is believed that wild simulated ginseng (WSG) also has immune-enhancing activity. However, study on the immune-enhancing activity of WSG is quite insufficient compared to ginseng.In this study, we evaluated immune-enhancing activ ...
DOI:: 10.1016/j.jep.2020.113218
: https://dx.doi.org/10.1016/j.jep.2020.113218

58. A practical guide for analysis of histone post-translational modifications by mass spectrometry: Best practices and pitfalls

Author:: Thomas, Sydney P.; Haws, Spencer A.; Borth, Laura E.; Denu, John M.
Source:: Methods 2020 v.184 pp. 53-60
ISSN:: 1046-2023
Subject:: Western blotting; data quality; environmental factors; epigenetics; histone code; histones; human cell lines; mass spectrometry; post-translational modification; proteomics; statistics
Abstract:: ... Advances in mass spectrometry (MS) have revolutionized the ability to measure global changes in histone post-translational modifications (PTMs). The method routinely quantifies over 60 modification states in a single sample, far exceeding the capabilities of traditional western blotting. Thus, MS-based histone analysis has become an increasingly popular tool for understanding how genetic and envir ...
DOI:: 10.1016/j.ymeth.2019.12.001
: https://dx.doi.org/10.1016/j.ymeth.2019.12.001

59. Modulation of peroxynitrite produced via mitochondrial nitric oxide synthesis during Ca2+ and succinate-induced oxidative stress in cardiac isolated mitochondria

Author:: Gerdes, Harrison J.; Yang, Meiying; Heisner, James S.; Camara, Amadou K.S.; Stowe, David F.
Source:: Biochimica et biophysica acta 2020 v.1861 no.12 pp. 148290
ISSN:: 0005-2728
Subject:: Western blotting; buffers; calcium; calcium chloride; cardiomyocytes; electron microscopy; fluorescence; guinea pigs; hydrogen peroxide; inducible nitric oxide synthase; liver; menadione; mitochondria; neuronal nitric oxide synthase; nitric oxide; oxidants; oxidation; oxidative stress; pH; succinic acid; superoxide anion; superoxide dismutase; tyrosine
Abstract:: ... We hypothesized that NO• is generated in isolated cardiac mitochondria as the source for ONOO⁻ production during oxidative stress. We monitored generation of ONOO⁻ from guinea pig isolated cardiac mitochondria subjected to excess Ca²⁺ uptake before adding succinate and determined if ONOO⁻ production was dependent on a nitric oxide synthase (NOS) located in cardiac mitochondria (mtNOS). Mitochondri ...
DOI:: 10.1016/j.bbabio.2020.148290
: https://dx.doi.org/10.1016/j.bbabio.2020.148290

60. Study on the attenuated effect of Ginkgolide B on ferroptosis in high fat diet induced nonalcoholic fatty liver disease

Author:: Yang, Yuwei; Chen, Jing; Gao, Qin; Shan, Xin; Wang, Jie; Lv, Zhiyang
Source:: Toxicology 2020 v.445 pp. 152599
ISSN:: 0300-483X
Subject:: Ginkgo biloba; RNA interference; Western blotting; animal disease models; biomarkers; fatty liver; ferritin; ferroptosis; heme oxygenase (biliverdin-producing); high fat diet; histopathology; human cell lines; humans; immunohistochemistry; iron; iron overload; lipid metabolism; lipid peroxidation; mice; obesity; oleic acid; oxidative stress; palmitic acid; phospholipid-hydroperoxide glutathione peroxidase; plant extracts; signal transduction; small interfering RNA; transferrin
Abstract:: ... Ginkgolide B (GB), a main constituent of Ginkgo biloba extracts, reduces hepatic lipid accumulation and ameliorates nonalcoholic fatty liver disease (NAFLD) in obese mice, but the potential mechanism is unclear. Here we investigated the attenuated effects of GB on the disorder of lipid metabolism, oxidative stress and iron deposition in NAFLD and its potential mechanism associated with ferroptosis ...
DOI:: 10.1016/j.tox.2020.152599
: https://dx.doi.org/10.1016/j.tox.2020.152599

61. Activation of the Nrf2/HO-1 pathway by curcumin inhibits oxidative stress in human nasal fibroblasts exposed to urban particulate matter

Author:: Kim, Ji-Sun; Oh, Jeong-Min; Choi, Hyunsu; Kim, Sung Won; Kim, Soo Whan; Kim, Byung Guk; Cho, Jin Hee; Lee, Joohyung; Lee, Dong Chang
Source:: BMC complementary medicine and therapies 2020 v.20 no.1 pp. 101
ISSN:: 2662-7671
Subject:: Western blotting; antioxidant activity; chronic diseases; curcumin; dose response; fibroblasts; flow cytometry; humans; mitogen-activated protein kinase; nose; oxidative stress; particulates; patients; reactive oxygen species; rhinitis
Abstract:: ... BACKGROUND: Particulate matter (PM) can cause various negative acute and chronic diseases of the respiratory system, including the upper airways. Curcumin has been reported to have anti-inflammatory and anti-oxidative effects; therefore, we investigated the effects of curcumin on nasal fibroblasts exposed to urban PM (UPM). METHODS: Samples of inferior turbinate tissue were obtained from six patie ...
DOI:: 10.1186/s12906-020-02886-8
PubMed:: 32228565
PubMed Central:: PMC7106591
: https://dx.doi.org/10.1186/s12906-020-02886-8

62. Alisol A-24-acetate promotes glucose uptake via activation of AMPK in C2C12 myotubes

Author:: Chen, Jia-xiang; Li, Hai-yan; Li, Tian-tian; Fu, Wen-cheng; Du, Xin; Liu, Chun-hui; Zhang, Wen
Source:: BMC complementary medicine and therapies 2020 v.20 no.1 pp. 22
ISSN:: 2662-7671
Subject:: AMP-activated protein kinase; Alisma orientale; Alisols; Western blotting; calcium; calcium-calmodulin-dependent protein kinase; glucose; glucose transporters; glycemic effect; insulin; insulin receptor substrate proteins; mechanism of action; medicinal plants; metabolism; mitogen-activated protein kinase; myotubes; non-specific serine/threonine protein kinase; phosphorylation; plasma membrane; signal transduction; triterpenoids
Abstract:: ... BACKGROUND: Alisol A-24-acetate (AA-24-a) is one of the main active triterpenes isolated from the well-known medicinal plant Alisma orientale (Sam.) Juz., which possesses multiple biological activities, including a hypoglycemic effect. Whether AA-24-a is a hypoglycemic-active compound of A. orientale (Sam.) Juz. is unclear. The present study aimed to clarify the effect and potential mechanism of a ...
DOI:: 10.1186/s12906-019-2802-3
PubMed:: 32020870
PubMed Central:: PMC7076831
: https://dx.doi.org/10.1186/s12906-019-2802-3

63. Ampicillin triggers the release of Pal in toxic vesicles from Escherichia coli

Author:: Michel, Lea Vacca; Gallardo, Leslie; Konovalova, Anna; Bauer, Morgan; Jackson, Niaya; Zavorin, Mark; McNamara, Carlie; Pierce, Jeanetta; Cheng, Susan; Snyder, Emma; Hellman, Judith; Pichichero, Michael E.
Source:: International journal of antimicrobial agents 2020 v.56 no.6 pp. 106163
ISSN:: 0924-8579
Subject:: Escherichia coli; Western blotting; ampicillin; gentamicin; inflammation; levofloxacin; lipopolysaccharides; lipoproteins; nanoparticles; outer membrane proteins; toxicity; transmission electron microscopy; ultracentrifugation
Abstract:: ... In addition to lipopolysaccharides (LPS), outer membrane proteins – Lpp, OmpA and peptidoglycan-associated lipoprotein (Pal) – are part of the outer membrane of Escherichia coli and are proposed to contribute to bacterial sepsis-related inflammation. This study showed that ampicillin (a β-lactam antibiotic) enhances Pal's release from Escherichia coli to a greater extent than gentamicin and levofl ...
DOI:: 10.1016/j.ijantimicag.2020.106163
: https://dx.doi.org/10.1016/j.ijantimicag.2020.106163

64. Angiostrongylus cantonensis Galectin-1 interacts with Annexin A2 to impair the viability of macrophages via activating JNK pathway

Author:: Shi, Xiaomeng; Xiao, Mengran; Xie, Zhiyue; Shi, Qing; Zhang, Yuanjiao; Leavenworth, Jianmei W.; Yan, Baolong; Huang, Huicong
Source:: Parasites & vectors 2020 v.13 no.1 pp. 183
ISSN:: 1756-3305
Subject:: Parastrongylus cantonensis; Western blotting; affinity chromatography; apoptosis; blood; caspase-3; caspase-9; cell viability; central nervous system; cerebrospinal fluid; enzyme inhibition; flow cytometry; fluorescence microscopy; galectins; immune response; macrophages; mass spectrometry; membrane proteins; mice; microarray technology; mitogen-activated protein kinase; nickel; parasites; phosphorylation; precipitin tests; signal transduction
Abstract:: ... BACKGROUND: Angiostrongylus cantonensis can cause severe symptoms of central nervous system infections. In the host, this parasite localizes in the blood and cerebrospinal fluid, and its secreted components can impact immune responses. Our previous study demonstrated that immune responses were inhibited in A. cantonensis-infected mice immunized with Ac-Galectin-1 (AcGal-1). However, the mechanisms ...
DOI:: 10.1186/s13071-020-04038-w
PubMed:: 32268913
PubMed Central:: PMC7140382
: https://dx.doi.org/10.1186/s13071-020-04038-w

65. A novel protein chip for simultaneous detection of antibodies against four epidemic swine viruses in China

Author:: Wu, Yue; Wu, Xudan; Chen, Jing; Hu, Jingfei; Huang, Xiaobo; Zhou, Bin
Source:: BMC veterinary research 2020 v.16 no.1 pp. 162
ISSN:: 1746-6148
Subject:: Betaarterivirus suid 1; Bluetongue virus; Classical swine fever virus; Infectious bronchitis virus; Japanese encephalitis virus; Ungulate protoparvovirus 1; Western blotting; antibodies; blood serum; cross reaction; detection; enzyme-linked immunosorbent assay; glass; mixed infection; pathogens; plasmids; protein microarrays; proteins; research; ruminants; sampling; swine; swine diseases; veterinary medicine; viruses; China
Abstract:: ... BACKGROUND: At present, pig industry in China is faced with the complex situation of mixed infection caused by multiple pathogens. It is urgent to develop some new high-throughput molecular diagnosis assays to simultaneously detect pathogens or antibodies. Biochip array technology has made it possible to screen thousands of samples simultaneously; it has been twice named as one of the top 10 scien ...
DOI:: 10.1186/s12917-020-02375-7
: https://dx.doi.org/10.1186/s12917-020-02375-7

66. Astragalin alleviates cerebral ischemia-reperfusion injury by improving anti-oxidant and anti-inflammatory activities and inhibiting apoptosis pathway in rats

Author:: Chen, Xiuying; Cheng, Chang; Zuo, Xuzheng; Huang, Wen
Source:: BMC complementary medicine and therapies 2020 v.20 no.1 pp. 120
ISSN:: 2662-7671
Subject:: Western blotting; animal models; anti-inflammatory activity; antioxidant activity; antioxidant enzymes; apoptosis; brain; caspase-3; chlorides; flavonoids; glutathione; glutathione peroxidase; heme oxygenase (biliverdin-producing); herbs; infarction; malondialdehyde; medicinal plants; neurons; nitric oxide; prostaglandin synthase; protein synthesis; rats; signal transduction; staining; superoxide dismutase; transcription factor NF-kappa B
Abstract:: ... BACKGROUND: Astragalin (AG), a flavonoid from many traditional herbs and medicinal plants, has been described to exhibit in vitro anti-inflammatory activity. The paper aimed to study the effects of astragalin on anti-inflammatory, anti-oxidative ability and apoptosis signaling pathway in brain tissue of rats with cerebral ischemia-reperfusion injury, and to explore its possible mechanism. METHODS: ...
DOI:: 10.1186/s12906-020-02902-x
PubMed:: 32316944
PubMed Central:: PMC7171805
: https://dx.doi.org/10.1186/s12906-020-02902-x

67. Betaine attenuates LPS-induced downregulation of Occludin and Claudin-1 and restores intestinal barrier function

Author:: Wu, Jingtao; He, Caimei; Bu, Jie; Luo, Yue; Yang, Shuyuan; Ye, Chengyan; Yu, Silei; He, Binsheng; Yin, Yulong; Yang, Xiaoping
Source:: BMC veterinary research 2020 v.16 no.1 pp. 75
ISSN:: 1746-6148
Subject:: Western blotting; anti-inflammatory activity; betaine; electrical resistance; epithelial cells; epithelium; gastrointestinal diseases; inflammation; intestines; lipopolysaccharides; models; occludins; protective effect; reverse transcriptase polymerase chain reaction; swine; tight junctions
Abstract:: ... BACKGROUND: The intestinal epithelial barrier, which works as the first line of defense between the luminal environment and the host, once destroyed, it will cause serious inflammation or other intestinal diseases. Tight junctions (TJs) play a vital role to maintain the integrity of the epithelial barrier. Lipopolysaccharide (LPS), one of the most important inflammatory factors will downregulate s ...
DOI:: 10.1186/s12917-020-02298-3
PubMed:: 32131830
PubMed Central:: PMC7057534
: https://dx.doi.org/10.1186/s12917-020-02298-3

68. Betaine attenuates LPS-induced downregulation of Occludin and Claudin-1 and restores intestinal barrier function

Author:: Wu, Jingtao; He, Caimei; Bu, Jie; Luo, Yue; Yang, Shuyuan; Ye, Chengyan; Yu, Silei; He, Binsheng; Yin, Yulong; Yang, Xiaoping
Source:: BMC veterinary research 2020 v.16 no.1 pp. 75
ISSN:: 1746-6148
Subject:: Western blotting; anti-inflammatory activity; betaine; electrical resistance; environment; epithelial cells; epithelium; gastrointestinal diseases; inflammation; intestines; lipopolysaccharides; models; occludins; protective effect; research; reverse transcriptase polymerase chain reaction; swine; tight junctions; veterinary medicine
Abstract:: ... BACKGROUND: The intestinal epithelial barrier, which works as the first line of defense between the luminal environment and the host, once destroyed, it will cause serious inflammation or other intestinal diseases. Tight junctions (TJs) play a vital role to maintain the integrity of the epithelial barrier. Lipopolysaccharide (LPS), one of the most important inflammatory factors will downregulate s ...
DOI:: 10.1186/s12917-020-02298-3
: https://dx.doi.org/10.1186/s12917-020-02298-3

69. Bovine adapted transmissible mink encephalopathy is similar to L-BSE after passage through sheep with the VRQ/VRQ genotype but not VRQ/ARQ

Author:: Cassmann, Eric; Moore, Sarah Jo; Kokemuller, Robyn; Balkema-Buschmann, Anne; Groschup, Martin; Nicholson, Eric; Greenlee, Justin
Source:: BMC veterinary research 2020 v.16 no.1 pp. 383
ISSN:: 1746-6148
Subject:: Western blotting; cattle; disease transmission; genetically modified organisms; genotype; humans; inoculum; mice; mink; monitoring; neuropathology; phenotype; prions; sheep; strains; transmissible mink encephalopathy
Abstract:: ... BACKGROUND: Transmissible mink encephalopathy (TME) is a fatal neurologic disease of farmed mink. Evidence indicates that TME and L-BSE are similar and may be linked in some outbreaks of TME. We previously transmitted bovine adapted TME (bTME) to sheep. The present study compared ovine passaged bTME (o-bTME) to C-BSE and L-BSE in transgenic mice expressing wild type bovine prion protein (TgBovXV). ...
DOI:: 10.1186/s12917-020-02611-0
: https://dx.doi.org/10.1186/s12917-020-02611-0

70. Buyang Huanwu Tang inhibits cellular epithelial-to-mesenchymal transition by inhibiting TGF-β1 activation of PI3K/Akt signaling pathway in pulmonary fibrosis model in vitro

Author:: Yin, Zi-fei; Wei, Yang-lin; Wang, Xuan; Wang, Li-na; Li, Xia
Source:: BMC complementary medicine and therapies 2020 v.20 no.1 pp. 13
ISSN:: 2662-7671
Subject:: Oriental traditional medicine; Western blotting; cadherins; cell growth; cell structures; cell viability; collagen; enzyme inhibition; epithelium; fibroblasts; fibrosis; fluorescent antibody technique; humans; insulin-like growth factor I; messenger RNA; models; phosphatidylinositol 3-kinase; phosphorylation; quantitative polymerase chain reaction; respiratory tract diseases; signal transduction; staining; toxicity testing; transforming growth factor beta 1; vimentin; China
Abstract:: ... BACKGROUND: Pulmonary fibrosis (PF) is a chronic and progressive interstitial lung disease. Buyang Huanwu Tang (BYHWT), a classical traditional Chinese medicine formula, has been widely utilized for the treatment of PF in China. This present study aimed to explore the mechanism of BYHWT in the treatment of PF in vitro. METHODS: TGF-β1 stimulated human alveolar epithelial A549 cells were used as in ...
DOI:: 10.1186/s12906-019-2807-y
PubMed:: 32020862
PubMed Central:: PMC7076841
: https://dx.doi.org/10.1186/s12906-019-2807-y

71. Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity

Author:: Villatoro, Antonio J.; Martín-Astorga, María del Carmen; Alcoholado, Cristina; Becerra, José
Source:: BMC veterinary research 2020 v.16 no.1 pp. 417
ISSN:: 1746-6148
Subject:: Western blotting; adipose tissue; angiogenesis; antioxidant activity; bone marrow; chemotaxis; colostrum; dogs; exosomes; fibroblasts; hematopoiesis; immunity; immunoglobulins; mammary glands; mesenchymal stromal cells; milk; neonates; neurogenesis; proteomics; reactive oxygen species; secretion; veterinary medicine
Abstract:: ... BACKGROUND: Canine colostrum milk (CCM) is a specific secretion of the mammary gland that is fundamental for the survival of the newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown. RESULTS: A characterization of CCM exosomes was performed. Exosomes were abundant in CCM and appeared with the characteristic cup-s ...
DOI:: 10.1186/s12917-020-02623-w
: https://dx.doi.org/10.1186/s12917-020-02623-w

72. Cannabidiol induces osteoblast differentiation via angiopoietin1 and p38 MAPK

Author:: Kang, Mi‐Ae; Lee, Jongsung; Park, See‐Hyoung
Source:: Environmental toxicology 2020 v.35 no.12 pp. 1318-1325
ISSN:: 1520-4081
Subject:: Cannabis; Western blotting; alizarin; alkaline phosphatase; assays; calcium; cannabidiol; collagen; ecotoxicology; mineralization; mitogen-activated protein kinase; osteoblasts; osteocalcin; protein-protein interactions; staining; therapeutics; tight junctions; transcription factors
Abstract:: ... In this study, we report the potential of cannabidiol, one of the major cannabis constituents, for enhancing osteoblastic differentiation in U2OS and MG‐63 cells. Cannabidiol increased the expression of Angiopoietin1 and the enzyme activity of alkaline phosphatase in U2OS and MG‐63. Invasion and migration assay results indicated that the cell mobility was activated by cannabidiol in U2OS and MG‐63 ...
DOI:: 10.1002/tox.22996
: http://dx.doi.org/10.1002/tox.22996

73. Cepharanthine and Curcumin inhibited mitochondrial apoptosis induced by PCV2

Author:: Xu, Yinlan; Zheng, Jiangang; Sun, Panpan; Guo, Jianhua; Zheng, Xiaozhong; Sun, Yaogui; Fan, Kuohai; Yin, Wei; Li, Hongquan; Sun, Na
Source:: BMC veterinary research 2020 v.16 no.1 pp. 345
ISSN:: 1746-6148
Subject:: Porcine circovirus-2; Western blotting; antiviral properties; apoptosis; assays; caspase-3; cells; chemical structure; curcumin; cytotoxicity; dose response; farms; financial economics; genotype; immunosuppression; mitochondria; mixed infection; mutation; pathogens; prevalence; swine; vaccination; veterinary medicine
Abstract:: ... BACKGROUND: Porcine circovirus type 2 (PCV2) is an immunosuppressive pathogen with high prevalence rate in pig farms. It has caused serious economic losses to the global pig industry. Due to the rapid mutation of PCV2 strain and co-infection of different genotypes, vaccination could not eradicate the infection of PCV2. It is necessary to screen and develop effective new compounds and explore their ...
DOI:: 10.1186/s12917-020-02568-0
: https://dx.doi.org/10.1186/s12917-020-02568-0

74. Chaenomelis fructus inhibits osteoclast differentiation by suppressing NFATc1 expression and prevents ovariectomy-induced osteoporosis

Author:: Kim, Minsun; Kim, Ho-Seok; Kim, Jae-Hyun; Kim, Eun-Young; Lee, Bina; Lee, Sung Yub; Jun, Jae-Yun; Kim, Min Beom; Sohn, Youngjoo; Jung, Hyuk-Sang
Source:: BMC complementary medicine and therapies 2020 v.20 no.1 pp. 35
ISSN:: 2662-7671
Subject:: T-lymphocytes; Western blotting; acid phosphatase; adenosinetriphosphatase; animal experimentation; bone density; bone resorption; carbonate dehydratase; cathepsin K; eosin; immunohistochemistry; in vitro studies; loss prevention; models; osteoclasts; osteoporosis; ovariectomy; protons; rats; reverse transcriptase polymerase chain reaction; signal transduction; staining; transcription factors
Abstract:: ... BACKGROUND: Osteoporosis is related to the number and activity of osteoclasts. The goal of the present study was to demonstrate the effect of Chaenomelis Fructus (CF) on osteoclastogenesis and its mechanism of bone loss prevention in an OVX-induced osteoporosis model. METHODS: Osteoclasts were induced by RANKL in RAW 264.7 cells. TRAP assay was performed to measure the inhibitory effect of CF on o ...
DOI:: 10.1186/s12906-020-2841-9
PubMed:: 32024503
PubMed Central:: PMC7076887
: https://dx.doi.org/10.1186/s12906-020-2841-9

75. Characterization of a recombinant pseudorabies virus expressing porcine parvovirus VP2 protein and porcine IL-6

Author:: Zheng, Hui-Hua; Wang, Lin-Qing; Fu, Peng-Fei; Zheng, Lan-Lan; Chen, Hong-Ying; Liu, Fang
Source:: Virology journal 2020 v.17 no.1 pp. 19
ISSN:: 1743-422X
Subject:: Suid alphaherpesvirus 1; Western blotting; animal viruses; financial economics; genes; growth curves; homologous recombination; immune response; immunogenicity; infectious diseases; interleukin-6; intramuscular injection; live vaccines; lymphocyte proliferation; mice; morphogenesis; mortality; neutralizing antibodies; plasmids; pork industry; reverse transcriptase polymerase chain reaction; sequence analysis; swine; swine diseases; testes; viral load; virulence; viruses
Abstract:: ... BACKGROUND: Porcine parvovirus (PPV) and pseudorabies virus (PRV) are the important etiological agents of swine infectious diseases, resulting in huge economic losses to the Chinese swine industry. Interleukin-6 (IL-6) has the roles to support host immune response to infections as a pleiotropic cytokine. It is essential to construct a live attenuated vaccine-based recombinant PRV that expresses PP ...
DOI:: 10.1186/s12985-020-1292-8
PubMed:: 32014014
PubMed Central:: PMC6998180
: https://dx.doi.org/10.1186/s12985-020-1292-8

76. Characterization of the hypoxia-inducible factor-1 pathway in hearts of Antarctic notothenioid fishes

Author:: O'Brien, K.M.; Rix, A.S.; Grove, T.J.; Sarrimanolis, J.; Brooking, A.; Roberts, M.; Crockett, E.L.
Source:: Comparative biochemistry and physiology 2020 v.250 pp. 110505
ISSN:: 1096-4959
Subject:: Chaenocephalus aceratus; Gobionotothen gibberifrons; Notothenia coriiceps; Western blotting; anaerobiosis; genes; global warming; heart; heat stress; heat tolerance; homeostasis; hypoxia; hypoxia-inducible factor 1; lactic acid; messenger RNA; oxygen; protein content
Abstract:: ... The ability of Antarctic notothenioid fishes to mount a robust molecular response to hypoxia is largely unknown. The transcription factor, hypoxia-inducible factor-1 (HIF-1), a heterodimer of HIF-1α and HIF-1β subunits, is the master regulator of oxygen homeostasis in most metazoans. We sought to determine if, in the hearts of Antarctic notothenioids, HIF-1 is activated and functional in response ...
DOI:: 10.1016/j.cbpb.2020.110505
: http://dx.doi.org/10.1016/j.cbpb.2020.110505

77. Chinese medicine Di-Huang-Yi-Zhi protects PC12 cells from H2O2-induced apoptosis by regulating ROS-ASK1-JNK/p38 MAPK signaling

Author:: Zhang, Li-Min; Zhen, Rong-Rong; Gu, Chao; Zhang, Tian-Li; Li, Yue; Jin, Miao; Hu, Bing; An, Hong-Mei
Source:: BMC complementary medicine and therapies 2020 v.20 no.1 pp. 54
ISSN:: 2662-7671
Subject:: Alzheimer disease; Oriental traditional medicine; Western blotting; apoptosis; caspase-3; cell proliferation; cytotoxicity; enzyme activity; enzyme inhibition; hydrogen peroxide; lactate dehydrogenase; membrane potential; mitochondrial membrane; mitogen-activated protein kinase; nerve tissue; oxidative stress; pathogenesis; phosphorylation; protein synthesis; staining
Abstract:: ... BACKGROUND: Oxidative stress mediates the nerve injury during the pathogenesis of Alzheimer’s disease (AD). Protecting against oxidative stress damage is an important strategy to prevent and treat AD. Di-Huang-Yi-Zhi (DHYZ) is a Chinese medicine used for the treatment of AD, but its mechanism remains unknown. This study is aimed to investigate the effect of DHYZ on H₂O₂ induced oxidative damage in ...
DOI:: 10.1186/s12906-020-2834-8
PubMed:: 32059723
PubMed Central:: PMC7076825
: https://dx.doi.org/10.1186/s12906-020-2834-8

78. Circaea mollis Siebold & Zucc. Alleviates postmenopausal osteoporosis in a mouse model via the BMP-2/4/Runx2 pathway

Author:: Park, Ji Hye; Son, Yang Ju; Lee, Chang Ho; Nho, Chu Won; Yoo, Gyhye
Source:: BMC complementary medicine and therapies 2020 v.20 no.1 pp. 123
ISSN:: 2662-7671
Subject:: Western blotting; alkaline phosphatase; animal disease models; bone density; bone formation; bone resorption; breast neoplasms; coculture; enzyme activity; ethanol; females; herbal medicines; in vitro studies; in vivo studies; mice; neoplasm cells; osteoblasts; osteoclasts; osteoporosis; ovariectomy; postmenopause; quantitative polymerase chain reaction
Abstract:: ... BACKGROUND: Circaea mollis Sieb. & Zucc. has been used as a traditional herbal medicine in Hani Ethnopharmacy and possesses anti-arthritic activities. This study aimed to investigate the effect of Circaea mollis Siebold & Zucc on postmenopausal osteoporosis. METHODS: For in vitro study, MCF7 breast cancer cells and MC3T3-E1 pre-osteoblast cells were utilized to estimate estrogenic and osteogenic a ...
DOI:: 10.1186/s12906-020-02914-7
PubMed:: 32321506
PubMed Central:: PMC7178630
: https://dx.doi.org/10.1186/s12906-020-02914-7

79. Circular RNA hsa_circ_0004812 impairs IFN-induced immune response by sponging miR-1287-5p to regulate FSTL1 in chronic hepatitis B

Author:: Zhang, Liangdong; Wang, Zichao
Source:: Virology journal 2020 v.17 no.1 pp. 40
ISSN:: 1743-422X
Subject:: Western blotting; biotin; blood; chronic hepatitis B; circular RNA; hepatoma; immune response; in vitro studies; interferon-alpha; interferon-beta; liver; luciferase; patients; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; tissues; volunteers
Abstract:: ... BACKGROUND: The present study aims to explore the functions of circular RNA hsa_circ_0004812 in chronic hepatitis B (CHB) and its underlying molecular mechanisms. METHODS: The expression of circular RNA (circRNA)_0004812 was examined using qRT-PCR and Western blot in blood and liver tissues from CHB patients and healthy volunteers. In the in vitro study, the expression levels of circular RNA hsa_c ...
DOI:: 10.1186/s12985-020-01314-0
PubMed:: 32188476
PubMed Central:: PMC7079541
: https://dx.doi.org/10.1186/s12985-020-01314-0

80. Cloning of Hynobius lichenatus (Tohoku hynobiid salamander) p53 and analysis of its expression in response to radiation

Author:: Kamada, Toshiki; Une, Yumi; Matsui, Kumi; Fuma, Shoichi; Ikeda, Teruo; Okamoto, Mariko
Source:: BMC genetics 2020 v.21 no.1 pp. 53
ISSN:: 1471-2156
Subject:: DNA damage; Hynobius; Western blotting; X-radiation; actin; complementary DNA; gamma radiation; gene expression; gene expression regulation; genes; glyceraldehyde-3-phosphate dehydrogenase; human cell lines; irradiation; mammals; messenger RNA; nucleotide sequences; quantitative polymerase chain reaction; rapid amplification of cDNA ends; reverse transcriptase polymerase chain reaction; salamanders and newts; transfection
Abstract:: ... BACKGROUND: Caudata species such as salamanders are easily affected by environmental changes, which can drastically reduce their population. The effects of acute X-rays and chronic γ-irradiation on Hynobius lichenatus, the Japanese Tohoku hynobiid salamander, are known. However, the expression of radiation-inducible genes, such as the DNA-damage checkpoint response gene p53, has not been analyzed ...
DOI:: 10.1186/s12863-020-00856-0
: https://dx.doi.org/10.1186/s12863-020-00856-0

81. Cranberry extract initiates intrinsic apoptosis in HL-60 cells by increasing BAD activity through inhibition of AKT phosphorylation

Author:: Mansouri, Rasha A.; Percival, Susan S.
Source:: BMC complementary medicine and therapies 2020 v.20 no.1 pp. 71
ISSN:: 2662-7671
Subject:: B-lymphocytes; Western blotting; antibodies; antineoplastic activity; antineoplastic agents; apoptosis; caspase-9; cranberries; cytochrome c; death; fruit extracts; in vivo studies; membrane permeability; mitochondria; neoplasm cells; neoplasms; non-specific serine/threonine protein kinase; phosphorylation
Abstract:: ... BACKGROUND: Cranberry has been studied as a potential anticancer agent as it is capable of inducing apoptosis within cancer cells. The aim of this study was to better define the mechanism by which cranberry triggers apoptosis in HL-60 cells. METHODS: The study was carried on cranberry extracts (CB). Anti-apoptotic B-cell lymphoma-2 (BCL-2) and pro-apoptotic BCL-2-associated death promoter death (B ...
DOI:: 10.1186/s12906-020-2870-4
PubMed:: 32143616
PubMed Central:: PMC7076838
: https://dx.doi.org/10.1186/s12906-020-2870-4

82. Crucial role of androgen receptor in resistance and endurance trainings-induced muscle hypertrophy through IGF-1/IGF-1R- PI3K/Akt- mTOR pathway

Author:: YIN, Lijun; LU, Lin; LIN, Xiaojing; WANG, Xiaohui
Source:: Nutrition & metabolism 2020 v.17 no.1 pp. 26
ISSN:: 1743-7075
Subject:: Western blotting; androgen receptors; antagonists; blood serum; body weight; enzyme activity; enzyme-linked immunosorbent assay; genes; hypertrophy; insulin; insulin-like growth factor I; insulin-like growth factor I receptor; laboratory animals; males; messenger RNA; muscle tissues; muscles; phosphatidylinositol 3-kinase; protein content; quantitative polymerase chain reaction; rapamycin; rats; strength training; testosterone
Abstract:: ... BACKGROUND: Androgen receptor (AR) has been reported to play vital roles in exercise-induced increase of muscle mass in rats, but needs to be further verified and the mechanism behind remains unclear. As AR target genes, insulin growth factor-1 (IGF-1) and IGF-1 receptor (IGF-1R) promote muscle hypertrophy through activating PI3K/Akt- mammalian target of rapamycin (mTOR) pathway, a classic pathway ...
DOI:: 10.1186/s12986-020-00446-y
PubMed:: 32256674
PubMed Central:: PMC7106900
: https://dx.doi.org/10.1186/s12986-020-00446-y

83. Cudrania tricuspidata leaf extracts and its components, chlorogenic acid, kaempferol, and quercetin, increase claudin 1 expression in human keratinocytes, enhancing intercellular tight junction capacity

Author:: Kim, Jaewhan; Cho, Namjoon; Kim, Eun-Mi; Park, Ki-Sun; Kang, Yeon Woo; Nam, Joong Hyeon; Nam, Myoung Soo; Kim, Kee K.
Source:: Applied biological chemistry 2020 v.63 no.1 pp. 23
ISSN:: 2468-0834
Subject:: Maclura tricuspidata; Western blotting; atopic dermatitis; cell proliferation; cell viability; chlorogenic acid; dose response; enzymes; ethanol; fluorescence microscopy; gene expression regulation; humans; kaempferol; keratinocytes; leaf extracts; messenger RNA; protein synthesis; psoriasis; quantitative polymerase chain reaction; quercetin; reverse transcriptase polymerase chain reaction; therapeutics; tight junctions
Abstract:: ... Dysfunction of tight junctions and their components can cause diverse skin diseases. Here, we investigated the expression of claudin 1, a major tight junction protein, and changes of tight junction capacity upon treatment of the extracts of Cudrania tricuspidata (C. tricuspidata) and its components, chlorogenic acid, kaempferol, and quercetin. The effects of ethanol extracts of C. tricuspidata (EE ...
DOI:: 10.1186/s13765-020-00505-1
: https://dx.doi.org/10.1186/s13765-020-00505-1

84. Curcumin downregulates the PI3K–AKT–mTOR pathway and inhibits growth and progression in head and neck cancer cells

Author:: Borges, Gabriel Alvares; Elias, Silvia Taveira; Amorim, Bruna; de Lima, Caroline Lourenço; Coletta, Ricardo Della; Castilho, Rogerio Moraes; Squarize, Cristiane Helena; Guerra, Eliete Neves Silva
Source:: Phytotherapy research 2020 v.34 no.12 pp. 3311-3324
ISSN:: 0951-418X
Subject:: Curcuma longa; Western blotting; anti-inflammatory activity; antioxidants; apoptosis; carcinoma; cell cycle; cell lines; cell viability; curcumin; cytoskeleton; dose response; flow cytometry; genes; head and neck neoplasms; keratinocytes; necrosis; neoplasm cells; phytotherapy; polyphenols; protein synthesis; proteins; research; rhizomes; signal transduction; staining; tongue
Abstract:: ... Curcumin, a polyphenol isolated from the rhizome of Curcuma longa, has been studied because of its antioxidant, antimicrobial, and antiinflammatory properties. This study aimed to evaluate the effects of curcumin on head and neck cancer (HNC) cell lines and how it modulates the PI3K–AKT–mTOR signaling pathway. Dose‐response curves for curcumin were established for hypopharynx carcinoma (FaDu), ton ...
DOI:: 10.1002/ptr.6780
: http://dx.doi.org/10.1002/ptr.6780

85. Cyanidin-3-glucoside activates Nrf2-antioxidant response element and protects against glutamate-induced oxidative and endoplasmic reticulum stress in HT22 hippocampal neuronal cells

Author:: Sukprasansap, Monruedee; Chanvorachote, Pithi; Tencomnao, Tewin
Source:: BMC complementary medicine and therapies 2020 v.20 no.1 pp. 46
ISSN:: 2662-7671
Subject:: Western blotting; anthocyanins; antioxidant activity; antioxidant enzymes; antioxidant genes; apoptosis; calpain; caspase-12; catalase; cell viability; endoplasmic reticulum stress; flow cytometry; gene expression; glutamic acid; glutathione peroxidase; glutathione transferase; mice; mitogen-activated protein kinase; necroptosis; neurodegenerative diseases; neurons; neuroprotective effect; oxidative toxicity; propidium; proteins; quantitative polymerase chain reaction; reactive oxygen species; signal transduction; small fruits; superoxide dismutase
Abstract:: ... BACKGROUND: Cyanidin-3-glucoside (C3G), a major anthocyanin present in berries, exhibits a strong antioxidant and has been shown to possess a neuroprotection. Prolonged exposure to glutamate will lead to oxidative damage and endoplasmic reticulum stress which could play a key detrimental role in the development of neurodegenerative disorders (NDs). In the present study, we investigated the neuropr ...
DOI:: 10.1186/s12906-020-2819-7
PubMed:: 32046712
PubMed Central:: PMC7076852
: https://dx.doi.org/10.1186/s12906-020-2819-7

86. Dasatinib reverses drug resistance by downregulating MDR1 and Survivin in Burkitt lymphoma cells

Author:: Tabata, Mitsuki; Tsubaki, Masanobu; Takeda, Tomoya; Tateishi, Keisuke; Tsurushima, Katsumasa; Imano, Motohiro; Satou, Takao; Ishizaka, Toshihiko; Nishida, Shozo
Source:: BMC complementary medicine and therapies 2020 v.20 no.1 pp. 84
ISSN:: 2662-7671
Subject:: Western blotting; cell lines; cell viability; cross resistance; dexamethasone; doxorubicin; drug resistance; drug therapy; dyes; enzyme activity; enzymes; gene expression; inhibitory concentration 50; lymphoma; messenger RNA; neoplasm cells; patients; polymerase chain reaction; pro-apoptotic proteins; prognosis; small interfering RNA; verapamil; vincristine
Abstract:: ... BACKGROUND: Current chemotherapies for Burkitt lymphoma (BL) have dramatically improved its clinical outcome. However, chemoresistance can lead to chemotherapy failure and very poor prognosis; thus, novel strategies are urgently required for patients with drug-resistant BL. To investigate the mechanisms underlying drug resistance in BL, we established drug-resistant BL cell lines: HS-Sultan/ADM (a ...
DOI:: 10.1186/s12906-020-2879-8
PubMed:: 32171300
PubMed Central:: PMC7076888
: https://dx.doi.org/10.1186/s12906-020-2879-8

87. Decursinol from Angelica gigas Nakai enhances endometrial receptivity during implantation

Author:: Kim, Seong-Eun; Lee, Joo Eun; Han, Young-Hyun; Lee, Se-In; Kim, Do Kyung; Park, Seok-Rae; Yu, Seong-Lan; Kang, Jaeku
Source:: BMC complementary medicine and therapies 2020 v.20 no.1 pp. 36
ISSN:: 2662-7671
Subject:: Angelica gigas; CD29 antigen; Western blotting; adhesion; autocrine signaling; cell adhesion molecules; dimethyl sulfoxide; embryo implantation; endometrium; epithelial cells; exosomes; herbal medicines; herbs; messenger RNA; models; patients; pregnancy; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; trophoblast
Abstract:: ... BACKGROUND: Embryo implantation is essential for a successful pregnancy, and an elaborate synchronization between the receptive endometrium and trophoblast is required to achieve this implantation. To increase ‘endometrial receptivity’, the endometrium undergoes transformation processes including responses of adhesion molecules and cellular and molecular cell to cell communication. Many natural su ...
DOI:: 10.1186/s12906-020-2822-z
PubMed:: 32024510
PubMed Central:: PMC7076876
: https://dx.doi.org/10.1186/s12906-020-2822-z

88. Determination of c-Abl tyrosine kinase and mTERT catalytic subunit of telomerase expression level during prenatal-postnatal mouse ovary-testis development

Author:: Yildirim, Ecem; Yaba, Aylin
Source:: Reproductive Biology 2020 v.20 no.4 pp. 555-567
ISSN:: 1642-431X
Subject:: Western blotting; adults; animal ovaries; cell proliferation; enzyme activity; females; fluorescent antibody technique; genes; germ cells; hormones; males; mice; non-specific protein-tyrosine kinase; postpartum period; protein subunits; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; staining; telomerase; testes
Abstract:: ... Expression levels of genes involved in the development of germ cells vary throughout the process from bipotential gonadal period to adult gonadal formation. In mice, developments of female and male reproductive system are regulated by germ cell-specific factors and hormones, and determinative days in this regulation are very important. c-Abl is a non-receptor tyrosine kinase with cellular function ...
DOI:: 10.1016/j.repbio.2020.10.004
: https://dx.doi.org/10.1016/j.repbio.2020.10.004

89. Dietary alterations modulate the microRNA 29/30 and IGF-1/AKT signaling axis in breast Cancer liver metastasis

Author:: Shastri, Anuradha A.; Saleh, Anthony; Savage, Jason E.; DeAngelis, Tiziana; Camphausen, Kevin; Simone, Nicole L.
Source:: Nutrition & metabolism 2020 v.17 no.1 pp. 23
ISSN:: 1743-7075
Subject:: Western blotting; breast neoplasms; colorectal neoplasms; insulin-like growth factor I; insulin-like growth factor I receptor; liver; low calorie diet; metastasis; mice; microRNA; models; patients; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; signal transduction
Abstract:: ... BACKGROUND: Metastatic cancer is incurable and understanding the molecular underpinnings is crucial to improving survival for our patients. The IGF-1/Akt signaling pathway is often impaired in cancer leading to its progression and metastases. Diet modification is known to alter the IGF-1/Akt pathway and affect the expression of microRNA involved in tumor initiation, growth and metastases. Liver me ...
DOI:: 10.1186/s12986-020-00437-z
PubMed:: 32211051
PubMed Central:: PMC7092508
: https://dx.doi.org/10.1186/s12986-020-00437-z

90. Differential nitric oxide induced by Mycobacterium bovis and BCG leading to dendritic cells apoptosis in a caspase dependent manner

Author:: Liu, Han; Xiong, Xuekai; Zhu, Tingting; Zhu, Yifan; Peng, Yongchong; Zhu, Xiaojie; Wang, Jieru; Chen, Huanchun; Chen, Yingyu; Guo, Aizhen
Source:: Microbial pathogenesis 2020 v.149 pp. 104303
ISSN:: 0882-4010
Subject:: DNA fragmentation; Mycobacterium bovis BCG; Mycobacterium tuberculosis; Western blotting; adaptive immunity; apoptosis; arginine; caspase-3; caspase-8; caspase-9; cytochrome c; dendritic cells; enzyme inhibitors; flow cytometry; gene expression; gene expression regulation; genes; inducible nitric oxide synthase; innate immunity; interferon-gamma; metabolism; mice; mitochondria; nitric oxide; pathogenesis; quantitative polymerase chain reaction; reactive nitrogen species; reverse transcriptase polymerase chain reaction; urea
Abstract:: ... Dendritic cells (DCs) are critical for both innate and adaptive immunity. Meanwhile, nitric oxide (NO) is a member of reactive nitrogen species (RNS) generally considered to play a key role in the bactericidal process in innate immunity against Mycobacterium tuberculosis complex infection. The present study therefore investigated the mechanism of NO production in murine DCs induced by Mycobacteriu ...
DOI:: 10.1016/j.micpath.2020.104303
: https://dx.doi.org/10.1016/j.micpath.2020.104303

91. Distinct and overlapping effects of β2-glycoprotein I conformational variants in ligand interactions and functional assays

Author:: Szabó, Gábor; Pénzes, Krisztina; Torner, Bernadett; Fagyas, Miklós; Tarr, Tünde; Soltész, Pál; Kis, Gréta; Antal, Miklós; Kappelmayer, János
Source:: Journal of immunological methods 2020 v.487 pp. 112877
ISSN:: 0022-1759
Subject:: Western blotting; autoantibodies; coagulation; dissociation; electron microscopy; fibrin; fluorescence; heparin; humans; ligands; prothrombin; surface plasmon resonance; thrombin; thromboplastin
Abstract:: ... One of the most abundant coagulation proteins is β₂-glycoprotein I (β₂GPI) that is present in humans at a concentration of around 200 mg/L. Its physiological role is only partially understood, but it adopts several different structural forms the majority of which are the open and closed forms. We isolated native (circular) β₂GPI and converted it into an open conformation. The effectiveness of thes ...
DOI:: 10.1016/j.jim.2020.112877
: https://dx.doi.org/10.1016/j.jim.2020.112877

92. Disulfide isomerase ERp57 improves the stability and immunogenicity of H3N2 influenza virus hemagglutinin

Author:: Wu, Jialing; Wang, Yang; Wei, Ying; Xu, Zhichao; Tan, Xin; Wu, Zhihui; Zheng, Jing; Liu, George Dacai; Cao, Yongchang; Xue, Chunyi
Source:: Virology journal 2020 v.17 no.1 pp. 55
ISSN:: 1743-422X
Subject:: Influenza A virus; T-lymphocytes; Western blotting; disulfides; flow cytometry; fluorescent antibody technique; hemagglutinins; immunogenicity; immunoglobulin G; mice; protein disulfide-isomerase; quantitative polymerase chain reaction; small interfering RNA; survival rate; vaccines
Abstract:: ... BACKGROUND: Hemagglutinin (HA), as the surface immunogenic protein, is the most important component of influenza viruses. Previous studies showed that the stability of HA was significant for HA’s immunogenicity, and many efforts have been made to stabilize the expressed HA proteins. METHODS: In this study, the protein disulfide isomerases (PDIs) were investigated for the ability to improve the sta ...
DOI:: 10.1186/s12985-020-01325-x
PubMed:: 32316996
PubMed Central:: PMC7175539
: https://dx.doi.org/10.1186/s12985-020-01325-x

93. Early pregnancy affects the expression of toll-like receptor pathway in ovine thymus

Author:: Li, Ning; Wang, Luyu; Cao, Nan; Zhang, Leying; Han, Xu; Yang, Ling
Source:: Reproductive Biology 2020 v.20 no.4 pp. 547-554
ISSN:: 1642-431X
Subject:: Toll-like receptor 2; Toll-like receptor 3; Toll-like receptor 4; Toll-like receptor 5; Western blotting; adaptive immunity; epithelium; estrous cycle; ewes; genes; immunohistochemistry; interleukin-1; messenger RNA; non-specific serine/threonine protein kinase; pregnancy; quantitative polymerase chain reaction; thymus gland; tumor necrosis factor receptors
Abstract:: ... Toll-like receptors (TLRs) participates in regulation of the maternal immune tolerance during pregnancy, and the thymus is critical for the adaptive immune system. This study hypothesized whether early pregnancy affected the expression of toll-like receptor pathway in the thymus of ewes. In this study, expression of TLRs, tumor necrosis factor receptor associated factor 6 (TRAF6), interleukin 1 re ...
DOI:: 10.1016/j.repbio.2020.10.003
: https://dx.doi.org/10.1016/j.repbio.2020.10.003

94. Effect of apo-lactoferrin on leukotoxin and outer membrane vesicles of Mannheimia haemolytica A2

Author:: Avalos-Gómez, Christian; Reyes-López, Magda; Ramírez-Rico, Gerardo; Díaz-Aparicio, Efrén; Zenteno, Edgar; González-Ruiz, Cynthia; de la Garza, Mireya
Source:: Veterinary research 2020 v.51 no.1 pp. 36
ISSN:: 1297-9716
Subject:: B-lymphocytes; Gram-negative bacteria; Mannheimia haemolytica; Western blotting; adjuvants; antibacterial properties; farms; financial economics; flow cytometry; glycoproteins; goats; innate immunity; iron; lactoferrin; leukotoxins; lipopolysaccharides; minimum inhibitory concentration; permeability; polymyxin B; secretion; serotypes; sheep; silver staining; transmission electron microscopy; virulence; viruses
Abstract:: ... Mannheimia haemolytica serotype A2 is the principal cause of pneumonic mannheimiosis in ovine and caprine livestock; this disease is a consequence of immune suppression caused by stress and associated viruses and is responsible for significant economic losses in farm production worldwide. Gram-negative bacteria such as M. haemolytica produce outer membrane (OM)-derived spherical structures named o ...
DOI:: 10.1186/s13567-020-00759-z
PubMed:: 32138772
PubMed Central:: PMC7059318
: https://dx.doi.org/10.1186/s13567-020-00759-z

95. Effect of quercetin on chondrocyte phenotype and extracellular matrix expression

Author:: GUI, Zhi-Peng; HU, Yue; ZHOU, Yu-Ning; LIN, Kai-Li; XU, Yuan-Jin
Source:: Chinese journal of natural medicines 2020 v.18 no.12 pp. 922-933
ISSN:: 1875-5364
Subject:: Oriental traditional medicine; Western blotting; assays; cartilage; chondrogenesis; dose response; extracellular matrix; fluorescent antibody technique; genes; glycosaminoglycans; histology; in vivo studies; liver; phenotype; quercetin; rats; scaffolding proteins; secretion; signal transduction; silk proteins; staining; tissue repair
Abstract:: ... Due to the poor repair ability of cartilage tissue, regenerative medicine still faces great challenges in the repair of large articular cartilage defects. Quercetin is widely applied as a traditional Chinese medicine in tissue regeneration including liver, bone and skin tissues. However, the evidence for its effects and internal mechanisms for cartilage regeneration are limited. In the present stu ...
DOI:: 10.1016/S1875-5364(20)60036-X
: https://dx.doi.org/10.1016/S1875-5364(20)60036-X

96. Effects of crowding on the three main proteolytic mechanisms of skeletal muscle in rainbow trout (Oncorhynchus mykiss)

Author:: Valenzuela, Cristián A.; Ponce, Claudia; Zuloaga, Rodrigo; González, Pamela; Avendaño-Herrera, Ruben; Valdés, Juan A.; Molina, Alfredo
Source:: BMC veterinary research 2020 v.16 no.1 pp. 294
ISSN:: 1746-6148
Subject:: Oncorhynchus mykiss; Western blotting; animal welfare; calpain; calpastatin; cortisol; descriptive studies; energy; enzyme immunoassays; fish; fish culture; freshwater; genes; glucose; juveniles; muscle physiology; muscles; plant density; protein degradation; proteolysis; skeletal muscle; stocking rate; supply; veterinary medicine; weight loss; Chile
Abstract:: ... BACKGROUND: Skeletal muscle is one of the tissues most affected by stress conditions. The protein degradation in this tissue is vital for the supply of energy mediated by different proteolytic pathways such as the ubiquitin-proteasome (UPS), autophagy-lysosome (ALS) and the calpain/calpastatin system (CCS). Nevertheless, the regulation of this proteolytic axis under stress conditions is not yet co ...
DOI:: 10.1186/s12917-020-02518-w
: https://dx.doi.org/10.1186/s12917-020-02518-w

97. Effects of diclazuril on the expression of enolase in second-generation merozoites of Eimeria tenella

Author:: Zhou, Bian-hua; Ding, Hai-yan; Yang, Jing-yun; Chai, Jun; Guo, Hong-wei; Wang, Hong-wei
Source:: Poultry science 2020 v.99 no.12 pp. 6402-6409
ISSN:: 0032-5791
Subject:: Eimeria tenella; Escherichia coli; Western blotting; antibodies; cecum; chicken industry; chickens; cytoplasm; diclazuril; financial economics; fluorescent antibody technique; glycolysis; merozoites; messenger RNA; parasites; phosphopyruvate hydratase; plasmids; protein synthesis; quantitative polymerase chain reaction
Abstract:: ... Eimeria tenella is an obligate intracellular parasite of the chicken cecum; it brings huge economic loss to the chicken industry. Enolase is a multifunctional glycolytic enzyme involved in many processes of parasites, such as infection and migration. In this study, the effect of diclazuril on the expression of enolase in second-generation merozoites of E. tenella (EtENO) was reported. The prokaryo ...
DOI:: 10.1016/j.psj.2020.09.068
: https://dx.doi.org/10.1016/j.psj.2020.09.068

98. Effects of 17β-Estradiol on growth-related genes expression in female and male spotted scat (Scatophagus argus)

Author:: Ru, Xiao-Ying; Shi, Hong-Juan; Wang, Tuo; Liu, Qian-Qing; Jiang, Dong-Neng; Peng, Yu-Hao; Huang, Yang; Zhu, Chun-Hua; Li, Shui-Sheng; Dong, Ran-Ran; Li, Guang-Li
Source:: Comparative biochemistry and physiology 2020 v.250 pp. 110492
ISSN:: 1096-4959
Subject:: Scatophagus argus; Western blotting; animal ovaries; dimorphism; estradiol; feces; females; gene expression; gene expression regulation; hypothalamus; immunohistochemistry; liver; males; mariculture; messenger RNA; molecular weight; pituitary gland; polyclonal antibodies; protein synthesis; quantitative polymerase chain reaction; somatotropin; testes; China
Abstract:: ... Growth hormone (GH) is the most important endocrine factor to regulate somatic growth. Spotted scat (Scatophagus argus) is a famous marine aquaculture species in China with a typical sexual growth dimorphism in which females grow faster and larger than males. In this study, gh messenger RNA (gh mRNA) and GH protein expression were examined in the pituitary glands of female and male spotted scat. B ...
DOI:: 10.1016/j.cbpb.2020.110492
: https://dx.doi.org/10.1016/j.cbpb.2020.110492

99. Enhanced tolerance to Phytophthora root and stem rot by over-expression of the plant antimicrobial peptide CaAMP1 gene in soybean

Author:: Niu, Lu; Zhong, Xiaofang; Zhang, Yuanyu; Yang, Jing; Xing, Guojie; Li, Haiyun; Liu, Dongbo; Ma, Rui; Dong, Yingshan; Yang, Xiangdong
Source:: BMC genetics 2020 v.21 no.1 pp. 68
ISSN:: 1471-2156
Subject:: Arabidopsis; Capsicum annuum; Glycine max; Phytophthora sojae; Western blotting; Xanthomonas vesicatoria; animals; antimicrobial peptides; antimicrobial proteins; bacterial diseases of plants; fungi; gene expression regulation; gene overexpression; genetic transformation; leaves; plant pathogenic bacteria; resistance genes; signal transduction; soybeans; stem rot; transgenic plants; translation (genetics)
Abstract:: ... BACKGROUND: Antimicrobial peptides play important roles in both plant and animal defense systems. Moreover, over-expression of CaAMP1 (Capsicum annuum antimicrobial protein 1), an antimicrobial protein gene isolated from C. annuum leaves infected with Xanthomonas campestris pv. vesicatoria, confers broad-spectrum resistance to hemibiotrophic bacterial and necrotrophic fungal pathogens in Arabidops ...
DOI:: 10.1186/s12863-020-00872-0
: https://dx.doi.org/10.1186/s12863-020-00872-0

100. Experimental immunization of mice with a recombinant bovine enterovirus vaccine expressing BVDV E0 protein elicits a long-lasting serologic response

Author:: Ren, Xiao; Zhang, Shan; Gao, Xintao; Guo, Xiaoyu; Xin, Ting; Zhu, Hongfei; Jia, Hong; Hou, Shaohua
Source:: Virology journal 2020 v.17 no.1 pp. 88
ISSN:: 1743-422X
Subject:: Bovine viral diarrhea virus 1; Western blotting; animal viruses; antibodies; antibody formation; cattle industry; complementary DNA; financial economics; fluorescent antibody technique; genes; genetic stability; host range; immunization; mice; plasmids; polymerase chain reaction; proteinases; transfection; vector vaccines; viral vaccines; virulence; viruses
Abstract:: ... BACKGROUND: Bovine viral diarrhea virus (BVDV) is a cause of substantial economic loss to the cattle industry worldwide, and there are currently no effective treatment or preventive measures. Bovine enterovirus (BEV) has a broad host range with low virulence and is a good candidate as a viral vaccine vector. In this study, we explored new insertion sites for the expression of exogenous genes in BE ...
DOI:: 10.1186/s12985-020-01338-6
: https://dx.doi.org/10.1186/s12985-020-01338-6

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1. Development and evaluation of recombinant GRA8 protein for the serodiagnosis of Toxoplasma gondii infection in goats

2. Identification of a universal antigen epitope of influenza A virus using peptide microarray

3. Filamin A is required for somatostatin receptor type 5 expression and pasireotide-mediated signaling in pituitary corticotroph tumor cells

4. A lipid-soluble extract of Pinellia pedatisecta Schott orchestrates intratumoral dendritic cell-driven immune activation through SOCS1 signaling in cervical cancer

5. Activation of MAPK signaling in response to nitrogen deficiency in Ulva prolifera (Chlorophyta)

6. Characterisation of novel regulatory sequences compatible with modular assembly in the diatom Phaeodactylum tricornutum

7. Validation of a monoclonal antibody directed against the human sphingosine 1-phosphate receptor type 1

8. Ozone exposure promotes pyroptosis in rat lungs via the TLR2/4-NF-κB-NLRP3 signaling pathway

9. Identification of lipidomic responses to GCRV infection in Ctenopharyngodon idellus kidney (CIK) cells

10. Development and characterization of novel cell lines from kidney and eye of rainbow trout, Oncorhynchus mykiss for virological studies

11. Development and characterization of polyclonal antibodies against subtype specific vitellogenin of the dojo loach, Misgurnus anguillicaudatus

12. Gli activation by the estrogen receptor in breast cancer cells: Regulation of cancer cell growth by Gli3

13. Polystyrene microplastics cause granulosa cells apoptosis and fibrosis in ovary through oxidative stress in rats

14. Anti-depressant effect of Zhi-zi-chi decoction on CUMS mice and elucidation of its signaling pathway

15. Nanoparticle delivery improves the pharmacokinetic properties of cyclic dinucleotide STING agonists to open a therapeutic window for intravenous administration

16. Antidiabetic activity of a Flavonoid-Rich extract from flowers of Wisteria sinensis in type 2 diabetic mice via activation of the IRS-1/PI3K/Akt/GLUT4 pathway

17. Grifola frondosa GF5000 improves insulin resistance by modulation the composition of gut microbiota in diabetic rats

18. Mitochondrial dynamics in Angiostrongylus cantonensis-infected mouse brain

19. Apigenin attenuates TGF-β1-stimulated cardiac fibroblast differentiation and extracellular matrix production by targeting miR-155-5p/c-Ski/Smad pathway

20. Fucoxanthin extracted from Laminaria Japonica inhibits metastasis and enhances the sensitivity of lung cancer to Gefitinib

21. Myosin light chain kinase mediates intestinal barrier dysfunction following simulated microgravity based on proteomic strategy

22. Quantitative proteomics of cerebrospinal fluid using tandem mass tags in dogs with recurrent epileptic seizures

23. Saliva-omics in plasma cell disorders- Proof of concept and potential as a non-invasive tool for monitoring disease burden

24. Ameliorative effects of Qingganjiuwei powder, a traditional Mongolian medicine, against CCl4-induced liver fibrosis in rats

25. Berberine inhibits intestinal epithelial barrier dysfunction in colon caused by peritoneal dialysis fluid by improving cell migration

26. Effect of weimaining on apoptosis and Caspase-3 expression in a breast cancer mouse model

27. Ethanol extract of Liriodendron chinense (Hemsl.) Sarg barks attenuates hyperuricemic nephropathy by inhibiting renal fibrosis and inflammation in mice

28. Kanglaite enhances the efficacy of cisplatin in suppression of hepatocellular carcinoma via inhibiting CKLF1 mediated NF-κB pathway and regulating transporter mediated drug efflux

29. Kuijieling decoction suppresses NLRP3-Mediated pyroptosis to alleviate inflammation and experimental colitis in vivo and in vitro

30. Lophatherum gracile Brongn. attenuates neutrophilic inflammation through inhibition of JNK and calcium

31. Repair function of essential oil from Crocodylus Siamensis (Schneider, 1801) on the burn wound healing via up-regulated growth factor expression and anti-inflammatory effect

32. San Wu Huangqin decoction regulates inflammation and immune dysfunction induced by influenza virus by regulating the NF-κB signaling pathway in H1N1-infected mice

33. TMT-based proteomics analysis reveals the efficacy of jiangzhuo formula in improving the lipid profiles of dyslipidemia rats

34. Xiaoyaosan decoction alleviated rat liver fibrosis via the TGFβ/Smad and Akt/FoxO3 signaling pathways based on network pharmacology analysis

35. Proteomic analysis of bEnd.3 cells infected with wild-type and stk-deficient strains of Streptococcus suis serotype 2 reveals protein and pathway regulation

36. Diallyl disulfide improves lipid metabolism by inhibiting PCSK9 expression and increasing LDL uptake via PI3K/Akt-SREBP2 pathway in HepG2 cells

37. An ecofriendly synthesized gold nanoparticles induces cytotoxicity via apoptosis in HepG2 cells

38. Cardiac glycosides from the roots of Streblus asper Lour. and their apoptosis-inducing activities in A549 cells

39. D‐galactose‐induced toxicity associated senescence mitigated by alpinate oxyphyllae fructus fortified adipose‐derived mesenchymal stem cells

40. Prolactin neuroprotective action against excitotoxic insult in the hippocampus of male mice

41. Rumen-bypassed tributyrin alleviates heat stress by reducing the inflammatory responses of immune cells

42. The entomotoxin Jack Bean Urease changes cathepsin D activity in nymphs of the hematophagous insect Dipetalogaster maxima (Hemiptera: Reduviidae)

43. The p53/RMRP/miR122 signaling loop promotes epithelial-mesenchymal transition during the development of silica-induced lung fibrosis by activating the notch pathway

44. The relationship between the mTOR signaling pathway and ovarian aging in peak-phase and late-phase laying hens

45. Chronic ethanol exposure induces neuroinflammation in H4 cells through TLR3 / NF-κB pathway and anxiety-like behavior in male C57BL/6 mice

46. Quantitative comparative analysis uncovered the role of E2 in Nile tilapia GSD + TE

47. Phoenix dactylifera L. Seed Extract Exhibits Antioxidant Effects and Attenuates Melanogenesis in B16F10 Murine Melanoma Cells by Downregulating PKA Signaling

48. Unconventional Secretion of Nigerolysins A from Aspergillus Species

49. Fluorescence Detection of Type III Secretion Using a Glu-CyFur Reporter System in Citrobacter rodentium

50. An isoflavone extract from soybean cake suppresses 2,4-dinitrochlorobenzene-induced contact dermatitis

51. Danlou tablet inhibits the inflammatory reaction of high-fat diet-induced atherosclerosis in ApoE knockout mice with myocardial ischemia via the NF-κB signaling pathway

52. Holistic quality evaluation of Qingwen Baidu Decoction and its anti-inflammatory effects

53. In vitro cultured calculus bovis attenuates cerebral ischaemia-reperfusion injury by inhibiting neuronal apoptosis and protecting mitochondrial function in rats

54. Pharmacoproteomics reveals the mechanism of Chinese dragon's blood in regulating the RSK/TSC2/mTOR/ribosome pathway in alleviation of DSS-induced acute ulcerative colitis

55. Shenqi Yizhi granules protect hippocampus of AD transgenic mice by modulating on multiple pathological processes

56. Systematic biological and proteomics strategies to explore the regulation mechanism of Shoutai Wan on recurrent spontaneous Abortion's biological network

57. Wild simulated ginseng activates mouse macrophage, RAW264.7 cells through TRL2/4-dependent activation of MAPK, NF-κB and PI3K/AKT pathways

58. A practical guide for analysis of histone post-translational modifications by mass spectrometry: Best practices and pitfalls

59. Modulation of peroxynitrite produced via mitochondrial nitric oxide synthesis during Ca2+ and succinate-induced oxidative stress in cardiac isolated mitochondria

60. Study on the attenuated effect of Ginkgolide B on ferroptosis in high fat diet induced nonalcoholic fatty liver disease

61. Activation of the Nrf2/HO-1 pathway by curcumin inhibits oxidative stress in human nasal fibroblasts exposed to urban particulate matter

62. Alisol A-24-acetate promotes glucose uptake via activation of AMPK in C2C12 myotubes

63. Ampicillin triggers the release of Pal in toxic vesicles from Escherichia coli

64. Angiostrongylus cantonensis Galectin-1 interacts with Annexin A2 to impair the viability of macrophages via activating JNK pathway

65. A novel protein chip for simultaneous detection of antibodies against four epidemic swine viruses in China

66. Astragalin alleviates cerebral ischemia-reperfusion injury by improving anti-oxidant and anti-inflammatory activities and inhibiting apoptosis pathway in rats

67. Betaine attenuates LPS-induced downregulation of Occludin and Claudin-1 and restores intestinal barrier function

68. Betaine attenuates LPS-induced downregulation of Occludin and Claudin-1 and restores intestinal barrier function

69. Bovine adapted transmissible mink encephalopathy is similar to L-BSE after passage through sheep with the VRQ/VRQ genotype but not VRQ/ARQ

70. Buyang Huanwu Tang inhibits cellular epithelial-to-mesenchymal transition by inhibiting TGF-β1 activation of PI3K/Akt signaling pathway in pulmonary fibrosis model in vitro