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- Author:
- Jirapattharasate, Charoonluk; Udonsom, Ruenruetai; Prachasuphap, Apichai; Jongpitisub, Kodcharad; Dhepakson, Panadda
- Source:
- BMC veterinary research 2021 v.17 no.1 pp. 27
- ISSN:
- 1746-6148
- Subject:
- Escherichia coli; Toxoplasma gondii; Western blotting; antibodies; antigens; blood serum; cell culture; enzyme-linked immunosorbent assay; extraction; genomics; goats; humans; infection; laboratory animals; latex agglutination test; molecular weight; plasmids; polyacrylamide gel electrophoresis; protein synthesis; recombinant proteins; sampling; serodiagnosis; synthesis; synthetic genes; tachyzoites; toxoplasmosis; veterinary medicine
- Abstract:
- ... BACKGROUND: The development of sensitive and specific methods for detecting Toxoplasma gondii infection is critical for preventing and controlling toxoplasmosis in humans and other animals. Recently, various recombinant proteins have been used in serological tests for diagnosing toxoplasmosis. The production of these antigens is associated with live tachyzoites obtained from cell cultures or labor ...
- DOI:
- 10.1186/s12917-020-02719-3
- https://dx.doi.org/10.1186/s12917-020-02719-3
- Author:
- Wang, Qiuxia; Sun, Zhihao; Li, Jingzhi; Qin, Tao; Ma, Hongwei; Chen, Sujuan; Peng, Daxin; Liu, Xiufan
- Source:
- BMC veterinary research 2021 v.17 no.1 pp. 22
- ISSN:
- 1746-6148
- Subject:
- Anas platyrhynchos; CD4-positive T-lymphocytes; Influenza A virus; Western blotting; amino acid substitution; amino acids; antiserum; blood serum; databases; detection; epitopes; hemagglutinins; interleukin-2; peptide microarrays; silica gel; surface proteins; vaccine development; veterinary medicine; viruses
- Abstract:
- ... BACKGROUND: Hemagglutinin is a major surface protein in influenza A virus (IAV), and HA2 is relative conserved among different IAVs. It will be meaningful to identify broad-spectrum epitopes based on the HA2 protein. RESULTS: Overlapping peptides of the HA2 protein of the H5N1 IAV A/Mallard/Huadong/S/2005 were synthesized and loaded on modified silica gel film to form a microarray, and antisera ag ...
- DOI:
- 10.1186/s12917-020-02725-5
- https://dx.doi.org/10.1186/s12917-020-02725-5
- Author:
- Treppiedi, Donatella; Di Muro, Genesio; Mangili, Federica; Catalano, Rosa; Giardino, Elena; Barbieri, Anna Maria; Locatelli, Marco; Arosio, Maura; Spada, Anna; Peverelli, Erika; Mantovani, Giovanna
- Source:
- Molecular and cellular endocrinology 2021 v.524 pp. 111159
- ISSN:
- 0303-7207
- Subject:
- Western blotting; apoptosis; cell proliferation; corticotropin; cytoskeleton; filamin; hormone secretion; humans; mice; neoplasm cells; neoplasms; somatostatin; somatostatin receptors
- Abstract:
- ... Somatostatin receptor type 5 (SST5) represents the main pharmacological target in the treatment of adrenocorticotroph hormone (ACTH)-secreting tumors. However, molecular predictors of responsiveness to pasireotide require further investigation. The cytoskeleton protein filamin A (FLNA) modulates the responsiveness to somatostatin analogs (SSA) treatment in other types of pituitary tumors by regula ...
- DOI:
- 10.1016/j.mce.2021.111159
- https://dx.doi.org/10.1016/j.mce.2021.111159
- Author:
- Wang, Yumeng; Lu, Chong; Huang, Haixia; Yao, Sheng; Xu, Congjian; Ye, Yang; Gui, Suiqi; Li, Guiling
- Source:
- Journal of ethnopharmacology 2021 v.267 pp. 112837
- ISSN:
- 0378-8741
- Subject:
- Pinellia; Western blotting; apoptosis; biomarkers; bone marrow; dendritic cells; drugs; flow cytometry; gene silencing; genetic markers; humans; interleukin-12; medicinal plants; mice; models; neoplasm cells; non-specific protein-tyrosine kinase; phenotype; phosphorylation; quantitative polymerase chain reaction; traditional medicine; uterine cervical neoplasms
- Abstract:
- ... Pinellia pedatisecta Schott extract (PE) is generated from Pinellia pedatisecta Schott, a traditional Chinese medicinal plant. PE suppresses cervical tumor growth and exhibits effects on dendritic cells (DCs) that lead to modulation of antitumor CD4⁺ and CD8⁺ responses.To explore the underlying mechanisms by which PE modulates tumor-associated dendritic cell (TADC) activation and function.DCs and ...
- DOI:
- 10.1016/j.jep.2020.112837
- https://dx.doi.org/10.1016/j.jep.2020.112837
- Author:
- Yang, Juanjuan; Yin, Yi; Yu, Dachun; He, Lihong; Shen, Songdong
- Source:
- Algal research 2021 v.53 pp. 102153
- ISSN:
- 2211-9264
- Subject:
- Ulva prolifera; Western blotting; algal blooms; apoptosis; assays; fluorescence; glutamine; macroalgae; mortality; nitrate reductase; nitrite reductase; nitrogen; protoplasts; research; staining
- Abstract:
- ... Ulva prolifera (Ulvophyceae, Chlorophyta), one of the main seaweeds species that cause “green tides”, suffers changes in nitrogen levels during drifting. However, the mechanisms of how this alga resist to nitrogen deficiency remains unclear. Transcripts encoding nitrate reductase, nitrite reductase, and glutamine synthase involved in nitrogen assimilation were amplified by RT-PCR, suggesting that ...
- DOI:
- 10.1016/j.algal.2020.102153
- https://dx.doi.org/10.1016/j.algal.2020.102153
- Author:
- Windhagauer, Matthias; Abbriano, Raffaela M.; Ashworth, Justin; Barolo, Lorenzo; Jaramillo-Madrid, Ana Cristina; Pernice, Mathieu; Doblin, Martina A.
- Source:
- Algal research 2021 v.53 pp. 102159
- ISSN:
- 2211-9264
- Subject:
- Phaeodactylum tricornutum; Western blotting; algae; databases; flow cytometry; fluorescence; gene expression; genetic engineering; genome; heterologous gene expression; metabolic engineering; models; nitrogen; photoperiod; plasmids; promoter regions; protein content; research; synthetic biology; transcriptomics
- Abstract:
- ... Phaeodactylum tricornutum is a model pennate diatom for molecular studies due to its sequenced genome, genetic tractability, and capacity to produce a myriad of compounds of biotechnological interest. While tools for genetic engineering and heterologous gene expression have been developed for this species, the available set of characterised promoters remains limited. A diverse and well-understood ...
- DOI:
- 10.1016/j.algal.2020.102159
- https://dx.doi.org/10.1016/j.algal.2020.102159
- Author:
- Thuy, Andreas V.; Jeya Paul, Jefri; Weigel, Cynthia; Ziegler, Anke C.; Guntinas-Lichius, Orlando; Gräler, Markus H.
- Source:
- Journal of immunological methods 2021 v.490 pp. 112953
- ISSN:
- 0022-1759
- Subject:
- B-lymphocytes; Western blotting; agonists; antagonists; antibody specificity; cell lines; epitopes; flow cytometry; human umbilical vein endothelial cells; humans; immunoglobulin M; mice; monoclonal antibodies; precipitin tests; sphingosine; staining; tonsils
- Abstract:
- ... The sphingosine 1-phosphate receptor type 1 (S1PR1) has several important functions, including stabilizing endothelial barrier and maintaining lymphocyte circulation. These functions are critically dependent on the regulation of S1PR1 cell surface expression. Currently available antibodies against human S1PR1 are not able to pick up cell surface expression on living cells by flow cytometry due to ...
- DOI:
- 10.1016/j.jim.2020.112953
- https://dx.doi.org/10.1016/j.jim.2020.112953
- Author:
- Tian, Lei; Yan, Jun; Li, Kang; Zhang, Wei; Lin, Bencheng; Lai, Wenqing; Bian, Liping; Liu, Huanliang; Xi, Zhuge; Liu, Xiaohua
- Source:
- Toxicology 2021 v.450 pp. 152668
- ISSN:
- 0300-483X
- Subject:
- Toll-like receptors; Western blotting; air pollutants; animal experimentation; assays; blood; caspase-1; edema; epithelial cells; females; histopathology; hyaluronic acid; inflammasomes; inflammation; lungs; males; nucleoproteins; ozone; pyroptosis; rats; respiratory tract diseases; signal transduction; toxicology; transcription factor NF-kappa B
- Abstract:
- ... Ozone has become a major air pollutant in recent years, which leading to a variety of lung diseases. This study aimed to explore the mechanisms of pyroptosis and related signaling pathways in ozone-induced lung injury.We exposed 120 Wistar rats to ozone, 20 in each group (half male and half female). Ozone exposure concentrations were 0, 0.12, 0.5, 1.0, 2.0 and 4.0 ppm for 6 h. At the same time, we ...
- DOI:
- 10.1016/j.tox.2020.152668
- https://dx.doi.org/10.1016/j.tox.2020.152668
- Author:
- Zhang, Xing; Huang, Jingcai; Shen, Zeen; Wang, Yan; Wang, Yujun; Gong, Chengliang; Hu, Xiaolong; Wang, Chonglong
- Source:
- Aquaculture 2021 v.533 pp. 736106
- ISSN:
- 0044-8486
- Subject:
- Ctenopharyngodon idella; Reoviridae; Western blotting; aquaculture; autophagy; biochemical pathways; droplets; financial economics; fish diseases; immune response; kidney cells; kidneys; lipid composition; lipid metabolism; lipidomics; pathogens; staining; triacylglycerols; virus replication; viruses
- Abstract:
- ... Grass carp reovirus (GCRV) is a causing pathogen of grass carp hemorrhagic disease, whose outbreak often brings out a huge economic loss in aquaculture filed. Cellular lipids play crucial roles in many biological processes, such as virus replication, maturation and transmission. Presently, it remains to elucidate the roles of these lipids in the GCRV infection. In this study, Here, a lipidomic app ...
- DOI:
- 10.1016/j.aquaculture.2020.736106
- https://dx.doi.org/10.1016/j.aquaculture.2020.736106
- Author:
- Suryakodi, S.; Majeed, S. Abdul; Taju, G.; Vimal, S.; Sivakumar, S.; Ahmed, A. Nafeez; Shah, Feroz Ahmad; Bhat, Saqib Ahmad; Sarma, Dandadhar; Begum, Ajima; Hameed, A.S. Sahul
- Source:
- Aquaculture 2021 v.532 pp. 736027
- ISSN:
- 0044-8486
- Subject:
- Cyprinid herpesvirus 2; Nodaviridae; Oncorhynchus mykiss; Tilapia lake virus; Western blotting; cell growth; cell lines; cryopreservation; enzyme-linked immunosorbent assay; epithelium; eyes; fibronectins; fish; fluorescent antibody technique; kidneys; reverse transcriptase polymerase chain reaction; sequence analysis; spleen; viruses; India
- Abstract:
- ... Two novel cell lines were developed from eye and kidney of rainbow trout, Oncorhynchus mykiss in addition to another cell line from spleen. All these cell lines were characterized and cryopreserved successfully at different passage levels. The cells of eye, kidney and spleen were sub-cultured continuously at an interval of 2–3 days and attained the passage level of 112, 106 and 112, respectively o ...
- DOI:
- 10.1016/j.aquaculture.2020.736027
- https://dx.doi.org/10.1016/j.aquaculture.2020.736027
- Author:
- Wang, Weilong; Lian, Qingping; Chen, Yuange; Hiramatsu, Naoshi; Wu, Meiqin
- Source:
- Aquaculture 2021 v.532 pp. 736089
- ISSN:
- 0044-8486
- Subject:
- Misgurnus anguillicaudatus; Western blotting; antiserum; aquatic environment; biomarkers; chemiluminescence; estradiol; estrogenic properties; fish; genes; lowest observed effect level; males; polyclonal antibodies; vitellogenin
- Abstract:
- ... Fish vitellogenin (Vtg) has become an important biomarker for assessing the estrogenic potency of chemicals and the exposure of animals to estrogenic contaminants present in aquatic environments. In the current study, the polyclonal antibodies against subtype-specific Vtgs of the dojo loach (Misgurnus anguillicaudatus) were developed and identified by gene recombination and expression. Immuno-bioc ...
- DOI:
- 10.1016/j.aquaculture.2020.736089
- https://dx.doi.org/10.1016/j.aquaculture.2020.736089
- Author:
- Massah, Shabnam; Foo, Jane; Li, Na; Truong, Sarah; Nouri, Mannan; Xie, Lishi; Prins, Gail S.; Buttyan, Ralph
- Source:
- Molecular and cellular endocrinology 2021 v.522 pp. 111136
- ISSN:
- 0303-7207
- Subject:
- Western blotting; androgen receptors; breast neoplasms; cell growth; coprecipitation; estradiol; estrogen receptors; extracts; genes; ligands; neoplasm cells; precipitin tests; prostatic neoplasms; proteolysis
- Abstract:
- ... Gli is an oncogenic transcription factor family thought to be involved in breast cancer (BrCa) cell growth. Gli activity is regulated by a post-translational proteolytic process that is suppressed by Hedgehog signaling. In prostate cancer cells, however, Gli activation is mediated by an interaction of active androgen receptor proteins with Gli3 that stabilizes Gli3 in its un-proteolyzed form. Here ...
- DOI:
- 10.1016/j.mce.2020.111136
- https://dx.doi.org/10.1016/j.mce.2020.111136
- Author:
- An, Ru; Wang, Xifeng; Yang, Long; Zhang, Jinjin; Wang, Nana; Xu, Feibo; Hou, Yun; Zhang, Hongqin; Zhang, Lianshuang
- Source:
- Toxicology 2021 v.449 pp. 152665
- ISSN:
- 0300-483X
- Subject:
- Western blotting; acetylcysteine; actin; anti-Mullerian hormone; apoptosis; blood; enzyme-linked immunosorbent assay; females; fibronectins; fibrosis; flow cytometry; granulosa cells; immunohistochemistry; microplastics; muscles; oxidative stress; pollutants; polystyrenes; rats; reactive oxygen species; reproductive toxicology; signal transduction; staining; testing
- Abstract:
- ... Microplastics (MPs) are receiving increased attention as a harmful environmental pollutant. Studies have investigated that MPs have reproductive toxicity, but the mechanism is little known. Here, we aimed to investigate the effects of polystyrene microplastics (PS-MPs) on ovary in rats and the underlying molecular mechanisms. in vivo, thirty-two female Wistar rats were exposed to 0.5 μm PS-MPs at ...
- DOI:
- 10.1016/j.tox.2020.152665
- https://dx.doi.org/10.1016/j.tox.2020.152665
14. Anti-depressant effect of Zhi-zi-chi decoction on CUMS mice and elucidation of its signaling pathway
- Author:
- Chai, Chuan; Jin, Bo; Yan, Yinyu; Yuan, Qi; Wen, Hongmei; Tao, Weiwei; Cui, Xiaobing; Shan, Chenxiao; Yu, Sheng
- Source:
- Journal of ethnopharmacology 2021 v.266 pp. 113283
- ISSN:
- 0378-8741
- Subject:
- Oriental traditional medicine; Western blotting; amino acid metabolism; antidepressants; arginine; aspartic acid; biochemical pathways; biomarkers; blood serum; body weight; cAMP-dependent protein kinase; carbohydrate metabolism; fluoxetine; forced swimming test; gamma-aminobutyric acid; glutamic acid; glutamine; hippocampus; lipid metabolism; males; metabolites; metabolomics; mice; models; pharmacokinetics; phosphatidylcholines; proline; protein synthesis; signal transduction; sucrose; swimming; tail suspension test
- Abstract:
- ... Zhi-zi-chi decoction (ZZCD) is used for treating depression as an effectively traditional Chinese medicine. Until now, studies on pharmacological research of ZZCD have mostly been centered in pharmacokinetic level. Little was known about its pharmacological mechanism of relieving depression.This study was to evaluate the effect of ZZCD on relieving depression via behavioral tests, serum metabolomi ...
- DOI:
- 10.1016/j.jep.2020.113283
- https://dx.doi.org/10.1016/j.jep.2020.113283
- Author:
- Wehbe, Mohamed; Wang-Bishop, Lihong; Becker, Kyle W.; Shae, Daniel; Baljon, Jessalyn J.; He, Xinyi; Christov, Plamen; Boyd, Kelli L.; Balko, Justin M.; Wilson, John T.
- Source:
- Journal of controlled release 2021 v.330 pp. 1118-1129
- ISSN:
- 0168-3659
- Subject:
- CD8-positive T-lymphocytes; Western blotting; adenocarcinoma; agonists; antibodies; breast neoplasms; encapsulation; gene expression regulation; genes; guanosine; half life; immunosuppression; interferons; intravenous injection; ligands; liver; medicinal properties; melanoma; models; monitoring; nanocapsules; nanocarriers; nanomedicine; nanoparticles; pharmacokinetics; spleen
- Abstract:
- ... The stimulator of interferon genes (STING) pathway plays an important role in the immune surveillance of cancer and, accordingly, agonists of STING signaling have recently emerged as promising therapeutics for remodeling of the immunosuppressive tumor microenvironment (TME) and enhancing response rates to immune checkpoint inhibitors. 2′3′-cyclic guanosine monophosphate–adenosine monophosphate (2′ ...
- DOI:
- 10.1016/j.jconrel.2020.11.017
- https://dx.doi.org/10.1016/j.jconrel.2020.11.017
- Author:
- Huang, Yun; Zhou, Tongxi; Zhang, Yirui; Huang, Huiqi; Ma, Yuanren; Wu, Chaoqun; Wang, Qiang; Lin, Qinxiong; Yang, Xinzhou; Pang, Kejian
- Source:
- Journal of functional foods 2021 v.77 pp. 104338
- ISSN:
- 1756-4646
- Subject:
- Western blotting; Wisteria sinensis; adipocytes; fatty liver; flowers; functional foods; glucose; glucose transporters; glycemic effect; histopathology; hyperglycemia; hyperinsulinemia; hyperlipidemia; hypertrophy; insulin; insulin resistance; islets of Langerhans; metabolism; mice; noninsulin-dependent diabetes mellitus; phosphatidylinositol 3-kinase; phosphorylation; plasma membrane; serine; tissues
- Abstract:
- ... The present study aimed to investigate anti-diabetic activities of a flavonoid-rich extract from Wisteria sinensis (WS-FRE). In vitro, WS-FRE significantly promote the translocation of GLUT4 (Glucose transporter 4) to the plasma membrane. Mechanisms analysis revealed that WS-FRE enhanced glucose uptake via activation of Akt and GLUT4. In vivo, 4-week treatment of WS-FRE alleviated the typical symp ...
- DOI:
- 10.1016/j.jff.2020.104338
- https://dx.doi.org/10.1016/j.jff.2020.104338
- Author:
- Xiao, Chun; Jiao, Chunwei; Xie, Yizhen; Ye, Linhui; Li, Qianqing; Wu, Qingping
- Source:
- Journal of functional foods 2021 v.77 pp. 104313
- ISSN:
- 1756-4646
- Subject:
- Grifola frondosa; Western blotting; area; blood glucose; blood lipids; dysbiosis; epididymis; functional foods; genes; glucose tolerance tests; glycemic effect; inflammation; insulin resistance; intestinal microorganisms; intestines; protein content; rats; white adipose tissue
- Abstract:
- ... The aim was to investigate whether Grifola frondosa fraction (Mw > 5000D) (GF5000) could restore the gut microbiota dysbiosis to improves insulin resistance in diabetic rats. Oral glucose tolerance test and serum biochemical parameter were assessed of the hypoglycemic activity of GF5000 (112.5 and 675 mg/kg/d, i.g.) in diabetic rats for 48 d. The mRNA and protein levels of inflammatory markers in ...
- DOI:
- 10.1016/j.jff.2020.104313
- https://dx.doi.org/10.1016/j.jff.2020.104313
- Author:
- Lam, Ho Yin Pekkle; Chen, Cheng-Chi; Chen, Tina Tu-Wen; Chang, Kai-Chih; Wu, Wen-Jui; Yang, Ting-Hua; Liang, Ting-Ruei; Cheng, Po-Ching; Peng, Shih-Yi
- Source:
- Parasitology international 2021 v.80 pp. 102231
- ISSN:
- 1383-5769
- Subject:
- Angiostrongylus; B-cell lymphoma; Parastrongylus cantonensis; Western blotting; apoptosis; biogenesis; brain; caspases; dynamics; exhibitions; infection; inflammation; lead; meningoencephalitis; mice; mitochondria; molecular biology; nucleotidyltransferases; occurrence; parasites; parasitology; pathogenesis; polymerase chain reaction; staining; transmission electron microscopy
- Abstract:
- ... Angiostrongylus cantonensis is one of the most widespread parasites causing central nervous system (CNS) diseases in mammals. Since the mitochondrion is an essential cell organelle responsible for both physiological and pathological processes, its dysfunction might lead to inflammation and multiple disorders. In this study we aimed to investigate the changes in mitochondrial dynamics that occur in ...
- DOI:
- 10.1016/j.parint.2020.102231
- https://dx.doi.org/10.1016/j.parint.2020.102231
- Author:
- Wang, Feng; Fan, Ke; Zhao, Ying; Xie, Mei-Lin
- Source:
- Journal of ethnopharmacology 2021 v.265 pp. 113195
- ISSN:
- 0378-8741
- Subject:
- 3' untranslated regions; Matricaria; Western blotting; actin; apigenin; cardiovascular diseases; chamomile; collagen; enzyme activity; extracellular matrix; fibroblasts; luciferase; muscles; mutants; quantitative polymerase chain reaction; reporter genes; synergism; traditional medicine; transfection; transforming growth factor beta 1
- Abstract:
- ... Apigenin is a natural flavonoid compound present in chamomile (Matricaia chamomilla L.) from the Asteraceae family, which is used in the treatment of cardiovascular diseases by traditional healers, but its effects on differentiation and extracellular matrix (ECM) production of cardiac fibroblasts (CFs) induced by transforming growth factor beta 1 (TGF-β1) are poorly understood.This study aimed to ...
- DOI:
- 10.1016/j.jep.2020.113195
- https://dx.doi.org/10.1016/j.jep.2020.113195
- Author:
- Ming, Jia Xiong; Wang, Zhao Cong; Huang, Yi; Ohishi, Hifumi; Wu, Rong Ji; Shao, Yan; Wang, Hui; Qin, Ming Yang; Wu, Ze Liang; Li, Yi Yong; Chang Zhou, Shun; Chen, Hui; Liu, Hui; Xu, Rong
- Source:
- Journal of ethnopharmacology 2021 v.265 pp. 113302
- ISSN:
- 0378-8741
- Subject:
- Oriental traditional medicine; RNA interference; Saccharina japonica; Western blotting; antineoplastic activity; cadherins; cell lines; cell proliferation; cell viability; drugs; fibronectins; fluorescent antibody technique; gene expression; human diseases; immunohistochemistry; lung neoplasms; lungs; metastasis; models; neoplasm cells; phenotype; phosphatidylinositol 3-kinase; staining; tissue repair; transcription factor NF-kappa B; xenotransplantation
- Abstract:
- ... Laminaria japonica, a brown seaweed, has been used in Traditional Chinese Medicine (TCM) to treat a variety of diseases including lung cancer.To demonstrate the effects of Fucoxanthin (FX), a major active component extracted from Laminaria japonica on metastasis and Gefitinib (Gef) sensitivity in human lung cancer cells both in vitro and in vivo.Invasion and migration of lung cancer cells were det ...
- DOI:
- 10.1016/j.jep.2020.113302
- https://dx.doi.org/10.1016/j.jep.2020.113302