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1. Human mammary epithelial cells in a mature, stratified epithelial layer flatten and stiffen compared to single and confluent cells

Author:
Keith Bonin,   et al.   ; Hyunsu Lee; Martin Guthold;     Show all 3 Authors
Source:
Biochimica et biophysica acta 2021 v.1865 no.6 pp. 129891
ISSN:
0304-4165
Subject:
actin; breasts; confocal microscopy; epithelium; fluorescence; homeostasis; humans; morphogenesis
Abstract:
... The epithelium forms a protective barrier against external biological, chemical and physical insults. So far, AFM-based, micro-mechanical measurements have only been performed on single cells and confluent cells, but not yet on cells in mature layers. Using a combination of atomic force, fluorescence and confocal microscopy, we determined the changes in stiffness, morphology and actin distribution ...
DOI:
10.1016/j.bbagen.2021.129891